pycom14g00150

5-adenylylsulfate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008614
Physical Location & Seq
Forward (+)
81614 .. 85212
3599 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 900 bp
ATGGATAAATCTGTAATCTTTTTGTGTATCGCTGCCTTGGCAACTTCGCTACGATTAGTTTCTTCGTCTTCTGTTTTATGCCCTCACGACTCCGATTTCTTACGGTACAGCCTTCAATCTCAATGCCCCATCTGGATCACCCCCCATCCACCCCTCAAGGTGGATGGAAACTTCCTAGACAGAGCTTTGGCATCGCAACAGAGGACTGATTATACAGCAGTGCTCTTTTATGCTTCCTGGTGCCCATTTTCACGCACAATGTATCCTACATTTGAAAAGCTCAGCTTCATGTTCCCTCAAGTAGAACATTTGGCCATTGAGCAATCTTCAGCCCTGCCGAGTGTGTTCTCAAGATATGGAATACATAGTTTCCCTTCGATTTTAATAGTCAATCAGACATCAAGGGTGCGTTATCATGGTCCAAAACGTCTCTCCTCGCTTGCACAGTTTTACCAGAAAACCACAGGACTTAAACCAGTTCAATATTTTGATGGCGATCAAATAGTCAGCTTAAACATTCGTGAAAAATCTTTTATCCAGTCAATGGGTAACATGTCACTCAGGGAGATATCAAGGAGGGAACCCTATTTAGCTTTTGCCATATTGTTTCTCTGTTTGAGGGTGCTTCTGTATATATTCCCAAAGGTATTGACTCGCCTCCATGCTTTCTGGGTCCTATACGTTCCCCATTTTAACTTGGGAATATTTGGGGAGACGAGTCAAAGTATGGGGCGTATCCTCCCCATGGTTGACGTTAGGAGGATTTGGACCAAGCTGAGATTATGCAAGACGAGGAACTTCCACGAGGGAGCAAAGAATGCCAGGGTTTGGGCTTCTTCCCTAACTTCTGTCTCCTTGGGCAAATCTTCATCTGCTCGATCGTCGTCGTTGTCATCTTAA

Protein Analysis

300

Amino Acids

34.09

Weight (kDa)

