pycom12g11100

Glycogen recognition site of AMP-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005701
Physical Location & Seq
Reverse (-)
13179932 .. 13184107
4176 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1737 bp
ATGGCGGCCTCCGTCTCTCACCTCCCCGCCTTCCTCTCCCTCTTCTCTCACAAGCTCTTCTTCTCCCACCCGCAGCAGCAATCCACTCTCGGATTCACCGCCCTACAACGCTGTCCACCGCCGCAGCGCTGCACCCTTCGCGCTTCTTCCGTCAAGAAGAAAACCAGGAAGGTAAAGAGCAATGCGGAGCTCTGCAATGATCTCCGGCAGTTCCTCACTGCGGTCGGACTTTCGGAATGTCACGTTCCATCCTTGAAGGAGCTCTCGCAGCACGGAAGGAATGATCTTGCAAACATTGTCAGACGAAGAGGATATAAACTTATAAGAGAGCTTCTTGTGGACTCGAGCAATACAGATACCAATGGTAATGCCGATACCGGATTTGCTGGAATTCAGGATGTAATCGATGATCGTAAAGAGATAGTAACAGTTTCAGTCTTGGAAAGTAATTCTGGTAGTCCTTCAGAAATTTTCACCCCTTCATCCTTGGGCGAACTTGATGACCTATCCTTGTCAACCACTGGTCGTGTCAAAGAAACCATTTCTTCTAGTTCAAATGTTGGTTTGGATCTAAACTCCGATGGACGTCCTCGCATGCCTTTAGAATCTGTTGATGGTTTGTCATTGGATGAAAAGGTCTTAGATATATGTCAGGATGGGAAGGTTGACAATATGGCTAATGAAGATTGCTCTTCAACCATAGTTTCTGTTTTGGAAGACCATTCTAGTTCAGATCTTGATCCAAGTCACAACTCTGATCATCATAGTTATCCGCCTTTAGAATCTCCACCCAATTTATCCTTGAAGGAAAAAGTGGCAAACTTTATGCAGAATGGAGAATTGGATGCAGTTGAAGATAATGTCTATGGCATATTAACTGAGAATAATGATGAAGAAATCAAGGAAGAATGTAGATTTGGAAACACTGAGGAGGTTCAAATAAGAATTCCTAAAAATGTGCTTGACAGAAGTGATGCAATTATGGCATTGAATCGAAGGACATCAACATCAACTACAGCAGTTGATCCCTCTCTTAGGGAGGATAGTTCATCAACTGAGGGGCTGAGTTCTCTATATGACAAGGATTTAGATGATAAGACAAGTGAAAGTGAGGATCAACTTGAACTTAATCATCTCAAGTTCATGCTGCATCAGAAGGAGATGGAATTGTCACAGTTGAAAGAACAGATTCAGAAGGAAGAGCTTTTTTTGTCAAACTTGCAAACCAAATCTGAAATGGCAATGAGCAAAGCACAAAAGCTTGTCTCTGAAAAAGATGCAGAGTTAATTGCTGATGAAGAAAGCCTTTCAGGATTAGTGGAGGTTGAGATTCAGTACCAGGGAGATGGCAAGATTGTGGAGGTGACTGGTAGCTTCAATGGTTGGTATCATCAGATTGAAATGGATCCACAGCCCTCATCTAGTATCATAGAGAGGGAACCTAGACTATGGTCAACAATGCTGTGGCTTTATCCAGGGACGTATGAGGGAAGTCCACTATCTGCCTTTCACCGCGACAAGGAGCCAGATCATCTACTACAATTGAAGTTGAAGCAGTCTAAGCCTATGCCATCTGAACAAGAAAGTGAATTGAAGACCACGCACGCAGAAGGCGATACGGGGCGGGCAAAGTCGAGAAGCGAAATGGTCTTATGCCTCGCAATGTATATATATCCGCCAGTCGCCATGGCTTTTAAGTTCGTAATGATTTTTGTTTTCCTGGTAGGTTTGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMPK1_CBM

Protein Analysis

579

Amino Acids

64.41

Weight (kDa)

4.86

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 441 - 496 2.3e-06 Glycogen recognition site of AMP-activated protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 323
AatII GACGTC 1 cut(s) 589
AccII CGCG 2 cut(s) 141, 1515
AclWI GGATC 6 cut(s) 576, 734, 1019, 1122, 1400, 1413
AcsI RAATTY 3 cut(s) 390, 468, 945
AcuI CTGAAG 1 cut(s) 447
AcyI GRCGYC 1 cut(s) 586
AfaI GTAC 1 cut(s) 1337
AfeI AGCGCT 1 cut(s) 128
