pycom12g19680

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005594
Physical Location & Seq
Forward (+)
21425953 .. 21428031
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1284 bp
ATGAGCCAGATTGAAGTATCGACGATGAAATCTCCTCTCACTCCACTTGCGACTCTGATTGGTCGTGAGCTTAGGGGTGAGAAAGTTGAGAAACCTTATTTGAAGTATGGGCAGGCTGGTTTGGCGAAGAAAGGAGAAGATTACTTTCTTATAAAAACTGATTGCCAAAGAATTCATTCGGATCCGTCAACATCATTTTCTGTCTTTGCGATTTTTGATGGGCATAATGGTATATCAGCTGCTATTTTTACCAAGGAGAATTTATTGGCTAATGTTTTGAGTGCCATTCCTCAAGGAACTAGTAGGGATGAATGGCTGCAAGCCCTTCCTCGGGCGCTGGTTGCAGGATTTGTCAAAACTGATATAGAATTTCAACAGAAAGGGGAAACTTCTGGTACAACAGTGACTTTTGTTGTGATTGATGATTGGACTGTGACTGTTGCATCTGTTGGGGATTCTCGATGTATATTAGACACACAGGGAGGTGTTGTTTCACTCTTAACTGTTGATCACAGGCTGGAAGAAAATGAAGAAGAGAGGGAGCGTGTTACTGCCAGTGGCGGTGAAGTAGGAAGGCTCAATATTTTTGGGGGCAATGAGGTTGGTCCCCTTCGCTGCTGGCCTGGTGGATTATGCCTTTCTAGGTCAATTGGTGACACAGATGTGGGAGAATACATTGTCCCCATACCCCATGTCAAGCAAGTGAAGCTTTCAAATGCTGGGGGAAGACTTATAATAGCTTCTGATGGCATCTGGGATGCTTTGTCTTCTGATATGGCTGCCAAGTCATGTCGAGGATTGCCTGCAGAGCTTGCTGCAAAGCTGGTCGTTAAGGAGGCTCTGAGGTCAAGGGGACTGAAAGATGATACAACCTGCGTAGTTGTTGACATAATCCCTTCTGGCCATCCTGTCTTACCTCCACCACCAATTAAAAAACCAAACATGCTTGCTTCACTTATCTTTGGGAAGAAAAGTCAGAACTCCGTGAACAAGACAAATAAACTTTCTGCTATCGGAGTTGTGGAGGAGTTGTTTGAAGAAGGTTCAGCAATGCTCGATGAAAGGTTGGGTAAGGATTCTCCTCTGGATGCAAATTCGGGGATTTTCCGTTGCGCAGTCTGCCAAGTGGATCAGCCCCAAGTTGAAGGTTTATCAGTAAACTCTGCGCCTTTCTTCTCCCCGGCATCAAAGCCATGGGAAGGCCCCTTCCTCTGCGCAAAGTGTCAGAACAAGAAAGACGCCATGGAAGGAAAAAGACTGACCAGACCCACAGTGGTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

428

Amino Acids

45.99

Weight (kDa)

