pycom12g01190

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004571
Physical Location & Seq
Forward (+)
994263 .. 995466
1204 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGAAAGTGGCTTCTTTGTCCGAAGAACTGTCAGCAGAGAGGGTTCGTATTCTGAGAATAAGTGCAGATTTTGACAATTTCCGGAAAAGGACAGAAAGGGAACGCATTTCTCTCGTAACAAATGCTCAGGGGGAAGTTGTGGAGAGTTTGTTGCCTGTAGTGGATAATTTTGAGAGGGCTAAAACCCAGATTAAGGTGGAGACAGAGGGAGAGGAGAAGATCAACAATAGCTATCAGAGCATTTATAAACAGTTCGGAGAGATTTTAAACTCTCTTGGTGTTGTTCCTGTTGAGACAGTGGGGAAGCCCTTTGATCCATTGTTGCATGAAGCAATTATGCGTGAGGACTCTTCAGAATACGAAGAAGGTGTTATAATCGATGAATTTCGCAAGGGGTTTAAGCTTGGTGACAGGCTTTTGCGTCCGTCAATGGTAAAGGTATCGGCTGGTCCAGGTCCTGCCAAGGTAGAGCAGGAAGTACCTCCATCTGAGGAACAGGATGCAAGTGAAACCGCCGAGAAGGGAACCGAGTCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

180

Amino Acids

20.01

Weight (kDa)

