Rw3G006530

Ppx/GppA phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003859
Physical Location & Seq
Forward (+)
6148004 .. 6151505
3502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G006530.1

Sequence Viewer

Length: 1779 bp
ATGGCTTCTTCTTCTTCAACACTCTTTGCAGCCATTGACATGGGCACAAACTCATTCAAGATGATCATAATCCGAGCCTACCCAGATGGCAAGTTCCTCACCATTGATCGTCTCAGAGAGCCTGTGGTTCTTGGCCGTGACACATCATCATCCACAACCCCTTTTACACTCTCAGCTCAATCTCAGCTCATTGCTATTGAGGCCCTGAAGAAGTTTCAGAACACATTGATCTCCTACAAAATCAGCAAAGCTCAAATCCGCTGTGTTGCAACAGCAGCTGTGCGTGAGGCTGTGAATAAAGGTGAGTTCTTGAAATGTGTTAGTGAGAAGATAGGTTTTGAGGTTGATGTGTTGCCTGGTGAAGAAGAAGCTAGGCTTGTGTATCTGGGTATGCTTCAGTTTTGGCCTATATATGAGAAATTGGTTCTGGGTGTTGATATCGGAGGTGGGTCTACTGAGTTTGTGATTGGGAAGCAGGGGAAAGTTGTTTTTGGGGCTTCATTGAAGTTGGGGCATGTGAATTTGACCCAGAAATTTGGAAGCAATAAAGAAAAGGTTGCTCAGATTAGGGAGCATATTGGGTTGGTTGTTAAAGAATCTGGGTTGGTTAGGAAGATTAAAGATTGTGGCTTTGAAGTTGTTGTTGGGTCCTCTGGTACTATTAAGGCAGTTGAGAAGGCAGTGTTTGATGGGTATGTGAATAAGAGCAATGTGATTGAGGTTAGTAATGTGGCTTCTTTCGGGGATGGTAGGAAAGATTGGAGGTTGAGTAGAGGAGAAGTGAAGGGTGTTGTTGAGAGTTTGTGTGGTGGAGGAGAGGGAGAGAAGATTAGGAGAGAAAAGTTCTTCGAAAGGCGGTCTGAGTTTATTGTTGCTGGGGCTGTGTTGTTGGAGGAGATATTTGAGGTGCTTGGAATTGAGGAAATGGAGGTTTCTGGTTATGCATTGGCAGAAGGTGTAATTGCAGAAAGTTTGGCTAAGGTTAACGGTGGCTATGATCTGAATGCCAATGCAAGGTGGTCATCTATTGTTCGGCTTGCGATGAGGTATAATGGCAAGAAGAGGATGAAAGCTGCTTCTCAGTGTGCCATCATTGCAAAGGGGATTTTTGAAGGTTTAAGAAAATGTGATGACCTAGCTGATAATCATGCTGCCTCCTTAGATGACAAGGATCTTGAATATCTTGAAGCTGCAAGCTTGCTTCACAATATTGGGGTTTTCATTGGGAAAAAAGGTTACCACAAGCACTCTTATTGTATTATCATGAATGGCGAGCATCTCCATGGTTACAGTTCTGAGGAGGTTAAGCTGATAGCACTACTTGCAAGACATCATAGGAAGCAATTACCAAGCTTTGATCCTACTTGCTTTCAACAGTTTCCAAGAGAGGTAGCTTCTTACAGAACTATAAAGTTGTATTCCTGTGTTTTTTTTCCCCTTTCGATTATATATGTAGTTGAAAACAATATTTATTTGTTCTTTTATGTTTGTTTATGCATACTATCTAATTTTACTTACAGATCACAAAGAAATTCAGATATCTATGCACAATTATCCGTGTTTCTTTTGCACTACAACAAATGGAATTCTCAAACTATGAAGGTTTTAAGCTGGTATTTCTCTGCTCTGGTTTCCCACCCTTATAAAGTAGGGTTTCTTTGTCTCTGCAGTTGTAGAATCCTAATGATAAAACACCAGAGGTCAAACATATCTTGTACTATTAATGTTAGATTAGTATTATTGTTTTTGGTGAGTGAAATCACCCCCTTATTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HD Ppx-GppA

