pycom12g03830

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006819
Physical Location & Seq
Forward (+)
3698415 .. 3700859
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1401 bp
ATGGAGTCTGAAAATGGGGTTACATTCGACGATGAGAGTTGTGTTATCGAGGAAAAACATGTAGATGTATCAGTACCGAGTTTGAGCAAAGAGGGAAAGAATGCTGATGGTAATGCAGAAGTTCCTGCCGTGAATGGAATATCTGAACCTGTGACAAAAGATGAAGGCATTAACTCTTCTGAAGTTGCAGTTGCAGCCTCTGCGACTGCTCCACCTGGTAAAAATTCAAAAGCTGCAAAGAATCCTCATGCTCCGAACAATGATTTTTCAAAGAGCAAACCGGCCAAGGATAAACCTACTGTGAAAGGCGCAACTCCATTCCCTCATAAGGAGAGGGCATTAATTTCTCAGAGTCTTTCTTTCCCAGCAAGAGGATCTCGTGTGGATCCTATGAAGAAGAGTATTGATGTGTACCCGGTGAAAACAGAAGTCAAACATGCTCGAGGAGCAACCAAAGCTGAGGCTCCCATTTTGGTTTCTCGTCTGAATAATCCAACTAGGCGGGCATCCACAGGAGTTCATTCAAAGGATGGGAACACAACTGGAGCTTCCATTAAGAGGACATCTTTAGCATCAATACCCAGCATTAGATCCTCTTCATCCGGCAAGTCTGGTTCTGTGGATACATCCGCCAATAACCCATCTAATGGGATCCGATCAGTTGATCAAAGTTTAAATGCTGTAAAAACAACTCTGCCAATCAAAGAGGATGATGATGCTCACTCCGTTGCTTCGTTGGAAGAACGTGCTGAAAAACGGAAGGAGTTTTTTACGAAGCTGGAAGAAAAGATACAGGCTAAGGAAGAAGAAAAAAATAACTCGCAAGCAAAATCAAAGGAAAGCCAGGAAGCTGAGATCAAGCGACTAAGGAAGAGCCTGACGTTTAAGGCGGCGCCCATGCCAAGTTTCTATAAAGAGCCTCCTCCGAAAGTTGAACTCAAGAAGATTCCAACCACACGGCCAAAATCTCCAAAACTTGGACGGAACAAGAGCTCCATTTCTTCGCTAAACAGTTCTTCAGAAGGTGCTGGGGCATCTCTTAGCCCACGTCTGAATCAAGAACTGAATAATTCAAAGAAAGGATTACAGACTAAAAGCGAAAAGGATGTCATAGATCCAAATAAGCCTATTAAAAAGTCTCAAACTAGGCTTCATTCTCAGGAAAATGCTGCTACTAAAACCGAAGCAAAGCCTGCAAAGTCACCACCAAAGGGTATCAAAGAAACAAAAGACCAGAAAGCGCACACCGGAAAAACTGAAGAAGCTCAGGACCAGTCAGGGCATCTTTCCGAATTCAAAGATGAGATAGAGCCTGAAGTGAGTGTTGGCCAGATCAAAGAACCAGTCCTCGGTGCACCTATCCCAGAGATCATGCCTCATGAAGTTTCGGTTGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

467

Amino Acids

50.02

Weight (kDa)

9.36

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 247 - 316 4.3e-23 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 892
AccB7I CCANNNNNTGG 2 cut(s) 647, 977
AciI CCGC 3 cut(s) 502, 630, 890
AclWI GGATC 7 cut(s) 380, 382, 393, 585, 646, 659, 1109
AcoI YGGCCR 3 cut(s) 282, 959, 1327
AcsI RAATTY 2 cut(s) 223, 1292
AcuI CTGAAG 4 cut(s) 201, 1002, 1278, 1335
AcyI GRCGYC 1 cut(s) 893
AfaI GTAC 2 cut(s) 75, 413
AfiI CCNNNNNNNGG 7 cut(s) 328, 371, 558, 647, 977, 1211, 1349
AflIII ACRYGT 1 cut(s) 58
AgsI TTSAA 6 cut(s) 228, 270, 525, 935, 1074, 1297
AjiI CACGTC 1 cut(s) 1049
AjnI CCWGG 2 cut(s) 214, 843
AjuI GAANNNNNNNTTGG 3 cut(s) 1308, 1340, 1374
AloI GAACNNNNNNTCC 2 cut(s) 977, 1009
AluBI AGCT 7 cut(s) 233, 458, 548, 778, 851, 993, 1265
AluI AGCT 7 cut(s) 233, 458, 548, 778, 851, 993, 1265
Alw21I GWGCWC 2 cut(s) 995, 1357
