pycom13g08570

Ribose-phosphate pyrophosphokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007309
Physical Location & Seq
Reverse (-)
5702578 .. 5705209
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g08570.5

Sequence Viewer

Length: 1227 bp
ATGGCGGCCACTCGAGGCCTCCCGTCTTCCTTCCAAAACCCTGCCTTCCCAGCCGCGCCCAAATGTCCCATTCCTTCCTCCGCCGCTTTCCATATCAAAGCCTCCACCTTTACGAAGAAGAAGCAGTACAGCAGCAACATCATCAGGTGCGACATCAAGAGCTTCGGCCACCCGCACAACCTGACCATCGAGCGGGACTCTCCGGTTCACGTCTTCCGCGATGCTTCCGAAGCCCAATTGTCAATGGCATCCGATTCGGCAGGGAAGAAGGCGAAGAAGGTCTGCCTTTTCTACTGCGCCGAGACCAAAGCCCTCGCCCAGAACGTCGCCGCCCAGTCGGACTCCATCGTGCTCCGCAGTATCACGTGGAGGAAATTTGACGATGGGTTCCCCAACATTTTCATACCCAATGCTCATGGAATTCGAGGGCATCATGTGGCGTTTCTGGCCTCGTTTAGCTCCCCGTCGGTCATTTTCGAGCAGTTATCTGTGATTTATGCATTGCCCAAGTTGTTCATCTCCTCATTCACTCTGGTGCTTCCATTTTTTCCGACGGGGACTTCAGAGCGTATGGAGGATGAGGGAGATGTCGCCACTGCTTTCACGCTCGCCAGGGCATTGTCGAACATTCCCATTTCGATGGGAGGGCCTACTAGTTTAGTCATCTATGATATCCATGCCTTGCAGGAGCGGTTTTACTTTGGAGATAATGTCTTACCGTGCTTTGAGAGCGGGATCCCTTTGCTCAAAAATAGGCTTCAGCAGCTCCCTGATTCCGACAATATATCCATTGCTTTTCCTGATGATGGTGCATGGAAACGATTTCATAAGCAGCTGCATCATTTCCCAACAATTGTCTGTGCTAAAGTTCGGGAAGGTGACCAACGAATCGTGCGTATTAAAGAGGGAGATCCTAGAGGACGACATGTTGTGATTGTTGATGATTTGGTTCAATCTGGTGGTACCTTGATCGAATGCCAGAAAGTTTTAGCCGCCCATGGAGCAGCAAAAATCAGTGCTTATGTAACACACGGGATCTTTCCTAATCGATCATGGGAACGCTTTCAAAGTGATTACGGAGGGCACCCCGAGCATGGGCTGACCTACTTCTGGATTACGGACTCGTGTCCACTGACAGTGAAAGATGTGAAGAACAATCCACCATTTGAAGTTCTTAGCCTTGCTGGGTCCATAGCGACTACTCTTCAAATGATGGCCCATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pribosyltran Pribosyltran_N

Protein Analysis

409

Amino Acids

45.03

Weight (kDa)

