Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0007309

Physical Location & Seq

Genomic Coordinates Reverse (-)

55845898 .. 55848677

Gene Structure 2780 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00374930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1203 bp

ATGGCGGCCACTGGAGCTTTTCCGTCTTCCTACCAAAACCCTAACCTCCAATTTCCCAAACCCTTGTCCTCCTCCTCCGCCGCTTTTGTCATCATCAAAGCACCGACCACAAAGACGAAGAAGCACAGCACCAATAGCACCAGGTGCGAGATCACCAGCACCAACAATTGGACCGTCGAGCCGGACCCTCCGGTTCATTTCTTCAACAAAAACAACTCGCCGGACCACCTTCCGGTGCCAATGGCTTCCGAATCGGCCGTCAAGAAGGTCTGCCTCTTCTACTGCGCCGAGACCAAAGAGCTCGCTCAGAAAGTCGCCGCCGAGTCCGACGGAATTGAGCTCCGTAGCATCACCTGGAGGAAATTTGCTGATGGATTCCCTAACATTTTCATACCTAATGCTCATGCGATTCGCGGACAGCATGTGGCGTTTCTGGCCTCGTTTAATTCTCCGTCGGTGATTTTTGAGCAGCTCTCTGTAATCTATGCATTGCCGAAGCTGTTCATCGCCTCATTCACGCTTGTGCTTCCATTTTTTCCGACTGGGACGTCGGAGCGTATGGAGGATGAGGGAGATGTTGCCACTGCTTTCACACTGGCAAGGGCATTGTCGAATATACCGATTTCGATGGGAGGGCCGACTAGTTTGGTTATCTATGATATCCATGCCTTGCAGGAGAGGTTTTACTTTGGTGATAATATCTTGCCGTGCTTTGAGAGTGGCATCCCATTGCTTAAAACTAGGCTTCAACAGCTCCCTGATTCTGACAATATAACCATTGCTTTTCCTGATGATGGTGCATGGAAACGTTTTCATAAGCAGCTACAGCACTTCCCAACGATTGTTTGTGCCAAAGTTCGGGAAGGTGACCAACGCATTGTTCGTATTAAAGAGGGAGACCCTAAAGGACGGCATGTTGTGATTGTTGATGATTTGGTTCAATCTGGTGGCACATTGATTGAATGTCAGAAAGTTTTAGCCGCCCATGGAGCAGGAAAGATAAGTGCTTATGTAACACATGGGATTTTCCCTAATAGATCATGGGAACGCTTCGGAAGTGATAAAGGAGGGAACCCTGAGCAGGGGCTCACATACTTCTGGATTACAGACTCGTGTCCCACCACAGTGAGAGATGTGATGCACAATCCACCATTTGAAGTTCTTAGCCTTGCTGGTTCCATAGCGACTACTCTTCAAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pribosyltran Pribosyltran_N

Protein Analysis

400

Amino Acids

44.09

Weight (kDa)

6.86

Isoelectric Point (pI)

