pycom13g22400

Syntaxin 6, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009458
Physical Location & Seq
Forward (+)
19747235 .. 19750358
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1032 bp
ATGATGGTCGCAAGCAGTTTTGATCTTTGGAAAAAGGACGTCTTCTTTCCTGCAGCTGAGGAGGTCCAAGAATCAGCTGACATAATGGAATCTACGTACAGGGTATGGGTTCGACAGAGGAGTGAAAGGCTAGCACCAAAACATTTGGATGAACTCTGTAGGGAGCTGCAAACTGCTTTAGGTACTGCCAAATGGCAGTTGGAAGAGTTTGAGAAGGCTGTCAGATTGAGCTATGGACAACATGGTGATGATCATACAGCAGCTAGACATGGACAATTTATTACTGCTATTGAAAACCAAATTTCCCAAGTTGAAACAGCACTAAGGGAATCGTTTAGTGTGGAAGGAAAGCAACCCCTTCGATGGGTACAGCTGGATGAAGAAGAACATGATGATTTTGCTGCATTTCTCTCTGGGACATCCCAAAGTATGCAAACTGCCAAAAATGAATGTCTAGAACTAGGACCTTGTAGGAGCAGCTCTGGGGAAAGTGACATTAGAAGAAAAGAGATGAACCTTAAATCTAGTGCTGCATGCAATGGAGACATCTCTGATGAAATAAAGGGTGTTAAAGATGTTATTACTCTTTCCAAGGATCCAAATTTCGTCATGGAATCGAAAGGAAAAGAAATTGCAGGAATGAGGGATGATATATTTTATGATACAGAAAAAACAACTAATACAAGGAAGGCATGCAGCTCACCAAATTATGGTGAGCTGAGGATTGTAATAGCGGATGAAGTTGAACAGAGAAGAAATCTAATTCCGGGATATGAGGACACACCCAAAGAAAAACGATCTAAACTTGTCTTCTGGAGGCAAAAGTGTGGGCAATTGCATCTGGGAACTGGAGCAGTTAATTTGTTCAACCAGATGTTTGGTCGGGTTGGTGCATCTCAGAAGCAATTGCAAAGTCCACGGAAGCAATGGCAGACACCACTACGCTTGAACAATCGTTGTTCAGTTCAAGCTACCCTTGGTTTGATGCTCACTGTTTTTTTGCTTGTACCGTTTGTATTTTATTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syntaxin-6_N

Protein Analysis

344

Amino Acids

38.99

Weight (kDa)