Protein Analysis

592

Amino Acids

66.14

Weight (kDa)

8.93

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ppx-GppA PF02541 28 - 326 1.7e-47 Ppx/GppA phosphatase family
Ppx-GppA_III PF21447 356 - 450 1.5e-14 Ppx/GppA phosphatase, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1644
AccI GTMKAC 1 cut(s) 452
AciI CCGC 2 cut(s) 259, 856
AclWI GGATC 2 cut(s) 1179, 1352
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 4 cut(s) 520, 533, 1531, 1585
AcuI CTGAAG 2 cut(s) 227, 380
AfaI GTAC 2 cut(s) 658, 1717
AfiI CCNNNNNNNGG 1 cut(s) 1014
AjnI CCWGG 1 cut(s) 355
AjuI GAANNNNNNNTTGG 2 cut(s) 627, 659
Alw26I GTCTC 2 cut(s) 116, 1667
AlwI GGATC 2 cut(s) 1179, 1352
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 3 cut(s) 133, 201, 404
ApeKI GCWGC 5 cut(s) 29, 275, 1073, 1151, 1190
ApoI RAATTY 4 cut(s) 520, 533, 1531, 1585
AseI ATTAAT 1 cut(s) 1722
AspS9I GGNCC 2 cut(s) 202, 648
AsuHPI GGTGA 5 cut(s) 91, 314, 371, 1753, 1762
AsuII TTCGAA 1 cut(s) 849
AvaII GGWCC 1 cut(s) 648
BaeGI GKGCMC 1 cut(s) 47
BarI GAAGNNNNNNTAC 2 cut(s) 718, 750
BbvI GCAGC 5 cut(s) 41, 287, 1060, 1138, 1177
BccI CCATC 4 cut(s) 80, 683, 740, 1097
BceAI ACGGC 1 cut(s) 120
BciT130I CCWGG 1 cut(s) 357
BclI TGATCA 1 cut(s) 63
BcoDI GTCTC 2 cut(s) 116, 1667
BfaI CTAG 2 cut(s) 372, 1136
BfmI CTRYAG 1 cut(s) 1666
BisI GCNGC 5 cut(s) 30, 276, 1074, 1152, 1191
BlsI GCNGC 5 cut(s) 31, 277, 1075, 1153, 1192
Bme1390I CCNGG 1 cut(s) 357
Bme18I GGWCC 1 cut(s) 648
BmgT120I GGNCC 2 cut(s) 202, 648
BmiI GGNNCC 1 cut(s) 649
BmrFI CCNGG 1 cut(s) 357
BmsI GCATC 1 cut(s) 1285
Bpu10I CCTNAGC 1 cut(s) 978
Bpu14I TTCGAA 1 cut(s) 849
BsaBI GATNNNNATC 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 1282
BsaXI ACNNNNNCTCC 2 cut(s) 754, 784
Bsc4I CCNNNNNNNGG 1 cut(s) 1014
Bse3DI GCAATG 3 cut(s) 189, 715, 1092
Bse8I GATNNNNATC 1 cut(s) 68
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 1282
BseGI GGATG 3 cut(s) 149, 751, 1071
BseJI GATNNNNATC 1 cut(s) 68
BseLI CCNNNNNNNGG 1 cut(s) 1014
BseMI GCAATG 3 cut(s) 189, 715, 1092
BseMII CTCAG 8 cut(s) 127, 186, 197, 447, 575, 852, 1094, 1287
BseRI GAGGAG 4 cut(s) 789, 828, 908, 1313
BseSI GKGCMC 1 cut(s) 47
BseXI GCAGC 5 cut(s) 41, 287, 1060, 1138, 1177
BseYI CCCAGC 1 cut(s) 875