Alw26I GTCTC 1 cut(s) 1143
Alw44I GTGCAC 1 cut(s) 1353
AlwI GGATC 7 cut(s) 380, 382, 393, 585, 646, 659, 1109
AlwNI CAGNNNCTG 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 441
AoxI GGCC 3 cut(s) 282, 959, 1327
ApaLI GTGCAC 1 cut(s) 1353
ApeKI GCWGC 3 cut(s) 194, 233, 1169
ApoI RAATTY 2 cut(s) 223, 1292
ArsI GACNNNNNNTTYG 4 cut(s) 1092, 1124, 1329, 1361
AseI ATTAAT 1 cut(s) 341
AspLEI GCGC 3 cut(s) 311, 895, 1243
AspS9I GGNCC 1 cut(s) 1270
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 2 cut(s) 430, 1194
AvaI CYCGRG 1 cut(s) 441
AvaII GGWCC 1 cut(s) 1270
BaeGI GKGCMC 1 cut(s) 1357
BalI TGGCCA 1 cut(s) 1329
BamHI GGATCC 2 cut(s) 385, 651
BanI GGYRCC 1 cut(s) 892
BanII GRGCYC 1 cut(s) 995
BarI GAAGNNNNNNTAC 2 cut(s) 774, 806
BauI CACGAG 1 cut(s) 378
Bbv12I GWGCWC 2 cut(s) 995, 1357
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 3 cut(s) 206, 220, 1156
BccI CCATC 3 cut(s) 101, 524, 649
BceAI ACGGC 2 cut(s) 113, 974
BciT130I CCWGG 2 cut(s) 216, 845
BciVI GTATCC 1 cut(s) 616
BclI TGATCA 1 cut(s) 664
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 1143
BfaI CTAG 2 cut(s) 498, 1146
BfoI RGCGCY 1 cut(s) 896
BfuI GTATCC 1 cut(s) 616
BisI GCNGC 4 cut(s) 195, 234, 891, 1170
BlsI GCNGC 4 cut(s) 196, 235, 892, 1171
Bme1390I CCNGG 3 cut(s) 216, 416, 845
Bme18I GGWCC 1 cut(s) 1270
BmeT110I CYCGRG 1 cut(s) 441
BmgBI CACGTC 1 cut(s) 1049
BmgT120I GGNCC 1 cut(s) 1270
BmiI GGNNCC 4 cut(s) 387, 465, 653, 894
BmrFI CCNGG 3 cut(s) 216, 416, 845
BmsI GCATC 5 cut(s) 515, 581, 706, 1043, 1291
BpmI CTGGAG 1 cut(s) 564
Bpu10I CCTNAGC 3 cut(s) 459, 798, 1266
BpuEI CTTGAG 1 cut(s) 923
BpuMI CCSGG 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 893
BsaJI CCNNGG 2 cut(s) 285, 1348
BsaWI WCCGGW 1 cut(s) 1247
BsaXI ACNNNNNCTCC 2 cut(s) 977, 1007
Bsc4I CCNNNNNNNGG 7 cut(s) 328, 371, 558, 647, 977, 1211, 1349
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 3 cut(s) 547, 1273, 1343
BseBI CCWGG 2 cut(s) 216, 845
BseDI CCNNGG 2 cut(s) 285, 1348
BseGI GGATG 6 cut(s) 506, 535, 599, 626, 715, 1111
BseLI CCNNNNNNNGG 7 cut(s) 328, 371, 558, 647, 977, 1211, 1349
BseMII CTCAG 5 cut(s) 362, 450, 843, 1172, 1280
BseNI ACTGG 3 cut(s) 547, 1273, 1343
BseRI GAGGAG 2 cut(s) 459, 912
BseSI GKGCMC 1 cut(s) 1357
BseXI GCAGC 3 cut(s) 206, 220, 1156
BseYI CCCAGC 3 cut(s) 364, 581, 1028
BshFI GGCC 3 cut(s) 284, 961, 1329
BshNI GGYRCC 1 cut(s) 892
BsiHKAI GWGCWC 2 cut(s) 995, 1357
BsiHKCI CYCGRG 1 cut(s) 441
BsiSI CCGG 4 cut(s) 281, 416, 603, 1248
BslI CCNNNNNNNGG 7 cut(s) 328, 371, 558, 647, 977, 1211, 1349
