pycom16g08430

Ribose-phosphate pyrophosphokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007309
Physical Location & Seq
Reverse (-)
5601388 .. 5603742
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g08430.3

Sequence Viewer

Length: 1209 bp
ATGGCGGCCACTCGACCTCTTCCGCCATCCTTCCAAAACCCTAACTTTCCACCCGCGCCCAAATCTCCCGATCCCTCCTCGGCCGCTTTCCATATCAAAGCCTCCACCTTTAGGAAGAAGAAGCAGAACAGCAGCAACATCATCAGGTGCGAGATCAAGAGCTTCGGCCACAACCTGACCATCGAGCCGGACTCTCCGGTTCACGTCTTCCGCGACACTTCCGAACCCCAATTGTCAATGGCTTCCGATTCGGCGGTGAAGAAGGCGAAGAAGGTCTGCCTCTTCTACTGCGCCGAGACCAAAGCCCTCGCCGAGAATGTCGCCGCCCAGTCCGACTCCATCGAGCTCCGCAGCATTAATTGGAGGAAATTTGATGATGGGTTCCCCAACATTTTCATACCCAATGCTCATGGAATTCGAGGGCAACATGTGGCGTTTCTCGCCTCGTTTAGCTCCCCGTCGGTGATTTTTGAGCAGCTCTCTGTGATTTATGCATTGCCCAAGTTGTTCATCTCCTCATTCACTCTGGTGCTTCCATTTTTTCCGACGGGGACTTCGGAGCGTATGGAGGATGAGGGAGATGTCGCCACCGCTTTCACGCTTGCCAGGGCCTTGTCAAACATTCCCATTTCGATGGGAGGGCCTACTAGTTTAGTTATCTATGATATCCATGCCTTGCAGGAGCGGTTTTACTTTGGTGATAATATCTTACCGTGCTTTGAGAGCGGGATCCCCTTGCTCAAAAATAGGCTCCAGCAGCTCCCTGATTCCGACAATATATCCATTGCTTTTCCTGATGATGGTGCGTGGAAACGATTTCATAAGCAGCTACATCATTTCCCTACGATTGTCTGCGCTAAAGTTCGGGAAGGTGACCAACGAATTGTACGTATTAAGGAAGGAGACCCTAGAGGACGACATGTTGTGATTGTTGATGATTTGGTTCAGTCTGGTGGTACCTTGATCGAATGCCAGAAAGTTTTAGCCGCCCATGGAGCGGCAAAAATCAGTGCTTATGTAACGCATGGGATCTTTCCTAATCGATCATGGGAACGCTTTCAAAGTGATTATGGAGGGCACCCCGAGCACGGGCTGACCTACTTCTGGATTACGGACTCGTGTCCGGTGACAGTGAAAGATGTGAAGAACAATCCGCCATTTGAAGTTCTTAGCCTTGCTGGTTCCATAGCGACTACTCTTCAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pribosyltran Pribosyltran_N

Protein Analysis

403

Amino Acids

44.52

Weight (kDa)