5.89

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 61 - 280 1.3e-33 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 152, 734
Acc16I TGCGCA 2 cut(s) 1114, 1216
Acc36I ACCTGC 1 cut(s) 881
AciI CCGC 1 cut(s) 561
AclWI GGATC 3 cut(s) 176, 189, 1137
AcoI YGGCCR 1 cut(s) 901
AcsI RAATTY 4 cut(s) 171, 259, 368, 1093
AcyI GRCGYC 1 cut(s) 1239
AfaI GTAC 1 cut(s) 397
AfiI CCNNNNNNNGG 3 cut(s) 330, 331, 1199
AgsI TTSAA 6 cut(s) 14, 103, 374, 714, 1037, 1145
AhlI ACTAGT 1 cut(s) 299
AjnI CCWGG 1 cut(s) 622
AleI CACNNNNGTG 1 cut(s) 662
AluBI AGCT 6 cut(s) 70, 239, 709, 740, 811, 823
AluI AGCT 6 cut(s) 70, 239, 709, 740, 811, 823
AlwI GGATC 3 cut(s) 176, 189, 1137
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 3 cut(s) 620, 901, 1201
ApeKI GCWGC 5 cut(s) 239, 316, 615, 779, 815
ApoI RAATTY 4 cut(s) 171, 259, 368, 1093
Asp700I GAANNNNTTC 1 cut(s) 175
AspLEI GCGC 4 cut(s) 337, 1115, 1168, 1217
AspS9I GGNCC 2 cut(s) 605, 1202
AsuC2I CCSGG 1 cut(s) 1181
AsuHPI GGTGA 3 cut(s) 89, 575, 665
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 1 cut(s) 605
BaeI ACNNNNGTAYC 2 cut(s) 387, 420
BalI TGGCCA 1 cut(s) 903
BamHI GGATCC 1 cut(s) 181
BbsI GAAGAC 2 cut(s) 733, 759
BbvI GCAGC 5 cut(s) 226, 303, 602, 766, 802
BccI CCATC 3 cut(s) 212, 740, 912
BciT130I CCWGG 1 cut(s) 624
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 1181
BcuI ACTAGT 1 cut(s) 299
BfaI CTAG 2 cut(s) 300, 642
BfmI CTRYAG 1 cut(s) 804
BfoI RGCGCY 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 881
BisI GCNGC 5 cut(s) 240, 317, 616, 780, 816
BlsI GCNGC 5 cut(s) 241, 318, 617, 781, 817
Bme1390I CCNGG 2 cut(s) 624, 1181
Bme18I GGWCC 1 cut(s) 605
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 2 cut(s) 605, 1202
BmiI GGNNCC 3 cut(s) 183, 607, 1204
BmrFI CCNGG 2 cut(s) 624, 1181
BmsI GCATC 5 cut(s) 452, 748, 759, 1078, 1193
BpiI GAAGAC 2 cut(s) 733, 759
Bpu10I CCTNAGC 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 1181
BsaHI GRCGYC 1 cut(s) 1239
BsaJI CCNNGG 5 cut(s) 252, 329, 1179, 1193, 1242
BsaXI ACNNNNNCTCC 4 cut(s) 474, 504, 1016, 1046
Bsc4I CCNNNNNNNGG 3 cut(s) 330, 331, 1199
Bse1I ACTGG 1 cut(s) 555
Bse3DI GCAATG 2 cut(s) 601, 1056
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 5 cut(s) 252, 329, 1179, 1193, 1242
BseGI GGATG 4 cut(s) 313, 763, 904, 1093
BseLI CCNNNNNNNGG 3 cut(s) 330, 331, 1199
BseMI GCAATG 2 cut(s) 601, 1056
BseMII CTCAG 1 cut(s) 833
BseNI ACTGG 1 cut(s) 555
BseRI GAGGAG 3 cut(s) 24, 1040, 1071
BseXI GCAGC 5 cut(s) 226, 303, 602, 766, 802
BseYI CCCAGC 1 cut(s) 719
BshFI GGCC 3 cut(s) 622, 903, 1203
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 1 cut(s) 1181
BslFI GGGAC 3 cut(s) 591, 665, 867
BslI CCNNNNNNNGG 3 cut(s) 330, 331, 1199
BsmFI GGGAC 3 cut(s) 591, 665, 867
BsnI GGCC 3 cut(s) 622, 903, 1203
BsoBI CYCGRG 1 cut(s) 330
Bsp143I GATC 3 cut(s) 181, 508, 1129
Bsp19I CCATGG 2 cut(s) 1193, 1242
BspACI CCGC 1 cut(s) 561
BspANI GGCC 3 cut(s) 622, 903, 1203
BspCNI CTCAG 1 cut(s) 834
BspLI GGNNCC 3 cut(s) 183, 607, 1204
BspMAI CTGCAG 1 cut(s) 808
BspMI ACCTGC 1 cut(s) 881
BspPI GGATC 3 cut(s) 176, 189, 1137
BsrDI GCAATG 2 cut(s) 601, 1056
BsrI ACTGG 1 cut(s) 555
BssECI CCNNGG 5 cut(s) 252, 329, 1179, 1193, 1242