8.35

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 962
AccB1I GGYRCC 2 cut(s) 962, 1083
AccBSI CCGCTC 3 cut(s) 193, 691, 732
AccII CGCG 2 cut(s) 56, 219
AclWI GGATC 4 cut(s) 730, 743, 905, 1043
AcoI YGGCCR 2 cut(s) 6, 166
AcsI RAATTY 2 cut(s) 374, 420
AcuI CTGAAG 2 cut(s) 546, 743
AcvI CACGTG 1 cut(s) 366
AfaI GTAC 2 cut(s) 128, 964
AfiI CCNNNNNNNGG 5 cut(s) 192, 806, 1094, 1095, 1110
AflIII ACRYGT 1 cut(s) 925
AgsI TTSAA 4 cut(s) 953, 1067, 1169, 1208
AhlI ACTAGT 1 cut(s) 653
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 1 cut(s) 611
AleI CACNNNNGTG 1 cut(s) 533
AluBI AGCT 4 cut(s) 162, 459, 766, 835
AluI AGCT 4 cut(s) 162, 459, 766, 835
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 4 cut(s) 730, 743, 905, 1043
Ama87I CYCGRG 2 cut(s) 12, 1088
AoxI GGCC 6 cut(s) 6, 16, 166, 447, 647, 1215
ApeKI GCWGC 5 cut(s) 132, 763, 832, 835, 1004
ApoI RAATTY 2 cut(s) 374, 420
Asp700I GAANNNNTTC 1 cut(s) 1062
Asp718I GGTACC 1 cut(s) 962
AspLEI GCGC 2 cut(s) 58, 299
AspS9I GGNCC 3 cut(s) 647, 1188, 1216
AsuHPI GGTGA 1 cut(s) 890
AvaI CYCGRG 2 cut(s) 12, 1088
AvaII GGWCC 1 cut(s) 1188
BaeGI GKGCMC 1 cut(s) 1086
BamHI GGATCC 1 cut(s) 735
BanI GGYRCC 2 cut(s) 962, 1083
BarI GAAGNNNNNNTAC 2 cut(s) 110, 142
BauI CACGAG 1 cut(s) 1123
BbrPI CACGTG 1 cut(s) 366
BbsI GAAGAC 2 cut(s) 18, 205
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 5 cut(s) 144, 775, 822, 844, 1016
BccI CCATC 6 cut(s) 194, 353, 377, 634, 800, 1207
BcgI CGANNNNNNTGC 2 cut(s) 237, 271
BciT130I CCWGG 1 cut(s) 613
BcoDI GTCTC 1 cut(s) 296
BcuI ACTAGT 1 cut(s) 653
BfaI CTAG 2 cut(s) 654, 915
Bme1390I CCNGG 1 cut(s) 613
Bme18I GGWCC 1 cut(s) 1188
BmeT110I CYCGRG 2 cut(s) 12, 1088
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 3 cut(s) 647, 1188, 1216
BmiI GGNNCC 5 cut(s) 389, 737, 964, 1085, 1189
BmrFI CCNGG 1 cut(s) 613
BmrI ACTGGG 1 cut(s) 328
BmsI GCATC 4 cut(s) 211, 257, 439, 847
BmuI ACTGGG 1 cut(s) 328
BoxI GACNNNNGTC 1 cut(s) 1125
BpiI GAAGAC 2 cut(s) 18, 205
BplI GAGNNNNNCTC 2 cut(s) 182, 214
Bsa29I ATCGAT 1 cut(s) 1048
BsaAI YACGTR 1 cut(s) 366
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 2 cut(s) 612, 997
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 5 cut(s) 192, 806, 1094, 1095, 1110
Bse1I ACTGG 1 cut(s) 334
Bse3DI GCAATG 2 cut(s) 500, 789
BseBI CCWGG 1 cut(s) 613
BseCI ATCGAT 1 cut(s) 1048
BseDI CCNNGG 2 cut(s) 612, 997
BseGI GGATG 2 cut(s) 248, 583
BseLI CCNNNNNNNGG 5 cut(s) 192, 806, 1094, 1095, 1110
BseMI GCAATG 2 cut(s) 500, 789
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 1 cut(s) 511
BseSI GKGCMC 1 cut(s) 1086
BseXI GCAGC 5 cut(s) 144, 775, 822, 844, 1016
BseYI CCCAGC 2 cut(s) 49, 1184
Bsh1236I CGCG 2 cut(s) 56, 219
BshFI GGCC 6 cut(s) 8, 18, 168, 449, 649, 1217
