pycom13g08720

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001774
Physical Location & Seq
Forward (+)
5771259 .. 5774023
2765 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1095 bp
ATGTATAGTCAGAAGCTTGTAAATAACATGAGAATCCTTTCGCGCAGTTCGGGTGGTAAAAAACCAAATGAGAACATGAGGCTTATTGTGACAACTTTTGTAGGTGTAGCTTTTGGATTCTTGATAGGTGTGTCCTTTCCAACATTGTCACTAACAAAGCTGAATCTCTCATCCAGCCTTCTTCCAACTGTTGATCTTTCATACACTGAGGACAAGAAATCAGACATCTCCAGCCAATCACAAAGTTCTTGGCCTTCTGAAAGGAGTAAAAATAGCTCAAGTCAAGATCAGAGTTCGAATGGCAAATCGAGGATTTGGGTGCCATCAAATCCTAGAGGTGCAGAAATGCTACCACCAGGAATTGTTGCAGCCGAGTCAGACTTCTACTTGCGCAGATTATGGGGCAAGCCTAGTGAGGACTTGACCATCAAACCAAGGTACCTAGTGACATTCACTGTGGGTTTGAATCAGAAAAAGAATATTGATGCAGCAGTGAAAAAGTTTTCAGAGAACTTTACAATTCTCCTTTTTCATTACGATGGCCAAACAACTGAGTGGGATGACTTAGAGTGGTCTAAGCGTGCTATACATATTAGTGCTCGTAAGCAGACTAAATGGTGGTATGCCAAGCGGTTTTTGCATCCTGACATTGTGGCGCCATACGACTACATATTTATCTGGGATGAAGACCTGGGAGTAGAGCATTTTAATGCAGAAGTATACCTAAGACTAGTGAAGAAGCATGGTTTGGAGATTTCACAGCCTGGTTTGGAACCCAACAAAGGTTTAACATGGCAGATGACAAAGAGGAGGGTCGATCGTGAAGTTCATAAACAAACAGAGGAAAAACCGGGCTGGTGCACTGACCCACTTCTGCCTCCATGTGCAGCCTTTATTGAAATCATGGCTCCAGTATTTTCTCGAAGTGCCTGGCGCTGTGTGTGGCATATGATTCAGAATGACTTGGTCCACGGCTGGGGTCTTGATTTTGCCCTGAGAAAATGTGTAGAGCCTGCGCACGAGAAGATTGGCGTTGTAGATTCTCAGTGGATCTGGGGTTCATCAAACTGTTCCTTCATTGGGGAGCCAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

365

Amino Acids

41.6

Weight (kDa)

8.8

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 109 - 355 4e-128 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 392, 1017
Acc65I GGTACC 1 cut(s) 438
AccB1I GGYRCC 3 cut(s) 319, 438, 655
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 43
AciI CCGC 1 cut(s) 631
AclWI GGATC 1 cut(s) 1058
AcoI YGGCCR 1 cut(s) 541
AcyI GRCGYC 1 cut(s) 656
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 3 cut(s) 782, 976, 1080
AgsI TTSAA 2 cut(s) 466, 899
AhlI ACTAGT 1 cut(s) 730
AjnI CCWGG 5 cut(s) 355, 690, 763, 929, 1087
AjuI GAANNNNNNNTTGG 2 cut(s) 731, 763
AloI GAACNNNNNNTCC 2 cut(s) 1042, 1074
AluBI AGCT 4 cut(s) 16, 110, 160, 276
AluI AGCT 4 cut(s) 16, 110, 160, 276
Alw21I GWGCWC 2 cut(s) 601, 863
Alw44I GTGCAC 1 cut(s) 859
AlwI GGATC 1 cut(s) 1058
AoxI GGCC 2 cut(s) 251, 541
ApaLI GTGCAC 1 cut(s) 859
ApeKI GCWGC 3 cut(s) 368, 488, 887
Asp700I GAANNNNTTC 1 cut(s) 37