AfiI CCNNNNNNNGG 3 cut(s) 220, 1035, 1519
AjnI CCWGG 4 cut(s) 164, 1338, 1474, 1719
AjuI GAANNNNNNNTTGG 4 cut(s) 824, 856, 900, 932
AluBI AGCT 7 cut(s) 55, 190, 262, 331, 1204, 1261, 1374
AluI AGCT 7 cut(s) 55, 190, 262, 331, 1204, 1261, 1374
Alw21I GWGCWC 2 cut(s) 192, 264
Alw26I GTCTC 2 cut(s) 19, 1270
AlwI GGATC 6 cut(s) 576, 734, 1019, 1122, 1400, 1413
Ama87I CYCGRG 1 cut(s) 343
Aor51HI AGCGCT 1 cut(s) 128
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 6 cut(s) 73, 76, 124, 129, 268, 1147
ApoI RAATTY 3 cut(s) 390, 468, 945
Asp700I GAANNNNTTC 1 cut(s) 1188
AspLEI GCGC 2 cut(s) 129, 143
AsuHPI GGTGA 5 cut(s) 11, 88, 466, 1375, 1502
AvaI CYCGRG 1 cut(s) 343
BamHI GGATCC 1 cut(s) 1405
BanII GRGCYC 2 cut(s) 192, 264
BbsI GAAGAC 2 cut(s) 723, 1601
Bbv12I GWGCWC 2 cut(s) 192, 264
BbvI GCAGC 6 cut(s) 85, 88, 116, 136, 280, 1134
BccI CCATC 7 cut(s) 256, 575, 608, 650, 1156, 1340, 1579
BciT130I CCWGG 4 cut(s) 166, 1340, 1476, 1721
BclI TGATCA 1 cut(s) 757
BcoDI GTCTC 2 cut(s) 19, 1270
BfaI CTAG 4 cut(s) 549, 726, 1422, 1443
BfmI CTRYAG 1 cut(s) 1014
BfoI RGCGCY 1 cut(s) 130
BglII AGATCT 1 cut(s) 733
BisI GCNGC 8 cut(s) 6, 74, 77, 122, 125, 130, 269, 1148
BlsI GCNGC 8 cut(s) 7, 75, 78, 123, 126, 131, 270, 1149
Bme1390I CCNGG 4 cut(s) 166, 1340, 1476, 1721
BmeT110I CYCGRG 1 cut(s) 343
BmiI GGNNCC 3 cut(s) 1407, 1440, 1524
BmrFI CCNGG 4 cut(s) 166, 1340, 1476, 1721
BmsI GCATC 4 cut(s) 835, 964, 1159, 1267
BoxI GACNNNNGTC 1 cut(s) 1450
BpiI GAAGAC 2 cut(s) 723, 1601
BpuEI CTTGAG 1 cut(s) 1121
Bsa29I ATCGAT 1 cut(s) 405
BsaBI GATNNNNATC 1 cut(s) 738
BsaHI GRCGYC 1 cut(s) 586
BsaJI CCNNGG 4 cut(s) 486, 1339, 1475, 1686
BsaWI WCCGGW 1 cut(s) 377
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 1035, 1519
Bse1I ACTGG 3 cut(s) 526, 1372, 1679
Bse3DI GCAATG 4 cut(s) 187, 202, 1248, 1668
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 4 cut(s) 166, 1340, 1476, 1721
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 4 cut(s) 486, 1339, 1475, 1686
BseGI GGATG 6 cut(s) 248, 403, 482, 634, 661, 850
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 3 cut(s) 220, 1035, 1519
BseMI GCAATG 4 cut(s) 187, 202, 1248, 1668
BseMII CTCAG 4 cut(s) 870, 918, 1047, 1055
BseNI ACTGG 3 cut(s) 526, 1372, 1679
BseRI GAGGAG 1 cut(s) 944
BseXI GCAGC 6 cut(s) 85, 88, 116, 136, 280, 1134
BsgI GTGCAG 1 cut(s) 115
Bsh1236I CGCG 2 cut(s) 141, 1515
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 1 cut(s) 8
BshVI ATCGAT 1 cut(s) 405
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 192, 264
