pycom11g26080

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006473
Physical Location & Seq
Forward (+)
28726353 .. 28731888
5536 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2658 bp
ATGGGGTCGAAGCCATGGCTGTTCCCGGCTCCGACCTATCGTACTCTCGAGACCTTTTGGGATTGCGACGACGACGCCCCTGGTCCACGCTGCGCTCACACCCTCACCGCCGTCGCCGCCACCAAAACCCACGGCCCCCGTCTCATCCTTTTCGGTGGTGCCACCGCCATTGAAGGCGGTGGCCCCTCCTCCGCCCCTGGCATCAGGTTAGCTGGGGTGACCAATTCGGTGCATTCCTATGATATTCTCACCAGGAAATGGACTAGAATGAGGCCTGCAGGTGAGCCACCTTCTCCGAGGGCAGCTCACGCGGCAGCTGCGGTGGGCACTATGGTTGTATTTCAGGGCGGCATAGGTCCTGCTGGGCATTCCACAGATGATCTCTACGTGCTGGACTTAACCAACGATAAATTTAAATGGCACAGAGTGGTTGTACAAGGACAAGGACCCGGGCCTCGCTACGGCCATGTAATGGACTTAGTTGCTCAAAGATATCTTGTTACCGTCAGCGGAAATGATGGAAAAAGAGTTCTTTCTGATGCTTGGGCTCTGGATACTGCTCAAAAACCTTATGCATGGGCTAGGCTGAACCCAGAAGGTGATAGACCATCTGCTAGAATGTATGCAACAGCCAGTGCTCGCTCAGATGGTATGTTTTTGCTTTGTGGTGGAAGAGATATTTCTGGAACGCCTCTAGCAGACGCTTATGGACTGCTTATGCATAGGAATGGTCAGTGGGAGTGGACCCTTGCACCTGGTGTATCCCCTTCACCAAGATATGAACATGCGGCGGTTTTTGTTGGCGCAAGATTACATGTTACAGGAGGTGCTCTTAGGGGAGGTCGTGGTATAGAAGGTGAAGCAGCTATTGCAGTATTGGACACTGCTGCTGGAGTTTGGTTAGACAGAAATGGGCTTGTGACTTCCTCACGCACAGGAAAGGGACAGAATGACCATGATCCTTCTTTGGAGCTCATGCGTCGTTTTCGCCACGCAGCTGCATCTCTTGGTTTTCGTATATATATTTATGGTGGTCTTAGGGGAGACATCCTGCTAGATGACTTCCTGGTTGCAGAAAATTCATCAGTTCAGTCTGAAGGTAATTTGCCTGTTTTAACATCTGAGAGATCCCCAACTGTATCCAGTCCCAGAATGTTTCATGCTAACACAAGTACTTTTGGAACATCTTCTGATGGTGAACCTGAGAGTACCTCATCAACTGGCTTGAGCATGGACAAAAATTCAATGGAGAAACTCCGGGAGGCTTCTGCTGCCGAAGCTGAGGCAGCCAGTGCTGTTTGGCAAGCTGCGCAATCAGCATCTGCTACTCCTTCTGAAGAAACATCTGTTTCAGATGATAACTCACAAGTTGCAGAAACATTGTCTGATGGTAGTGACACCGAGGCAGATGTTCGTCTTCATCACAGAGCTGTTGTCGTTGCCAAAGAGGCTGTAGGCAACTTGGGTGGGATGGTGAGACAGTTGTCTTTGGATCAATTTGAAAATGAGAGCAGACGAACGATTCCAATGAACAATGACTTATCACATCCTAACAAAAAGTACACCAGGCAGAAGTCTCCACAAGGGCTACATAAGAAGGTTATATCTACATTGTTGAGGCCTCGAAACTGGAAACCCCCTGTCAATAGGAAGTTTTTCCTGGATTCTTATGAAGTGGGAGAACTTTGTTATGCTGCTGAGCAGATATTTATGCAGGAGCAAACAGTTCTTCAGCTGAAAGCCCCTGTTAAAGTATTTGTCGATCTTCATGGACAGTTTGGTGATTTAATGCGCTTATTTGATGAATATGGATTTCCATCTGCTGCGGGAGACATAACGTATATCGACTACTTGTTTTTGGGGGATTATGTCGATCGAGGACAGCACAGCTTGGAGACCATAACTTTGCTCCTTGCACTGAAGATCGAGTATCCTGACAATGTTCACTTAATACGTGGAAACCATGAAGCTGCTGATATCAATGCACTCTTTGGTTTTCGTATTGAATGCGTTGAGAGAATGGGCGAGAATGATGGAATCTGGGCATGGACTCGGTTCAATCAACTTTTCAACTATCTCCCACTTGCTGCTCTGATTGAAAAGAAAATTATCTGTATGCATGGAGGCATTGGGAGATCTATCAATTCTGTAGAACAGATAGAGAAGCTTGAAAGACCCATTACAATGGATGCTGGCTCTATAATTTTAATGGATCTTTTGTGGTCTGATCCCACAGAAAACGACAGTGTAGAGGGCTTGAGACCAAATGCCAGAGGACCTGGTCTTGTCACCTTTGGGCCTGATCGTGTCACAGAATTTTGTAAGAGAAACAAATTACAACTCATTGTAAGAGCTCATGAATGTGTCATGGATGGATTTGAAAGATTTGCCCAGGGACAGTTGATCACTCTTTTTTCTGCAACCAACTATTGCGGGACGGCAAACAATGCTGGTGCTATACTGGTCGTTGGCAGAGGTTTGGTTGTGGTTCCAAAATTAATCCATCCTTTGCCACCTCCTCTTCAGTCACCGGAGACATCTCCTGAACGGGTCATAGACGACGCATGGATGCAGGAGCTCAACATTCAAAGACCACCAACGCCTACTCGTGGTCGACCGCAGCCTGATCTTGATCGAAACTCACTTGCATATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4 Metallophos STPPase_N

Protein Analysis

886

Amino Acids

96.74

Weight (kDa)

5.97

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 23 - 188 2.3e-24 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 35 - 276 1.3e-38 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 72 - 347 8.2e-15 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 74 - 352 7.8e-19 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 175 - 356 3.2e-12 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 203 - 277 8e-06 tRNA wybutosine-synthesizing protein 4, beta-propeller
Metallophos PF00149 583 - 789 2.7e-37 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 269
Acc16I TGCGCA 1 cut(s) 1311
Acc36I ACCTGC 1 cut(s) 269
AccB1I GGYRCC 1 cut(s) 158
AccB7I CCANNNNNTGG 2 cut(s) 258, 472
AccI GTMKAC 1 cut(s) 2614
AccII CGCG 1 cut(s) 311
AclWI GGATC 5 cut(s) 953, 1122, 1500, 2220, 2222
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 4 cut(s) 410, 1078, 1240, 2315
AcuI CTGAAG 5 cut(s) 1116, 1356, 1715, 1940, 2507
AcyI GRCGYC 1 cut(s) 75
AdeI CACNNNGTG 2 cut(s) 427, 758
AfaI GTAC 5 cut(s) 43, 435, 1174, 1210, 1562
AfiI CCNNNNNNNGG 4 cut(s) 258, 461, 472, 2609
AflIII ACRYGT 1 cut(s) 814
AjnI CCWGG 9 cut(s) 79, 196, 251, 754, 1065, 1565, 1659, 2278, 2391
AloI GAACNNNNNNTCC 2 cut(s) 513, 545
Alw21I GWGCWC 5 cut(s) 640, 832, 975, 2356, 2580
Alw26I GTCTC 9 cut(s) 44, 146, 1038, 1471, 1581, 1824, 1889, 2254, 2528
AlwI GGATC 5 cut(s) 953, 1122, 1500, 2220, 2222
