pycom14g14620

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013667
Physical Location & Seq
Reverse (-)
17359885 .. 17362507
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1800 bp
ATGGAAAATCAACCCTTACTATTGGGCTTTTTCCCCAAAAAAAAAAAAAACTTTACTATTTGGGCTTTTACCCACCCGCAATCCAACCCAAAAAGAAAAAGGAAACATATCAAGAACAGTGGAGGAATCAGCCTCTGCGGCTCCGCTTCAAACGCTGGTCAATTTTCCTTCGGCAGCTCCGTCGCGGCGCTAACCCCAACACAATCAGCTGTTTGGAAGCTCAGAAAACATGACGGAAAGCACCGGAAAATCGCAGAGAAAAATCCGAGCACGATGAAGACGACGGAGTTCATGGACAAGCAGATCATGGACCTCTCTCGATCCCACTCCGACGATTTCTATCAGCTTTCGTACCCTCAACAATTCGATGACGACGACAAGGACGACGAAGATTTCGTTTCCAGCTTTCAGTTCCACCCCGGACGACGGGTCGTCTCGCAGGAGCCGCGTATTTCCGCTCTCGAGCGTGGCAGTTCTGTGGACTTCCTCAACCTTGCAAAATCCGGAAGCAAGCATGTTGACAGGGCTTCCGAGGATTCAGCATTGATTTCGGTGATAGAACGAAAGATGGACGAGCATTTTGGCAATCTGTTGCACGGGGTGGAGGGTCTCAGTGCGCGAATTTCTCAGCTGGAGACTCGATTACGCCGGCTGGAGAATTCTGTTGGCGACTTGAAGGATTCTACTGAAATCAACCATGGGAAGGCTGATGGGAAACTGAGGCAACTAGAGAATATGCTAAGAGAGGTTGACGGTGGTATGCAGGATTTGAGAGATAAACAAGAGATAACAGAGGCTCAGTTGGAGCTTGCAAAGCTTCAAATGTTAAAGGGTTACCAGCAATCGGAAAACCAAACGAACAATGTACAAACAGTTTCAGCTCCAGGAGTACTGTCCTCGACGCCTCAGCAAAGCTACCAACCGCATTTAGTTCAGGGTCCTACCCAACAAGTTCCTTCGCTTCCTACTGATGATCCCCAAACTCTATCCCATCAGAATTTTCCACCAGCACAGGTCTCCACCCAGTCACCTGTGCAAAATCCACAACCCACACCTCAGATGTACTACAGCTTTCAAGTACAGCAGTCTCAACTGCCGCCCACTGCACCGTATCATCTTTCTCCTCCAGCACCACATCCTTCACTAACCTCACAATTACAGCAGCTGCCTCAACAGCACGCTCCTTTCAATGCTGTTGACCCACAAGCCCAGTTTTCTTTGGTTCATAATCCCGGAGAAACCTCCACGCCATCTCAAAGATATCCTCCAAGCTTCCACAAGACCTCTAATGCACCTGGCATGGCTCTCCCAACCCAACAACAGCAATATGCGGGTTCCAGTCAGTATACGCATGATCAAACCGCAAGCAATCAGTACCCGGAGTTTTCCCCTGGCCATATGCAGCCAGTTCAACATTCATGTTTTGATGATTTTCGTTCCCACGGTGGATCTCCATCTGACAACAGCGGTCCAAACATGAAACACTCACTAGTCTTGCCAGCCTCATTAGACCGTTCTGGTGGAAGCAGCTTTTCGAAACTGCCAACTGCCAAGGTTTTGCCACATGCAATACCCATGGCTGCTAGTGTGGATAAGGAATCGAGTTCCAGTGGGACTGGAAACAGAGTACCAATTGATAATGTCATTGATAAGGTTGTAGCGATGGGATTCCGAAGGGACATGGTCAGAGCAACAGTGAGGAAAATGACTGAGAATGGGCAGTCGGTGGACGTCAATCTTGTGCTGGACAAGCTGATGAACAATGGAGAACAGCCTCAAAGTGGTGGATTTGGCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

600

Amino Acids

66.2

Weight (kDa)

7.4

Isoelectric Point (pI)

53.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 545 - 589 3.3e-19 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05870 FvH4_5g05870
malus_domestica MD06G1169700.v1.1 MD14G1175200.v1.1
prunus_persica Prupe.5G168500_v2.0.a1
pyrus_communis pycom06g15100 pycom14g14620
rosa_chinensis RchiOBHm_Chr7g0192951
rosa_laevigata RLG00000004309
rosa_multiflora Rmu_sc0002040.1_g000061
