pycom11g15410

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010040
Physical Location & Seq
Forward (+)
16115341 .. 16116766
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1074 bp
ATGGAGGGAAAGCCGTCTTCTGCAAACATTAATCGTGTTTATGAAGACATTGAACCATCAGTTGAATGGGAAAGAGAGGCAGCTTGTGACACTCTCCTCATTTATCTGCACGGATTTAGAAAGGAGAATCTGAGGATTCAAGTAACGTCGTCTCGCAACATAAGGGTATTTGGTGAACGCGCACTTGGTCATGACAACAAGTGGCAGCGTTTCCGAAGGGAATTCCCCATTCCCTCCAACTGCGACACCAACGACATCAGTGCTAGATTTGAGAACGGCATTCTCTACGTCAAGCTTCCAAAGGCAATTGCTCCCGCAGTAGTTGAATCGAGGGACAAACCACAAAAGCCACCCACCACCACAGAAGCTCCTAGGCCACCCGCCACAGAAGCTCCAAAGCCACCCAAAGAAGCTCCCAAGCCACCAGCCGTGGAAGCTCCAAAGCCACCCAAGGAAGCTCCTAAGCCACCAGCCGTGGAAGCTCCAAAGCCACCCAAAGAAGCTCCCAAGCCACCCACCACAGCAACACCTAAGCGTCAAGAGCCCGCACCCACCACAGAAGCGCCTAAGCCTCAAAAGCCCATAAGCACAAGTGATCTGCCACAGCCTCAACAGAGGAGTAATGCTAATCAAGAACAGAACAAGAAAAGCGATGGTGGATCACATTACGTGCCTGAGGTGCCTCCGCGGGCAGCGGAGAAAGAGAAGGAACCAATTCATCATCCAAGTAAGGTGAAGAAGAGTACTGATAGCCATGCGTTAGCTGATGCTAGCAATGCTGCTGCTAAAAAACTTGAAGATAATCGGGCGAAAAAGGTTGAGGAGGATTCGACAAAGGAGGAGTATAAAAGAGCAATATTCGGGCATGAGAACACCGCCGGGGGTGTTGATAGGTGTTATAGAGCACAAGGTTATAACTGCAAGCAGGTGCTTCAAGGCTTGATCATGGAGCTGAAGCAGCAGCCAAGGAAATTGGTAAACTTGGGTCTGGCATTTTTATTTATTCTGGTACTTGGGTTTTATGTCAAACACACAATCACTTCCGTTCGGGAATTCAAACGCGAAGAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

358

Amino Acids

39.91

Weight (kDa)

9.35

Isoelectric Point (pI)

