pycom12g02410

Heme-binding-like protein At3g10130

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008357
Physical Location & Seq
Forward (+)
2080380 .. 2082744
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
ATGAACCACTACTCCCTCTCTCACTTCCTCTCTGCAACAGAAAATTCAAAAAAATCAAGAAAAAAGCTTTCAATGTCGTTCACTCACACGCACCCTTCCAAAACCCTAACCTGCACTTCCCTCTCCGGAAACCCTAACATCTACAGACGACTCACCACCATCTCCATGGCGACGGACCGAGCGGCCGCAGTCCCCTCCCCGCCGCAGCAGCGGAGGAGGACCATGTCCGCTCTAGAAGCCCGAGTCTCCCTCGTCGCCGCTCTCGCTATTCAAGCGTCGTCTCTCTCCCAGCGACTTTTGCTCGAGTTGGCCACGGAGACTGCAAAATATGTCTTCCCCAAACAGTTTGAGGCTCGTACTCTCGAAGAAGCCCTCATGGCAGTTCCGGACATTGAAACGGTGAAGTTCAAAGTTCTGAGTAGGAGAGACCAGTATGAGATTAGAGAAATTGAGGCTTACATTATAGCAGAGACAACAATGCCTGGAAAGACTGGTTTTGATTTCAATGGTGCATCTCAATCCTTCAATGTCTTAGCTGAATACCTGTTTGGTAAGAATACCACCAAGGAGAAAATGGAGATGACCACACCGGTTTATACACGTAAAGTTCAATCGGATGGGGAGAAAATGGAAATGACAACTCCTGTGATAACAAAGAGGCTGGGAGATCAAGATAAATGGCAGATGTCCTTTGTCATTCCCTCGAAATACGGTGCCAATACGCCATTGCCTAAAGATCCATCTGTAAGGATCGAAGAGGTCCCTAGGAAAGTAGTTGCAGTTGTTGCCTTTTCAGGGTTTGTTACTAATGAGGAAGTTAAGAAACGAGAATCCAAGCTAAGGGAAGCTCTGAAAAATGATGGACAGTTCCAAGTGAAGGAAGGAACTTCAGTGGAAGTTGCACAGTATAATCCACCATTTACACTTCCATTTCAACGGCGTAATGAGATCAGTCTGGAAGTTGAAAGTAAAGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

327

Amino Acids

36.84

Weight (kDa)

