RLG00000025278

Ppx/GppA phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003859
Physical Location & Seq
Reverse (-)
43551845 .. 43554834
2990 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025278

Sequence Viewer

Length: 1671 bp
ATGGCTTCTTCTTCTTCTTCAACACTATTTGCAGCCATTGACATGGGCACAAACTCATTCAAGATGATCATAATCCGAGCCTACCCAGATGGCAAGTTCCTCACCATTGATCATCTCAGAGAGCATGTGGTTCTTGGCCGTGACACATCATCATCGACCACAACCCCTTTTACACTCTCAGCTCAATCTCAGCTCGTTGCTATTGAGGCCCTGAAGAAGTTTCAGAACACATTGACCTCCTACAAAATCAGCAAAGCTCAAATCCGCTGTGTTGCCACTGCAGCTGTGCGTGAGGCTGTGAATAATGGTGAGTTCTTGAAATGTGTTAGTGAGAAGATAGGTTTGGAGGTTGATGTGTTGCCTGGTGAAGAAGAAGCTAGGCTTGTGTATCTGGGTATGCTTCAGTTTTGGCCTATATATGAGAAATTGGTTCTGGGTGTTGATATTGGAGGTGGGTCTACTGAGTTTGTGATTGGGAAGCAGGGAAAAGTTGTTTTTGGGGCTTCATTGAAGTTGGGGCATGTGAATTTGACCCAGAAATTTGGGAACAATAAAGAAAAGATTGCTCAGATTAGGGAGCATATTAGGTTGGTTGTTAAAGAATCTGGGTTGGTTAGGAAGATTAAAGATTGTGGCTTTGAAGTTGTTGTTGGGTCCTCTGGTACTATTAAGGCAGTTGAGAAGGCACTGTTTGATGGGTATGTGAATAAGAGCAATGTGATTGAGGTTAGCAATGTGGCTTCTTTTGGGGATGGTAGGAAAGATTGGAGGTTGAGTAGAGGAGAAGTGAAGGGTGTTGTTGAGAGTTTGTGTGGTGGAGGAGAGGGAGAGAAGATTAGGAGAGAAAAGTTCTTCGAAAGGCGGTCTGAGTTTATTGTTGCTGGGGCTGTGTTGTTGGAGGAGATATTTGAGGTGCTTGGAATTGAGGAAATGGAGGTTTCTGGTTATGCATTGGCAGAAGGTGTAATTGCAGAAAGCTTGGCTAAGGTTAACGGTGGCTATGATCTGAATGCCAATGCAAGGTGCTCATCTATTGTTCGGCTTGCGATGAGGTATAATGGCAAGAAGAGGATGAAAGCTGCTTCTCAGTGTGCCATCATTGCAAAGGAGATTTTTGAAGGTTTAAGAAAATGTGATGACCTAGTTGATAATCATGCTGCCTCCTTAGATGACAAGGATCTTGAATATCTTGAAGCTGCAAGCTTGCTTCACAATATTGGGGTTTTCATTGGGAAAAAAGGTTACCACAAGCACTCGTATTGTATTATCATGAATGGCGAGCATCTCCATGGTTACAGTTCTGAGGAGGTTAAGCTGATAGCACTACTTGCAAGACATCATAGGAAGCAATTACCAAGCTTTGATCCTACTTGCTTTCAACAGTTTCCAAGAGAGATCACAAAGAAATTCAGATATTTATGCACAATTATCCGTGTTTCTTTTGCACTACAACAAATGGAATTCTCAAACTATGAAGGTTTTAAGCTGGTAAGCTGTGTTGTAAAAGATCAAACCTCTTTACATGTTGTAAACCCTCTAGCTGAGGATATTGAGGATGAACTAAGAGAAGAATTGGAACAGTTCAAACTGGTATTCCTTAAAGAGCTGCTCATTGTTGCTCCTTCGAGTGTTCCAGATATGATGGATATTCGCATAAAAGATGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HD Ppx-GppA

Protein Analysis

557

Amino Acids

61.77

Weight (kDa)