AlwNI CAGNNNCTG 1 cut(s) 1322
Ama87I CYCGRG 2 cut(s) 47, 449
AoxI GGCC 7 cut(s) 133, 181, 272, 452, 463, 1619, 2297
ApoI RAATTY 4 cut(s) 410, 1078, 1240, 2315
ArsI GACNNNNNNTTYG 2 cut(s) 1888, 1920
AseI ATTAAT 1 cut(s) 2498
Asp700I GAANNNNTTC 2 cut(s) 1186, 1655
AspLEI GCGC 4 cut(s) 95, 806, 1312, 1794
AspS9I GGNCC 9 cut(s) 83, 134, 182, 356, 446, 452, 744, 2276, 2297
AsuC2I CCSGG 4 cut(s) 26, 450, 451, 1259
AvaI CYCGRG 2 cut(s) 47, 449
AvaII GGWCC 5 cut(s) 83, 356, 446, 744, 2276
BaeGI GKGCMC 1 cut(s) 329
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 4 cut(s) 550, 975, 2356, 2580
BauI CACGAG 1 cut(s) 2607
BbsI GAAGAC 1 cut(s) 1409
Bbv12I GWGCWC 5 cut(s) 640, 832, 975, 2356, 2580
BbvCI CCTCAGC 1 cut(s) 1281
BceAI ACGGC 4 cut(s) 95, 148, 478, 2454
BciT130I CCWGG 9 cut(s) 81, 198, 253, 756, 1067, 1567, 1661, 2280, 2393
BciVI GTATCC 4 cut(s) 547, 772, 1150, 1941
BclI TGATCA 1 cut(s) 2403
BcnI CCSGG 4 cut(s) 26, 450, 451, 1259
BcoDI GTCTC 9 cut(s) 44, 146, 1038, 1471, 1581, 1824, 1889, 2254, 2528
BfaI CTAG 5 cut(s) 264, 582, 615, 695, 1055
BfmI CTRYAG 3 cut(s) 276, 1452, 2148
BfuAI ACCTGC 1 cut(s) 269
BfuI GTATCC 4 cut(s) 547, 772, 1150, 1941
BglI GCCNNNNNGGC 1 cut(s) 1448
BglII AGATCT 1 cut(s) 2135
BlpI GCTNAGC 1 cut(s) 1698
BmcAI AGTACT 1 cut(s) 1174
Bme18I GGWCC 5 cut(s) 83, 356, 446, 744, 2276
BmeT110I CYCGRG 2 cut(s) 47, 449
BmgT120I GGNCC 9 cut(s) 83, 134, 182, 356, 446, 452, 744, 2276, 2297
BmiI GGNNCC 7 cut(s) 30, 136, 160, 184, 448, 746, 2490
BmsI GCATC 6 cut(s) 210, 529, 1010, 1328, 2179, 2559
BoxI GACNNNNGTC 1 cut(s) 1483
BpiI GAAGAC 1 cut(s) 1409
BplI GAGNNNNNCTC 4 cut(s) 289, 321, 1196, 1228
BpmI CTGGAG 1 cut(s) 912
Bpu10I CCTNAGC 1 cut(s) 1281
Bpu1102I GCTNAGC 1 cut(s) 1698
BpuEI CTTGAG 2 cut(s) 1246, 2278
BpuMI CCSGG 4 cut(s) 26, 450, 451, 1259
BsaAI YACGTR 2 cut(s) 388, 1955
BsaBI GATNNNNATC 2 cut(s) 1816, 2631
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 3 cut(s) 44, 1889, 2254
BsaJI CCNNGG 9 cut(s) 14, 79, 130, 196, 296, 449, 1401, 2391, 2392
BsaWI WCCGGW 1 cut(s) 2530
Bsc4I CCNNNNNNNGG 4 cut(s) 258, 461, 472, 2609
Bse1I ACTGG 6 cut(s) 633, 1143, 1225, 1290, 1634, 2466
Bse8I GATNNNNATC 2 cut(s) 1816, 2631
