pycom14g00630

polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009860
Physical Location & Seq
Reverse (-)
412765 .. 418273
5509 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1365 bp
ATGCTTGATGCAATCAAATTACCTCCCCGTGAGCTATATAATCATGTCGAGGAAATTGCCGGTGATGAGTTGATAAGAGCTCTGCAGATTAAGGACAAGATTCCTAGAAGGAAGGCTCTCTTGTCTTTGGAAGGAGAAGTTTTAACTATACTTACAGAAAAGGGATATGTTAGTAAGGATGCAGCTCTTGTAGGCAGTGAAACAATACCAGATATATTTGAAGATGAAGAGGAGGATGAGGAAGTGGTTGTGGATGGTGAAGTAGATGAAGGTGATGTTCATATAAAGCCTAGTTCACGAAAGTCAACCCCCTTGTTCTTTTCTGAGGTAGATGTGAAGCTGGTATTCAAAGAAGTTACATCCAAATTTTTACGAAGACGCATTGTGGAGGGTGGAAGAAGAAGTGATGGACGTACCCCATATGAGATACGTCCAATCAACTCAAAATGTGGACTGCTTCCAAGGGCACATGGAAGTGCTCTTTTCACACGTGGGGAAACCCAGTCATTGGCTGTTGTTACACTCGGGGATAAACAGATGGCACAGAGGGTAGACAACCTAGTAGATGTTGACGAATGGAAGAGATTCTATCTCCAGTACACATTTCCCCCTTCATGTGTTGGGGAAGTTGGACGGAATGGAGCCCCTAGTAGAAGAGAAATTGGCCATGGAATGCTTGCTGAGAGAGCTCTTGAACCAATTTTGCCTTCCGAAGATGATTTTCCTTACACTATACGTGTTGAGAGTACAATTACTGAAAGCAATGGCTCTTCAAGTATGGCATCTGTTTGTGGGGGCTGCTTAGCTTTACAGGATGCTGGTGTTCCAATCAAGTCCTCTATAGTTGGGATAGCAATGGGTATGGTTTTGGAAACTGAGGAATTTGGGGGAAATGGTGCTCCACTAATTCTGTCTGAGATAACTGGATCTGAAGATGCATCCGGGGATATGGATTTCAAGGTTGCCGGAAATGAAGATGGTGTAACTGCATTTCAGATGGACATAAAGGTGAGCGGGATTACATTACCAGTTATGAGAGAGGCACTCCTACAGGCAAAGGATGGCCGTAAGCATATTATTGCGGAAATGTTGAAGTGCTCATCTCCTCCTTCAAGATCGCTTTCTAAGTATGCACCACTGATTCACATAATGAAGGTCAACCCGGAGAAAGTAAACTTAATCATTGGTAGTGGTGGGAAGAAGGTGAAGAGTATTATAGAAGAGTCTGGAGTAGACACAATTGACACACAAGAAGACGGGATAGTGAGAATTACCAGCAGGGATTTGTCTAGCATAGAAAAGGCTAAAGCTATTATTACTAACTTGACAATGGTTCCGTCAGTTGGTGATATCTTCAGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006139 GO:0006396 GO:0006401 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008299 GO:0008408 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010322 GO:0010323 GO:0010467 GO:0010563 GO:0010565 GO:0010675 GO:0010677 GO:0015994 GO:0015995 GO:0016036 GO:0016070 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016119 GO:0016120 GO:0016122 GO:0016123 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0018130 GO:0019216 GO:0019220 GO:0019222 GO:0019438 GO:0019439 GO:0019747 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031425 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033014 GO:0033554 GO:0034641 GO:0034655 GO:0042214 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045827 GO:0045833 GO:0045912 GO:0045936 GO:0046148 GO:0046246 GO:0046483 GO:0046700 GO:0046890 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051174 GO:0051186 GO:0051188 GO:0051716 GO:0062012 GO:0062014 GO:0065007 GO:0071071 GO:0071072 GO:0071496 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1903725 GO:1903726
