pycom14g15690

Pentatricopeptide repeat-containing protein At1g74850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009497
Physical Location & Seq
Forward (+)
18233253 .. 18237403
4151 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2748 bp
ATGCGTGGTGCGACAGTTGGGTTGGGTGGCGGCGATTGCGGGCCTGCTTACTTACTTACTGTGGGCGCGGTTCGGAGAAAATCCAATCCGAAGCTCCGAGAGGCTCATGCGAGCCTACTTACCGAAATGTCCTTCTCCCCCACGCTCTCCATTTCCAGCTCTTCGCCTCTCTCCACCTCAATTTCCAAAATTAACCCCAAGTCCCACCACCTCACCGTTCTCACCAACTCATCAACCCACCGGAGATTCCTCTCCGGCCGCACGACCTTCCTCTCCGGCGACCGCCGGCTGGAGTTCTTGTCCAGGGCGAAGCCGAAGGACCTCATCCTCGGGAACCCGTCGGTGACGGTGGAGAAGGGGAAGTACAGCTACGACGTCGAAACCCTAATCAACAAGCTCAGCAGCCTCCCTCCGCGCGGCAGCATCGCTCGGTGCCTCGACATCTTCAAGAACAAGCTGTCACTAAACGACTTCGCTTTGGTGTTCAAGGAGTTCGCGGCGCGCGGCGATTGGCAGCGGTCGCTTCGCCTCTTCAAGTATATGCAGCGCCAGATATGGTGCAAGCCGAACGAGCACATATACACGATAATGATCAGCTTGCTCGGCCGTGAGGGGCTGCTGGATAAGTGCGCCGAGGTGTTCGACGAAATGCCTAGCCAGGGCGTGGTCCGCAGCGTCTTCAGCTACACCGCATTGATCAATGCCTACGGGCGTAACGGTCAGTATGAAACATCCCTTGAATTTCTTGATCGAATGAAGAAAGATAAGGTTCCGCCGAGTATTTTGACTTACAATACTGTGCTCAATGCCTGTGCTAGAGGTGGGCTGGATTGGGAAGGGTTATTAGGATTGTTTGCCGAAATGCGGCACGAAGGAATACAGCCTGACCTTGTTACTTATAATACTTTGCTTAGTGCTTGTGCTGGTAGAGGGTTAGGCGATGAGGCAGAGATGGTGTTCAGGACTATGAATGAGGGTGGGATTGTTCCAGATATGACCACGTATCGTTACCTTGTTGAAACTTTTGGTAAATTGGACAAGCTCGAGAAAGTCTCGGAGCTTCTTAAAGAGATGGAAGCTGGAGGGAATTTGCCTGATATTACGTCGTATAATGTGTTACTGGAGGCATATGCTGAATTGGGATCGATTAGAGAGTCAATGGGTGTGTTTAGGCAGATGCAGGCTGCGGGGTGTATGCCGAATGCTGCTACTTATAGTATTTTGCTGAATTTGTATGGGAGGCATGGAAGGTATGATGATGTTCGAGAGCTTTTTCTTGAGATGAAAGTGAGCAATACGGAGCCAGATGCAGCTACATATAACATTCTTATACAGGTGTTTGGGGAGGGTGGGTATTTTAAGGAAGTAGTGACTTTGTTTCATGATATGGTGGAGGAGAAAATCGAGCCGAATATGGAGACGTATGAAGGGTTGATATATGCTTGTGGAAAGGGAGGACTCCATGAGGATTCCAAGAAAATTTTACATCACATGCACGAGAATGGAATAGTGCCTAGTACCAAGGCTTATACAGGGGTTATTGAAGCATATGGGCAAGCAGCATTGTATGACGAAGCTCTTGTTGCCTTCAACACAATGAATGAAGTGGGAAGCAAACCATCCGTTGAAAGCTACAACTCACTGATATATGCATTTGCAAGAGGCGGACTATACAGGGAGACTGAAGCAGTCTTGTTAATAATGGGCAAGGCTGGTGTTGCCCGGAATGTCCATACAGTCAATGGCATGATAGAAGCTTTTAGGCAAGGAGGTCAATTTGAAGAAGCTATCAAGGCATACGTTGAGATGGAAAAGACAAGATGCGATCCCGATGAGTGGACCCTTGAGGCAGTTTTAAGCGTTTACTGCGTTGCAGGTCTTGTTAATGAGTGTGAGGAGCACTTCCAAGAGATTAAAGCCTCAGGAATATTACCCAGTGTCATGTGCTACTGCATGATGCTTGCTGTTTATGCAAGGAGTGACAGGTGGCATGATGCCAATGTGTTGCTGAATGAGATGCTTACAAACAGGGCATCGAATATTCATCAAGTGATTGGGCAGATGATCAAGGGAGATTATGATGATGACTCTAACTGGCAGATGGTAGAGTATGTTTTTGACAAATTAAAATCCGAAGGATGCGGCTTGGGAATGAGGTTCTACAACACGCTACTAGAAGCACTTTGGTGGTTGGGTCAGAAACAAAGAGCTGCGCGAGTTCTTAATGAAGCAACACAGCGGGGGCTTTTTCCTGAACTTTTTCGTAAAAATAAACTTGTGGTGTCTGTTGATGTGCACAGAATGTGGCAAGGCGGTGCATATACAGCAATGTCAGTTTGGCTAAACAATATGTATGAGATGTTCCTCAATGGCGAGGATCTACCCCATCTTGCAACTGTTGTTGTAGTACGAGGGAAGATGGAGAAAAGCTCAATAACACAAGATCTACCGATCGCAAAGGCTGCTTATACATTTCTGCAGGATAATATGCCAGCTTCCTTTTCTTTCCCAAAATGGAACAATGGTCGAATTCTCTGTCAGCGCCCTCAGCTCAAGCGGATCCTATCAAGCATCGAACCATCCACAGATGAGTCCGAAAGGAATAATATTATCACGTTAAGTAACTCCCCGTTTCCTCCTCCTGGAACAAAGACATCCTCCAGTGCTGTCAATAGCGGACGGTATAATGATGCCGGTTCTGATGAAAGAATTAGGACAAGAACGGAGGTTTTGACAAGCATGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

916

Amino Acids

102.48

Weight (kDa)

6.13

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 172 - 275 8.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 189 - 234 6e-08 PPR repeat family
PPR PF01535 193 - 222 4.4e-06 PPR repeat
TPR_24 PF23276 202 - 308 8.6e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 213 - 271 5.7e-14 Pentatricopeptide repeat domain
PPR_2 PF13041 225 - 273 7.3e-13 PPR repeat family
PPR PF01535 227 - 256 5.8e-07 PPR repeat
PPR_long PF17177 240 - 382 3.8e-11 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 266 - 393 1.2e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 282 - 308 4.3e-06 PPR repeat family
PPR_3 PF13812 284 - 344 6.3e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 292 - 323 7e-06 PPR repeat
PPR_2 PF13041 295 - 342 1.5e-10 PPR repeat family
PPR_3 PF13812 354 - 413 8.3e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 365 - 410 3.5e-11 PPR repeat family
PPR PF01535 404 - 431 6.5e-06 PPR repeat
PPR_2 PF13041 407 - 447 2.6e-09 PPR repeat family
PPR_3 PF13812 424 - 484 1.5e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 439 - 484 6.2e-09 PPR repeat family
PPR_3 PF13812 459 - 519 5e-07 Pentatricopeptide repeat domain
TPR_24 PF23276 491 - 602 1.3e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 493 - 552 5.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 505 - 554 3.2e-06 PPR repeat family