54.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 36 - 105 4.2e-10 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
AarI CACCTGC 1 cut(s) 916
Acc36I ACCTGC 1 cut(s) 916
AccB1I GGYRCC 1 cut(s) 679
AccII CGCG 3 cut(s) 180, 688, 1062
AciI CCGC 7 cut(s) 315, 381, 546, 686, 688, 695, 876
AclWI GGATC 1 cut(s) 667
AcsI RAATTY 2 cut(s) 221, 1052
AcuI CTGAAG 1 cut(s) 974
AfaI GTAC 2 cut(s) 745, 1011
AgsI TTSAA 7 cut(s) 53, 65, 140, 326, 797, 935, 1057
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
Alw21I GWGCWC 1 cut(s) 907
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 1 cut(s) 667
AoxI GGCC 1 cut(s) 374
ApeKI GCWGC 7 cut(s) 80, 205, 692, 779, 782, 958, 961
ApoI RAATTY 2 cut(s) 221, 1052
AseI ATTAAT 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 714
AspA2I CCTAGG 1 cut(s) 371
AspLEI GCGC 2 cut(s) 182, 565
AsuC2I CCSGG 1 cut(s) 880
AsuHPI GGTGA 2 cut(s) 185, 745
AsuNHI GCTAGC 1 cut(s) 770
AvrII CCTAGG 1 cut(s) 371
AxyI CCTNAGG 1 cut(s) 675
BaeI ACNNNNGTAYC 2 cut(s) 1001, 1034
BanI GGYRCC 1 cut(s) 679
BanII GRGCYC 1 cut(s) 546
BbsI GAAGAC 2 cut(s) 9, 51
Bbv12I GWGCWC 1 cut(s) 907
BbvI GCAGC 7 cut(s) 92, 217, 704, 766, 769, 970, 973
BccI CCATC 2 cut(s) 64, 647
BceAI ACGGC 3 cut(s) 292, 413, 458
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BclI TGATCA 1 cut(s) 942
BcnI CCSGG 1 cut(s) 880
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 3 cut(s) 264, 372, 771
BfoI RGCGCY 1 cut(s) 566
BfuAI ACCTGC 1 cut(s) 916
BisI GCNGC 7 cut(s) 81, 206, 693, 780, 783, 959, 962
BlnI CCTAGG 1 cut(s) 371
BlsI GCNGC 7 cut(s) 82, 207, 694, 781, 784, 960, 963
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 1 cut(s) 880
BmiI GGNNCC 2 cut(s) 681, 711
BmrFI CCNGG 1 cut(s) 880
BmsI GCATC 1 cut(s) 757
BmtI GCTAGC 1 cut(s) 774
BpiI GAAGAC 2 cut(s) 9, 51
Bpu10I CCTNAGC 3 cut(s) 462, 531, 567
BpuMI CCSGG 1 cut(s) 880
BsaAI YACGTR 1 cut(s) 670
BsaJI CCNNGG 7 cut(s) 371, 429, 450, 474, 686, 879, 965
BsaXI ACNNNNNCTCC 4 cut(s) 352, 376, 382, 406
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 1 cut(s) 781
BseDI CCNNGG 7 cut(s) 371, 429, 450, 474, 686, 879, 965
BseGI GGATG 1 cut(s) 721
BseMI GCAATG 1 cut(s) 781
BseMII CTCAG 2 cut(s) 122, 666
BseRI GAGGAG 4 cut(s) 86, 631, 836, 854
BseXI GCAGC 7 cut(s) 92, 217, 704, 766, 769, 970, 973
BsgI GTGCAG 1 cut(s) 92
Bsh1236I CGCG 3 cut(s) 180, 688, 1062
BshFI GGCC 1 cut(s) 376
BshNI GGYRCC 1 cut(s) 679
BsiHKAI GWGCWC 1 cut(s) 907
BsiSI CCGG 1 cut(s) 879
BslFI GGGAC 1 cut(s) 347
BsmAI GTCTC 1 cut(s) 156
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 347
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 1 cut(s) 376
Bsp1286I GDGCHC 2 cut(s) 546, 907
Bsp143I GATC 3 cut(s) 595, 659, 942
BspACI CCGC 7 cut(s) 315, 381, 546, 686, 688, 695, 876
BspANI GGCC 1 cut(s) 376
BspCNI CTCAG 2 cut(s) 123, 667
BspFNI CGCG 3 cut(s) 180, 688, 1062
BspHI TCATGA 1 cut(s) 190
BspLI GGNNCC 2 cut(s) 681, 711
BspMI ACCTGC 1 cut(s) 916