BshNI GGYRCC 2 cut(s) 962, 1083
BshVI ATCGAT 1 cut(s) 1048
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 2 cut(s) 12, 1088
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 3 cut(s) 51, 209, 571
BslI CCNNNNNNNGG 5 cut(s) 192, 806, 1094, 1095, 1110
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 3 cut(s) 51, 209, 571
BsmI GAATGC 1 cut(s) 980
BsnI GGCC 6 cut(s) 8, 18, 168, 449, 649, 1217
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 2 cut(s) 12, 1088
Bsp1286I GDGCHC 2 cut(s) 354, 1086
Bsp143I GATC 5 cut(s) 735, 910, 969, 1035, 1049
Bsp19I CCATGG 1 cut(s) 997
BspANI GGCC 6 cut(s) 8, 18, 168, 449, 649, 1217
BspDI ATCGAT 1 cut(s) 1048
BspFNI CGCG 2 cut(s) 56, 219
BspLI GGNNCC 5 cut(s) 389, 737, 964, 1085, 1189
BspPI GGATC 4 cut(s) 730, 743, 905, 1043
BspT107I GGYRCC 2 cut(s) 962, 1083
BspTNI GGTCTC 1 cut(s) 296
BsrBI CCGCTC 3 cut(s) 193, 691, 732
BsrDI GCAATG 2 cut(s) 500, 789
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 2 cut(s) 612, 997
BssMI GATC 5 cut(s) 735, 910, 969, 1035, 1049
BssSI CACGAG 1 cut(s) 1123
BssT1I CCWWGG 1 cut(s) 997
Bst2BI CACGAG 1 cut(s) 1123
Bst2UI CCWGG 1 cut(s) 613
Bst4CI ACNGT 2 cut(s) 720, 1138
Bst6I CTCTTC 1 cut(s) 1209
BstBAI YACGTR 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 609
BstDEI CTNAG 1 cut(s) 1175
BstDSI CCRYGG 1 cut(s) 997
BstEII GGTNACC 1 cut(s) 878
BstF5I GGATG 2 cut(s) 248, 583
BstFNI CGCG 2 cut(s) 56, 219
BstHHI GCGC 2 cut(s) 58, 299
BstKTI GATC 5 cut(s) 738, 913, 972, 1038, 1052
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 5 cut(s) 735, 910, 969, 1035, 1049
BstMWI GCNNNNNNNGC 7 cut(s) 50, 230, 446, 729, 763, 1001, 1090
BstNI CCWGG 1 cut(s) 613
BstNSI RCATGY 1 cut(s) 929
BstPAI GACNNNNGTC 1 cut(s) 1125
BstPI GGTNACC 1 cut(s) 878
BstSCI CCNGG 1 cut(s) 611
BstSLI GKGCMC 1 cut(s) 1086
BstUI CGCG 2 cut(s) 56, 219
BstV1I GCAGC 5 cut(s) 144, 775, 822, 844, 1016
BstV2I GAAGAC 2 cut(s) 18, 205
BstX2I RGATCY 3 cut(s) 735, 910, 1035
BstXI CCANNNNNNTGG 1 cut(s) 640
BstYI RGATCY 3 cut(s) 735, 910, 1035
Bsu15I ATCGAT 1 cut(s) 1048
BsuRI GGCC 6 cut(s) 8, 18, 168, 449, 649, 1217
BsuTUI ATCGAT 1 cut(s) 1048
BtgI CCRYGG 1 cut(s) 997
BtgZI GCGATG 1 cut(s) 234
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 2 cut(s) 248, 583
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 4 cut(s) 594, 1021, 1130, 1143
Cac8I GCNNGC 1 cut(s) 609
CfoI GCGC 2 cut(s) 58, 299
Cfr13I GGNCC 3 cut(s) 647, 1188, 1216
ClaI ATCGAT 1 cut(s) 1048
Csp6I GTAC 2 cut(s) 127, 963
CviAII CATG 9 cut(s) 416, 434, 677, 813, 926, 998, 1053, 1094, 1220
CviQI GTAC 2 cut(s) 127, 963
DdeI CTNAG 1 cut(s) 1175
DpnI GATC 5 cut(s) 737, 912, 971, 1037, 1051
DpnII GATC 5 cut(s) 735, 910, 969, 1035, 1049
EaeI YGGCCR 2 cut(s) 6, 166
Eam1104I CTCTTC 1 cut(s) 1209
EarI CTCTTC 1 cut(s) 1209
