pycom12g20170

pre-mRNA-splicing factor ISY1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006322
Physical Location & Seq
Forward (+)
21772470 .. 21773680
1211 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1098 bp
ATGCAAGCGACCATTTTTTACCGTGGTGAAAATGTATTGAGCCCCTTGTATAATAACATGGTTCAAATCTCAAAAAAAATGTCCAACAGTAACAAATTTTCACGTAACAGCCACTATTTCACTCTCGAGGTTGAACAAAAAATCCCAAATTCTCCAAAACTATCTCCATCTAGGGGTTTTTTAGGGTTTAATCAAATTCCATCGACAGAAAGCGCAGTCGATGCTCAATCGATTCATTACCCTCAAAGCCGAAGAGAAGAAGAAGCCAAAAGAGCGACGCCCATACCTCGCCTCCGAGTGCCGCGATCTCGCCGAGGCCTCCAGATGGCGGCAGCAAATCATGGGGGAGATCGGCCGGAAAGTTGCCGAGATCCAGAACGAAGCCCTCGGGGAGCACCGGACCCGCGACCTCAACGACGATCGATCAACAAGCTAATTCGAGAGAAGGTCCATTGGGAGCGGCGGATTGTGGAGCTGGGAGGCCCAAATTATGCCAAAAACGCCCCTAAGATGACCGACCTCGACGGTAATATTGTCGATGTTCCAAACCCCAGTGGCCGCGGCCCAGGGTATCGGTACTTTGGAGCGGCGAAGAAGCTGCCCGGAGTCAGAGAGCTTTTCGAGAAGCCTCCGGAGCTGAGAAAGCGGAGGACGAGGTATGATATATACAAGAGGATTGATGCTAGTTATTATGGGTATAGGGACGATGAGGATGGGATTTTGGAGAAGCTCGAGGTGGAGGCGGAGGAGAAGATGAGGGCGGAGGCGGTGGCGGAGTGGAAGAGGATGGAGGAGATAAGACGTGAGGCGAGGAGGGCGGTGAAGAGCGGGGAGGTGGTGTCTGCAGCAAAGGTGAAGGAGGTTTTGTTTGAGGAGGAAGAGGAGGTGGTGGAGGAGGAGAGGCAGAGGGAGAAGGAATTGAAAGAGAGGAGTGAGCAGGAGAGGGAGTTTGTGGTGCACGTGCCGCTGCCGGATGATAAGGAGATTGAGAAGATGGTGCTGGAGAAGAAGAAGAGGGAGCTGCTGAGTAAGTATACCAGTGATGAATTGATGGAGGAGCAGACTGAGGCTAAGGAGATGCTTAACATTAAGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Isy1

Protein Analysis

366

Amino Acids

42.58

Weight (kDa)

9.14

Isoelectric Point (pI)