BsmAI GTCTC 1 cut(s) 1143
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 3 cut(s) 284, 961, 1329
BsoBI CYCGRG 1 cut(s) 441
Bsp1286I GDGCHC 2 cut(s) 995, 1357
BspACI CCGC 3 cut(s) 502, 630, 890
BspANI GGCC 3 cut(s) 284, 961, 1329
BspCNI CTCAG 5 cut(s) 361, 451, 844, 1171, 1279
BspHI TCATGA 1 cut(s) 1378
BspLI GGNNCC 4 cut(s) 387, 465, 653, 894
BspPI GGATC 7 cut(s) 380, 382, 393, 585, 646, 659, 1109
BspQI GCTCTTC 1 cut(s) 866
BspT107I GGYRCC 1 cut(s) 892
BsrFI RCCGGY 1 cut(s) 280
BsrI ACTGG 3 cut(s) 547, 1273, 1343
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 2 cut(s) 285, 1348
BssNI GRCGYC 1 cut(s) 893
BssSI CACGAG 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 378
Bst2UI CCWGG 2 cut(s) 216, 845
Bst4CI ACNGT 2 cut(s) 301, 1013
Bst6I CTCTTC 4 cut(s) 181, 392, 601, 866
BstACI GRCGYC 1 cut(s) 893
BstAPI GCANNNNNTGC 2 cut(s) 200, 1193
BstC8I GCNNGC 3 cut(s) 504, 825, 1194
BstDEI CTNAG 8 cut(s) 348, 459, 798, 852, 866, 1040, 1158, 1266
BstF5I GGATG 6 cut(s) 506, 535, 599, 626, 715, 1111
BstH2I RGCGCY 1 cut(s) 896
BstHHI GCGC 3 cut(s) 311, 895, 1243
BstMAI GTCTC 1 cut(s) 1143
BstMWI GCNNNNNNNGC 5 cut(s) 194, 200, 446, 455, 1193
BstNI CCWGG 2 cut(s) 216, 845
BstNSI RCATGY 2 cut(s) 62, 440
BstSCI CCNGG 3 cut(s) 214, 414, 843
BstSLI GKGCMC 1 cut(s) 1357
BstV1I GCAGC 3 cut(s) 206, 220, 1156
BstX2I RGATCY 5 cut(s) 374, 385, 590, 651, 1114
BstYI RGATCY 5 cut(s) 374, 385, 590, 651, 1114
BsuI GTATCC 1 cut(s) 616
BsuRI GGCC 3 cut(s) 284, 961, 1329
BtrI CACGTC 1 cut(s) 1049
BtsCI GGATG 6 cut(s) 506, 535, 599, 626, 715, 1111
Cac8I GCNNGC 3 cut(s) 504, 825, 1194
CaiI CAGNNNCTG 1 cut(s) 200
CciI TCATGA 1 cut(s) 1378
CfoI GCGC 3 cut(s) 311, 895, 1243
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 1 cut(s) 1270
CsiI ACCWGGT 1 cut(s) 214
Csp6I GTAC 2 cut(s) 74, 412
CviAII CATG 6 cut(s) 59, 248, 437, 898, 1372, 1379
CviQI GTAC 2 cut(s) 74, 412
DdeI CTNAG 8 cut(s) 348, 459, 798, 852, 866, 1040, 1158, 1266
DinI GGCGCC 1 cut(s) 894
DraI TTTAAA 1 cut(s) 675
EaeI YGGCCR 3 cut(s) 282, 959, 1327
Eam1104I CTCTTC 4 cut(s) 181, 392, 601, 866
EarI CTCTTC 4 cut(s) 181, 392, 601, 866
EciI GGCGGA 1 cut(s) 619
Ecl136II GAGCTC 1 cut(s) 993
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 995
Eco47I GGWCC 1 cut(s) 1270
Eco53kI GAGCTC 1 cut(s) 993
Eco57I CTGAAG 4 cut(s) 201, 1002, 1278, 1335
Eco88I CYCGRG 1 cut(s) 441
EcoICRI GAGCTC 1 cut(s) 993
EcoRI GAATTC 1 cut(s) 1292
EcoRII CCWGG 2 cut(s) 214, 843
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 995
EgeI GGCGCC 1 cut(s) 894