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko03018 map00230 map00240 map03018
Pfam Domains
Protein Families
KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

455

Amino Acids

49.53

Weight (kDa)

5.03

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 142 - 276 7.1e-30 3' exoribonuclease family, domain 1
KH_1 PF00013 383 - 440 4.6e-08 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 508
AccBSI CCGCTC 1 cut(s) 1014
AccI GTMKAC 2 cut(s) 552, 1233
AciI CCGC 2 cut(s) 1014, 1082
AclWI GGATC 1 cut(s) 934
AcoI YGGCCR 2 cut(s) 664, 1063
AcsI RAATTY 2 cut(s) 365, 881
AcuI CTGAAG 2 cut(s) 951, 1339
AcvI CACGTG 1 cut(s) 491
AfaI GTAC 3 cut(s) 415, 599, 748
AfiI CCNNNNNNNGG 2 cut(s) 508, 1343
AflIII ACRYGT 2 cut(s) 488, 736
AgsI TTSAA 7 cut(s) 221, 349, 695, 774, 958, 1093, 1113
AjuI GAANNNNNNNTTGG 2 cut(s) 691, 723
AluBI AGCT 7 cut(s) 34, 80, 185, 340, 689, 806, 1310
AluI AGCT 7 cut(s) 34, 80, 185, 340, 689, 806, 1310
Alw21I GWGCWC 5 cut(s) 82, 481, 691, 901, 1100
AlwI GGATC 1 cut(s) 934
AlwNI CAGNNNCTG 1 cut(s) 1362
Ama87I CYCGRG 1 cut(s) 524
AoxI GGCC 2 cut(s) 664, 1063
ApeKI GCWGC 2 cut(s) 182, 798
ApoI RAATTY 2 cut(s) 365, 881
Asp700I GAANNNNTTC 1 cut(s) 584
AsuC2I CCSGG 2 cut(s) 943, 1163
AsuHPI GGTGA 6 cut(s) 74, 269, 284, 1021, 1216, 1358
AvaI CYCGRG 1 cut(s) 524
BaeGI GKGCMC 1 cut(s) 469
BalI TGGCCA 1 cut(s) 666
BanII GRGCYC 3 cut(s) 82, 646, 691
BbrPI CACGTG 1 cut(s) 491
BbsI GAAGAC 2 cut(s) 382, 1260
Bbv12I GWGCWC 5 cut(s) 82, 481, 691, 901, 1100
BbvI GCAGC 2 cut(s) 194, 785
BccI CCATC 6 cut(s) 248, 401, 532, 971, 991, 1055
BceAI ACGGC 1 cut(s) 1050
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BcnI CCSGG 2 cut(s) 943, 1163
BfaI CTAG 5 cut(s) 105, 291, 560, 648, 1290
BfmI CTRYAG 3 cut(s) 83, 840, 1049
BisI GCNGC 2 cut(s) 183, 799
BlpI GCTNAGC 1 cut(s) 802
BlsI GCNGC 2 cut(s) 184, 800
Bme1390I CCNGG 2 cut(s) 943, 1163
BmeT110I CYCGRG 1 cut(s) 524
BmiI GGNNCC 2 cut(s) 643, 1335
BmrFI CCNGG 2 cut(s) 943, 1163
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 5 cut(s) 169, 791, 805, 925, 947
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 2 cut(s) 382, 1260
BplI GAGNNNNNCTC 2 cut(s) 1029, 1061
BpmI CTGGAG 2 cut(s) 578, 1248
Bpu1102I GCTNAGC 1 cut(s) 802
BpuMI CCSGG 2 cut(s) 943, 1163