rosa_roxburghii Rroxscaffold_3G00262510
rosa_rugosa Rorug07G0006400 Rorug07G0294800
rosa_samantha Rh7AG131600 Rh7BG132500 Rh7CG135700 Rh7DG133600
rosa_wichuraiana Rw7G011230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1734
AccBSI CCGCTC 1 cut(s) 458
AccI GTMKAC 1 cut(s) 1346
AccII CGCG 3 cut(s) 185, 448, 619
AccIII TCCGGA 1 cut(s) 503
AclWI GGATC 3 cut(s) 315, 968, 1456
AcoI YGGCCR 2 cut(s) 1393, 1792
AcsI RAATTY 3 cut(s) 621, 658, 997
AcyI GRCGYC 2 cut(s) 902, 1731
AdeI CACNNNGTG 1 cut(s) 601
AfaI GTAC 7 cut(s) 353, 867, 891, 1064, 1080, 1376, 1629
AfiI CCNNNNNNNGG 4 cut(s) 426, 703, 844, 1781
AgsI TTSAA 6 cut(s) 150, 676, 821, 1076, 1189, 1412
AhdI GACNNNNNGTC 2 cut(s) 428, 431
AhlI ACTAGT 1 cut(s) 1489
AjnI CCWGG 3 cut(s) 883, 1294, 1390
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 5 cut(s) 439, 614, 629, 1021, 1092
AlwI GGATC 3 cut(s) 315, 968, 1456
AlwNI CAGNNNCTG 2 cut(s) 135, 1165
Ama87I CYCGRG 1 cut(s) 461
Aor13HI TCCGGA 1 cut(s) 503
AoxI GGCC 2 cut(s) 1393, 1792
ApeKI GCWGC 6 cut(s) 174, 1162, 1165, 1402, 1527, 1580
ApoI RAATTY 3 cut(s) 621, 658, 997
ArsI GACNNNNNNTTYG 4 cut(s) 377, 409, 563, 595
AspLEI GCGC 2 cut(s) 190, 619
AspS9I GGNCC 3 cut(s) 310, 938, 1469
AsuC2I CCSGG 3 cut(s) 420, 1233, 1379
AsuHPI GGTGA 2 cut(s) 565, 1020
AsuII TTCGAA 1 cut(s) 1535
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 3 cut(s) 310, 938, 1469
BalI TGGCCA 1 cut(s) 1395
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 272
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 6 cut(s) 186, 1152, 1174, 1414, 1539, 1567
BccI CCATC 6 cut(s) 562, 704, 999, 1258, 1462, 1657
BcgI CGANNNNNNTGC 4 cut(s) 163, 197, 531, 565
BciT130I CCWGG 3 cut(s) 885, 1296, 1392
BclI TGATCA 1 cut(s) 1354
BcnI CCSGG 3 cut(s) 420, 1233, 1379
BcoDI GTCTC 5 cut(s) 439, 614, 629, 1021, 1092
BcuI ACTAGT 1 cut(s) 1489
BfaI CTAG 3 cut(s) 728, 1490, 1584
BfmI CTRYAG 1 cut(s) 1066
BfoI RGCGCY 1 cut(s) 191
BglI GCCNNNNNGGC 1 cut(s) 138
BmcAI AGTACT 1 cut(s) 891
Bme1390I CCNGG 6 cut(s) 420, 885, 1233, 1296, 1379, 1392
Bme18I GGWCC 3 cut(s) 310, 938, 1469
BmeRI GACNNNNNGTC 2 cut(s) 428, 431
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 3 cut(s) 310, 938, 1469
BmiI GGNNCC 4 cut(s) 142, 444, 939, 1336
BmrFI CCNGG 6 cut(s) 420, 885, 1233, 1296, 1379, 1392
BmrI ACTGGG 2 cut(s) 1018, 1204
BmuI ACTGGG 2 cut(s) 1018, 1204
BpiI GAAGAC 1 cut(s) 284
BpmI CTGGAG 4 cut(s) 653, 674, 867, 1110
Bpu10I CCTNAGC 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 1535
BpuMI CCSGG 3 cut(s) 420, 1233, 1379