9.34

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 131 - 319 8.5e-47 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 713
AccBSI CCGCTC 3 cut(s) 182, 230, 260
AccIII TCCGGA 2 cut(s) 125, 385
AciI CCGC 7 cut(s) 182, 186, 200, 203, 211, 228, 258
AclWI GGATC 2 cut(s) 731, 758
AcoI YGGCCR 2 cut(s) 183, 309
AcsI RAATTY 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 873
AfaI GTAC 1 cut(s) 358
AfiI CCNNNNNNNGG 3 cut(s) 795, 840, 877
AflIII ACRYGT 1 cut(s) 599
AgeI ACCGGT 1 cut(s) 589
AjnI CCWGG 1 cut(s) 481
AjuI GAANNNNNNNTTGG 2 cut(s) 531, 563
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 67, 536, 838, 848
AluI AGCT 4 cut(s) 67, 536, 838, 848
Alw26I GTCTC 5 cut(s) 250, 285, 311, 420, 464
AlwI GGATC 2 cut(s) 731, 758
Ama87I CYCGRG 2 cut(s) 240, 302
Aor13HI TCCGGA 2 cut(s) 125, 385
AoxI GGCC 2 cut(s) 183, 309
ApeKI GCWGC 2 cut(s) 205, 208
ApoI RAATTY 1 cut(s) 43
AsiGI ACCGGT 1 cut(s) 589
AspA2I CCTAGG 1 cut(s) 764
AspS9I GGNCC 3 cut(s) 175, 219, 760
AsuHPI GGTGA 2 cut(s) 145, 412
AvaI CYCGRG 2 cut(s) 240, 302
AvaII GGWCC 3 cut(s) 175, 219, 760
AvrII CCTAGG 1 cut(s) 764
BalI TGGCCA 1 cut(s) 311
BanI GGYRCC 1 cut(s) 713
BbsI GAAGAC 1 cut(s) 325
BbvI GCAGC 2 cut(s) 217, 220
BccI CCATC 4 cut(s) 167, 611, 748, 854
BceAI ACGGC 1 cut(s) 953
BciT130I CCWGG 1 cut(s) 483
BcoDI GTCTC 5 cut(s) 250, 285, 311, 420, 464
BfaI CTAG 2 cut(s) 233, 765
BfmI CTRYAG 1 cut(s) 142
BfuAI ACCTGC 1 cut(s) 119
BglI GCCNNNNNGGC 1 cut(s) 377
BisI GCNGC 6 cut(s) 183, 186, 203, 206, 209, 258
BlnI CCTAGG 1 cut(s) 764
BlsI GCNGC 6 cut(s) 184, 187, 204, 207, 210, 259
Bme1390I CCNGG 1 cut(s) 483
Bme18I GGWCC 3 cut(s) 175, 219, 760
BmeT110I CYCGRG 2 cut(s) 240, 302
BmgT120I GGNCC 3 cut(s) 175, 219, 760
BmiI GGNNCC 2 cut(s) 715, 762
BmrFI CCNGG 1 cut(s) 483
BmsI GCATC 1 cut(s) 521
BpiI GAAGAC 1 cut(s) 325
BplI GAGNNNNNCTC 2 cut(s) 234, 266
Bpu10I CCTNAGC 1 cut(s) 839
BsaAI YACGTR 1 cut(s) 602
BsaI GGTCTC 1 cut(s) 420
BsaJI CCNNGG 4 cut(s) 165, 312, 564, 764
BsaWI WCCGGW 3 cut(s) 125, 385, 589
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 795, 840, 877
Bse118I RCCGGY 1 cut(s) 589
Bse1I ACTGG 2 cut(s) 430, 496
Bse3DI GCAATG 1 cut(s) 725
BseAI TCCGGA 2 cut(s) 125, 385
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 4 cut(s) 165, 312, 564, 764
BseGI GGATG 1 cut(s) 622
BseLI CCNNNNNNNGG 3 cut(s) 795, 840, 877
BseMI GCAATG 1 cut(s) 725
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 2 cut(s) 430, 496
BseRI GAGGAG 1 cut(s) 229
BseX3I CGGCCG 1 cut(s) 183
BseXI GCAGC 2 cut(s) 217, 220
BseYI CCCAGC 2 cut(s) 288, 661
BsgI GTGCAG 1 cut(s) 97
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 2 cut(s) 185, 311
BshNI GGYRCC 1 cut(s) 713
BshTI ACCGGT 1 cut(s) 589
BsiEI CGRYCG 1 cut(s) 186
BsiHKCI CYCGRG 2 cut(s) 240, 302
BsiSI CCGG 3 cut(s) 126, 386, 590
BslFI GGGAC 2 cut(s) 176, 746
BslI CCNNNNNNNGG 3 cut(s) 795, 840, 877
BsmAI GTCTC 5 cut(s) 250, 285, 311, 420, 464
BsmBI CGTCTC 1 cut(s) 285
BsmFI GGGAC 2 cut(s) 176, 746
BsnI GGCC 2 cut(s) 185, 311
Bso31I GGTCTC 1 cut(s) 420
BsoBI CYCGRG 2 cut(s) 240, 302
Bsp13I TCCGGA 2 cut(s) 125, 385
Bsp143I GATC 4 cut(s) 667, 736, 750, 948
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 7 cut(s) 182, 186, 200, 203, 211, 228, 258
BspANI GGCC 2 cut(s) 185, 311