74.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isy1 PF06246 141 - 344 9.8e-53 Isy1-like splicing family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 460, 587, 828
AccI GTMKAC 1 cut(s) 1034
AccII CGCG 3 cut(s) 304, 406, 561
AccIII TCCGGA 1 cut(s) 631
AclWI GGATC 1 cut(s) 365
AcoI YGGCCR 2 cut(s) 353, 556
AcsI RAATTY 3 cut(s) 95, 148, 195
AcvI CACGTG 1 cut(s) 961
AcyI GRCGYC 1 cut(s) 278
AfaI GTAC 1 cut(s) 578
AfeI AGCGCT 1 cut(s) 1094
AfiI CCNNNNNNNGG 3 cut(s) 173, 325, 328
AgsI TTSAA 3 cut(s) 65, 134, 922
AjiI CACGTC 1 cut(s) 803
AjnI CCWGG 1 cut(s) 565
AloI GAACNNNNNNTCC 2 cut(s) 126, 158
AluBI AGCT 7 cut(s) 433, 475, 598, 616, 637, 730, 1021
AluI AGCT 7 cut(s) 433, 475, 598, 616, 637, 730, 1021
Alw21I GWGCWC 2 cut(s) 397, 960
Alw44I GTGCAC 1 cut(s) 956
AlwI GGATC 1 cut(s) 365
Ama87I CYCGRG 3 cut(s) 125, 387, 731
Aor13HI TCCGGA 1 cut(s) 631
Aor51HI AGCGCT 1 cut(s) 1094
AoxI GGCC 5 cut(s) 316, 353, 481, 556, 562
ApaLI GTGCAC 1 cut(s) 956
ApeKI GCWGC 5 cut(s) 332, 598, 845, 967, 1021
ApoI RAATTY 3 cut(s) 95, 148, 195
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AspLEI GCGC 2 cut(s) 215, 1095
AspS9I GGNCC 4 cut(s) 400, 448, 482, 563
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 3 cut(s) 38, 832, 865
AvaI CYCGRG 3 cut(s) 125, 387, 731
AvaII GGWCC 2 cut(s) 400, 448
BaeGI GKGCMC 1 cut(s) 960
BanII GRGCYC 1 cut(s) 44
BbrPI CACGTG 1 cut(s) 961
Bbv12I GWGCWC 2 cut(s) 397, 960
BbvI GCAGC 5 cut(s) 344, 585, 857, 954, 1008
BccI CCATC 7 cut(s) 175, 208, 319, 707, 781, 988, 1045
BcgI CGANNNNNNTGC 2 cut(s) 580, 614
BciT130I CCWGG 1 cut(s) 567
BcnI CCSGG 1 cut(s) 603
BfaI CTAG 3 cut(s) 171, 684, 1096
BfmI CTRYAG 1 cut(s) 843
BfoI RGCGCY 1 cut(s) 1096
Bme1390I CCNGG 2 cut(s) 567, 603
Bme18I GGWCC 2 cut(s) 400, 448
BmeT110I CYCGRG 3 cut(s) 125, 387, 731
BmgBI CACGTC 1 cut(s) 803
BmgT120I GGNCC 4 cut(s) 400, 448, 482, 563
BmiI GGNNCC 1 cut(s) 402
BmrFI CCNGG 2 cut(s) 567, 603
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 3 cut(s) 211, 670, 1068
BmuI ACTGGG 1 cut(s) 546
BpmI CTGGAG 2 cut(s) 305, 1022
Bpu10I CCTNAGC 1 cut(s) 1071
BpuMI CCSGG 1 cut(s) 603
Bsa29I ATCGAT 2 cut(s) 230, 422
BsaAI YACGTR 2 cut(s) 104, 961
BsaHI GRCGYC 1 cut(s) 278
BsaJI CCNNGG 6 cut(s) 22, 313, 386, 559, 565, 566
BsaWI WCCGGW 2 cut(s) 397, 631
BsaXI ACNNNNNCTCC 8 cut(s) 276, 306, 824, 851, 854, 881, 932, 962
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 325, 328
Bse1I ACTGG 2 cut(s) 552, 1038
BseAI TCCGGA 1 cut(s) 631
BseBI CCWGG 1 cut(s) 567
BseCI ATCGAT 2 cut(s) 230, 422
BseDI CCNNGG 6 cut(s) 22, 313, 386, 559, 565, 566
BseGI GGATG 3 cut(s) 718, 792, 979
