Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0003184

Physical Location & Seq

Genomic Coordinates Reverse (-)

16027495 .. 16037635

Gene Structure 10141 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4299 bp

ATGCGATACTTATGCCGTTATTACTTTTCAAATCGTCGTTTTGTTTCTTTCTTTTTCATTGTTATTGGAGTGGTTGTTGGCTGGGTTCCTCTCGCTTTGCTCACAGAAATGGCCAACGAAAGAGACGCAGCGGCAGGAAGCGGTGGCAGCCTAGTTTTTGCAGTTAACGGAGAGAGGTTTGAGCTCCCGACCGTTGATCCTTCAACCACTCTGCTCGCCTTCTTGCGTTCTCAGACGAGATTCAAAAGTGTCAAGCTCAGCTGCGGTGAAGGTGGTTGCGGTGCTTGTGTTGTGCTACTGTCCAAGTACGACCCTGTTGTCGACGAAGTGAAAGATTTTACTGCAAGTTCATGTCTCACTCTGCTTTGCAGTATAAATGGATGTTCAATTACAACATCCGAAGGCCTTGGAAATATCAAAGACGGGTTCCACCCAATTCACCAAAGGTTTGCTGGTTTCCATGCTTCTCAATGCGGCTTTTGTACTCCTGGCATGTGTGTTTCACTCTTTGGAGCTCTTGTCAATGCTGAAAAGGCGAATAGACCTGATCCCCCTCCTGGGTTCTCCAAGCTTTCTGTTTCCGAAGCTGAAAAGTCTATTGCAGGTAACCTCTGTCGATGTACTGGGTATCGACCAATTGCTGATGCCTGCAAGAGTTTCGCATCTGATGTTGACATGGAGGATTTGGGATTCAACTCCTTTTGGAAAAAGGGAGATAGTAAGGAAGTAAAAGTAAAGAGTTTGCCTCTATATAACCGTAATGGTGACATCTGTACATTTCCGGACTTCTTAAAAAAAGAAATTTGTTCTTCCATGTCTTTGGATCCTGCGAGATATGGTTGGTACAATCCTGTCAGAGTTGAGGAGCTTCAAAACTTATTGAAAGATAATGATTTTGATAATGCAAACGAGATGAAATTAGTTGTTGGAAACACAGGGACGGGCTATTACAAGGAGCTAAAGCGCTATGACAGATACATTGATCTCAGATGTGTTCCTGAATTATCAATGGTTAAGAAGGACCCTGTGGGGGTTGAGTTCGGAGCAACTGTAACAATCTCTAAAGTTATTGAGTCTTTGAAGAAGAAAGACAACGGTGGATCTCCATCAAGAGGTGGTGATGTTTCAAAAAAAATTGCCAATCATATGGAGAAAATTGCTTCAGGGTTCATCAGAAATACAGGTAGTATAGGAGGGAATTTGGTGATGGCACAGAGAAAATGTTTTCCTTCAGATATTGCTACAATACTTCTTGCTGTGGATTCAGAAGTAGATATAATGAATGGTGCTAGATCTGAAACGGTTATGTTAGAGGATTTTCTGAAAAGGCCTCCATTGGATCCGAAAAGTGTACTTTTAAGTGTTAAAATCCCAAATATGGAAGCAGTTAGAAAAGTTTCTCCAGAAACCAACACCATGTTGCTGTTTGAAACCTATCGAGCTGCACCAAGACCCCTAGGAAGGGCATTAGCTTATTTAAATGCTGCCTTCCTAGCAGAGGTTTCGTTCGGTAAGATTTCCAATGGAATTATGGTGGATCATTGTCGTCTGGCTTTTGGTGCTTATGGAACTAAACATGCAATTAGAGCAAGAAAGGTCGAGGAATTTTTAACTGGAAAAGTGCTGAGTCCTGGTGTTCTATATGACGCTATTAAATTAGTTAGGGATGTTGTGGTTCCTGAAGAAGGAACTACGAGCCCTGCTTACAGGTCAAGCTTGGCTGCCGGTTTTCTTTTCGAGTTCTTTAGCCCCTTAATTGACAGTGAAAGTTGCAAAGGATTCTTGGGAAATACTTTGTTGGCCGATGCTTCCAAGTTAAAAAGGAACAAAAGTGCTACCGATAAGATGAGAACTGTTCTGTCATCTGGTAAACAGGTGATTGAATTAAGTACAGAGTATGATCCGGTTGGTAAACCAATTACAAAATCTGGAGGTCTCCTACAAGCTTCAGGTGAGGCAGTCTATGTAGACGACATTCCATCTCCGAAAAATTGCTTACATGGAGCGTTCATTTATAGTACAAAGCCTTTGGCTAGAGTGAAGGGAATAAAATTTGAACCTAAATCACATCCAGGAGTTACTGCACTGATATCTTTCAAAGATATTCCCAAGTCTGGGGAGAACATTGGGTCTAAGAATTTTCTTGGTACAGAACCTCTGTTTGCTAATGATCTTACAGAATGGGCTGGTCAGCGTCTTGCTTTCATGGTAGCAGATACGCAGAAACATGCAGATATGGCTACAAACTTTGCGGTGGTTGATTATAACATGGAAGATGTAGATCCACCTATCTTATCAGTCGAAGAGGCAGTTAAGAGAGCAAGTTTTTTTGAGGTCCCTCCTTTCTTGTATCCGAAACAGGTCGGTGACATATCAAATGGAATGGCTGCAGCTGATCACAAGATTATTTCCGCTGAGATCAAACTTCGTTCACAATACTATTTCTACATGGAGACACAAACAGCCCTTGCTGTACCAGATGAAGACAACTGCATGGTGGTTTACACTTCAAGTCAAGTGCCTGAGTATGCACACACTACAATTGCAAAATGTCTTGGTATTCCTGAAAATAATATTCGTGTAATTACAAGAAGGGTCGGAGGGGGCTTTGGTGGAAAGGCCATGCAATCAATGCCTGTCGCAACTGCATGTGCTCTTGCAGCACACAAATTACATAGACCTGTACGGATATATCTCAATCGCAAGACTGATATGATAATGGCTGGAGGAAGGCATCCCATGAAGATAACTTACAGTGTGGGATTCAAGTCTGATGGGAAGATTACAGCTTTAGAACTTGAGATATTGATAAATGCAGGGATTTCCATAGACTTTAGTCCATTATTGCCAAAAAGTATTGTGACTGCACTTAAGAAGTATGACTGGGGTGCTCTGGCTTTCGATATCAAGGTGTGCAAAACAAACACTCCAAGTAGGTCTGCTATGCGGGCCCCTGGGGAGGTGCAGGGGTCATTTATCGCTGAAGCTGTACTTGAACATGTAGCATCCATCCTTTCTATAGAAGTAGATTCTGTGAGAAGCGTTAATCTTCACACATCTCACAGCCTTGATTTGTTTCACAAGCAGAGCGCTGGTGAACCCCTGGAGTATACTTTACCATTGATATGGAACAAGTTGTCCATGTCTTCAAGCTTTAACCCAAGGACTGAGATTGTAAAAGAATTTAATAGGTGTAATAAATGGCAGAAAAGAGGAATTTCTCGAGTACCGATATTGTATGAAGTGTCATTGAGGCCAACTCCGGGAAAAGTAGGCATTTTAAGTGATGGGTCTGTTGTTGTTGAAGTTGGAGGAGTTGAACTGGGTCAGGGACTCTGGACGAAGGTAAAACAGGTGACAGCTTTTGCTCTTGGTTCGATTCAATGTGATGGTAGTGGAGATTTCTTGGATAAAATACGTGTGGTGCAATCAGATACATTGAGCTTAATTCAAGGGGGAATGACTGCTGGGAGCACTACATCTGAGACAAGCTGTGAGGCAGTTAGACTTTGCTGTAATATTTTGGTTGAGAGGTTAGCTACTCTCAAGGGGAGATTGAAGGAGCAAATGGGCTCTATTAACTGGGAGACTCTTATTCAGCAGGCATCCTTGCAAGATGTGAACTTATCAGCAAGTTCTTATTATGTCCCTGATTCTGCTTCCAGGGCATACCTTAACTATGGTGCTGCTGTGAGTGAGGTGGAGGTAAACGTTCTGACTGGACAAACCACAATTCTGCGATCGGATATTATCTACGATTGTGGTCAGAGTCTCAACCCTGCTGTGGATTTAGGACAGATCGAAGGTTCTTTTGTTCAGGGTATTGGGTTTTTCATGCTTGAAGAATACTCGGAAAACTCTGATGGGTTGGTGATTTCAGAAGGTACATGGACATATAAGATTCCTACGGTGGACACCATACCCAAACAGTTCAATGTTGAAGTATTGAACAGTGGACATCATAAAAAACACGTGCTCTCTTCAAAAGCTTCTGGTGAGCCACCATTACTTCTTGCGGTTTCTGTTCACTGCGCTACAAGGGCTGCCATTAAAGAATCAAGAAAACAGCTTCTTCAGTGGGGCGGCCTAGATGGATCGGCATCAATATTCCAGTTAGATGTTCCTGCTACCATGCCTGTTGTGAAGGAGCTGTGTGGACTGGAAGCTGTGGAGAGGTACTTGGAATGGATTGTAGACCAAGGTTCATTCGACCGGAGAATGGTTCGTAATCTCGGAGATTGGGTTGTTTTCTGTCTATATTTATATTTGAGGAAAATTCTATGCAGTGAGAGTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00380 ko00906 ko01100 ko01110 map00380 map00906 map01100 map01110

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1433

Amino Acids

156.56

Weight (kDa)

7.75

Isoelectric Point (pI)