BspCNI CTCAG 1 cut(s) 408
BspEI TCCGGA 2 cut(s) 125, 385
BspLI GGNNCC 2 cut(s) 715, 762
BspMI ACCTGC 1 cut(s) 119
BspPI GGATC 2 cut(s) 731, 758
BspT107I GGYRCC 1 cut(s) 713
BspTNI GGTCTC 1 cut(s) 420
BsrBI CCGCTC 3 cut(s) 182, 230, 260
BsrDI GCAATG 1 cut(s) 725
BsrFI RCCGGY 1 cut(s) 589
BsrI ACTGG 2 cut(s) 430, 496
BssAI RCCGGY 1 cut(s) 589
BssECI CCNNGG 4 cut(s) 165, 312, 564, 764
BssMI GATC 4 cut(s) 667, 736, 750, 948
BssT1I CCWWGG 3 cut(s) 165, 564, 764
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 5 cut(s) 345, 400, 713, 867, 906
Bst6I CTCTTC 1 cut(s) 750
BstAPI GCANNNNNTGC 1 cut(s) 785
BstBAI YACGTR 1 cut(s) 602
BstDEI CTNAG 3 cut(s) 416, 532, 839
BstDSI CCRYGG 2 cut(s) 165, 312
BstF5I GGATG 1 cut(s) 622
BstKTI GATC 4 cut(s) 670, 739, 753, 951
BstMAI GTCTC 5 cut(s) 250, 285, 311, 420, 464
BstMBI GATC 4 cut(s) 667, 736, 750, 948
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 7 cut(s) 208, 236, 263, 272, 298, 377, 785
BstNI CCWGG 1 cut(s) 483
BstSCI CCNGG 1 cut(s) 481
BstSFI CTRYAG 1 cut(s) 142
BstV1I GCAGC 2 cut(s) 217, 220
BstV2I GAAGAC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 736
BstXI CCANNNNNNTGG 1 cut(s) 166
BstYI RGATCY 1 cut(s) 736
BstZI CGGCCG 1 cut(s) 183
BsuRI GGCC 2 cut(s) 185, 311
BtgI CCRYGG 2 cut(s) 165, 312
BtsCI GGATG 1 cut(s) 622
BtsIMutI CAGTG 1 cut(s) 897
BveI ACCTGC 1 cut(s) 119
CciNI GCGGCCGC 1 cut(s) 183
Cfr10I RCCGGY 1 cut(s) 589
Cfr13I GGNCC 3 cut(s) 175, 219, 760
CpoI CGGWCCG 1 cut(s) 175
CseI GACGC 1 cut(s) 264
Csp6I GTAC 1 cut(s) 357
CspAI ACCGGT 1 cut(s) 589
CspI CGGWCCG 1 cut(s) 175
CviAII CATG 3 cut(s) 166, 223, 376
CviQI GTAC 1 cut(s) 357
DdeI CTNAG 3 cut(s) 416, 532, 839
DpnI GATC 4 cut(s) 669, 738, 752, 950
DpnII GATC 4 cut(s) 667, 736, 750, 948
EaeI YGGCCR 2 cut(s) 183, 309
EagI CGGCCG 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 750
EarI CTCTTC 1 cut(s) 750
EclXI CGGCCG 1 cut(s) 183
Eco130I CCWWGG 3 cut(s) 165, 564, 764
Eco31I GGTCTC 1 cut(s) 420
Eco47I GGWCC 3 cut(s) 175, 219, 760
Eco52I CGGCCG 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 873
Eco88I CYCGRG 2 cut(s) 240, 302
EcoO109I RGGNCCY 1 cut(s) 760
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 3 cut(s) 165, 564, 764
ErhI CCWWGG 3 cut(s) 165, 564, 764
Esp3I CGTCTC 1 cut(s) 285
FaeI CATG 3 cut(s) 169, 226, 379
FaiI YATR 8 cut(s) 167, 224, 330, 377, 435, 464, 597, 909
FaqI GGGAC 2 cut(s) 176, 746
FatI CATG 3 cut(s) 165, 222, 375
FauI CCCGC 1 cut(s) 207
Fnu4HI GCNGC 6 cut(s) 183, 186, 203, 206, 209, 258
FokI GGATG 1 cut(s) 629
Fsp4HI GCNGC 6 cut(s) 183, 186, 203, 206, 209, 258
FspBI CTAG 2 cut(s) 233, 765
GluI GCNGC 6 cut(s) 183, 186, 203, 206, 209, 258
GsaI CCCAGC 2 cut(s) 292, 665
HaeIII GGCC 2 cut(s) 185, 311
HapII CCGG 3 cut(s) 126, 386, 590
HgaI GACGC 1 cut(s) 264
Hin1II CATG 3 cut(s) 169, 226, 379
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 3 cut(s) 150, 243, 830
HpaII CCGG 3 cut(s) 126, 386, 590
HphI GGTGA 2 cut(s) 145, 412
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 3 cut(s) 417, 616, 852
Hpy188III TCNNGA 7 cut(s) 57, 126, 233, 362, 386, 671, 956
Hpy8I GTNNAC 1 cut(s) 81
Hpy99I CGWCG 3 cut(s) 175, 257, 280
HpyAV CCTTC 4 cut(s) 105, 532, 871, 875
HpyCH4III ACNGT 5 cut(s) 345, 400, 713, 867, 906
