Rw4G001110

Chlorophyllase-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011927
Physical Location & Seq
Forward (+)
2141837 .. 2143726
1890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001110.1

Sequence Viewer

Length: 963 bp
ATGTCTACTTCATCTTGTTCAAATGTTTTTGAGCTTGGAAAGTACAGGGTAGAACTCCAAACAGTTGAGGCAGCAGAGTCTAGTAGATGCTGCAGCACCAAGTCTTCTTCAAGTGTTTCGGTTCCGCCTCCGAAACCGCTTTTGATTGGCTTGCCTTGTGAAGCTGGAGAGTTCCCAGTGCTGCTTCTCCTCCATGGTTTCCTTCTCTCCAACTCTTTCTACTCTCAGCTTATCCAACACGTTGCTTCTCATGGCTTCATCGTCATTGCTCCTCAGTTGTATACAATAGCTGGATCAGATACAAGTGATGAGATAAAGTCCACAGCTGCAATAACAAACTGGTTATCAGAAGGACTTCAATCCTTGCTTCCACCCCATGTTCAAGCAAATCTAAACAAGCTAGCACTCGCAGGCCACAGCCGCGGAGGCAAAGTTTCTTTTGCTATAGCATTAGGGAAAGAAGGCATCACTACTAGCTTGAAATTTTCAGCCTTAATAGGCATAGACCCAGTTGATGGAATGGACACAGGGAAACAGACCCCTCCACCAGTACTAACCTATGTCCCTCATTCTTTCGATCTCGACGACATGGCAGTGATGGTGATTGGTACTGGTTTGGGTGAAGTGAAGAGAAACCCTCTATTCCCTGCCTGTGCTCCAAAGGGTGTTAACCATGAGGAGTTCTTCAAAGAGTGTCGAAAACCGGCTTGTTACTTTGTGGCCAAGGATTATGGGCATCTTGATATGCTTGATGATGAGACAAAGGGGATTAGAGGGAAGGCTACATATTGTTTGTGCAAAAATGGCAAGTCTAGGGAACCCATGAGAAAATTTGTTGGAGGAGTTGTGGTTGCTTTCGTTAAAGCCTATTTGGGAGGGGATATGAGTGAACTAATGGCTATAAGAGAAGGAGGGCATGAGGCTGCACCAATTGAGCTTCAAACTGTTGAATTCTTTTTGTGA

Protein Analysis

320

Amino Acids

34.42

Weight (kDa)