35.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	54 - 120	9.3e-08	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	132 - 218	1.1e-21	[2Fe-2S] binding domain
FAD_binding_5	PF00941	278 - 459	5.5e-39	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	478 - 583	2.6e-19	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	650 - 757	2.8e-27	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	786 - 1017	3.3e-82	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1048 - 1308	5.4e-71	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04580 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT3G43600
fragaria_vesca	FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980
malus_domestica	MD00G1162900.v1.1 MD03G1162200.v1.1 MD11G1178400.v1.1 MD11G1178500.v1.1
prunus_persica	Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1
pyrus_communis	pycom03g11520 pycom11g15240 pycom11g15280 pycom11g15360
rosa_chinensis	RchiOBHm_Chr5g0049511
rosa_laevigata	RLG00000034622
rosa_multiflora	Rmu_sc0001307.1_g000031
rosa_roxburghii	Rroxscaffold_1G00032200
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325300 Rh5BG336500 Rh5CG361200 Rh5DG348600
rosa_wichuraiana	Rw5G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2265
AasI	GACNNNNNNGTC	1 cut(s)	317
Acc36I	ACCTGC	1 cut(s)	593
AccI	GTMKAC	4 cut(s)	321, 1968, 3110, 4194
AccIII	TCCGGA	1 cut(s)	781
AclI	AACGTT	1 cut(s)	3711
AcoI	YGGCCR	2 cut(s)	111, 1800
AcsI	RAATTY	8 cut(s)	801, 1198, 1604, 2051, 2137, 3182, 3216, 4275
AcuI	CTGAAG	6 cut(s)	1146, 1215, 1701, 1932, 3003, 4058
AcvI	CACGTG	1 cut(s)	3973
AfeI	AGCGCT	2 cut(s)	965, 3091
AflII	CTTAAG	1 cut(s)	2870
AflIII	ACRYGT	3 cut(s)	2998, 3418, 3970
AjnI	CCWGG	7 cut(s)	487, 556, 1630, 2071, 2953, 3102, 3662
AloI	GAACNNNNNNTCC	2 cut(s)	3122, 3154
Alw21I	GWGCWC	6 cut(s)	186, 517, 2656, 2893, 3476, 3978
Alw26I	GTCTC	7 cut(s)	117, 359, 1940, 2447, 3479, 3581, 3776
Ama87I	CYCGRG	1 cut(s)	3222
Aor13HI	TCCGGA	1 cut(s)	781
Aor51HI	AGCGCT	2 cut(s)	965, 3091
AoxI	GGCC	8 cut(s)	111, 403, 1328, 1800, 2619, 2949, 3254, 4084
ApaI	GGGCCC	1 cut(s)	2953
ApoI	RAATTY	8 cut(s)	801, 1198, 1604, 2051, 2137, 3182, 3216, 4275
Asp700I	GAANNNNTTC	1 cut(s)	1396
AspA2I	CCTAGG	1 cut(s)	1456
AspLEI	GCGC	3 cut(s)	966, 3092, 4034
AspS9I	GGNCC	4 cut(s)	1021, 2335, 2949, 2950
AsuC2I	CCSGG	1 cut(s)	3264
AvaI	CYCGRG	1 cut(s)	3222
AvaII	GGWCC	2 cut(s)	1021, 2335