5.98

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STX6_10_61_N PF09177 13 - 105 4.4e-19 Syntaxin 6/10/61, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 42
AccB7I CCANNNNNTGG 1 cut(s) 710
AciI CCGC 1 cut(s) 734
AclWI GGATC 2 cut(s) 590, 603
AcsI RAATTY 2 cut(s) 300, 601
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 4 cut(s) 98, 184, 369, 1008
AfiI CCNNNNNNNGG 3 cut(s) 363, 364, 710
AgsI TTSAA 7 cut(s) 293, 314, 746, 868, 949, 968, 1026
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 2 cut(s) 590, 603
ApeKI GCWGC 7 cut(s) 53, 166, 260, 401, 477, 530, 696
ApoI RAATTY 2 cut(s) 300, 601
AspS9I GGNCC 2 cut(s) 64, 464
AsuC2I CCSGG 1 cut(s) 768
AsuHPI GGTGA 3 cut(s) 257, 693, 725
AsuNHI GCTAGC 1 cut(s) 130
AvaII GGWCC 2 cut(s) 64, 464
BamHI GGATCC 1 cut(s) 595
BbsI GAAGAC 2 cut(s) 34, 802
BbvCI CCTCAGC 2 cut(s) 57, 719
BbvI GCAGC 7 cut(s) 65, 153, 272, 388, 489, 517, 708
BccI CCATC 1 cut(s) 357
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 1 cut(s) 768
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 5 cut(s) 131, 264, 455, 461, 525
BfmI CTRYAG 2 cut(s) 51, 157
BisI GCNGC 7 cut(s) 54, 167, 261, 402, 478, 531, 697
BlsI GCNGC 7 cut(s) 55, 168, 262, 403, 479, 532, 698
Bme1390I CCNGG 1 cut(s) 768
Bme18I GGWCC 2 cut(s) 64, 464
BmgT120I GGNCC 2 cut(s) 64, 464
BmiI GGNNCC 1 cut(s) 597
BmrFI CCNGG 1 cut(s) 768
BmsI GCATC 3 cut(s) 847, 902, 975
BmtI GCTAGC 1 cut(s) 134
BpiI GAAGAC 2 cut(s) 34, 802
BpmI CTGGAG 2 cut(s) 835, 870
Bpu10I CCTNAGC 2 cut(s) 57, 719
BpuMI CCSGG 1 cut(s) 768
BsaAI YACGTR 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 591, 917, 976
Bsc4I CCNNNNNNNGG 3 cut(s) 363, 364, 710
Bse1I ACTGG 1 cut(s) 853
Bse3DI GCAATG 2 cut(s) 544, 932
BseDI CCNNGG 3 cut(s) 591, 917, 976
BseGI GGATG 5 cut(s) 154, 382, 419, 652, 742
BseLI CCNNNNNNNGG 3 cut(s) 363, 364, 710
BseMI GCAATG 2 cut(s) 544, 932
BseMII CTCAG 3 cut(s) 48, 710, 911
BseNI ACTGG 1 cut(s) 853
BseRI GAGGAG 2 cut(s) 74, 133
BseXI GCAGC 7 cut(s) 65, 153, 272, 388, 489, 517, 708
BsiSI CCGG 1 cut(s) 767
BslFI GGGAC 1 cut(s) 430
BslI CCNNNNNNNGG 3 cut(s) 363, 364, 710
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 1 cut(s) 430
Bsp143I GATC 4 cut(s) 22, 250, 595, 797
BspACI CCGC 1 cut(s) 734
BspCNI CTCAG 3 cut(s) 49, 711, 910
BspLI GGNNCC 1 cut(s) 597
BspMAI CTGCAG 1 cut(s) 55
BspOI GCTAGC 1 cut(s) 134
BspPI GGATC 2 cut(s) 590, 603
BsrDI GCAATG 2 cut(s) 544, 932
BsrI ACTGG 1 cut(s) 853
BssECI CCNNGG 3 cut(s) 591, 917, 976
BssMI GATC 4 cut(s) 22, 250, 595, 797
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 2 cut(s) 591, 976
Bst4CI ACNGT 2 cut(s) 994, 1011
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 39
BstBAI YACGTR 1 cut(s) 96