47.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pribosyltran_N	PF13793	91 - 204	1.2e-08	N-terminal domain of ribose phosphate pyrophosphokinase
Pribosyltran	PF00156	234 - 340	1.1e-09	Phosphoribosyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007309)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10700 AT1G10700
fragaria_vesca	FvH4_4g25920 FvH4_4g25920 FvH4_4g25920 FvH4_4g25920 FvH4_4g25920
malus_domestica	MD13G1098100.v1.1 MD16G1099000.v1.1
prunus_persica	Prupe.1G247200_v2.0.a1 Prupe.1G247200_v2.0.a1
pyrus_communis	pycom13g08570 pycom16g08430
rosa_chinensis	RchiOBHm_Chr4g0433381
rosa_laevigata	RLG00000006758
rosa_multiflora	Rmu_sc0000759.1_g000008
rosa_roxburghii	Rroxscaffold_5G00374930
rosa_rugosa	Rorug04G0268100.1 Rorug04G0268200 Rorug04G0268300
rosa_samantha	Rh4AG323100 Rh4BG330700 Rh4CG346100 Rh4DG327200
rosa_wichuraiana	Rw4G027980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	547
AatII	GACGTC	1 cut(s)	551
AccB1I	GGYRCC	1 cut(s)	235
AccB7I	CCANNNNNTGG	1 cut(s)	168
AccII	CGCG	1 cut(s)	414
AciI	CCGC	6 cut(s)	5, 78, 81, 318, 414, 981
AclI	AACGTT	1 cut(s)	808
AcoI	YGGCCR	2 cut(s)	6, 255
AcsI	RAATTY	1 cut(s)	362
AcyI	GRCGYC	1 cut(s)	548
AdeI	CACNNNGTG	1 cut(s)	144
AfiI	CCNNNNNNNGG	6 cut(s)	168, 232, 794, 858, 1081, 1082
AgsI	TTSAA	6 cut(s)	205, 749, 941, 962, 1157, 1196
AhlI	ACTAGT	1 cut(s)	641
AjnI	CCWGG	2 cut(s)	140, 353
AjuI	GAANNNNNNNTTGG	2 cut(s)	864, 896
AleI	CACNNNNGTG	2 cut(s)	521, 1124
AluBI	AGCT	7 cut(s)	17, 301, 340, 472, 499, 754, 823
AluI	AGCT	7 cut(s)	17, 301, 340, 472, 499, 754, 823
Alw21I	GWGCWC	2 cut(s)	303, 342
Alw26I	GTCTC	2 cut(s)	284, 891
AoxI	GGCC	4 cut(s)	6, 255, 435, 635
ApeKI	GCWGC	2 cut(s)	469, 820
ApoI	RAATTY	1 cut(s)	362
ArsI	GACNNNNNNTTYG	2 cut(s)	243, 275
Asp700I	GAANNNNTTC	1 cut(s)	500
AspLEI	GCGC	1 cut(s)	287
AspS9I	GGNCC	4 cut(s)	171, 184, 223, 635
AsuHPI	GGTGA	5 cut(s)	145, 343, 469, 704, 878
AvaII	GGWCC	3 cut(s)	171, 184, 223
BanI	GGYRCC	1 cut(s)	235
BanII	GRGCYC	3 cut(s)	303, 342, 1089
BauI	CACGAG	1 cut(s)	1111
BbsI	GAAGAC	1 cut(s)	18
Bbv12I	GWGCWC	2 cut(s)	303, 342
BbvI	GCAGC	2 cut(s)	481, 832
BccI	CCATC	3 cut(s)	365, 622, 788
BceAI	ACGGC	3 cut(s)	242, 691, 926
BciT130I	CCWGG	2 cut(s)	142, 355
