pycom14g04300

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011155
Physical Location & Seq
Forward (+)
3712864 .. 3717442
4579 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
ATGTGGTTGATCGAACGTAAGTGGCTATTGAACCTTTCAGACAAAAGCTTGCAATTAATTGCCCAGAATTATCCAGAATTACAGTTGTTGAACCTGACAAGGTGTGTCAAGTTAACAGATTCTGGCTTGCAGCAGATATTGCACGGTTGTCCTAGTCTCCATAGTCTAAATTTGTACGCCCTGTCAAGCTTCACTGATGAAGCTTACAAGAAAATATCACTTCTTTCCCATCTTAAATTCTTGGATTTATGTGGCGGCCAGAATCTGTCAGATGAAGGGGTGGCTTGTATAGCTAGATGCAAGGGCCTTCTTTCTCTCAATTTGACATGGTGTGTACGGGTCACTGACGTGGGGGTCATAGCCGTTGCGCAGGGTTGCACATCTCTTGAATTTCTCAGCTTATTTGGGATAGTTGGGGTGACCGATAAATGCTTGGAGGCCCTTTCACTGACCTGCTCAAACACACTTACAACTCTAGATGTTAATGGATGTATTGGAATTAAGAAACGGAGCCGTGATGAATTGCTTCAGATGTTTCCAAAGTTACGGTGCTTCAAAGTGCACAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

190

Amino Acids

20.95

Weight (kDa)