BseBI CCWGG 9 cut(s) 81, 198, 253, 756, 1067, 1567, 1661, 2280, 2393
BseDI CCNNGG 9 cut(s) 14, 79, 130, 196, 296, 449, 1401, 2391, 2392
BseGI GGATG 8 cut(s) 144, 1047, 1476, 1546, 2194, 2377, 2503, 2574
BseJI GATNNNNATC 2 cut(s) 1816, 2631
BseLI CCNNNNNNNGG 4 cut(s) 258, 461, 472, 2609
BseMII CTCAG 5 cut(s) 657, 1113, 1194, 1272, 1689
BseNI ACTGG 6 cut(s) 633, 1143, 1225, 1290, 1634, 2466
BseRI GAGGAG 2 cut(s) 178, 2508
BseSI GKGCMC 1 cut(s) 329
BseYI CCCAGC 2 cut(s) 212, 362
Bsh1236I CGCG 1 cut(s) 311
Bsh1285I CGRYCG 2 cut(s) 1876, 2618
BshFI GGCC 7 cut(s) 135, 183, 274, 454, 465, 1621, 2299
BshNI GGYRCC 1 cut(s) 158
BsiEI CGRYCG 2 cut(s) 1876, 2618
BsiHKAI GWGCWC 5 cut(s) 640, 832, 975, 2356, 2580
BsiHKCI CYCGRG 2 cut(s) 47, 449
BsiSI CCGG 4 cut(s) 26, 450, 1258, 2531
BslFI GGGAC 4 cut(s) 957, 1131, 2409, 2449
BslI CCNNNNNNNGG 4 cut(s) 258, 461, 472, 2609
BsmAI GTCTC 9 cut(s) 44, 146, 1038, 1471, 1581, 1824, 1889, 2254, 2528
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 4 cut(s) 957, 1131, 2409, 2449
BsmI GAATGC 3 cut(s) 232, 367, 2012
BsnI GGCC 7 cut(s) 135, 183, 274, 454, 465, 1621, 2299
Bso31I GGTCTC 3 cut(s) 44, 1889, 2254
BsoBI CYCGRG 2 cut(s) 47, 449
Bsp1286I GDGCHC 7 cut(s) 329, 550, 640, 832, 975, 2356, 2580
Bsp1407I TGTACA 1 cut(s) 433
Bsp1720I GCTNAGC 1 cut(s) 1698
Bsp19I CCATGG 1 cut(s) 14
BspANI GGCC 7 cut(s) 135, 183, 274, 454, 465, 1621, 2299
BspCNI CTCAG 5 cut(s) 656, 1114, 1195, 1273, 1690
BspFNI CGCG 1 cut(s) 311
BspHI TCATGA 1 cut(s) 2356
BspLI GGNNCC 7 cut(s) 30, 136, 160, 184, 448, 746, 2490
BspMAI CTGCAG 1 cut(s) 280
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 5 cut(s) 953, 1122, 1500, 2220, 2222
BspT107I GGYRCC 1 cut(s) 158
BspTNI GGTCTC 3 cut(s) 44, 1889, 2254
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 6 cut(s) 633, 1143, 1225, 1290, 1634, 2466
BssECI CCNNGG 9 cut(s) 14, 79, 130, 196, 296, 449, 1401, 2391, 2392
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 2607
BssT1I CCWWGG 1 cut(s) 14
Bst2BI CACGAG 1 cut(s) 2607
Bst2UI CCWGG 9 cut(s) 81, 198, 253, 756, 1067, 1567, 1661, 2280, 2393
Bst4CI ACNGT 7 cut(s) 505, 1138, 1482, 1726, 1776, 2246, 2400
Bst6I CTCTTC 2 cut(s) 667, 2526
