pycom12g12100

Cell number regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009470
Physical Location & Seq
Reverse (-)
14605975 .. 14607757
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ATGGCGAGCAACAACAGGGAGAATAATGTCCAGGAGGAATCGAACCCGCTTCTGGGCAAGCAATTCGAGGAGACCCAGAATGATACTGAGAAGCCCGCCAAGGCCTCGCCGGAGACTCAGTCGCCGGAGAAATCTCCCACGGAGATGGGGCATGTGAATGGCGGTGGTAATGGGTGCGCGTGGACCGCCGATGGGCTGCCGTTGGGGCACGGGAGCGTGATGGGTGAGCCCATGGGCCGGGCCCAGTGGGACTCCAGCCTCCTTGCCTGTCTTGGCCGCAATGATGAATTCTGTAGCAGCGATCTTGAAGTTTGTCTTCTTGGAAGTGTGGCTCCTTGTGTGTTGTATGGAAGTAATGCTGAGAGACTTGGATCTACTCCTGGGACGTTTGCAAATCACTGTTTGCCTTACTCTGGTCTATATCTGATTGGAAACACCTTTTTCGGTTGGAACTTCCTTGCACCGTGGTTTTCATATCCTAGCCGTACTGCCATCCGCCGCAAGTTCAACCTAGAGGCAAGGAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

177

Amino Acids

19.12

Weight (kDa)