BstC8I GCNNGC 4 cut(s) 13, 132, 535, 694
BstDEI CTNAG 4 cut(s) 57, 323, 719, 897
BstDSI CCRYGG 1 cut(s) 917
BstF5I GGATG 5 cut(s) 154, 382, 419, 652, 742
BstKTI GATC 4 cut(s) 25, 253, 598, 800
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 4 cut(s) 22, 250, 595, 797
BstNSI RCATGY 2 cut(s) 537, 696
BstSCI CCNGG 1 cut(s) 766
BstSFI CTRYAG 2 cut(s) 51, 157
BstSNI TACGTA 1 cut(s) 96
BstV1I GCAGC 7 cut(s) 65, 153, 272, 388, 489, 517, 708
BstV2I GAAGAC 2 cut(s) 34, 802
BstX2I RGATCY 1 cut(s) 595
BstXI CCANNNNNNTGG 1 cut(s) 878
BstYI RGATCY 1 cut(s) 595
BtgI CCRYGG 1 cut(s) 917
BtsCI GGATG 5 cut(s) 154, 382, 419, 652, 742
BtsIMutI CAGTG 1 cut(s) 990
Cac8I GCNNGC 4 cut(s) 13, 132, 535, 694
Cfr13I GGNCC 2 cut(s) 64, 464
Csp6I GTAC 4 cut(s) 97, 183, 368, 1007
CspCI CAANNNNNGTGG 2 cut(s) 927, 962
CviAII CATG 6 cut(s) 242, 269, 389, 534, 610, 693
CviQI GTAC 4 cut(s) 97, 183, 368, 1007
DdeI CTNAG 4 cut(s) 57, 323, 719, 897
DpnI GATC 4 cut(s) 24, 252, 597, 799
DpnII GATC 4 cut(s) 22, 250, 595, 797
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco105I TACGTA 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 591, 976
Eco47I GGWCC 2 cut(s) 64, 464
EcoO109I RGGNCCY 1 cut(s) 464
EcoT14I CCWWGG 2 cut(s) 591, 976
ErhI CCWWGG 2 cut(s) 591, 976
FaeI CATG 6 cut(s) 245, 272, 392, 537, 613, 696
FalI AAGNNNNNCTT 4 cut(s) 26, 58, 960, 992
FaqI GGGAC 1 cut(s) 430
FatI CATG 6 cut(s) 241, 268, 388, 533, 609, 692
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 7 cut(s) 54, 167, 261, 402, 478, 531, 697
FokI GGATG 5 cut(s) 161, 389, 406, 659, 749
Fsp4HI GCNGC 7 cut(s) 54, 167, 261, 402, 478, 531, 697
FspBI CTAG 5 cut(s) 131, 264, 455, 461, 525
GluI GCNGC 7 cut(s) 54, 167, 261, 402, 478, 531, 697
GsuI CTGGAG 2 cut(s) 835, 870
HapII CCGG 1 cut(s) 767
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 6 cut(s) 245, 272, 392, 537, 613, 696
HinfI GANTC 4 cut(s) 71, 89, 329, 614
HpaII CCGG 1 cut(s) 767
HphI GGTGA 3 cut(s) 257, 693, 725
Hpy166II GTNNAC 1 cut(s) 917
Hpy188I TCNGA 3 cut(s) 224, 553, 900
Hpy188III TCNNGA 2 cut(s) 455, 814
Hpy8I GTNNAC 1 cut(s) 917
HpyAV CCTTC 4 cut(s) 208, 338, 368, 682
HpyCH4III ACNGT 2 cut(s) 994, 1011
HpyCH4IV ACGT 2 cut(s) 39, 95
HpyF3I CTNAG 4 cut(s) 57, 323, 719, 897
HpySE526I ACGT 2 cut(s) 39, 95
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 6 cut(s) 245, 272, 392, 537, 613, 696
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 4 cut(s) 22, 250, 595, 797
LmnI GCTCC 3 cut(s) 163, 474, 851
Lsp1109I GCAGC 7 cut(s) 65, 153, 272, 388, 489, 517, 708
LweI GCATC 3 cut(s) 847, 902, 975
MaeI CTAG 5 cut(s) 131, 264, 455, 461, 525
MaeII ACGT 2 cut(s) 39, 95
MaeIII GTNAC 1 cut(s) 491
