pycom14g06610

Serine hydrolase involved in the detoxification of formaldehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012329
Physical Location & Seq
Forward (+)
6556036 .. 6560141
4106 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
ATGGAGACGAAGCCGAGTGAGGTGAGCAGCTCGAAGATGTTCGGAGGGTACAACAAGAGGTACAAGCATTTCAGCCCTACGCTCGGCTGCTCCATGACCTTCCACATCTACTTCCCTCCTTCTCCTTCCCCTTCCCACCGATTCCCTGTACTTTACTGGCTCTCTGGCCTGACCTGCACAGATGAAAATTTTATAACCAAGTCAGGAGCTCAACGTGTTGCCTCAAGTGAGGGTGTTGCTCTGATCGCCCCTGATACCTCTCCAAGAGGCTTGTCTATAGAAGGAGAGGCGGATAGCTGGGATTTTGGTGTAGGTGCTGGGTTTTATCTCAATGCTACTGAAGAGAAATGGAAGAACTGGCGCATGTATGATTATGTTGTCAAGGAGCTGCCACAACTTCTGAATGAAAACTTTTCACAGCTTGATACATCACGGGCATCTATATCTGGTCATTCTATGGGTGGGCACGGTGCTTTGACAATCTACCTGAAAAACCTGGATAAATATAAGTCAGTATCTGCCTTTGCACCCATTGTGAATCCTACTAACTGTCCATGGGGCCAGAAAGCTTTCTCAAATTATCTAGGTGGCAATAAAACCGATTGGGAAGAATATGATGCCACTTCCCTAATTAAGAAGTTTAACGATGTGTCAGCAGCTACCATTTTAATTGATCAGGGGGAAGATGATAAATTCTTGCATGATCGGTTAATGCCACACAAGTTTGAAGAGGCATGTCGGAGTGCTAAAGTTCCACTCCTTTTGCGACTGCAGCCCGGTTATGATCACTCATACTTTTTCATTTCCACCTTCATCGACGATCACATCCATCACCATGCTCAAGCTCTTAATCTACCGTGA

Protein Analysis

287

Amino Acids

32.16

Weight (kDa)