6.91

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ppx-GppA PF02541 30 - 328 1.3e-48 Ppx/GppA phosphatase family
Ppx-GppA_III PF21447 343 - 488 1.8e-17 Ppx/GppA phosphatase, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 458
AciI CCGC 2 cut(s) 265, 862
AclWI GGATC 2 cut(s) 1185, 1358
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 4 cut(s) 526, 539, 1406, 1460
AcuI CTGAAG 2 cut(s) 233, 386
AfaI GTAC 1 cut(s) 664
AfiI CCNNNNNNNGG 1 cut(s) 1020
AflIII ACRYGT 1 cut(s) 1522
AjnI CCWGG 1 cut(s) 361
AjuI GAANNNNNNNTTGG 4 cut(s) 326, 358, 633, 665
Alw21I GWGCWC 1 cut(s) 1028
AlwI GGATC 2 cut(s) 1185, 1358
AoxI GGCC 3 cut(s) 136, 207, 410
ApeKI GCWGC 6 cut(s) 32, 281, 1079, 1157, 1196, 1606
ApoI RAATTY 4 cut(s) 526, 539, 1406, 1460
AspS9I GGNCC 2 cut(s) 208, 654
AsuHPI GGTGA 3 cut(s) 94, 320, 377
AsuII TTCGAA 1 cut(s) 855
AvaII GGWCC 1 cut(s) 654
BaeGI GKGCMC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 1028
BbvCI CCTCAGC 1 cut(s) 1542
BbvI GCAGC 6 cut(s) 44, 293, 1066, 1144, 1183, 1593
BccI CCATC 5 cut(s) 83, 689, 746, 1103, 1636
BceAI ACGGC 1 cut(s) 123
BciT130I CCWGG 1 cut(s) 363
BclI TGATCA 2 cut(s) 66, 109
BfaI CTAG 3 cut(s) 378, 1142, 1538
BfmI CTRYAG 1 cut(s) 279
BisI GCNGC 6 cut(s) 33, 282, 1080, 1158, 1197, 1607
BlsI GCNGC 6 cut(s) 34, 283, 1081, 1159, 1198, 1608
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 1 cut(s) 654
BmgT120I GGNCC 2 cut(s) 208, 654
BmiI GGNNCC 1 cut(s) 655
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 1291
Bpu10I CCTNAGC 2 cut(s) 984, 1542
Bpu14I TTCGAA 1 cut(s) 855
BsaBI GATNNNNATC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 1288
BsaXI ACNNNNNCTCC 2 cut(s) 760, 790
Bsc4I CCNNNNNNNGG 1 cut(s) 1020
Bse1I ACTGG 1 cut(s) 1593
Bse3DI GCAATG 3 cut(s) 721, 739, 1098
Bse8I GATNNNNATC 1 cut(s) 71
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 1288
BseGI GGATG 3 cut(s) 757, 1077, 1561
BseJI GATNNNNATC 1 cut(s) 71
BseLI CCNNNNNNNGG 1 cut(s) 1020
BseMI GCAATG 3 cut(s) 721, 739, 1098
BseMII CTCAG 9 cut(s) 130, 192, 203, 453, 581, 858, 1100, 1293, 1533
BseNI ACTGG 1 cut(s) 1593
BseRI GAGGAG 4 cut(s) 795, 834, 914, 1319
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 6 cut(s) 44, 293, 1066, 1144, 1183, 1593
BseYI CCCAGC 1 cut(s) 881
BshFI GGCC 3 cut(s) 138, 209, 412
BsiHKAI GWGCWC 1 cut(s) 1028
BslI CCNNNNNNNGG 1 cut(s) 1020
BsmI GAATGC 1 cut(s) 1015
BsnI GGCC 3 cut(s) 138, 209, 412
Bsp119I TTCGAA 1 cut(s) 855
Bsp1286I GDGCHC 2 cut(s) 50, 1028
Bsp143I GATC 7 cut(s) 66, 109, 1003, 1177, 1363, 1395, 1507
Bsp19I CCATGG 1 cut(s) 1288
BspACI CCGC 2 cut(s) 265, 862
BspANI GGCC 3 cut(s) 138, 209, 412
BspCNI CTCAG 9 cut(s) 129, 191, 202, 454, 580, 859, 1099, 1294, 1534
BspHI TCATGA 1 cut(s) 1269
BspLI GGNNCC 1 cut(s) 655
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 2 cut(s) 1185, 1358
BspT104I TTCGAA 1 cut(s) 855
BsrDI GCAATG 3 cut(s) 721, 739, 1098
BsrI ACTGG 1 cut(s) 1593
BssECI CCNNGG 1 cut(s) 1288
BssMI GATC 7 cut(s) 66, 109, 1003, 1177, 1363, 1395, 1507