BsaBI GATNNNNATC 2 cut(s) 339, 1453
BsaHI GRCGYC 2 cut(s) 902, 1731
BsaI GGTCTC 2 cut(s) 614, 1021
BsaJI CCNNGG 7 cut(s) 418, 531, 697, 1390, 1441, 1551, 1575
BsaWI WCCGGW 2 cut(s) 243, 503
Bsc4I CCNNNNNNNGG 4 cut(s) 426, 703, 844, 1781
Bse118I RCCGGY 2 cut(s) 648, 1794
Bse1I ACTGG 6 cut(s) 1024, 1210, 1338, 1406, 1608, 1621
Bse8I GATNNNNATC 2 cut(s) 339, 1453
BseAI TCCGGA 1 cut(s) 503
BseBI CCWGG 3 cut(s) 885, 1296, 1392
BseDI CCNNGG 7 cut(s) 418, 531, 697, 1390, 1441, 1551, 1575
BseGI GGATG 1 cut(s) 1135
BseJI GATNNNNATC 2 cut(s) 339, 1453
BseLI CCNNNNNNNGG 4 cut(s) 426, 703, 844, 1781
BseMII CTCAG 8 cut(s) 235, 625, 641, 710, 812, 920, 1070, 1701
BseNI ACTGG 6 cut(s) 1024, 1210, 1338, 1406, 1608, 1621
BseRI GAGGAG 1 cut(s) 1113
BseXI GCAGC 6 cut(s) 186, 1152, 1174, 1414, 1539, 1567
BsgI GTGCAG 1 cut(s) 1089
Bsh1236I CGCG 3 cut(s) 185, 448, 619
BshFI GGCC 2 cut(s) 1395, 1794
BsiHKAI GWGCWC 1 cut(s) 272
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 7 cut(s) 244, 420, 504, 649, 1233, 1379, 1795
BslFI GGGAC 2 cut(s) 1627, 1691
BslI CCNNNNNNNGG 4 cut(s) 426, 703, 844, 1781
BsmAI GTCTC 5 cut(s) 439, 614, 629, 1021, 1092
BsmBI CGTCTC 1 cut(s) 439
BsmFI GGGAC 2 cut(s) 1627, 1691
BsnI GGCC 2 cut(s) 1395, 1794
Bso31I GGTCTC 2 cut(s) 614, 1021
BsoBI CYCGRG 1 cut(s) 461
Bsp119I TTCGAA 1 cut(s) 1535
Bsp1286I GDGCHC 1 cut(s) 272
Bsp13I TCCGGA 1 cut(s) 503
Bsp1407I TGTACA 1 cut(s) 865
Bsp143I GATC 5 cut(s) 303, 320, 973, 1354, 1448
Bsp19I CCATGG 2 cut(s) 697, 1575
BspANI GGCC 2 cut(s) 1395, 1794
BspCNI CTCAG 8 cut(s) 234, 624, 640, 711, 811, 919, 1069, 1702
BspEI TCCGGA 1 cut(s) 503
BspFNI CGCG 3 cut(s) 185, 448, 619
BspLI GGNNCC 4 cut(s) 142, 444, 939, 1336
BspPI GGATC 3 cut(s) 315, 968, 1456
BspT104I TTCGAA 1 cut(s) 1535
BspTNI GGTCTC 2 cut(s) 614, 1021
BsrBI CCGCTC 1 cut(s) 458
BsrFI RCCGGY 2 cut(s) 648, 1794
BsrGI TGTACA 1 cut(s) 865
BsrI ACTGG 6 cut(s) 1024, 1210, 1338, 1406, 1608, 1621
BssAI RCCGGY 2 cut(s) 648, 1794
BssECI CCNNGG 7 cut(s) 418, 531, 697, 1390, 1441, 1551, 1575
BssMI GATC 5 cut(s) 303, 320, 973, 1354, 1448
BssNAI GTATAC 1 cut(s) 1347
BssNI GRCGYC 2 cut(s) 902, 1731
BssT1I CCWWGG 3 cut(s) 697, 1551, 1575
Bst1107I GTATAC 1 cut(s) 1347
Bst2UI CCWGG 3 cut(s) 885, 1296, 1392
Bst4CI ACNGT 8 cut(s) 119, 755, 874, 894, 1110, 1445, 1514, 1696
BstACI GRCGYC 2 cut(s) 902, 1731
BstAUI TGTACA 1 cut(s) 865
BstBI TTCGAA 1 cut(s) 1535
BstC8I GCNNGC 6 cut(s) 512, 650, 810, 1179, 1366, 1500
BstDEI CTNAG 9 cut(s) 221, 611, 627, 719, 740, 798, 906, 1056, 1710