BspOI GCTAGC 1 cut(s) 774
BspPI GGATC 1 cut(s) 667
BspT107I GGYRCC 1 cut(s) 679
BsrDI GCAATG 1 cut(s) 781
BssECI CCNNGG 7 cut(s) 371, 429, 450, 474, 686, 879, 965
BssMI GATC 3 cut(s) 595, 659, 942
BssT1I CCWWGG 3 cut(s) 371, 450, 965
Bst4CI ACNGT 1 cut(s) 1071
Bst6I CTCTTC 1 cut(s) 734
BstBAI YACGTR 1 cut(s) 670
BstC8I GCNNGC 4 cut(s) 546, 690, 772, 923
BstDEI CTNAG 5 cut(s) 131, 462, 531, 567, 675
BstDSI CCRYGG 3 cut(s) 429, 474, 686
BstF5I GGATG 1 cut(s) 721
BstFNI CGCG 3 cut(s) 180, 688, 1062
BstH2I RGCGCY 1 cut(s) 566
BstHHI GCGC 2 cut(s) 182, 565
BstKTI GATC 3 cut(s) 598, 662, 945
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 3 cut(s) 595, 659, 942
BstMWI GCNNNNNNNGC 8 cut(s) 389, 434, 479, 541, 577, 679, 776, 958
BstSCI CCNGG 1 cut(s) 878
BstUI CGCG 3 cut(s) 180, 688, 1062
BstV1I GCAGC 7 cut(s) 92, 217, 704, 766, 769, 970, 973
BstV2I GAAGAC 2 cut(s) 9, 51
Bsu36I CCTNAGG 1 cut(s) 675
BsuRI GGCC 1 cut(s) 376
BtgI CCRYGG 3 cut(s) 429, 474, 686
BtgZI GCGATG 1 cut(s) 666
BtsCI GGATG 1 cut(s) 721
BtsIMutI CAGTG 1 cut(s) 265
BveI ACCTGC 1 cut(s) 916
Cac8I GCNNGC 4 cut(s) 546, 690, 772, 923
CciI TCATGA 1 cut(s) 190
CfoI GCGC 2 cut(s) 182, 565
Cfr42I CCGCGG 1 cut(s) 689
CseI GACGC 1 cut(s) 524
Csp6I GTAC 2 cut(s) 744, 1010
CviAII CATG 4 cut(s) 191, 755, 866, 946
CviQI GTAC 2 cut(s) 744, 1010
DdeI CTNAG 5 cut(s) 131, 462, 531, 567, 675
DpnI GATC 3 cut(s) 597, 661, 944
DpnII GATC 3 cut(s) 595, 659, 942
Eam1104I CTCTTC 1 cut(s) 734
EarI CTCTTC 1 cut(s) 734
Eco130I CCWWGG 3 cut(s) 371, 450, 965
Eco24I GRGCYC 1 cut(s) 546
Eco57I CTGAAG 1 cut(s) 974
Eco81I CCTNAGG 1 cut(s) 675
EcoRI GAATTC 2 cut(s) 221, 1052
EcoT14I CCWWGG 3 cut(s) 371, 450, 965
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 3 cut(s) 371, 450, 965
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 4 cut(s) 194, 758, 869, 949
FaqI GGGAC 1 cut(s) 347
FatI CATG 4 cut(s) 190, 754, 865, 945
FauI CCCGC 4 cut(s) 322, 388, 553, 681
FbaI TGATCA 1 cut(s) 942
Fnu4HI GCNGC 7 cut(s) 81, 206, 693, 780, 783, 959, 962
FokI GGATG 1 cut(s) 708
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 7 cut(s) 81, 206, 693, 780, 783, 959, 962
FspBI CTAG 3 cut(s) 264, 372, 771
GlaI GCGC 2 cut(s) 181, 564
GluI GCNGC 7 cut(s) 81, 206, 693, 780, 783, 959, 962
HaeII RGCGCY 1 cut(s) 566
HaeIII GGCC 1 cut(s) 376
HapII CCGG 1 cut(s) 879
HgaI GACGC 1 cut(s) 524
HhaI GCGC 2 cut(s) 182, 565
Hin1II CATG 4 cut(s) 194, 758, 869, 949
Hin6I GCGC 2 cut(s) 180, 563
HinP1I GCGC 2 cut(s) 180, 563
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 4 cut(s) 127, 136, 326, 827
HpaII CCGG 1 cut(s) 879
HphI GGTGA 2 cut(s) 185, 745
Hpy166II GTNNAC 2 cut(s) 176, 979
Hpy188I TCNGA 2 cut(s) 132, 215
Hpy188III TCNNGA 4 cut(s) 191, 539, 632, 1049
Hpy8I GTNNAC 2 cut(s) 176, 979
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 210, 700
HpyCH4III ACNGT 1 cut(s) 1071
HpyCH4IV ACGT 3 cut(s) 146, 288, 669
HpyCH4V TGCA 3 cut(s) 23, 109, 921