EciI GGCGGA 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 997
Eco147I AGGCCT 1 cut(s) 18
Eco31I GGTCTC 1 cut(s) 296
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 1 cut(s) 1188
Eco57I CTGAAG 2 cut(s) 546, 743
Eco72I CACGTG 1 cut(s) 366
Eco88I CYCGRG 2 cut(s) 12, 1088
Eco91I GGTNACC 1 cut(s) 878
EcoO109I RGGNCCY 1 cut(s) 647
EcoO65I GGTNACC 1 cut(s) 878
EcoRI GAATTC 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 611
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 997
EcoT22I ATGCAT 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 997
FaeI CATG 9 cut(s) 419, 437, 680, 816, 929, 1001, 1056, 1097, 1223
FaqI GGGAC 3 cut(s) 51, 209, 571
FatI CATG 9 cut(s) 415, 433, 676, 812, 925, 997, 1052, 1093, 1219
FauI CCCGC 3 cut(s) 180, 186, 725
FokI GGATG 2 cut(s) 235, 590
FspBI CTAG 2 cut(s) 654, 915
GlaI GCGC 2 cut(s) 57, 298
GsaI CCCAGC 2 cut(s) 53, 1188
HaeIII GGCC 6 cut(s) 8, 18, 168, 449, 649, 1217
HapII CCGG 1 cut(s) 203
HhaI GCGC 2 cut(s) 58, 299
Hin1II CATG 9 cut(s) 419, 437, 680, 816, 929, 1001, 1056, 1097, 1223
Hin6I GCGC 2 cut(s) 56, 297
HinP1I GCGC 2 cut(s) 56, 297
HinfI GANTC 6 cut(s) 197, 254, 341, 773, 888, 1121
HpaII CCGG 1 cut(s) 203
HphI GGTGA 1 cut(s) 890
Hpy166II GTNNAC 2 cut(s) 208, 1130
Hpy188I TCNGA 6 cut(s) 229, 253, 340, 552, 565, 778
Hpy188III TCNNGA 4 cut(s) 157, 800, 872, 1111
Hpy8I GTNNAC 2 cut(s) 208, 1130
Hpy99I CGWCG 3 cut(s) 329, 469, 556
HpyAV CCTTC 6 cut(s) 40, 55, 84, 262, 271, 869
HpyCH4III ACNGT 2 cut(s) 720, 1138
HpyCH4IV ACGT 3 cut(s) 210, 324, 365
HpyCH4V TGCA 4 cut(s) 500, 685, 812, 838
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 230, 446, 729, 763, 1001, 1090
HpyF3I CTNAG 1 cut(s) 1175
HpySE526I ACGT 3 cut(s) 210, 324, 365
Hsp92II CATG 9 cut(s) 419, 437, 680, 816, 929, 1001, 1056, 1097, 1223
HspAI GCGC 2 cut(s) 56, 297
KpnI GGTACC 1 cut(s) 966
Kzo9I GATC 5 cut(s) 735, 910, 969, 1035, 1049
LmnI GCTCC 5 cut(s) 357, 464, 688, 771, 1001
Lsp1109I GCAGC 5 cut(s) 144, 775, 822, 844, 1016
LweI GCATC 4 cut(s) 211, 257, 439, 847
MaeI CTAG 2 cut(s) 654, 915
MaeII ACGT 3 cut(s) 210, 324, 365
MaeIII GTNAC 2 cut(s) 878, 1024
MalI GATC 5 cut(s) 737, 912, 971, 1037, 1051
MbiI CCGCTC 3 cut(s) 193, 691, 732
MboI GATC 5 cut(s) 735, 910, 969, 1035, 1049
MboII GAAGA 8 cut(s) 18, 127, 130, 205, 277, 286, 1162, 1196
MfeI CAATTG 2 cut(s) 236, 852
MflI RGATCY 3 cut(s) 735, 910, 1035
MhlI GDGCHC 2 cut(s) 354, 1086
MluCI AATT 4 cut(s) 236, 374, 420, 852
MlyI GAGTC 3 cut(s) 191, 335, 1115
MmeI TCCRAC 3 cut(s) 318, 575, 801
Mph1103I ATGCAT 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 1062
MseI TTAA 1 cut(s) 900
MslI CAYNNNNRTG 2 cut(s) 533, 638
MspA1I CMGCKG 1 cut(s) 835
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 613
MunI CAATTG 2 cut(s) 236, 852
