pycom13g23560

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007465
Physical Location & Seq
Forward (+)
21058132 .. 21062487
4356 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1494 bp
ATGTTGGTTAACAAGGTGAGCAAAGCTCCCAATCTTTTCACCATCGGAAACTTTAGCGTATTTTCTTGTGCAACACCTAGTTCAACATATCCAATCCTTGAGACACCTAATACGAGTACTTGGCCGCCCTATCGGATAATTATAAAGTTCTTCATTCGCCTACTCAACTTTTCACCAGTTGGCAGCATGTCATTTCGTTGGATAGCAACCTCTGCACACACTTCAATGGAAAAGATGACACCGTTCATGACGACCACCACGACGGATCCGCCATCCAAATTGTATGAAGTCCGATCCTTCACCCCGGTCGTTTTACCATTCTTCTTTGTTCCGATAGGGTTTGAGAGGTGGGAGGGGAGATCGACGCGTCATACTTTTTTCTTGAAACGGGCCTCCATATGCTGCTTGCGTATTTCTTCATTAAATGAAAATTTTCACAGACAAGAAAACAAAATGAAAAACGCAAAAGGGACAGACACAAATAATAGAAAACCGCCAAAAAAAATCCGGGTATCTCCCCGGGGCGCCTACATTCATGATACATTCATTGGACAGCAGCTTCCGTTTCCCTGCTCATCTCCAAATCTGTCGTACCTTTACTCAAACACTTTATCAAAATCCAAATCTCTCTCTCTCTCTCCCCTAAAACACATGATCGCCGCTAATTCTCCGCTCAATTCCGCAATCCTCATCCTCCGCACCGCCGACCCGACCCGAAAGCACTGCTCTTTCGAGCCCCTGTGTTCCAGCAACCACCTAAGTTTTAGCTCCTCCGAGCGTGGGTTGTGGATCACTCGGACCCGGATGGACTTGCTCCGCCGATTTGTTACCGGAGGATGCGTCCGTGACCCCGATCACCAGGGGATAAAGGTGGAGATCGATAGCGGCGGCAAAGATGTATTTGCCGCCGACCCCAAGGGCAGCGGCGTGCCGGAACATCTAGTCATAATGGTCAATGGAATCATCGGCAGTGCTTCAGACTGGAGATACGCCGTAGATCAGTTTGTGAAAAAGCTTCCTGATAAAGTAATTGTGCACCGTAGTGAATGTAACTCTTCAAGACTGACATTTGATGGTGTTGACTTGATGGGTGAGAGGCTAGCTGAAGAGGTATTAGCTGTCGTCAGAAAGAGGTCTGAGGTGCGGAAAATATCTTTTGTGGCTCATTCCTTAGGGGGGTTGGTTGCAAGGTATGCCATTGGAAGGCTGTACAAACCTTTTCCAAAATCAGAACCCTCGGGTCAAAATGGTAGTTGTGTAATTGAAGGGCATACAAATAAGTTGACACAATCCATTGACCAGCCTCACCATGGTACAATTGCTGGACTGGAAGCAATGAACTTTATAACAGTCGCAACACCACATCTTGGTTCAAGGGGAAATAAACAGCTCCCGTTTCTCTGTGGTCTTCCTTTTCTCGAGAGAAGAGTTTCTCAAACTGCACACTTTATTGTTGGGAGATCCTGGGAAGCATCTCTTCCTAACAGACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

498

Amino Acids

55.56

Weight (kDa)

9.92

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 311 - 488 4.5e-47 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 143, 1346
AccB1I GGYRCC 1 cut(s) 524
AccB7I CCANNNNNTGG 1 cut(s) 1367
AccBSI CCGCTC 1 cut(s) 673
AccII CGCG 1 cut(s) 367
AclWI GGATC 5 cut(s) 260, 273, 288, 797, 1455
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 2 cut(s) 960, 1125
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 4 cut(s) 118, 593, 1211, 1315