AvrII	CCTAGG	1 cut(s)	1456
BaeGI	GKGCMC	1 cut(s)	2953
BalI	TGGCCA	1 cut(s)	113
BamHI	GGATCC	2 cut(s)	823, 1339
BanII	GRGCYC	5 cut(s)	186, 517, 1700, 2953, 3575
BarI	GAAGNNNNNNTAC	2 cut(s)	2735, 2767
BbrPI	CACGTG	1 cut(s)	3973
BbsI	GAAGAC	2 cut(s)	2490, 3138
Bbv12I	GWGCWC	6 cut(s)	186, 517, 2656, 2893, 3476, 3978
BccI	CCATC	9 cut(s)	1114, 1201, 1987, 2768, 3017, 3281, 3383, 3857, 4085
BcgI	CGANNNNNNTGC	5 cut(s)	28, 640, 674, 1485, 1519
BciT130I	CCWGG	7 cut(s)	489, 558, 1632, 2073, 2955, 3104, 3664
BciVI	GTATCC	1 cut(s)	2361
BclI	TGATCA	1 cut(s)	2395
BcnI	CCSGG	1 cut(s)	3264
BcoDI	GTCTC	7 cut(s)	117, 359, 1940, 2447, 3479, 3581, 3776
BfaI	CTAG	6 cut(s)	152, 1290, 1457, 1493, 2034, 4088
BfmI	CTRYAG	2 cut(s)	2388, 3018
BfoI	RGCGCY	2 cut(s)	967, 3093
BfrI	CTTAAG	1 cut(s)	2870
BfuAI	ACCTGC	1 cut(s)	593
BfuI	GTATCC	1 cut(s)	2361
BglII	AGATCT	1 cut(s)	1292
BlnI	CCTAGG	1 cut(s)	1456
BlpI	GCTNAGC	1 cut(s)	257
Bme1390I	CCNGG	8 cut(s)	489, 558, 1632, 2073, 2955, 3104, 3264, 3664
Bme18I	GGWCC	2 cut(s)	1021, 2335
BmeT110I	CYCGRG	1 cut(s)	3222
BmgT120I	GGNCC	4 cut(s)	1021, 2335, 2949, 2950
BmiI	GGNNCC	9 cut(s)	87, 428, 825, 1023, 1341, 1677, 2337, 2951, 2952
BmrFI	CCNGG	8 cut(s)	489, 558, 1632, 2073, 2955, 3104, 3264, 3664
BmrI	ACTGGG	4 cut(s)	633, 2893, 3332, 3592
BmsI	GCATC	7 cut(s)	634, 671, 1795, 2743, 3014, 3614, 4109
BmuI	ACTGGG	4 cut(s)	633, 2893, 3332, 3592
BoxI	GACNNNNGTC	1 cut(s)	2835
BpiI	GAAGAC	2 cut(s)	2490, 3138
BplI	GAGNNNNNCTC	4 cut(s)	730, 762, 3244, 3276
BpmI	CTGGAG	4 cut(s)	1386, 1950, 2745, 3125
Bpu1102I	GCTNAGC	1 cut(s)	257
BpuEI	CTTGAG	2 cut(s)	2819, 3530
BpuMI	CCSGG	1 cut(s)	3264
BsaAI	YACGTR	2 cut(s)	3419, 3973
BsaBI	GATNNNNATC	3 cut(s)	1105, 2280, 4099
BsaI	GGTCTC	1 cut(s)	1940
BsaJI	CCNNGG	9 cut(s)	406, 557, 1456, 2953, 2954, 3102, 3161, 3663, 4198
BsaWI	WCCGGW	3 cut(s)	781, 1903, 4212
Bse118I	RCCGGY	1 cut(s)	1724
Bse1I	ACTGG	8 cut(s)	628, 1618, 2888, 3327, 3587, 3724, 4111, 4164
Bse8I	GATNNNNATC	3 cut(s)	1105, 2280, 4099
BseAI	TCCGGA	1 cut(s)	781
BseBI	CCWGG	7 cut(s)	489, 558, 1632, 2073, 2955, 3104, 3664
BseDI	CCNNGG	9 cut(s)	406, 557, 1456, 2953, 2954, 3102, 3161, 3663, 4198
BseGI	GGATG	8 cut(s)	386, 395, 1672, 2068, 2734, 3005, 3009, 3605
BseJI	GATNNNNATC	3 cut(s)	1105, 2280, 4099
BseMII	CTCAG	8 cut(s)	245, 271, 1000, 1616, 2406, 2514, 3159, 3474
BseNI	ACTGG	8 cut(s)	628, 1618, 2888, 3327, 3587, 3724, 4111, 4164
BseRI	GAGGAG	2 cut(s)	878, 3327