6.15

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chlorophyllase PF07224 6 - 318 8.4e-137 Chlorophyllase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0011927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43860
fragaria_vesca FvH4_4g00030 FvH4_4g01061 FvH4_4g01061
malus_domestica MD13G1198800.v1.1 MD16G1199000.v1.1
prunus_persica Prupe.1G020600_v2.0.a1
pyrus_communis pycom13g17260 pycom16g16760
rosa_laevigata RLG00000010105
rosa_multiflora Rmu_sc0003953.1_g000005 Rmu_sc0012183.1_g000004
rosa_roxburghii Rroxscaffold_5G00334310
rosa_rugosa Rorug03G0311700
rosa_samantha Rh4AG017300 Rh4BG012100 Rh4CG018500 Rh4DG013100
rosa_wichuraiana Rw4G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 515
AccI GTMKAC 2 cut(s) 5, 281
AccII CGCG 1 cut(s) 423
AciI CCGC 4 cut(s) 125, 137, 421, 423
AclWI GGATC 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 720
AcsI RAATTY 3 cut(s) 482, 830, 950
AfaI GTAC 3 cut(s) 44, 552, 610
AfiI CCNNNNNNNGG 1 cut(s) 515
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 8 cut(s) 21, 111, 359, 383, 481, 688, 941, 950
AluBI AGCT 8 cut(s) 34, 164, 229, 290, 326, 400, 477, 937
AluI AGCT 8 cut(s) 34, 164, 229, 290, 326, 400, 477, 937
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 1 cut(s) 752
AlwI GGATC 1 cut(s) 301
AoxI GGCC 2 cut(s) 412, 720
ApeKI GCWGC 6 cut(s) 71, 90, 93, 181, 326, 923
ApoI RAATTY 3 cut(s) 482, 830, 950
Asp700I GAANNNNTTC 1 cut(s) 354
AsuHPI GGTGA 2 cut(s) 613, 632
AsuNHI GCTAGC 1 cut(s) 400
BalI TGGCCA 1 cut(s) 722
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 6 cut(s) 77, 83, 105, 168, 313, 910
BccI CCATC 2 cut(s) 509, 592
BcoDI GTCTC 1 cut(s) 752
BfaI CTAG 4 cut(s) 81, 401, 474, 813
BfmI CTRYAG 2 cut(s) 91, 444
BglI GCCNNNNNGGC 1 cut(s) 426
BisI GCNGC 7 cut(s) 72, 91, 94, 182, 327, 421, 924
BlsI GCNGC 7 cut(s) 73, 92, 95, 183, 328, 422, 925
BmcAI AGTACT 1 cut(s) 552
BmiI GGNNCC 2 cut(s) 123, 819
BmrI ACTGGG 2 cut(s) 170, 503
BmsI GCATC 3 cut(s) 77, 474, 745
BmtI GCTAGC 1 cut(s) 404
BmuI ACTGGG 2 cut(s) 170, 503
BpiI GAAGAC 1 cut(s) 96
BplI GAGNNNNNCTC 2 cut(s) 622, 654
BpmI CTGGAG 1 cut(s) 186
BsaJI CCNNGG 3 cut(s) 193, 421, 723
BsaXI ACNNNNNCTCC 6 cut(s) 417, 447, 831, 834, 861, 864
Bsc4I CCNNNNNNNGG 1 cut(s) 515
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 5 cut(s) 176, 344, 509, 548, 616
Bse3DI GCAATG 1 cut(s) 264
BseDI CCNNGG 3 cut(s) 193, 421, 723
BseLI CCNNNNNNNGG 1 cut(s) 515
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 239, 287
BseNI ACTGG 5 cut(s) 176, 344, 509, 548, 616
BseRI GAGGAG 4 cut(s) 179, 261, 692, 855
BseXI GCAGC 6 cut(s) 77, 83, 105, 168, 313, 910
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 1 cut(s) 423
BshFI GGCC 2 cut(s) 414, 722
BsiHKAI GWGCWC 1 cut(s) 658
BsiSI CCGG 1 cut(s) 704
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 1 cut(s) 515