Asp718I GGTACC 1 cut(s) 438
AspLEI GCGC 5 cut(s) 45, 393, 658, 936, 1018
AspS9I GGNCC 1 cut(s) 967
AsuC2I CCSGG 1 cut(s) 852
AsuII TTCGAA 1 cut(s) 296
AvaII GGWCC 1 cut(s) 967
BaeGI GKGCMC 1 cut(s) 863
BaeI ACNNNNGTAYC 2 cut(s) 422, 455
BalI TGGCCA 1 cut(s) 543
BanI GGYRCC 3 cut(s) 319, 438, 655
BauI CACGAG 1 cut(s) 1019
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 2 cut(s) 601, 863
BbvI GCAGC 3 cut(s) 380, 500, 899
BccI CCATC 3 cut(s) 331, 434, 533
BceAI ACGGC 1 cut(s) 988
BciT130I CCWGG 5 cut(s) 357, 692, 765, 931, 1089
BcnI CCSGG 1 cut(s) 852
BcuI ACTAGT 1 cut(s) 730
BfaI CTAG 4 cut(s) 333, 411, 443, 731
BfoI RGCGCY 2 cut(s) 659, 937
BisI GCNGC 3 cut(s) 369, 489, 888
BlsI GCNGC 3 cut(s) 370, 490, 889
Bme1390I CCNGG 6 cut(s) 357, 692, 765, 852, 931, 1089
Bme18I GGWCC 1 cut(s) 967
BmgT120I GGNCC 1 cut(s) 967
BmiI GGNNCC 6 cut(s) 321, 440, 657, 774, 909, 1086
BmrFI CCNGG 6 cut(s) 357, 692, 765, 852, 931, 1089
BmsI GCATC 2 cut(s) 475, 649
BpiI GAAGAC 1 cut(s) 693
BpmI CTGGAG 2 cut(s) 214, 894
Bpu14I TTCGAA 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 262
BpuMI CCSGG 1 cut(s) 852
BsaHI GRCGYC 1 cut(s) 656
BsaJI CCNNGG 4 cut(s) 434, 691, 970, 1088
BsaXI ACNNNNNCTCC 1 cut(s) 1076
Bsc4I CCNNNNNNNGG 3 cut(s) 782, 976, 1080
Bse1I ACTGG 1 cut(s) 911
BseBI CCWGG 5 cut(s) 357, 692, 765, 931, 1089
BseDI CCNNGG 4 cut(s) 434, 691, 970, 1088
BseGI GGATG 4 cut(s) 170, 565, 640, 688
BseLI CCNNNNNNNGG 3 cut(s) 782, 976, 1080
BseMII CTCAG 4 cut(s) 198, 543, 986, 1058
BseNI ACTGG 1 cut(s) 911
BseRI GAGGAG 1 cut(s) 823
BseSI GKGCMC 1 cut(s) 863
BseXI GCAGC 3 cut(s) 380, 500, 899
BseYI CCCAGC 1 cut(s) 975
BsgI GTGCAG 2 cut(s) 360, 906
Bsh1236I CGCG 1 cut(s) 43
Bsh1285I CGRYCG 1 cut(s) 820
BshFI GGCC 2 cut(s) 253, 543
BshNI GGYRCC 3 cut(s) 319, 438, 655
BsiEI CGRYCG 1 cut(s) 820
BsiHKAI GWGCWC 2 cut(s) 601, 863
BsiSI CCGG 1 cut(s) 851
BslI CCNNNNNNNGG 3 cut(s) 782, 976, 1080
BsnI GGCC 2 cut(s) 253, 543
Bsp119I TTCGAA 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 601, 863
Bsp143I GATC 4 cut(s) 193, 286, 817, 1050
BspACI CCGC 1 cut(s) 631
BspANI GGCC 2 cut(s) 253, 543
BspCNI CTCAG 4 cut(s) 199, 544, 987, 1057
BspFNI CGCG 1 cut(s) 43
BspLI GGNNCC 6 cut(s) 321, 440, 657, 774, 909, 1086
BspPI GGATC 1 cut(s) 1058