BseSI	GKGCMC	1 cut(s)	2953
BseYI	CCCAGC	2 cut(s)	81, 3467
BsgI	GTGCAG	4 cut(s)	1428, 2067, 2850, 2984
Bsh1285I	CGRYCG	3 cut(s)	192, 3743, 4213
BshFI	GGCC	8 cut(s)	113, 405, 1330, 1802, 2621, 2951, 3256, 4086
BsiEI	CGRYCG	3 cut(s)	192, 3743, 4213
BsiHKAI	GWGCWC	6 cut(s)	186, 517, 2656, 2893, 3476, 3978
BsiHKCI	CYCGRG	1 cut(s)	3222
BsiSI	CCGG	5 cut(s)	782, 1725, 1904, 3263, 4213
BslFI	GGGAC	4 cut(s)	952, 2321, 3345, 3632
BsmAI	GTCTC	7 cut(s)	117, 359, 1940, 2447, 3479, 3581, 3776
BsmBI	CGTCTC	1 cut(s)	117
BsmFI	GGGAC	4 cut(s)	952, 2321, 3345, 3632
BsnI	GGCC	8 cut(s)	113, 405, 1330, 1802, 2621, 2951, 3256, 4086
Bso31I	GGTCTC	1 cut(s)	1940
BsoBI	CYCGRG	1 cut(s)	3222
Bsp120I	GGGCCC	1 cut(s)	2949
Bsp1286I	GDGCHC	9 cut(s)	186, 517, 1700, 2656, 2893, 2953, 3476, 3575, 3978
Bsp13I	TCCGGA	1 cut(s)	781
Bsp1407I	TGTACA	1 cut(s)	773
Bsp1720I	GCTNAGC	1 cut(s)	257
BspANI	GGCC	8 cut(s)	113, 405, 1330, 1802, 2621, 2951, 3256, 4086
BspCNI	CTCAG	8 cut(s)	244, 270, 999, 1617, 2407, 2515, 3160, 3475
BspEI	TCCGGA	1 cut(s)	781
BspLI	GGNNCC	9 cut(s)	87, 428, 825, 1023, 1341, 1677, 2337, 2951, 2952
BspMAI	CTGCAG	1 cut(s)	2392
BspMI	ACCTGC	1 cut(s)	593
BspTI	CTTAAG	1 cut(s)	2870
BspTNI	GGTCTC	1 cut(s)	1940
BsrFI	RCCGGY	1 cut(s)	1724
BsrGI	TGTACA	1 cut(s)	773
BsrI	ACTGG	8 cut(s)	628, 1618, 2888, 3327, 3587, 3724, 4111, 4164
BssAI	RCCGGY	1 cut(s)	1724
BssECI	CCNNGG	9 cut(s)	406, 557, 1456, 2953, 2954, 3102, 3161, 3663, 4198
BssNAI	GTATAC	1 cut(s)	3111
BssT1I	CCWWGG	4 cut(s)	406, 1456, 3161, 4198
Bst1107I	GTATAC	1 cut(s)	3111
Bst2UI	CCWGG	7 cut(s)	489, 558, 1632, 2073, 2955, 3104, 3664
Bst6I	CTCTTC	2 cut(s)	2298, 3985
BstAFI	CTTAAG	1 cut(s)	2870
BstAPI	GCANNNNNTGC	1 cut(s)	2632
BstAUI	TGTACA	1 cut(s)	773
BstBAI	YACGTR	2 cut(s)	3419, 3973
BstC8I	GCNNGC	4 cut(s)	216, 649, 2949, 3603
BstEII	GGTNACC	1 cut(s)	605
BstENI	CCTNNNNNAGG	2 cut(s)	1496, 1683
BstF5I	GGATG	8 cut(s)	386, 395, 1672, 2068, 2734, 3005, 3009, 3605
BstH2I	RGCGCY	2 cut(s)	967, 3093
BstHHI	GCGC	3 cut(s)	966, 3092, 4034
BstMAI	GTCTC	7 cut(s)	117, 359, 1940, 2447, 3479, 3581, 3776
BstMCI	CGRYCG	3 cut(s)	192, 3743, 4213
BstNI	CCWGG	7 cut(s)	489, 558, 1632, 2073, 2955, 3104, 3664
BstNSI	RCATGY	5 cut(s)	496, 1580, 2231, 2652, 3002
BstPAI	GACNNNNGTC	1 cut(s)	2835
BstPI	GGTNACC	1 cut(s)	605
BstSCI	CCNGG	8 cut(s)	487, 556, 1630, 2071, 2953, 3102, 3262, 3662
BstSFI	CTRYAG	2 cut(s)	2388, 3018