8.29

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 51 - 167 1.4e-09 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58530
fragaria_vesca FvH4_6g21620 FvH4_6g21620
malus_domestica MD12G1053200.v1.1 MD14G1051600.v1.1
prunus_persica Prupe.7G084400_v2.0.a1
pyrus_communis pycom12g04660 pycom14g04290 pycom14g04300
rosa_chinensis RchiOBHm_Chr3g0478871
rosa_laevigata RLG00000023619
rosa_multiflora Rmu_sc0004831.1_g000004 Rmu_sc0005751.1_g000013
rosa_roxburghii Rroxscaffold_6G00403010
rosa_rugosa Rorug03G0168800
rosa_samantha Rh3AG220400 Rh3BG252300 Rh3CG249100 Rh3DG245300
rosa_wichuraiana Rw3G019820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
Acc16I TGCGCA 1 cut(s) 369
Acc36I ACCTGC 1 cut(s) 461
AciI CCGC 1 cut(s) 255
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 3 cut(s) 169, 236, 389
AcuI CTGAAG 1 cut(s) 512
AfaI GTAC 2 cut(s) 176, 336
AgsI TTSAA 4 cut(s) 31, 91, 389, 556
AjiI CACGTC 1 cut(s) 349
AleI CACNNNNGTG 1 cut(s) 347
AluBI AGCT 6 cut(s) 48, 189, 203, 293, 399, 567
AluI AGCT 6 cut(s) 48, 189, 203, 293, 399, 567
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 1 cut(s) 161
Alw44I GTGCAC 1 cut(s) 560
AlwNI CAGNNNCTG 2 cut(s) 122, 265
AoxI GGCC 3 cut(s) 256, 304, 438
ApaLI GTGCAC 1 cut(s) 560
ApeKI GCWGC 1 cut(s) 130
ApoI RAATTY 3 cut(s) 169, 236, 389
AseI ATTAAT 1 cut(s) 56
Asp700I GAANNNNTTC 1 cut(s) 525
AspLEI GCGC 1 cut(s) 370
AspS9I GGNCC 2 cut(s) 304, 439
AsuHPI GGTGA 1 cut(s) 430
BaeGI GKGCMC 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 1 cut(s) 142
BccI CCATC 1 cut(s) 237
BceAI ACGGC 2 cut(s) 347, 498
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 3 cut(s) 153, 294, 476
BfuAI ACCTGC 1 cut(s) 461
BisI GCNGC 2 cut(s) 131, 256
BlsI GCNGC 2 cut(s) 132, 257
BmgBI CACGTC 1 cut(s) 349
BmgT120I GGNCC 2 cut(s) 304, 439
BmiI GGNNCC 1 cut(s) 512
BmsI GCATC 1 cut(s) 287
BseGI GGATG 1 cut(s) 494
BseMII CTCAG 1 cut(s) 409
BseSI GKGCMC 1 cut(s) 564
BseXI GCAGC 1 cut(s) 142
BshFI GGCC 3 cut(s) 258, 306, 440
BsiHKAI GWGCWC 1 cut(s) 564
BsmAI GTCTC 1 cut(s) 161
BsnI GGCC 3 cut(s) 258, 306, 440
Bsp1286I GDGCHC 1 cut(s) 564
Bsp143I GATC 1 cut(s) 9
BspACI CCGC 1 cut(s) 255
BspANI GGCC 3 cut(s) 258, 306, 440
BspCNI CTCAG 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 512
BspMI ACCTGC 1 cut(s) 461
BssMI GATC 1 cut(s) 9
Bst4CI ACNGT 3 cut(s) 84, 146, 549
BstAPI GCANNNNNTGC 1 cut(s) 139
BstC8I GCNNGC 2 cut(s) 50, 128
BstDEI CTNAG 1 cut(s) 395
BstEII GGTNACC 1 cut(s) 418
BstF5I GGATG 1 cut(s) 494
BstHHI GCGC 1 cut(s) 370
BstKTI GATC 1 cut(s) 12
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 1 cut(s) 9
BstMWI GCNNNNNNNGC 2 cut(s) 139, 290
BstPI GGTNACC 1 cut(s) 418
BstSLI GKGCMC 1 cut(s) 564
BstV1I GCAGC 1 cut(s) 142
BsuRI GGCC 3 cut(s) 258, 306, 440
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 1 cut(s) 494
BtsIMutI CAGTG 3 cut(s) 192, 342, 446
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 2 cut(s) 50, 128
CaiI CAGNNNCTG 2 cut(s) 122, 265
CfoI GCGC 1 cut(s) 370
Cfr13I GGNCC 2 cut(s) 304, 439
Csp6I GTAC 2 cut(s) 175, 335
CviAII CATG 1 cut(s) 327
CviQI GTAC 2 cut(s) 175, 335
DdeI CTNAG 1 cut(s) 395
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 512
Eco91I GGTNACC 1 cut(s) 418
EcoO109I RGGNCCY 2 cut(s) 304, 439
EcoO65I GGTNACC 1 cut(s) 418
FaeI CATG 1 cut(s) 330
FaiI YATR 5 cut(s) 162, 250, 290, 328, 359
FatI CATG 1 cut(s) 326
Fnu4HI GCNGC 2 cut(s) 131, 256
FokI GGATG 1 cut(s) 501
Fsp4HI GCNGC 2 cut(s) 131, 256
FspBI CTAG 3 cut(s) 153, 294, 476
FspI TGCGCA 1 cut(s) 369
GlaI GCGC 1 cut(s) 369
GluI GCNGC 2 cut(s) 131, 256
HaeIII GGCC 3 cut(s) 258, 306, 440
HhaI GCGC 1 cut(s) 370
Hin1II CATG 1 cut(s) 330
Hin6I GCGC 1 cut(s) 368
HinP1I GCGC 1 cut(s) 368
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HindIII AAGCTT 3 cut(s) 46, 187, 201
HinfI GANTC 2 cut(s) 119, 262
HpaI GTTAAC 1 cut(s) 114
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 3 cut(s) 114, 335, 562
Hpy188I TCNGA 3 cut(s) 40, 271, 531
Hpy188III TCNNGA 3 cut(s) 74, 386, 476
Hpy8I GTNNAC 3 cut(s) 114, 335, 562
HpyAV CCTTC 2 cut(s) 269, 317
HpyCH4III ACNGT 3 cut(s) 84, 146, 549
HpyCH4IV ACGT 2 cut(s) 16, 348
HpyCH4V TGCA 6 cut(s) 52, 130, 142, 300, 378, 562
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 290
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 2 cut(s) 16, 348
Hsp92II CATG 1 cut(s) 330
HspAI GCGC 1 cut(s) 368
KspAI GTTAAC 1 cut(s) 114
Kzo9I GATC 1 cut(s) 9
LmnI GCTCC 1 cut(s) 510
LpnPI CCDG 8 cut(s) 77, 87, 107, 108, 194, 272, 356, 466
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 1 cut(s) 287
MaeI CTAG 3 cut(s) 153, 294, 476
MaeII ACGT 2 cut(s) 16, 348
MaeIII GTNAC 3 cut(s) 340, 418, 543
MalI GATC 1 cut(s) 11
MboI GATC 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 564
MnlI CCTC 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 5 cut(s) 56, 113, 234, 483, 501
MslI CAYNNNNRTG 1 cut(s) 347
MwoI GCNNNNNNNGC 2 cut(s) 139, 290
NdeII GATC 1 cut(s) 9
NlaIII CATG 1 cut(s) 330
NlaIV GGNNCC 1 cut(s) 512
NmuCI GTSAC 2 cut(s) 340, 418
NsbI TGCGCA 1 cut(s) 369
OliI CACNNNNGTG 1 cut(s) 347
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 2 cut(s) 119, 262
PkrI GCNGC 2 cut(s) 132, 257
PshBI ATTAAT 1 cut(s) 56
PspEI GGTNACC 1 cut(s) 418
PspN4I GGNNCC 1 cut(s) 512
PspPI GGNCC 2 cut(s) 304, 439
PstNI CAGNNNCTG 2 cut(s) 122, 265
RsaI GTAC 2 cut(s) 176, 336
RsaNI GTAC 2 cut(s) 175, 335
RseI CAYNNNNRTG 1 cut(s) 347
SaqAI TTAA 5 cut(s) 56, 113, 234, 483, 501
SatI GCNGC 2 cut(s) 131, 256
Sau3AI GATC 1 cut(s) 9
Sau96I GGNCC 2 cut(s) 304, 439
SduI GDGCHC 1 cut(s) 564
SfaNI GCATC 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 347
SsiI CCGC 1 cut(s) 255
SspMI CTAG 3 cut(s) 153, 294, 476
TaaI ACNGT 3 cut(s) 84, 146, 549
TaiI ACGT 2 cut(s) 19, 351
TaqI TCGA 1 cut(s) 12
TaqII GACCGA 1 cut(s) 437
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 2 cut(s) 119, 262
Tru1I TTAA 5 cut(s) 56, 113, 234, 483, 501
Tru9I TTAA 5 cut(s) 56, 113, 234, 483, 501
TscAI CASTG 3 cut(s) 199, 349, 453
TseFI GTSAC 2 cut(s) 340, 418
TseI GCWGC 1 cut(s) 130
Tsp45I GTSAC 2 cut(s) 340, 418
TspDTI ATGAA 3 cut(s) 213, 288, 534
TspGWI ACGGA 1 cut(s) 523
TspRI CASTG 3 cut(s) 199, 349, 453
VneI GTGCAC 1 cut(s) 560
VspI ATTAAT 1 cut(s) 56
XapI RAATTY 3 cut(s) 169, 236, 389
XbaI TCTAGA 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 3 cut(s) 153, 294, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.