EheI GGCGCC 1 cut(s) 894
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 6 cut(s) 62, 251, 440, 901, 1375, 1382
FatI CATG 6 cut(s) 58, 247, 436, 897, 1371, 1378
FauI CCCGC 1 cut(s) 495
FbaI TGATCA 1 cut(s) 664
Fnu4HI GCNGC 4 cut(s) 195, 234, 891, 1170
FokI GGATG 6 cut(s) 493, 542, 586, 613, 722, 1118
FriOI GRGCYC 1 cut(s) 995
Fsp4HI GCNGC 4 cut(s) 195, 234, 891, 1170
FspBI CTAG 2 cut(s) 498, 1146
GlaI GCGC 3 cut(s) 310, 894, 1242
GluI GCNGC 4 cut(s) 195, 234, 891, 1170
GsaI CCCAGC 3 cut(s) 368, 585, 1032
GsuI CTGGAG 1 cut(s) 564
HaeII RGCGCY 1 cut(s) 896
HaeIII GGCC 3 cut(s) 284, 961, 1329
HapII CCGG 4 cut(s) 281, 416, 603, 1248
HhaI GCGC 3 cut(s) 311, 895, 1243
Hin1I GRCGYC 1 cut(s) 893
Hin1II CATG 6 cut(s) 62, 251, 440, 901, 1375, 1382
Hin6I GCGC 3 cut(s) 309, 893, 1241
HinP1I GCGC 3 cut(s) 309, 893, 1241
HinfI GANTC 5 cut(s) 5, 241, 352, 946, 1054
HpaII CCGG 4 cut(s) 281, 416, 603, 1248
HphI GGTGA 2 cut(s) 430, 1194
Hpy166II GTNNAC 2 cut(s) 412, 1355
Hpy188III TCNNGA 5 cut(s) 940, 1058, 1160, 1268, 1379
Hpy8I GTNNAC 2 cut(s) 412, 1355
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 3 cut(s) 158, 754, 1016
HpyCH4III ACNGT 2 cut(s) 301, 1013
HpyCH4IV ACGT 3 cut(s) 745, 881, 1048
HpyCH4V TGCA 6 cut(s) 116, 188, 194, 236, 1196, 1355
HpyF10VI GCNNNNNNNGC 5 cut(s) 194, 200, 446, 455, 1193
HpyF3I CTNAG 8 cut(s) 348, 459, 798, 852, 866, 1040, 1158, 1266
HpySE526I ACGT 3 cut(s) 745, 881, 1048
Hsp92I GRCGYC 1 cut(s) 893
Hsp92II CATG 6 cut(s) 62, 251, 440, 901, 1375, 1382
HspAI GCGC 3 cut(s) 309, 893, 1241
KasI GGCGCC 1 cut(s) 892
Ksp22I TGATCA 1 cut(s) 664
LguI GCTCTTC 1 cut(s) 866
LmnI GCTCC 6 cut(s) 214, 256, 446, 469, 545, 998
Lsp1109I GCAGC 3 cut(s) 206, 220, 1156
LweI GCATC 5 cut(s) 515, 581, 706, 1043, 1291
MabI ACCWGGT 1 cut(s) 214
MaeI CTAG 2 cut(s) 498, 1146
MaeII ACGT 3 cut(s) 745, 881, 1048
MaeIII GTNAC 3 cut(s) 19, 151, 1200
MflI RGATCY 5 cut(s) 374, 385, 590, 651, 1114
MhlI GDGCHC 2 cut(s) 995, 1357
MlsI TGGCCA 1 cut(s) 1329
MluCI AATT 4 cut(s) 223, 342, 1069, 1292
MluNI TGGCCA 1 cut(s) 1329
Mly113I GGCGCC 1 cut(s) 893
MlyI GAGTC 2 cut(s) 14, 361
MmeI TCCRAC 4 cut(s) 518, 717, 974, 1372
Mox20I TGGCCA 1 cut(s) 1329
MscI TGGCCA 1 cut(s) 1329
MseI TTAA 7 cut(s) 171, 341, 555, 674, 885, 1131, 1399
MslI CAYNNNNRTG 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 1329
MspI CCGG 4 cut(s) 281, 416, 603, 1248
MspR9I CCNGG 3 cut(s) 216, 416, 845
Mva1269I GAATGC 1 cut(s) 106
MvaI CCWGG 2 cut(s) 216, 845
MwoI GCNNNNNNNGC 5 cut(s) 194, 200, 446, 455, 1193
