pycom13g17840

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007628
Physical Location & Seq
Reverse (-)
13737192 .. 13737728
537 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g17840.1

Sequence Viewer

Length: 537 bp
ATGTCCGCTGGATTGATTTCCCAGTTCAAGGAAGCGACACAAACGGCAAGGGCCACGCTCAGGCCATGGGGTGAGCTCCTGGAGCCCACCTCACTCAGCCTCCCCCCCAGTCTGTCTGACGCGACGACCCGACTCGCCCAGAACTTGACCCACTTCCGCTCCAACTACGCCCTGATCGCCCTGATCGTTCTCTTCCTCAGCCTCCTCTACCACCCGTTCTCCATCATCGTCTTCTTGATTGTCTTCGCCGCGTGGCTCGTGCTCTACTTCTCGCGTGACCAGCCCCTCGAGGTGTTCGGGTTTGTGGTCCCGGACCGAGTCGTGATGGTTGTTCTTGCGGTGGTGACGGTGGTGGCTCTGGTCCTGACTCACGTGTGGGTCAACGTCGTCGTTTCGGGGGTGATTGGGGTCGTTTTGATTGGGCTGCATGCGGTGTTTAGGGGGACGGAGGATCTTGTTATGGACGATCAGGAATCGCCGTATGGGACTCTGCTCTCGGATGATGCTGACCCAAGTGGGAATTACACCATTATGTGA

Protein Analysis

179

Amino Acids

19.53

Weight (kDa)

4.8

Isoelectric Point (pI)

