pycom14g03320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013698
Physical Location & Seq
Reverse (-)
2652497 .. 2653255
759 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGTCATCTCGCCGGAGACGGGAGAGAGTAGAGATGATGGAGGCCGAAGGAGGTGTCTCGTTCCCTGTCGTCTTCTCCGATGGCGAGACCGAGACCGATATCGGCGACCTCGTCGTTTACCCAAAGCTTGATTTCCCACGGTTCCTGTCGGTTCTGAGCCACAAGATCGGAATCTTGCCGGGCCAGTTCACTGTTTTCTTATCCTCGCCGGAGACCCGTCAGCGAATCCCCATCACCGGCAAGGTCAACTTCAGCGCGATTTCTCGCGAGAAGAACTGCTGCTTCCTCGTGGAGCTCAAGCGGTCGAGGCGGCGGAAGAATCACAATTACCATGTCCACCACCACCAAGACTTCCAAGAAGACGAGCACCACAACACGGTGTCGTTTGGAGCTCAAAATCCGATCAACAAGAATCTCCATCCCGAGAATGTTATGCTTTTAAAACGCGGCATGGAGATCGAAAAAGGAAACGCCGCCGGATTGACCATGCCTTTTGCGGGTCGGGTCGAGTACGAGAATGGGGCCAGGGAGCAGCAGTCTGGGAAAGAGAGGTACTTGACGGACATGGGGCTGGGACGGGCCGATGGGCTCGGGATTGGGCTGGGCCGCCGTAGTGGAGCGACGGCGTGCGAGGAGTGTTCGAGGGCGAACGAGATGGGGAGAGAAGTCGGGTTCCACTGGTGCGCTTACGACCCTGTGACTTTCGGGTTCAGGTCGCCGGCGGGTCCGATTTCCCGACCCGTTAAACGATCCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

253

Amino Acids

28.41

Weight (kDa)

8.83

Isoelectric Point (pI)

