pycom13g17260

Chlorophyllase-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011927
Physical Location & Seq
Forward (+)
13020942 .. 13025496
4555 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1176 bp
ATGCATGAGCGTTTAAAGAGGCTTAAAAGGACTTTAGTTGATCCCATAGGCAGCCTTGTCCGAGTGCATCCATGTATGCAATGTACAAGAGTCCAACTTCAGTGTCAAAATATCTGGGAACTGAACCCCTTATCTGCGCACAAAAAGTTAGATGCTCTCTCAATGACAGCCATGTCCTCCTCCACTTCTTCTTCTTCGTTTGCCATCTCTGGTACAAATGTTTTTGAGATGGGAAAATACAAAGTTGTCTTCCAGAGAGTTGAAGCAGCAGAGAGGAGTGGATGCAGCAGCAAAACCAAGTCGTCGTCGTCGTCGTCAACTTCTCCTCCTGCTAACCCACCGCCTCCGAAACCACTATTGATTGGCATGCCTTGTGAAGCTGCTGGAGAGTTCCCAGTGTTGCTGCTCCTCCATGGTTATCTTCTCTCCAACTCTTTCTACTCTCAGCTTATCAACCACATCGCTTCTCATGGCTTCATTGTCATTGCCCCTCAGTTATATACCATAGCTGGACCAGACACAACTGATGAGATCAAGTCCACGGCTGCAATAACAAACTGGTTATCTAATGGACTTCAACCCTTGCTTCCACCCAATGTTCATGCAAATTTAAGCAAGCTAGCACTCGCCGGCCATAGCCGCGGAGGCAAGGTTTCATTTGCACTAGCTTTAGGGAAAGAAGAAACTGGCACCAGCTTAAAGTTTTCAGCCTTGATAGGCATTGACCCAGTTGACGGAATGGACAAGGGGAAACAAACTCCTCCGCCAGTGCTAACCTATGTTCCTCATTCATTTGATCTTGACATGGCAGTGATGGTGATTGGTTCGGGTTTGGGTGAAGTGAAAAAGAACCCTCTATTCCCTCCTTGTGCTCCAAAGGGTGTTAACCATGAGGACTTCTTCAACGAATGCCGGAAATTGGCTTGTTATTTTGTGGCAAAGGATTATGGCCATCTTGACATGCTAGATGATGAGACCAAAGGGATTAGAGGGAAAGCTACGTATTGTTTGTGCAAAAATGGGAAGTCTAGGGAGCCCATGAGGAGGTTTGTTGGAGGGGTTGTGGTTGCTTTCATAAAAGCTTATTTAGAAGGTGATAGTAGCCACCTATTGGCTATAAGAGAAGGGGTTGAGACTTTGCCCATAGAGCTTCAATGTGTTAAATTTCTTTTGTGA

Protein Analysis

392

Amino Acids

42.41

Weight (kDa)