BstSLI	GKGCMC	1 cut(s)	2953
BstV2I	GAAGAC	2 cut(s)	2490, 3138
BstX2I	RGATCY	5 cut(s)	823, 1100, 1292, 1339, 2281
BstXI	CCANNNNNNTGG	3 cut(s)	820, 1147, 3126
BstYI	RGATCY	5 cut(s)	823, 1100, 1292, 1339, 2281
BstZ17I	GTATAC	1 cut(s)	3111
BsuI	GTATCC	1 cut(s)	2361
BsuRI	GGCC	8 cut(s)	113, 405, 1330, 1802, 2621, 2951, 3256, 4086
BtsCI	GGATG	8 cut(s)	386, 395, 1672, 2068, 2734, 3005, 3009, 3605
BtsI	GCAGTG	2 cut(s)	4027, 4291
BtsIMutI	CAGTG	7 cut(s)	1768, 2084, 2761, 3958, 4027, 4082, 4291
BveI	ACCTGC	1 cut(s)	593
Cac8I	GCNNGC	4 cut(s)	216, 649, 2949, 3603
CfoI	GCGC	3 cut(s)	966, 3092, 4034
Cfr10I	RCCGGY	1 cut(s)	1724
Cfr13I	GGNCC	4 cut(s)	1021, 2335, 2949, 2950
CseI	GACGC	3 cut(s)	134, 1655, 2183
DraI	TTTAAA	1 cut(s)	1479
DrdI	GACNNNNNNGTC	1 cut(s)	317
DseDI	GACNNNNNNGTC	1 cut(s)	317
EaeI	YGGCCR	2 cut(s)	111, 1800
Eam1104I	CTCTTC	2 cut(s)	2298, 3985
EarI	CTCTTC	2 cut(s)	2298, 3985
Ecl136II	GAGCTC	2 cut(s)	184, 515
Eco130I	CCWWGG	4 cut(s)	406, 1456, 3161, 4198
Eco147I	AGGCCT	2 cut(s)	405, 1330
Eco24I	GRGCYC	5 cut(s)	186, 517, 1700, 2953, 3575
Eco31I	GGTCTC	1 cut(s)	1940
Eco32I	GATATC	2 cut(s)	2091, 2905
Eco47I	GGWCC	2 cut(s)	1021, 2335
Eco47III	AGCGCT	2 cut(s)	965, 3091
Eco53kI	GAGCTC	2 cut(s)	184, 515
Eco57I	CTGAAG	6 cut(s)	1146, 1215, 1701, 1932, 3003, 4058
Eco72I	CACGTG	1 cut(s)	3973
Eco88I	CYCGRG	1 cut(s)	3222
Eco91I	GGTNACC	1 cut(s)	605
EcoICRI	GAGCTC	2 cut(s)	184, 515
EcoNI	CCTNNNNNAGG	2 cut(s)	1496, 1683
EcoO109I	RGGNCCY	3 cut(s)	1021, 2335, 2950
EcoO65I	GGTNACC	1 cut(s)	605
EcoRII	CCWGG	7 cut(s)	487, 556, 1630, 2071, 2953, 3102, 3662
EcoRV	GATATC	2 cut(s)	2091, 2905
EcoT14I	CCWWGG	4 cut(s)	406, 1456, 3161, 4198
EcoT38I	GRGCYC	5 cut(s)	186, 517, 1700, 2953, 3575
ErhI	CCWWGG	4 cut(s)	406, 1456, 3161, 4198
Esp3I	CGTCTC	1 cut(s)	117
FalI	AAGNNNNNCTT	2 cut(s)	2976, 3008
FaqI	GGGAC	4 cut(s)	952, 2321, 3345, 3632
FauI	CCCGC	1 cut(s)	2940
FauNDI	CATATG	1 cut(s)	1146
FbaI	TGATCA	1 cut(s)	2395
FblI	GTMKAC	4 cut(s)	321, 1968, 3110, 4194
FokI	GGATG	8 cut(s)	382, 393, 1679, 2055, 2721, 2992, 2996, 3592
FriOI	GRGCYC	5 cut(s)	186, 517, 1700, 2953, 3575
FspBI	CTAG	6 cut(s)	152, 1290, 1457, 1493, 2034, 4088
GlaI	GCGC	3 cut(s)	965, 3091, 4033
GsaI	CCCAGC	2 cut(s)	85, 3471
GsuI	CTGGAG	4 cut(s)	1386, 1950, 2745, 3125
HaeII	RGCGCY	2 cut(s)	967, 3093
HaeIII	GGCC	8 cut(s)	113, 405, 1330, 1802, 2621, 2951, 3256, 4086
HapII	CCGG	5 cut(s)	782, 1725, 1904, 3263, 4213
