Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0003672

Physical Location & Seq

Genomic Coordinates Reverse (-)

20936477 .. 20944843

Gene Structure 8367 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2868 bp

ATGATTGTATATGAGCCGGCTCATCTAGCCCGGCCCATGAACTCAAAATTTTCAAATACTTCCTCGAGTTCTTTCTTCGTAGCACTCCTCTGTCTTCTCGCACGACAAATCACGGTACCCGCCTTCTTATCCCAAAACCCAAAACCCCTCTCTCTCCGTGCAGCAGCAGCACTCCTCTCCATGACTTCCCTATTGCTTTCAACCAAGTTTTCTCTCTACCCAAACGGCACATTTCTCCAATTTAAGTTCCCAAACAGACCTCAAAGAATCACCTTCCCCACGAAAACACCGATAGCGTGCTCAGCCACAGAGCCGCCGAAGCGACGACAAAAACCGCCGAAGAAGAAAAAAAGCGTCGCTGACGGTGAGAAAGGGGTCGACCCAGTTGGCTTCCTTACCAAACATGGCATTTCCCATAAGCAATTTGCTCAGTTCCTCCGTGAAAGACACAAATCCGTGAAGGACCTCACTGATGAAATTTTCAATCGTCACGCCAATCTCCGGGATTTGGCTTCTGGGTTTGAGATATTGGGTATGCATCGACACCCGGAACACAGAGAGGACTATATGGAGTGGGCTCCAGGTGCTCGCTACTGTGCACTGGTTGGTGATTTCAACAGGTGGTCCCCTACTGAGAACTGTGCAAGAGAGGGTCATTTTGGCCACGATGACTATGGATACTGGTTCATCATTCTTGAAGATAAGTTGAGGGATGAAGAAAAACCAGATGAGCTCTATTTTCAACAGTATAACTACATCGATGACTATGATAAAGGTGACAGTGGTGTTACCATTGAGGAACTCTTTAAGAAAGCAAATGATGAGTATTGGGAACCTGGAGAAGACCGGTTTATAAAGAACCGTTTTGAAATGCCAGCAAAGTTATATGAGCAGATTTTTGGGCCTAATGGTCCTCAAACATTAGAGGAACTTGAAGAAATACCAGATGCAGAAACTAGATATAAATCTTGGAAAGAACAGCATAAAGATGATATACCTAGTAACGCACCTTGTTATGATGTGATTGACAATGGAAAAGAGTACGACATTTTCAATGTCGTGCTTGATCCTGTGTCACAAGAAAAATTTCGAGGAAAGAAGCCTCCCTTGGCATATTGGTTTGAGACACGCAAAGGAAGGAAGGCATGGTTGAAAAAGTACAGTCCATGCATTCCTCATGGGAGCAAGTACAGGGTCTATTTTAACACTCCTAGTGGGCCATTGGAACGAGTGCCTGCTTGGGCTACTTATGTGCAACCAGAGGCAGAAGGAGAGCAAGCTTTTGCAATACACTGGGAACCACCGCCAGAGTATGCATACAAATGGAAAAATTCATGCCCAAAAGTGCCAAAGTCTTTGCGCATTTATGAATGCCATGTTGGCATAAGTGGATCAGAGCCAAAAATGACTTCTTTCAATGATTTCACAGAGAAGGTCCTTCCTCATGTAAAGGAGGCCGGGTATAATACGATCCAACTGATGGGAGTTATTGAGCACAAAGATTATTTTACTGTTGGCTATAGAGTCACGAATTTATATGCTGTTAGCAGCCGATATGGAACTCCTGATGATTTCAAGCGGTTGGTTGATGAAGCACATGGACTAGGACTGCTGGTTTTTTTAGACATAGTACATTCCTACTCAGCTGCAGATGAGATGGTTGGATTGTCACTATTTGACGGAACAAATGACTGCTACTTCCACACAGGTAAACGGGGACAACACAAGTACTGGGGTACCAGAATGTTTAAATATGGCGATCTGGATGTCCTGCATTTTCTTCTCTCAAATTTGAACTGGTGGGCCACAGAGTACCAAATTGATGGTTTTAATTTCCATTCACTCTCATCGATGATGTACACACACAATGGTTTTGCTTCTTTTACCGGTGACTTGGAGGAGTACTGTAATCAATATGTAGACAAGGACGCATTGCTATACCTCATTTTGGCAAATGAGATACTTCATGTTCTCCATCCGAATATAATAACAATTGCCGAAGATGCAACCTTCTATCCTGGGCTATGTGAACCCATCTCTCAAGGCGGGTTGGGATTTGATTATTGTGTCAATCTCTCTGTATCAGAGACGTGGTCAAATTTCCTTGAGAATGTTCCGGACCATGATTGGAGCATGAGTAAGATTGTCAGCACATTAATGGGCAACAGAAACTATGCTGATAAAATGCTTGTGTATGCTGAAAATCACAACCAGTCAATATCTGGAGGGCGGTCATTTGCGGAGATATTGTTTGGTGAAATCAAGGATGGTTCTCAAGACACGGATAAATTACTGCTCAGAGGTTGTTCCTTGCACAAGATGATCAGGTTAATTACTTTGACAATCAGTGGCCGTGCTTATCTCAACTTCATGGGCAATGAATTTGGGCATCCAGAGTGTGTTGAGTTCCCCATGTCAAGCAACAATTTCTCATTTGCTCTTGCCAACCGTAGGTGGGATCTTCTTGCCAAGGAAGGATTGCATCGGAACTTGTTTATCTTTGATAAGGACTTGATGAAGTTGGATGAAACTGAAAGAGTACTTGCGAGAGTTTTACCGAGCATTCACCATGTGAATGACAATACCATGGTAATAGCTTACCTCAGAGGCACCCTTCTATTTGTATTCAACTTTCACCCAACAGATTCTTATGAAGGATACGGAATAGGCGTAGAAGAGGCTGGAGAGTATCAACTCGTGCTAAATACGGATGAAATAAAGTATGGAGGTCAGGGACTTATAAAGGATGATCAATATTCTAGGAAAACTATTAGCAAAAGAGTCGATGGCCTCCGAAACTGCTTAGAAGTGCCTCTGCCTAGTAGGACTGCCCAGGTTTACAAACTAAGCCGGATCTTGAGAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0007275 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009791 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

956

Amino Acids

109.75

Weight (kDa)

6.1

Isoelectric Point (pI)

36.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	480 - 549	5.9e-08	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	857 - 949	7.7e-20	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20440 AT3G20440 AT3G20440
fragaria_vesca	FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220
malus_domestica	MD11G1212700.v1.1
prunus_persica	Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1
pyrus_communis	pycom11g18770
rosa_chinensis	RchiOBHm_Chr5g0040901 RchiOBHm_Chr5g0040911
rosa_laevigata	RLG00000034012
rosa_multiflora	Rmu_sc0002848.1_g000016 Rmu_ssc0000416.1_g000003
rosa_roxburghii	Rroxscaffold_1G00039790
rosa_rugosa	Rorug05G0188500 Rorug05G0188600 Rorug05G0188600
rosa_samantha	Rh5AG274100 Rh5BG279100 Rh5CG311200 Rh5DG287300
rosa_wichuraiana	Rw5G025750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	854, 2741
Acc16I	TGCGCA	1 cut(s)	1361
Acc65I	GGTACC	2 cut(s)	115, 1736
AccB1I	GGYRCC	3 cut(s)	115, 1736, 2609
AccB7I	CCANNNNNTGG	1 cut(s)	1480
AccI	GTMKAC	2 cut(s)	378, 1920
AccIII	TCCGGA	1 cut(s)	2116
AciI	CCGC	8 cut(s)	120, 314, 335, 1304, 1579, 2046, 2230, 2240
AclWI	GGATC	5 cut(s)	1061, 1399, 1465, 2466, 2861
AcoI	YGGCCR	2 cut(s)	661, 2350
AcsI	RAATTY	8 cut(s)	47, 477, 1085, 1330, 1531, 1789, 2098, 2381
AdeI	CACNNNGTG	1 cut(s)	2572
AfiI	CCNNNNNNNGG	6 cut(s)	501, 508, 1480, 1948, 2451, 2455
AgeI	ACCGGT	2 cut(s)	846, 1886
AjiI	CACGTC	1 cut(s)	2091
AjnI	CCWGG	4 cut(s)	580, 835, 2017, 2832