BssT1I CCWWGG 1 cut(s) 1288
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 5 cut(s) 690, 995, 1298, 1383, 1581
Bst6I CTCTTC 1 cut(s) 1061
BstAPI GCANNNNNTGC 1 cut(s) 1328
BstBI TTCGAA 1 cut(s) 855
BstC8I GCNNGC 4 cut(s) 1044, 1201, 1205, 1280
BstDSI CCRYGG 1 cut(s) 1288
BstEII GGTNACC 1 cut(s) 1241
BstF5I GGATG 3 cut(s) 757, 1077, 1561
BstKTI GATC 7 cut(s) 69, 112, 1006, 1180, 1366, 1398, 1510
BstMBI GATC 7 cut(s) 66, 109, 1003, 1177, 1363, 1395, 1507
BstMWI GCNNNNNNNGC 4 cut(s) 206, 281, 1100, 1328
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 3 cut(s) 128, 524, 1526
BstPI GGTNACC 1 cut(s) 1241
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 50
BstV1I GCAGC 6 cut(s) 44, 293, 1066, 1144, 1183, 1593
BstX2I RGATCY 1 cut(s) 1177
BstXI CCANNNNNNTGG 2 cut(s) 43, 542
BstYI RGATCY 1 cut(s) 1177
BsuRI GGCC 3 cut(s) 138, 209, 412
BtgI CCRYGG 1 cut(s) 1288
BtgZI GCGATG 1 cut(s) 1061
BtsCI GGATG 3 cut(s) 757, 1077, 1561
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 3 cut(s) 276, 686, 1094
Cac8I GCNNGC 4 cut(s) 1044, 1201, 1205, 1280
CciI TCATGA 1 cut(s) 1269
Cfr13I GGNCC 2 cut(s) 208, 654
Csp6I GTAC 1 cut(s) 663
CviAII CATG 7 cut(s) 43, 125, 521, 1154, 1270, 1289, 1523
CviQI GTAC 1 cut(s) 663
DpnI GATC 7 cut(s) 68, 111, 1005, 1179, 1365, 1397, 1509
DpnII GATC 7 cut(s) 66, 109, 1003, 1177, 1363, 1395, 1507
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 1061
EarI CTCTTC 1 cut(s) 1061
Eco130I CCWWGG 1 cut(s) 1288
Eco47I GGWCC 1 cut(s) 654
Eco57I CTGAAG 2 cut(s) 233, 386
Eco91I GGTNACC 1 cut(s) 1241
EcoO109I RGGNCCY 2 cut(s) 208, 654
EcoO65I GGTNACC 1 cut(s) 1241
EcoRI GAATTC 1 cut(s) 1460
EcoRII CCWGG 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 1288
EcoT22I ATGCAT 1 cut(s) 952
ErhI CCWWGG 1 cut(s) 1288
FaeI CATG 7 cut(s) 46, 128, 524, 1157, 1273, 1292, 1526
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FatI CATG 7 cut(s) 42, 124, 520, 1153, 1269, 1288, 1522
FbaI TGATCA 2 cut(s) 66, 109
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 6 cut(s) 33, 282, 1080, 1158, 1197, 1607
FokI GGATG 3 cut(s) 764, 1084, 1568
Fsp4HI GCNGC 6 cut(s) 33, 282, 1080, 1158, 1197, 1607
FspBI CTAG 3 cut(s) 378, 1142, 1538
GluI GCNGC 6 cut(s) 33, 282, 1080, 1158, 1197, 1607
GsaI CCCAGC 1 cut(s) 885
HaeIII GGCC 3 cut(s) 138, 209, 412
Hin1II CATG 7 cut(s) 46, 128, 524, 1157, 1273, 1292, 1526
HincII GTYRAC 1 cut(s) 991
HindII GTYRAC 1 cut(s) 991
HindIII AAGCTT 3 cut(s) 976, 1201, 1357
HinfI GANTC 1 cut(s) 602
HpaI GTTAAC 1 cut(s) 991
HphI GGTGA 3 cut(s) 94, 320, 377
Hpy166II GTNNAC 3 cut(s) 459, 991, 1531
Hpy188I TCNGA 8 cut(s) 77, 119, 225, 570, 868, 1008, 1303, 1412
Hpy188III TCNNGA 6 cut(s) 61, 316, 1181, 1190, 1270, 1634
Hpy8I GTNNAC 3 cut(s) 459, 991, 1531
HpyAV CCTTC 6 cut(s) 676, 784, 953, 1112, 1469, 1632
HpyCH4III ACNGT 5 cut(s) 690, 995, 1298, 1383, 1581
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 281, 1100, 1328
Hsp92II CATG 7 cut(s) 46, 128, 524, 1157, 1273, 1292, 1526
