Rh4AG017300

Chlorophyllase-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011927
Physical Location & Seq
Forward (+)
3451985 .. 3454139
2155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG017300.1

Sequence Viewer

Length: 810 bp
ATGCTTTGTTTAGCAACAGTGACTCATATGCAGACAGTGTTCATATCCAATTTTCCTTCAAGTCACTCTCAATTATTAATGATTGCCTTACTCTGCAAGATAGAGGCTTTTAGGTTCTTTAAGTTGTATACAATAGCTGGATCAGATACAAGTGATGAGATCAAGTCCACAGCTGCAATAACAAACTGGTTATCAGAAGGACTTCAATCCTTGCTTCCACCCCATGTTCAAGCAAATCTAAACAAGCTAGCACTCGCAGGCCACAGCCGCGGAGGCAAAGTTTCTTTTGCAATAGCATTAGGGAAAGAAGGCATCACTACTAGCTTGAAATTTTCAGCCTTAATAGGCATAGACCCAGTTGATGGAATGGACACAGGGAAACAGACCCCTCCACCAGTACTAACCTATGTCCCTCATTCTTTCGATCTCGACGACATGGCAGTGATGGTGATTGGTACTGGTTTGGGTGAAGTGAAGAGAAACCCTCTATTCCCTGCCTGTGCTCCAAAGGGTGTTAACCATGAGGAGTTCTTCAAAGAGTGTCGAAAACCGGCTTGTTACTTTGTGGCCAAGGATTATGGGCATCTTGATATGCTTGATGATGAGACAAAGGGGATTAGAGGGAAGGCTACATATTGTTTGTGCAAAAATGGCAAGTCTAGGGAACCCATGAGAAAATTTGTTGGAGGAGTTGTGGTTGCTTTCGTTAAAGCCTATTTGGGAGGGGATATGAGTGAACTAATGGCTATAAGAGAAGGAGGGCATGAGGCTGCACCAATTGAGCTTCAAACTGTTGAATTCTTTTTGTGA

Protein Analysis

269

Amino Acids

29.27

Weight (kDa)