AfiI CCNNNNNNNGG 5 cut(s) 780, 1176, 1203, 1310, 1367
AflIII ACRYGT 1 cut(s) 365
AgsI TTSAA 6 cut(s) 84, 225, 385, 1059, 1265, 1374
AjnI CCWGG 2 cut(s) 858, 1463
AleI CACNNNNGTG 1 cut(s) 1041
AluBI AGCT 7 cut(s) 26, 559, 768, 1015, 1103, 1118, 1390
AluI AGCT 7 cut(s) 26, 559, 768, 1015, 1103, 1118, 1390
Alw21I GWGCWC 1 cut(s) 1038
Alw26I GTCTC 1 cut(s) 95
Alw44I GTGCAC 1 cut(s) 1034
AlwI GGATC 5 cut(s) 260, 273, 288, 797, 1455
Ama87I CYCGRG 3 cut(s) 519, 1237, 1418
AoxI GGCC 2 cut(s) 122, 390
ApaLI GTGCAC 1 cut(s) 1034
ApeKI GCWGC 4 cut(s) 183, 402, 556, 921
ApoI RAATTY 1 cut(s) 430
Asp700I GAANNNNTTC 2 cut(s) 432, 1429
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 2 cut(s) 390, 798
AsuC2I CCSGG 5 cut(s) 305, 509, 520, 521, 802
AsuHPI GGTGA 7 cut(s) 28, 31, 165, 292, 848, 1103, 1298
AsuNHI GCTAGC 1 cut(s) 1099
AvaI CYCGRG 3 cut(s) 519, 1237, 1418
AvaII GGWCC 1 cut(s) 798
AxyI CCTNAGG 1 cut(s) 1171
BaeGI GKGCMC 1 cut(s) 1038
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 524
BanII GRGCYC 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 1400
Bbv12I GWGCWC 1 cut(s) 1038
BbvI GCAGC 4 cut(s) 195, 389, 568, 933
BccI CCATC 5 cut(s) 50, 280, 799, 1067, 1081
BceAI ACGGC 1 cut(s) 977
BcgI CGANNNNNNTGC 2 cut(s) 705, 739
BciT130I CCWGG 2 cut(s) 860, 1465
BcnI CCSGG 5 cut(s) 305, 509, 520, 521, 802
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 3 cut(s) 78, 941, 1100
BfoI RGCGCY 1 cut(s) 528
BmcAI AGTACT 1 cut(s) 118
Bme1390I CCNGG 7 cut(s) 305, 509, 520, 521, 802, 860, 1465
Bme18I GGWCC 1 cut(s) 798
BmeT110I CYCGRG 3 cut(s) 519, 1237, 1418
BmgT120I GGNCC 2 cut(s) 390, 798
BmiI GGNNCC 3 cut(s) 267, 526, 800
BmrFI CCNGG 7 cut(s) 305, 509, 520, 521, 802, 860, 1465
BmsI GCATC 2 cut(s) 827, 1481
BmtI GCTAGC 1 cut(s) 1103
BpiI GAAGAC 1 cut(s) 1400
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 1 cut(s) 1003
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 5 cut(s) 305, 509, 520, 521, 802
Bsa29I ATCGAT 1 cut(s) 879
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 9 cut(s) 303, 518, 519, 520, 859, 915, 1236, 1309, 1464
BsaWI WCCGGW 1 cut(s) 830
BsaXI ACNNNNNCTCC 2 cut(s) 825, 855
Bsc4I CCNNNNNNNGG 5 cut(s) 780, 1176, 1203, 1310, 1367
Bse1I ACTGG 3 cut(s) 176, 986, 1332
Bse21I CCTNAGG 1 cut(s) 1171