6.17

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Esterase PF00756 25 - 277 7.8e-63 Putative esterase
Peptidase_S9 PF00326 109 - 265 1.6e-09 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012329)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41530
fragaria_vesca FvH4_6g24520
malus_domestica MD12G1082500.v1.1 MD14G1077300.v1.1
prunus_persica Prupe.7G053400_v2.0.a1 Prupe.7G053400_v2.0.a1
pyrus_communis pycom12g06870 pycom14g06610
rosa_chinensis RchiOBHm_Chr3g0483631
rosa_laevigata RLG00000023263
rosa_multiflora Rmu_co8515891.1_g000001 Rmu_sc0000555.1_g000009
rosa_roxburghii Rroxscaffold_6G00398170
rosa_rugosa Rorug03G0207500
rosa_samantha Rh3AG256000 Rh3BG292700 Rh3CG290400 Rh3DG286300
rosa_wichuraiana Rw3G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 182
AciI CCGC 1 cut(s) 290
AcsI RAATTY 2 cut(s) 187, 692
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 3 cut(s) 50, 62, 150
AfiI CCNNNNNNNGG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 1 cut(s) 728
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 8 cut(s) 30, 209, 297, 388, 421, 569, 659, 845
AluI AGCT 8 cut(s) 30, 209, 297, 388, 421, 569, 659, 845
Alw21I GWGCWC 1 cut(s) 211
AlwNI CAGNNNCTG 1 cut(s) 518
AoxI GGCC 2 cut(s) 166, 559
ApeKI GCWGC 5 cut(s) 27, 87, 388, 656, 772
ApoI RAATTY 2 cut(s) 187, 692
Asp700I GAANNNNTTC 2 cut(s) 38, 569
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 559
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 2 cut(s) 34, 824
BaeGI GKGCMC 1 cut(s) 468
BanII GRGCYC 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 5 cut(s) 39, 74, 375, 668, 784
BccI CCATC 1 cut(s) 837
BciT130I CCWGG 1 cut(s) 497
BclI TGATCA 2 cut(s) 673, 784
BcnI CCSGG 1 cut(s) 777
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 2 cut(s) 276, 770
BfuAI ACCTGC 1 cut(s) 182
BisI GCNGC 5 cut(s) 28, 88, 389, 657, 773
BlsI GCNGC 5 cut(s) 29, 89, 390, 658, 774
Bme1390I CCNGG 2 cut(s) 497, 777
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 1 cut(s) 560
BmrFI CCNGG 2 cut(s) 497, 777
BmsI GCATC 2 cut(s) 446, 607
BpuEI CTTGAG 2 cut(s) 208, 825
BpuMI CCSGG 1 cut(s) 777
BsaJI CCNNGG 1 cut(s) 554
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 161, 362
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 1 cut(s) 554
BseGI GGATG 1 cut(s) 825
BseLI CCNNNNNNNGG 1 cut(s) 83
BseNI ACTGG 2 cut(s) 161, 362
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 5 cut(s) 39, 74, 375, 668, 784
BseYI CCCAGC 2 cut(s) 297, 317
BsgI GTGCAG 1 cut(s) 160
BshFI GGCC 2 cut(s) 168, 561
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 1 cut(s) 777
BslI CCNNNNNNNGG 1 cut(s) 83
BsnI GGCC 2 cut(s) 168, 561
Bsp1286I GDGCHC 2 cut(s) 211, 468
Bsp143I GATC 5 cut(s) 243, 673, 703, 784, 820
Bsp19I CCATGG 1 cut(s) 554
BspACI CCGC 1 cut(s) 290
BspANI GGCC 2 cut(s) 168, 561
BspLI GGNNCC 1 cut(s) 560
BspMAI CTGCAG 1 cut(s) 774
BspMI ACCTGC 1 cut(s) 182
BsrI ACTGG 2 cut(s) 161, 362
BssECI CCNNGG 1 cut(s) 554
BssMI GATC 5 cut(s) 243, 673, 703, 784, 820
BssT1I CCWWGG 1 cut(s) 554
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 3 cut(s) 470, 551, 858
Bst6I CTCTTC 2 cut(s) 336, 723
BstDSI CCRYGG 1 cut(s) 554
BstF5I GGATG 1 cut(s) 825
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 5 cut(s) 246, 676, 706, 787, 823
BstMBI GATC 5 cut(s) 243, 673, 703, 784, 820
BstMWI GCNNNNNNNGC 3 cut(s) 174, 245, 772
BstNI CCWGG 1 cut(s) 497
BstNSI RCATGY 2 cut(s) 367, 738
BstSCI CCNGG 2 cut(s) 495, 775
BstSFI CTRYAG 2 cut(s) 276, 770
BstSLI GKGCMC 1 cut(s) 468
BstV1I GCAGC 5 cut(s) 39, 74, 375, 668, 784
BsuRI GGCC 2 cut(s) 168, 561
BtgI CCRYGG 1 cut(s) 554
BtsCI GGATG 1 cut(s) 825
BveI ACCTGC 1 cut(s) 182
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 1 cut(s) 363
Cfr13I GGNCC 1 cut(s) 559
Csp6I GTAC 3 cut(s) 49, 61, 149
CspCI CAANNNNNGTGG 2 cut(s) 744, 779
CviAII CATG 6 cut(s) 94, 364, 555, 701, 735, 836
CviQI GTAC 3 cut(s) 49, 61, 149