8.77

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chlorophyllase PF07224 72 - 389 3.9e-136 Chlorophyllase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43860
fragaria_vesca FvH4_4g00030 FvH4_4g01061 FvH4_4g01061
malus_domestica MD13G1198800.v1.1 MD16G1199000.v1.1
prunus_persica Prupe.1G020600_v2.0.a1
pyrus_communis pycom13g17260 pycom16g16760
rosa_laevigata RLG00000010105
rosa_multiflora Rmu_sc0003953.1_g000005 Rmu_sc0012183.1_g000004
rosa_roxburghii Rroxscaffold_5G00334310
rosa_rugosa Rorug03G0311700
rosa_samantha Rh4AG017300 Rh4BG012100 Rh4CG018500 Rh4DG013100
rosa_wichuraiana Rw4G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 172
Acc16I TGCGCA 1 cut(s) 138
AccB1I GGYRCC 1 cut(s) 689
AccB7I CCANNNNNTGG 1 cut(s) 1111
AccII CGCG 1 cut(s) 642
AciI CCGC 4 cut(s) 341, 640, 642, 764
AclWI GGATC 1 cut(s) 35
AcoI YGGCCR 2 cut(s) 631, 949
AcsI RAATTY 2 cut(s) 607, 1163
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 2 cut(s) 85, 214
AfiI CCNNNNNNNGG 4 cut(s) 734, 919, 1044, 1111
AgsI TTSAA 4 cut(s) 263, 578, 904, 1154
AluBI AGCT 9 cut(s) 380, 448, 509, 619, 668, 696, 998, 1082, 1150
AluI AGCT 9 cut(s) 380, 448, 509, 619, 668, 696, 998, 1082, 1150
Alw21I GWGCWC 1 cut(s) 874
Alw26I GTCTC 2 cut(s) 968, 1127
AlwI GGATC 1 cut(s) 35
AoxI GGCC 2 cut(s) 631, 949
ApeKI GCWGC 7 cut(s) 51, 266, 285, 288, 380, 403, 545
ApoI RAATTY 2 cut(s) 607, 1163
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 1 cut(s) 512
AsuHPI GGTGA 3 cut(s) 829, 848, 1106
AsuNHI GCTAGC 1 cut(s) 619
AvaII GGWCC 1 cut(s) 512
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BalI TGGCCA 1 cut(s) 951
BanI GGYRCC 1 cut(s) 689
BanII GRGCYC 1 cut(s) 1038
BbsI GAAGAC 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 874
BbvI GCAGC 7 cut(s) 63, 278, 297, 300, 367, 390, 532
BccI CCATC 4 cut(s) 212, 223, 808, 960
BceAI ACGGC 1 cut(s) 558
BcoDI GTCTC 2 cut(s) 968, 1127
BfaI CTAG 4 cut(s) 620, 665, 965, 1029
BglI GCCNNNNNGGC 1 cut(s) 645
BisI GCNGC 8 cut(s) 52, 267, 286, 289, 381, 404, 546, 640
BlsI GCNGC 8 cut(s) 53, 268, 287, 290, 382, 405, 547, 641
Bme18I GGWCC 1 cut(s) 512
BmgT120I GGNCC 1 cut(s) 512
BmiI GGNNCC 2 cut(s) 691, 1035
BmrI ACTGGG 2 cut(s) 389, 722
BmsI GCATC 3 cut(s) 76, 142, 272
BmtI GCTAGC 1 cut(s) 623
BmuI ACTGGG 2 cut(s) 389, 722
BpiI GAAGAC 1 cut(s) 241
BpmI CTGGAG 1 cut(s) 405
BsaAI YACGTR 1 cut(s) 1002
BsaI GGTCTC 1 cut(s) 968
BsaJI CCNNGG 3 cut(s) 412, 540, 640
BsaXI ACNNNNNCTCC 6 cut(s) 310, 340, 636, 666, 1047, 1077
Bsc4I CCNNNNNNNGG 4 cut(s) 734, 919, 1044, 1111
Bse118I RCCGGY 1 cut(s) 629
Bse1I ACTGG 5 cut(s) 395, 563, 691, 728, 767
Bse3DI GCAATG 2 cut(s) 86, 483
BseDI CCNNGG 3 cut(s) 412, 540, 640
BseGI GGATG 2 cut(s) 67, 287
BseLI CCNNNNNNNGG 4 cut(s) 734, 919, 1044, 1111
BseMI GCAATG 2 cut(s) 86, 483
BseMII CTCAG 2 cut(s) 458, 506
BseNI ACTGG 5 cut(s) 395, 563, 691, 728, 767
BseRI GAGGAG 6 cut(s) 169, 289, 315, 398, 750, 1057
BseXI GCAGC 7 cut(s) 63, 278, 297, 300, 367, 390, 532
Bsh1236I CGCG 1 cut(s) 642
BshFI GGCC 2 cut(s) 633, 951
BshNI GGYRCC 1 cut(s) 689
BsiHKAI GWGCWC 1 cut(s) 874
BsiSI CCGG 2 cut(s) 630, 913
BslI CCNNNNNNNGG 4 cut(s) 734, 919, 1044, 1111