AjuI	GAANNNNNNNTTGG	6 cut(s)	1091, 1123, 1362, 1393, 1394, 1425
AluBI	AGCT	4 cut(s)	733, 1280, 1646, 2597
AluI	AGCT	4 cut(s)	733, 1280, 1646, 2597
Alw21I	GWGCWC	5 cut(s)	302, 589, 601, 735, 1497
Alw26I	GTCTC	2 cut(s)	1118, 2081
Alw44I	GTGCAC	1 cut(s)	597
AlwI	GGATC	5 cut(s)	1061, 1399, 1465, 2466, 2861
Ama87I	CYCGRG	1 cut(s)	64
Aor13HI	TCCGGA	1 cut(s)	2116
AoxI	GGCC	8 cut(s)	32, 661, 902, 1217, 1455, 1803, 2350, 2788
ApaLI	GTGCAC	1 cut(s)	597
ApeKI	GCWGC	5 cut(s)	161, 164, 167, 1548, 1646
ApoI	RAATTY	8 cut(s)	47, 477, 1085, 1330, 1531, 1789, 2098, 2381
AseI	ATTAAT	1 cut(s)	2156
AsiGI	ACCGGT	2 cut(s)	846, 1886
Asp700I	GAANNNNTTC	1 cut(s)	1086
Asp718I	GGTACC	2 cut(s)	115, 1736
AspLEI	GCGC	1 cut(s)	1362
AspS9I	GGNCC	9 cut(s)	33, 463, 624, 902, 911, 1217, 1435, 1803, 2119
AsuC2I	CCSGG	4 cut(s)	31, 503, 548, 1459
AsuHPI	GGTGA	8 cut(s)	262, 377, 620, 788, 1901, 2267, 2558, 2627
AvaI	CYCGRG	1 cut(s)	64
AvaII	GGWCC	5 cut(s)	463, 624, 911, 1435, 2119
BaeGI	GKGCMC	1 cut(s)	601
BaeI	ACNNNNGTAYC	2 cut(s)	1720, 1753
BalI	TGGCCA	1 cut(s)	663
BanI	GGYRCC	3 cut(s)	115, 1736, 2609
BanII	GRGCYC	2 cut(s)	580, 735
BauI	CACGAG	1 cut(s)	2696
BbsI	GAAGAC	2 cut(s)	86, 849
Bbv12I	GWGCWC	5 cut(s)	302, 589, 601, 735, 1497
BbvI	GCAGC	5 cut(s)	173, 176, 179, 1560, 1633
BccI	CCATC	7 cut(s)	1474, 1651, 1817, 1983, 2042, 2261, 2780
BceAI	ACGGC	2 cut(s)	241, 2337
BcgI	CGANNNNNNTGC	2 cut(s)	2764, 2798
BciT130I	CCWGG	4 cut(s)	582, 837, 2019, 2834
BciVI	GTATCC	2 cut(s)	671, 2651
BclI	TGATCA	2 cut(s)	2322, 2749
BcnI	CCSGG	4 cut(s)	31, 503, 548, 1459
BcoDI	GTCTC	2 cut(s)	1118, 2081
BfaI	CTAG	7 cut(s)	26, 957, 999, 1212, 1604, 2760, 2820
BfmI	CTRYAG	2 cut(s)	1519, 1647
BfuI	GTATCC	2 cut(s)	671, 2651
BglI	GCCNNNNNGGC	1 cut(s)	1380
BisI	GCNGC	6 cut(s)	162, 165, 168, 314, 1549, 1647
BlpI	GCTNAGC	1 cut(s)	301
BlsI	GCNGC	6 cut(s)	163, 166, 169, 315, 1550, 1648
BmcAI	AGTACT	3 cut(s)	1730, 1904, 2541
Bme1390I	CCNGG	8 cut(s)	31, 503, 548, 582, 837, 1459, 2019, 2834
Bme18I	GGWCC	5 cut(s)	463, 624, 911, 1435, 2119
BmeT110I	CYCGRG	1 cut(s)	64
BmgBI	CACGTC	1 cut(s)	2091
BmgT120I	GGNCC	9 cut(s)	33, 463, 624, 902, 911, 1217, 1435, 1803, 2119
BmiI	GGNNCC	7 cut(s)	117, 579, 626, 834, 1299, 1738, 2611
BmrFI	CCNGG	8 cut(s)	31, 503, 548, 582, 837, 1459, 2019, 2834
BmrI	ACTGGG	3 cut(s)	377, 1303, 1741
BmsI	GCATC	5 cut(s)	547, 937, 1993, 2398, 2491
BmuI	ACTGGG	3 cut(s)	377, 1303, 1741