BssMI GATC 3 cut(s) 181, 508, 1129
BssNI GRCGYC 1 cut(s) 1239
BssT1I CCWWGG 3 cut(s) 252, 1193, 1242
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 5 cut(s) 403, 433, 439, 505, 1273
Bst6I CTCTTC 1 cut(s) 528
BstACI GRCGYC 1 cut(s) 1239
BstAPI GCANNNNNTGC 1 cut(s) 812
BstC8I GCNNGC 6 cut(s) 114, 321, 620, 804, 813, 948
BstDEI CTNAG 2 cut(s) 71, 842
BstDSI CCRYGG 2 cut(s) 1193, 1242
BstF5I GGATG 4 cut(s) 313, 763, 904, 1093
BstH2I RGCGCY 1 cut(s) 338
BstHHI GCGC 4 cut(s) 337, 1115, 1168, 1217
BstKTI GATC 3 cut(s) 184, 511, 1132
BstMBI GATC 3 cut(s) 181, 508, 1129
BstMWI GCNNNNNNNGC 6 cut(s) 122, 341, 706, 808, 812, 1119
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 1 cut(s) 946
BstSCI CCNGG 2 cut(s) 622, 1179
BstSFI CTRYAG 1 cut(s) 804
BstV1I GCAGC 5 cut(s) 226, 303, 602, 766, 802
BstV2I GAAGAC 2 cut(s) 733, 759
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 3 cut(s) 622, 903, 1203
BtgI CCRYGG 2 cut(s) 1193, 1242
BtsCI GGATG 4 cut(s) 313, 763, 904, 1093
BtsIMutI CAGTG 3 cut(s) 408, 562, 1278
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 6 cut(s) 114, 321, 620, 804, 813, 948
CfoI GCGC 4 cut(s) 337, 1115, 1168, 1217
Cfr13I GGNCC 2 cut(s) 605, 1202
CseI GACGC 1 cut(s) 1247
Csp6I GTAC 1 cut(s) 396
CviAII CATG 5 cut(s) 692, 789, 943, 1194, 1243
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 2 cut(s) 71, 842
DpnI GATC 3 cut(s) 183, 510, 1131
DpnII GATC 3 cut(s) 181, 508, 1129
EaeI YGGCCR 1 cut(s) 901
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
Eco130I CCWWGG 3 cut(s) 252, 1193, 1242
Eco47I GGWCC 1 cut(s) 605
Eco88I CYCGRG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 1202
EcoRI GAATTC 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 3 cut(s) 252, 1193, 1242
ErhI CCWWGG 3 cut(s) 252, 1193, 1242
FaeI CATG 5 cut(s) 695, 792, 946, 1197, 1246
FalI AAGNNNNNCTT 2 cut(s) 693, 725
FaqI GGGAC 3 cut(s) 591, 665, 867
FatI CATG 5 cut(s) 691, 788, 942, 1193, 1242
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 5 cut(s) 240, 317, 616, 780, 816
FokI GGATG 4 cut(s) 320, 770, 891, 1100
Fsp4HI GCNGC 5 cut(s) 240, 317, 616, 780, 816
FspBI CTAG 2 cut(s) 300, 642
FspI TGCGCA 2 cut(s) 1114, 1216
GlaI GCGC 4 cut(s) 336, 1114, 1167, 1216
GluI GCNGC 5 cut(s) 240, 317, 616, 780, 816
GsaI CCCAGC 1 cut(s) 723
HaeII RGCGCY 1 cut(s) 338
HaeIII GGCC 3 cut(s) 622, 903, 1203
HapII CCGG 1 cut(s) 1181
HgaI GACGC 1 cut(s) 1247
HhaI GCGC 4 cut(s) 337, 1115, 1168, 1217
Hin1I GRCGYC 1 cut(s) 1239
Hin1II CATG 5 cut(s) 695, 792, 946, 1197, 1246
Hin6I GCGC 4 cut(s) 335, 1113, 1166, 1215
HinP1I GCGC 4 cut(s) 335, 1113, 1166, 1215
HincII GTYRAC 2 cut(s) 189, 886
HindII GTYRAC 2 cut(s) 189, 886
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 3 cut(s) 52, 455, 1076
HpaII CCGG 1 cut(s) 1181
HphI GGTGA 3 cut(s) 89, 575, 665
Hpy166II GTNNAC 4 cut(s) 189, 886, 988, 1159
Hpy188I TCNGA 8 cut(s) 57, 181, 745, 772, 843, 978, 1016, 1227
Hpy188III TCNNGA 3 cut(s) 65, 459, 1085
Hpy8I GTNNAC 4 cut(s) 189, 886, 988, 1159
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 9 cut(s) 335, 567, 620, 906, 1034, 1139, 1193, 1216, 1241
HpyCH4III ACNGT 5 cut(s) 403, 433, 439, 505, 1273
HpyCH4V TGCA 6 cut(s) 319, 344, 443, 806, 818, 1091