BcoDI	GTCTC	2 cut(s)	284, 891
BcuI	ACTAGT	1 cut(s)	641
BfaI	CTAG	2 cut(s)	642, 741
BfmI	CTRYAG	1 cut(s)	824
BisI	GCNGC	6 cut(s)	6, 81, 318, 470, 821, 981
BlsI	GCNGC	6 cut(s)	7, 82, 319, 471, 822, 982
Bme1390I	CCNGG	2 cut(s)	142, 355
Bme18I	GGWCC	3 cut(s)	171, 184, 223
BmgT120I	GGNCC	4 cut(s)	171, 184, 223, 635
BmiI	GGNNCC	4 cut(s)	186, 237, 1073, 1177
BmrFI	CCNGG	2 cut(s)	142, 355
BmrI	ACTGGG	1 cut(s)	552
BmsI	GCATC	3 cut(s)	357, 732, 1128
BmuI	ACTGGG	1 cut(s)	552
BoxI	GACNNNNGTC	1 cut(s)	1113
BpiI	GAAGAC	1 cut(s)	18
BplI	GAGNNNNNCTC	2 cut(s)	458, 490
BpmI	CTGGAG	2 cut(s)	33, 376
Bpu10I	CCTNAGC	1 cut(s)	1077
BsaHI	GRCGYC	1 cut(s)	548
BsaI	GGTCTC	2 cut(s)	284, 891
BsaJI	CCNNGG	1 cut(s)	985
BsaWI	WCCGGW	2 cut(s)	190, 232
Bsc4I	CCNNNNNNNGG	6 cut(s)	168, 232, 794, 858, 1081, 1082
Bse1I	ACTGG	3 cut(s)	16, 547, 600
Bse3DI	GCAATG	3 cut(s)	488, 728, 777
BseBI	CCWGG	2 cut(s)	142, 355
BseDI	CCNNGG	1 cut(s)	985
BseGI	GGATG	2 cut(s)	571, 723
BseLI	CCNNNNNNNGG	6 cut(s)	168, 232, 794, 858, 1081, 1082
BseMI	GCAATG	3 cut(s)	488, 728, 777
BseMII	CTCAG	2 cut(s)	320, 1068
BseNI	ACTGG	3 cut(s)	16, 547, 600
BseRI	GAGGAG	2 cut(s)	61, 64
BseX3I	CGGCCG	1 cut(s)	255
BseXI	GCAGC	2 cut(s)	481, 832
Bsh1236I	CGCG	1 cut(s)	414
Bsh1285I	CGRYCG	1 cut(s)	258
BshFI	GGCC	4 cut(s)	8, 257, 437, 637
BshNI	GGYRCC	1 cut(s)	235
BsiEI	CGRYCG	1 cut(s)	258
BsiHKAI	GWGCWC	2 cut(s)	303, 342
BsiSI	CCGG	4 cut(s)	182, 191, 221, 233
BslFI	GGGAC	2 cut(s)	559, 1101
BslI	CCNNNNNNNGG	6 cut(s)	168, 232, 794, 858, 1081, 1082
BsmAI	GTCTC	2 cut(s)	284, 891
BsmFI	GGGAC	2 cut(s)	559, 1101
BsnI	GGCC	4 cut(s)	8, 257, 437, 637
Bso31I	GGTCTC	2 cut(s)	284, 891
Bsp1286I	GDGCHC	3 cut(s)	303, 342, 1089
Bsp143I	GATC	2 cut(s)	150, 1037
Bsp19I	CCATGG	1 cut(s)	985
BspACI	CCGC	6 cut(s)	5, 78, 81, 318, 414, 981
BspANI	GGCC	4 cut(s)	8, 257, 437, 637
BspCNI	CTCAG	2 cut(s)	319, 1069
BspFNI	CGCG	1 cut(s)	414
BspLI	GGNNCC	4 cut(s)	186, 237, 1073, 1177
BspT107I	GGYRCC	1 cut(s)	235
BspTNI	GGTCTC	2 cut(s)	284, 891
BsrDI	GCAATG	3 cut(s)	488, 728, 777
BsrI	ACTGG	3 cut(s)	16, 547, 600
BssECI	CCNNGG	1 cut(s)	985
BssMI	GATC	2 cut(s)	150, 1037
BssNI	GRCGYC	1 cut(s)	548
BssSI	CACGAG	1 cut(s)	1111
BssT1I	CCWWGG	1 cut(s)	985
Bst2BI	CACGAG	1 cut(s)	1111