27.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 17 - 156 1.8e-47 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 287
AccBSI CCGCTC 1 cut(s) 159
AccII CGCG 3 cut(s) 122, 251, 274
AciI CCGC 5 cut(s) 6, 157, 249, 338, 431
AclWI GGATC 1 cut(s) 459
AcvI CACGTG 1 cut(s) 373
AfiI CCNNNNNNNGG 2 cut(s) 28, 60
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 1 cut(s) 28
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw21I GWGCWC 2 cut(s) 78, 264
AlwI GGATC 1 cut(s) 459
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 2 cut(s) 51, 62
ApeKI GCWGC 1 cut(s) 424
AspS9I GGNCC 4 cut(s) 51, 307, 313, 361
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 3 cut(s) 83, 355, 412
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 3 cut(s) 307, 313, 361
BanII GRGCYC 2 cut(s) 78, 87
BauI CACGAG 1 cut(s) 257
BbrPI CACGTG 1 cut(s) 373
BbsI GAAGAC 2 cut(s) 223, 235
Bbv12I GWGCWC 2 cut(s) 78, 264
BbvCI CCTCAGC 1 cut(s) 197
BbvI GCAGC 1 cut(s) 411
BccI CCATC 2 cut(s) 230, 319
BceAI ACGGC 2 cut(s) 60, 463
BciT130I CCWGG 1 cut(s) 80
BcnI CCSGG 1 cut(s) 311
BisI GCNGC 2 cut(s) 249, 425
BlsI GCNGC 2 cut(s) 250, 426
Bme1390I CCNGG 2 cut(s) 80, 311
Bme18I GGWCC 3 cut(s) 307, 313, 361
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 4 cut(s) 51, 307, 313, 361
BmiI GGNNCC 2 cut(s) 84, 309
BmrFI CCNGG 2 cut(s) 80, 311
BmrI ACTGGG 2 cut(s) 16, 102
BmsI GCATC 1 cut(s) 493
BmuI ACTGGG 2 cut(s) 16, 102
BpiI GAAGAC 2 cut(s) 223, 235
BplI GAGNNNNNCTC 2 cut(s) 74, 106
BpmI CTGGAG 1 cut(s) 101
Bpu10I CCTNAGC 2 cut(s) 59, 197
BpuMI CCSGG 1 cut(s) 311
BsaAI YACGTR 1 cut(s) 373
BsaJI CCNNGG 1 cut(s) 65
BsaXI ACNNNNNCTCC 6 cut(s) 84, 114, 143, 173, 203, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 60
Bse1I ACTGG 2 cut(s) 22, 108
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 1 cut(s) 65
BseGI GGATG 1 cut(s) 505
BseLI CCNNNNNNNGG 2 cut(s) 28, 60
BseMII CTCAG 3 cut(s) 73, 109, 211
BseNI ACTGG 2 cut(s) 22, 108
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 1 cut(s) 411
Bsh1236I CGCG 3 cut(s) 122, 251, 274
BshFI GGCC 2 cut(s) 53, 64
BsiHKAI GWGCWC 2 cut(s) 78, 264
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 3 cut(s) 293, 457, 499
BslI CCNNNNNNNGG 2 cut(s) 28, 60
BsmFI GGGAC 3 cut(s) 293, 457, 499
BsnI GGCC 2 cut(s) 53, 64
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 3 cut(s) 78, 87, 264
Bsp143I GATC 4 cut(s) 174, 183, 451, 466
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 5 cut(s) 6, 157, 249, 338, 431
BspANI GGCC 2 cut(s) 53, 64
BspCNI CTCAG 3 cut(s) 72, 108, 210
BspFNI CGCG 3 cut(s) 122, 251, 274
BspLI GGNNCC 2 cut(s) 84, 309
BspPI GGATC 1 cut(s) 459
BsrBI CCGCTC 1 cut(s) 159
BsrI ACTGG 2 cut(s) 22, 108
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 4 cut(s) 174, 183, 451, 466
BssSI CACGAG 1 cut(s) 257
BssT1I CCWWGG 1 cut(s) 65
Bst2BI CACGAG 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 1 cut(s) 197
BstBAI YACGTR 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 3 cut(s) 59, 95, 197
BstDSI CCRYGG 1 cut(s) 65
BstF5I GGATG 1 cut(s) 505
BstFNI CGCG 3 cut(s) 122, 251, 274
BstKTI GATC 4 cut(s) 177, 186, 454, 469
BstMBI GATC 4 cut(s) 174, 183, 451, 466
BstMWI GCNNNNNNNGC 3 cut(s) 82, 176, 280
BstNI CCWGG 1 cut(s) 80
BstNSI RCATGY 1 cut(s) 431
BstSCI CCNGG 2 cut(s) 78, 309
BstUI CGCG 3 cut(s) 122, 251, 274
BstV1I GCAGC 1 cut(s) 411
BstV2I GAAGAC 2 cut(s) 223, 235
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BsuRI GGCC 2 cut(s) 53, 64
BtgI CCRYGG 1 cut(s) 65
BtsCI GGATG 1 cut(s) 505
Cac8I GCNNGC 1 cut(s) 429
Cfr13I GGNCC 4 cut(s) 51, 307, 313, 361
CpoI CGGWCCG 1 cut(s) 313
CseI GACGC 1 cut(s) 128
CspI CGGWCCG 1 cut(s) 313
CviAII CATG 2 cut(s) 66, 428
DdeI CTNAG 3 cut(s) 59, 95, 197
DpnI GATC 4 cut(s) 176, 185, 453, 468
DpnII GATC 4 cut(s) 174, 183, 451, 466
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 65
Eco24I GRGCYC 2 cut(s) 78, 87
Eco47I GGWCC 3 cut(s) 307, 313, 361
