pycom14g15170

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007090
Physical Location & Seq
Forward (+)
17875155 .. 17876709
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
ATGACATCGAAAGTTCAGTTTCCAAAGTCTCTCCTCCTTATTTCCAAAGTCTCTCCCTTAATTTGCTCTCTATTTCTTGCTTTTCATGCTTCCTTGGATTTTGGGTGTTGTGGCTTTTGCTTCTTTTTAGAGAGAGAGAGAGAGAGAGAGAGGATGAAGAGGTGTAACAGTGAGTCTTCTTCCAAACCGGACAGGAAGACAGTGGAAAGAAACAGAAGAATTCACATGAAAAGTCTCTGCTTCAAGCTTGCTTCACTTGTTCCTCCTCAACACTTCAAAACCACCAACTCCAAGACCATGTCACAGCAAAATCAGCTTGATACTGCGGCATCCTATATAAAGAAACTGAGAGAGAGAATCGAGAATCTGAAAGAGAGGAAGGAGAGAGCAATGAGGTCACAATTAGGCAGTTCTAAGTCTGACATTGGCTATATTGACCCAGAAAAAACCGCGATGATGGGCTCAAGATTGCCAGTTATTGAATTAAGAGACTCGGGTTCAAGCATAGAAGTGATGCTGATCAGTGGGTTGAATAAGAACTTCATGTTTTATGAAGTCATCAGTGTTCTTGAGGAAGAAGGAGCTGAAGTTGTCAGTGCTAGCTTTACCACCATAGGCGATAAAGTTTTCCACTCGGTGCATGCTCAGGTCAAAATTTCTAGAGTTGGAGTAGATACTTCGACGGTATTGCAGAGACTACAGGATTTGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

238

Amino Acids

26.94

Weight (kDa)