Ksp22I TGATCA 2 cut(s) 66, 109
KspAI GTTAAC 1 cut(s) 991
Kzo9I GATC 7 cut(s) 66, 109, 1003, 1177, 1363, 1395, 1507
LmnI GCTCC 2 cut(s) 577, 1624
Lsp1109I GCAGC 6 cut(s) 44, 293, 1066, 1144, 1183, 1593
LweI GCATC 1 cut(s) 1291
MaeI CTAG 3 cut(s) 378, 1142, 1538
MaeIII GTNAC 3 cut(s) 140, 1241, 1292
MalI GATC 7 cut(s) 68, 111, 1005, 1179, 1365, 1397, 1509
MboI GATC 7 cut(s) 66, 109, 1003, 1177, 1363, 1395, 1507
MflI RGATCY 1 cut(s) 1177
MhlI GDGCHC 2 cut(s) 50, 1028
MmeI TCCRAC 1 cut(s) 876
Mph1103I ATGCAT 1 cut(s) 952
MseI TTAA 8 cut(s) 597, 624, 669, 990, 1124, 1311, 1482, 1599
MslI CAYNNNNRTG 2 cut(s) 41, 1287
MspA1I CMGCKG 2 cut(s) 267, 284
MspR9I CCNGG 1 cut(s) 363
Mva1269I GAATGC 1 cut(s) 1015
MvaI CCWGG 1 cut(s) 363
MwoI GCNNNNNNNGC 4 cut(s) 206, 281, 1100, 1328
NcoI CCATGG 1 cut(s) 1288
NdeII GATC 7 cut(s) 66, 109, 1003, 1177, 1363, 1395, 1507
NlaIII CATG 7 cut(s) 46, 128, 524, 1157, 1273, 1292, 1526
NlaIV GGNNCC 1 cut(s) 655
NmuCI GTSAC 1 cut(s) 140
NsiI ATGCAT 1 cut(s) 952
NspI RCATGY 3 cut(s) 128, 524, 1526
NspV TTCGAA 1 cut(s) 855
PagI TCATGA 1 cut(s) 1269
PciI ACATGT 1 cut(s) 1522
PctI GAATGC 1 cut(s) 1015
PfeI GAWTC 1 cut(s) 602
PkrI GCNGC 6 cut(s) 34, 283, 1081, 1159, 1198, 1608
PpuMI RGGWCCY 1 cut(s) 654
PscI ACATGT 1 cut(s) 1522
Psp5II RGGWCCY 1 cut(s) 654
Psp6I CCWGG 1 cut(s) 361
PspEI GGTNACC 1 cut(s) 1241
PspFI CCCAGC 1 cut(s) 881
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 655
PspPI GGNCC 2 cut(s) 208, 654
PspPPI RGGWCCY 1 cut(s) 654
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 1177
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 1 cut(s) 664
RsaNI GTAC 1 cut(s) 663
RseI CAYNNNNRTG 2 cut(s) 41, 1287
SaqAI TTAA 8 cut(s) 597, 624, 669, 990, 1124, 1311, 1482, 1599
SatI GCNGC 6 cut(s) 33, 282, 1080, 1158, 1197, 1607
Sau3AI GATC 7 cut(s) 66, 109, 1003, 1177, 1363, 1395, 1507
Sau96I GGNCC 2 cut(s) 208, 654
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 2 cut(s) 50, 1028
SfaNI GCATC 1 cut(s) 1291
SfcI CTRYAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 855
SinI GGWCC 1 cut(s) 654
SmiMI CAYNNNNRTG 2 cut(s) 41, 1287
SsiI CCGC 2 cut(s) 265, 862
SspI AATATT 1 cut(s) 1216
SspMI CTAG 3 cut(s) 378, 1142, 1538
StyD4I CCNGG 1 cut(s) 361
StyI CCWWGG 1 cut(s) 1288
TaaI ACNGT 5 cut(s) 690, 995, 1298, 1383, 1581
TaqI TCGA 3 cut(s) 155, 855, 1625
TfiI GAWTC 1 cut(s) 602
Tru1I TTAA 8 cut(s) 597, 624, 669, 990, 1124, 1311, 1482, 1599
Tru9I TTAA 8 cut(s) 597, 624, 669, 990, 1124, 1311, 1482, 1599
TscAI CASTG 3 cut(s) 283, 693, 1094
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 6 cut(s) 32, 281, 1079, 1157, 1196, 1606
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 6 cut(s) 495, 1088, 1216, 1286, 1488, 1572
TspGWI ACGGA 1 cut(s) 1421
TspRI CASTG 3 cut(s) 283, 693, 1094
VpaK11BI GGWCC 1 cut(s) 654
XapI RAATTY 4 cut(s) 526, 539, 1406, 1460
XceI RCATGY 3 cut(s) 128, 524, 1526
XmiI GTMKAC 1 cut(s) 458
XspI CTAG 3 cut(s) 378, 1142, 1538
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.