Eco53kI GAGCTC 1 cut(s) 76
Eco72I CACGTG 1 cut(s) 373
Eco88I CYCGRG 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 65
EcoT38I GRGCYC 2 cut(s) 78, 87
ErhI CCWWGG 1 cut(s) 65
FaeI CATG 2 cut(s) 69, 431
FaiI YATR 5 cut(s) 67, 429, 461, 483, 533
FaqI GGGAC 3 cut(s) 293, 457, 499
FatI CATG 2 cut(s) 65, 427
Fnu4HI GCNGC 2 cut(s) 249, 425
FokI GGATG 1 cut(s) 512
FriOI GRGCYC 2 cut(s) 78, 87
Fsp4HI GCNGC 2 cut(s) 249, 425
GluI GCNGC 2 cut(s) 249, 425
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 2 cut(s) 53, 64
HapII CCGG 1 cut(s) 311
HgaI GACGC 1 cut(s) 128
Hin1II CATG 2 cut(s) 69, 431
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 5 cut(s) 132, 318, 367, 473, 487
HpaII CCGG 1 cut(s) 311
HphI GGTGA 3 cut(s) 83, 355, 412
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 2 cut(s) 118, 499
Hpy188III TCNNGA 4 cut(s) 235, 322, 364, 470
Hpy8I GTNNAC 1 cut(s) 382
Hpy99I CGWCG 3 cut(s) 127, 389, 392
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 2 cut(s) 372, 384
HpyCH4V TGCA 1 cut(s) 427
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 176, 280
HpyF3I CTNAG 3 cut(s) 59, 95, 197
HpySE526I ACGT 2 cut(s) 372, 384
Hsp92II CATG 2 cut(s) 69, 431
Kzo9I GATC 4 cut(s) 174, 183, 451, 466
LmnI GCTCC 3 cut(s) 81, 82, 164
Lsp1109I GCAGC 1 cut(s) 411
LweI GCATC 1 cut(s) 493
MaeII ACGT 2 cut(s) 372, 384
MaeIII GTNAC 2 cut(s) 275, 343
MalI GATC 4 cut(s) 176, 185, 453, 468
MbiI CCGCTC 1 cut(s) 159
MboI GATC 4 cut(s) 174, 183, 451, 466
MboII GAAGA 3 cut(s) 184, 223, 235
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 3 cut(s) 78, 87, 264
MluCI AATT 1 cut(s) 520
MlyI GAGTC 4 cut(s) 126, 327, 361, 481
MmeI TCCRAC 1 cut(s) 186
MnlI CCTC 8 cut(s) 100, 110, 206, 212, 215, 283, 296, 442
MslI CAYNNNNRTG 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 2 cut(s) 80, 311
MvaI CCWGG 1 cut(s) 80
MvnI CGCG 3 cut(s) 122, 251, 274
MwoI GCNNNNNNNGC 3 cut(s) 82, 176, 280
NciI CCSGG 1 cut(s) 311
NcoI CCATGG 1 cut(s) 65
NdeII GATC 4 cut(s) 174, 183, 451, 466
NlaIII CATG 2 cut(s) 69, 431
NlaIV GGNNCC 2 cut(s) 84, 309
NmuCI GTSAC 2 cut(s) 275, 343
NspI RCATGY 1 cut(s) 431
PaeI GCATGC 1 cut(s) 431
PaeR7I CTCGAG 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PfeI GAWTC 1 cut(s) 473
PflFI GACNNNGTC 1 cut(s) 317
PfoI TCCNGGA 2 cut(s) 78, 309
PkrI GCNGC 2 cut(s) 250, 426
PleI GAGTC 4 cut(s) 126, 326, 361, 481
PmaCI CACGTG 1 cut(s) 373
PmlI CACGTG 1 cut(s) 373
PpsI GAGTC 4 cut(s) 126, 326, 361, 481
Ppu21I YACGTR 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 78
PspCI CACGTG 1 cut(s) 373
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 2 cut(s) 84, 309
PspPI GGNCC 4 cut(s) 51, 307, 313, 361
PspXI VCTCGAGB 1 cut(s) 287
PsuI RGATCY 1 cut(s) 451
PsyI GACNNNGTC 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 530
Rsr2I CGGWCCG 1 cut(s) 313
RsrII CGGWCCG 1 cut(s) 313
SacI GAGCTC 1 cut(s) 78
SatI GCNGC 2 cut(s) 249, 425
Sau3AI GATC 4 cut(s) 174, 183, 451, 466
Sau96I GGNCC 4 cut(s) 51, 307, 313, 361
SchI GAGTC 4 cut(s) 126, 327, 361, 481
ScrFI CCNGG 2 cut(s) 80, 311
SduI GDGCHC 3 cut(s) 78, 87, 264
SetI ASST 5 cut(s) 78, 92, 294, 375, 387
SfaNI GCATC 1 cut(s) 493
Sfr274I CTCGAG 1 cut(s) 287
SinI GGWCC 3 cut(s) 307, 313, 361
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SphI GCATGC 1 cut(s) 431
Sse9I AATT 1 cut(s) 520
SsiI CCGC 5 cut(s) 6, 157, 249, 338, 431
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 78, 309
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 2 cut(s) 375, 387
TaqI TCGA 1 cut(s) 288
TaqII GACCGA 1 cut(s) 330
TasI AATT 1 cut(s) 520
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 1 cut(s) 473
TseFI GTSAC 2 cut(s) 275, 343
TseI GCWGC 1 cut(s) 424
Tsp45I GTSAC 2 cut(s) 275, 343
TspGWI ACGGA 1 cut(s) 461
Tth111I GACNNNGTC 1 cut(s) 317
VpaK11BI GGWCC 3 cut(s) 307, 313, 361
XceI RCATGY 1 cut(s) 431
XhoI CTCGAG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.