9.42

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 67 - 117 4.2e-06 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 452
AciI CCGC 2 cut(s) 326, 450
AcsI RAATTY 2 cut(s) 219, 654
AcuI CTGAAG 1 cut(s) 606
AdeI CACNNNGTG 1 cut(s) 637
AgsI TTSAA 5 cut(s) 244, 277, 482, 501, 532
AluBI AGCT 4 cut(s) 247, 316, 584, 603
AluI AGCT 4 cut(s) 247, 316, 584, 603
Alw26I GTCTC 5 cut(s) 33, 55, 239, 483, 688
Ama87I CYCGRG 1 cut(s) 493
ApoI RAATTY 2 cut(s) 219, 654
AsuNHI GCTAGC 1 cut(s) 599
AvaI CYCGRG 1 cut(s) 493
BanII GRGCYC 1 cut(s) 464
BbsI GAAGAC 2 cut(s) 168, 203
BccI CCATC 1 cut(s) 451
BcgI CGANNNNNNTGC 2 cut(s) 670, 704
BclI TGATCA 1 cut(s) 519
BcoDI GTCTC 5 cut(s) 33, 55, 239, 483, 688
BfaI CTAG 2 cut(s) 600, 660
BfmI CTRYAG 1 cut(s) 698
BisI GCNGC 1 cut(s) 327
BlsI GCNGC 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 493
BmsI GCATC 2 cut(s) 338, 504
BmtI GCTAGC 1 cut(s) 603
BpiI GAAGAC 2 cut(s) 168, 203
Bpu10I CCTNAGC 1 cut(s) 645
BpuEI CTTGAG 2 cut(s) 448, 590
BsaBI GATNNNNATC 1 cut(s) 518
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bse1I ACTGG 1 cut(s) 473
Bse3DI GCAATG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 518
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 159, 329
BseJI GATNNNNATC 1 cut(s) 518
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 2 cut(s) 338, 659
BseNI ACTGG 1 cut(s) 473
BseRI GAGGAG 2 cut(s) 23, 255
Bsh1236I CGCG 1 cut(s) 452
BsiHKCI CYCGRG 1 cut(s) 493
BsiSI CCGG 1 cut(s) 188
BsmAI GTCTC 5 cut(s) 33, 55, 239, 483, 688
BsoBI CYCGRG 1 cut(s) 493
Bsp1286I GDGCHC 1 cut(s) 464
Bsp143I GATC 1 cut(s) 519
BspACI CCGC 2 cut(s) 326, 450
BspCNI CTCAG 2 cut(s) 339, 658
BspFNI CGCG 1 cut(s) 452
BspOI GCTAGC 1 cut(s) 603
BsrDI GCAATG 1 cut(s) 396
BsrI ACTGG 1 cut(s) 473
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 93
Bst4CI ACNGT 3 cut(s) 170, 202, 685
Bst6I CTCTTC 1 cut(s) 152
BstC8I GCNNGC 3 cut(s) 249, 601, 642
BstDEI CTNAG 3 cut(s) 347, 414, 645
BstF5I GGATG 2 cut(s) 159, 329
BstFNI CGCG 1 cut(s) 452
BstKTI GATC 1 cut(s) 522
BstMAI GTCTC 5 cut(s) 33, 55, 239, 483, 688
BstMBI GATC 1 cut(s) 519
BstMWI GCNNNNNNNGC 2 cut(s) 86, 313
BstNSI RCATGY 1 cut(s) 644
BstSFI CTRYAG 1 cut(s) 698
BstUI CGCG 1 cut(s) 452
BstV2I GAAGAC 2 cut(s) 168, 203
BtgZI GCGATG 1 cut(s) 467
BtsCI GGATG 2 cut(s) 159, 329
BtsIMutI CAGTG 5 cut(s) 175, 207, 529, 568, 601
Cac8I GCNNGC 3 cut(s) 249, 601, 642
CviAII CATG 5 cut(s) 86, 226, 298, 544, 641
CviJI RGCY 7 cut(s) 114, 247, 316, 429, 462, 584, 603
CviKI_1 RGCY 7 cut(s) 114, 247, 316, 429, 462, 584, 603
DdeI CTNAG 3 cut(s) 347, 414, 645
DpnI GATC 1 cut(s) 521
DpnII GATC 1 cut(s) 519
DraIII CACNNNGTG 1 cut(s) 637
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 464
Eco57I CTGAAG 1 cut(s) 606
Eco88I CYCGRG 1 cut(s) 493
EcoRI GAATTC 1 cut(s) 219
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 464
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 5 cut(s) 89, 229, 301, 547, 644
FatI CATG 5 cut(s) 85, 225, 297, 543, 640
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 1 cut(s) 327
FokI GGATG 2 cut(s) 166, 316
FriOI GRGCYC 1 cut(s) 464
Fsp4HI GCNGC 1 cut(s) 327
FspBI CTAG 2 cut(s) 600, 660
GluI GCNGC 1 cut(s) 327
HapII CCGG 1 cut(s) 188
Hin1II CATG 5 cut(s) 89, 229, 301, 547, 644
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 4 cut(s) 173, 357, 364, 491
HpaII CCGG 1 cut(s) 188
Hpy188I TCNGA 2 cut(s) 369, 421
Hpy188III TCNNGA 4 cut(s) 361, 465, 569, 660
Hpy99I CGWCG 1 cut(s) 685
HpyAV CCTTC 2 cut(s) 373, 572
HpyCH4III ACNGT 3 cut(s) 170, 202, 685
HpyCH4V TGCA 2 cut(s) 640, 691
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 313
HpyF3I CTNAG 3 cut(s) 347, 414, 645
Hsp92II CATG 5 cut(s) 89, 229, 301, 547, 644
Ksp22I TGATCA 1 cut(s) 519
Kzo9I GATC 1 cut(s) 519
LmnI GCTCC 1 cut(s) 581
LpnPI CCDG 6 cut(s) 178, 201, 453, 486, 632, 686
LweI GCATC 2 cut(s) 338, 504
MaeI CTAG 2 cut(s) 600, 660
MaeIII GTNAC 3 cut(s) 164, 300, 396
MalI GATC 1 cut(s) 521
MboI GATC 1 cut(s) 519
MboII GAAGA 6 cut(s) 168, 169, 171, 208, 228, 587
MhlI GDGCHC 1 cut(s) 464
MluCI AATT 5 cut(s) 60, 219, 401, 482, 654
MlyI GAGTC 2 cut(s) 182, 485
MmeI TCCRAC 1 cut(s) 646
MnlI CCTC 8 cut(s) 44, 144, 153, 273, 276, 369, 387, 565
MseI TTAA 2 cut(s) 59, 485
MslI CAYNNNNRTG 1 cut(s) 509
MspI CCGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 2 cut(s) 86, 313
NdeII GATC 1 cut(s) 519
NheI GCTAGC 1 cut(s) 599
NlaIII CATG 5 cut(s) 89, 229, 301, 547, 644
NmuCI GTSAC 2 cut(s) 300, 396
NspI RCATGY 1 cut(s) 644
PaeI GCATGC 1 cut(s) 644
PfeI GAWTC 2 cut(s) 357, 364
PflFI GACNNNGTC 1 cut(s) 298
PkrI GCNGC 1 cut(s) 328
PleI GAGTC 2 cut(s) 181, 485
PpsI GAGTC 2 cut(s) 181, 485
PsyI GACNNNGTC 1 cut(s) 298
RseI CAYNNNNRTG 1 cut(s) 509
SaqAI TTAA 2 cut(s) 59, 485
SatI GCNGC 1 cut(s) 327
Sau3AI GATC 1 cut(s) 519
SchI GAGTC 2 cut(s) 182, 485
SduI GDGCHC 1 cut(s) 464
SetI ASST 7 cut(s) 164, 249, 318, 398, 586, 605, 651
SfaNI GCATC 2 cut(s) 338, 504
SfcI CTRYAG 1 cut(s) 698
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 2 cut(s) 463, 569
SmoI CTYRAG 2 cut(s) 463, 569
SphI GCATGC 1 cut(s) 644
Sse9I AATT 5 cut(s) 60, 219, 401, 482, 654
SsiI CCGC 2 cut(s) 326, 450
SspMI CTAG 2 cut(s) 600, 660
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 3 cut(s) 170, 202, 685
TaqI TCGA 3 cut(s) 8, 360, 680
TasI AATT 5 cut(s) 60, 219, 401, 482, 654
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 2 cut(s) 357, 364
Tru1I TTAA 2 cut(s) 59, 485
Tru9I TTAA 2 cut(s) 59, 485
TscAI CASTG 5 cut(s) 175, 207, 529, 568, 601
TseFI GTSAC 2 cut(s) 300, 396
Tsp45I GTSAC 2 cut(s) 300, 396
TspDTI ATGAA 5 cut(s) 74, 170, 242, 532, 567
TspRI CASTG 5 cut(s) 175, 207, 529, 568, 601
Tth111I GACNNNGTC 1 cut(s) 298
XapI RAATTY 2 cut(s) 219, 654
XbaI TCTAGA 1 cut(s) 659
XceI RCATGY 1 cut(s) 644
XspI CTAG 2 cut(s) 600, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.