BsaAI YACGTR 2 cut(s) 491, 737
BsaJI CCNNGG 3 cut(s) 461, 667, 942
Bsc4I CCNNNNNNNGG 2 cut(s) 508, 1343
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 4 cut(s) 502, 595, 928, 1028
Bse3DI GCAATG 2 cut(s) 769, 861
BseDI CCNNGG 3 cut(s) 461, 667, 942
BseGI GGATG 7 cut(s) 184, 241, 259, 359, 820, 938, 1066
BseLI CCNNNNNNNGG 2 cut(s) 508, 1343
BseMI GCAATG 2 cut(s) 769, 861
BseMII CTCAG 4 cut(s) 315, 672, 867, 906
BseNI ACTGG 4 cut(s) 502, 595, 928, 1028
BseRI GAGGAG 2 cut(s) 245, 1095
BseSI GKGCMC 1 cut(s) 469
BseXI GCAGC 2 cut(s) 194, 785
BshFI GGCC 2 cut(s) 666, 1065
BsiHKAI GWGCWC 5 cut(s) 82, 481, 691, 901, 1100
BsiHKCI CYCGRG 1 cut(s) 524
BsiSI CCGG 4 cut(s) 60, 942, 966, 1163
BslI CCNNNNNNNGG 2 cut(s) 508, 1343
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 2 cut(s) 666, 1065
BsoBI CYCGRG 1 cut(s) 524
Bsp1286I GDGCHC 7 cut(s) 82, 469, 481, 646, 691, 901, 1100
Bsp143I GATC 2 cut(s) 926, 1115
Bsp1720I GCTNAGC 1 cut(s) 802
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 2 cut(s) 1014, 1082
BspANI GGCC 2 cut(s) 666, 1065
BspCNI CTCAG 4 cut(s) 316, 673, 868, 907
BspLI GGNNCC 2 cut(s) 643, 1335
BspMAI CTGCAG 1 cut(s) 87
BspPI GGATC 1 cut(s) 934
BspQI GCTCTTC 1 cut(s) 775
BsrBI CCGCTC 1 cut(s) 1014
BsrDI GCAATG 2 cut(s) 769, 861
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 4 cut(s) 502, 595, 928, 1028
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 3 cut(s) 461, 667, 942
BssMI GATC 2 cut(s) 926, 1115
BssT1I CCWWGG 2 cut(s) 461, 667
Bst6I CTCTTC 6 cut(s) 222, 575, 649, 775, 1202, 1215
BstBAI YACGTR 2 cut(s) 491, 737
BstC8I GCNNGC 1 cut(s) 678
BstDEI CTNAG 6 cut(s) 324, 681, 802, 876, 915, 1125
BstDSI CCRYGG 1 cut(s) 667
BstF5I GGATG 7 cut(s) 184, 241, 259, 359, 820, 938, 1066
BstKTI GATC 2 cut(s) 929, 1118
BstMBI GATC 2 cut(s) 926, 1115
BstMWI GCNNNNNNNGC 1 cut(s) 686
BstSCI CCNGG 2 cut(s) 941, 1161
BstSFI CTRYAG 3 cut(s) 83, 840, 1049
BstSLI GKGCMC 1 cut(s) 469
BstV1I GCAGC 2 cut(s) 194, 785
BstV2I GAAGAC 2 cut(s) 382, 1260
BstX2I RGATCY 1 cut(s) 926
BstYI RGATCY 1 cut(s) 926
BsuRI GGCC 2 cut(s) 666, 1065
BtgI CCRYGG 1 cut(s) 667
BtsCI GGATG 7 cut(s) 184, 241, 259, 359, 820, 938, 1066
BtsI GCAGTG 1 cut(s) 202
BtsIMutI CAGTG 2 cut(s) 202, 1136
Cac8I GCNNGC 1 cut(s) 678
CaiI CAGNNNCTG 1 cut(s) 1362
Cfr10I RCCGGY 1 cut(s) 59
CseI GACGC 1 cut(s) 387
Csp6I GTAC 3 cut(s) 414, 598, 747
CviAII CATG 4 cut(s) 44, 470, 615, 668
CviQI GTAC 3 cut(s) 414, 598, 747