PPR_2 PF13041 575 - 623 3.2e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 900
AatII GACGTC 1 cut(s) 378
Acc36I ACCTGC 1 cut(s) 1865
AccB1I GGYRCC 1 cut(s) 432
AccB7I CCANNNNNTGG 1 cut(s) 664
AccII CGCG 7 cut(s) 68, 415, 417, 497, 502, 504, 2215
AclWI GGATC 5 cut(s) 1150, 1820, 2385, 2554, 2567
AcoI YGGCCR 2 cut(s) 256, 604
AcsI RAATTY 5 cut(s) 740, 1087, 1228, 1479, 2529
AcuI CTGAAG 2 cut(s) 664, 1704
AcyI GRCGYC 1 cut(s) 375
AfaI GTAC 3 cut(s) 365, 1519, 2409
AfiI CCNNNNNNNGG 7 cut(s) 289, 416, 659, 664, 864, 1836, 2642
AgsI TTSAA 9 cut(s) 448, 487, 535, 740, 1019, 1544, 1591, 1628, 1781
AjnI CCWGG 3 cut(s) 302, 657, 2641
AleI CACNNNNGTG 1 cut(s) 2185
Alw21I GWGCWC 4 cut(s) 576, 804, 1902, 2298
Alw26I GTCTC 3 cut(s) 1057, 1412, 1673
Alw44I GTGCAC 1 cut(s) 2294
AlwI GGATC 5 cut(s) 1150, 1820, 2385, 2554, 2567
Ama87I CYCGRG 2 cut(s) 329, 1043
AoxI GGCC 3 cut(s) 41, 256, 604
ApaLI GTGCAC 1 cut(s) 2294
ApoI RAATTY 5 cut(s) 740, 1087, 1228, 1479, 2529
ArsI GACNNNNNNTTYG 2 cut(s) 2521, 2553
Asp700I GAANNNNTTC 1 cut(s) 2259
AspLEI GCGC 8 cut(s) 68, 417, 502, 504, 549, 632, 2215, 2544
AspS9I GGNCC 4 cut(s) 41, 319, 667, 1839
AsuC2I CCSGG 1 cut(s) 1723
AsuHPI GGTGA 3 cut(s) 205, 214, 355
AvaI CYCGRG 2 cut(s) 329, 1043
AvaII GGWCC 3 cut(s) 319, 667, 1839
AxyI CCTNAGG 1 cut(s) 1921
BaeGI GKGCMC 1 cut(s) 2298
BamHI GGATCC 1 cut(s) 2559
BanI GGYRCC 1 cut(s) 432
BarI GAAGNNNNNNTAC 4 cut(s) 347, 353, 379, 385
BauI CACGAG 1 cut(s) 1496
BbsI GAAGAC 1 cut(s) 670
Bbv12I GWGCWC 4 cut(s) 576, 804, 1902, 2298
BbvCI CCTCAGC 1 cut(s) 2547
BccI CCATC 8 cut(s) 946, 1066, 1627, 1801, 2095, 2394, 2413, 2587
BceAI ACGGC 1 cut(s) 591
BciT130I CCWGG 3 cut(s) 304, 659, 2643
BclI TGATCA 3 cut(s) 591, 696, 2064
BcnI CCSGG 1 cut(s) 1723
BcoDI GTCTC 3 cut(s) 1057, 1412, 1673
BfaI CTAG 4 cut(s) 654, 816, 1515, 2174
BfmI CTRYAG 1 cut(s) 2477
BfoI RGCGCY 2 cut(s) 550, 2545
BfuAI ACCTGC 1 cut(s) 1865
BglII AGATCT 1 cut(s) 2443
BlpI GCTNAGC 1 cut(s) 398
Bme1390I CCNGG 4 cut(s) 304, 659, 1723, 2643
Bme18I GGWCC 3 cut(s) 319, 667, 1839
BmeT110I CYCGRG 2 cut(s) 329, 1043
BmgT120I GGNCC 4 cut(s) 41, 319, 667, 1839