5.2

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 83 - 173 2.5e-11 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 179
AciI CCGC 7 cut(s) 47, 96, 162, 186, 277, 496, 499
AclWI GGATC 1 cut(s) 379
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 1 cut(s) 287
AfaI GTAC 1 cut(s) 487
AfiI CCNNNNNNNGG 5 cut(s) 52, 53, 192, 237, 413
AgsI TTSAA 2 cut(s) 308, 508
AjnI CCWGG 2 cut(s) 30, 379
Alw26I GTCTC 3 cut(s) 65, 107, 358
AlwI GGATC 1 cut(s) 379
AoxI GGCC 4 cut(s) 102, 235, 240, 274
ApaI GGGCCC 1 cut(s) 244
ApeKI GCWGC 2 cut(s) 196, 297
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 4 cut(s) 183, 235, 240, 241
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 236
AvaII GGWCC 1 cut(s) 183
BaeGI GKGCMC 2 cut(s) 210, 244
BanII GRGCYC 2 cut(s) 231, 244
BbsI GAAGAC 1 cut(s) 308
BbvI GCAGC 2 cut(s) 183, 309
BccI CCATC 4 cut(s) 139, 185, 214, 500
BceAI ACGGC 2 cut(s) 184, 468
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciT130I CCWGG 2 cut(s) 32, 381
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 3 cut(s) 65, 107, 358
BfaI CTAG 2 cut(s) 480, 512
BfmI CTRYAG 1 cut(s) 292
BglI GCCNNNNNGGC 1 cut(s) 205
BisI GCNGC 4 cut(s) 197, 277, 298, 499
BlsI GCNGC 4 cut(s) 198, 278, 299, 500
Bme1390I CCNGG 3 cut(s) 32, 239, 381
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 4 cut(s) 183, 235, 240, 241
BmiI GGNNCC 2 cut(s) 242, 333
BmrFI CCNGG 3 cut(s) 32, 239, 381
BmrI ACTGGG 1 cut(s) 238
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 1 cut(s) 308
BpmI CTGGAG 1 cut(s) 238
BpuMI CCSGG 1 cut(s) 239
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 5 cut(s) 99, 138, 231, 380, 464
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 53, 192, 237, 413
Bse1I ACTGG 1 cut(s) 244
Bse3DI GCAATG 1 cut(s) 286
BseBI CCWGG 2 cut(s) 32, 381
BseDI CCNNGG 5 cut(s) 99, 138, 231, 380, 464
BseGI GGATG 1 cut(s) 492
BseLI CCNNNNNNNGG 5 cut(s) 52, 53, 192, 237, 413
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 3 cut(s) 78, 131, 351
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 2 cut(s) 210, 244
BseXI GCAGC 2 cut(s) 183, 309
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 4 cut(s) 104, 237, 242, 276
BsiSI CCGG 3 cut(s) 110, 125, 238
BslFI GGGAC 2 cut(s) 263, 397
BslI CCNNNNNNNGG 5 cut(s) 52, 53, 192, 237, 413
BsmAI GTCTC 3 cut(s) 65, 107, 358
BsmFI GGGAC 2 cut(s) 263, 397
BsnI GGCC 4 cut(s) 104, 237, 242, 276
Bso31I GGTCTC 1 cut(s) 65
Bsp120I GGGCCC 1 cut(s) 240
Bsp1286I GDGCHC 3 cut(s) 210, 231, 244
Bsp143I GATC 2 cut(s) 301, 371
Bsp19I CCATGG 1 cut(s) 231
BspACI CCGC 7 cut(s) 47, 96, 162, 186, 277, 496, 499
BspANI GGCC 4 cut(s) 104, 237, 242, 276
BspCNI CTCAG 3 cut(s) 79, 130, 352
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 2 cut(s) 242, 333
BspPI GGATC 1 cut(s) 379
BspTNI GGTCTC 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 286
BsrI ACTGG 1 cut(s) 244
BssECI CCNNGG 5 cut(s) 99, 138, 231, 380, 464
BssMI GATC 2 cut(s) 301, 371
BssT1I CCWWGG 2 cut(s) 99, 231
Bst2UI CCWGG 2 cut(s) 32, 381
Bst4CI ACNGT 2 cut(s) 401, 465
BstC8I GCNNGC 3 cut(s) 7, 59, 96
BstDEI CTNAG 3 cut(s) 87, 117, 360
BstDSI CCRYGG 3 cut(s) 138, 231, 464
BstF5I GGATG 1 cut(s) 492
BstFNI CGCG 1 cut(s) 179
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 2 cut(s) 304, 374
BstMAI GTCTC 3 cut(s) 65, 107, 358
BstMBI GATC 2 cut(s) 301, 371
BstMWI GCNNNNNNNGC 2 cut(s) 185, 205
BstNI CCWGG 2 cut(s) 32, 381
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 3 cut(s) 30, 237, 379
BstSFI CTRYAG 1 cut(s) 292
BstSLI GKGCMC 2 cut(s) 210, 244
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 2 cut(s) 183, 309
BstV2I GAAGAC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 371
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 1 cut(s) 371
BsuRI GGCC 4 cut(s) 104, 237, 242, 276
BtgI CCRYGG 3 cut(s) 138, 231, 464
BtsCI GGATG 1 cut(s) 492
BtsIMutI CAGTG 2 cut(s) 251, 397
Cac8I GCNNGC 3 cut(s) 7, 59, 96
CfoI GCGC 1 cut(s) 179
Cfr13I GGNCC 4 cut(s) 183, 235, 240, 241
