pycom12g19560

Ppx/GppA phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003859
Physical Location & Seq
Reverse (-)
21362872 .. 21363303
432 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g19560.1

Sequence Viewer

Length: 432 bp
ATGAGGGACCATATCAGGTTGGTGATTCAGGAATCTGGGTTGGTTGAAAAGATTCAATCTTGCGGCTTTGAAGTCGTTGCGGGGTCTTCCGGTACGATCAAGGCTGTTGAGAAGGCGGTCCCTTTCGGGTATAGCAATGTGAGCAATATGCTGCAGGTGGGCAATTTGGTTTCTTTTGGGGATGGCAAGAGGGCTTGGAAGCTGAGCAGAGGAGAGGTGAAGGGTGTTGTGGGGAGTTTGTGCGGCGGAGGAGAGCCGGAGAGGGTTAGGAAGGAGAAGTTTTTTAAAAGGCCGTCGGAGTTTATGGTTGCAGGGGCAGTGCTGTTGGAGGAGATATTCGAGGTGCTTGGAATTGAGGAGATGGAGGTTTCTGGTTATGCATTGGCAGAAGGGGTTGTTGCAGAGAGTTTGGCAAGGGGTAGTGAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HD Ppx-GppA

Protein Analysis

144

Amino Acids

15.3

Weight (kDa)