BmiI GGNNCC 6 cut(s) 335, 434, 771, 1302, 1841, 2561
BmrFI CCNGG 4 cut(s) 304, 659, 1723, 2643
BmrI ACTGGG 1 cut(s) 1929
BmuI ACTGGG 1 cut(s) 1929
BpiI GAAGAC 1 cut(s) 670
BplI GAGNNNNNCTC 4 cut(s) 1037, 1069, 2414, 2446
BpmI CTGGAG 4 cut(s) 311, 1101, 1142, 2644
Bpu10I CCTNAGC 1 cut(s) 2547
Bpu1102I GCTNAGC 1 cut(s) 398
BpuEI CTTGAG 3 cut(s) 1298, 1865, 2537
BpuMI CCSGG 1 cut(s) 1723
Bsa29I ATCGAT 1 cut(s) 1145
BsaAI YACGTR 1 cut(s) 1002
BsaBI GATNNNNATC 1 cut(s) 590
BsaHI GRCGYC 1 cut(s) 375
BsaJI CCNNGG 5 cut(s) 303, 328, 633, 658, 1521
BsaWI WCCGGW 1 cut(s) 240
BsaXI ACNNNNNCTCC 4 cut(s) 284, 314, 1337, 1367
Bsc4I CCNNNNNNNGG 7 cut(s) 289, 416, 659, 664, 864, 1836, 2642
Bse118I RCCGGY 2 cut(s) 285, 2693
Bse1I ACTGG 4 cut(s) 1125, 1935, 2099, 2661
Bse21I CCTNAGG 1 cut(s) 1921
Bse3DI GCAATG 1 cut(s) 2334
Bse8I GATNNNNATC 1 cut(s) 590
BseBI CCWGG 3 cut(s) 304, 659, 2643
BseCI ATCGAT 1 cut(s) 1145
BseDI CCNNGG 5 cut(s) 303, 328, 633, 658, 1521
BseGI GGATG 6 cut(s) 324, 731, 1619, 2144, 2579, 2654
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 7 cut(s) 289, 416, 659, 664, 864, 1836, 2642
BseMI GCAATG 1 cut(s) 2334
BseMII CTCAG 3 cut(s) 412, 1935, 2561
BseNI ACTGG 4 cut(s) 1125, 1935, 2099, 2661
BsePI GCGCGC 1 cut(s) 500
BseRI GAGGAG 3 cut(s) 1409, 1910, 2628
BseSI GKGCMC 1 cut(s) 2298
BseX3I CGGCCG 2 cut(s) 256, 604
Bsh1236I CGCG 7 cut(s) 68, 415, 417, 497, 502, 504, 2215
Bsh1285I CGRYCG 5 cut(s) 259, 283, 521, 607, 2454
BshFI GGCC 3 cut(s) 43, 258, 606
BshNI GGYRCC 1 cut(s) 432
BshVI ATCGAT 1 cut(s) 1145
BsiEI CGRYCG 5 cut(s) 259, 283, 521, 607, 2454
BsiHKAI GWGCWC 4 cut(s) 576, 804, 1902, 2298
BsiHKCI CYCGRG 2 cut(s) 329, 1043
BsiSI CCGG 6 cut(s) 241, 255, 276, 286, 1723, 2694
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 7 cut(s) 289, 416, 659, 664, 864, 1836, 2642
BsmAI GTCTC 3 cut(s) 1057, 1412, 1673
BsmBI CGTCTC 1 cut(s) 1412
BsmFI GGGAC 1 cut(s) 187
BsmI GAATGC 1 cut(s) 1207
BsnI GGCC 3 cut(s) 43, 258, 606
BsoBI CYCGRG 2 cut(s) 329, 1043
Bsp1286I GDGCHC 4 cut(s) 576, 804, 1902, 2298
Bsp1720I GCTNAGC 1 cut(s) 398
BspANI GGCC 3 cut(s) 43, 258, 606
BspCNI CTCAG 3 cut(s) 411, 1934, 2560
BspDI ATCGAT 1 cut(s) 1145
BspFNI CGCG 7 cut(s) 68, 415, 417, 497, 502, 504, 2215