7.68

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 956
AccB1I GGYRCC 2 cut(s) 956, 1077
AccBSI CCGCTC 3 cut(s) 685, 726, 998
AccII CGCG 2 cut(s) 56, 213
AclWI GGATC 4 cut(s) 65, 724, 737, 1037
AcoI YGGCCR 3 cut(s) 6, 81, 166
AcsI RAATTY 2 cut(s) 368, 414
AfaI GTAC 2 cut(s) 888, 958
AfiI CCNNNNNNNGG 6 cut(s) 111, 800, 997, 1088, 1089, 1104
AflIII ACRYGT 2 cut(s) 427, 919
AgsI TTSAA 3 cut(s) 1061, 1163, 1202
AhlI ACTAGT 1 cut(s) 647
AjiI CACGTC 1 cut(s) 205
AjnI CCWGG 1 cut(s) 605
AleI CACNNNNGTG 1 cut(s) 527
AluBI AGCT 6 cut(s) 162, 346, 453, 478, 760, 829
AluI AGCT 6 cut(s) 162, 346, 453, 478, 760, 829
Alw21I GWGCWC 2 cut(s) 348, 1089
Alw26I GTCTC 2 cut(s) 290, 897
AlwI GGATC 4 cut(s) 65, 724, 737, 1037
Ama87I CYCGRG 1 cut(s) 1082
AoxI GGCC 5 cut(s) 6, 81, 166, 609, 641
ApeKI GCWGC 5 cut(s) 132, 351, 475, 757, 826
ApoI RAATTY 2 cut(s) 368, 414
ArsI GACNNNNNNTTYG 2 cut(s) 538, 570
AseI ATTAAT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 1056
Asp718I GGTACC 1 cut(s) 956
AspLEI GCGC 3 cut(s) 58, 293, 857
AspS9I GGNCC 2 cut(s) 609, 641
AsuHPI GGTGA 5 cut(s) 268, 475, 710, 884, 1138
AvaI CYCGRG 1 cut(s) 1082
BaeGI GKGCMC 1 cut(s) 1080
BamHI GGATCC 1 cut(s) 729
BanI GGYRCC 2 cut(s) 956, 1077
BanII GRGCYC 1 cut(s) 348
BauI CACGAG 1 cut(s) 1117
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 2 cut(s) 348, 1089
BbvI GCAGC 5 cut(s) 144, 363, 487, 769, 838
BccI CCATC 6 cut(s) 34, 188, 347, 371, 628, 794
BciT130I CCWGG 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 290, 897
BcuI ACTAGT 1 cut(s) 647
BfaI CTAG 2 cut(s) 648, 909
Bme1390I CCNGG 1 cut(s) 607
BmeT110I CYCGRG 1 cut(s) 1082
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 2 cut(s) 609, 641
BmiI GGNNCC 6 cut(s) 383, 731, 752, 958, 1079, 1183
BmrFI CCNGG 1 cut(s) 607
BmrI ACTGGG 1 cut(s) 322
BmuI ACTGGG 1 cut(s) 322
BoxI GACNNNNGTC 1 cut(s) 1119
BpiI GAAGAC 1 cut(s) 199
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 464, 496
BpmI CTGGAG 1 cut(s) 737
Bsa29I ATCGAT 1 cut(s) 1042
BsaAI YACGTR 1 cut(s) 890
BsaI GGTCTC 2 cut(s) 290, 897
BsaJI CCNNGG 3 cut(s) 78, 606, 991
BsaWI WCCGGW 2 cut(s) 196, 1123
Bsc4I CCNNNNNNNGG 6 cut(s) 111, 800, 997, 1088, 1089, 1104
Bse1I ACTGG 1 cut(s) 328
Bse3DI GCAATG 2 cut(s) 494, 783
BseBI CCWGG 1 cut(s) 607
BseCI ATCGAT 1 cut(s) 1042
BseDI CCNNGG 3 cut(s) 78, 606, 991
BseGI GGATG 2 cut(s) 26, 577
BseLI CCNNNNNNNGG 6 cut(s) 111, 800, 997, 1088, 1089, 1104
BseMI GCAATG 2 cut(s) 494, 783
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 2 cut(s) 67, 505
BseSI GKGCMC 1 cut(s) 1080
BseX3I CGGCCG 1 cut(s) 81
BseXI GCAGC 5 cut(s) 144, 363, 487, 769, 838
Bsh1236I CGCG 2 cut(s) 56, 213