BshFI GGCC 3 cut(s) 135, 203, 406
BslI CCNNNNNNNGG 1 cut(s) 1014
BsmAI GTCTC 2 cut(s) 116, 1667
BsmBI CGTCTC 1 cut(s) 116
BsmI GAATGC 1 cut(s) 1009
BsnI GGCC 3 cut(s) 135, 203, 406
Bsp119I TTCGAA 1 cut(s) 849
Bsp1286I GDGCHC 1 cut(s) 47
Bsp143I GATC 7 cut(s) 63, 106, 228, 997, 1171, 1357, 1520
Bsp19I CCATGG 1 cut(s) 1282
BspACI CCGC 2 cut(s) 259, 856
BspANI GGCC 3 cut(s) 135, 203, 406
BspCNI CTCAG 8 cut(s) 126, 185, 196, 448, 574, 853, 1093, 1288
BspHI TCATGA 1 cut(s) 1263
BspLI GGNNCC 1 cut(s) 649
BspMAI CTGCAG 1 cut(s) 1670
BspPI GGATC 2 cut(s) 1179, 1352
BspT104I TTCGAA 1 cut(s) 849
BsrDI GCAATG 3 cut(s) 189, 715, 1092
BssECI CCNNGG 1 cut(s) 1282
BssMI GATC 7 cut(s) 63, 106, 228, 997, 1171, 1357, 1520
BssT1I CCWWGG 1 cut(s) 1282
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 3 cut(s) 989, 1292, 1377
Bst6I CTCTTC 1 cut(s) 1055
BstAPI GCANNNNNTGC 1 cut(s) 1322
BstBI TTCGAA 1 cut(s) 849
BstC8I GCNNGC 4 cut(s) 1038, 1195, 1199, 1274
BstDSI CCRYGG 1 cut(s) 1282
BstEII GGTNACC 1 cut(s) 1235
BstF5I GGATG 3 cut(s) 149, 751, 1071
BstKTI GATC 7 cut(s) 66, 109, 231, 1000, 1174, 1360, 1523
BstMAI GTCTC 2 cut(s) 116, 1667
BstMBI GATC 7 cut(s) 63, 106, 228, 997, 1171, 1357, 1520
BstMWI GCNNNNNNNGC 4 cut(s) 200, 275, 1094, 1322
BstNI CCWGG 1 cut(s) 357
BstNSI RCATGY 1 cut(s) 518
BstPI GGTNACC 1 cut(s) 1235
BstSCI CCNGG 1 cut(s) 355
BstSFI CTRYAG 1 cut(s) 1666
BstSLI GKGCMC 1 cut(s) 47
BstV1I GCAGC 5 cut(s) 41, 287, 1060, 1138, 1177
BstX2I RGATCY 1 cut(s) 1171
BstXI CCANNNNNNTGG 2 cut(s) 40, 536
BstYI RGATCY 1 cut(s) 1171
BsuRI GGCC 3 cut(s) 135, 203, 406
BtgI CCRYGG 1 cut(s) 1282
BtgZI GCGATG 1 cut(s) 1055
BtsCI GGATG 3 cut(s) 149, 751, 1071
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 2 cut(s) 687, 1088
Cac8I GCNNGC 4 cut(s) 1038, 1195, 1199, 1274
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 1 cut(s) 1263
Cfr13I GGNCC 2 cut(s) 202, 648
Csp6I GTAC 2 cut(s) 657, 1716
CviAII CATG 5 cut(s) 40, 515, 1148, 1264, 1283
CviQI GTAC 2 cut(s) 657, 1716
DpnI GATC 7 cut(s) 65, 108, 230, 999, 1173, 1359, 1522
DpnII GATC 7 cut(s) 63, 106, 228, 997, 1171, 1357, 1520
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 1055
EarI CTCTTC 1 cut(s) 1055
Eco130I CCWWGG 1 cut(s) 1282
Eco32I GATATC 2 cut(s) 439, 1540