BsiHKCI CYCGRG 1 cut(s) 343
BsiSI CCGG 2 cut(s) 205, 378
BslFI GGGAC 1 cut(s) 1492
BslI CCNNNNNNNGG 3 cut(s) 220, 1035, 1519
BsmAI GTCTC 2 cut(s) 19, 1270
BsmBI CGTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 1492
BsnI GGCC 1 cut(s) 8
BsoBI CYCGRG 1 cut(s) 343
Bsp1286I GDGCHC 2 cut(s) 192, 264
Bsp19I CCATGG 1 cut(s) 1686
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 4 cut(s) 871, 919, 1048, 1056
BspDI ATCGAT 1 cut(s) 405
BspFNI CGCG 2 cut(s) 141, 1515
BspLI GGNNCC 3 cut(s) 1407, 1440, 1524
BspPI GGATC 6 cut(s) 576, 734, 1019, 1122, 1400, 1413
BspQI GCTCTTC 3 cut(s) 62, 697, 1194
BsrDI GCAATG 4 cut(s) 187, 202, 1248, 1668
BsrI ACTGG 3 cut(s) 526, 1372, 1679
BssECI CCNNGG 4 cut(s) 486, 1339, 1475, 1686
BssNI GRCGYC 1 cut(s) 586
BssT1I CCWWGG 2 cut(s) 486, 1686
Bst2UI CCWGG 4 cut(s) 166, 1340, 1476, 1721
Bst4CI ACNGT 2 cut(s) 430, 1176
Bst6I CTCTTC 5 cut(s) 47, 62, 301, 697, 1194
BstACI GRCGYC 1 cut(s) 586
BstC8I GCNNGC 3 cut(s) 596, 1605, 1626
BstDEI CTNAG 7 cut(s) 640, 879, 927, 1034, 1056, 1064, 1560
BstDSI CCRYGG 1 cut(s) 1686
BstENI CCTNNNNNAGG 1 cut(s) 1033
BstF5I GGATG 6 cut(s) 248, 403, 482, 634, 661, 850
BstFNI CGCG 2 cut(s) 141, 1515
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 2 cut(s) 129, 143
BstMAI GTCTC 2 cut(s) 19, 1270
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 4 cut(s) 138, 268, 983, 1561
BstNI CCWGG 4 cut(s) 166, 1340, 1476, 1721
BstNSI RCATGY 1 cut(s) 598
BstPAI GACNNNNGTC 1 cut(s) 1450
BstSCI CCNGG 4 cut(s) 164, 1338, 1474, 1719
BstSFI CTRYAG 1 cut(s) 1014
BstUI CGCG 2 cut(s) 141, 1515
BstV1I GCAGC 6 cut(s) 85, 88, 116, 136, 280, 1134
BstV2I GAAGAC 2 cut(s) 723, 1601
BstX2I RGATCY 3 cut(s) 568, 733, 1405
BstXI CCANNNNNNTGG 1 cut(s) 1346
BstYI RGATCY 3 cut(s) 568, 733, 1405
Bsu15I ATCGAT 1 cut(s) 405
BsuRI GGCC 1 cut(s) 8
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 1686
BtsCI GGATG 6 cut(s) 248, 403, 482, 634, 661, 850
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 3 cut(s) 216, 519, 924
Cac8I GCNNGC 3 cut(s) 596, 1605, 1626
CfoI GCGC 2 cut(s) 129, 143
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 1 cut(s) 1336
CviAII CATG 3 cut(s) 595, 1144, 1687
CviQI GTAC 1 cut(s) 1336
DdeI CTNAG 7 cut(s) 640, 879, 927, 1034, 1056, 1064, 1560
Eam1104I CTCTTC 5 cut(s) 47, 62, 301, 697, 1194
EarI CTCTTC 5 cut(s) 47, 62, 301, 697, 1194
EciI GGCGGA 2 cut(s) 762, 1665
Ecl136II GAGCTC 2 cut(s) 190, 262
Eco130I CCWWGG 2 cut(s) 486, 1686
Eco24I GRGCYC 2 cut(s) 192, 264
Eco47III AGCGCT 1 cut(s) 128