65.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7138 PF23596 18 - 97 7.7e-37 Domain of unknown function (DUF7138)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20260
malus_domestica MD12G1039700.v1.1 MD14G1038300.v1.1
prunus_persica Prupe.7G095200_v2.0.a1
pyrus_communis pycom03g00540 pycom12g03370 pycom14g03320
rosa_chinensis RchiOBHm_Chr3g0475851
rosa_laevigata RLG00000023807
rosa_multiflora Rmu_co8277165.1_g000001
rosa_roxburghii Rroxscaffold_6G00405790
rosa_rugosa Rorug03G0151700
rosa_samantha Rh3BG232000 Rh3CG226600 Rh3DG227200 Rh4CG218200 Rh7BG133300
rosa_wichuraiana Rw3G018360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 257, 267, 447
AciI CCGC 8 cut(s) 301, 310, 313, 447, 474, 497, 607, 722
AclWI GGATC 1 cut(s) 744
AcuI CTGAAG 1 cut(s) 235
AfaI GTAC 2 cut(s) 512, 554
AfiI CCNNNNNNNGG 4 cut(s) 19, 236, 376, 497
AjnI CCWGG 1 cut(s) 524
AluBI AGCT 3 cut(s) 127, 295, 392
AluI AGCT 3 cut(s) 127, 295, 392
Alw21I GWGCWC 3 cut(s) 297, 369, 394
Alw26I GTCTC 5 cut(s) 10, 61, 80, 86, 206
AlwI GGATC 1 cut(s) 744
Ama87I CYCGRG 2 cut(s) 422, 590
AoxI GGCC 5 cut(s) 42, 181, 522, 579, 604
ApeKI GCWGC 2 cut(s) 279, 532
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
AspLEI GCGC 2 cut(s) 257, 686
AspS9I GGNCC 5 cut(s) 181, 522, 579, 604, 725
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 1 cut(s) 226
AvaI CYCGRG 2 cut(s) 422, 590
AvaII GGWCC 1 cut(s) 725
BanII GRGCYC 3 cut(s) 297, 394, 591
BauI CACGAG 1 cut(s) 287
BbsI GAAGAC 2 cut(s) 64, 366
Bbv12I GWGCWC 3 cut(s) 297, 369, 394
BbvI GCAGC 2 cut(s) 266, 544
BccI CCATC 6 cut(s) 31, 74, 239, 426, 578, 649
BceAI ACGGC 2 cut(s) 594, 639
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BciT130I CCWGG 1 cut(s) 526
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 5 cut(s) 10, 61, 80, 86, 206
BfaI CTAG 1 cut(s) 757
BisI GCNGC 6 cut(s) 280, 311, 448, 474, 533, 607
BlsI GCNGC 6 cut(s) 281, 312, 449, 475, 534, 608
Bme1390I CCNGG 2 cut(s) 180, 526
Bme18I GGWCC 1 cut(s) 725
BmeT110I CYCGRG 2 cut(s) 422, 590
BmgT120I GGNCC 5 cut(s) 181, 522, 579, 604, 725
BmiI GGNNCC 4 cut(s) 143, 523, 674, 726
BmrFI CCNGG 2 cut(s) 180, 526
BpiI GAAGAC 2 cut(s) 64, 366
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 180
BsaBI GATNNNNATC 1 cut(s) 170
BsaI GGTCTC 3 cut(s) 80, 86, 206
BsaJI CCNNGG 2 cut(s) 137, 525
BsaXI ACNNNNNCTCC 2 cut(s) 521, 551
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 236, 376, 497
Bse118I RCCGGY 2 cut(s) 236, 718
Bse1I ACTGG 2 cut(s) 184, 683
Bse8I GATNNNNATC 1 cut(s) 170
BseBI CCWGG 1 cut(s) 526
BseDI CCNNGG 2 cut(s) 137, 525
BseGI GGATG 1 cut(s) 418
BseJI GATNNNNATC 1 cut(s) 170
BseLI CCNNNNNNNGG 4 cut(s) 19, 236, 376, 497
BseMII CTCAG 1 cut(s) 146
BseNI ACTGG 2 cut(s) 184, 683
BseRI GAGGAG 1 cut(s) 647
BseXI GCAGC 2 cut(s) 266, 544
BseYI CCCAGC 2 cut(s) 571, 601
Bsh1236I CGCG 3 cut(s) 257, 267, 447
Bsh1285I CGRYCG 1 cut(s) 305
BshFI GGCC 5 cut(s) 44, 183, 524, 581, 606
BsiEI CGRYCG 1 cut(s) 305
BsiHKAI GWGCWC 3 cut(s) 297, 369, 394
BsiHKCI CYCGRG 2 cut(s) 422, 590
BsiSI CCGG 6 cut(s) 13, 179, 209, 237, 477, 719
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 4 cut(s) 19, 236, 376, 497