BsmAI GTCTC 2 cut(s) 968, 1127
BsmI GAATGC 1 cut(s) 914
BsnI GGCC 2 cut(s) 633, 951
Bso31I GGTCTC 1 cut(s) 968
Bsp1286I GDGCHC 2 cut(s) 874, 1038
Bsp1407I TGTACA 1 cut(s) 83
Bsp143I GATC 3 cut(s) 40, 531, 796
Bsp19I CCATGG 1 cut(s) 412
BspACI CCGC 4 cut(s) 341, 640, 642, 764
BspANI GGCC 2 cut(s) 633, 951
BspCNI CTCAG 2 cut(s) 457, 505
BspFNI CGCG 1 cut(s) 642
BspLI GGNNCC 2 cut(s) 691, 1035
BspOI GCTAGC 1 cut(s) 623
BspPI GGATC 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 968
BsrDI GCAATG 2 cut(s) 86, 483
BsrFI RCCGGY 1 cut(s) 629
BsrGI TGTACA 1 cut(s) 83
BsrI ACTGG 5 cut(s) 395, 563, 691, 728, 767
BssAI RCCGGY 1 cut(s) 629
BssECI CCNNGG 3 cut(s) 412, 540, 640
BssMI GATC 3 cut(s) 40, 531, 796
BssT1I CCWWGG 1 cut(s) 412
BstAUI TGTACA 1 cut(s) 83
BstBAI YACGTR 1 cut(s) 1002
BstC8I GCNNGC 4 cut(s) 368, 617, 621, 631
BstDEI CTNAG 2 cut(s) 444, 492
BstDSI CCRYGG 3 cut(s) 412, 540, 640
BstF5I GGATG 2 cut(s) 67, 287
BstFNI CGCG 1 cut(s) 642
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 3 cut(s) 43, 534, 799
BstMAI GTCTC 2 cut(s) 968, 1127
BstMBI GATC 3 cut(s) 40, 531, 796
BstMWI GCNNNNNNNGC 3 cut(s) 639, 645, 1147
BstNSI RCATGY 2 cut(s) 370, 964
BstSNI TACGTA 1 cut(s) 1002
BstUI CGCG 1 cut(s) 642
BstV1I GCAGC 7 cut(s) 63, 278, 297, 300, 367, 390, 532
BstV2I GAAGAC 1 cut(s) 241
BsuRI GGCC 2 cut(s) 633, 951
BtgI CCRYGG 3 cut(s) 412, 540, 640
BtgZI GCGATG 1 cut(s) 445
BtsCI GGATG 2 cut(s) 67, 287
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 4 cut(s) 107, 402, 774, 816
Cac8I GCNNGC 4 cut(s) 368, 617, 621, 631
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 629
Cfr13I GGNCC 1 cut(s) 512
Cfr42I CCGCGG 1 cut(s) 643
Csp6I GTAC 2 cut(s) 84, 213
CviQI GTAC 2 cut(s) 84, 213
DdeI CTNAG 2 cut(s) 444, 492
DpnI GATC 3 cut(s) 42, 533, 798
DpnII GATC 3 cut(s) 40, 531, 796
DraI TTTAAA 1 cut(s) 15
DrdI GACNNNNNNGTC 1 cut(s) 172
DseDI GACNNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 2 cut(s) 631, 949
EciI GGCGGA 1 cut(s) 753
Eco105I TACGTA 1 cut(s) 1002
Eco130I CCWWGG 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 1038
Eco31I GGTCTC 1 cut(s) 968
Eco47I GGWCC 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 83
EcoT14I CCWWGG 1 cut(s) 412
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1038
ErhI CCWWGG 1 cut(s) 412
Fnu4HI GCNGC 8 cut(s) 52, 267, 286, 289, 381, 404, 546, 640
FokI GGATG 2 cut(s) 54, 294
FriOI GRGCYC 1 cut(s) 1038
Fsp4HI GCNGC 8 cut(s) 52, 267, 286, 289, 381, 404, 546, 640
FspBI CTAG 4 cut(s) 620, 665, 965, 1029
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 1 cut(s) 138
GluI GCNGC 8 cut(s) 52, 267, 286, 289, 381, 404, 546, 640
GsuI CTGGAG 1 cut(s) 405
HaeIII GGCC 2 cut(s) 633, 951
HapII CCGG 2 cut(s) 630, 913
HhaI GCGC 1 cut(s) 139
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 3 cut(s) 318, 733, 886
HindII GTYRAC 3 cut(s) 318, 733, 886
HindIII AAGCTT 1 cut(s) 1080
HinfI GANTC 1 cut(s) 90
HpaI GTTAAC 1 cut(s) 886
HpaII CCGG 2 cut(s) 630, 913
HphI GGTGA 3 cut(s) 829, 848, 1106
Hpy166II GTNNAC 4 cut(s) 318, 540, 733, 886
Hpy188I TCNGA 2 cut(s) 62, 348
Hpy188III TCNNGA 3 cut(s) 253, 800, 956
Hpy8I GTNNAC 4 cut(s) 318, 540, 733, 886