6.7

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chlorophyllase PF07224 41 - 267 2.6e-102 Chlorophyllase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0011927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43860
fragaria_vesca FvH4_4g00030 FvH4_4g01061 FvH4_4g01061
malus_domestica MD13G1198800.v1.1 MD16G1199000.v1.1
prunus_persica Prupe.1G020600_v2.0.a1
pyrus_communis pycom13g17260 pycom16g16760
rosa_laevigata RLG00000010105
rosa_multiflora Rmu_sc0003953.1_g000005 Rmu_sc0012183.1_g000004
rosa_roxburghii Rroxscaffold_5G00334310
rosa_rugosa Rorug03G0311700
rosa_samantha Rh4AG017300 Rh4BG012100 Rh4CG018500 Rh4DG013100
rosa_wichuraiana Rw4G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 362
AccI GTMKAC 1 cut(s) 128
AccII CGCG 1 cut(s) 270
AciI CCGC 2 cut(s) 268, 270
AclWI GGATC 1 cut(s) 148
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 3 cut(s) 329, 677, 797
AfaI GTAC 2 cut(s) 399, 457
AfiI CCNNNNNNNGG 1 cut(s) 362
AgsI TTSAA 7 cut(s) 60, 206, 230, 328, 535, 788, 797
AluBI AGCT 5 cut(s) 137, 173, 247, 324, 784
AluI AGCT 5 cut(s) 137, 173, 247, 324, 784
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 1 cut(s) 599
AlwI GGATC 1 cut(s) 148
AoxI GGCC 2 cut(s) 259, 567
ApeKI GCWGC 2 cut(s) 173, 770
ApoI RAATTY 3 cut(s) 329, 677, 797
AseI ATTAAT 1 cut(s) 77
Asp700I GAANNNNTTC 1 cut(s) 201
AsuHPI GGTGA 2 cut(s) 460, 479
AsuNHI GCTAGC 1 cut(s) 247
BalI TGGCCA 1 cut(s) 569
Bbv12I GWGCWC 1 cut(s) 505
BbvI GCAGC 2 cut(s) 160, 757
BccI CCATC 2 cut(s) 356, 439
BcoDI GTCTC 1 cut(s) 599
BfaI CTAG 3 cut(s) 248, 321, 660
BglI GCCNNNNNGGC 1 cut(s) 273
BisI GCNGC 3 cut(s) 174, 268, 771
BlsI GCNGC 3 cut(s) 175, 269, 772
BmcAI AGTACT 1 cut(s) 399
BmiI GGNNCC 1 cut(s) 666
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 2 cut(s) 321, 592
BmtI GCTAGC 1 cut(s) 251
BmuI ACTGGG 1 cut(s) 350
BplI GAGNNNNNCTC 2 cut(s) 469, 501
BsaJI CCNNGG 2 cut(s) 268, 570
BsaXI ACNNNNNCTCC 6 cut(s) 264, 294, 678, 681, 708, 711
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse118I RCCGGY 1 cut(s) 550
Bse1I ACTGG 4 cut(s) 191, 356, 395, 463
BseDI CCNNGG 2 cut(s) 268, 570
BseLI CCNNNNNNNGG 1 cut(s) 362
BseNI ACTGG 4 cut(s) 191, 356, 395, 463
BseRI GAGGAG 2 cut(s) 539, 702
BseXI GCAGC 2 cut(s) 160, 757
BsgI GTGCAG 1 cut(s) 756
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 2 cut(s) 261, 569
BsiHKAI GWGCWC 1 cut(s) 505
BsiSI CCGG 1 cut(s) 551
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 1 cut(s) 362
BsmAI GTCTC 1 cut(s) 599
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 2 cut(s) 261, 569
Bsp1286I GDGCHC 1 cut(s) 505
Bsp143I GATC 3 cut(s) 140, 159, 424
BspACI CCGC 2 cut(s) 268, 270
BspANI GGCC 2 cut(s) 261, 569
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 666
BspOI GCTAGC 1 cut(s) 251
BspPI GGATC 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 550
BsrI ACTGG 4 cut(s) 191, 356, 395, 463
BssAI RCCGGY 1 cut(s) 550
BssECI CCNNGG 2 cut(s) 268, 570
BssMI GATC 3 cut(s) 140, 159, 424
BssNAI GTATAC 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 570
Bst1107I GTATAC 1 cut(s) 129
Bst4CI ACNGT 3 cut(s) 19, 37, 793
Bst6I CTCTTC 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 249, 259
BstDSI CCRYGG 1 cut(s) 268
BstFNI CGCG 1 cut(s) 270
BstKTI GATC 3 cut(s) 143, 162, 427
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 3 cut(s) 140, 159, 424
BstMWI GCNNNNNNNGC 3 cut(s) 267, 273, 651
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 2 cut(s) 160, 757
BstZ17I GTATAC 1 cut(s) 129
BsuRI GGCC 2 cut(s) 261, 569
BtgI CCRYGG 1 cut(s) 268
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 3 cut(s) 24, 42, 447
Cac8I GCNNGC 2 cut(s) 249, 259
Cfr10I RCCGGY 1 cut(s) 550
Cfr42I CCGCGG 1 cut(s) 271
Csp6I GTAC 2 cut(s) 398, 456
CviAII CATG 5 cut(s) 224, 436, 521, 670, 764
CviQI GTAC 2 cut(s) 398, 456
DpnI GATC 3 cut(s) 142, 161, 426