BpiI	GAAGAC	2 cut(s)	86, 849
BpmI	CTGGAG	4 cut(s)	564, 858, 2244, 2703
Bpu1102I	GCTNAGC	1 cut(s)	301
BpuEI	CTTGAG	3 cut(s)	2025, 2126, 2259
BpuMI	CCSGG	4 cut(s)	31, 503, 548, 1459
Bsa29I	ATCGAT	2 cut(s)	759, 1850
BsaBI	GATNNNNATC	1 cut(s)	964
BsaJI	CCNNGG	5 cut(s)	1107, 2018, 2469, 2586, 2832
BsaWI	WCCGGW	3 cut(s)	846, 1886, 2116
BsaXI	ACNNNNNCTCC	6 cut(s)	1445, 1475, 2122, 2152, 2234, 2264
Bsc4I	CCNNNNNNNGG	6 cut(s)	501, 508, 1480, 1948, 2451, 2455
Bse118I	RCCGGY	3 cut(s)	16, 846, 1886
Bse1I	ACTGG	7 cut(s)	383, 606, 686, 1298, 1736, 1802, 2212
Bse3DI	GCAATG	2 cut(s)	1931, 2383
Bse8I	GATNNNNATC	1 cut(s)	964
BseAI	TCCGGA	1 cut(s)	2116
BseBI	CCWGG	4 cut(s)	582, 837, 2019, 2834
BseCI	ATCGAT	2 cut(s)	759, 1850
BseDI	CCNNGG	5 cut(s)	1107, 2018, 2469, 2586, 2832
BseGI	GGATG	8 cut(s)	718, 1771, 1975, 2272, 2389, 2530, 2716, 2752
BseJI	GATNNNNATC	1 cut(s)	964
BseLI	CCNNNNNNNGG	6 cut(s)	501, 508, 1480, 1948, 2451, 2455
BseMI	GCAATG	2 cut(s)	1931, 2383
BseMII	CTCAG	6 cut(s)	315, 443, 624, 1656, 2311, 2617
BseNI	ACTGG	7 cut(s)	383, 606, 686, 1298, 1736, 1802, 2212
BseRI	GAGGAG	3 cut(s)	77, 164, 1913
BseSI	GKGCMC	1 cut(s)	601
BseXI	GCAGC	5 cut(s)	173, 176, 179, 1560, 1633
BsgI	GTGCAG	1 cut(s)	180
BshFI	GGCC	8 cut(s)	34, 663, 904, 1219, 1457, 1805, 2352, 2790
BshNI	GGYRCC	3 cut(s)	115, 1736, 2609
BshTI	ACCGGT	2 cut(s)	846, 1886
BshVI	ATCGAT	2 cut(s)	759, 1850
BsiHKAI	GWGCWC	5 cut(s)	302, 589, 601, 735, 1497
BsiHKCI	CYCGRG	1 cut(s)	64
BsiSI	CCGG	9 cut(s)	17, 31, 502, 548, 847, 1458, 1887, 2117, 2851
BslFI	GGGAC	3 cut(s)	610, 1731, 2748
BslI	CCNNNNNNNGG	6 cut(s)	501, 508, 1480, 1948, 2451, 2455
BsmAI	GTCTC	2 cut(s)	1118, 2081
BsmBI	CGTCTC	1 cut(s)	2081
BsmFI	GGGAC	3 cut(s)	610, 1731, 2748
BsmI	GAATGC	3 cut(s)	1170, 1376, 2562
BsnI	GGCC	8 cut(s)	34, 663, 904, 1219, 1457, 1805, 2352, 2790
BsoBI	CYCGRG	1 cut(s)	64
Bsp1286I	GDGCHC	6 cut(s)	302, 580, 589, 601, 735, 1497
Bsp13I	TCCGGA	1 cut(s)	2116
Bsp1407I	TGTACA	1 cut(s)	1857
Bsp143I	GATC	8 cut(s)	1066, 1391, 1470, 1759, 2322, 2458, 2749, 2853
Bsp1720I	GCTNAGC	1 cut(s)	301
Bsp19I	CCATGG	1 cut(s)	2586
BspACI	CCGC	8 cut(s)	120, 314, 335, 1304, 1579, 2046, 2230, 2240
BspANI	GGCC	8 cut(s)	34, 663, 904, 1219, 1457, 1805, 2352, 2790
BspCNI	CTCAG	6 cut(s)	314, 442, 625, 1655, 2310, 2616
BspDI	ATCGAT	2 cut(s)	759, 1850
BspEI	TCCGGA	1 cut(s)	2116
BspLI	GGNNCC	7 cut(s)	117, 579, 626, 834, 1299, 1738, 2611
BspMAI	CTGCAG	1 cut(s)	1651