NarI GGCGCC 1 cut(s) 893
NciI CCSGG 1 cut(s) 416
NlaIII CATG 6 cut(s) 62, 251, 440, 901, 1375, 1382
NlaIV GGNNCC 4 cut(s) 387, 465, 653, 894
NmuCI GTSAC 2 cut(s) 151, 1200
NspI RCATGY 2 cut(s) 62, 440
PaeR7I CTCGAG 1 cut(s) 441
PagI TCATGA 1 cut(s) 1378
PciI ACATGT 1 cut(s) 58
PciSI GCTCTTC 1 cut(s) 866
PctI GAATGC 1 cut(s) 106
PfeI GAWTC 3 cut(s) 241, 946, 1054
PflMI CCANNNNNTGG 2 cut(s) 647, 977
PkrI GCNGC 4 cut(s) 196, 235, 892, 1171
PleI GAGTC 2 cut(s) 13, 360
PluTI GGCGCC 1 cut(s) 896
PpsI GAGTC 2 cut(s) 13, 360
PscI ACATGT 1 cut(s) 58
PshBI ATTAAT 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 995
Psp6I CCWGG 2 cut(s) 214, 843
PspFI CCCAGC 3 cut(s) 364, 581, 1028
PspGI CCWGG 2 cut(s) 214, 843
PspN4I GGNNCC 4 cut(s) 387, 465, 653, 894
PspPI GGNCC 1 cut(s) 1270
PspXI VCTCGAGB 1 cut(s) 441
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 5 cut(s) 374, 385, 590, 651, 1114
RsaI GTAC 2 cut(s) 75, 413
RsaNI GTAC 2 cut(s) 74, 412
RseI CAYNNNNRTG 1 cut(s) 63
SacI GAGCTC 1 cut(s) 995
SapI GCTCTTC 1 cut(s) 866
SaqAI TTAA 7 cut(s) 171, 341, 555, 674, 885, 1131, 1399
SatI GCNGC 4 cut(s) 195, 234, 891, 1170
Sau96I GGNCC 1 cut(s) 1270
SchI GAGTC 2 cut(s) 14, 361
ScrFI CCNGG 3 cut(s) 216, 416, 845
SduI GDGCHC 2 cut(s) 995, 1357
SexAI ACCWGGT 1 cut(s) 214
SfaNI GCATC 5 cut(s) 515, 581, 706, 1043, 1291
SfoI GGCGCC 1 cut(s) 894
Sfr274I CTCGAG 1 cut(s) 441
SinI GGWCC 1 cut(s) 1270
SlaI CTCGAG 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 2 cut(s) 441, 938
SmoI CTYRAG 2 cut(s) 441, 938
Sse9I AATT 4 cut(s) 223, 342, 1069, 1292
SsiI CCGC 3 cut(s) 502, 630, 890
SspDI GGCGCC 1 cut(s) 892
SspMI CTAG 2 cut(s) 498, 1146
SstI GAGCTC 1 cut(s) 995
StyD4I CCNGG 3 cut(s) 214, 414, 843
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 2 cut(s) 301, 1013
TaiI ACGT 3 cut(s) 748, 884, 1051
TaqI TCGA 3 cut(s) 27, 48, 442
TasI AATT 4 cut(s) 223, 342, 1069, 1292
TauI GCSGC 1 cut(s) 893
TfiI GAWTC 3 cut(s) 241, 946, 1054
Tru1I TTAA 7 cut(s) 171, 341, 555, 674, 885, 1131, 1399
Tru9I TTAA 7 cut(s) 171, 341, 555, 674, 885, 1131, 1399
TseFI GTSAC 2 cut(s) 151, 1200
TseI GCWGC 3 cut(s) 194, 233, 1169
Tsp45I GTSAC 2 cut(s) 151, 1200
TspDTI ATGAA 6 cut(s) 177, 407, 509, 588, 1142, 1395
TspGWI ACGGA 3 cut(s) 715, 772, 997
Van91I CCANNNNNTGG 2 cut(s) 647, 977
VneI GTGCAC 1 cut(s) 1353
VpaK11BI GGWCC 1 cut(s) 1270
VspI ATTAAT 1 cut(s) 341
XapI RAATTY 2 cut(s) 223, 1292
XceI RCATGY 2 cut(s) 62, 440
XhoI CTCGAG 1 cut(s) 441
XspI CTAG 2 cut(s) 498, 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.