DpnII GATC 3 cut(s) 140, 159, 424
EaeI YGGCCR 1 cut(s) 567
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 570
EcoRI GAATTC 1 cut(s) 797
EcoT14I CCWWGG 1 cut(s) 570
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 5 cut(s) 227, 439, 524, 673, 767
FaqI GGGAC 1 cut(s) 395
FatI CATG 5 cut(s) 223, 435, 520, 669, 763
FauNDI CATATG 1 cut(s) 27
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 3 cut(s) 174, 268, 771
Fsp4HI GCNGC 3 cut(s) 174, 268, 771
FspBI CTAG 3 cut(s) 248, 321, 660
GluI GCNGC 3 cut(s) 174, 268, 771
HaeIII GGCC 2 cut(s) 261, 569
HapII CCGG 1 cut(s) 551
Hin1II CATG 5 cut(s) 227, 439, 524, 673, 767
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HinfI GANTC 1 cut(s) 22
HpaI GTTAAC 1 cut(s) 517
HpaII CCGG 1 cut(s) 551
HphI GGTGA 2 cut(s) 460, 479
Hpy166II GTNNAC 4 cut(s) 129, 168, 517, 737
Hpy188I TCNGA 2 cut(s) 145, 196
Hpy188III TCNNGA 2 cut(s) 428, 587
Hpy8I GTNNAC 4 cut(s) 129, 168, 517, 737
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 5 cut(s) 66, 191, 302, 619, 749
HpyCH4III ACNGT 3 cut(s) 19, 37, 793
HpyCH4V TGCA 6 cut(s) 31, 96, 176, 290, 645, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 267, 273, 651
Hsp92II CATG 5 cut(s) 227, 439, 524, 673, 767
KspAI GTTAAC 1 cut(s) 517
KspI CCGCGG 1 cut(s) 271
Kzo9I GATC 3 cut(s) 140, 159, 424
LmnI GCTCC 1 cut(s) 508
Lsp1109I GCAGC 2 cut(s) 160, 757
LweI GCATC 2 cut(s) 321, 592
MaeI CTAG 3 cut(s) 248, 321, 660
MaeIII GTNAC 3 cut(s) 19, 62, 557
MalI GATC 3 cut(s) 142, 161, 426
MboI GATC 3 cut(s) 140, 159, 424
MboII GAAGA 2 cut(s) 487, 523
MfeI CAATTG 1 cut(s) 777
MhlI GDGCHC 1 cut(s) 505
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 6 cut(s) 49, 71, 329, 677, 777, 797
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 664
Mox20I TGGCCA 1 cut(s) 569
MroXI GAANNNNTTC 1 cut(s) 201
MscI TGGCCA 1 cut(s) 569
MseI TTAA 5 cut(s) 77, 120, 341, 516, 708
MslI CAYNNNNRTG 1 cut(s) 440
Msp20I TGGCCA 1 cut(s) 569
MspA1I CMGCKG 2 cut(s) 173, 270
MspI CCGG 1 cut(s) 551
MunI CAATTG 1 cut(s) 777
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 267, 273, 651
NdeI CATATG 1 cut(s) 27
NdeII GATC 3 cut(s) 140, 159, 424
NheI GCTAGC 1 cut(s) 247
NlaIII CATG 5 cut(s) 227, 439, 524, 673, 767
NlaIV GGNNCC 1 cut(s) 666
NmuCI GTSAC 2 cut(s) 19, 62
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PdmI GAANNNNTTC 1 cut(s) 201
PflMI CCANNNNNTGG 1 cut(s) 362
PkrI GCNGC 3 cut(s) 175, 269, 772
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PshBI ATTAAT 1 cut(s) 77
PspN4I GGNNCC 1 cut(s) 666
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 2 cut(s) 399, 457
RsaNI GTAC 2 cut(s) 398, 456
RseI CAYNNNNRTG 1 cut(s) 440
SacII CCGCGG 1 cut(s) 271
SaqAI TTAA 5 cut(s) 77, 120, 341, 516, 708
SatI GCNGC 3 cut(s) 174, 268, 771
Sau3AI GATC 3 cut(s) 140, 159, 424
ScaI AGTACT 1 cut(s) 399
SchI GAGTC 1 cut(s) 16
SduI GDGCHC 1 cut(s) 505
SetI ASST 7 cut(s) 116, 139, 175, 249, 326, 407, 786
SfaNI GCATC 2 cut(s) 321, 592
Sfr303I CCGCGG 1 cut(s) 271
SgrBI CCGCGG 1 cut(s) 271
SmiMI CAYNNNNRTG 1 cut(s) 440
Sse9I AATT 6 cut(s) 49, 71, 329, 677, 777, 797
SsiI CCGC 2 cut(s) 268, 270
SspMI CTAG 3 cut(s) 248, 321, 660
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 3 cut(s) 19, 37, 793
TaqI TCGA 3 cut(s) 423, 429, 544
TasI AATT 6 cut(s) 49, 71, 329, 677, 777, 797
TatI WGTACW 1 cut(s) 397
TauI GCSGC 1 cut(s) 270
Tru1I TTAA 5 cut(s) 77, 120, 341, 516, 708
Tru9I TTAA 5 cut(s) 77, 120, 341, 516, 708
TscAI CASTG 3 cut(s) 24, 42, 447
TseFI GTSAC 2 cut(s) 19, 62
TseI GCWGC 2 cut(s) 173, 770
Tsp45I GTSAC 2 cut(s) 19, 62
TspDTI ATGAA 1 cut(s) 31
TspRI CASTG 3 cut(s) 24, 42, 447
Van91I CCANNNNNTGG 1 cut(s) 362
VspI ATTAAT 1 cut(s) 77
XapI RAATTY 3 cut(s) 329, 677, 797
XmiI GTMKAC 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 3 cut(s) 248, 321, 660
ZrmI AGTACT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.