Csp6I GTAC 1 cut(s) 486
CviAII CATG 2 cut(s) 152, 232
CviQI GTAC 1 cut(s) 486
DdeI CTNAG 3 cut(s) 87, 117, 360
DpnI GATC 2 cut(s) 303, 373
DpnII GATC 2 cut(s) 301, 371
EaeI YGGCCR 1 cut(s) 274
EciI GGCGGA 1 cut(s) 485
Eco130I CCWWGG 2 cut(s) 99, 231
Eco147I AGGCCT 1 cut(s) 104
Eco24I GRGCYC 2 cut(s) 231, 244
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 30, 379
EcoT14I CCWWGG 2 cut(s) 99, 231
EcoT38I GRGCYC 2 cut(s) 231, 244
ErhI CCWWGG 2 cut(s) 99, 231
FaeI CATG 2 cut(s) 155, 235
FaiI YATR 5 cut(s) 153, 233, 348, 421, 475
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 2 cut(s) 263, 397
FatI CATG 2 cut(s) 151, 231
FauI CCCGC 2 cut(s) 54, 103
Fnu4HI GCNGC 4 cut(s) 197, 277, 298, 499
FokI GGATG 1 cut(s) 479
FriOI GRGCYC 2 cut(s) 231, 244
Fsp4HI GCNGC 4 cut(s) 197, 277, 298, 499
FspBI CTAG 2 cut(s) 480, 512
GlaI GCGC 1 cut(s) 178
GluI GCNGC 4 cut(s) 197, 277, 298, 499
GsuI CTGGAG 1 cut(s) 238
HaeIII GGCC 4 cut(s) 104, 237, 242, 276
HapII CCGG 3 cut(s) 110, 125, 238
HhaI GCGC 1 cut(s) 179
Hin1II CATG 2 cut(s) 155, 235
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HinfI GANTC 3 cut(s) 38, 115, 251
HpaII CCGG 3 cut(s) 110, 125, 238
HphI GGTGA 1 cut(s) 236
Hpy166II GTNNAC 1 cut(s) 183
Hpy188I TCNGA 1 cut(s) 426
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 1 cut(s) 183
HpyCH4III ACNGT 2 cut(s) 401, 465
HpyCH4IV ACGT 1 cut(s) 386
HpyCH4V TGCA 2 cut(s) 392, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 205
HpyF3I CTNAG 3 cut(s) 87, 117, 360
HpySE526I ACGT 1 cut(s) 386
Hsp92II CATG 2 cut(s) 155, 235
HspAI GCGC 1 cut(s) 177
Kzo9I GATC 2 cut(s) 301, 371
LmnI GCTCC 2 cut(s) 213, 337
Lsp1109I GCAGC 2 cut(s) 183, 309
MaeI CTAG 2 cut(s) 480, 512
MaeII ACGT 1 cut(s) 386
MalI GATC 2 cut(s) 303, 373
MboI GATC 2 cut(s) 301, 371
MboII GAAGA 1 cut(s) 308
MflI RGATCY 1 cut(s) 371
MhlI GDGCHC 3 cut(s) 210, 231, 244
MluCI AATT 2 cut(s) 62, 287
MlyI GAGTC 2 cut(s) 109, 245
MmeI TCCRAC 1 cut(s) 428
MnlI CCTC 5 cut(s) 28, 61, 115, 269, 508
MslI CAYNNNNRTG 2 cut(s) 143, 156
MspI CCGG 3 cut(s) 110, 125, 238
MspR9I CCNGG 3 cut(s) 32, 239, 381
MvaI CCWGG 2 cut(s) 32, 381
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 2 cut(s) 185, 205
NciI CCSGG 1 cut(s) 239
NcoI CCATGG 1 cut(s) 231
NdeII GATC 2 cut(s) 301, 371
NlaIII CATG 2 cut(s) 155, 235
NlaIV GGNNCC 2 cut(s) 242, 333
NspI RCATGY 1 cut(s) 155
PceI AGGCCT 1 cut(s) 104
PfeI GAWTC 1 cut(s) 38
PflFI GACNNNGTC 1 cut(s) 118
PfoI TCCNGGA 1 cut(s) 30
PkrI GCNGC 4 cut(s) 198, 278, 299, 500
PleI GAGTC 2 cut(s) 109, 245
PpsI GAGTC 2 cut(s) 109, 245
Psp6I CCWGG 2 cut(s) 30, 379
PspGI CCWGG 2 cut(s) 30, 379
PspN4I GGNNCC 2 cut(s) 242, 333
PspOMI GGGCCC 1 cut(s) 240
PspPI GGNCC 4 cut(s) 183, 235, 240, 241
PsuI RGATCY 1 cut(s) 371
PsyI GACNNNGTC 1 cut(s) 118
RsaI GTAC 1 cut(s) 487
RsaNI GTAC 1 cut(s) 486
RseI CAYNNNNRTG 2 cut(s) 143, 156
SatI GCNGC 4 cut(s) 197, 277, 298, 499
Sau3AI GATC 2 cut(s) 301, 371
Sau96I GGNCC 4 cut(s) 183, 235, 240, 241
SchI GAGTC 2 cut(s) 109, 245
ScrFI CCNGG 3 cut(s) 32, 239, 381
SduI GDGCHC 3 cut(s) 210, 231, 244
SetI ASST 3 cut(s) 389, 440, 513
SfcI CTRYAG 1 cut(s) 292
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 2 cut(s) 143, 156
Sse9I AATT 2 cut(s) 62, 287
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 7 cut(s) 47, 96, 162, 186, 277, 496, 499
SspMI CTAG 2 cut(s) 480, 512
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 3 cut(s) 30, 237, 379
StyI CCWWGG 2 cut(s) 99, 231
TaaI ACNGT 2 cut(s) 401, 465
TaiI ACGT 1 cut(s) 389
TaqI TCGA 2 cut(s) 41, 66
TasI AATT 2 cut(s) 62, 287
TauI GCSGC 2 cut(s) 279, 501
TfiI GAWTC 1 cut(s) 38
TscAI CASTG 2 cut(s) 251, 404
TseI GCWGC 2 cut(s) 196, 297
TspDTI ATGAA 2 cut(s) 300, 462
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 2 cut(s) 251, 404
Tth111I GACNNNGTC 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 183
XapI RAATTY 1 cut(s) 287
XceI RCATGY 1 cut(s) 155
XspI CTAG 2 cut(s) 480, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.