BspHI TCATGA 1 cut(s) 1381
BspLI GGNNCC 6 cut(s) 335, 434, 771, 1302, 1841, 2561
BspMAI CTGCAG 1 cut(s) 2481
BspMI ACCTGC 1 cut(s) 1865
BspPI GGATC 5 cut(s) 1150, 1820, 2385, 2554, 2567
BspQI GCTCTTC 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 2334
BsrFI RCCGGY 2 cut(s) 285, 2693
BsrI ACTGG 4 cut(s) 1125, 1935, 2099, 2661
BssAI RCCGGY 2 cut(s) 285, 2693
BssECI CCNNGG 5 cut(s) 303, 328, 633, 658, 1521
BssHII GCGCGC 1 cut(s) 500
BssNI GRCGYC 1 cut(s) 375
BssSI CACGAG 1 cut(s) 1496
BssT1I CCWWGG 1 cut(s) 1521
Bst2BI CACGAG 1 cut(s) 1496
Bst2UI CCWGG 3 cut(s) 304, 659, 2643
Bst4CI ACNGT 9 cut(s) 16, 61, 217, 349, 719, 799, 1738, 2398, 2682
Bst6I CTCTTC 2 cut(s) 166, 536
BstACI GRCGYC 1 cut(s) 375
BstAPI GCANNNNNTGC 1 cut(s) 2462
BstBAI YACGTR 1 cut(s) 1002
BstDEI CTNAG 4 cut(s) 398, 911, 1921, 2547
BstF5I GGATG 6 cut(s) 324, 731, 1619, 2144, 2579, 2654
BstFNI CGCG 7 cut(s) 68, 415, 417, 497, 502, 504, 2215
BstH2I RGCGCY 2 cut(s) 550, 2545
BstHHI GCGC 8 cut(s) 68, 417, 502, 504, 549, 632, 2215, 2544
BstMAI GTCTC 3 cut(s) 1057, 1412, 1673
BstMCI CGRYCG 5 cut(s) 259, 283, 521, 607, 2454
BstNI CCWGG 3 cut(s) 304, 659, 2643
BstNSI RCATGY 1 cut(s) 1495
BstSCI CCNGG 4 cut(s) 302, 657, 1721, 2641
BstSFI CTRYAG 1 cut(s) 2477
BstSLI GKGCMC 1 cut(s) 2298
BstUI CGCG 7 cut(s) 68, 415, 417, 497, 502, 504, 2215
BstV2I GAAGAC 1 cut(s) 670
BstX2I RGATCY 3 cut(s) 2377, 2443, 2559
BstYI RGATCY 3 cut(s) 2377, 2443, 2559
BstZI CGGCCG 2 cut(s) 256, 604
Bsu15I ATCGAT 1 cut(s) 1145
Bsu36I CCTNAGG 1 cut(s) 1921
BsuRI GGCC 3 cut(s) 43, 258, 606
BsuTUI ATCGAT 1 cut(s) 1145
BtgZI GCGATG 2 cut(s) 409, 954
BtsCI GGATG 6 cut(s) 324, 731, 1619, 2144, 2579, 2654
BtsIMutI CAGTG 3 cut(s) 1640, 1942, 2668
BveI ACCTGC 1 cut(s) 1865
CciI TCATGA 1 cut(s) 1381
CfoI GCGC 8 cut(s) 68, 417, 502, 504, 549, 632, 2215, 2544
Cfr10I RCCGGY 2 cut(s) 285, 2693
Cfr13I GGNCC 4 cut(s) 41, 319, 667, 1839
ClaI ATCGAT 1 cut(s) 1145
CseI GACGC 1 cut(s) 664
Csp6I GTAC 3 cut(s) 364, 1518, 2408
CviQI GTAC 3 cut(s) 364, 1518, 2408
DdeI CTNAG 4 cut(s) 398, 911, 1921, 2547
EaeI YGGCCR 2 cut(s) 256, 604
EagI CGGCCG 2 cut(s) 256, 604
Eam1104I CTCTTC 2 cut(s) 166, 536
EarI CTCTTC 2 cut(s) 166, 536
