pycom14g05310

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010291
Physical Location & Seq
Forward (+)
5082799 .. 5085310
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05310.5

Sequence Viewer

Length: 885 bp
ATGGGGAAGAGAAAGAGAGCTAGGGTTTCTGAGCAGAAGCAGCAACCACCACCACAAGTCGAAGACGAAGAAGAGGAGCACGAACGGAGCGACGAAGATCGCGACCCCCCGACTTCCACCAACGAGAAGAGCCTCTATGAGGTTCTTGGCGTGGAGAGAAGCGCTACTCAGCAGGAAATAAAGAAAGCCTATCATAAATTAGCATTGCGATTGCATCCGGATAAGAATCCCGGTGATGAGGAAGCTAAAGAAAAGTTTCAGCAATTGCAAAAGGTGATATCGATTCTCGGGGATGAGGAGAAACGAGCACTCTATGACGAGACGGGTTGCCTTGATGATGATGACCTTACTGGTGATGTTGTTCAGAATCTGCATGAATATTTCCGGGTCATATATAAAAAGGTCACTGAAGCTGATATCGAAGAGTTTGAAGCAAACTACAGGGGATCTGATTCCGAGAAGAAAGATTTGATTGATCTGTATAGCCAGTGCAAGGGGAATATGAACAGACTATTCTGTTCGATGATATGTTCTGATCCTAAACTCGATTCACACCGCTTCAAGGATATTCTTGATGAGGCAATAGCTGCAGGAGAACTGAAATCAACCAAAGCCTACATGAAATGGGCAAAGAAAGTATCTGAAACAAAACCTCCTACCAGCCCTTTAAGGAGAAAGACCAAGGCAAAGAAACAGACAGAAGATCTGTATGCAATCATATCTCAGCGCCGCAGTGAGAGGAAAAACCAGTTTGATTCGATGTTTTCTTCTCTAGTTTCCAGATACGGGGGGAACACTTCTTCAGAACCCACGGAAGAAGAATTTGAGGCTACACAGAAAAAACTTGAAAGAAAAAGGTCCTCCACGAAATCAAAGCGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

295

Amino Acids

34.08

Weight (kDa)

6.59

Isoelectric Point (pI)