BseLI CCNNNNNNNGG 3 cut(s) 173, 325, 328
BseMII CTCAG 3 cut(s) 629, 1016, 1056
BseNI ACTGG 2 cut(s) 552, 1038
BseRI GAGGAG 9 cut(s) 761, 806, 826, 887, 896, 908, 911, 943, 1070
BseSI GKGCMC 1 cut(s) 960
BseX3I CGGCCG 1 cut(s) 353
BseXI GCAGC 5 cut(s) 344, 585, 857, 954, 1008
BseYI CCCAGC 1 cut(s) 475
Bsh1236I CGCG 3 cut(s) 304, 406, 561
Bsh1285I CGRYCG 2 cut(s) 356, 422
BshFI GGCC 5 cut(s) 318, 355, 483, 558, 564
BshVI ATCGAT 2 cut(s) 230, 422
BsiEI CGRYCG 2 cut(s) 356, 422
BsiHKAI GWGCWC 2 cut(s) 397, 960
BsiHKCI CYCGRG 3 cut(s) 125, 387, 731
BsiSI CCGG 5 cut(s) 356, 398, 603, 632, 971
BslFI GGGAC 1 cut(s) 716
BslI CCNNNNNNNGG 3 cut(s) 173, 325, 328
BsmFI GGGAC 1 cut(s) 716
BsnI GGCC 5 cut(s) 318, 355, 483, 558, 564
BsoBI CYCGRG 3 cut(s) 125, 387, 731
Bsp1286I GDGCHC 3 cut(s) 44, 397, 960
Bsp13I TCCGGA 1 cut(s) 631
Bsp143I GATC 5 cut(s) 305, 349, 370, 419, 423
BspANI GGCC 5 cut(s) 318, 355, 483, 558, 564
BspCNI CTCAG 3 cut(s) 630, 1017, 1057
BspDI ATCGAT 2 cut(s) 230, 422
BspEI TCCGGA 1 cut(s) 631
BspFNI CGCG 3 cut(s) 304, 406, 561
BspLI GGNNCC 1 cut(s) 402
BspMAI CTGCAG 1 cut(s) 847
BspPI GGATC 1 cut(s) 365
BspQI GCTCTTC 1 cut(s) 818
BsrBI CCGCTC 3 cut(s) 460, 587, 828
BsrI ACTGG 2 cut(s) 552, 1038
BssECI CCNNGG 6 cut(s) 22, 313, 386, 559, 565, 566
BssMI GATC 5 cut(s) 305, 349, 370, 419, 423
BssNAI GTATAC 1 cut(s) 1035
BssNI GRCGYC 1 cut(s) 278
Bst1107I GTATAC 1 cut(s) 1035
Bst2UI CCWGG 1 cut(s) 567
Bst4CI ACNGT 3 cut(s) 23, 89, 527
Bst6I CTCTTC 5 cut(s) 247, 776, 818, 873, 1007
BstACI GRCGYC 1 cut(s) 278
BstAPI GCANNNNNTGC 1 cut(s) 221
BstBAI YACGTR 2 cut(s) 104, 961
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 5 cut(s) 507, 638, 1025, 1065, 1071
BstDSI CCRYGG 2 cut(s) 22, 559
BstF5I GGATG 3 cut(s) 718, 792, 979
BstFNI CGCG 3 cut(s) 304, 406, 561
BstH2I RGCGCY 1 cut(s) 1096
BstHHI GCGC 2 cut(s) 215, 1095
BstKTI GATC 5 cut(s) 308, 352, 373, 422, 426
BstMBI GATC 5 cut(s) 305, 349, 370, 419, 423
BstMCI CGRYCG 2 cut(s) 356, 422
BstMWI GCNNNNNNNGC 7 cut(s) 221, 272, 500, 634, 643, 815, 964
BstNI CCWGG 1 cut(s) 567
BstSCI CCNGG 2 cut(s) 565, 601
BstSFI CTRYAG 1 cut(s) 843
BstSLI GKGCMC 1 cut(s) 960
BstUI CGCG 3 cut(s) 304, 406, 561
BstV1I GCAGC 5 cut(s) 344, 585, 857, 954, 1008
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BstZ17I GTATAC 1 cut(s) 1035
BstZI CGGCCG 1 cut(s) 353
Bsu15I ATCGAT 2 cut(s) 230, 422
BsuRI GGCC 5 cut(s) 318, 355, 483, 558, 564
BsuTUI ATCGAT 2 cut(s) 230, 422
BtgI CCRYGG 2 cut(s) 22, 559
BtrI CACGTC 1 cut(s) 803
BtsCI GGATG 3 cut(s) 718, 792, 979
BtsIMutI CAGTG 2 cut(s) 559, 1045
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 2 cut(s) 215, 1095