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 6 cut(s) 35, 114, 323, 512, 779, 902
HpyF10VI GCNNNNNNNGC 7 cut(s) 208, 236, 263, 272, 298, 377, 785
HpyF3I CTNAG 3 cut(s) 416, 532, 839
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 3 cut(s) 169, 226, 379
Kpn2I TCCGGA 2 cut(s) 125, 385
Kzo9I GATC 4 cut(s) 667, 736, 750, 948
Lsp1109I GCAGC 2 cut(s) 217, 220
LweI GCATC 1 cut(s) 521
MaeI CTAG 2 cut(s) 233, 765
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 1 cut(s) 802
MalI GATC 4 cut(s) 669, 738, 752, 950
MbiI CCGCTC 3 cut(s) 182, 230, 260
MboI GATC 4 cut(s) 667, 736, 750, 948
MboII GAAGA 3 cut(s) 325, 377, 767
MflI RGATCY 1 cut(s) 736
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 2 cut(s) 43, 447
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 2 cut(s) 144, 252
Mox20I TGGCCA 1 cut(s) 311
MroI TCCGGA 2 cut(s) 125, 385
MscI TGGCCA 1 cut(s) 311
MseI TTAA 1 cut(s) 819
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 211
MspI CCGG 3 cut(s) 126, 386, 590
MspR9I CCNGG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 7 cut(s) 208, 236, 263, 272, 298, 377, 785
NcoI CCATGG 1 cut(s) 165
NdeII GATC 4 cut(s) 667, 736, 750, 948
NlaIII CATG 3 cut(s) 169, 226, 379
NlaIV GGNNCC 2 cut(s) 715, 762
NotI GCGGCCGC 1 cut(s) 183
PaeR7I CTCGAG 1 cut(s) 302
PfeI GAWTC 1 cut(s) 830
PflFI GACNNNGTC 1 cut(s) 223
PinAI ACCGGT 1 cut(s) 589
PkrI GCNGC 6 cut(s) 184, 187, 204, 207, 210, 259
PleI GAGTC 2 cut(s) 144, 251
PpsI GAGTC 2 cut(s) 144, 251
Ppu21I YACGTR 1 cut(s) 602
PpuMI RGGWCCY 1 cut(s) 760
Psp5II RGGWCCY 1 cut(s) 760
Psp6I CCWGG 1 cut(s) 481
PspFI CCCAGC 2 cut(s) 288, 661
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 2 cut(s) 715, 762
PspPI GGNCC 3 cut(s) 175, 219, 760
PspPPI RGGWCCY 1 cut(s) 760
PspXI VCTCGAGB 1 cut(s) 302
PsuI RGATCY 1 cut(s) 736
PsyI GACNNNGTC 1 cut(s) 223
RsaI GTAC 1 cut(s) 358
RsaNI GTAC 1 cut(s) 357
RseI CAYNNNNRTG 1 cut(s) 164
Rsr2I CGGWCCG 1 cut(s) 175
RsrII CGGWCCG 1 cut(s) 175
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 6 cut(s) 183, 186, 203, 206, 209, 258
Sau3AI GATC 4 cut(s) 667, 736, 750, 948
Sau96I GGNCC 3 cut(s) 175, 219, 760
SchI GAGTC 2 cut(s) 144, 252
ScrFI CCNGG 1 cut(s) 483
SetI ASST 8 cut(s) 69, 113, 538, 546, 604, 762, 840, 850
SfaNI GCATC 1 cut(s) 521
SfcI CTRYAG 1 cut(s) 142
Sfr274I CTCGAG 1 cut(s) 302
SinI GGWCC 3 cut(s) 175, 219, 760
SlaI CTCGAG 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 2 cut(s) 43, 447
SsiI CCGC 7 cut(s) 182, 186, 200, 203, 211, 228, 258
SspMI CTAG 2 cut(s) 233, 765
StyD4I CCNGG 1 cut(s) 481
StyI CCWWGG 3 cut(s) 165, 564, 764
TaaI ACNGT 5 cut(s) 345, 400, 713, 867, 906
TaiI ACGT 1 cut(s) 604
TaqI TCGA 4 cut(s) 303, 363, 704, 753
TaqII GACCGA 1 cut(s) 192
TasI AATT 2 cut(s) 43, 447
TauI GCSGC 4 cut(s) 185, 188, 205, 260
TfiI GAWTC 1 cut(s) 830
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TscAI CASTG 1 cut(s) 897
TseI GCWGC 2 cut(s) 205, 208
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 188, 329
TspRI CASTG 1 cut(s) 897
Tth111I GACNNNGTC 1 cut(s) 223
VpaK11BI GGWCC 3 cut(s) 175, 219, 760
XapI RAATTY 1 cut(s) 43
XbaI TCTAGA 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 2 cut(s) 163, 571
XhoI CTCGAG 1 cut(s) 302
XmaJI CCTAGG 1 cut(s) 764
XspI CTAG 2 cut(s) 233, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.