Bse3DI GCAATG 1 cut(s) 1341
BseBI CCWGG 2 cut(s) 860, 1465
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 9 cut(s) 303, 518, 519, 520, 859, 915, 1236, 1309, 1464
BseGI GGATG 4 cut(s) 272, 690, 810, 842
BseLI CCNNNNNNNGG 5 cut(s) 780, 1176, 1203, 1310, 1367
BseMI GCAATG 1 cut(s) 1341
BseMII CTCAG 1 cut(s) 1128
BseNI ACTGG 3 cut(s) 176, 986, 1332
BseRI GAGGAG 1 cut(s) 760
BseSI GKGCMC 1 cut(s) 1038
BseXI GCAGC 4 cut(s) 195, 389, 568, 933
BsgI GTGCAG 2 cut(s) 198, 1425
Bsh1236I CGCG 1 cut(s) 367
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 2 cut(s) 124, 392
BshNI GGYRCC 1 cut(s) 524
BshVI ATCGAT 1 cut(s) 879
BsiEI CGRYCG 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 1038
BsiHKCI CYCGRG 3 cut(s) 519, 1237, 1418
BsiSI CCGG 6 cut(s) 305, 508, 520, 802, 831, 932
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 5 cut(s) 780, 1176, 1203, 1310, 1367
BsmAI GTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 484
BsnI GGCC 2 cut(s) 124, 392
BsoBI CYCGRG 3 cut(s) 519, 1237, 1418
Bsp1286I GDGCHC 2 cut(s) 738, 1038
Bsp1407I TGTACA 1 cut(s) 1209
Bsp143I GATC 9 cut(s) 265, 293, 359, 654, 789, 853, 876, 997, 1460
Bsp19I CCATGG 1 cut(s) 1309
BspANI GGCC 2 cut(s) 124, 392
BspCNI CTCAG 1 cut(s) 1129
BspDI ATCGAT 1 cut(s) 879
BspFNI CGCG 1 cut(s) 367
BspHI TCATGA 2 cut(s) 246, 535
BspLI GGNNCC 3 cut(s) 267, 526, 800
BspOI GCTAGC 1 cut(s) 1103
BspPI GGATC 5 cut(s) 260, 273, 288, 797, 1455
BspT107I GGYRCC 1 cut(s) 524
BsrBI CCGCTC 1 cut(s) 673
BsrDI GCAATG 1 cut(s) 1341
BsrGI TGTACA 1 cut(s) 1209
BsrI ACTGG 3 cut(s) 176, 986, 1332
BssECI CCNNGG 9 cut(s) 303, 518, 519, 520, 859, 915, 1236, 1309, 1464
BssMI GATC 9 cut(s) 265, 293, 359, 654, 789, 853, 876, 997, 1460
BssNI GRCGYC 1 cut(s) 525
BssT1I CCWWGG 2 cut(s) 915, 1309
Bst2UI CCWGG 2 cut(s) 860, 1465
Bst4CI ACNGT 3 cut(s) 243, 1040, 1351
Bst6I CTCTTC 4 cut(s) 1060, 1101, 1420, 1482
BstACI GRCGYC 1 cut(s) 525
BstAPI GCANNNNNTGC 2 cut(s) 212, 1193
BstAUI TGTACA 1 cut(s) 1209
BstC8I GCNNGC 3 cut(s) 407, 929, 1101
BstDEI CTNAG 3 cut(s) 758, 1137, 1171
BstDSI CCRYGG 1 cut(s) 1309
BstF5I GGATG 4 cut(s) 272, 690, 810, 842
BstFNI CGCG 1 cut(s) 367
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 9 cut(s) 268, 296, 362, 657, 792, 856, 879, 1000, 1463