Eco47I GGWCC 1 cut(s) 648
Eco57I CTGAAG 2 cut(s) 227, 380
Eco91I GGTNACC 1 cut(s) 1235
EcoO109I RGGNCCY 2 cut(s) 202, 648
EcoO65I GGTNACC 1 cut(s) 1235
EcoRI GAATTC 1 cut(s) 1585
EcoRII CCWGG 1 cut(s) 355
EcoRV GATATC 2 cut(s) 439, 1540
EcoT14I CCWWGG 1 cut(s) 1282
EcoT22I ATGCAT 2 cut(s) 946, 1499
ErhI CCWWGG 1 cut(s) 1282
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 5 cut(s) 43, 518, 1151, 1267, 1286
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FatI CATG 5 cut(s) 39, 514, 1147, 1263, 1282
FbaI TGATCA 1 cut(s) 63
FblI GTMKAC 1 cut(s) 452
Fnu4HI GCNGC 5 cut(s) 30, 276, 1074, 1152, 1191
FokI GGATG 3 cut(s) 136, 758, 1078
Fsp4HI GCNGC 5 cut(s) 30, 276, 1074, 1152, 1191
FspBI CTAG 2 cut(s) 372, 1136
GluI GCNGC 5 cut(s) 30, 276, 1074, 1152, 1191
GsaI CCCAGC 1 cut(s) 879
HaeIII GGCC 3 cut(s) 135, 203, 406
Hin1II CATG 5 cut(s) 43, 518, 1151, 1267, 1286
HincII GTYRAC 1 cut(s) 985
HindII GTYRAC 1 cut(s) 985
HindIII AAGCTT 2 cut(s) 1195, 1351
HinfI GANTC 2 cut(s) 596, 1677
HpaI GTTAAC 1 cut(s) 985
HphI GGTGA 5 cut(s) 91, 314, 371, 1753, 1762
Hpy166II GTNNAC 2 cut(s) 453, 985
Hpy188I TCNGA 9 cut(s) 74, 116, 219, 443, 564, 862, 1002, 1297, 1537
Hpy188III TCNNGA 5 cut(s) 58, 310, 1175, 1184, 1264
Hpy8I GTNNAC 2 cut(s) 453, 985
HpyAV CCTTC 5 cut(s) 670, 778, 947, 1106, 1594
HpyCH4III ACNGT 3 cut(s) 989, 1292, 1377
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 275, 1094, 1322
Hsp92II CATG 5 cut(s) 43, 518, 1151, 1267, 1286
Ksp22I TGATCA 1 cut(s) 63
KspAI GTTAAC 1 cut(s) 985
Kzo9I GATC 7 cut(s) 63, 106, 228, 997, 1171, 1357, 1520
LmnI GCTCC 1 cut(s) 571
Lsp1109I GCAGC 5 cut(s) 41, 287, 1060, 1138, 1177
LweI GCATC 1 cut(s) 1285
MaeI CTAG 2 cut(s) 372, 1136
MaeIII GTNAC 3 cut(s) 137, 1235, 1286
MalI GATC 7 cut(s) 65, 108, 230, 999, 1173, 1359, 1522
MboI GATC 7 cut(s) 63, 106, 228, 997, 1171, 1357, 1520
MflI RGATCY 1 cut(s) 1171
MhlI GDGCHC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 870
Mph1103I ATGCAT 2 cut(s) 946, 1499
MseI TTAA 8 cut(s) 591, 618, 663, 984, 1118, 1305, 1607, 1722
MslI CAYNNNNRTG 2 cut(s) 38, 1281
MspA1I CMGCKG 2 cut(s) 261, 278
MspR9I CCNGG 1 cut(s) 357
Mva1269I GAATGC 1 cut(s) 1009
MvaI CCWGG 1 cut(s) 357
MwoI GCNNNNNNNGC 4 cut(s) 200, 275, 1094, 1322