MalI GATC 4 cut(s) 24, 252, 597, 799
MboI GATC 4 cut(s) 22, 250, 595, 797
MboII GAAGA 7 cut(s) 34, 215, 392, 395, 513, 765, 802
MfeI CAATTG 2 cut(s) 833, 905
MflI RGATCY 1 cut(s) 595
MluCI AATT 9 cut(s) 275, 300, 601, 630, 706, 762, 833, 859, 905
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 7 cut(s) 52, 55, 111, 636, 714, 769, 810
MseI TTAA 4 cut(s) 519, 570, 858, 1030
MslI CAYNNNNRTG 2 cut(s) 147, 246
MspA1I CMGCKG 3 cut(s) 56, 77, 373
MspI CCGG 1 cut(s) 767
MspR9I CCNGG 1 cut(s) 768
MunI CAATTG 2 cut(s) 833, 905
NciI CCSGG 1 cut(s) 768
NdeII GATC 4 cut(s) 22, 250, 595, 797
NheI GCTAGC 1 cut(s) 130
NlaIII CATG 6 cut(s) 245, 272, 392, 537, 613, 696
NlaIV GGNNCC 1 cut(s) 597
NmuCI GTSAC 1 cut(s) 491
NspI RCATGY 2 cut(s) 537, 696
PaeI GCATGC 2 cut(s) 537, 696
PfeI GAWTC 4 cut(s) 71, 89, 329, 614
PflMI CCANNNNNTGG 1 cut(s) 710
PfoI TCCNGGA 1 cut(s) 766
PkrI GCNGC 7 cut(s) 55, 168, 262, 403, 479, 532, 698
Ppu21I YACGTR 1 cut(s) 96
PpuMI RGGWCCY 1 cut(s) 464
Psp5II RGGWCCY 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 597
PspPI GGNCC 2 cut(s) 64, 464
PspPPI RGGWCCY 1 cut(s) 464
PstI CTGCAG 1 cut(s) 55
PsuI RGATCY 1 cut(s) 595
PvuII CAGCTG 3 cut(s) 56, 77, 373
RsaI GTAC 4 cut(s) 98, 184, 369, 1008
RsaNI GTAC 4 cut(s) 97, 183, 368, 1007
RseI CAYNNNNRTG 2 cut(s) 147, 246
SaqAI TTAA 4 cut(s) 519, 570, 858, 1030
SatI GCNGC 7 cut(s) 54, 167, 261, 402, 478, 531, 697
Sau3AI GATC 4 cut(s) 22, 250, 595, 797
Sau96I GGNCC 2 cut(s) 64, 464
ScrFI CCNGG 1 cut(s) 768
SfaNI GCATC 3 cut(s) 847, 902, 975
SfcI CTRYAG 2 cut(s) 51, 157
SinI GGWCC 2 cut(s) 64, 464
SmiMI CAYNNNNRTG 2 cut(s) 147, 246
SnaBI TACGTA 1 cut(s) 96
SphI GCATGC 2 cut(s) 537, 696
Sse9I AATT 9 cut(s) 275, 300, 601, 630, 706, 762, 833, 859, 905
SsiI CCGC 1 cut(s) 734
SspMI CTAG 5 cut(s) 131, 264, 455, 461, 525
StyD4I CCNGG 1 cut(s) 766
StyI CCWWGG 2 cut(s) 591, 976
TaaI ACNGT 2 cut(s) 994, 1011
TaiI ACGT 2 cut(s) 42, 98
TaqI TCGA 3 cut(s) 112, 361, 617
TasI AATT 9 cut(s) 275, 300, 601, 630, 706, 762, 833, 859, 905
TfiI GAWTC 4 cut(s) 71, 89, 329, 614
Tru1I TTAA 4 cut(s) 519, 570, 858, 1030
Tru9I TTAA 4 cut(s) 519, 570, 858, 1030
TscAI CASTG 1 cut(s) 997
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 7 cut(s) 53, 166, 260, 401, 477, 530, 696
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 6 cut(s) 165, 393, 462, 527, 570, 753
TspGWI ACGGA 1 cut(s) 934
TspRI CASTG 1 cut(s) 997
Van91I CCANNNNNTGG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 64, 464
XapI RAATTY 2 cut(s) 300, 601
XbaI TCTAGA 1 cut(s) 454
XceI RCATGY 2 cut(s) 537, 696
XcmI CCANNNNNNNNNTGG 2 cut(s) 196, 924
XspI CTAG 5 cut(s) 131, 264, 455, 461, 525
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.