BsmAI GTCTC 1 cut(s) 752
BsmFI GGGAC 1 cut(s) 548
BsnI GGCC 2 cut(s) 414, 722
Bsp1286I GDGCHC 1 cut(s) 658
Bsp143I GATC 2 cut(s) 293, 577
Bsp19I CCATGG 1 cut(s) 193
BspACI CCGC 4 cut(s) 125, 137, 421, 423
BspANI GGCC 2 cut(s) 414, 722
BspCNI CTCAG 2 cut(s) 238, 286
BspFNI CGCG 1 cut(s) 423
BspLI GGNNCC 2 cut(s) 123, 819
BspMAI CTGCAG 1 cut(s) 95
BspOI GCTAGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 264
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 5 cut(s) 176, 344, 509, 548, 616
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 3 cut(s) 193, 421, 723
BssMI GATC 2 cut(s) 293, 577
BssNAI GTATAC 1 cut(s) 282
BssT1I CCWWGG 2 cut(s) 193, 723
Bst1107I GTATAC 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 64, 946
Bst6I CTCTTC 1 cut(s) 623
BstC8I GCNNGC 3 cut(s) 152, 402, 412
BstDEI CTNAG 2 cut(s) 225, 273
BstDSI CCRYGG 2 cut(s) 193, 421
BstFNI CGCG 1 cut(s) 423
BstKTI GATC 2 cut(s) 296, 580
BstMAI GTCTC 1 cut(s) 752
BstMBI GATC 2 cut(s) 293, 577
BstMWI GCNNNNNNNGC 3 cut(s) 420, 426, 804
BstSFI CTRYAG 2 cut(s) 91, 444
BstUI CGCG 1 cut(s) 423
BstV1I GCAGC 6 cut(s) 77, 83, 105, 168, 313, 910
BstV2I GAAGAC 1 cut(s) 96
BstZ17I GTATAC 1 cut(s) 282
BsuRI GGCC 2 cut(s) 414, 722
BtgI CCRYGG 2 cut(s) 193, 421
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 2 cut(s) 183, 600
Cac8I GCNNGC 3 cut(s) 152, 402, 412
Cfr10I RCCGGY 1 cut(s) 703
Cfr42I CCGCGG 1 cut(s) 424
Csp6I GTAC 3 cut(s) 43, 551, 609
CviAII CATG 7 cut(s) 194, 251, 377, 589, 674, 823, 917
CviQI GTAC 3 cut(s) 43, 551, 609
DdeI CTNAG 2 cut(s) 225, 273
DpnI GATC 2 cut(s) 295, 579
DpnII GATC 2 cut(s) 293, 577
EaeI YGGCCR 1 cut(s) 720
Eam1104I CTCTTC 1 cut(s) 623
EarI CTCTTC 1 cut(s) 623
EciI GGCGGA 1 cut(s) 114
Eco130I CCWWGG 2 cut(s) 193, 723
EcoRI GAATTC 1 cut(s) 950
EcoT14I CCWWGG 2 cut(s) 193, 723
ErhI CCWWGG 2 cut(s) 193, 723
FaeI CATG 7 cut(s) 197, 254, 380, 592, 677, 826, 920
FaqI GGGAC 1 cut(s) 548
FatI CATG 7 cut(s) 193, 250, 376, 588, 673, 822, 916
FblI GTMKAC 2 cut(s) 5, 281
Fnu4HI GCNGC 7 cut(s) 72, 91, 94, 182, 327, 421, 924
Fsp4HI GCNGC 7 cut(s) 72, 91, 94, 182, 327, 421, 924
FspBI CTAG 4 cut(s) 81, 401, 474, 813
GluI GCNGC 7 cut(s) 72, 91, 94, 182, 327, 421, 924
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 2 cut(s) 414, 722
HapII CCGG 1 cut(s) 704
Hin1II CATG 7 cut(s) 197, 254, 380, 592, 677, 826, 920
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HinfI GANTC 1 cut(s) 77
HpaI GTTAAC 1 cut(s) 670
HpaII CCGG 1 cut(s) 704
HphI GGTGA 2 cut(s) 613, 632
Hpy166II GTNNAC 5 cut(s) 6, 282, 321, 670, 890
Hpy188I TCNGA 3 cut(s) 132, 298, 349
Hpy188III TCNNGA 2 cut(s) 581, 740
Hpy8I GTNNAC 5 cut(s) 6, 282, 321, 670, 890
Hpy99I CGWCG 1 cut(s) 587
HpyAV CCTTC 5 cut(s) 212, 344, 455, 772, 902
HpyCH4III ACNGT 2 cut(s) 64, 946
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 4 cut(s) 93, 329, 798, 926