NcoI CCATGG 1 cut(s) 1282
NdeII GATC 7 cut(s) 63, 106, 228, 997, 1171, 1357, 1520
NlaIII CATG 5 cut(s) 43, 518, 1151, 1267, 1286
NlaIV GGNNCC 1 cut(s) 649
NmuCI GTSAC 1 cut(s) 137
NsiI ATGCAT 2 cut(s) 946, 1499
NspI RCATGY 1 cut(s) 518
NspV TTCGAA 1 cut(s) 849
PagI TCATGA 1 cut(s) 1263
PctI GAATGC 1 cut(s) 1009
PfeI GAWTC 2 cut(s) 596, 1677
PkrI GCNGC 5 cut(s) 31, 277, 1075, 1153, 1192
PpuMI RGGWCCY 1 cut(s) 648
PshBI ATTAAT 1 cut(s) 1722
PsiI TTATAA 1 cut(s) 1644
Psp5II RGGWCCY 1 cut(s) 648
Psp6I CCWGG 1 cut(s) 355
PspEI GGTNACC 1 cut(s) 1235
PspFI CCCAGC 1 cut(s) 875
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 1 cut(s) 649
PspPI GGNCC 2 cut(s) 202, 648
PspPPI RGGWCCY 1 cut(s) 648
PstI CTGCAG 1 cut(s) 1670
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 1171
PvuII CAGCTG 1 cut(s) 278
RsaI GTAC 2 cut(s) 658, 1717
RsaNI GTAC 2 cut(s) 657, 1716
RseI CAYNNNNRTG 2 cut(s) 38, 1281
SaqAI TTAA 8 cut(s) 591, 618, 663, 984, 1118, 1305, 1607, 1722
SatI GCNGC 5 cut(s) 30, 276, 1074, 1152, 1191
Sau3AI GATC 7 cut(s) 63, 106, 228, 997, 1171, 1357, 1520
Sau96I GGNCC 2 cut(s) 202, 648
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 1 cut(s) 47
SfaNI GCATC 1 cut(s) 1285
SfcI CTRYAG 1 cut(s) 1666
SfuI TTCGAA 1 cut(s) 849
SinI GGWCC 1 cut(s) 648
SmiMI CAYNNNNRTG 2 cut(s) 38, 1281
SsiI CCGC 2 cut(s) 259, 856
SspI AATATT 2 cut(s) 1210, 1468
SspMI CTAG 2 cut(s) 372, 1136
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 1 cut(s) 1282
TaaI ACNGT 3 cut(s) 989, 1292, 1377
TaqI TCGA 2 cut(s) 849, 1442
TatI WGTACW 1 cut(s) 1715
TfiI GAWTC 2 cut(s) 596, 1677
Tru1I TTAA 8 cut(s) 591, 618, 663, 984, 1118, 1305, 1607, 1722
Tru9I TTAA 8 cut(s) 591, 618, 663, 984, 1118, 1305, 1607, 1722
TscAI CASTG 2 cut(s) 687, 1088
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 5 cut(s) 29, 275, 1073, 1151, 1190
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 5 cut(s) 489, 1082, 1210, 1280, 1613
TspGWI ACGGA 1 cut(s) 1546
TspRI CASTG 2 cut(s) 687, 1088
VpaK11BI GGWCC 1 cut(s) 648
VspI ATTAAT 1 cut(s) 1722
XapI RAATTY 4 cut(s) 520, 533, 1531, 1585
XceI RCATGY 1 cut(s) 518
XmiI GTMKAC 1 cut(s) 452
XspI CTAG 2 cut(s) 372, 1136
Zsp2I ATGCAT 2 cut(s) 946, 1499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.