9.92

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 69 - 152 2.4e-08 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 240
AccB7I CCANNNNNTGG 2 cut(s) 544, 828
AclWI GGATC 1 cut(s) 143
AcoI YGGCCR 1 cut(s) 312
AcuI CTGAAG 1 cut(s) 312
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 4 cut(s) 160, 544, 745, 828
AflIII ACRYGT 1 cut(s) 552
AgsI TTSAA 3 cut(s) 116, 275, 482
AjnI CCWGG 2 cut(s) 236, 821
AluBI AGCT 6 cut(s) 185, 280, 285, 510, 593, 775
AluI AGCT 6 cut(s) 185, 280, 285, 510, 593, 775
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 3 cut(s) 434, 707, 856
AlwI GGATC 1 cut(s) 143
AoxI GGCC 1 cut(s) 312
ApeKI GCWGC 1 cut(s) 32
AspS9I GGNCC 3 cut(s) 419, 673, 768
AsuHPI GGTGA 1 cut(s) 130
AvaII GGWCC 3 cut(s) 419, 673, 768
BaeGI GKGCMC 1 cut(s) 245
BalI TGGCCA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 240
BauI CACGAG 1 cut(s) 803
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 1 cut(s) 19
BccI CCATC 4 cut(s) 137, 153, 158, 485
BciT130I CCWGG 2 cut(s) 238, 823
BciVI GTATCC 2 cut(s) 273, 746
BcoDI GTCTC 3 cut(s) 434, 707, 856
BfaI CTAG 1 cut(s) 176
BfuI GTATCC 2 cut(s) 273, 746
BisI GCNGC 1 cut(s) 33
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 2 cut(s) 238, 823
Bme18I GGWCC 3 cut(s) 419, 673, 768
BmgT120I GGNCC 3 cut(s) 419, 673, 768
BmiI GGNNCC 3 cut(s) 242, 582, 674
BmrFI CCNGG 2 cut(s) 238, 823
BmsI GCATC 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 281
BpuEI CTTGAG 3 cut(s) 140, 282, 334
BsaBI GATNNNNATC 1 cut(s) 495
BsaJI CCNNGG 4 cut(s) 36, 744, 822, 855
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 544, 745, 828
Bse1I ACTGG 2 cut(s) 476, 538
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 2 cut(s) 238, 823
BseDI CCNNGG 4 cut(s) 36, 744, 822, 855
BseGI GGATG 2 cut(s) 145, 169
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 4 cut(s) 160, 544, 745, 828
BseMII CTCAG 3 cut(s) 295, 574, 767
BseNI ACTGG 2 cut(s) 476, 538
BseRI GAGGAG 1 cut(s) 424
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 1 cut(s) 19
Bsh1285I CGRYCG 1 cut(s) 881
BshFI GGCC 1 cut(s) 314
BshNI GGYRCC 1 cut(s) 240
BsiEI CGRYCG 1 cut(s) 881
BsiHKAI GWGCWC 1 cut(s) 225
BslI CCNNNNNNNGG 4 cut(s) 160, 544, 745, 828
BsmAI GTCTC 3 cut(s) 434, 707, 856
BsmBI CGTCTC 2 cut(s) 434, 707
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 1 cut(s) 314
Bsp1286I GDGCHC 2 cut(s) 225, 245
Bsp143I GATC 3 cut(s) 135, 496, 878
Bsp1720I GCTNAGC 1 cut(s) 281
Bsp19I CCATGG 1 cut(s) 744
BspANI GGCC 1 cut(s) 314
BspCNI CTCAG 3 cut(s) 294, 573, 768
BspLI GGNNCC 3 cut(s) 242, 582, 674
BspPI GGATC 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 240
BsrI ACTGG 2 cut(s) 476, 538
BssECI CCNNGG 4 cut(s) 36, 744, 822, 855
BssMI GATC 3 cut(s) 135, 496, 878
BssSI CACGAG 1 cut(s) 803
BssT1I CCWWGG 3 cut(s) 36, 744, 855
Bst2BI CACGAG 1 cut(s) 803
Bst2UI CCWGG 2 cut(s) 238, 823
Bst4CI ACNGT 2 cut(s) 105, 447
BstAPI GCANNNNNTGC 1 cut(s) 818
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 3 cut(s) 281, 560, 776
BstDSI CCRYGG 1 cut(s) 744
BstF5I GGATG 2 cut(s) 145, 169
BstKTI GATC 3 cut(s) 138, 499, 881
BstMAI GTCTC 3 cut(s) 434, 707, 856
BstMBI GATC 3 cut(s) 135, 496, 878
BstMCI CGRYCG 1 cut(s) 881
BstMWI GCNNNNNNNGC 2 cut(s) 38, 818
BstNI CCWGG 2 cut(s) 238, 823
BstNSI RCATGY 1 cut(s) 556
BstSCI CCNGG 2 cut(s) 236, 821
BstSLI GKGCMC 1 cut(s) 245
BstV1I GCAGC 1 cut(s) 19
BstV2I GAAGAC 1 cut(s) 60
BsuI GTATCC 2 cut(s) 273, 746
BsuRI GGCC 1 cut(s) 314
BtgI CCRYGG 1 cut(s) 744
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 2 cut(s) 145, 169
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 441
Cfr13I GGNCC 3 cut(s) 419, 673, 768
Csp6I GTAC 1 cut(s) 106
CviAII CATG 5 cut(s) 289, 416, 553, 662, 745
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 3 cut(s) 281, 560, 776
DpnI GATC 3 cut(s) 137, 498, 880
