Rorug03G0311700

Chlorophyllase-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011927
Physical Location & Seq
Reverse (-)
33319307 .. 33320470
1164 bp
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UTR
Exon/CDS
Intron
Rorug03G0311700.1

Sequence Viewer

Length: 306 bp
ATGAGAATCTTGGTTACTGGAGGAGCTGGATTCATTGGTTCTCACCTGGTTGACAGATTAATGGAAAATGAAAAGAATGAGGTTATTGTTGTTGATAACTACTTCACTGGCTCCAAGGACAATCTTAAAAAATGGATTGGTCATCCCAGATTTGAGCTTATTCGTCATGATGTCACTGAAACATTGTTGGTTGAGGTTGATCGGATATACCATCTTGCATGCCCAGCTTCTCCAATTTTCTACAAATATAATCCTGTAAAGACAATAAAAACAAATGTGAGGGCAAGCGTGTTGCTGAGACCTTGA

Protein Analysis

101

Amino Acids

11.58

Weight (kDa)

9.17

Isoelectric Point (pI)

23.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 3 - 97 4.2e-13 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 3 - 95 9.1e-06 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 4 - 96 6.5e-13 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 4 - 93 6.6e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43860
fragaria_vesca FvH4_4g00030 FvH4_4g01061 FvH4_4g01061
malus_domestica MD13G1198800.v1.1 MD16G1199000.v1.1
prunus_persica Prupe.1G020600_v2.0.a1
pyrus_communis pycom13g17260 pycom16g16760
rosa_laevigata RLG00000010105
rosa_multiflora Rmu_sc0003953.1_g000005 Rmu_sc0012183.1_g000004
rosa_roxburghii Rroxscaffold_5G00334310
rosa_rugosa Rorug03G0311700
rosa_samantha Rh4AG017300 Rh4BG012100 Rh4CG018500 Rh4DG013100
rosa_wichuraiana Rw4G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AjnI CCWGG 1 cut(s) 45
AluBI AGCT 3 cut(s) 26, 157, 227
AluI AGCT 3 cut(s) 26, 157, 227
Alw26I GTCTC 1 cut(s) 292
AseI ATTAAT 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 35
BccI CCATC 1 cut(s) 219
BciT130I CCWGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 47
BmiI GGNNCC 1 cut(s) 112
BmrFI CCNGG 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 292
BsaJI CCNNGG 1 cut(s) 114
Bse1I ACTGG 2 cut(s) 22, 112
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 2 cut(s) 22, 112
BseRI GAGGAG 1 cut(s) 36
BseYI CCCAGC 1 cut(s) 223
BsmAI GTCTC 1 cut(s) 292
Bso31I GGTCTC 1 cut(s) 292
Bsp143I GATC 1 cut(s) 199
BspCNI CTCAG 1 cut(s) 288
BspHI TCATGA 1 cut(s) 166
BspLI GGNNCC 1 cut(s) 112
BspTNI GGTCTC 1 cut(s) 292
BsrI ACTGG 2 cut(s) 22, 112
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 1 cut(s) 47
BstC8I GCNNGC 2 cut(s) 220, 286
BstDEI CTNAG 1 cut(s) 296
BstF5I GGATG 1 cut(s) 142
BstKTI GATC 1 cut(s) 202
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 1 cut(s) 199
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 1 cut(s) 47
BstNSI RCATGY 1 cut(s) 222
BstSCI CCNGG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 142
BtsIMutI CAGTG 2 cut(s) 105, 174
Cac8I GCNNGC 2 cut(s) 220, 286
CciI TCATGA 1 cut(s) 166
CsiI ACCWGGT 1 cut(s) 45
CviAII CATG 2 cut(s) 167, 219
CviJI RGCY 4 cut(s) 26, 111, 157, 227
CviKI_1 RGCY 4 cut(s) 26, 111, 157, 227
DdeI CTNAG 1 cut(s) 296
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 114
Eco31I GGTCTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 2 cut(s) 170, 222
FaiI YATR 4 cut(s) 168, 208, 220, 249
FatI CATG 2 cut(s) 166, 218
FokI GGATG 1 cut(s) 129
GsaI CCCAGC 1 cut(s) 227
GsuI CTGGAG 1 cut(s) 39
Hin1II CATG 2 cut(s) 170, 222
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 2 cut(s) 6, 30
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 204
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 1 cut(s) 52
HpyCH4V TGCA 1 cut(s) 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 1 cut(s) 296
Hsp92II CATG 2 cut(s) 170, 222
Kzo9I GATC 1 cut(s) 199
LmnI GCTCC 2 cut(s) 23, 116
LpnPI CCDG 8 cut(s) 3, 12, 32, 59, 93, 160, 237, 267
MabI ACCWGGT 1 cut(s) 45
MaeIII GTNAC 2 cut(s) 13, 172
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MluCI AATT 1 cut(s) 234
MnlI CCTC 4 cut(s) 14, 73, 187, 273
MseI TTAA 2 cut(s) 59, 126
MspR9I CCNGG 1 cut(s) 47
MvaI CCWGG 1 cut(s) 47
MwoI GCNNNNNNNGC 1 cut(s) 224
NdeII GATC 1 cut(s) 199
NlaIII CATG 2 cut(s) 170, 222
NlaIV GGNNCC 1 cut(s) 112
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 1 cut(s) 222
PaeI GCATGC 1 cut(s) 222
PagI TCATGA 1 cut(s) 166
PfeI GAWTC 2 cut(s) 6, 30
PshBI ATTAAT 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 45
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 112
SaqAI TTAA 2 cut(s) 59, 126
Sau3AI GATC 1 cut(s) 199
ScrFI CCNGG 1 cut(s) 47
SetI ASST 7 cut(s) 28, 48, 84, 159, 198, 229, 304
SexAI ACCWGGT 1 cut(s) 45
SphI GCATGC 1 cut(s) 222
Sse9I AATT 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 1 cut(s) 114
TasI AATT 1 cut(s) 234
TfiI GAWTC 2 cut(s) 6, 30
Tru1I TTAA 2 cut(s) 59, 126
Tru9I TTAA 2 cut(s) 59, 126
TscAI CASTG 2 cut(s) 112, 181
TseFI GTSAC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 2 cut(s) 22, 84
TspRI CASTG 2 cut(s) 112, 181
VspI ATTAAT 1 cut(s) 59
XceI RCATGY 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.