5.33

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ppx-GppA PF02541 68 - 138 3.6e-06 Ppx/GppA phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 145
Acc36I ACCTGC 1 cut(s) 145
AciI CCGC 5 cut(s) 63, 80, 116, 243, 246
AfaI GTAC 1 cut(s) 94
AgsI TTSAA 3 cut(s) 47, 56, 71
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
AoxI GGCC 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 151
Asp700I GAANNNNTTC 1 cut(s) 51
AspS9I GGNCC 2 cut(s) 7, 118
AsuHPI GGTGA 2 cut(s) 34, 229
AvaII GGWCC 2 cut(s) 7, 118
BbsI GAAGAC 1 cut(s) 78
BbvI GCAGC 1 cut(s) 138
BccI CCATC 2 cut(s) 176, 355
BceAI ACGGC 1 cut(s) 277
BfmI CTRYAG 1 cut(s) 152
BfuAI ACCTGC 1 cut(s) 145
BisI GCNGC 3 cut(s) 64, 152, 244
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 3 cut(s) 65, 153, 245
Bme18I GGWCC 2 cut(s) 7, 118
BmgT120I GGNCC 2 cut(s) 7, 118
BmiI GGNNCC 2 cut(s) 8, 120
BpiI GAAGAC 1 cut(s) 78
Bpu1102I GCTNAGC 1 cut(s) 203
BsaWI WCCGGW 1 cut(s) 89
Bse3DI GCAATG 1 cut(s) 142
BseGI GGATG 1 cut(s) 187
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 194
BseRI GAGGAG 4 cut(s) 225, 264, 344, 371
BseXI GCAGC 1 cut(s) 138
BshFI GGCC 1 cut(s) 292
BsiSI CCGG 2 cut(s) 90, 257
BslFI GGGAC 2 cut(s) 20, 104
BsmFI GGGAC 2 cut(s) 20, 104
BsnI GGCC 1 cut(s) 292
Bsp143I GATC 1 cut(s) 96
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 5 cut(s) 63, 80, 116, 243, 246
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 1 cut(s) 195
BspLI GGNNCC 2 cut(s) 8, 120
BspMAI CTGCAG 1 cut(s) 156
BspMI ACCTGC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 142
BssMI GATC 1 cut(s) 96
BstDEI CTNAG 1 cut(s) 203
BstF5I GGATG 1 cut(s) 187
BstKTI GATC 1 cut(s) 99
BstMBI GATC 1 cut(s) 96
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstSFI CTRYAG 1 cut(s) 152
BstV1I GCAGC 1 cut(s) 138
BstV2I GAAGAC 1 cut(s) 78
BsuRI GGCC 1 cut(s) 292
BtsCI GGATG 1 cut(s) 187
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 1 cut(s) 324
BveI ACCTGC 1 cut(s) 145
Cfr13I GGNCC 2 cut(s) 7, 118
Csp6I GTAC 1 cut(s) 93
CviJI RGCY 6 cut(s) 66, 104, 194, 202, 256, 292
CviKI_1 RGCY 6 cut(s) 66, 104, 194, 202, 256, 292
CviQI GTAC 1 cut(s) 93
DdeI CTNAG 1 cut(s) 203
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
DraI TTTAAA 1 cut(s) 286
EciI GGCGGA 1 cut(s) 261
Eco47I GGWCC 2 cut(s) 7, 118
EcoT22I ATGCAT 1 cut(s) 382
FaiI YATR 5 cut(s) 12, 132, 149, 305, 378
FaqI GGGAC 2 cut(s) 20, 104
FauI CCCGC 1 cut(s) 73
Fnu4HI GCNGC 3 cut(s) 64, 152, 244
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 3 cut(s) 64, 152, 244
GluI GCNGC 3 cut(s) 64, 152, 244
HaeIII GGCC 1 cut(s) 292
HapII CCGG 2 cut(s) 90, 257
HinfI GANTC 3 cut(s) 25, 32, 52
HpaII CCGG 2 cut(s) 90, 257
HphI GGTGA 2 cut(s) 34, 229
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 1 cut(s) 29
Hpy99I CGWCG 1 cut(s) 298
HpyAV CCTTC 4 cut(s) 106, 214, 265, 383
HpyCH4V TGCA 4 cut(s) 154, 311, 380, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 1 cut(s) 203
Kzo9I GATC 1 cut(s) 96
LpnPI CCDG 7 cut(s) 14, 21, 103, 140, 270, 297, 357
Lsp1109I GCAGC 1 cut(s) 138
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 1 cut(s) 78
MluCI AATT 2 cut(s) 163, 351
MmeI TCCRAC 2 cut(s) 276, 306
Mph1103I ATGCAT 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 51
MseI TTAA 2 cut(s) 285, 430
MspI CCGG 2 cut(s) 90, 257
MwoI GCNNNNNNNGC 1 cut(s) 141
NdeII GATC 1 cut(s) 96
NlaIV GGNNCC 2 cut(s) 8, 120
NsiI ATGCAT 1 cut(s) 382
PaqCI CACCTGC 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 3 cut(s) 25, 32, 52
PkrI GCNGC 3 cut(s) 65, 153, 245
PspN4I GGNNCC 2 cut(s) 8, 120
PspPI GGNCC 2 cut(s) 7, 118
PstI CTGCAG 1 cut(s) 156
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
SaqAI TTAA 2 cut(s) 285, 430
SatI GCNGC 3 cut(s) 64, 152, 244
Sau3AI GATC 1 cut(s) 96
Sau96I GGNCC 2 cut(s) 7, 118
SetI ASST 6 cut(s) 20, 159, 204, 219, 345, 369
SfcI CTRYAG 1 cut(s) 152
SinI GGWCC 2 cut(s) 7, 118
Sse9I AATT 2 cut(s) 163, 351
SsiI CCGC 5 cut(s) 63, 80, 116, 243, 246
TaqI TCGA 1 cut(s) 339
TasI AATT 2 cut(s) 163, 351
TauI GCSGC 2 cut(s) 66, 246
TfiI GAWTC 3 cut(s) 25, 32, 52
Tru1I TTAA 2 cut(s) 285, 430
Tru9I TTAA 2 cut(s) 285, 430
TscAI CASTG 1 cut(s) 324
TseI GCWGC 1 cut(s) 151
TspRI CASTG 1 cut(s) 324
VpaK11BI GGWCC 2 cut(s) 7, 118
XmnI GAANNNNTTC 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.