BspPI	GGATC	5 cut(s)	1061, 1399, 1465, 2466, 2861
BspT107I	GGYRCC	3 cut(s)	115, 1736, 2609
BsrDI	GCAATG	2 cut(s)	1931, 2383
BsrFI	RCCGGY	3 cut(s)	16, 846, 1886
BsrGI	TGTACA	1 cut(s)	1857
BsrI	ACTGG	7 cut(s)	383, 606, 686, 1298, 1736, 1802, 2212
BssAI	RCCGGY	3 cut(s)	16, 846, 1886
BssECI	CCNNGG	5 cut(s)	1107, 2018, 2469, 2586, 2832
BssMI	GATC	8 cut(s)	1066, 1391, 1470, 1759, 2322, 2458, 2749, 2853
BssSI	CACGAG	1 cut(s)	2696
BssT1I	CCWWGG	3 cut(s)	1107, 2469, 2586
Bst2BI	CACGAG	1 cut(s)	2696
Bst2UI	CCWGG	4 cut(s)	582, 837, 2019, 2834
Bst6I	CTCTTC	1 cut(s)	2670
BstAUI	TGTACA	1 cut(s)	1857
BstC8I	GCNNGC	6 cut(s)	18, 298, 589, 876, 1236, 1278
BstDEI	CTNAG	8 cut(s)	301, 429, 633, 1642, 2297, 2603, 2803, 2846
BstDSI	CCRYGG	1 cut(s)	2586
BstF5I	GGATG	8 cut(s)	718, 1771, 1975, 2272, 2389, 2530, 2716, 2752
BstHHI	GCGC	1 cut(s)	1362
BstKTI	GATC	8 cut(s)	1069, 1394, 1473, 1762, 2325, 2461, 2752, 2856
BstMAI	GTCTC	2 cut(s)	1118, 2081
BstMBI	GATC	8 cut(s)	1066, 1391, 1470, 1759, 2322, 2458, 2749, 2853
BstMWI	GCNNNNNNNGC	7 cut(s)	26, 167, 302, 319, 584, 1380, 2003
BstNI	CCWGG	4 cut(s)	582, 837, 2019, 2834
BstSCI	CCNGG	8 cut(s)	29, 501, 546, 580, 835, 1457, 2017, 2832
BstSFI	CTRYAG	2 cut(s)	1519, 1647
BstSLI	GKGCMC	1 cut(s)	601
BstV1I	GCAGC	5 cut(s)	173, 176, 179, 1560, 1633
BstV2I	GAAGAC	2 cut(s)	86, 849
BstX2I	RGATCY	2 cut(s)	2458, 2853
BstXI	CCANNNNNNTGG	1 cut(s)	1823
BstYI	RGATCY	2 cut(s)	2458, 2853
Bsu15I	ATCGAT	2 cut(s)	759, 1850
BsuI	GTATCC	2 cut(s)	671, 2651
BsuRI	GGCC	8 cut(s)	34, 663, 904, 1219, 1457, 1805, 2352, 2790
BsuTUI	ATCGAT	2 cut(s)	759, 1850
BtgI	CCRYGG	1 cut(s)	2586
BtrI	CACGTC	1 cut(s)	2091
BtsCI	GGATG	8 cut(s)	718, 1771, 1975, 2272, 2389, 2530, 2716, 2752
BtsIMutI	CAGTG	5 cut(s)	468, 599, 787, 1291, 2353
Cac8I	GCNNGC	6 cut(s)	18, 298, 589, 876, 1236, 1278
CfoI	GCGC	1 cut(s)	1362
Cfr10I	RCCGGY	3 cut(s)	16, 846, 1886
Cfr13I	GGNCC	9 cut(s)	33, 463, 624, 902, 911, 1217, 1435, 1803, 2119
ClaI	ATCGAT	2 cut(s)	759, 1850
CseI	GACGC	2 cut(s)	343, 1937
CspAI	ACCGGT	2 cut(s)	846, 1886
DdeI	CTNAG	8 cut(s)	301, 429, 633, 1642, 2297, 2603, 2803, 2846
DpnI	GATC	8 cut(s)	1068, 1393, 1472, 1761, 2324, 2460, 2751, 2855
DpnII	GATC	8 cut(s)	1066, 1391, 1470, 1759, 2322, 2458, 2749, 2853
DraI	TTTAAA	1 cut(s)	1750
DraIII	CACNNNGTG	1 cut(s)	2572
EaeI	YGGCCR	2 cut(s)	661, 2350
Eam1104I	CTCTTC	1 cut(s)	2670
EarI	CTCTTC	1 cut(s)	2670