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 5 cut(s) 8, 83, 168, 611, 643
BshNI GGYRCC 2 cut(s) 956, 1077
BshVI ATCGAT 1 cut(s) 1042
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 2 cut(s) 348, 1089
BsiHKCI CYCGRG 1 cut(s) 1082
BsiSI CCGG 3 cut(s) 188, 197, 1124
BslFI GGGAC 1 cut(s) 565
BslI CCNNNNNNNGG 6 cut(s) 111, 800, 997, 1088, 1089, 1104
BsmAI GTCTC 2 cut(s) 290, 897
BsmFI GGGAC 1 cut(s) 565
BsmI GAATGC 1 cut(s) 974
BsnI GGCC 5 cut(s) 8, 83, 168, 611, 643
Bso31I GGTCTC 2 cut(s) 290, 897
BsoBI CYCGRG 1 cut(s) 1082
Bsp1286I GDGCHC 3 cut(s) 348, 1080, 1089
Bsp143I GATC 6 cut(s) 70, 153, 729, 963, 1029, 1043
Bsp19I CCATGG 1 cut(s) 991
BspANI GGCC 5 cut(s) 8, 83, 168, 611, 643
BspDI ATCGAT 1 cut(s) 1042
BspFNI CGCG 2 cut(s) 56, 213
BspLI GGNNCC 6 cut(s) 383, 731, 752, 958, 1079, 1183
BspPI GGATC 4 cut(s) 65, 724, 737, 1037
BspT107I GGYRCC 2 cut(s) 956, 1077
BspTNI GGTCTC 2 cut(s) 290, 897
BsrBI CCGCTC 3 cut(s) 685, 726, 998
BsrDI GCAATG 2 cut(s) 494, 783
BsrI ACTGG 1 cut(s) 328
BssECI CCNNGG 3 cut(s) 78, 606, 991
BssMI GATC 6 cut(s) 70, 153, 729, 963, 1029, 1043
BssSI CACGAG 1 cut(s) 1117
BssT1I CCWWGG 1 cut(s) 991
Bst2BI CACGAG 1 cut(s) 1117
Bst2UI CCWGG 1 cut(s) 607
Bst4CI ACNGT 2 cut(s) 714, 1132
Bst6I CTCTTC 3 cut(s) 24, 287, 1203
BstBAI YACGTR 1 cut(s) 890
BstC8I GCNNGC 1 cut(s) 603
BstDEI CTNAG 1 cut(s) 1169
BstDSI CCRYGG 1 cut(s) 991
BstEII GGTNACC 1 cut(s) 872
BstF5I GGATG 2 cut(s) 26, 577
BstFNI CGCG 2 cut(s) 56, 213
BstHHI GCGC 3 cut(s) 58, 293, 857
BstKTI GATC 6 cut(s) 73, 156, 732, 966, 1032, 1046
BstMAI GTCTC 2 cut(s) 290, 897
BstMBI GATC 6 cut(s) 70, 153, 729, 963, 1029, 1043
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 5 cut(s) 440, 723, 757, 995, 1084
BstNI CCWGG 1 cut(s) 607
BstNSI RCATGY 2 cut(s) 431, 923
BstPAI GACNNNNGTC 1 cut(s) 1119
BstPI GGTNACC 1 cut(s) 872
BstSCI CCNGG 1 cut(s) 605
BstSLI GKGCMC 1 cut(s) 1080
BstSNI TACGTA 1 cut(s) 890
BstUI CGCG 2 cut(s) 56, 213
BstV1I GCAGC 5 cut(s) 144, 363, 487, 769, 838
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 2 cut(s) 729, 1029
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 2 cut(s) 729, 1029
BstZI CGGCCG 1 cut(s) 81
Bsu15I ATCGAT 1 cut(s) 1042
BsuRI GGCC 5 cut(s) 8, 83, 168, 611, 643
BsuTUI ATCGAT 1 cut(s) 1042
BtgI CCRYGG 1 cut(s) 991
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 2 cut(s) 26, 577
BtsIMutI CAGTG 2 cut(s) 1015, 1137
Cac8I GCNNGC 1 cut(s) 603
CfoI GCGC 3 cut(s) 58, 293, 857
Cfr13I GGNCC 2 cut(s) 609, 641
ClaI ATCGAT 1 cut(s) 1042
Csp6I GTAC 2 cut(s) 887, 957
CviAII CATG 7 cut(s) 410, 428, 671, 920, 992, 1025, 1047
CviQI GTAC 2 cut(s) 887, 957
DdeI CTNAG 1 cut(s) 1169
DpnI GATC 6 cut(s) 72, 155, 731, 965, 1031, 1045