DpnI GATC 5 cut(s) 245, 675, 705, 786, 822
DpnII GATC 5 cut(s) 243, 673, 703, 784, 820
Eam1104I CTCTTC 2 cut(s) 336, 723
EarI CTCTTC 2 cut(s) 336, 723
EciI GGCGGA 1 cut(s) 305
Ecl136II GAGCTC 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 554
Eco24I GRGCYC 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 495
EcoT14I CCWWGG 1 cut(s) 554
EcoT38I GRGCYC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 554
FaeI CATG 6 cut(s) 97, 367, 558, 704, 738, 839
FatI CATG 6 cut(s) 93, 363, 554, 700, 734, 835
FbaI TGATCA 2 cut(s) 673, 784
Fnu4HI GCNGC 5 cut(s) 28, 88, 389, 657, 773
FokI GGATG 1 cut(s) 812
FriOI GRGCYC 1 cut(s) 211
Fsp4HI GCNGC 5 cut(s) 28, 88, 389, 657, 773
FspBI CTAG 1 cut(s) 584
GlaI GCGC 1 cut(s) 362
GluI GCNGC 5 cut(s) 28, 88, 389, 657, 773
GsaI CCCAGC 2 cut(s) 301, 321
HaeIII GGCC 2 cut(s) 168, 561
HapII CCGG 1 cut(s) 777
HhaI GCGC 1 cut(s) 363
Hin1II CATG 6 cut(s) 97, 367, 558, 704, 738, 839
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 2 cut(s) 141, 538
HpaII CCGG 1 cut(s) 777
HphI GGTGA 2 cut(s) 34, 824
Hpy188I TCNGA 4 cut(s) 44, 243, 402, 741
Hpy188III TCNNGA 1 cut(s) 204
Hpy99I CGWCG 1 cut(s) 821
HpyAV CCTTC 6 cut(s) 109, 129, 135, 141, 275, 820
HpyCH4III ACNGT 3 cut(s) 470, 551, 858
HpyCH4IV ACGT 1 cut(s) 214
HpyCH4V TGCA 4 cut(s) 177, 527, 700, 772
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 245, 772
HpySE526I ACGT 1 cut(s) 214
Hsp92II CATG 6 cut(s) 97, 367, 558, 704, 738, 839
HspAI GCGC 1 cut(s) 361
Ksp22I TGATCA 2 cut(s) 673, 784
Kzo9I GATC 5 cut(s) 243, 673, 703, 784, 820
LmnI GCTCC 3 cut(s) 95, 206, 385
Lsp1109I GCAGC 5 cut(s) 39, 74, 375, 668, 784
LweI GCATC 2 cut(s) 446, 607
MaeI CTAG 1 cut(s) 584
MaeII ACGT 1 cut(s) 214
MalI GATC 5 cut(s) 245, 675, 705, 786, 822
MboI GATC 5 cut(s) 243, 673, 703, 784, 820
MboII GAAGA 6 cut(s) 46, 353, 364, 620, 695, 740
MhlI GDGCHC 2 cut(s) 211, 468
MluCI AATT 5 cut(s) 187, 577, 630, 669, 692
MmeI TCCRAC 1 cut(s) 719
MroXI GAANNNNTTC 2 cut(s) 38, 569
MseI TTAA 5 cut(s) 633, 642, 668, 710, 849
MslI CAYNNNNRTG 1 cut(s) 834
MspI CCGG 1 cut(s) 777
MspR9I CCNGG 2 cut(s) 497, 777
MvaI CCWGG 1 cut(s) 497
MwoI GCNNNNNNNGC 3 cut(s) 174, 245, 772
NciI CCSGG 1 cut(s) 777
NcoI CCATGG 1 cut(s) 554
NdeII GATC 5 cut(s) 243, 673, 703, 784, 820
NlaIII CATG 6 cut(s) 97, 367, 558, 704, 738, 839
NlaIV GGNNCC 1 cut(s) 560
NmeAIII GCCGAG 2 cut(s) 39, 63
NspI RCATGY 2 cut(s) 367, 738
PdmI GAANNNNTTC 2 cut(s) 38, 569
PfeI GAWTC 2 cut(s) 141, 538
PkrI GCNGC 5 cut(s) 29, 89, 390, 658, 774
PsiI TTATAA 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 495
PspFI CCCAGC 2 cut(s) 297, 317
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 1 cut(s) 560
PspPI GGNCC 1 cut(s) 559
PstI CTGCAG 1 cut(s) 774
PstNI CAGNNNCTG 1 cut(s) 518
RsaI GTAC 3 cut(s) 50, 62, 150
RsaNI GTAC 3 cut(s) 49, 61, 149
RseI CAYNNNNRTG 1 cut(s) 834
SacI GAGCTC 1 cut(s) 211
SaqAI TTAA 5 cut(s) 633, 642, 668, 710, 849
SatI GCNGC 5 cut(s) 28, 88, 389, 657, 773
Sau3AI GATC 5 cut(s) 243, 673, 703, 784, 820
Sau96I GGNCC 1 cut(s) 559
ScrFI CCNGG 2 cut(s) 497, 777
SduI GDGCHC 2 cut(s) 211, 468
SfaNI GCATC 2 cut(s) 446, 607
SfcI CTRYAG 2 cut(s) 276, 770
SmiMI CAYNNNNRTG 1 cut(s) 834
SmlI CTYRAG 2 cut(s) 223, 840
SmoI CTYRAG 2 cut(s) 223, 840
Sse9I AATT 5 cut(s) 187, 577, 630, 669, 692
SsiI CCGC 1 cut(s) 290
SspMI CTAG 1 cut(s) 584
SstI GAGCTC 1 cut(s) 211
StyD4I CCNGG 2 cut(s) 495, 775
StyI CCWWGG 1 cut(s) 554
TaaI ACNGT 3 cut(s) 470, 551, 858
TaiI ACGT 1 cut(s) 217
TaqI TCGA 2 cut(s) 32, 816
TasI AATT 5 cut(s) 187, 577, 630, 669, 692
TatI WGTACW 1 cut(s) 148
TfiI GAWTC 2 cut(s) 141, 538
Tru1I TTAA 5 cut(s) 633, 642, 668, 710, 849
Tru9I TTAA 5 cut(s) 633, 642, 668, 710, 849
TseI GCWGC 5 cut(s) 27, 87, 388, 656, 772
TspDTI ATGAA 4 cut(s) 198, 420, 790, 802
XapI RAATTY 2 cut(s) 187, 692
XceI RCATGY 2 cut(s) 367, 738
XmnI GAANNNNTTC 2 cut(s) 38, 569
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.