BstDSI CCRYGG 3 cut(s) 697, 1441, 1575
BstEII GGTNACC 1 cut(s) 833
BstF5I GGATG 1 cut(s) 1135
BstFNI CGCG 3 cut(s) 185, 448, 619
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 2 cut(s) 190, 619
BstKTI GATC 5 cut(s) 306, 323, 976, 1357, 1451
BstMAI GTCTC 5 cut(s) 439, 614, 629, 1021, 1092
BstMBI GATC 5 cut(s) 303, 320, 973, 1354, 1448
BstMWI GCNNNNNNNGC 6 cut(s) 138, 152, 445, 814, 1174, 1750
BstNI CCWGG 3 cut(s) 885, 1296, 1392
BstNSI RCATGY 2 cut(s) 518, 1568
BstPI GGTNACC 1 cut(s) 833
BstSCI CCNGG 6 cut(s) 418, 883, 1231, 1294, 1377, 1390
BstSFI CTRYAG 1 cut(s) 1066
BstUI CGCG 3 cut(s) 185, 448, 619
BstV1I GCAGC 6 cut(s) 186, 1152, 1174, 1414, 1539, 1567
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 1448
BstYI RGATCY 1 cut(s) 1448
BstZ17I GTATAC 1 cut(s) 1347
BsuRI GGCC 2 cut(s) 1395, 1794
BtgI CCRYGG 3 cut(s) 697, 1441, 1575
BtgZI GCGATG 1 cut(s) 1676
BtsCI GGATG 1 cut(s) 1135
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 5 cut(s) 124, 619, 1101, 1615, 1701
Cac8I GCNNGC 6 cut(s) 512, 650, 810, 1179, 1366, 1500
CaiI CAGNNNCTG 2 cut(s) 135, 1165
CfoI GCGC 2 cut(s) 190, 619
Cfr10I RCCGGY 2 cut(s) 648, 1794
Cfr13I GGNCC 3 cut(s) 310, 938, 1469
CseI GACGC 1 cut(s) 910
Csp6I GTAC 7 cut(s) 352, 866, 890, 1063, 1079, 1375, 1628
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviQI GTAC 7 cut(s) 352, 866, 890, 1063, 1079, 1375, 1628
DdeI CTNAG 9 cut(s) 221, 611, 627, 719, 740, 798, 906, 1056, 1710
DpnI GATC 5 cut(s) 305, 322, 975, 1356, 1450
DpnII GATC 5 cut(s) 303, 320, 973, 1354, 1448
DraIII CACNNNGTG 1 cut(s) 601
DriI GACNNNNNGTC 2 cut(s) 428, 431
EaeI YGGCCR 2 cut(s) 1393, 1792
Eam1105I GACNNNNNGTC 2 cut(s) 428, 431
Eco130I CCWWGG 3 cut(s) 697, 1551, 1575
Eco31I GGTCTC 2 cut(s) 614, 1021
Eco32I GATATC 1 cut(s) 1262
Eco47I GGWCC 3 cut(s) 310, 938, 1469
Eco88I CYCGRG 1 cut(s) 461
Eco91I GGTNACC 1 cut(s) 833
EcoO109I RGGNCCY 1 cut(s) 938
EcoO65I GGTNACC 1 cut(s) 833
EcoRI GAATTC 1 cut(s) 658
EcoRII CCWGG 3 cut(s) 883, 1294, 1390
EcoRV GATATC 1 cut(s) 1262
EcoT14I CCWWGG 3 cut(s) 697, 1551, 1575
ErhI CCWWGG 3 cut(s) 697, 1551, 1575
Esp3I CGTCTC 1 cut(s) 439
FaqI GGGAC 2 cut(s) 1627, 1691
FauI CCCGC 2 cut(s) 84, 1324
FauNDI CATATG 1 cut(s) 1398
FbaI TGATCA 1 cut(s) 1354
FblI GTMKAC 1 cut(s) 1346
FokI GGATG 1 cut(s) 1122
FspBI CTAG 3 cut(s) 728, 1490, 1584
GlaI GCGC 2 cut(s) 189, 618
GsuI CTGGAG 4 cut(s) 653, 674, 867, 1110
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 2 cut(s) 1395, 1794