58.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 102
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 2 cut(s) 556, 730
AclWI GGATC 2 cut(s) 454, 530
AcsI RAATTY 1 cut(s) 821
AcuI CTGAAG 2 cut(s) 429, 786
AfeI AGCGCT 1 cut(s) 163
AfiI CCNNNNNNNGG 4 cut(s) 139, 493, 562, 786
AgsI TTSAA 3 cut(s) 431, 562, 848
AluBI AGCT 4 cut(s) 20, 245, 413, 587
AluI AGCT 4 cut(s) 20, 245, 413, 587
Alw21I GWGCWC 2 cut(s) 81, 310
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 2 cut(s) 454, 530
AlwNI CAGNNNCTG 1 cut(s) 370
Ama87I CYCGRG 1 cut(s) 287
Aor13HI TCCGGA 1 cut(s) 217
Aor51HI AGCGCT 1 cut(s) 163
ApeKI GCWGC 2 cut(s) 40, 587
ApoI RAATTY 1 cut(s) 821
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 3 cut(s) 164, 729, 879
AspS9I GGNCC 1 cut(s) 858
AsuC2I CCSGG 2 cut(s) 231, 386
AsuHPI GGTGA 3 cut(s) 245, 286, 365
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 858
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 81, 310
BbvI GCAGC 2 cut(s) 52, 574
BcnI CCSGG 2 cut(s) 231, 386
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 2 cut(s) 21, 773
BfmI CTRYAG 2 cut(s) 439, 588
BfoI RGCGCY 2 cut(s) 165, 730
BglII AGATCT 1 cut(s) 703
BisI GCNGC 3 cut(s) 41, 588, 730
BlsI GCNGC 3 cut(s) 42, 589, 731
Bme1390I CCNGG 2 cut(s) 231, 386
Bme18I GGWCC 1 cut(s) 858
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 858
BmrFI CCNGG 2 cut(s) 231, 386
BmsI GCATC 1 cut(s) 223
BpiI GAAGAC 1 cut(s) 69
BpuMI CCSGG 2 cut(s) 231, 386
Bsa29I ATCGAT 1 cut(s) 281
BsaBI GATNNNNATC 1 cut(s) 225
BsaJI CCNNGG 2 cut(s) 681, 810
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 4 cut(s) 139, 493, 562, 786
Bse1I ACTGG 3 cut(s) 355, 487, 748
Bse3DI GCAATG 1 cut(s) 203
Bse8I GATNNNNATC 1 cut(s) 225
BseAI TCCGGA 1 cut(s) 217
BseCI ATCGAT 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 681, 810
BseGI GGATG 2 cut(s) 214, 298
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 4 cut(s) 139, 493, 562, 786
BseMI GCAATG 1 cut(s) 203
BseMII CTCAG 3 cut(s) 21, 182, 737
BseNI ACTGG 3 cut(s) 355, 487, 748
BseRI GAGGAG 2 cut(s) 89, 311
BseXI GCAGC 2 cut(s) 52, 574
Bsh1236I CGCG 1 cut(s) 102
BshVI ATCGAT 1 cut(s) 281
BsiHKAI GWGCWC 2 cut(s) 81, 310
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 3 cut(s) 218, 231, 385
BslI CCNNNNNNNGG 4 cut(s) 139, 493, 562, 786
BsmAI GTCTC 1 cut(s) 314
BsmBI CGTCTC 1 cut(s) 314
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 81, 310
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 5 cut(s) 97, 446, 475, 535, 703
Bsp68I TCGCGA 1 cut(s) 102
BspACI CCGC 2 cut(s) 556, 730
BspCNI CTCAG 3 cut(s) 22, 181, 736
BspDI ATCGAT 1 cut(s) 281
BspEI TCCGGA 1 cut(s) 217
BspFNI CGCG 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 592
BspPI GGATC 2 cut(s) 454, 530
BspQI GCTCTTC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 203
BsrI ACTGG 3 cut(s) 355, 487, 748
BssECI CCNNGG 2 cut(s) 681, 810
BssMI GATC 5 cut(s) 97, 446, 475, 535, 703
BssT1I CCWWGG 1 cut(s) 681
Bst6I CTCTTC 4 cut(s) 2, 66, 122, 417
BstAPI GCANNNNNTGC 1 cut(s) 587
BstDEI CTNAG 3 cut(s) 30, 168, 723
BstDSI CCRYGG 1 cut(s) 810
BstENI CCTNNNNNAGG 1 cut(s) 137
BstF5I GGATG 2 cut(s) 214, 298
BstFNI CGCG 1 cut(s) 102
BstH2I RGCGCY 2 cut(s) 165, 730
BstHHI GCGC 3 cut(s) 164, 729, 879
BstKTI GATC 5 cut(s) 100, 449, 478, 538, 706
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 5 cut(s) 97, 446, 475, 535, 703
BstMWI GCNNNNNNNGC 2 cut(s) 40, 587
BstSCI CCNGG 2 cut(s) 229, 384
BstSFI CTRYAG 2 cut(s) 439, 588
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 2 cut(s) 52, 574
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 2 cut(s) 446, 703
BstYI RGATCY 2 cut(s) 446, 703
Bsu15I ATCGAT 1 cut(s) 281
BsuTUI ATCGAT 1 cut(s) 281
BtgI CCRYGG 1 cut(s) 810
BtsCI GGATG 2 cut(s) 214, 298
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 3 cut(s) 405, 494, 739
BtuMI TCGCGA 1 cut(s) 102
CaiI CAGNNNCTG 1 cut(s) 370