Ecl136II	GAGCTC	1 cut(s)	733
Eco130I	CCWWGG	3 cut(s)	1107, 2469, 2586
Eco24I	GRGCYC	2 cut(s)	580, 735
Eco47I	GGWCC	5 cut(s)	463, 624, 911, 1435, 2119
Eco53kI	GAGCTC	1 cut(s)	733
Eco88I	CYCGRG	1 cut(s)	64
EcoICRI	GAGCTC	1 cut(s)	733
EcoO109I	RGGNCCY	2 cut(s)	463, 1435
EcoRII	CCWGG	4 cut(s)	580, 835, 2017, 2832
EcoT14I	CCWWGG	3 cut(s)	1107, 2469, 2586
EcoT22I	ATGCAT	3 cut(s)	540, 1172, 1318
EcoT38I	GRGCYC	2 cut(s)	580, 735
ErhI	CCWWGG	3 cut(s)	1107, 2469, 2586
Esp3I	CGTCTC	1 cut(s)	2081
FalI	AAGNNNNNCTT	2 cut(s)	1091, 1123
FaqI	GGGAC	3 cut(s)	610, 1731, 2748
FauI	CCCGC	2 cut(s)	127, 2039
FbaI	TGATCA	2 cut(s)	2322, 2749
FblI	GTMKAC	2 cut(s)	378, 1920
Fnu4HI	GCNGC	6 cut(s)	162, 165, 168, 314, 1549, 1647
FokI	GGATG	8 cut(s)	725, 1778, 1962, 2279, 2376, 2537, 2723, 2759
FriOI	GRGCYC	2 cut(s)	580, 735
Fsp4HI	GCNGC	6 cut(s)	162, 165, 168, 314, 1549, 1647
FspBI	CTAG	7 cut(s)	26, 957, 999, 1212, 1604, 2760, 2820
FspI	TGCGCA	1 cut(s)	1361
GlaI	GCGC	1 cut(s)	1361
GluI	GCNGC	6 cut(s)	162, 165, 168, 314, 1549, 1647
GsuI	CTGGAG	4 cut(s)	564, 858, 2244, 2703
HaeIII	GGCC	8 cut(s)	34, 663, 904, 1219, 1457, 1805, 2352, 2790
HapII	CCGG	9 cut(s)	17, 31, 502, 548, 847, 1458, 1887, 2117, 2851
HgaI	GACGC	2 cut(s)	343, 1937
HhaI	GCGC	1 cut(s)	1362
Hin6I	GCGC	1 cut(s)	1360
HinP1I	GCGC	1 cut(s)	1360
HincII	GTYRAC	1 cut(s)	379
HindII	GTYRAC	1 cut(s)	379
HindIII	AAGCTT	1 cut(s)	1278
HinfI	GANTC	4 cut(s)	267, 1524, 2645, 2781
HpaII	CCGG	9 cut(s)	17, 31, 502, 548, 847, 1458, 1887, 2117, 2851
HphI	GGTGA	8 cut(s)	262, 377, 620, 788, 1901, 2267, 2558, 2627
Hpy166II	GTNNAC	7 cut(s)	379, 599, 1712, 1860, 1921, 2030, 2839
Hpy188I	TCNGA	7 cut(s)	1396, 1980, 2086, 2300, 2487, 2606, 2795
Hpy188III	TCNNGA	9 cut(s)	695, 1528, 1565, 1763, 2117, 2223, 2276, 2393, 2857
Hpy8I	GTNNAC	7 cut(s)	379, 599, 1712, 1860, 1921, 2030, 2839
Hpy99I	CGWCG	2 cut(s)	327, 359
HpyCH4IV	ACGT	1 cut(s)	2090
HpyF10VI	GCNNNNNNNGC	7 cut(s)	26, 167, 302, 319, 584, 1380, 2003
HpyF3I	CTNAG	8 cut(s)	301, 429, 633, 1642, 2297, 2603, 2803, 2846
HpySE526I	ACGT	1 cut(s)	2090
HspAI	GCGC	1 cut(s)	1360
Kpn2I	TCCGGA	1 cut(s)	2116
KpnI	GGTACC	2 cut(s)	119, 1740
KroI	GCCGGC	1 cut(s)	16
KroNI	GCCGGC	1 cut(s)	18
Ksp22I	TGATCA	2 cut(s)	2322, 2749
Kzo9I	GATC	8 cut(s)	1066, 1391, 1470, 1759, 2322, 2458, 2749, 2853
LmnI	GCTCC	3 cut(s)	583, 1182, 2130
Lsp1109I	GCAGC	5 cut(s)	173, 176, 179, 1560, 1633
LweI	GCATC	5 cut(s)	547, 937, 1993, 2398, 2491