BsmAI GTCTC 5 cut(s) 10, 61, 80, 86, 206
BsmBI CGTCTC 1 cut(s) 10
BsmFI GGGAC 1 cut(s) 588
BsnI GGCC 5 cut(s) 44, 183, 524, 581, 606
Bso31I GGTCTC 3 cut(s) 80, 86, 206
BsoBI CYCGRG 2 cut(s) 422, 590
Bsp1286I GDGCHC 4 cut(s) 297, 369, 394, 591
Bsp143I GATC 4 cut(s) 165, 402, 456, 749
Bsp68I TCGCGA 1 cut(s) 267
BspACI CCGC 8 cut(s) 301, 310, 313, 447, 474, 497, 607, 722
BspANI GGCC 5 cut(s) 44, 183, 524, 581, 606
BspCNI CTCAG 1 cut(s) 147
BspFNI CGCG 3 cut(s) 257, 267, 447
BspLI GGNNCC 4 cut(s) 143, 523, 674, 726
BspPI GGATC 1 cut(s) 744
BspTNI GGTCTC 3 cut(s) 80, 86, 206
BsrFI RCCGGY 2 cut(s) 236, 718
BsrI ACTGG 2 cut(s) 184, 683
BssAI RCCGGY 2 cut(s) 236, 718
BssECI CCNNGG 2 cut(s) 137, 525
BssMI GATC 4 cut(s) 165, 402, 456, 749
BssSI CACGAG 1 cut(s) 287
Bst2BI CACGAG 1 cut(s) 287
Bst2UI CCWGG 1 cut(s) 526
Bst4CI ACNGT 3 cut(s) 141, 193, 379
BstC8I GCNNGC 2 cut(s) 628, 720
BstDEI CTNAG 1 cut(s) 155
BstDSI CCRYGG 1 cut(s) 137
BstF5I GGATG 1 cut(s) 418
BstFNI CGCG 3 cut(s) 257, 267, 447
BstHHI GCGC 2 cut(s) 257, 686
BstKTI GATC 4 cut(s) 168, 405, 459, 752
BstMAI GTCTC 5 cut(s) 10, 61, 80, 86, 206
BstMBI GATC 4 cut(s) 165, 402, 456, 749
BstMCI CGRYCG 1 cut(s) 305
BstMWI GCNNNNNNNGC 1 cut(s) 307
BstNI CCWGG 1 cut(s) 526
BstSCI CCNGG 2 cut(s) 178, 524
BstUI CGCG 3 cut(s) 257, 267, 447
BstV1I GCAGC 2 cut(s) 266, 544
BstV2I GAAGAC 2 cut(s) 64, 366
BsuRI GGCC 5 cut(s) 44, 183, 524, 581, 606
BtgI CCRYGG 1 cut(s) 137
BtsCI GGATG 1 cut(s) 418
BtsIMutI CAGTG 2 cut(s) 189, 676
BtuMI TCGCGA 1 cut(s) 267
Cac8I GCNNGC 2 cut(s) 628, 720
CfoI GCGC 2 cut(s) 257, 686
Cfr10I RCCGGY 2 cut(s) 236, 718
Cfr13I GGNCC 5 cut(s) 181, 522, 579, 604, 725
Csp6I GTAC 2 cut(s) 511, 553
CviAII CATG 4 cut(s) 332, 451, 487, 565
CviQI GTAC 2 cut(s) 511, 553
DdeI CTNAG 1 cut(s) 155
DpnI GATC 4 cut(s) 167, 404, 458, 751
DpnII GATC 4 cut(s) 165, 402, 456, 749
DraI TTTAAA 1 cut(s) 441
EciI GGCGGA 1 cut(s) 328
Ecl136II GAGCTC 2 cut(s) 295, 392
Eco24I GRGCYC 3 cut(s) 297, 394, 591
Eco31I GGTCTC 3 cut(s) 80, 86, 206
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 725
Eco53kI GAGCTC 2 cut(s) 295, 392
Eco57I CTGAAG 1 cut(s) 235
Eco88I CYCGRG 2 cut(s) 422, 590
EcoICRI GAGCTC 2 cut(s) 295, 392
EcoRII CCWGG 1 cut(s) 524
EcoRV GATATC 1 cut(s) 100
EcoT38I GRGCYC 3 cut(s) 297, 394, 591
Esp3I CGTCTC 1 cut(s) 10
FaeI CATG 4 cut(s) 335, 454, 490, 568
FaiI YATR 5 cut(s) 333, 434, 452, 488, 566
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 588
FatI CATG 4 cut(s) 331, 450, 486, 564
FauI CCCGC 2 cut(s) 490, 715
Fnu4HI GCNGC 6 cut(s) 280, 311, 448, 474, 533, 607
FokI GGATG 1 cut(s) 405
FriOI GRGCYC 3 cut(s) 297, 394, 591
Fsp4HI GCNGC 6 cut(s) 280, 311, 448, 474, 533, 607
FspBI CTAG 1 cut(s) 757
GlaI GCGC 2 cut(s) 256, 685
GluI GCNGC 6 cut(s) 280, 311, 448, 474, 533, 607
GsaI CCCAGC 2 cut(s) 575, 605
HaeIII GGCC 5 cut(s) 44, 183, 524, 581, 606
HapII CCGG 6 cut(s) 13, 179, 209, 237, 477, 719
HhaI GCGC 2 cut(s) 257, 686
Hin1II CATG 4 cut(s) 335, 454, 490, 568
Hin6I GCGC 2 cut(s) 255, 684
HinP1I GCGC 2 cut(s) 255, 684
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 4 cut(s) 171, 225, 319, 412