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 9 cut(s) 265, 293, 359, 654, 789, 853, 876, 997, 1460
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 2 cut(s) 212, 1193
BstNI CCWGG 2 cut(s) 860, 1465
BstNSI RCATGY 1 cut(s) 190
BstSCI CCNGG 7 cut(s) 303, 507, 518, 519, 800, 858, 1463
BstSLI GKGCMC 1 cut(s) 1038
BstUI CGCG 1 cut(s) 367
BstV1I GCAGC 4 cut(s) 195, 389, 568, 933
BstV2I GAAGAC 1 cut(s) 1400
BstX2I RGATCY 2 cut(s) 265, 1460
BstYI RGATCY 2 cut(s) 265, 1460
Bsu15I ATCGAT 1 cut(s) 879
Bsu36I CCTNAGG 1 cut(s) 1171
BsuRI GGCC 2 cut(s) 124, 392
BsuTUI ATCGAT 1 cut(s) 879
BtgI CCRYGG 1 cut(s) 1309
BtsCI GGATG 4 cut(s) 272, 690, 810, 842
BtsI GCAGTG 2 cut(s) 721, 976
BtsIMutI CAGTG 2 cut(s) 721, 976
Cac8I GCNNGC 3 cut(s) 407, 929, 1101
CciI TCATGA 2 cut(s) 246, 535
CfoI GCGC 1 cut(s) 527
Cfr13I GGNCC 2 cut(s) 390, 798
Cfr9I CCCGGG 1 cut(s) 519
ClaI ATCGAT 1 cut(s) 879
CseI GACGC 3 cut(s) 356, 373, 829
Csp6I GTAC 4 cut(s) 117, 592, 1210, 1314
CviAII CATG 5 cut(s) 187, 247, 536, 652, 1310
CviQI GTAC 4 cut(s) 117, 592, 1210, 1314
DdeI CTNAG 3 cut(s) 758, 1137, 1171
DinI GGCGCC 1 cut(s) 526
DpnI GATC 9 cut(s) 267, 295, 361, 656, 791, 855, 878, 999, 1462
DpnII GATC 9 cut(s) 265, 293, 359, 654, 789, 853, 876, 997, 1460
EaeI YGGCCR 1 cut(s) 122
Eam1104I CTCTTC 4 cut(s) 1060, 1101, 1420, 1482
EarI CTCTTC 4 cut(s) 1060, 1101, 1420, 1482
EciI GGCGGA 2 cut(s) 258, 806
Eco130I CCWWGG 2 cut(s) 915, 1309
Eco24I GRGCYC 1 cut(s) 738
Eco47I GGWCC 1 cut(s) 798
Eco57I CTGAAG 2 cut(s) 960, 1125
Eco81I CCTNAGG 1 cut(s) 1171
Eco88I CYCGRG 3 cut(s) 519, 1237, 1418
EcoRII CCWGG 2 cut(s) 858, 1463
EcoT14I CCWWGG 2 cut(s) 915, 1309
EcoT38I GRGCYC 1 cut(s) 738
EgeI GGCGCC 1 cut(s) 526
EheI GGCGCC 1 cut(s) 526
ErhI CCWWGG 2 cut(s) 915, 1309
FaeI CATG 5 cut(s) 190, 250, 539, 655, 1313
FalI AAGNNNNNCTT 2 cut(s) 1461, 1493
FaqI GGGAC 1 cut(s) 484
FatI CATG 5 cut(s) 186, 246, 535, 651, 1309
FauNDI CATATG 1 cut(s) 398
FokI GGATG 4 cut(s) 259, 677, 817, 849
FriOI GRGCYC 1 cut(s) 738
FspBI CTAG 3 cut(s) 78, 941, 1100
GlaI GCGC 1 cut(s) 526
GsuI CTGGAG 1 cut(s) 1003
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 2 cut(s) 124, 392
HapII CCGG 6 cut(s) 305, 508, 520, 802, 831, 932
HgaI GACGC 3 cut(s) 356, 373, 829
HhaI GCGC 1 cut(s) 527