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 3 cut(s) 869, 1292, 2447
BstAUI TGTACA 1 cut(s) 433
BstBAI YACGTR 2 cut(s) 388, 1955
BstC8I GCNNGC 4 cut(s) 276, 640, 1305, 2194
BstDEI CTNAG 8 cut(s) 478, 643, 833, 1037, 1122, 1203, 1281, 1698
BstDSI CCRYGG 2 cut(s) 14, 130
BstEII GGTNACC 1 cut(s) 217
BstF5I GGATG 8 cut(s) 144, 1047, 1476, 1546, 2194, 2377, 2503, 2574
BstFNI CGCG 1 cut(s) 311
BstHHI GCGC 4 cut(s) 95, 806, 1312, 1794
BstMAI GTCTC 9 cut(s) 44, 146, 1038, 1471, 1581, 1824, 1889, 2254, 2528
BstMCI CGRYCG 2 cut(s) 1876, 2618
BstNI CCWGG 9 cut(s) 81, 198, 253, 756, 1067, 1567, 1661, 2280, 2393
BstNSI RCATGY 2 cut(s) 788, 818
BstPAI GACNNNNGTC 1 cut(s) 1483
BstPI GGTNACC 1 cut(s) 217
BstSFI CTRYAG 3 cut(s) 276, 1452, 2148
BstSLI GKGCMC 1 cut(s) 329
BstUI CGCG 1 cut(s) 311
BstV2I GAAGAC 1 cut(s) 1409
BstX2I RGATCY 3 cut(s) 1127, 2135, 2212
BstXI CCANNNNNNTGG 1 cut(s) 2185
BstYI RGATCY 3 cut(s) 1127, 2135, 2212
BsuI GTATCC 4 cut(s) 547, 772, 1150, 1941
BsuRI GGCC 7 cut(s) 135, 183, 274, 454, 465, 1621, 2299
BtgI CCRYGG 2 cut(s) 14, 130
BtsCI GGATG 8 cut(s) 144, 1047, 1476, 1546, 2194, 2377, 2503, 2574
BtsI GCAGTG 1 cut(s) 882
BtsIMutI CAGTG 6 cut(s) 640, 740, 882, 1297, 1916, 2251
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 4 cut(s) 276, 640, 1305, 2194
CaiI CAGNNNCTG 1 cut(s) 1322
CciI TCATGA 1 cut(s) 2356
CfoI GCGC 4 cut(s) 95, 806, 1312, 1794
Cfr13I GGNCC 9 cut(s) 83, 134, 182, 356, 446, 452, 744, 2276, 2297
Cfr9I CCCGGG 1 cut(s) 449
CseI GACGC 4 cut(s) 83, 710, 968, 2570
CsiI ACCWGGT 2 cut(s) 754, 2278
Csp6I GTAC 5 cut(s) 42, 434, 1173, 1209, 1561
CspCI CAANNNNNGTGG 4 cut(s) 151, 186, 1447, 1482
CviQI GTAC 5 cut(s) 42, 434, 1173, 1209, 1561
DdeI CTNAG 8 cut(s) 478, 643, 833, 1037, 1122, 1203, 1281, 1698
DraI TTTAAA 1 cut(s) 415
DraIII CACNNNGTG 2 cut(s) 427, 758
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 2 cut(s) 667, 2526
EarI CTCTTC 2 cut(s) 667, 2526
EciI GGCGGA 1 cut(s) 181
Ecl136II GAGCTC 3 cut(s) 973, 2354, 2578
Eco130I CCWWGG 1 cut(s) 14
Eco147I AGGCCT 2 cut(s) 274, 1621
Eco24I GRGCYC 4 cut(s) 550, 975, 2356, 2580
Eco31I GGTCTC 3 cut(s) 44, 1889, 2254
Eco32I GATATC 2 cut(s) 494, 1978
Eco47I GGWCC 5 cut(s) 83, 356, 446, 744, 2276