Eco53kI GAGCTC 2 cut(s) 190, 262
Eco57I CTGAAG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 343
EcoICRI GAGCTC 2 cut(s) 190, 262
EcoNI CCTNNNNNAGG 1 cut(s) 1033
EcoRI GAATTC 2 cut(s) 390, 945
EcoRII CCWGG 4 cut(s) 164, 1338, 1474, 1719
EcoT14I CCWWGG 2 cut(s) 486, 1686
EcoT38I GRGCYC 2 cut(s) 192, 264
ErhI CCWWGG 2 cut(s) 486, 1686
Esp3I CGTCTC 1 cut(s) 19
FaeI CATG 3 cut(s) 598, 1147, 1690
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 1290, 1322
FaqI GGGAC 1 cut(s) 1492
FatI CATG 3 cut(s) 594, 1143, 1686
FauI CCCGC 3 cut(s) 34, 78, 1617
FbaI TGATCA 1 cut(s) 757
Fnu4HI GCNGC 8 cut(s) 6, 74, 77, 122, 125, 130, 269, 1148
FokI GGATG 6 cut(s) 235, 410, 469, 641, 668, 857
FriOI GRGCYC 2 cut(s) 192, 264
Fsp4HI GCNGC 8 cut(s) 6, 74, 77, 122, 125, 130, 269, 1148
FspBI CTAG 4 cut(s) 549, 726, 1422, 1443
GlaI GCGC 2 cut(s) 128, 142
GluI GCNGC 8 cut(s) 6, 74, 77, 122, 125, 130, 269, 1148
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 1 cut(s) 8
HapII CCGG 2 cut(s) 205, 378
HhaI GCGC 2 cut(s) 129, 143
Hin1I GRCGYC 1 cut(s) 586
Hin1II CATG 3 cut(s) 598, 1147, 1690
Hin6I GCGC 2 cut(s) 127, 141
HinP1I GCGC 2 cut(s) 127, 141
HincII GTYRAC 3 cut(s) 516, 667, 1455
HindII GTYRAC 3 cut(s) 516, 667, 1455
HindIII AAGCTT 1 cut(s) 1259
HinfI GANTC 7 cut(s) 93, 341, 605, 782, 991, 1189, 1330
HpaII CCGG 2 cut(s) 205, 378
HphI GGTGA 5 cut(s) 11, 88, 466, 1375, 1502
Hpy166II GTNNAC 6 cut(s) 116, 340, 516, 667, 1455, 1496
Hpy188III TCNNGA 6 cut(s) 154, 395, 653, 737, 1311, 1635
Hpy8I GTNNAC 6 cut(s) 116, 340, 516, 667, 1455, 1496
HpyCH4III ACNGT 2 cut(s) 430, 1176
HpyCH4IV ACGT 3 cut(s) 243, 586, 1481
HpyCH4V TGCA 9 cut(s) 132, 195, 290, 829, 848, 977, 1150, 1222, 1280
HpyF10VI GCNNNNNNNGC 4 cut(s) 138, 268, 983, 1561
HpyF3I CTNAG 7 cut(s) 640, 879, 927, 1034, 1056, 1064, 1560
HpySE526I ACGT 3 cut(s) 243, 586, 1481
Hsp92I GRCGYC 1 cut(s) 586
Hsp92II CATG 3 cut(s) 598, 1147, 1690
HspAI GCGC 2 cut(s) 127, 141
Ksp22I TGATCA 1 cut(s) 757
LguI GCTCTTC 3 cut(s) 62, 697, 1194
LmnI GCTCC 3 cut(s) 187, 259, 1522
Lsp1109I GCAGC 6 cut(s) 85, 88, 116, 136, 280, 1134
LweI GCATC 4 cut(s) 835, 964, 1159, 1267
MaeI CTAG 4 cut(s) 549, 726, 1422, 1443
MaeII ACGT 3 cut(s) 243, 586, 1481
MaeIII GTNAC 5 cut(s) 239, 424, 746, 1170, 1363
MfeI CAATTG 1 cut(s) 1541
MflI RGATCY 3 cut(s) 568, 733, 1405
MhlI GDGCHC 2 cut(s) 192, 264
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 1 cut(s) 205
MroXI GAANNNNTTC 1 cut(s) 1188
MseI TTAA 5 cut(s) 875, 1128, 1286, 1695, 1735