DdeI CTNAG 6 cut(s) 324, 681, 802, 876, 915, 1125
DpnI GATC 2 cut(s) 928, 1117
DpnII GATC 2 cut(s) 926, 1115
EaeI YGGCCR 2 cut(s) 664, 1063
Eam1104I CTCTTC 6 cut(s) 222, 575, 649, 775, 1202, 1215
EarI CTCTTC 6 cut(s) 222, 575, 649, 775, 1202, 1215
Ecl136II GAGCTC 2 cut(s) 80, 689
Eco130I CCWWGG 2 cut(s) 461, 667
Eco24I GRGCYC 3 cut(s) 82, 646, 691
Eco32I GATATC 1 cut(s) 1351
Eco53kI GAGCTC 2 cut(s) 80, 689
Eco57I CTGAAG 2 cut(s) 951, 1339
Eco72I CACGTG 1 cut(s) 491
Eco88I CYCGRG 1 cut(s) 524
EcoICRI GAGCTC 2 cut(s) 80, 689
EcoRV GATATC 1 cut(s) 1351
EcoT14I CCWWGG 2 cut(s) 461, 667
EcoT22I ATGCAT 1 cut(s) 940
EcoT38I GRGCYC 3 cut(s) 82, 646, 691
ErhI CCWWGG 2 cut(s) 461, 667
FaeI CATG 4 cut(s) 47, 473, 618, 671
FalI AAGNNNNNCTT 2 cut(s) 104, 136
FatI CATG 4 cut(s) 43, 469, 614, 667
FauI CCCGC 1 cut(s) 1007
FauNDI CATATG 1 cut(s) 421
FblI GTMKAC 2 cut(s) 552, 1233
Fnu4HI GCNGC 2 cut(s) 183, 799
FokI GGATG 7 cut(s) 191, 248, 266, 346, 827, 925, 1073
FriOI GRGCYC 3 cut(s) 82, 646, 691
Fsp4HI GCNGC 2 cut(s) 183, 799
FspBI CTAG 5 cut(s) 105, 291, 560, 648, 1290
GluI GCNGC 2 cut(s) 183, 799
GsuI CTGGAG 2 cut(s) 578, 1248
HaeIII GGCC 2 cut(s) 666, 1065
HapII CCGG 4 cut(s) 60, 942, 966, 1163
HgaI GACGC 1 cut(s) 387
Hin1II CATG 4 cut(s) 47, 473, 618, 671
HincII GTYRAC 3 cut(s) 306, 571, 1159
HindII GTYRAC 3 cut(s) 306, 571, 1159
HinfI GANTC 4 cut(s) 100, 585, 1141, 1223
HpaII CCGG 4 cut(s) 60, 942, 966, 1163
HphI GGTGA 6 cut(s) 74, 269, 284, 1021, 1216, 1358
Hpy166II GTNNAC 9 cut(s) 296, 306, 452, 553, 571, 600, 1159, 1174, 1234
Hpy188I TCNGA 5 cut(s) 325, 712, 916, 931, 996
Hpy188III TCNNGA 5 cut(s) 297, 692, 1113, 1227, 1357
Hpy8I GTNNAC 9 cut(s) 296, 306, 452, 553, 571, 600, 1159, 1174, 1234
HpyAV CCTTC 9 cut(s) 102, 106, 125, 263, 621, 717, 1119, 1147, 1195
HpyCH4IV ACGT 4 cut(s) 412, 430, 490, 736
HpyCH4V TGCA 6 cut(s) 11, 85, 182, 938, 989, 1133
HpyF10VI GCNNNNNNNGC 1 cut(s) 686
HpyF3I CTNAG 6 cut(s) 324, 681, 802, 876, 915, 1125
HpySE526I ACGT 4 cut(s) 412, 430, 490, 736
Hsp92II CATG 4 cut(s) 47, 473, 618, 671
Kzo9I GATC 2 cut(s) 926, 1115
LguI GCTCTTC 1 cut(s) 775
LmnI GCTCC 2 cut(s) 641, 904
Lsp1109I GCAGC 2 cut(s) 194, 785
LweI GCATC 5 cut(s) 169, 791, 805, 925, 947
MaeI CTAG 5 cut(s) 105, 291, 560, 648, 1290
MaeII ACGT 4 cut(s) 412, 430, 490, 736
MaeIII GTNAC 3 cut(s) 355, 517, 982
MalI GATC 2 cut(s) 928, 1117
MbiI CCGCTC 1 cut(s) 1014
MboI GATC 2 cut(s) 926, 1115
MfeI CAATTG 1 cut(s) 1239
MflI RGATCY 1 cut(s) 926