HpyF10VI GCNNNNNNNGC 6 cut(s) 122, 341, 706, 808, 812, 1119
HpyF3I CTNAG 2 cut(s) 71, 842
Hsp92I GRCGYC 1 cut(s) 1239
Hsp92II CATG 5 cut(s) 695, 792, 946, 1197, 1246
HspAI GCGC 4 cut(s) 335, 1113, 1166, 1215
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 3 cut(s) 181, 508, 1129
LmnI GCTCC 1 cut(s) 541
Lsp1109I GCAGC 5 cut(s) 226, 303, 602, 766, 802
LweI GCATC 5 cut(s) 452, 748, 759, 1078, 1193
MaeI CTAG 2 cut(s) 300, 642
MaeIII GTNAC 4 cut(s) 403, 433, 547, 653
MalI GATC 3 cut(s) 183, 510, 1131
MboI GATC 3 cut(s) 181, 508, 1129
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 1 cut(s) 181
MlsI TGGCCA 1 cut(s) 903
MluCI AATT 6 cut(s) 171, 259, 368, 648, 927, 1093
MluNI TGGCCA 1 cut(s) 903
MlyI GAGTC 1 cut(s) 46
Mox20I TGGCCA 1 cut(s) 903
MroXI GAANNNNTTC 1 cut(s) 175
MscI TGGCCA 1 cut(s) 903
MseI TTAA 3 cut(s) 500, 831, 930
MslI CAYNNNNRTG 1 cut(s) 662
Msp20I TGGCCA 1 cut(s) 903
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 1 cut(s) 1181
MspR9I CCNGG 2 cut(s) 624, 1181
MunI CAATTG 1 cut(s) 648
MvaI CCWGG 1 cut(s) 624
MwoI GCNNNNNNNGC 6 cut(s) 122, 341, 706, 808, 812, 1119
NciI CCSGG 1 cut(s) 1181
NcoI CCATGG 2 cut(s) 1193, 1242
NdeII GATC 3 cut(s) 181, 508, 1129
NlaIII CATG 5 cut(s) 695, 792, 946, 1197, 1246
NlaIV GGNNCC 3 cut(s) 183, 607, 1204
NmuCI GTSAC 3 cut(s) 403, 433, 653
NsbI TGCGCA 2 cut(s) 1114, 1216
NspI RCATGY 1 cut(s) 946
OliI CACNNNNGTG 1 cut(s) 662
PdmI GAANNNNTTC 1 cut(s) 175
PfeI GAWTC 2 cut(s) 455, 1076
PkrI GCNGC 5 cut(s) 241, 318, 617, 781, 817
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PsiI TTATAA 2 cut(s) 152, 734
Psp6I CCWGG 1 cut(s) 622
PspFI CCCAGC 1 cut(s) 719
PspGI CCWGG 1 cut(s) 622
PspN4I GGNNCC 3 cut(s) 183, 607, 1204
PspPI GGNCC 2 cut(s) 605, 1202
PstI CTGCAG 1 cut(s) 808
PsuI RGATCY 1 cut(s) 181
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
RseI CAYNNNNRTG 1 cut(s) 662
SaqAI TTAA 3 cut(s) 500, 831, 930
SatI GCNGC 5 cut(s) 240, 317, 616, 780, 816
Sau3AI GATC 3 cut(s) 181, 508, 1129
Sau96I GGNCC 2 cut(s) 605, 1202
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 2 cut(s) 624, 1181
SfaNI GCATC 5 cut(s) 452, 748, 759, 1078, 1193
SfcI CTRYAG 1 cut(s) 804
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 291
SmoI CTYRAG 1 cut(s) 291
SpeI ACTAGT 1 cut(s) 299
Sse9I AATT 6 cut(s) 171, 259, 368, 648, 927, 1093
SsiI CCGC 1 cut(s) 561
SspI AATATT 1 cut(s) 583
SspMI CTAG 2 cut(s) 300, 642
StyD4I CCNGG 2 cut(s) 622, 1179
StyI CCWWGG 3 cut(s) 252, 1193, 1242
TaaI ACNGT 5 cut(s) 403, 433, 439, 505, 1273
TaqI TCGA 4 cut(s) 20, 460, 793, 1056
TasI AATT 6 cut(s) 171, 259, 368, 648, 927, 1093
TfiI GAWTC 2 cut(s) 455, 1076
Tru1I TTAA 3 cut(s) 500, 831, 930
Tru9I TTAA 3 cut(s) 500, 831, 930
TscAI CASTG 3 cut(s) 408, 562, 1278
TseFI GTSAC 3 cut(s) 403, 433, 653
TseI GCWGC 5 cut(s) 239, 316, 615, 779, 815
Tsp45I GTSAC 3 cut(s) 403, 433, 653
TspDTI ATGAA 5 cut(s) 41, 164, 324, 543, 1074
TspGWI ACGGA 3 cut(s) 174, 973, 1097
TspRI CASTG 3 cut(s) 408, 562, 1278
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 4 cut(s) 171, 259, 368, 1093
XceI RCATGY 1 cut(s) 946
XcmI CCANNNNNNNNNTGG 1 cut(s) 1270
XmnI GAANNNNTTC 1 cut(s) 175
XspI CTAG 2 cut(s) 300, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.