MspI CCGG 2 cut(s) 205, 378
MspR9I CCNGG 4 cut(s) 166, 1340, 1476, 1721
MunI CAATTG 1 cut(s) 1541
MvaI CCWGG 4 cut(s) 166, 1340, 1476, 1721
MvnI CGCG 2 cut(s) 141, 1515
MwoI GCNNNNNNNGC 4 cut(s) 138, 268, 983, 1561
NcoI CCATGG 1 cut(s) 1686
NlaIII CATG 3 cut(s) 598, 1147, 1690
NlaIV GGNNCC 3 cut(s) 1407, 1440, 1524
NmuCI GTSAC 4 cut(s) 239, 746, 1170, 1363
NspI RCATGY 1 cut(s) 598
PaeI GCATGC 1 cut(s) 598
PaeR7I CTCGAG 1 cut(s) 343
PciSI GCTCTTC 3 cut(s) 62, 697, 1194
PcsI WCGNNNNNNNCGW 1 cut(s) 1611
PdmI GAANNNNTTC 1 cut(s) 1188
PfeI GAWTC 6 cut(s) 93, 605, 782, 991, 1189, 1330
PkrI GCNGC 8 cut(s) 7, 75, 78, 123, 126, 131, 270, 1149
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
PshAI GACNNNNGTC 1 cut(s) 1450
PsiI TTATAA 1 cut(s) 323
Psp124BI GAGCTC 2 cut(s) 192, 264
Psp6I CCWGG 4 cut(s) 164, 1338, 1474, 1719
PspGI CCWGG 4 cut(s) 164, 1338, 1474, 1719
PspN4I GGNNCC 3 cut(s) 1407, 1440, 1524
PspXI VCTCGAGB 1 cut(s) 343
PsuI RGATCY 3 cut(s) 568, 733, 1405
RsaI GTAC 1 cut(s) 1337
RsaNI GTAC 1 cut(s) 1336
SacI GAGCTC 2 cut(s) 192, 264
SapI GCTCTTC 3 cut(s) 62, 697, 1194
SaqAI TTAA 5 cut(s) 875, 1128, 1286, 1695, 1735
SatI GCNGC 8 cut(s) 6, 74, 77, 122, 125, 130, 269, 1148
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 4 cut(s) 166, 1340, 1476, 1721
SduI GDGCHC 2 cut(s) 192, 264
SfaNI GCATC 4 cut(s) 835, 964, 1159, 1267
SfcI CTRYAG 1 cut(s) 1014
Sfr274I CTCGAG 1 cut(s) 343
SlaI CTCGAG 1 cut(s) 343
SmlI CTYRAG 2 cut(s) 343, 1136
SmoI CTYRAG 2 cut(s) 343, 1136
SphI GCATGC 1 cut(s) 598
SspMI CTAG 4 cut(s) 549, 726, 1422, 1443
SstI GAGCTC 2 cut(s) 192, 264
StyD4I CCNGG 4 cut(s) 164, 1338, 1474, 1719
StyI CCWWGG 2 cut(s) 486, 1686
TaaI ACNGT 2 cut(s) 430, 1176
TaiI ACGT 3 cut(s) 246, 589, 1484
TaqI TCGA 4 cut(s) 344, 405, 994, 1634
TauI GCSGC 2 cut(s) 8, 124
TfiI GAWTC 6 cut(s) 93, 605, 782, 991, 1189, 1330
Tru1I TTAA 5 cut(s) 875, 1128, 1286, 1695, 1735
Tru9I TTAA 5 cut(s) 875, 1128, 1286, 1695, 1735
TscAI CASTG 3 cut(s) 223, 526, 931
TseFI GTSAC 4 cut(s) 239, 746, 1170, 1363
TseI GCWGC 6 cut(s) 73, 76, 124, 129, 268, 1147
Tsp45I GTSAC 4 cut(s) 239, 746, 1170, 1363
TspDTI ATGAA 7 cut(s) 471, 645, 696, 906, 1038, 1132, 1311
TspGWI ACGGA 2 cut(s) 139, 288
TspRI CASTG 3 cut(s) 223, 526, 931
XagI CCTNNNNNAGG 1 cut(s) 1033
XapI RAATTY 3 cut(s) 390, 468, 945
XceI RCATGY 1 cut(s) 598
XcmI CCANNNNNNNNNTGG 1 cut(s) 1234
XhoI CTCGAG 1 cut(s) 343
XmnI GAANNNNTTC 1 cut(s) 1188
XspI CTAG 4 cut(s) 549, 726, 1422, 1443
ZraI GACGTC 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.