Cfr13I GGNCC 4 cut(s) 400, 448, 482, 563
Cfr42I CCGCGG 1 cut(s) 562
ClaI ATCGAT 2 cut(s) 230, 422
CseI GACGC 1 cut(s) 286
Csp6I GTAC 1 cut(s) 577
CviAII CATG 2 cut(s) 58, 341
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 5 cut(s) 507, 638, 1025, 1065, 1071
DpnI GATC 5 cut(s) 307, 351, 372, 421, 425
DpnII GATC 5 cut(s) 305, 349, 370, 419, 423
EaeI YGGCCR 2 cut(s) 353, 556
EagI CGGCCG 1 cut(s) 353
Eam1104I CTCTTC 5 cut(s) 247, 776, 818, 873, 1007
EarI CTCTTC 5 cut(s) 247, 776, 818, 873, 1007
EciI GGCGGA 4 cut(s) 478, 758, 776, 788
EclXI CGGCCG 1 cut(s) 353
Eco147I AGGCCT 1 cut(s) 318
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 2 cut(s) 400, 448
Eco47III AGCGCT 1 cut(s) 1094
Eco52I CGGCCG 1 cut(s) 353
Eco72I CACGTG 1 cut(s) 961
Eco88I CYCGRG 3 cut(s) 125, 387, 731
EcoRII CCWGG 1 cut(s) 565
EcoT38I GRGCYC 1 cut(s) 44
FaeI CATG 2 cut(s) 61, 344
FaqI GGGAC 1 cut(s) 716
FatI CATG 2 cut(s) 57, 340
FauI CCCGC 2 cut(s) 411, 821
FblI GTMKAC 1 cut(s) 1034
FokI GGATG 3 cut(s) 725, 799, 986
FriOI GRGCYC 1 cut(s) 44
FspBI CTAG 3 cut(s) 171, 684, 1096
GlaI GCGC 2 cut(s) 214, 1094
GsaI CCCAGC 1 cut(s) 479
GsuI CTGGAG 2 cut(s) 305, 1022
HaeII RGCGCY 1 cut(s) 1096
HaeIII GGCC 5 cut(s) 318, 355, 483, 558, 564
HapII CCGG 5 cut(s) 356, 398, 603, 632, 971
HgaI GACGC 1 cut(s) 286
HhaI GCGC 2 cut(s) 215, 1095
Hin1I GRCGYC 1 cut(s) 278
Hin1II CATG 2 cut(s) 61, 344
Hin6I GCGC 2 cut(s) 213, 1093
HinP1I GCGC 2 cut(s) 213, 1093
HinfI GANTC 2 cut(s) 232, 606
HpaII CCGG 5 cut(s) 356, 398, 603, 632, 971
HphI GGTGA 3 cut(s) 38, 832, 865
Hpy166II GTNNAC 2 cut(s) 958, 1035
Hpy188I TCNGA 2 cut(s) 296, 611
Hpy188III TCNNGA 6 cut(s) 125, 322, 374, 440, 622, 632
Hpy8I GTNNAC 2 cut(s) 958, 1035
Hpy99I CGWCG 3 cut(s) 280, 420, 527
HpyAV CCTTC 3 cut(s) 439, 850, 907
HpyCH4III ACNGT 3 cut(s) 23, 89, 527
HpyCH4IV ACGT 3 cut(s) 103, 802, 960
HpyCH4V TGCA 3 cut(s) 4, 845, 958
HpyF10VI GCNNNNNNNGC 7 cut(s) 221, 272, 500, 634, 643, 815, 964
HpyF3I CTNAG 5 cut(s) 507, 638, 1025, 1065, 1071
HpySE526I ACGT 3 cut(s) 103, 802, 960
Hsp92I GRCGYC 1 cut(s) 278
Hsp92II CATG 2 cut(s) 61, 344
HspAI GCGC 2 cut(s) 213, 1093
Kpn2I TCCGGA 1 cut(s) 631
KspI CCGCGG 1 cut(s) 562
Kzo9I GATC 5 cut(s) 305, 349, 370, 419, 423
LguI GCTCTTC 1 cut(s) 818
LmnI GCTCC 7 cut(s) 392, 457, 472, 584, 634, 1018, 1057
Lsp1109I GCAGC 5 cut(s) 344, 585, 857, 954, 1008
LweI GCATC 3 cut(s) 211, 670, 1068
MaeI CTAG 3 cut(s) 171, 684, 1096
MaeII ACGT 3 cut(s) 103, 802, 960
MaeIII GTNAC 2 cut(s) 89, 104
MalI GATC 5 cut(s) 307, 351, 372, 421, 425
MbiI CCGCTC 3 cut(s) 460, 587, 828
MboI GATC 5 cut(s) 305, 349, 370, 419, 423