MaeI	CTAG	7 cut(s)	26, 957, 999, 1212, 1604, 2760, 2820
MaeII	ACGT	1 cut(s)	2090
MaeIII	GTNAC	8 cut(s)	488, 776, 787, 1001, 1074, 1525, 1668, 1889
MalI	GATC	8 cut(s)	1068, 1393, 1472, 1761, 2324, 2460, 2751, 2855
MboI	GATC	8 cut(s)	1066, 1391, 1470, 1759, 2322, 2458, 2749, 2853
MfeI	CAATTG	1 cut(s)	1992
MflI	RGATCY	2 cut(s)	2458, 2853
MhlI	GDGCHC	6 cut(s)	302, 580, 589, 601, 735, 1497
MlsI	TGGCCA	1 cut(s)	663
MluNI	TGGCCA	1 cut(s)	663
MlyI	GAGTC	2 cut(s)	1533, 2790
MmeI	TCCRAC	3 cut(s)	1498, 1642, 2502
Mox20I	TGGCCA	1 cut(s)	663
Mph1103I	ATGCAT	3 cut(s)	540, 1172, 1318
MroI	TCCGGA	1 cut(s)	2116
MroNI	GCCGGC	1 cut(s)	16
MroXI	GAANNNNTTC	1 cut(s)	1086
MscI	TGGCCA	1 cut(s)	663
MseI	TTAA	7 cut(s)	243, 807, 1203, 1749, 1830, 2156, 2330
MslI	CAYNNNNRTG	4 cut(s)	987, 1321, 2156, 2574
Msp20I	TGGCCA	1 cut(s)	663
MspA1I	CMGCKG	1 cut(s)	1646
MspI	CCGG	9 cut(s)	17, 31, 502, 548, 847, 1458, 1887, 2117, 2851
MspR9I	CCNGG	8 cut(s)	31, 503, 548, 582, 837, 1459, 2019, 2834
MunI	CAATTG	1 cut(s)	1992
Mva1269I	GAATGC	3 cut(s)	1170, 1376, 2562
MvaI	CCWGG	4 cut(s)	582, 837, 2019, 2834
MwoI	GCNNNNNNNGC	7 cut(s)	26, 167, 302, 319, 584, 1380, 2003
NaeI	GCCGGC	1 cut(s)	18
NciI	CCSGG	4 cut(s)	31, 503, 548, 1459
NcoI	CCATGG	1 cut(s)	2586
NdeII	GATC	8 cut(s)	1066, 1391, 1470, 1759, 2322, 2458, 2749, 2853
NgoMIV	GCCGGC	1 cut(s)	16
NlaIV	GGNNCC	7 cut(s)	117, 579, 626, 834, 1299, 1738, 2611
NmuCI	GTSAC	6 cut(s)	488, 776, 1074, 1525, 1668, 1889
NsbI	TGCGCA	1 cut(s)	1361
NsiI	ATGCAT	3 cut(s)	540, 1172, 1318
PaeR7I	CTCGAG	1 cut(s)	64
PcsI	WCGNNNNNNNCGW	2 cut(s)	287, 2667
PctI	GAATGC	3 cut(s)	1170, 1376, 2562
PdiI	GCCGGC	1 cut(s)	18
PdmI	GAANNNNTTC	1 cut(s)	1086
PfeI	GAWTC	2 cut(s)	267, 2645
PflFI	GACNNNGTC	1 cut(s)	2092
PflMI	CCANNNNNTGG	1 cut(s)	1480
PfoI	TCCNGGA	1 cut(s)	501
PinAI	ACCGGT	2 cut(s)	846, 1886
PkrI	GCNGC	6 cut(s)	163, 166, 169, 315, 1550, 1648
PleI	GAGTC	2 cut(s)	1532, 2789
PpsI	GAGTC	2 cut(s)	1532, 2789
PpuMI	RGGWCCY	2 cut(s)	463, 1435
PshBI	ATTAAT	1 cut(s)	2156
PsiI	TTATAA	2 cut(s)	854, 2741
Psp124BI	GAGCTC	1 cut(s)	735
Psp5II	RGGWCCY	2 cut(s)	463, 1435
Psp6I	CCWGG	4 cut(s)	580, 835, 2017, 2832
PspGI	CCWGG	4 cut(s)	580, 835, 2017, 2832
PspN4I	GGNNCC	7 cut(s)	117, 579, 626, 834, 1299, 1738, 2611
PspPI	GGNCC	9 cut(s)	33, 463, 624, 902, 911, 1217, 1435, 1803, 2119
PspPPI	RGGWCCY	2 cut(s)	463, 1435
PspXI	VCTCGAGB	1 cut(s)	64
PstI	CTGCAG	1 cut(s)	1651