HapII CCGG 7 cut(s) 244, 420, 504, 649, 1233, 1379, 1795
HgaI GACGC 1 cut(s) 910
HhaI GCGC 2 cut(s) 190, 619
Hin1I GRCGYC 2 cut(s) 902, 1731
Hin6I GCGC 2 cut(s) 188, 617
HinP1I GCGC 2 cut(s) 188, 617
HincII GTYRAC 3 cut(s) 520, 751, 1198
HindII GTYRAC 3 cut(s) 520, 751, 1198
HindIII AAGCTT 2 cut(s) 815, 1270
HinfI GANTC 6 cut(s) 126, 536, 637, 680, 1598, 1668
HpaII CCGG 7 cut(s) 244, 420, 504, 649, 1233, 1379, 1795
HphI GGTGA 2 cut(s) 565, 1020
Hpy166II GTNNAC 6 cut(s) 481, 520, 751, 1198, 1347, 1729
Hpy188III TCNNGA 4 cut(s) 112, 318, 461, 504
Hpy8I GTNNAC 6 cut(s) 481, 520, 751, 1198, 1347, 1729
Hpy99I CGWCG 7 cut(s) 185, 286, 335, 377, 389, 429, 904
HpyAV CCTTC 6 cut(s) 178, 670, 697, 966, 1149, 1668
HpyCH4III ACNGT 8 cut(s) 119, 755, 874, 894, 1110, 1445, 1514, 1696
HpyCH4IV ACGT 1 cut(s) 1731
HpyCH4V TGCA 9 cut(s) 497, 595, 763, 812, 1036, 1106, 1292, 1402, 1568
HpyF10VI GCNNNNNNNGC 6 cut(s) 138, 152, 445, 814, 1174, 1750
HpyF3I CTNAG 9 cut(s) 221, 611, 627, 719, 740, 798, 906, 1056, 1710
HpySE526I ACGT 1 cut(s) 1731
Hsp92I GRCGYC 2 cut(s) 902, 1731
HspAI GCGC 2 cut(s) 188, 617
Kpn2I TCCGGA 1 cut(s) 503
KroI GCCGGC 1 cut(s) 648
KroNI GCCGGC 1 cut(s) 650
Ksp22I TGATCA 1 cut(s) 1354
Kzo9I GATC 5 cut(s) 303, 320, 973, 1354, 1448
LmnI GCTCC 6 cut(s) 146, 182, 442, 805, 886, 1186
Lsp1109I GCAGC 6 cut(s) 186, 1152, 1174, 1414, 1539, 1567
MaeI CTAG 3 cut(s) 728, 1490, 1584
MaeII ACGT 1 cut(s) 1731
MaeIII GTNAC 2 cut(s) 833, 1026
MalI GATC 5 cut(s) 305, 322, 975, 1356, 1450
MbiI CCGCTC 1 cut(s) 458
MboI GATC 5 cut(s) 303, 320, 973, 1354, 1448
MboII GAAGA 2 cut(s) 289, 401
MfeI CAATTG 1 cut(s) 1632
MflI RGATCY 1 cut(s) 1448
MhlI GDGCHC 1 cut(s) 272
MlsI TGGCCA 1 cut(s) 1395
MluCI AATT 7 cut(s) 161, 362, 621, 658, 997, 1154, 1632
MluNI TGGCCA 1 cut(s) 1395
MlyI GAGTC 1 cut(s) 631
MmeI TCCRAC 3 cut(s) 108, 354, 783
Mox20I TGGCCA 1 cut(s) 1395
MroI TCCGGA 1 cut(s) 503
MroNI GCCGGC 1 cut(s) 648
MscI TGGCCA 1 cut(s) 1395
MseI TTAA 1 cut(s) 827
Msp20I TGGCCA 1 cut(s) 1395
MspA1I CMGCKG 4 cut(s) 209, 631, 1165, 1467
MspI CCGG 7 cut(s) 244, 420, 504, 649, 1233, 1379, 1795
MspR9I CCNGG 6 cut(s) 420, 885, 1233, 1296, 1379, 1392
MunI CAATTG 1 cut(s) 1632
MvaI CCWGG 3 cut(s) 885, 1296, 1392
MvnI CGCG 3 cut(s) 185, 448, 619
MwoI GCNNNNNNNGC 6 cut(s) 138, 152, 445, 814, 1174, 1750
NaeI GCCGGC 1 cut(s) 650
NciI CCSGG 3 cut(s) 420, 1233, 1379
NcoI CCATGG 2 cut(s) 697, 1575
NdeI CATATG 1 cut(s) 1398
NdeII GATC 5 cut(s) 303, 320, 973, 1354, 1448
NgoMIV GCCGGC 1 cut(s) 648
NlaIV GGNNCC 4 cut(s) 142, 444, 939, 1336