HpyF10VI GCNNNNNNNGC 8 cut(s) 389, 434, 479, 541, 577, 679, 776, 958
HpyF3I CTNAG 5 cut(s) 131, 462, 531, 567, 675
HpySE526I ACGT 3 cut(s) 146, 288, 669
Hsp92II CATG 4 cut(s) 194, 758, 869, 949
HspAI GCGC 2 cut(s) 180, 563
Ksp22I TGATCA 1 cut(s) 942
KspI CCGCGG 1 cut(s) 689
Kzo9I GATC 3 cut(s) 595, 659, 942
LmnI GCTCC 9 cut(s) 316, 373, 397, 418, 442, 463, 487, 508, 949
LpnPI CCDG 7 cut(s) 438, 483, 687, 892, 911, 974, 992
Lsp1109I GCAGC 7 cut(s) 92, 217, 704, 766, 769, 970, 973
LweI GCATC 1 cut(s) 757
MaeI CTAG 3 cut(s) 264, 372, 771
MaeII ACGT 3 cut(s) 146, 288, 669
MaeIII GTNAC 2 cut(s) 86, 142
MalI GATC 3 cut(s) 597, 661, 944
MboI GATC 3 cut(s) 595, 659, 942
MboII GAAGA 5 cut(s) 9, 56, 748, 751, 809
MfeI CAATTG 1 cut(s) 306
MhlI GDGCHC 2 cut(s) 546, 907
MluCI AATT 5 cut(s) 221, 306, 714, 971, 1052
MmeI TCCRAC 1 cut(s) 261
MroXI GAANNNNTTC 1 cut(s) 714
MseI TTAA 1 cut(s) 30
MspA1I CMGCKG 2 cut(s) 688, 695
MspI CCGG 1 cut(s) 879
MspR9I CCNGG 1 cut(s) 880
MunI CAATTG 1 cut(s) 306
Mva1269I GAATGC 1 cut(s) 279
MvnI CGCG 3 cut(s) 180, 688, 1062
MwoI GCNNNNNNNGC 8 cut(s) 389, 434, 479, 541, 577, 679, 776, 958
NciI CCSGG 1 cut(s) 880
NdeII GATC 3 cut(s) 595, 659, 942
NheI GCTAGC 1 cut(s) 770
NlaIII CATG 4 cut(s) 194, 758, 869, 949
NlaIV GGNNCC 2 cut(s) 681, 711
NmuCI GTSAC 1 cut(s) 86
PagI TCATGA 1 cut(s) 190
PaqCI CACCTGC 1 cut(s) 916
PctI GAATGC 1 cut(s) 279
PdmI GAANNNNTTC 1 cut(s) 714
PfeI GAWTC 4 cut(s) 127, 136, 326, 827
PkrI GCNGC 7 cut(s) 82, 207, 694, 781, 784, 960, 963
Ppu21I YACGTR 1 cut(s) 670
PshBI ATTAAT 1 cut(s) 30
PsiI TTATAA 1 cut(s) 915
PspN4I GGNNCC 2 cut(s) 681, 711
RsaI GTAC 2 cut(s) 745, 1011
RsaNI GTAC 2 cut(s) 744, 1010
SacII CCGCGG 1 cut(s) 689
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 7 cut(s) 81, 206, 693, 780, 783, 959, 962
Sau3AI GATC 3 cut(s) 595, 659, 942
ScaI AGTACT 1 cut(s) 745
ScrFI CCNGG 1 cut(s) 880
SduI GDGCHC 2 cut(s) 546, 907
SfaNI GCATC 1 cut(s) 757
Sfr303I CCGCGG 1 cut(s) 689
SgrBI CCGCGG 1 cut(s) 689
Sse9I AATT 5 cut(s) 221, 306, 714, 971, 1052
SsiI CCGC 7 cut(s) 315, 381, 546, 686, 688, 695, 876
SspI AATATT 1 cut(s) 858
SspMI CTAG 3 cut(s) 264, 372, 771
StyD4I CCNGG 1 cut(s) 878
StyI CCWWGG 3 cut(s) 371, 450, 965
TaaI ACNGT 1 cut(s) 1071
TaiI ACGT 3 cut(s) 149, 291, 672
TaqI TCGA 2 cut(s) 329, 830
TasI AATT 5 cut(s) 221, 306, 714, 971, 1052
TatI WGTACW 1 cut(s) 743
TfiI GAWTC 4 cut(s) 127, 136, 326, 827
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 1 cut(s) 86
TseI GCWGC 7 cut(s) 80, 205, 692, 779, 782, 958, 961
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 2 cut(s) 57, 707
TspGWI ACGGA 2 cut(s) 126, 1033
TspRI CASTG 1 cut(s) 265
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 2 cut(s) 221, 1052
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XmaJI CCTAGG 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 714
XspI CTAG 3 cut(s) 264, 372, 771
ZrmI AGTACT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.