HgaI	GACGC	3 cut(s)	134, 1655, 2183
HhaI	GCGC	3 cut(s)	966, 3092, 4034
Hin6I	GCGC	3 cut(s)	964, 3090, 4032
HinP1I	GCGC	3 cut(s)	964, 3090, 4032
HincII	GTYRAC	3 cut(s)	166, 322, 673
HindII	GTYRAC	3 cut(s)	166, 322, 673
HindIII	AAGCTT	5 cut(s)	569, 1714, 1944, 3151, 3987
HpaI	GTTAAC	1 cut(s)	166
HpaII	CCGG	5 cut(s)	782, 1725, 1904, 3263, 4213
Hpy99I	CGWCG	2 cut(s)	39, 326
HpyCH4IV	ACGT	3 cut(s)	3418, 3711, 3972
HpySE526I	ACGT	3 cut(s)	3418, 3711, 3972
HspAI	GCGC	3 cut(s)	964, 3090, 4032
Kpn2I	TCCGGA	1 cut(s)	781
Ksp22I	TGATCA	1 cut(s)	2395
KspAI	GTTAAC	1 cut(s)	166
LmnI	GCTCC	9 cut(s)	189, 512, 865, 955, 1043, 2003, 3471, 3562, 4147
LweI	GCATC	7 cut(s)	634, 671, 1795, 2743, 3014, 3614, 4109
MaeI	CTAG	6 cut(s)	152, 1290, 1457, 1493, 2034, 4088
MaeII	ACGT	3 cut(s)	3418, 3711, 3972
MaeIII	GTNAC	7 cut(s)	605, 764, 1051, 2077, 2366, 2860, 3355
MfeI	CAATTG	2 cut(s)	636, 2541
MflI	RGATCY	5 cut(s)	823, 1100, 1292, 1339, 2281
MhlI	GDGCHC	9 cut(s)	186, 517, 1700, 2656, 2893, 2953, 3476, 3575, 3978
MlsI	TGGCCA	1 cut(s)	113
MluNI	TGGCCA	1 cut(s)	113
MlyI	GAGTC	5 cut(s)	1082, 1636, 3327, 3583, 3778
MmeI	TCCRAC	3 cut(s)	907, 2578, 3289
Mox20I	TGGCCA	1 cut(s)	113
MroI	TCCGGA	1 cut(s)	781
MroXI	GAANNNNTTC	1 cut(s)	1396
MscI	TGGCCA	1 cut(s)	113
MslI	CAYNNNNRTG	2 cut(s)	107, 3124
Msp20I	TGGCCA	1 cut(s)	113
MspA1I	CMGCKG	4 cut(s)	131, 261, 2393, 2414
MspCI	CTTAAG	1 cut(s)	2870
MspI	CCGG	5 cut(s)	782, 1725, 1904, 3263, 4213
MspR9I	CCNGG	8 cut(s)	489, 558, 1632, 2073, 2955, 3104, 3264, 3664
MunI	CAATTG	2 cut(s)	636, 2541
MvaI	CCWGG	7 cut(s)	489, 558, 1632, 2073, 2955, 3104, 3664
NciI	CCSGG	1 cut(s)	3264
NdeI	CATATG	1 cut(s)	1146
NlaIV	GGNNCC	9 cut(s)	87, 428, 825, 1023, 1341, 1677, 2337, 2951, 2952
NmuCI	GTSAC	4 cut(s)	764, 2366, 2860, 3355
NspI	RCATGY	5 cut(s)	496, 1580, 2231, 2652, 3002
PaeR7I	CTCGAG	1 cut(s)	3222
PasI	CCCWGGG	1 cut(s)	2954
PceI	AGGCCT	2 cut(s)	405, 1330
PciI	ACATGT	1 cut(s)	2998
PdmI	GAANNNNTTC	1 cut(s)	1396
PfoI	TCCNGGA	2 cut(s)	2071, 3262
Ple19I	CGATCG	1 cut(s)	3743
PleI	GAGTC	5 cut(s)	1081, 1635, 3327, 3583, 3777
PmaCI	CACGTG	1 cut(s)	3973
PmlI	CACGTG	1 cut(s)	3973
PpsI	GAGTC	5 cut(s)	1081, 1635, 3327, 3583, 3777
Ppu21I	YACGTR	2 cut(s)	3419, 3973
PpuMI	RGGWCCY	2 cut(s)	1021, 2335
PscI	ACATGT	1 cut(s)	2998
PshAI	GACNNNNGTC	1 cut(s)	2835
PsiI	TTATAA	1 cut(s)	2265
Psp124BI	GAGCTC	2 cut(s)	186, 517
Psp1406I	AACGTT	1 cut(s)	3711