BspT104I TTCGAA 1 cut(s) 296
BspT107I GGYRCC 3 cut(s) 319, 438, 655
BsrI ACTGG 1 cut(s) 911
BssECI CCNNGG 4 cut(s) 434, 691, 970, 1088
BssMI GATC 4 cut(s) 193, 286, 817, 1050
BssNAI GTATAC 1 cut(s) 721
BssNI GRCGYC 1 cut(s) 656
BssSI CACGAG 1 cut(s) 1019
BssT1I CCWWGG 1 cut(s) 434
Bst1107I GTATAC 1 cut(s) 721
Bst2BI CACGAG 1 cut(s) 1019
Bst2UI CCWGG 5 cut(s) 357, 692, 765, 931, 1089
Bst4CI ACNGT 3 cut(s) 190, 457, 1070
BstACI GRCGYC 1 cut(s) 656
BstBI TTCGAA 1 cut(s) 296
BstC8I GCNNGC 3 cut(s) 407, 582, 1014
BstDEI CTNAG 7 cut(s) 207, 552, 565, 576, 725, 995, 1044
BstDSI CCRYGG 1 cut(s) 970
BstF5I GGATG 4 cut(s) 170, 565, 640, 688
BstFNI CGCG 1 cut(s) 43
BstH2I RGCGCY 2 cut(s) 659, 937
BstHHI GCGC 5 cut(s) 45, 393, 658, 936, 1018
BstKTI GATC 4 cut(s) 196, 289, 820, 1053
BstMBI GATC 4 cut(s) 193, 286, 817, 1050
BstMCI CGRYCG 1 cut(s) 820
BstMWI GCNNNNNNNGC 1 cut(s) 637
BstNI CCWGG 5 cut(s) 357, 692, 765, 931, 1089
BstSCI CCNGG 6 cut(s) 355, 690, 763, 850, 929, 1087
BstSLI GKGCMC 1 cut(s) 863
BstUI CGCG 1 cut(s) 43
BstV1I GCAGC 3 cut(s) 380, 500, 899
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 1 cut(s) 1050
BstYI RGATCY 1 cut(s) 1050
BstZ17I GTATAC 1 cut(s) 721
BsuRI GGCC 2 cut(s) 253, 543
BtgI CCRYGG 1 cut(s) 970
BtsCI GGATG 4 cut(s) 170, 565, 640, 688
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 5 cut(s) 204, 453, 498, 861, 1052
Cac8I GCNNGC 3 cut(s) 407, 582, 1014
CfoI GCGC 5 cut(s) 45, 393, 658, 936, 1018
Cfr13I GGNCC 1 cut(s) 967
Csp6I GTAC 1 cut(s) 439
CviAII CATG 6 cut(s) 28, 76, 743, 792, 882, 904
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 7 cut(s) 207, 552, 565, 576, 725, 995, 1044
DinI GGCGCC 1 cut(s) 657
DpnI GATC 4 cut(s) 195, 288, 819, 1052
DpnII GATC 4 cut(s) 193, 286, 817, 1050
EaeI YGGCCR 1 cut(s) 541
Eco130I CCWWGG 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 967
EcoRII CCWGG 5 cut(s) 355, 690, 763, 929, 1087
EcoT14I CCWWGG 1 cut(s) 434
EgeI GGCGCC 1 cut(s) 657
EheI GGCGCC 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 434
FaeI CATG 6 cut(s) 31, 79, 746, 795, 885, 907
FatI CATG 6 cut(s) 27, 75, 742, 791, 881, 903
FauNDI CATATG 1 cut(s) 948
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 3 cut(s) 369, 489, 888
FokI GGATG 4 cut(s) 157, 572, 627, 695
Fsp4HI GCNGC 3 cut(s) 369, 489, 888