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 3 cut(s) 44, 397, 960
MluCI AATT 7 cut(s) 95, 148, 195, 435, 487, 917, 1046
MlyI GAGTC 1 cut(s) 615
MmeI TCCRAC 1 cut(s) 108
MroI TCCGGA 1 cut(s) 631
MseI TTAA 3 cut(s) 189, 1083, 1089
MspA1I CMGCKG 2 cut(s) 561, 967
MspI CCGG 5 cut(s) 356, 398, 603, 632, 971
MspR9I CCNGG 2 cut(s) 567, 603
MvaI CCWGG 1 cut(s) 567
MvnI CGCG 3 cut(s) 304, 406, 561
MwoI GCNNNNNNNGC 7 cut(s) 221, 272, 500, 634, 643, 815, 964
NciI CCSGG 1 cut(s) 603
NdeII GATC 5 cut(s) 305, 349, 370, 419, 423
NlaIII CATG 2 cut(s) 61, 344
NlaIV GGNNCC 1 cut(s) 402
NmeAIII GCCGAG 2 cut(s) 338, 392
PaeR7I CTCGAG 2 cut(s) 125, 731
PasI CCCWGGG 1 cut(s) 566
PceI AGGCCT 1 cut(s) 318
PciSI GCTCTTC 1 cut(s) 818
PfeI GAWTC 1 cut(s) 232
Ple19I CGATCG 1 cut(s) 422
PleI GAGTC 1 cut(s) 614
PmaCI CACGTG 1 cut(s) 961
PmlI CACGTG 1 cut(s) 961
PpsI GAGTC 1 cut(s) 614
Ppu21I YACGTR 2 cut(s) 104, 961
Psp6I CCWGG 1 cut(s) 565
PspCI CACGTG 1 cut(s) 961
PspFI CCCAGC 1 cut(s) 475
PspGI CCWGG 1 cut(s) 565
PspN4I GGNNCC 1 cut(s) 402
PspPI GGNCC 4 cut(s) 400, 448, 482, 563
PspXI VCTCGAGB 1 cut(s) 731
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 370
PvuI CGATCG 1 cut(s) 422
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
SacII CCGCGG 1 cut(s) 562
SapI GCTCTTC 1 cut(s) 818
SaqAI TTAA 3 cut(s) 189, 1083, 1089
Sau3AI GATC 5 cut(s) 305, 349, 370, 419, 423
Sau96I GGNCC 4 cut(s) 400, 448, 482, 563
SchI GAGTC 1 cut(s) 615
ScrFI CCNGG 2 cut(s) 567, 603
SduI GDGCHC 3 cut(s) 44, 397, 960
SfaNI GCATC 3 cut(s) 211, 670, 1068
SfcI CTRYAG 1 cut(s) 843
Sfr274I CTCGAG 2 cut(s) 125, 731
Sfr303I CCGCGG 1 cut(s) 562
SgrBI CCGCGG 1 cut(s) 562
SinI GGWCC 2 cut(s) 400, 448
SlaI CTCGAG 2 cut(s) 125, 731
SmlI CTYRAG 2 cut(s) 125, 731
SmoI CTYRAG 2 cut(s) 125, 731
Sse9I AATT 7 cut(s) 95, 148, 195, 435, 487, 917, 1046
SseBI AGGCCT 1 cut(s) 318
SspI AATATT 1 cut(s) 532
SspMI CTAG 3 cut(s) 171, 684, 1096
StuI AGGCCT 1 cut(s) 318
StyD4I CCNGG 2 cut(s) 565, 601
TaaI ACNGT 3 cut(s) 23, 89, 527
TaiI ACGT 3 cut(s) 106, 805, 963
TaqII GACCGA 1 cut(s) 530
TasI AATT 7 cut(s) 95, 148, 195, 435, 487, 917, 1046
TauI GCSGC 7 cut(s) 304, 332, 463, 561, 564, 590, 967
TfiI GAWTC 1 cut(s) 232
Tru1I TTAA 3 cut(s) 189, 1083, 1089
Tru9I TTAA 3 cut(s) 189, 1083, 1089
TscAI CASTG 2 cut(s) 559, 1045
TseI GCWGC 5 cut(s) 332, 598, 845, 967, 1021
TspDTI ATGAA 2 cut(s) 224, 1059
TspRI CASTG 2 cut(s) 559, 1045
VneI GTGCAC 1 cut(s) 956
VpaK11BI GGWCC 2 cut(s) 400, 448
XapI RAATTY 3 cut(s) 95, 148, 195
XhoI CTCGAG 2 cut(s) 125, 731
XmiI GTMKAC 1 cut(s) 1034
XspI CTAG 3 cut(s) 171, 684, 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.