HpyF10VI GCNNNNNNNGC 3 cut(s) 420, 426, 804
HpyF3I CTNAG 2 cut(s) 225, 273
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 7 cut(s) 197, 254, 380, 592, 677, 826, 920
KspAI GTTAAC 1 cut(s) 670
KspI CCGCGG 1 cut(s) 424
Kzo9I GATC 2 cut(s) 293, 577
LmnI GCTCC 2 cut(s) 274, 661
Lsp1109I GCAGC 6 cut(s) 77, 83, 105, 168, 313, 910
LweI GCATC 3 cut(s) 77, 474, 745
MaeI CTAG 4 cut(s) 81, 401, 474, 813
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 710
MalI GATC 2 cut(s) 295, 579
MboI GATC 2 cut(s) 293, 577
MboII GAAGA 4 cut(s) 96, 99, 640, 676
MfeI CAATTG 1 cut(s) 930
MhlI GDGCHC 1 cut(s) 658
MlsI TGGCCA 1 cut(s) 722
MluCI AATT 4 cut(s) 482, 830, 930, 950
MluNI TGGCCA 1 cut(s) 722
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 3 cut(s) 234, 259, 817
Mox20I TGGCCA 1 cut(s) 722
MroXI GAANNNNTTC 1 cut(s) 354
MscI TGGCCA 1 cut(s) 722
MseI TTAA 3 cut(s) 494, 669, 861
MslI CAYNNNNRTG 1 cut(s) 593
Msp20I TGGCCA 1 cut(s) 722
MspA1I CMGCKG 2 cut(s) 326, 423
MspI CCGG 1 cut(s) 704
MunI CAATTG 1 cut(s) 930
MvnI CGCG 1 cut(s) 423
MwoI GCNNNNNNNGC 3 cut(s) 420, 426, 804
NcoI CCATGG 1 cut(s) 193
NdeII GATC 2 cut(s) 293, 577
NheI GCTAGC 1 cut(s) 400
NlaIII CATG 7 cut(s) 197, 254, 380, 592, 677, 826, 920
NlaIV GGNNCC 2 cut(s) 123, 819
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PdmI GAANNNNTTC 1 cut(s) 354
PflMI CCANNNNNTGG 1 cut(s) 515
PkrI GCNGC 7 cut(s) 73, 92, 95, 183, 328, 422, 925
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PspN4I GGNNCC 2 cut(s) 123, 819
PstI CTGCAG 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 3 cut(s) 44, 552, 610
RsaNI GTAC 3 cut(s) 43, 551, 609
RseI CAYNNNNRTG 1 cut(s) 593
SacII CCGCGG 1 cut(s) 424
SaqAI TTAA 3 cut(s) 494, 669, 861
SatI GCNGC 7 cut(s) 72, 91, 94, 182, 327, 421, 924
Sau3AI GATC 2 cut(s) 293, 577
ScaI AGTACT 1 cut(s) 552
SchI GAGTC 1 cut(s) 86
SduI GDGCHC 1 cut(s) 658
SfaNI GCATC 3 cut(s) 77, 474, 745
SfcI CTRYAG 2 cut(s) 91, 444
Sfr303I CCGCGG 1 cut(s) 424
SgrBI CCGCGG 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 593
Sse9I AATT 4 cut(s) 482, 830, 930, 950
SsiI CCGC 4 cut(s) 125, 137, 421, 423
SspMI CTAG 4 cut(s) 81, 401, 474, 813
StyI CCWWGG 2 cut(s) 193, 723
TaaI ACNGT 2 cut(s) 64, 946
TaiI ACGT 1 cut(s) 243
TaqI TCGA 3 cut(s) 576, 582, 697
TasI AATT 4 cut(s) 482, 830, 930, 950
TatI WGTACW 2 cut(s) 42, 550
TauI GCSGC 1 cut(s) 423
Tru1I TTAA 3 cut(s) 494, 669, 861
Tru9I TTAA 3 cut(s) 494, 669, 861
TscAI CASTG 2 cut(s) 183, 600
TseI GCWGC 6 cut(s) 71, 90, 93, 181, 326, 923
TspDTI ATGAA 1 cut(s) 247
TspRI CASTG 2 cut(s) 183, 600
Van91I CCANNNNNTGG 1 cut(s) 515
XapI RAATTY 3 cut(s) 482, 830, 950
XmiI GTMKAC 2 cut(s) 5, 281
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 4 cut(s) 81, 401, 474, 813
ZrmI AGTACT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.