CfoI GCGC 3 cut(s) 164, 729, 879
Cfr13I GGNCC 1 cut(s) 858
ClaI ATCGAT 1 cut(s) 281
CviAII CATG 2 cut(s) 374, 619
DdeI CTNAG 3 cut(s) 30, 168, 723
DpnI GATC 5 cut(s) 99, 448, 477, 537, 705
DpnII GATC 5 cut(s) 97, 446, 475, 535, 703
Eam1104I CTCTTC 4 cut(s) 2, 66, 122, 417
EarI CTCTTC 4 cut(s) 2, 66, 122, 417
Eco130I CCWWGG 1 cut(s) 681
Eco32I GATATC 2 cut(s) 279, 418
Eco47I GGWCC 1 cut(s) 858
Eco47III AGCGCT 1 cut(s) 163
Eco57I CTGAAG 2 cut(s) 429, 786
Eco88I CYCGRG 1 cut(s) 287
EcoNI CCTNNNNNAGG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 858
EcoRV GATATC 2 cut(s) 279, 418
EcoT14I CCWWGG 1 cut(s) 681
ErhI CCWWGG 1 cut(s) 681
Esp3I CGTCTC 1 cut(s) 314
FaeI CATG 2 cut(s) 377, 622
FatI CATG 2 cut(s) 373, 618
Fnu4HI GCNGC 3 cut(s) 41, 588, 730
FokI GGATG 2 cut(s) 201, 305
Fsp4HI GCNGC 3 cut(s) 41, 588, 730
FspBI CTAG 2 cut(s) 21, 773
GlaI GCGC 3 cut(s) 163, 728, 878
GluI GCNGC 3 cut(s) 41, 588, 730
HaeII RGCGCY 2 cut(s) 165, 730
HapII CCGG 3 cut(s) 218, 231, 385
HhaI GCGC 3 cut(s) 164, 729, 879
Hin1II CATG 2 cut(s) 377, 622
Hin6I GCGC 3 cut(s) 162, 727, 877
HinP1I GCGC 3 cut(s) 162, 727, 877
HinfI GANTC 6 cut(s) 226, 283, 367, 452, 548, 755
HpaII CCGG 3 cut(s) 218, 231, 385
HphI GGTGA 3 cut(s) 245, 286, 365
Hpy188I TCNGA 7 cut(s) 31, 366, 451, 457, 535, 643, 805
Hpy188III TCNNGA 4 cut(s) 101, 218, 572, 780
Hpy99I CGWCG 1 cut(s) 95
HpyCH4V TGCA 6 cut(s) 214, 268, 373, 492, 590, 713
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 587
HpyF3I CTNAG 3 cut(s) 30, 168, 723
Hsp92II CATG 2 cut(s) 377, 622
HspAI GCGC 3 cut(s) 162, 727, 877
Kpn2I TCCGGA 1 cut(s) 217
Kzo9I GATC 5 cut(s) 97, 446, 475, 535, 703
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 2 cut(s) 76, 87
Lsp1109I GCAGC 2 cut(s) 52, 574
LweI GCATC 1 cut(s) 223
MaeI CTAG 2 cut(s) 21, 773
MaeIII GTNAC 1 cut(s) 403
MalI GATC 5 cut(s) 99, 448, 477, 537, 705
MboI GATC 5 cut(s) 97, 446, 475, 535, 703
MfeI CAATTG 1 cut(s) 263
MflI RGATCY 2 cut(s) 446, 703
MhlI GDGCHC 2 cut(s) 81, 310
MluCI AATT 3 cut(s) 197, 263, 821
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 1 cut(s) 668
MspI CCGG 3 cut(s) 218, 231, 385
MspR9I CCNGG 2 cut(s) 231, 386
MunI CAATTG 1 cut(s) 263
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 2 cut(s) 40, 587
NciI CCSGG 2 cut(s) 231, 386
NdeII GATC 5 cut(s) 97, 446, 475, 535, 703
NlaIII CATG 2 cut(s) 377, 622
NmuCI GTSAC 1 cut(s) 403
NruI TCGCGA 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 99
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 6 cut(s) 226, 283, 367, 452, 548, 755
PkrI GCNGC 3 cut(s) 42, 589, 731
PpuMI RGGWCCY 1 cut(s) 858
Psp5II RGGWCCY 1 cut(s) 858
PspPI GGNCC 1 cut(s) 858
PspPPI RGGWCCY 1 cut(s) 858
PstI CTGCAG 1 cut(s) 592
PstNI CAGNNNCTG 1 cut(s) 370
PsuI RGATCY 2 cut(s) 446, 703
RruI TCGCGA 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 668
SatI GCNGC 3 cut(s) 41, 588, 730
Sau3AI GATC 5 cut(s) 97, 446, 475, 535, 703
Sau96I GGNCC 1 cut(s) 858
ScrFI CCNGG 2 cut(s) 231, 386
SduI GDGCHC 2 cut(s) 81, 310
SfaNI GCATC 1 cut(s) 223
SfcI CTRYAG 2 cut(s) 439, 588
SinI GGWCC 1 cut(s) 858
Sse9I AATT 3 cut(s) 197, 263, 821
SsiI CCGC 2 cut(s) 556, 730
SspI AATATT 1 cut(s) 380
SspMI CTAG 2 cut(s) 21, 773
StyD4I CCNGG 2 cut(s) 229, 384
StyI CCWWGG 1 cut(s) 681
TaqI TCGA 6 cut(s) 60, 281, 420, 521, 546, 758
TasI AATT 3 cut(s) 197, 263, 821
TauI GCSGC 1 cut(s) 732
TfiI GAWTC 6 cut(s) 226, 283, 367, 452, 548, 755
Tru1I TTAA 1 cut(s) 668
Tru9I TTAA 1 cut(s) 668
TscAI CASTG 3 cut(s) 412, 494, 739
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 2 cut(s) 40, 587
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 3 cut(s) 390, 518, 635
TspGWI ACGGA 2 cut(s) 100, 827
TspRI CASTG 3 cut(s) 412, 494, 739
VpaK11BI GGWCC 1 cut(s) 858
XagI CCTNNNNNAGG 1 cut(s) 137
XapI RAATTY 1 cut(s) 821
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 2 cut(s) 21, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.