Eco53kI GAGCTC 3 cut(s) 973, 2354, 2578
Eco57I CTGAAG 5 cut(s) 1116, 1356, 1715, 1940, 2507
Eco88I CYCGRG 2 cut(s) 47, 449
Eco91I GGTNACC 1 cut(s) 217
EcoICRI GAGCTC 3 cut(s) 973, 2354, 2578
EcoO109I RGGNCCY 3 cut(s) 356, 446, 2276
EcoO65I GGTNACC 1 cut(s) 217
EcoRII CCWGG 9 cut(s) 79, 196, 251, 754, 1065, 1565, 1659, 2278, 2391
EcoRV GATATC 2 cut(s) 494, 1978
EcoT14I CCWWGG 1 cut(s) 14
EcoT22I ATGCAT 3 cut(s) 577, 723, 2121
EcoT38I GRGCYC 4 cut(s) 550, 975, 2356, 2580
ErhI CCWWGG 1 cut(s) 14
Esp3I CGTCTC 1 cut(s) 146
FaqI GGGAC 4 cut(s) 957, 1131, 2409, 2449
FauI CCCGC 2 cut(s) 1819, 2426
FbaI TGATCA 1 cut(s) 2403
FblI GTMKAC 1 cut(s) 2614
FokI GGATG 8 cut(s) 131, 1034, 1483, 1533, 2201, 2384, 2490, 2581
FriOI GRGCYC 4 cut(s) 550, 975, 2356, 2580
FspBI CTAG 5 cut(s) 264, 582, 615, 695, 1055
FspI TGCGCA 1 cut(s) 1311
GlaI GCGC 4 cut(s) 94, 805, 1311, 1793
GsaI CCCAGC 2 cut(s) 216, 366
GsuI CTGGAG 1 cut(s) 912
HaeIII GGCC 7 cut(s) 135, 183, 274, 454, 465, 1621, 2299
HapII CCGG 4 cut(s) 26, 450, 1258, 2531
HgaI GACGC 4 cut(s) 83, 710, 968, 2570
HhaI GCGC 4 cut(s) 95, 806, 1312, 1794
Hin1I GRCGYC 1 cut(s) 75
Hin6I GCGC 4 cut(s) 93, 804, 1310, 1792
HinP1I GCGC 4 cut(s) 93, 804, 1310, 1792
HincII GTYRAC 1 cut(s) 2615
HindII GTYRAC 1 cut(s) 2615
HindIII AAGCTT 1 cut(s) 2165
HinfI GANTC 4 cut(s) 1522, 1664, 2037, 2050
HpaII CCGG 4 cut(s) 26, 450, 1258, 2531
Hpy166II GTNNAC 6 cut(s) 86, 744, 1199, 1563, 1945, 2615
Hpy188III TCNNGA 8 cut(s) 47, 49, 551, 684, 1934, 2357, 2543, 2630
Hpy8I GTNNAC 6 cut(s) 86, 744, 1199, 1563, 1945, 2615
Hpy99I CGWCG 6 cut(s) 71, 74, 77, 116, 984, 2564
HpyAV CCTTC 9 cut(s) 167, 300, 590, 777, 848, 972, 1091, 1341, 1591
HpyCH4III ACNGT 7 cut(s) 505, 1138, 1482, 1726, 1776, 2246, 2400
HpyCH4IV ACGT 3 cut(s) 387, 1838, 1954
HpyF3I CTNAG 8 cut(s) 478, 643, 833, 1037, 1122, 1203, 1281, 1698
HpySE526I ACGT 3 cut(s) 387, 1838, 1954
Hsp92I GRCGYC 1 cut(s) 75
HspAI GCGC 4 cut(s) 93, 804, 1310, 1792
Ksp22I TGATCA 1 cut(s) 2403
LmnI GCTCC 5 cut(s) 34, 970, 1717, 1914, 2575
LweI GCATC 6 cut(s) 210, 529, 1010, 1328, 2179, 2559
MabI ACCWGGT 2 cut(s) 754, 2278
MaeI CTAG 5 cut(s) 264, 582, 615, 695, 1055
MaeII ACGT 3 cut(s) 387, 1838, 1954