4.77

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 3 - 148 5.6e-41 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 246, 374
AccIII TCCGGA 1 cut(s) 81
AciI CCGC 1 cut(s) 513
AclWI GGATC 1 cut(s) 308
AcsI RAATTY 1 cut(s) 383
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 1 cut(s) 480
AfiI CCNNNNNNNGG 1 cut(s) 193
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 3 cut(s) 231, 403, 536
AluI AGCT 3 cut(s) 231, 403, 536
Alw26I GTCTC 2 cut(s) 194, 287
AlwI GGATC 1 cut(s) 308
AlwNI CAGNNNCTG 1 cut(s) 458
Aor13HI TCCGGA 1 cut(s) 81
ApoI RAATTY 1 cut(s) 383
AspS9I GGNCC 2 cut(s) 449, 455
AsuHPI GGTGA 1 cut(s) 419
AvaII GGWCC 2 cut(s) 449, 455
BccI CCATC 1 cut(s) 493
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 1 cut(s) 453
BcoDI GTCTC 2 cut(s) 194, 287
BfmI CTRYAG 1 cut(s) 156
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 2 cut(s) 449, 455
BmgT120I GGNCC 2 cut(s) 449, 455
BmiI GGNNCC 1 cut(s) 526
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 1 cut(s) 490
Bpu10I CCTNAGC 1 cut(s) 126
Bsa29I ATCGAT 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 462
BsaWI WCCGGW 1 cut(s) 81
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 193
BseAI TCCGGA 1 cut(s) 81
BseBI CCWGG 1 cut(s) 453
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 462
BseGI GGATG 1 cut(s) 505
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 3 cut(s) 44, 140, 480
BseRI GAGGAG 1 cut(s) 227
BsgI GTGCAG 1 cut(s) 84
BshVI ATCGAT 1 cut(s) 378
BsiSI CCGG 1 cut(s) 82
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 2 cut(s) 194, 287
Bsp13I TCCGGA 1 cut(s) 81
Bsp143I GATC 2 cut(s) 219, 313
BspACI CCGC 1 cut(s) 513
BspCNI CTCAG 3 cut(s) 45, 139, 481
BspDI ATCGAT 1 cut(s) 378
BspEI TCCGGA 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 526
BspPI GGATC 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 462
BssMI GATC 2 cut(s) 219, 313
BssT1I CCWWGG 1 cut(s) 462
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 3 cut(s) 30, 252, 298
Bst6I CTCTTC 1 cut(s) 355
BstDEI CTNAG 3 cut(s) 53, 126, 489
BstF5I GGATG 1 cut(s) 505
BstKTI GATC 2 cut(s) 222, 316
BstMAI GTCTC 2 cut(s) 194, 287
BstMBI GATC 2 cut(s) 219, 313
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 156
Bsu15I ATCGAT 1 cut(s) 378
BsuTUI ATCGAT 1 cut(s) 378
BtsCI GGATG 1 cut(s) 505
BtsIMutI CAGTG 1 cut(s) 303
CaiI CAGNNNCTG 1 cut(s) 458
Cfr13I GGNCC 2 cut(s) 449, 455
ClaI ATCGAT 1 cut(s) 378
CseI GACGC 1 cut(s) 410
Csp6I GTAC 1 cut(s) 479
CviAII CATG 1 cut(s) 326
CviJI RGCY 8 cut(s) 11, 179, 231, 307, 403, 415, 446, 536
CviKI_1 RGCY 8 cut(s) 11, 179, 231, 307, 403, 415, 446, 536
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 3 cut(s) 53, 126, 489
DpnI GATC 2 cut(s) 221, 315
DpnII GATC 2 cut(s) 219, 313
DraI TTTAAA 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 355
Eco130I CCWWGG 1 cut(s) 462
Eco47I GGWCC 2 cut(s) 449, 455
Eco57I CTGAAG 1 cut(s) 336
EcoO109I RGGNCCY 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 462
FaeI CATG 1 cut(s) 329
FaiI YATR 4 cut(s) 246, 327, 338, 374
FatI CATG 1 cut(s) 325
FokI GGATG 1 cut(s) 512
HapII CCGG 1 cut(s) 82
HgaI GACGC 1 cut(s) 410
Hin1II CATG 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 2 cut(s) 347, 530
HpaII CCGG 1 cut(s) 82
HphI GGTGA 1 cut(s) 419
Hpy188I TCNGA 6 cut(s) 22, 54, 237, 257, 355, 490
Hpy188III TCNNGA 1 cut(s) 82
HpyAV CCTTC 2 cut(s) 359, 514
HpyCH4III ACNGT 3 cut(s) 30, 252, 298
HpyCH4V TGCA 3 cut(s) 65, 325, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpyF3I CTNAG 3 cut(s) 53, 126, 489
Hsp92II CATG 1 cut(s) 329
Kpn2I TCCGGA 1 cut(s) 81
Kzo9I GATC 2 cut(s) 219, 313
LweI GCATC 1 cut(s) 490
MaeIII GTNAC 2 cut(s) 115, 407
MalI GATC 2 cut(s) 221, 315
MboI GATC 2 cut(s) 219, 313
MboII GAAGA 4 cut(s) 35, 229, 342, 374
MluCI AATT 4 cut(s) 76, 166, 333, 383
MlyI GAGTC 2 cut(s) 341, 539
MnlI CCTC 7 cut(s) 33, 168, 199, 205, 337, 484, 492
MroI TCCGGA 1 cut(s) 81
MseI TTAA 4 cut(s) 192, 266, 399, 538
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 453
MwoI GCNNNNNNNGC 1 cut(s) 237
NdeII GATC 2 cut(s) 219, 313
NlaIII CATG 1 cut(s) 329
NlaIV GGNNCC 1 cut(s) 526
NmuCI GTSAC 1 cut(s) 407
PleI GAGTC 2 cut(s) 341, 538
PpsI GAGTC 2 cut(s) 341, 538
PpuMI RGGWCCY 1 cut(s) 455
PsiI TTATAA 2 cut(s) 246, 374
Psp5II RGGWCCY 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 526
PspPI GGNCC 2 cut(s) 449, 455
PspPPI RGGWCCY 1 cut(s) 455
PstNI CAGNNNCTG 1 cut(s) 458
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
SaqAI TTAA 4 cut(s) 192, 266, 399, 538
Sau3AI GATC 2 cut(s) 219, 313
Sau96I GGNCC 2 cut(s) 449, 455
SchI GAGTC 2 cut(s) 341, 539
ScrFI CCNGG 1 cut(s) 453
SetI ASST 9 cut(s) 198, 233, 370, 405, 441, 457, 468, 484, 538
SfaNI GCATC 1 cut(s) 490
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 2 cut(s) 449, 455
Sse9I AATT 4 cut(s) 76, 166, 333, 383
SsiI CCGC 1 cut(s) 513
StyD4I CCNGG 1 cut(s) 451
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 3 cut(s) 30, 252, 298
TaqI TCGA 1 cut(s) 378
TasI AATT 4 cut(s) 76, 166, 333, 383
Tru1I TTAA 4 cut(s) 192, 266, 399, 538
Tru9I TTAA 4 cut(s) 192, 266, 399, 538
TscAI CASTG 1 cut(s) 303
TseFI GTSAC 1 cut(s) 407
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 3 cut(s) 17, 342, 396
TspGWI ACGGA 1 cut(s) 414
TspRI CASTG 1 cut(s) 303
VpaK11BI GGWCC 2 cut(s) 449, 455
XapI RAATTY 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.