Bst2UI	CCWGG	2 cut(s)	142, 355
Bst4CI	ACNGT	2 cut(s)	175, 1126
Bst6I	CTCTTC	2 cut(s)	281, 1197
BstACI	GRCGYC	1 cut(s)	548
BstAPI	GCANNNNNTGC	1 cut(s)	144
BstC8I	GCNNGC	1 cut(s)	303
BstDEI	CTNAG	3 cut(s)	306, 1077, 1163
BstDSI	CCRYGG	1 cut(s)	985
BstEII	GGTNACC	1 cut(s)	866
BstF5I	GGATG	2 cut(s)	571, 723
BstFNI	CGCG	1 cut(s)	414
BstHHI	GCGC	1 cut(s)	287
BstKTI	GATC	2 cut(s)	153, 1040
BstMAI	GTCTC	2 cut(s)	284, 891
BstMBI	GATC	2 cut(s)	150, 1037
BstMCI	CGRYCG	1 cut(s)	258
BstMWI	GCNNNNNNNGC	7 cut(s)	14, 135, 144, 434, 751, 826, 989
BstNI	CCWGG	2 cut(s)	142, 355
BstNSI	RCATGY	2 cut(s)	425, 917
BstPAI	GACNNNNGTC	1 cut(s)	1113
BstPI	GGTNACC	1 cut(s)	866
BstSCI	CCNGG	2 cut(s)	140, 353
BstSFI	CTRYAG	1 cut(s)	824
BstUI	CGCG	1 cut(s)	414
BstV1I	GCAGC	2 cut(s)	481, 832
BstV2I	GAAGAC	1 cut(s)	18
BstZI	CGGCCG	1 cut(s)	255
BsuRI	GGCC	4 cut(s)	8, 257, 437, 637
BtgI	CCRYGG	1 cut(s)	985
BtgZI	GCGATG	1 cut(s)	490
BtsCI	GGATG	2 cut(s)	571, 723
BtsI	GCAGTG	1 cut(s)	582
BtsIMutI	CAGTG	4 cut(s)	9, 582, 593, 1131
Cac8I	GCNNGC	1 cut(s)	303
CfoI	GCGC	1 cut(s)	287
Cfr13I	GGNCC	4 cut(s)	171, 184, 223, 635
CsiI	ACCWGGT	1 cut(s)	140
CviAII	CATG	8 cut(s)	404, 422, 665, 801, 914, 986, 1019, 1041
DdeI	CTNAG	3 cut(s)	306, 1077, 1163
DpnI	GATC	2 cut(s)	152, 1039
DpnII	GATC	2 cut(s)	150, 1037
DraIII	CACNNNGTG	1 cut(s)	144
DrdI	GACNNNNNNGTC	1 cut(s)	547
DseDI	GACNNNNNNGTC	1 cut(s)	547
EaeI	YGGCCR	2 cut(s)	6, 255
EagI	CGGCCG	1 cut(s)	255
Eam1104I	CTCTTC	2 cut(s)	281, 1197
EarI	CTCTTC	2 cut(s)	281, 1197
EciI	GGCGGA	1 cut(s)	67
Ecl136II	GAGCTC	2 cut(s)	301, 340
EclXI	CGGCCG	1 cut(s)	255
Eco130I	CCWWGG	1 cut(s)	985
Eco24I	GRGCYC	3 cut(s)	303, 342, 1089
Eco31I	GGTCTC	2 cut(s)	284, 891
Eco32I	GATATC	1 cut(s)	661
Eco47I	GGWCC	3 cut(s)	171, 184, 223
Eco52I	CGGCCG	1 cut(s)	255
Eco53kI	GAGCTC	2 cut(s)	301, 340
Eco91I	GGTNACC	1 cut(s)	866
EcoICRI	GAGCTC	2 cut(s)	301, 340
EcoO65I	GGTNACC	1 cut(s)	866
EcoRII	CCWGG	2 cut(s)	140, 353
EcoRV	GATATC	1 cut(s)	661
EcoT14I	CCWWGG	1 cut(s)	985
EcoT22I	ATGCAT	1 cut(s)	490
EcoT38I	GRGCYC	3 cut(s)	303, 342, 1089
ErhI	CCWWGG	1 cut(s)	985
FaeI	CATG	8 cut(s)	407, 425, 668, 804, 917, 989, 1022, 1044
FaqI	GGGAC	2 cut(s)	559, 1101
FatI	CATG	8 cut(s)	403, 421, 664, 800, 913, 985, 1018, 1040