PsuI	RGATCY	2 cut(s)	2458, 2853
PsyI	GACNNNGTC	1 cut(s)	2092
PvuII	CAGCTG	1 cut(s)	1646
RseI	CAYNNNNRTG	4 cut(s)	987, 1321, 2156, 2574
SacI	GAGCTC	1 cut(s)	735
SalI	GTCGAC	1 cut(s)	377
SaqAI	TTAA	7 cut(s)	243, 807, 1203, 1749, 1830, 2156, 2330
SatI	GCNGC	6 cut(s)	162, 165, 168, 314, 1549, 1647
Sau3AI	GATC	8 cut(s)	1066, 1391, 1470, 1759, 2322, 2458, 2749, 2853
Sau96I	GGNCC	9 cut(s)	33, 463, 624, 902, 911, 1217, 1435, 1803, 2119
ScaI	AGTACT	3 cut(s)	1730, 1904, 2541
SchI	GAGTC	2 cut(s)	1533, 2790
ScrFI	CCNGG	8 cut(s)	31, 503, 548, 582, 837, 1459, 2019, 2834
SduI	GDGCHC	6 cut(s)	302, 580, 589, 601, 735, 1497
SfaNI	GCATC	5 cut(s)	547, 937, 1993, 2398, 2491
SfcI	CTRYAG	2 cut(s)	1519, 1647
Sfr274I	CTCGAG	1 cut(s)	64
SinI	GGWCC	5 cut(s)	463, 624, 911, 1435, 2119
SlaI	CTCGAG	1 cut(s)	64
SmiMI	CAYNNNNRTG	4 cut(s)	987, 1321, 2156, 2574
SmlI	CTYRAG	5 cut(s)	64, 2040, 2105, 2274, 2857
SmoI	CTYRAG	5 cut(s)	64, 2040, 2105, 2274, 2857
SsiI	CCGC	8 cut(s)	120, 314, 335, 1304, 1579, 2046, 2230, 2240
SspI	AATATT	1 cut(s)	2756
SspMI	CTAG	7 cut(s)	26, 957, 999, 1212, 1604, 2760, 2820
SstI	GAGCTC	1 cut(s)	735
StyD4I	CCNGG	8 cut(s)	29, 501, 546, 580, 835, 1457, 2017, 2832
StyI	CCWWGG	3 cut(s)	1107, 2469, 2586
TaiI	ACGT	1 cut(s)	2093
TaqI	TCGA	7 cut(s)	65, 378, 541, 759, 1090, 1850, 2784
TatI	WGTACW	7 cut(s)	1158, 1188, 1630, 1728, 1857, 1902, 2539
TauI	GCSGC	1 cut(s)	316
TfiI	GAWTC	2 cut(s)	267, 2645
Tru1I	TTAA	7 cut(s)	243, 807, 1203, 1749, 1830, 2156, 2330
Tru9I	TTAA	7 cut(s)	243, 807, 1203, 1749, 1830, 2156, 2330
TscAI	CASTG	5 cut(s)	475, 606, 787, 1298, 2353
TseFI	GTSAC	6 cut(s)	488, 776, 1074, 1525, 1668, 1889
TseI	GCWGC	5 cut(s)	161, 164, 167, 1548, 1646
Tsp45I	GTSAC	6 cut(s)	488, 776, 1074, 1525, 1668, 1889
TspGWI	ACGGA	7 cut(s)	146, 428, 445, 1695, 2297, 2676, 2723
TspRI	CASTG	5 cut(s)	475, 606, 787, 1298, 2353
Tth111I	GACNNNGTC	1 cut(s)	2092
Van91I	CCANNNNNTGG	1 cut(s)	1480
VneI	GTGCAC	1 cut(s)	597
VpaK11BI	GGWCC	5 cut(s)	463, 624, 911, 1435, 2119
VspI	ATTAAT	1 cut(s)	2156
XapI	RAATTY	8 cut(s)	47, 477, 1085, 1330, 1531, 1789, 2098, 2381
XcmI	CCANNNNNNNNNTGG	2 cut(s)	671, 2219
XhoI	CTCGAG	1 cut(s)	64
XmiI	GTMKAC	2 cut(s)	378, 1920
XmnI	GAANNNNTTC	1 cut(s)	1086
XspI	CTAG	7 cut(s)	26, 957, 999, 1212, 1604, 2760, 2820
ZrmI	AGTACT	3 cut(s)	1730, 1904, 2541
Zsp2I	ATGCAT	3 cut(s)	540, 1172, 1318

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.