FspBI CTAG 4 cut(s) 333, 411, 443, 731
FspI TGCGCA 2 cut(s) 392, 1017
GlaI GCGC 5 cut(s) 44, 392, 657, 935, 1017
GluI GCNGC 3 cut(s) 369, 489, 888
GsaI CCCAGC 1 cut(s) 979
GsuI CTGGAG 2 cut(s) 214, 894
HaeII RGCGCY 2 cut(s) 659, 937
HaeIII GGCC 2 cut(s) 253, 543
HapII CCGG 1 cut(s) 851
HhaI GCGC 5 cut(s) 45, 393, 658, 936, 1018
Hin1I GRCGYC 1 cut(s) 656
Hin1II CATG 6 cut(s) 31, 79, 746, 795, 885, 907
Hin6I GCGC 5 cut(s) 43, 391, 656, 934, 1016
HinP1I GCGC 5 cut(s) 43, 391, 656, 934, 1016
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 7 cut(s) 33, 117, 163, 374, 466, 952, 1040
HpaII CCGG 1 cut(s) 851
Hpy166II GTNNAC 3 cut(s) 721, 861, 970
Hpy188I TCNGA 8 cut(s) 12, 223, 259, 291, 379, 471, 508, 957
Hpy188III TCNNGA 6 cut(s) 121, 284, 644, 821, 921, 983
Hpy8I GTNNAC 3 cut(s) 721, 861, 970
HpyAV CCTTC 3 cut(s) 188, 264, 1084
HpyCH4III ACNGT 3 cut(s) 190, 457, 1070
HpyCH4V TGCA 7 cut(s) 341, 368, 488, 640, 713, 861, 887
HpyF10VI GCNNNNNNNGC 1 cut(s) 637
HpyF3I CTNAG 7 cut(s) 207, 552, 565, 576, 725, 995, 1044
Hsp92I GRCGYC 1 cut(s) 656
Hsp92II CATG 6 cut(s) 31, 79, 746, 795, 885, 907
HspAI GCGC 5 cut(s) 43, 391, 656, 934, 1016
KasI GGCGCC 1 cut(s) 655
KpnI GGTACC 1 cut(s) 442
Kzo9I GATC 4 cut(s) 193, 286, 817, 1050
LmnI GCTCC 2 cut(s) 913, 1084
Lsp1109I GCAGC 3 cut(s) 380, 500, 899
LweI GCATC 2 cut(s) 475, 649
MaeI CTAG 4 cut(s) 333, 411, 443, 731
MaeIII GTNAC 3 cut(s) 88, 147, 445
MalI GATC 4 cut(s) 195, 288, 819, 1052
MboI GATC 4 cut(s) 193, 286, 817, 1050
MboII GAAGA 4 cut(s) 173, 698, 748, 1036
MflI RGATCY 1 cut(s) 1050
MhlI GDGCHC 2 cut(s) 601, 863
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 2 cut(s) 360, 519
MluNI TGGCCA 1 cut(s) 543
Mly113I GGCGCC 1 cut(s) 656
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 2 cut(s) 164, 209
MnlI CCTC 9 cut(s) 72, 202, 303, 329, 409, 801, 804, 835, 888
Mox20I TGGCCA 1 cut(s) 543
MroXI GAANNNNTTC 1 cut(s) 37
MscI TGGCCA 1 cut(s) 543
MseI TTAA 2 cut(s) 708, 788
MslI CAYNNNNRTG 3 cut(s) 537, 594, 708
Msp20I TGGCCA 1 cut(s) 543
MspI CCGG 1 cut(s) 851
MspR9I CCNGG 6 cut(s) 357, 692, 765, 852, 931, 1089
MvaI CCWGG 5 cut(s) 357, 692, 765, 931, 1089
MvnI CGCG 1 cut(s) 43
MwoI GCNNNNNNNGC 1 cut(s) 637
NarI GGCGCC 1 cut(s) 656
NciI CCSGG 1 cut(s) 852