MaeIII GTNAC 8 cut(s) 217, 499, 817, 919, 1394, 2287, 2308, 2526
MboII GAAGA 8 cut(s) 684, 1179, 1349, 1409, 1721, 1757, 1933, 2513
MflI RGATCY 3 cut(s) 1127, 2135, 2212
MhlI GDGCHC 7 cut(s) 329, 550, 640, 832, 975, 2356, 2580
MlyI GAGTC 1 cut(s) 2044
MmeI TCCRAC 1 cut(s) 56
Mph1103I ATGCAT 3 cut(s) 577, 723, 2121
MroXI GAANNNNTTC 2 cut(s) 1186, 1655
MseI TTAA 8 cut(s) 398, 414, 1115, 1749, 1787, 1949, 2207, 2498
MslI CAYNNNNRTG 4 cut(s) 237, 726, 2183, 2361
MspA1I CMGCKG 4 cut(s) 317, 510, 998, 1735
MspI CCGG 4 cut(s) 26, 450, 1258, 2531
Mva1269I GAATGC 3 cut(s) 232, 367, 2012
MvaI CCWGG 9 cut(s) 81, 198, 253, 756, 1067, 1567, 1661, 2280, 2393
MvnI CGCG 1 cut(s) 311
NciI CCSGG 4 cut(s) 26, 450, 451, 1259
NcoI CCATGG 1 cut(s) 14
NlaIV GGNNCC 7 cut(s) 30, 136, 160, 184, 448, 746, 2490
NmuCI GTSAC 6 cut(s) 217, 919, 1394, 2287, 2308, 2526
NsbI TGCGCA 1 cut(s) 1311
NsiI ATGCAT 3 cut(s) 577, 723, 2121
NspI RCATGY 2 cut(s) 788, 818
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 1 cut(s) 2356
PaqCI CACCTGC 1 cut(s) 269
PasI CCCWGGG 1 cut(s) 2392
PceI AGGCCT 2 cut(s) 274, 1621
PciI ACATGT 1 cut(s) 814
PctI GAATGC 3 cut(s) 232, 367, 2012
PdmI GAANNNNTTC 2 cut(s) 1186, 1655
PfeI GAWTC 3 cut(s) 1522, 1664, 2037
PflFI GACNNNGTC 1 cut(s) 2280
PflMI CCANNNNNTGG 2 cut(s) 258, 472
PfoI TCCNGGA 2 cut(s) 1257, 1659
Ple19I CGATCG 1 cut(s) 1876
PleI GAGTC 1 cut(s) 2044
PpsI GAGTC 1 cut(s) 2044
Ppu21I YACGTR 2 cut(s) 388, 1955
PpuMI RGGWCCY 3 cut(s) 356, 446, 2276
PscI ACATGT 1 cut(s) 814
PshAI GACNNNNGTC 1 cut(s) 1483
PshBI ATTAAT 1 cut(s) 2498
Psp124BI GAGCTC 3 cut(s) 975, 2356, 2580
Psp5II RGGWCCY 3 cut(s) 356, 446, 2276
Psp6I CCWGG 9 cut(s) 79, 196, 251, 754, 1065, 1565, 1659, 2278, 2391
PspEI GGTNACC 1 cut(s) 217
PspFI CCCAGC 2 cut(s) 212, 362
PspGI CCWGG 9 cut(s) 79, 196, 251, 754, 1065, 1565, 1659, 2278, 2391
PspN4I GGNNCC 7 cut(s) 30, 136, 160, 184, 448, 746, 2490
PspPI GGNCC 9 cut(s) 83, 134, 182, 356, 446, 452, 744, 2276, 2297
PspPPI RGGWCCY 3 cut(s) 356, 446, 2276
PstI CTGCAG 1 cut(s) 280
PstNI CAGNNNCTG 1 cut(s) 1322
PsuI RGATCY 3 cut(s) 1127, 2135, 2212
PsyI GACNNNGTC 1 cut(s) 2280
PvuI CGATCG 1 cut(s) 1876
PvuII CAGCTG 3 cut(s) 317, 998, 1735