DpnII GATC 3 cut(s) 135, 496, 878
EaeI YGGCCR 1 cut(s) 312
Eco130I CCWWGG 3 cut(s) 36, 744, 855
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 3 cut(s) 419, 673, 768
Eco57I CTGAAG 1 cut(s) 312
EcoO109I RGGNCCY 1 cut(s) 673
EcoRII CCWGG 2 cut(s) 236, 821
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 3 cut(s) 36, 744, 855
ErhI CCWWGG 3 cut(s) 36, 744, 855
Esp3I CGTCTC 2 cut(s) 434, 707
FaeI CATG 5 cut(s) 292, 419, 556, 665, 748
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FatI CATG 5 cut(s) 288, 415, 552, 661, 744
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 2 cut(s) 132, 176
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 1 cut(s) 176
GluI GCNGC 1 cut(s) 33
HaeIII GGCC 1 cut(s) 314
Hin1II CATG 5 cut(s) 292, 419, 556, 665, 748
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HinfI GANTC 3 cut(s) 89, 652, 718
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 751
Hpy188I TCNGA 2 cut(s) 94, 396
Hpy188III TCNNGA 4 cut(s) 86, 133, 351, 521
Hpy8I GTNNAC 1 cut(s) 751
Hpy99I CGWCG 2 cut(s) 886, 889
HpyAV CCTTC 2 cut(s) 122, 384
HpyCH4III ACNGT 2 cut(s) 105, 447
HpyCH4IV ACGT 3 cut(s) 427, 681, 753
HpyCH4V TGCA 2 cut(s) 443, 786
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 818
HpyF3I CTNAG 3 cut(s) 281, 560, 776
HpySE526I ACGT 3 cut(s) 427, 681, 753
Hsp92II CATG 5 cut(s) 292, 419, 556, 665, 748
Kzo9I GATC 3 cut(s) 135, 496, 878
LmnI GCTCC 1 cut(s) 809
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 1 cut(s) 200
MaeI CTAG 1 cut(s) 176
MaeII ACGT 3 cut(s) 427, 681, 753
MaeIII GTNAC 2 cut(s) 548, 555
MalI GATC 3 cut(s) 137, 498, 880
MboI GATC 3 cut(s) 135, 496, 878
MboII GAAGA 5 cut(s) 54, 60, 318, 828, 858
MhlI GDGCHC 2 cut(s) 225, 245
MlsI TGGCCA 1 cut(s) 314
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 3 cut(s) 83, 646, 727
Mox20I TGGCCA 1 cut(s) 314
MscI TGGCCA 1 cut(s) 314
MseI TTAA 5 cut(s) 383, 471, 512, 693, 898
Msp20I TGGCCA 1 cut(s) 314
MspR9I CCNGG 2 cut(s) 238, 823
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 2 cut(s) 238, 823
MwoI GCNNNNNNNGC 2 cut(s) 38, 818
NcoI CCATGG 1 cut(s) 744
NdeII GATC 3 cut(s) 135, 496, 878
NlaIII CATG 5 cut(s) 292, 419, 556, 665, 748
NlaIV GGNNCC 3 cut(s) 242, 582, 674
NmeAIII GCCGAG 1 cut(s) 363
NmuCI GTSAC 1 cut(s) 555
NspI RCATGY 1 cut(s) 556
PciI ACATGT 1 cut(s) 552
PctI GAATGC 1 cut(s) 823
PflMI CCANNNNNTGG 2 cut(s) 544, 828
PkrI GCNGC 1 cut(s) 34
Ple19I CGATCG 1 cut(s) 881
PleI GAGTC 3 cut(s) 83, 646, 726
PpsI GAGTC 3 cut(s) 83, 646, 726
PpuMI RGGWCCY 1 cut(s) 673
PscI ACATGT 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 673
Psp6I CCWGG 2 cut(s) 236, 821
PspGI CCWGG 2 cut(s) 236, 821
PspN4I GGNNCC 3 cut(s) 242, 582, 674
PspPI GGNCC 3 cut(s) 419, 673, 768
PspPPI RGGWCCY 1 cut(s) 673
PvuI CGATCG 1 cut(s) 881
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SaqAI TTAA 5 cut(s) 383, 471, 512, 693, 898
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 3 cut(s) 135, 496, 878
Sau96I GGNCC 3 cut(s) 419, 673, 768
SchI GAGTC 3 cut(s) 83, 646, 727
ScrFI CCNGG 2 cut(s) 238, 823
SduI GDGCHC 2 cut(s) 225, 245
SfaNI GCATC 1 cut(s) 200
SinI GGWCC 3 cut(s) 419, 673, 768
SmlI CTYRAG 3 cut(s) 155, 297, 349
SmoI CTYRAG 3 cut(s) 155, 297, 349
SspI AATATT 2 cut(s) 485, 705
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 2 cut(s) 236, 821
StyI CCWWGG 3 cut(s) 36, 744, 855
TaaI ACNGT 2 cut(s) 105, 447
TaiI ACGT 3 cut(s) 430, 684, 756
TaqI TCGA 2 cut(s) 377, 877
Tru1I TTAA 5 cut(s) 383, 471, 512, 693, 898
Tru9I TTAA 5 cut(s) 383, 471, 512, 693, 898
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 1 cut(s) 555
TseI GCWGC 1 cut(s) 32
Tsp45I GTSAC 1 cut(s) 555
TspDTI ATGAA 2 cut(s) 277, 858
TspRI CASTG 1 cut(s) 225
Van91I CCANNNNNTGG 2 cut(s) 544, 828
VpaK11BI GGWCC 3 cut(s) 419, 673, 768
XceI RCATGY 1 cut(s) 556
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.