EciI GGCGGA 2 cut(s) 762, 1680
EclXI CGGCCG 2 cut(s) 256, 604
Eco130I CCWWGG 1 cut(s) 1521
Eco47I GGWCC 3 cut(s) 319, 667, 1839
Eco52I CGGCCG 2 cut(s) 256, 604
Eco57I CTGAAG 2 cut(s) 664, 1704
Eco81I CCTNAGG 1 cut(s) 1921
Eco88I CYCGRG 2 cut(s) 329, 1043
EcoO109I RGGNCCY 1 cut(s) 319
EcoRI GAATTC 1 cut(s) 2529
EcoRII CCWGG 3 cut(s) 302, 657, 2641
EcoT14I CCWWGG 1 cut(s) 1521
EcoT22I ATGCAT 1 cut(s) 1654
ErhI CCWWGG 1 cut(s) 1521
Esp3I CGTCTC 1 cut(s) 1412
FaqI GGGAC 1 cut(s) 187
FauI CCCGC 3 cut(s) 32, 1180, 2232
FauNDI CATATG 2 cut(s) 1129, 1549
FbaI TGATCA 3 cut(s) 591, 696, 2064
FokI GGATG 6 cut(s) 311, 718, 1606, 2151, 2566, 2641
FspBI CTAG 4 cut(s) 654, 816, 1515, 2174
GlaI GCGC 8 cut(s) 67, 416, 501, 503, 548, 631, 2214, 2543
GsuI CTGGAG 4 cut(s) 311, 1101, 1142, 2644
HaeII RGCGCY 2 cut(s) 550, 2545
HaeIII GGCC 3 cut(s) 43, 258, 606
HapII CCGG 6 cut(s) 241, 255, 276, 286, 1723, 2694
HgaI GACGC 1 cut(s) 664
HhaI GCGC 8 cut(s) 68, 417, 502, 504, 549, 632, 2215, 2544
Hin1I GRCGYC 1 cut(s) 375
Hin6I GCGC 8 cut(s) 66, 415, 500, 502, 547, 630, 2213, 2542
HinP1I GCGC 8 cut(s) 66, 415, 500, 502, 547, 630, 2213, 2542
HindIII AAGCTT 1 cut(s) 1755
HinfI GANTC 6 cut(s) 246, 1154, 1458, 1469, 2087, 2591
HpaII CCGG 6 cut(s) 241, 255, 276, 286, 1723, 2694
HphI GGTGA 3 cut(s) 205, 214, 355
Hpy166II GTNNAC 3 cut(s) 1839, 1864, 2296
Hpy188I TCNGA 8 cut(s) 75, 90, 98, 1057, 2134, 2199, 2596, 2701
Hpy8I GTNNAC 3 cut(s) 1839, 1864, 2296
Hpy99I CGWCG 5 cut(s) 343, 377, 380, 647, 1108
HpyCH4III ACNGT 9 cut(s) 16, 61, 217, 349, 719, 799, 1738, 2398, 2682
HpyCH4IV ACGT 6 cut(s) 375, 1001, 1103, 1421, 1800, 2615
HpyF3I CTNAG 4 cut(s) 398, 911, 1921, 2547
HpySE526I ACGT 6 cut(s) 375, 1001, 1103, 1421, 1800, 2615
Hsp92I GRCGYC 1 cut(s) 375
HspAI GCGC 8 cut(s) 66, 415, 500, 502, 547, 630, 2213, 2542
KroI GCCGGC 1 cut(s) 285
KroNI GCCGGC 1 cut(s) 287
Ksp22I TGATCA 3 cut(s) 591, 696, 2064
LguI GCTCTTC 1 cut(s) 166
LmnI GCTCC 4 cut(s) 99, 1057, 1300, 1897
MaeI CTAG 4 cut(s) 654, 816, 1515, 2174
MaeII ACGT 6 cut(s) 375, 1001, 1103, 1421, 1800, 2615
MaeIII GTNAC 9 cut(s) 343, 459, 713, 892, 1007, 1116, 1369, 1979, 2621
MboII GAAGA 7 cut(s) 153, 436, 523, 670, 769, 1793, 2428
MflI RGATCY 3 cut(s) 2377, 2443, 2559