MhlI GDGCHC 7 cut(s) 82, 469, 481, 646, 691, 901, 1100
MlsI TGGCCA 1 cut(s) 666
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 1 cut(s) 1232
MmeI TCCRAC 1 cut(s) 610
Mox20I TGGCCA 1 cut(s) 666
Mph1103I ATGCAT 1 cut(s) 940
MroXI GAANNNNTTC 1 cut(s) 584
MscI TGGCCA 1 cut(s) 666
MseI TTAA 3 cut(s) 90, 143, 1178
MslI CAYNNNNRTG 2 cut(s) 474, 1007
Msp20I TGGCCA 1 cut(s) 666
MspI CCGG 4 cut(s) 60, 942, 966, 1163
MspR9I CCNGG 2 cut(s) 943, 1163
MunI CAATTG 1 cut(s) 1239
Mva1269I GAATGC 1 cut(s) 678
MwoI GCNNNNNNNGC 1 cut(s) 686
NciI CCSGG 2 cut(s) 943, 1163
NcoI CCATGG 1 cut(s) 667
NdeI CATATG 1 cut(s) 421
NdeII GATC 2 cut(s) 926, 1115
NlaIII CATG 4 cut(s) 47, 473, 618, 671
NlaIV GGNNCC 2 cut(s) 643, 1335
NsiI ATGCAT 1 cut(s) 940
PciSI GCTCTTC 1 cut(s) 775
PctI GAATGC 1 cut(s) 678
PdmI GAANNNNTTC 1 cut(s) 584
PfeI GAWTC 3 cut(s) 100, 585, 1141
PflMI CCANNNNNTGG 1 cut(s) 508
PkrI GCNGC 2 cut(s) 184, 800
PleI GAGTC 1 cut(s) 1231
PmaCI CACGTG 1 cut(s) 491
PmlI CACGTG 1 cut(s) 491
PpsI GAGTC 1 cut(s) 1231
Ppu21I YACGTR 2 cut(s) 491, 737
Psp124BI GAGCTC 2 cut(s) 82, 691
PspCI CACGTG 1 cut(s) 491
PspN4I GGNNCC 2 cut(s) 643, 1335
PstI CTGCAG 1 cut(s) 87
PstNI CAGNNNCTG 1 cut(s) 1362
PsuI RGATCY 1 cut(s) 926
RsaI GTAC 3 cut(s) 415, 599, 748
RsaNI GTAC 3 cut(s) 414, 598, 747
RseI CAYNNNNRTG 2 cut(s) 474, 1007
SacI GAGCTC 2 cut(s) 82, 691
SapI GCTCTTC 1 cut(s) 775
SaqAI TTAA 3 cut(s) 90, 143, 1178
SatI GCNGC 2 cut(s) 183, 799
Sau3AI GATC 2 cut(s) 926, 1115
SchI GAGTC 1 cut(s) 1232
ScrFI CCNGG 2 cut(s) 943, 1163
SduI GDGCHC 7 cut(s) 82, 469, 481, 646, 691, 901, 1100
SfaNI GCATC 5 cut(s) 169, 791, 805, 925, 947
SfcI CTRYAG 3 cut(s) 83, 840, 1049
SmiMI CAYNNNNRTG 2 cut(s) 474, 1007
SsiI CCGC 2 cut(s) 1014, 1082
SspMI CTAG 5 cut(s) 105, 291, 560, 648, 1290
SstI GAGCTC 2 cut(s) 82, 691
StyD4I CCNGG 2 cut(s) 941, 1161
StyI CCWWGG 2 cut(s) 461, 667
TaiI ACGT 4 cut(s) 415, 433, 493, 739
TaqI TCGA 1 cut(s) 48
TatI WGTACW 2 cut(s) 597, 746
TfiI GAWTC 3 cut(s) 100, 585, 1141
Tru1I TTAA 3 cut(s) 90, 143, 1178
Tru9I TTAA 3 cut(s) 90, 143, 1178
TscAI CASTG 2 cut(s) 202, 1143
TseI GCWGC 2 cut(s) 182, 798
TspDTI ATGAA 6 cut(s) 240, 269, 282, 603, 987, 1166
TspGWI ACGGA 2 cut(s) 649, 1326
TspRI CASTG 2 cut(s) 202, 1143
Van91I CCANNNNNTGG 1 cut(s) 508
XapI RAATTY 2 cut(s) 365, 881
XmiI GTMKAC 2 cut(s) 552, 1233
XmnI GAANNNNTTC 1 cut(s) 584
XspI CTAG 5 cut(s) 105, 291, 560, 648, 1290
Zsp2I ATGCAT 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.