Fnu4HI	GCNGC	6 cut(s)	6, 81, 318, 470, 821, 981
FokI	GGATG	2 cut(s)	578, 710
FriOI	GRGCYC	3 cut(s)	303, 342, 1089
Fsp4HI	GCNGC	6 cut(s)	6, 81, 318, 470, 821, 981
FspBI	CTAG	2 cut(s)	642, 741
GlaI	GCGC	1 cut(s)	286
GluI	GCNGC	6 cut(s)	6, 81, 318, 470, 821, 981
GsuI	CTGGAG	2 cut(s)	33, 376
HaeIII	GGCC	4 cut(s)	8, 257, 437, 637
HapII	CCGG	4 cut(s)	182, 191, 221, 233
HhaI	GCGC	1 cut(s)	287
Hin1I	GRCGYC	1 cut(s)	548
Hin1II	CATG	8 cut(s)	407, 425, 668, 804, 917, 989, 1022, 1044
Hin6I	GCGC	1 cut(s)	285
HinP1I	GCGC	1 cut(s)	285
HinfI	GANTC	6 cut(s)	251, 323, 375, 409, 761, 1109
HpaII	CCGG	4 cut(s)	182, 191, 221, 233
HphI	GGTGA	5 cut(s)	145, 343, 469, 704, 878
Hpy188I	TCNGA	8 cut(s)	250, 309, 328, 540, 553, 766, 969, 1055
Hpy188III	TCNNGA	4 cut(s)	262, 788, 860, 1099
Hpy99I	CGWCG	4 cut(s)	179, 332, 457, 553
HpyAV	CCTTC	3 cut(s)	239, 259, 857
HpyCH4III	ACNGT	2 cut(s)	175, 1126
HpyCH4IV	ACGT	2 cut(s)	548, 808
HpyCH4V	TGCA	4 cut(s)	488, 673, 800, 1141
HpyF10VI	GCNNNNNNNGC	7 cut(s)	14, 135, 144, 434, 751, 826, 989
HpyF3I	CTNAG	3 cut(s)	306, 1077, 1163
HpySE526I	ACGT	2 cut(s)	548, 808
Hsp92I	GRCGYC	1 cut(s)	548
Hsp92II	CATG	8 cut(s)	407, 425, 668, 804, 917, 989, 1022, 1044
HspAI	GCGC	1 cut(s)	285
Kzo9I	GATC	2 cut(s)	150, 1037
LmnI	GCTCC	5 cut(s)	14, 345, 553, 759, 989
Lsp1109I	GCAGC	2 cut(s)	481, 832
LweI	GCATC	3 cut(s)	357, 732, 1128
MabI	ACCWGGT	1 cut(s)	140
MaeI	CTAG	2 cut(s)	642, 741
MaeII	ACGT	2 cut(s)	548, 808
MaeIII	GTNAC	2 cut(s)	866, 1012
MalI	GATC	2 cut(s)	152, 1039
MboI	GATC	2 cut(s)	150, 1037
MboII	GAAGA	5 cut(s)	18, 130, 193, 268, 1184
MfeI	CAATTG	1 cut(s)	166
MhlI	GDGCHC	3 cut(s)	303, 342, 1089
MluCI	AATT	5 cut(s)	50, 166, 333, 362, 445
MlyI	GAGTC	2 cut(s)	332, 1103
MmeI	TCCRAC	3 cut(s)	351, 531, 563
Mph1103I	ATGCAT	1 cut(s)	490
MroXI	GAANNNNTTC	1 cut(s)	500
MseI	TTAA	3 cut(s)	444, 735, 888
MslI	CAYNNNNRTG	2 cut(s)	521, 1124
MspI	CCGG	4 cut(s)	182, 191, 221, 233
MspR9I	CCNGG	2 cut(s)	142, 355
MunI	CAATTG	1 cut(s)	166
MvaI	CCWGG	2 cut(s)	142, 355
MvnI	CGCG	1 cut(s)	414
MwoI	GCNNNNNNNGC	7 cut(s)	14, 135, 144, 434, 751, 826, 989
NcoI	CCATGG	1 cut(s)	985
NdeII	GATC	2 cut(s)	150, 1037
NlaIII	CATG	8 cut(s)	407, 425, 668, 804, 917, 989, 1022, 1044