Psp5II	RGGWCCY	2 cut(s)	1021, 2335
Psp6I	CCWGG	7 cut(s)	487, 556, 1630, 2071, 2953, 3102, 3662
PspCI	CACGTG	1 cut(s)	3973
PspEI	GGTNACC	1 cut(s)	605
PspFI	CCCAGC	2 cut(s)	81, 3467
PspGI	CCWGG	7 cut(s)	487, 556, 1630, 2071, 2953, 3102, 3662
PspN4I	GGNNCC	9 cut(s)	87, 428, 825, 1023, 1341, 1677, 2337, 2951, 2952
PspOMI	GGGCCC	1 cut(s)	2949
PspPI	GGNCC	4 cut(s)	1021, 2335, 2949, 2950
PspPPI	RGGWCCY	2 cut(s)	1021, 2335
PstI	CTGCAG	1 cut(s)	2392
PsuI	RGATCY	5 cut(s)	823, 1100, 1292, 1339, 2281
PvuI	CGATCG	1 cut(s)	3743
PvuII	CAGCTG	2 cut(s)	261, 2393
RseI	CAYNNNNRTG	2 cut(s)	107, 3124
SacI	GAGCTC	2 cut(s)	186, 517
SalI	GTCGAC	1 cut(s)	320
Sau96I	GGNCC	4 cut(s)	1021, 2335, 2949, 2950
SchI	GAGTC	5 cut(s)	1082, 1636, 3327, 3583, 3778
ScrFI	CCNGG	8 cut(s)	489, 558, 1632, 2073, 2955, 3104, 3264, 3664
SduI	GDGCHC	9 cut(s)	186, 517, 1700, 2656, 2893, 2953, 3476, 3575, 3978
SfaNI	GCATC	7 cut(s)	634, 671, 1795, 2743, 3014, 3614, 4109
SfcI	CTRYAG	2 cut(s)	2388, 3018
Sfr274I	CTCGAG	1 cut(s)	3222
SinI	GGWCC	2 cut(s)	1021, 2335
SlaI	CTCGAG	1 cut(s)	3222
SmiI	ATTTAAAT	1 cut(s)	1479
SmiMI	CAYNNNNRTG	2 cut(s)	107, 3124
SmlI	CTYRAG	4 cut(s)	2798, 2870, 3222, 3545
SmoI	CTYRAG	4 cut(s)	2798, 2870, 3222, 3545
SseBI	AGGCCT	2 cut(s)	405, 1330
SspI	AATATT	3 cut(s)	2575, 3520, 4107
SspMI	CTAG	6 cut(s)	152, 1290, 1457, 1493, 2034, 4088
SstI	GAGCTC	2 cut(s)	186, 517
StuI	AGGCCT	2 cut(s)	405, 1330
StyD4I	CCNGG	8 cut(s)	487, 556, 1630, 2071, 2953, 3102, 3262, 3662
StyI	CCWWGG	4 cut(s)	406, 1456, 3161, 4198
SwaI	ATTTAAAT	1 cut(s)	1479
TaiI	ACGT	3 cut(s)	3421, 3714, 3975
TatI	WGTACW	7 cut(s)	482, 620, 773, 1351, 1889, 2018, 2989
TauI	GCSGC	3 cut(s)	134, 477, 4086
TscAI	CASTG	7 cut(s)	1768, 2091, 2761, 3958, 4034, 4082, 4291
TseFI	GTSAC	4 cut(s)	764, 2366, 2860, 3355
Tsp45I	GTSAC	4 cut(s)	764, 2366, 2860, 3355
TspGWI	ACGGA	2 cut(s)	183, 2701
TspRI	CASTG	7 cut(s)	1768, 2091, 2761, 3958, 4034, 4082, 4291
Vha464I	CTTAAG	1 cut(s)	2870
VpaK11BI	GGWCC	2 cut(s)	1021, 2335
XagI	CCTNNNNNAGG	2 cut(s)	1496, 1683
XapI	RAATTY	8 cut(s)	801, 1198, 1604, 2051, 2137, 3182, 3216, 4275
XceI	RCATGY	5 cut(s)	496, 1580, 2231, 2652, 3002
XcmI	CCANNNNNNNNNTGG	2 cut(s)	449, 1528
XhoI	CTCGAG	1 cut(s)	3222
XmaJI	CCTAGG	1 cut(s)	1456
XmiI	GTMKAC	4 cut(s)	321, 1968, 3110, 4194
XmnI	GAANNNNTTC	1 cut(s)	1396
XspI	CTAG	6 cut(s)	152, 1290, 1457, 1493, 2034, 4088

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.