NdeI CATATG 1 cut(s) 948
NdeII GATC 4 cut(s) 193, 286, 817, 1050
NlaIII CATG 6 cut(s) 31, 79, 746, 795, 885, 907
NlaIV GGNNCC 6 cut(s) 321, 440, 657, 774, 909, 1086
NmeAIII GCCGAG 1 cut(s) 397
NmuCI GTSAC 3 cut(s) 88, 147, 445
NsbI TGCGCA 2 cut(s) 392, 1017
NspV TTCGAA 1 cut(s) 296
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 6 cut(s) 33, 117, 163, 466, 952, 1040
PflFI GACNNNGTC 1 cut(s) 965
PkrI GCNGC 3 cut(s) 370, 490, 889
Ple19I CGATCG 1 cut(s) 820
PleI GAGTC 1 cut(s) 382
PluTI GGCGCC 1 cut(s) 659
PpsI GAGTC 1 cut(s) 382
Psp6I CCWGG 5 cut(s) 355, 690, 763, 929, 1087
PspFI CCCAGC 1 cut(s) 975
PspGI CCWGG 5 cut(s) 355, 690, 763, 929, 1087
PspN4I GGNNCC 6 cut(s) 321, 440, 657, 774, 909, 1086
PspPI GGNCC 1 cut(s) 967
PsuI RGATCY 1 cut(s) 1050
PsyI GACNNNGTC 1 cut(s) 965
PvuI CGATCG 1 cut(s) 820
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
RseI CAYNNNNRTG 3 cut(s) 537, 594, 708
SaqAI TTAA 2 cut(s) 708, 788
SatI GCNGC 3 cut(s) 369, 489, 888
Sau3AI GATC 4 cut(s) 193, 286, 817, 1050
Sau96I GGNCC 1 cut(s) 967
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 6 cut(s) 357, 692, 765, 852, 931, 1089
SduI GDGCHC 2 cut(s) 601, 863
SfaNI GCATC 2 cut(s) 475, 649
SfoI GGCGCC 1 cut(s) 657
SfuI TTCGAA 1 cut(s) 296
SinI GGWCC 1 cut(s) 967
SmiMI CAYNNNNRTG 3 cut(s) 537, 594, 708
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
SpeI ACTAGT 1 cut(s) 730
Sse9I AATT 2 cut(s) 360, 519
SsiI CCGC 1 cut(s) 631
SspDI GGCGCC 1 cut(s) 655
SspI AATATT 1 cut(s) 481
SspMI CTAG 4 cut(s) 333, 411, 443, 731
StyD4I CCNGG 6 cut(s) 355, 690, 763, 850, 929, 1087
StyI CCWWGG 1 cut(s) 434
TaaI ACNGT 3 cut(s) 190, 457, 1070
TaqI TCGA 4 cut(s) 296, 308, 816, 922
TasI AATT 2 cut(s) 360, 519
TfiI GAWTC 6 cut(s) 33, 117, 163, 466, 952, 1040
Tru1I TTAA 2 cut(s) 708, 788
Tru9I TTAA 2 cut(s) 708, 788
TscAI CASTG 5 cut(s) 211, 460, 498, 868, 1052
TseFI GTSAC 3 cut(s) 88, 147, 445
TseI GCWGC 3 cut(s) 368, 488, 887
Tsp45I GTSAC 3 cut(s) 88, 147, 445
TspDTI ATGAA 6 cut(s) 189, 521, 699, 818, 1050, 1066
TspRI CASTG 5 cut(s) 211, 460, 498, 868, 1052
Tth111I GACNNNGTC 1 cut(s) 965
VneI GTGCAC 1 cut(s) 859
VpaK11BI GGWCC 1 cut(s) 967
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 4 cut(s) 333, 411, 443, 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.