Mva1269I GAATGC 1 cut(s) 980
MvaI CCWGG 1 cut(s) 613
MvnI CGCG 2 cut(s) 56, 219
MwoI GCNNNNNNNGC 7 cut(s) 50, 230, 446, 729, 763, 1001, 1090
NcoI CCATGG 1 cut(s) 997
NdeII GATC 5 cut(s) 735, 910, 969, 1035, 1049
NlaIII CATG 9 cut(s) 419, 437, 680, 816, 929, 1001, 1056, 1097, 1223
NlaIV GGNNCC 5 cut(s) 389, 737, 964, 1085, 1189
NmeAIII GCCGAG 1 cut(s) 325
NmuCI GTSAC 1 cut(s) 878
NsiI ATGCAT 1 cut(s) 502
NspI RCATGY 1 cut(s) 929
OliI CACNNNNGTG 1 cut(s) 533
PaeR7I CTCGAG 1 cut(s) 12
PceI AGGCCT 1 cut(s) 18
PciI ACATGT 1 cut(s) 925
PcsI WCGNNNNNNNCGW 3 cut(s) 216, 321, 892
PctI GAATGC 1 cut(s) 980
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 3 cut(s) 254, 773, 888
PleI GAGTC 3 cut(s) 191, 335, 1115
PmaCI CACGTG 1 cut(s) 366
PmlI CACGTG 1 cut(s) 366
PpsI GAGTC 3 cut(s) 191, 335, 1115
Ppu21I YACGTR 1 cut(s) 366
PscI ACATGT 1 cut(s) 925
PshAI GACNNNNGTC 1 cut(s) 1125
Psp6I CCWGG 1 cut(s) 611
PspCI CACGTG 1 cut(s) 366
PspEI GGTNACC 1 cut(s) 878
PspFI CCCAGC 2 cut(s) 49, 1184
PspGI CCWGG 1 cut(s) 611
PspN4I GGNNCC 5 cut(s) 389, 737, 964, 1085, 1189
PspPI GGNCC 3 cut(s) 647, 1188, 1216
PspXI VCTCGAGB 1 cut(s) 12
PsuI RGATCY 3 cut(s) 735, 910, 1035
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 2 cut(s) 128, 964
RsaNI GTAC 2 cut(s) 127, 963
RseI CAYNNNNRTG 2 cut(s) 533, 638
SaqAI TTAA 1 cut(s) 900
Sau3AI GATC 5 cut(s) 735, 910, 969, 1035, 1049
Sau96I GGNCC 3 cut(s) 647, 1188, 1216
SchI GAGTC 3 cut(s) 191, 335, 1115
ScrFI CCNGG 1 cut(s) 613
SduI GDGCHC 2 cut(s) 354, 1086
SfaNI GCATC 4 cut(s) 211, 257, 439, 847
Sfr274I CTCGAG 1 cut(s) 12
SinI GGWCC 1 cut(s) 1188
SlaI CTCGAG 1 cut(s) 12
SmiMI CAYNNNNRTG 2 cut(s) 533, 638
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
SpeI ACTAGT 1 cut(s) 653
Sse9I AATT 4 cut(s) 236, 374, 420, 852
SseBI AGGCCT 1 cut(s) 18
SspMI CTAG 2 cut(s) 654, 915
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 611
StyI CCWWGG 1 cut(s) 997
TaaI ACNGT 2 cut(s) 720, 1138
TaiI ACGT 3 cut(s) 213, 327, 368
TaqI TCGA 8 cut(s) 13, 189, 424, 477, 623, 638, 972, 1048
TaqII GACCGA 1 cut(s) 457
TasI AATT 4 cut(s) 236, 374, 420, 852
TatI WGTACW 1 cut(s) 126
TauI GCSGC 5 cut(s) 8, 56, 86, 332, 995
TfiI GAWTC 3 cut(s) 254, 773, 888
Tru1I TTAA 1 cut(s) 900
Tru9I TTAA 1 cut(s) 900
TscAI CASTG 4 cut(s) 601, 1021, 1137, 1143
TseFI GTSAC 1 cut(s) 878
TseI GCWGC 5 cut(s) 132, 763, 832, 835, 1004
Tsp45I GTSAC 1 cut(s) 878
TspDTI ATGAA 3 cut(s) 391, 505, 815
TspGWI ACGGA 2 cut(s) 1092, 1133
TspRI CASTG 4 cut(s) 601, 1021, 1137, 1143
VpaK11BI GGWCC 1 cut(s) 1188
XapI RAATTY 2 cut(s) 374, 420
XceI RCATGY 1 cut(s) 929
XhoI CTCGAG 1 cut(s) 12
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 2 cut(s) 654, 915
Zsp2I ATGCAT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.