NlaIV	GGNNCC	4 cut(s)	186, 237, 1073, 1177
NmeAIII	GCCGAG	2 cut(s)	313, 346
NmuCI	GTSAC	1 cut(s)	866
NsiI	ATGCAT	1 cut(s)	490
NspI	RCATGY	2 cut(s)	425, 917
OliI	CACNNNNGTG	2 cut(s)	521, 1124
PcsI	WCGNNNNNNNCGW	2 cut(s)	617, 880
PdmI	GAANNNNTTC	1 cut(s)	500
PfeI	GAWTC	4 cut(s)	251, 375, 409, 761
PflMI	CCANNNNNTGG	1 cut(s)	168
PkrI	GCNGC	6 cut(s)	7, 82, 319, 471, 822, 982
PleI	GAGTC	2 cut(s)	331, 1103
PpsI	GAGTC	2 cut(s)	331, 1103
PshAI	GACNNNNGTC	1 cut(s)	1113
Psp124BI	GAGCTC	2 cut(s)	303, 342
Psp1406I	AACGTT	1 cut(s)	808
Psp6I	CCWGG	2 cut(s)	140, 353
PspEI	GGTNACC	1 cut(s)	866
PspGI	CCWGG	2 cut(s)	140, 353
PspN4I	GGNNCC	4 cut(s)	186, 237, 1073, 1177
PspPI	GGNCC	4 cut(s)	171, 184, 223, 635
RseI	CAYNNNNRTG	2 cut(s)	521, 1124
SacI	GAGCTC	2 cut(s)	303, 342
SaqAI	TTAA	3 cut(s)	444, 735, 888
SatI	GCNGC	6 cut(s)	6, 81, 318, 470, 821, 981
Sau3AI	GATC	2 cut(s)	150, 1037
Sau96I	GGNCC	4 cut(s)	171, 184, 223, 635
SchI	GAGTC	2 cut(s)	332, 1103
ScrFI	CCNGG	2 cut(s)	142, 355
SduI	GDGCHC	3 cut(s)	303, 342, 1089
SexAI	ACCWGGT	1 cut(s)	140
SfaNI	GCATC	3 cut(s)	357, 732, 1128
SfcI	CTRYAG	1 cut(s)	824
SinI	GGWCC	3 cut(s)	171, 184, 223
SmiMI	CAYNNNNRTG	2 cut(s)	521, 1124
SpeI	ACTAGT	1 cut(s)	641
Sse9I	AATT	5 cut(s)	50, 166, 333, 362, 445
SsiI	CCGC	6 cut(s)	5, 78, 81, 318, 414, 981
SspMI	CTAG	2 cut(s)	642, 741
SstI	GAGCTC	2 cut(s)	303, 342
StyD4I	CCNGG	2 cut(s)	140, 353
StyI	CCWWGG	1 cut(s)	985
TaaI	ACNGT	2 cut(s)	175, 1126
TaiI	ACGT	2 cut(s)	551, 811
TaqI	TCGA	3 cut(s)	177, 611, 626
TasI	AATT	5 cut(s)	50, 166, 333, 362, 445
TauI	GCSGC	4 cut(s)	8, 83, 320, 983
TfiI	GAWTC	4 cut(s)	251, 375, 409, 761
Tru1I	TTAA	3 cut(s)	444, 735, 888
Tru9I	TTAA	3 cut(s)	444, 735, 888
TscAI	CASTG	4 cut(s)	16, 589, 600, 1131
TseFI	GTSAC	1 cut(s)	866
TseI	GCWGC	2 cut(s)	469, 820
Tsp45I	GTSAC	1 cut(s)	866
TspDTI	ATGAA	4 cut(s)	185, 379, 493, 803
TspGWI	ACGGA	4 cut(s)	12, 332, 345, 441
TspRI	CASTG	4 cut(s)	16, 589, 600, 1131
Van91I	CCANNNNNTGG	1 cut(s)	168
VpaK11BI	GGWCC	3 cut(s)	171, 184, 223
XapI	RAATTY	1 cut(s)	362
XceI	RCATGY	2 cut(s)	425, 917
XmnI	GAANNNNTTC	1 cut(s)	500
XspI	CTAG	2 cut(s)	642, 741
ZraI	GACGTC	1 cut(s)	549
Zsp2I	ATGCAT	1 cut(s)	490

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.