NmuCI GTSAC 1 cut(s) 1026
NspI RCATGY 2 cut(s) 518, 1568
NspV TTCGAA 1 cut(s) 1535
PaeR7I CTCGAG 1 cut(s) 461
PcsI WCGNNNNNNNCGW 5 cut(s) 177, 278, 372, 381, 393
PdiI GCCGGC 1 cut(s) 650
PfeI GAWTC 5 cut(s) 126, 536, 680, 1598, 1668
PflFI GACNNNGTC 1 cut(s) 1682
PfoI TCCNGGA 2 cut(s) 883, 1231
PleI GAGTC 1 cut(s) 631
PpsI GAGTC 1 cut(s) 631
PpuMI RGGWCCY 1 cut(s) 938
Psp5II RGGWCCY 1 cut(s) 938
Psp6I CCWGG 3 cut(s) 883, 1294, 1390
PspEI GGTNACC 1 cut(s) 833
PspGI CCWGG 3 cut(s) 883, 1294, 1390
PspN4I GGNNCC 4 cut(s) 142, 444, 939, 1336
PspPI GGNCC 3 cut(s) 310, 938, 1469
PspPPI RGGWCCY 1 cut(s) 938
PstNI CAGNNNCTG 2 cut(s) 135, 1165
PsuI RGATCY 1 cut(s) 1448
PsyI GACNNNGTC 1 cut(s) 1682
PvuII CAGCTG 3 cut(s) 209, 631, 1165
RsaI GTAC 7 cut(s) 353, 867, 891, 1064, 1080, 1376, 1629
RsaNI GTAC 7 cut(s) 352, 866, 890, 1063, 1079, 1375, 1628
SaqAI TTAA 1 cut(s) 827
Sau3AI GATC 5 cut(s) 303, 320, 973, 1354, 1448
Sau96I GGNCC 3 cut(s) 310, 938, 1469
ScaI AGTACT 1 cut(s) 891
SchI GAGTC 1 cut(s) 631
ScrFI CCNGG 6 cut(s) 420, 885, 1233, 1296, 1379, 1392
SduI GDGCHC 1 cut(s) 272
SfcI CTRYAG 1 cut(s) 1066
Sfr274I CTCGAG 1 cut(s) 461
SfuI TTCGAA 1 cut(s) 1535
SinI GGWCC 3 cut(s) 310, 938, 1469
SlaI CTCGAG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
SpeI ACTAGT 1 cut(s) 1489
Sse9I AATT 7 cut(s) 161, 362, 621, 658, 997, 1154, 1632
SspMI CTAG 3 cut(s) 728, 1490, 1584
StyD4I CCNGG 6 cut(s) 418, 883, 1231, 1294, 1377, 1390
StyI CCWWGG 3 cut(s) 697, 1551, 1575
TaaI ACNGT 8 cut(s) 119, 755, 874, 894, 1110, 1445, 1514, 1696
TaiI ACGT 1 cut(s) 1734
TaqI TCGA 7 cut(s) 319, 366, 462, 640, 899, 1535, 1601
TasI AATT 7 cut(s) 161, 362, 621, 658, 997, 1154, 1632
TatI WGTACW 4 cut(s) 865, 889, 1062, 1078
TauI GCSGC 4 cut(s) 141, 188, 448, 1099
TfiI GAWTC 5 cut(s) 126, 536, 680, 1598, 1668
Tru1I TTAA 1 cut(s) 827
Tru9I TTAA 1 cut(s) 827
TscAI CASTG 5 cut(s) 124, 619, 1108, 1615, 1701
TseFI GTSAC 1 cut(s) 1026
TseI GCWGC 6 cut(s) 174, 1162, 1165, 1402, 1527, 1580
Tsp45I GTSAC 1 cut(s) 1026
TspDTI ATGAA 6 cut(s) 280, 290, 1214, 1407, 1493, 1772
TspGWI ACGGA 3 cut(s) 169, 249, 299
TspRI CASTG 5 cut(s) 124, 619, 1108, 1615, 1701
Tth111I GACNNNGTC 1 cut(s) 1682
VpaK11BI GGWCC 3 cut(s) 310, 938, 1469
XapI RAATTY 3 cut(s) 621, 658, 997
XceI RCATGY 2 cut(s) 518, 1568
XhoI CTCGAG 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 1346
XspI CTAG 3 cut(s) 728, 1490, 1584
ZraI GACGTC 1 cut(s) 1732
ZrmI AGTACT 1 cut(s) 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.