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 5 cut(s) 190, 250, 539, 655, 1313
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HincII GTYRAC 3 cut(s) 10, 1081, 1284
HindII GTYRAC 3 cut(s) 10, 1081, 1284
HindIII AAGCTT 1 cut(s) 1013
HinfI GANTC 1 cut(s) 960
HpaI GTTAAC 1 cut(s) 10
HpaII CCGG 6 cut(s) 305, 508, 520, 802, 831, 932
HphI GGTGA 7 cut(s) 28, 31, 165, 292, 848, 1103, 1298
Hpy166II GTNNAC 4 cut(s) 10, 1036, 1081, 1284
Hpy188III TCNNGA 7 cut(s) 247, 382, 536, 1019, 1059, 1418, 1420
Hpy8I GTNNAC 4 cut(s) 10, 1036, 1081, 1284
Hpy99I CGWCG 2 cut(s) 265, 367
HpyAV CCTTC 3 cut(s) 307, 1197, 1259
HpyCH4III ACNGT 3 cut(s) 243, 1040, 1351
HpyCH4V TGCA 5 cut(s) 71, 215, 1036, 1187, 1442
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 1193
HpyF3I CTNAG 3 cut(s) 758, 1137, 1171
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 5 cut(s) 190, 250, 539, 655, 1313
HspAI GCGC 1 cut(s) 525
KasI GGCGCC 1 cut(s) 524
KspAI GTTAAC 1 cut(s) 10
Kzo9I GATC 9 cut(s) 265, 293, 359, 654, 789, 853, 876, 997, 1460
LmnI GCTCC 4 cut(s) 31, 773, 819, 1395
Lsp1109I GCAGC 4 cut(s) 195, 389, 568, 933
LweI GCATC 2 cut(s) 827, 1481
MaeI CTAG 3 cut(s) 78, 941, 1100
MaeIII GTNAC 3 cut(s) 826, 845, 1049
MalI GATC 9 cut(s) 267, 295, 361, 656, 791, 855, 878, 999, 1462
MbiI CCGCTC 1 cut(s) 673
MboI GATC 9 cut(s) 265, 293, 359, 654, 789, 853, 876, 997, 1460
MboII GAAGA 8 cut(s) 142, 313, 408, 1047, 1118, 1400, 1437, 1469
MfeI CAATTG 1 cut(s) 1317
MflI RGATCY 2 cut(s) 265, 1460
MhlI GDGCHC 2 cut(s) 738, 1038
MluCI AATT 8 cut(s) 138, 278, 430, 664, 676, 1029, 1260, 1317
MluI ACGCGT 1 cut(s) 365
Mly113I GGCGCC 1 cut(s) 525
MmeI TCCRAC 1 cut(s) 179
MroXI GAANNNNTTC 2 cut(s) 432, 1429
MseI TTAA 2 cut(s) 9, 422
MslI CAYNNNNRTG 2 cut(s) 224, 1041
MspA1I CMGCKG 1 cut(s) 924
MspI CCGG 6 cut(s) 305, 508, 520, 802, 831, 932
MspR9I CCNGG 7 cut(s) 305, 509, 520, 521, 802, 860, 1465
MunI CAATTG 1 cut(s) 1317
MvaI CCWGG 2 cut(s) 860, 1465
MvnI CGCG 1 cut(s) 367
MwoI GCNNNNNNNGC 2 cut(s) 212, 1193
NarI GGCGCC 1 cut(s) 525
NciI CCSGG 5 cut(s) 305, 509, 520, 521, 802
NcoI CCATGG 1 cut(s) 1309
NdeI CATATG 1 cut(s) 398
NdeII GATC 9 cut(s) 265, 293, 359, 654, 789, 853, 876, 997, 1460
NheI GCTAGC 1 cut(s) 1099
NlaIII CATG 5 cut(s) 190, 250, 539, 655, 1313