RsaI GTAC 5 cut(s) 43, 435, 1174, 1210, 1562
RsaNI GTAC 5 cut(s) 42, 434, 1173, 1209, 1561
RseI CAYNNNNRTG 4 cut(s) 237, 726, 2183, 2361
SacI GAGCTC 3 cut(s) 975, 2356, 2580
SalI GTCGAC 1 cut(s) 2613
SaqAI TTAA 8 cut(s) 398, 414, 1115, 1749, 1787, 1949, 2207, 2498
Sau96I GGNCC 9 cut(s) 83, 134, 182, 356, 446, 452, 744, 2276, 2297
SbfI CCTGCAGG 1 cut(s) 280
ScaI AGTACT 1 cut(s) 1174
SchI GAGTC 1 cut(s) 2044
SdaI CCTGCAGG 1 cut(s) 280
SduI GDGCHC 7 cut(s) 329, 550, 640, 832, 975, 2356, 2580
SexAI ACCWGGT 2 cut(s) 754, 2278
SfaNI GCATC 6 cut(s) 210, 529, 1010, 1328, 2179, 2559
SfcI CTRYAG 3 cut(s) 276, 1452, 2148
Sfr274I CTCGAG 1 cut(s) 47
SinI GGWCC 5 cut(s) 83, 356, 446, 744, 2276
SlaI CTCGAG 1 cut(s) 47
SmaI CCCGGG 1 cut(s) 451
SmiI ATTTAAAT 1 cut(s) 415
SmiMI CAYNNNNRTG 4 cut(s) 237, 726, 2183, 2361
SmlI CTYRAG 3 cut(s) 47, 1225, 2257
SmoI CTYRAG 3 cut(s) 47, 1225, 2257
Sse8387I CCTGCAGG 1 cut(s) 280
SseBI AGGCCT 2 cut(s) 274, 1621
SspMI CTAG 5 cut(s) 264, 582, 615, 695, 1055
SstI GAGCTC 3 cut(s) 975, 2356, 2580
StuI AGGCCT 2 cut(s) 274, 1621
StyI CCWWGG 1 cut(s) 14
SwaI ATTTAAAT 1 cut(s) 415
TaaI ACNGT 7 cut(s) 505, 1138, 1482, 1726, 1776, 2246, 2400
TaiI ACGT 3 cut(s) 390, 1841, 1957
TatI WGTACW 3 cut(s) 433, 1172, 1560
TauI GCSGC 4 cut(s) 119, 314, 351, 791
TfiI GAWTC 3 cut(s) 1522, 1664, 2037
Tru1I TTAA 8 cut(s) 398, 414, 1115, 1749, 1787, 1949, 2207, 2498
Tru9I TTAA 8 cut(s) 398, 414, 1115, 1749, 1787, 1949, 2207, 2498
TscAI CASTG 6 cut(s) 640, 740, 889, 1297, 1923, 2251
TseFI GTSAC 6 cut(s) 217, 919, 1394, 2287, 2308, 2526
Tsp45I GTSAC 6 cut(s) 217, 919, 1394, 2287, 2308, 2526
TspMI CCCGGG 1 cut(s) 449
TspRI CASTG 6 cut(s) 640, 740, 889, 1297, 1923, 2251
Tth111I GACNNNGTC 1 cut(s) 2280
Van91I CCANNNNNTGG 2 cut(s) 258, 472
VpaK11BI GGWCC 5 cut(s) 83, 356, 446, 744, 2276
VspI ATTAAT 1 cut(s) 2498
XapI RAATTY 4 cut(s) 410, 1078, 1240, 2315
XceI RCATGY 2 cut(s) 788, 818
XhoI CTCGAG 1 cut(s) 47
XmaI CCCGGG 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 2614
XmnI GAANNNNTTC 2 cut(s) 1186, 1655
XspI CTAG 5 cut(s) 264, 582, 615, 695, 1055
ZrmI AGTACT 1 cut(s) 1174
Zsp2I ATGCAT 3 cut(s) 577, 723, 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.