DpnII GATC 6 cut(s) 70, 153, 729, 963, 1029, 1043
EaeI YGGCCR 3 cut(s) 6, 81, 166
EagI CGGCCG 1 cut(s) 81
Eam1104I CTCTTC 3 cut(s) 24, 287, 1203
EarI CTCTTC 3 cut(s) 24, 287, 1203
EciI GGCGGA 2 cut(s) 12, 1143
Ecl136II GAGCTC 1 cut(s) 346
EclXI CGGCCG 1 cut(s) 81
Eco105I TACGTA 1 cut(s) 890
Eco130I CCWWGG 1 cut(s) 991
Eco24I GRGCYC 1 cut(s) 348
Eco31I GGTCTC 2 cut(s) 290, 897
Eco32I GATATC 1 cut(s) 667
Eco52I CGGCCG 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 346
Eco88I CYCGRG 1 cut(s) 1082
Eco91I GGTNACC 1 cut(s) 872
EcoICRI GAGCTC 1 cut(s) 346
EcoO109I RGGNCCY 2 cut(s) 609, 641
EcoO65I GGTNACC 1 cut(s) 872
EcoRI GAATTC 1 cut(s) 414
EcoRII CCWGG 1 cut(s) 605
EcoRV GATATC 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 991
EcoT22I ATGCAT 1 cut(s) 496
EcoT38I GRGCYC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 991
FaeI CATG 7 cut(s) 413, 431, 674, 923, 995, 1028, 1050
FaqI GGGAC 1 cut(s) 565
FatI CATG 7 cut(s) 409, 427, 670, 919, 991, 1024, 1046
FauI CCCGC 2 cut(s) 61, 719
FokI GGATG 2 cut(s) 13, 584
FriOI GRGCYC 1 cut(s) 348
FspBI CTAG 2 cut(s) 648, 909
GlaI GCGC 3 cut(s) 57, 292, 856
GsuI CTGGAG 1 cut(s) 737
HaeIII GGCC 5 cut(s) 8, 83, 168, 611, 643
HapII CCGG 3 cut(s) 188, 197, 1124
HhaI GCGC 3 cut(s) 58, 293, 857
Hin1II CATG 7 cut(s) 413, 431, 674, 923, 995, 1028, 1050
Hin6I GCGC 3 cut(s) 56, 291, 855
HinP1I GCGC 3 cut(s) 56, 291, 855
HinfI GANTC 5 cut(s) 191, 248, 335, 767, 1115
HpaII CCGG 3 cut(s) 188, 197, 1124
HphI GGTGA 5 cut(s) 268, 475, 710, 884, 1138
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 6 cut(s) 223, 247, 334, 546, 559, 772
Hpy188III TCNNGA 5 cut(s) 68, 157, 794, 866, 1105
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 2 cut(s) 463, 550
HpyAV CCTTC 5 cut(s) 40, 256, 265, 863, 893
HpyCH4III ACNGT 2 cut(s) 714, 1132
HpyCH4IV ACGT 2 cut(s) 204, 889
HpyCH4V TGCA 2 cut(s) 494, 679
HpyF10VI GCNNNNNNNGC 5 cut(s) 440, 723, 757, 995, 1084
HpyF3I CTNAG 1 cut(s) 1169
HpySE526I ACGT 2 cut(s) 204, 889
Hsp92II CATG 7 cut(s) 413, 431, 674, 923, 995, 1028, 1050
HspAI GCGC 3 cut(s) 56, 291, 855
KpnI GGTACC 1 cut(s) 960
Kzo9I GATC 6 cut(s) 70, 153, 729, 963, 1029, 1043
LmnI GCTCC 7 cut(s) 351, 458, 559, 682, 756, 765, 995
Lsp1109I GCAGC 5 cut(s) 144, 363, 487, 769, 838
MaeI CTAG 2 cut(s) 648, 909
MaeII ACGT 2 cut(s) 204, 889
MaeIII GTNAC 3 cut(s) 872, 1018, 1126
MalI GATC 6 cut(s) 72, 155, 731, 965, 1031, 1045
MbiI CCGCTC 3 cut(s) 685, 726, 998
MboI GATC 6 cut(s) 70, 153, 729, 963, 1029, 1043
MboII GAAGA 9 cut(s) 11, 127, 130, 199, 271, 274, 280, 1156, 1190
MfeI CAATTG 1 cut(s) 230
MflI RGATCY 2 cut(s) 729, 1029
MhlI GDGCHC 3 cut(s) 348, 1080, 1089