HpaII CCGG 6 cut(s) 13, 179, 209, 237, 477, 719
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 4 cut(s) 118, 189, 247, 337
Hpy188I TCNGA 6 cut(s) 79, 156, 170, 402, 729, 754
Hpy188III TCNNGA 4 cut(s) 266, 422, 592, 735
Hpy8I GTNNAC 4 cut(s) 118, 189, 247, 337
Hpy99I CGWCG 2 cut(s) 116, 625
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 3 cut(s) 141, 193, 379
HpyF10VI GCNNNNNNNGC 1 cut(s) 307
HpyF3I CTNAG 1 cut(s) 155
Hsp92II CATG 4 cut(s) 335, 454, 490, 568
HspAI GCGC 2 cut(s) 255, 684
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
Kzo9I GATC 4 cut(s) 165, 402, 456, 749
LmnI GCTCC 4 cut(s) 292, 389, 529, 617
Lsp1109I GCAGC 2 cut(s) 266, 544
MaeI CTAG 1 cut(s) 757
MaeIII GTNAC 1 cut(s) 697
MalI GATC 4 cut(s) 167, 404, 458, 751
MboI GATC 4 cut(s) 165, 402, 456, 749
MboII GAAGA 4 cut(s) 64, 283, 328, 371
MhlI GDGCHC 4 cut(s) 297, 369, 394, 591
MluCI AATT 1 cut(s) 325
MnlI CCTC 9 cut(s) 34, 44, 119, 214, 296, 300, 543, 625, 636
MreI CGCCGGCG 1 cut(s) 718
MroNI GCCGGC 1 cut(s) 718
MseI TTAA 2 cut(s) 440, 744
MspI CCGG 6 cut(s) 13, 179, 209, 237, 477, 719
MspR9I CCNGG 2 cut(s) 180, 526
MvaI CCWGG 1 cut(s) 526
MvnI CGCG 3 cut(s) 257, 267, 447
MwoI GCNNNNNNNGC 1 cut(s) 307
NaeI GCCGGC 1 cut(s) 720
NciI CCSGG 1 cut(s) 180
NdeII GATC 4 cut(s) 165, 402, 456, 749
NgoMIV GCCGGC 1 cut(s) 718
NlaIII CATG 4 cut(s) 335, 454, 490, 568
NlaIV GGNNCC 4 cut(s) 143, 523, 674, 726
NmuCI GTSAC 1 cut(s) 697
NruI TCGCGA 1 cut(s) 267
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 108
PdiI GCCGGC 1 cut(s) 720
PfeI GAWTC 4 cut(s) 171, 225, 319, 412
PflFI GACNNNGTC 1 cut(s) 110
PkrI GCNGC 6 cut(s) 281, 312, 449, 475, 534, 608
Psp124BI GAGCTC 2 cut(s) 297, 394
Psp6I CCWGG 1 cut(s) 524
PspFI CCCAGC 2 cut(s) 571, 601
PspGI CCWGG 1 cut(s) 524
PspN4I GGNNCC 4 cut(s) 143, 523, 674, 726
PspPI GGNCC 5 cut(s) 181, 522, 579, 604, 725
PsyI GACNNNGTC 1 cut(s) 110
RruI TCGCGA 1 cut(s) 267
RsaI GTAC 2 cut(s) 512, 554
RsaNI GTAC 2 cut(s) 511, 553
SacI GAGCTC 2 cut(s) 297, 394
SaqAI TTAA 2 cut(s) 440, 744
SatI GCNGC 6 cut(s) 280, 311, 448, 474, 533, 607
Sau3AI GATC 4 cut(s) 165, 402, 456, 749
Sau96I GGNCC 5 cut(s) 181, 522, 579, 604, 725
ScrFI CCNGG 2 cut(s) 180, 526
SduI GDGCHC 4 cut(s) 297, 369, 394, 591
SetI ASST 8 cut(s) 55, 111, 129, 246, 297, 394, 554, 716
SgrAI CRCCGGYG 1 cut(s) 718
SinI GGWCC 1 cut(s) 725
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 1 cut(s) 325
SsiI CCGC 8 cut(s) 301, 310, 313, 447, 474, 497, 607, 722
SspMI CTAG 1 cut(s) 757
SstI GAGCTC 2 cut(s) 297, 394
StyD4I CCNGG 2 cut(s) 178, 524
TaaI ACNGT 3 cut(s) 141, 193, 379
TaqI TCGA 4 cut(s) 305, 459, 507, 641
TaqII GACCGA 2 cut(s) 104, 110
TasI AATT 1 cut(s) 325
TauI GCSGC 4 cut(s) 313, 450, 476, 609
TfiI GAWTC 4 cut(s) 171, 225, 319, 412
Tru1I TTAA 2 cut(s) 440, 744
Tru9I TTAA 2 cut(s) 440, 744
TscAI CASTG 2 cut(s) 196, 683
TseFI GTSAC 1 cut(s) 697
TseI GCWGC 2 cut(s) 279, 532
Tsp45I GTSAC 1 cut(s) 697
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 2 cut(s) 196, 683
Tth111I GACNNNGTC 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 725
XspI CTAG 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.