Hpy99I CGWCG 4 cut(s) 307, 310, 313, 316
HpyAV CCTTC 2 cut(s) 1085, 1118
HpyCH4IV ACGT 1 cut(s) 1001
HpyCH4V TGCA 8 cut(s) 4, 67, 79, 285, 548, 605, 662, 1014
HpyF10VI GCNNNNNNNGC 3 cut(s) 639, 645, 1147
HpyF3I CTNAG 2 cut(s) 444, 492
HpySE526I ACGT 1 cut(s) 1001
HspAI GCGC 1 cut(s) 137
KroI GCCGGC 1 cut(s) 629
KroNI GCCGGC 1 cut(s) 631
KspAI GTTAAC 1 cut(s) 886
KspI CCGCGG 1 cut(s) 643
Kzo9I GATC 3 cut(s) 40, 531, 796
LmnI GCTCC 3 cut(s) 411, 877, 1033
Lsp1109I GCAGC 7 cut(s) 63, 278, 297, 300, 367, 390, 532
LweI GCATC 3 cut(s) 76, 142, 272
MaeI CTAG 4 cut(s) 620, 665, 965, 1029
MaeII ACGT 1 cut(s) 1001
MalI GATC 3 cut(s) 42, 533, 798
MboI GATC 3 cut(s) 40, 531, 796
MboII GAAGA 7 cut(s) 180, 183, 186, 241, 413, 692, 892
MhlI GDGCHC 2 cut(s) 874, 1038
MlsI TGGCCA 1 cut(s) 951
MluCI AATT 3 cut(s) 607, 917, 1163
MluNI TGGCCA 1 cut(s) 951
MlyI GAGTC 1 cut(s) 99
MmeI TCCRAC 3 cut(s) 118, 453, 1033
Mox20I TGGCCA 1 cut(s) 951
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 629
MscI TGGCCA 1 cut(s) 951
MseI TTAA 6 cut(s) 14, 24, 611, 698, 885, 1161
MslI CAYNNNNRTG 1 cut(s) 809
Msp20I TGGCCA 1 cut(s) 951
MspA1I CMGCKG 1 cut(s) 642
MspI CCGG 2 cut(s) 630, 913
Mva1269I GAATGC 1 cut(s) 914
MvnI CGCG 1 cut(s) 642
MwoI GCNNNNNNNGC 3 cut(s) 639, 645, 1147
NaeI GCCGGC 1 cut(s) 631
NcoI CCATGG 1 cut(s) 412
NdeII GATC 3 cut(s) 40, 531, 796
NgoMIV GCCGGC 1 cut(s) 629
NheI GCTAGC 1 cut(s) 619
NlaIV GGNNCC 2 cut(s) 691, 1035
NsbI TGCGCA 1 cut(s) 138
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 370, 964
PaeI GCATGC 1 cut(s) 370
PcsI WCGNNNNNNNCGW 2 cut(s) 308, 311
PctI GAATGC 1 cut(s) 914
PdiI GCCGGC 1 cut(s) 631
PflMI CCANNNNNTGG 1 cut(s) 1111
PkrI GCNGC 8 cut(s) 53, 268, 287, 290, 382, 405, 547, 641
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Ppu21I YACGTR 1 cut(s) 1002
PspN4I GGNNCC 2 cut(s) 691, 1035
PspPI GGNCC 1 cut(s) 512
RsaI GTAC 2 cut(s) 85, 214
RsaNI GTAC 2 cut(s) 84, 213
RseI CAYNNNNRTG 1 cut(s) 809
SacII CCGCGG 1 cut(s) 643
SaqAI TTAA 6 cut(s) 14, 24, 611, 698, 885, 1161
SatI GCNGC 8 cut(s) 52, 267, 286, 289, 381, 404, 546, 640
Sau3AI GATC 3 cut(s) 40, 531, 796
Sau96I GGNCC 1 cut(s) 512
SchI GAGTC 1 cut(s) 99
SduI GDGCHC 2 cut(s) 874, 1038
SfaNI GCATC 3 cut(s) 76, 142, 272
Sfr303I CCGCGG 1 cut(s) 643
SgrBI CCGCGG 1 cut(s) 643
SinI GGWCC 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 809
SnaBI TACGTA 1 cut(s) 1002
SphI GCATGC 1 cut(s) 370
Sse9I AATT 3 cut(s) 607, 917, 1163
SsiI CCGC 4 cut(s) 341, 640, 642, 764
SspMI CTAG 4 cut(s) 620, 665, 965, 1029
StyI CCWWGG 1 cut(s) 412
TaiI ACGT 1 cut(s) 1004
TasI AATT 3 cut(s) 607, 917, 1163
TatI WGTACW 1 cut(s) 83
TauI GCSGC 1 cut(s) 642
Tru1I TTAA 6 cut(s) 14, 24, 611, 698, 885, 1161
Tru9I TTAA 6 cut(s) 14, 24, 611, 698, 885, 1161
TscAI CASTG 4 cut(s) 107, 402, 774, 816
TseI GCWGC 7 cut(s) 51, 266, 285, 288, 380, 403, 545
TspDTI ATGAA 5 cut(s) 466, 590, 645, 780, 1063
TspGWI ACGGA 1 cut(s) 750
TspRI CASTG 4 cut(s) 107, 402, 774, 816
Van91I CCANNNNNTGG 1 cut(s) 1111
VpaK11BI GGWCC 1 cut(s) 512
XapI RAATTY 2 cut(s) 607, 1163
XceI RCATGY 2 cut(s) 370, 964
XspI CTAG 4 cut(s) 620, 665, 965, 1029
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.