MluCI AATT 5 cut(s) 230, 358, 368, 414, 882
MlyI GAGTC 3 cut(s) 185, 329, 1109
MmeI TCCRAC 3 cut(s) 357, 569, 795
Mph1103I ATGCAT 1 cut(s) 496
MroXI GAANNNNTTC 1 cut(s) 1056
MseI TTAA 2 cut(s) 357, 894
MslI CAYNNNNRTG 2 cut(s) 527, 632
MspI CCGG 3 cut(s) 188, 197, 1124
MspR9I CCNGG 1 cut(s) 607
MunI CAATTG 1 cut(s) 230
Mva1269I GAATGC 1 cut(s) 974
MvaI CCWGG 1 cut(s) 607
MvnI CGCG 2 cut(s) 56, 213
MwoI GCNNNNNNNGC 5 cut(s) 440, 723, 757, 995, 1084
NcoI CCATGG 1 cut(s) 991
NdeII GATC 6 cut(s) 70, 153, 729, 963, 1029, 1043
NlaIII CATG 7 cut(s) 413, 431, 674, 923, 995, 1028, 1050
NlaIV GGNNCC 6 cut(s) 383, 731, 752, 958, 1079, 1183
NmeAIII GCCGAG 3 cut(s) 59, 319, 337
NmuCI GTSAC 2 cut(s) 872, 1126
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 2 cut(s) 431, 923
OliI CACNNNNGTG 1 cut(s) 527
PciI ACATGT 2 cut(s) 427, 919
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 886
PctI GAATGC 1 cut(s) 974
PdmI GAANNNNTTC 1 cut(s) 1056
PfeI GAWTC 2 cut(s) 248, 767
PleI GAGTC 3 cut(s) 185, 329, 1109
PpsI GAGTC 3 cut(s) 185, 329, 1109
Ppu21I YACGTR 1 cut(s) 890
PscI ACATGT 2 cut(s) 427, 919
PshAI GACNNNNGTC 1 cut(s) 1119
PshBI ATTAAT 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 605
PspEI GGTNACC 1 cut(s) 872
PspGI CCWGG 1 cut(s) 605
PspN4I GGNNCC 6 cut(s) 383, 731, 752, 958, 1079, 1183
PspPI GGNCC 2 cut(s) 609, 641
PsuI RGATCY 2 cut(s) 729, 1029
RsaI GTAC 2 cut(s) 888, 958
RsaNI GTAC 2 cut(s) 887, 957
RseI CAYNNNNRTG 2 cut(s) 527, 632
SacI GAGCTC 1 cut(s) 348
SaqAI TTAA 2 cut(s) 357, 894
Sau3AI GATC 6 cut(s) 70, 153, 729, 963, 1029, 1043
Sau96I GGNCC 2 cut(s) 609, 641
SchI GAGTC 3 cut(s) 185, 329, 1109
ScrFI CCNGG 1 cut(s) 607
SduI GDGCHC 3 cut(s) 348, 1080, 1089
SmiMI CAYNNNNRTG 2 cut(s) 527, 632
SnaBI TACGTA 1 cut(s) 890
SpeI ACTAGT 1 cut(s) 647
Sse9I AATT 5 cut(s) 230, 358, 368, 414, 882
SspMI CTAG 2 cut(s) 648, 909
SstI GAGCTC 1 cut(s) 348
StyD4I CCNGG 1 cut(s) 605
StyI CCWWGG 1 cut(s) 991
TaaI ACNGT 2 cut(s) 714, 1132
TaiI ACGT 2 cut(s) 207, 892
TaqI TCGA 7 cut(s) 13, 183, 342, 418, 632, 966, 1042
TasI AATT 5 cut(s) 230, 358, 368, 414, 882
TauI GCSGC 5 cut(s) 8, 86, 326, 989, 1001
TfiI GAWTC 2 cut(s) 248, 767
Tru1I TTAA 2 cut(s) 357, 894
Tru9I TTAA 2 cut(s) 357, 894
TscAI CASTG 2 cut(s) 1015, 1137
TseFI GTSAC 2 cut(s) 872, 1126
TseI GCWGC 5 cut(s) 132, 351, 475, 757, 826
Tsp45I GTSAC 2 cut(s) 872, 1126
TspDTI ATGAA 3 cut(s) 385, 499, 809
TspGWI ACGGA 1 cut(s) 1127
TspRI CASTG 2 cut(s) 1015, 1137
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 2 cut(s) 368, 414
XceI RCATGY 2 cut(s) 431, 923
XmnI GAANNNNTTC 1 cut(s) 1056
XspI CTAG 2 cut(s) 648, 909
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.