NlaIV GGNNCC 3 cut(s) 267, 526, 800
NmuCI GTSAC 1 cut(s) 845
NspI RCATGY 1 cut(s) 190
OliI CACNNNNGTG 1 cut(s) 1041
PaeR7I CTCGAG 1 cut(s) 1418
PagI TCATGA 2 cut(s) 246, 535
PcsI WCGNNNNNNNCGW 1 cut(s) 257
PdmI GAANNNNTTC 2 cut(s) 432, 1429
PfeI GAWTC 1 cut(s) 960
PflMI CCANNNNNTGG 1 cut(s) 1367
PluTI GGCGCC 1 cut(s) 528
PsiI TTATAA 2 cut(s) 143, 1346
Psp6I CCWGG 2 cut(s) 858, 1463
PspGI CCWGG 2 cut(s) 858, 1463
PspN4I GGNNCC 3 cut(s) 267, 526, 800
PspPI GGNCC 2 cut(s) 390, 798
PsuI RGATCY 2 cut(s) 265, 1460
RsaI GTAC 4 cut(s) 118, 593, 1211, 1315
RsaNI GTAC 4 cut(s) 117, 592, 1210, 1314
RseI CAYNNNNRTG 2 cut(s) 224, 1041
SaqAI TTAA 2 cut(s) 9, 422
Sau3AI GATC 9 cut(s) 265, 293, 359, 654, 789, 853, 876, 997, 1460
Sau96I GGNCC 2 cut(s) 390, 798
ScaI AGTACT 1 cut(s) 118
ScrFI CCNGG 7 cut(s) 305, 509, 520, 521, 802, 860, 1465
SduI GDGCHC 2 cut(s) 738, 1038
SfaNI GCATC 2 cut(s) 827, 1481
SfoI GGCGCC 1 cut(s) 526
Sfr274I CTCGAG 1 cut(s) 1418
SinI GGWCC 1 cut(s) 798
SlaI CTCGAG 1 cut(s) 1418
SmaI CCCGGG 1 cut(s) 521
SmiMI CAYNNNNRTG 2 cut(s) 224, 1041
SmlI CTYRAG 2 cut(s) 98, 1418
SmoI CTYRAG 2 cut(s) 98, 1418
Sse9I AATT 8 cut(s) 138, 278, 430, 664, 676, 1029, 1260, 1317
SspDI GGCGCC 1 cut(s) 524
SspMI CTAG 3 cut(s) 78, 941, 1100
StyD4I CCNGG 7 cut(s) 303, 507, 518, 519, 800, 858, 1463
StyI CCWWGG 2 cut(s) 915, 1309
TaaI ACNGT 3 cut(s) 243, 1040, 1351
TaqI TCGA 4 cut(s) 362, 732, 879, 1419
TasI AATT 8 cut(s) 138, 278, 430, 664, 676, 1029, 1260, 1317
TatI WGTACW 2 cut(s) 116, 1209
TauI GCSGC 6 cut(s) 127, 662, 888, 891, 908, 927
TfiI GAWTC 1 cut(s) 960
Tru1I TTAA 2 cut(s) 9, 422
Tru9I TTAA 2 cut(s) 9, 422
TscAI CASTG 2 cut(s) 728, 976
TseFI GTSAC 1 cut(s) 845
TseI GCWGC 4 cut(s) 183, 402, 556, 921
Tsp45I GTSAC 1 cut(s) 845
TspDTI ATGAA 9 cut(s) 142, 235, 300, 408, 441, 470, 524, 535, 1352
TspGWI ACGGA 3 cut(s) 278, 552, 833
TspMI CCCGGG 1 cut(s) 519
TspRI CASTG 2 cut(s) 728, 976
Van91I CCANNNNNTGG 1 cut(s) 1367
VneI GTGCAC 1 cut(s) 1034
VpaK11BI GGWCC 1 cut(s) 798
XapI RAATTY 1 cut(s) 430
XceI RCATGY 1 cut(s) 190
XcmI CCANNNNNNNNNTGG 1 cut(s) 1307
XhoI CTCGAG 1 cut(s) 1418
XmaI CCCGGG 1 cut(s) 519
XmnI GAANNNNTTC 2 cut(s) 432, 1429
XspI CTAG 3 cut(s) 78, 941, 1100
ZrmI AGTACT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.