MhlI GDGCHC 4 cut(s) 576, 804, 1902, 2298
MlyI GAGTC 4 cut(s) 1163, 1452, 2081, 2600
Mph1103I ATGCAT 1 cut(s) 1654
MroNI GCCGGC 1 cut(s) 285
MroXI GAANNNNTTC 1 cut(s) 2259
MslI CAYNNNNRTG 3 cut(s) 587, 1500, 2185
MspA1I CMGCKG 2 cut(s) 517, 2239
MspI CCGG 6 cut(s) 241, 255, 276, 286, 1723, 2694
MspR9I CCNGG 4 cut(s) 304, 659, 1723, 2643
Mva1269I GAATGC 1 cut(s) 1207
MvaI CCWGG 3 cut(s) 304, 659, 2643
MvnI CGCG 7 cut(s) 68, 415, 417, 497, 502, 504, 2215
NaeI GCCGGC 1 cut(s) 287
NciI CCSGG 1 cut(s) 1723
NdeI CATATG 2 cut(s) 1129, 1549
NgoMIV GCCGGC 1 cut(s) 285
NlaIV GGNNCC 6 cut(s) 335, 434, 771, 1302, 1841, 2561
NmeAIII GCCGAG 3 cut(s) 582, 658, 801
NmuCI GTSAC 4 cut(s) 343, 459, 1369, 1979
NsiI ATGCAT 1 cut(s) 1654
NspI RCATGY 1 cut(s) 1495
OliI CACNNNNGTG 1 cut(s) 2185
PaeR7I CTCGAG 1 cut(s) 1043
PagI TCATGA 1 cut(s) 1381
PauI GCGCGC 1 cut(s) 500
PciSI GCTCTTC 1 cut(s) 166
PctI GAATGC 1 cut(s) 1207
PdiI GCCGGC 1 cut(s) 287
PdmI GAANNNNTTC 1 cut(s) 2259
PfeI GAWTC 2 cut(s) 246, 1469
PflMI CCANNNNNTGG 1 cut(s) 664
PfoI TCCNGGA 1 cut(s) 2641
Ple19I CGATCG 1 cut(s) 2454
PleI GAGTC 4 cut(s) 1162, 1452, 2081, 2599
PpsI GAGTC 4 cut(s) 1162, 1452, 2081, 2599
Ppu21I YACGTR 1 cut(s) 1002
PpuMI RGGWCCY 1 cut(s) 319
PsiI TTATAA 1 cut(s) 900
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 3 cut(s) 302, 657, 2641
PspGI CCWGG 3 cut(s) 302, 657, 2641
PspN4I GGNNCC 6 cut(s) 335, 434, 771, 1302, 1841, 2561
PspPI GGNCC 4 cut(s) 41, 319, 667, 1839
PspPPI RGGWCCY 1 cut(s) 319
PsrI GAACNNNNNNTAC 2 cut(s) 2345, 2377
PstI CTGCAG 1 cut(s) 2481
PsuI RGATCY 3 cut(s) 2377, 2443, 2559
PteI GCGCGC 1 cut(s) 500
PvuI CGATCG 1 cut(s) 2454
RsaI GTAC 3 cut(s) 365, 1519, 2409
RsaNI GTAC 3 cut(s) 364, 1518, 2408
RseI CAYNNNNRTG 3 cut(s) 587, 1500, 2185
SapI GCTCTTC 1 cut(s) 166
Sau96I GGNCC 4 cut(s) 41, 319, 667, 1839
SchI GAGTC 4 cut(s) 1163, 1452, 2081, 2600
ScrFI CCNGG 4 cut(s) 304, 659, 1723, 2643
SduI GDGCHC 4 cut(s) 576, 804, 1902, 2298
SfcI CTRYAG 1 cut(s) 2477
Sfr274I CTCGAG 1 cut(s) 1043
SinI GGWCC 3 cut(s) 319, 667, 1839
SlaI CTCGAG 1 cut(s) 1043
SmiMI CAYNNNNRTG 3 cut(s) 587, 1500, 2185
SmlI CTYRAG 4 cut(s) 1043, 1277, 1844, 2552
SmoI CTYRAG 4 cut(s) 1043, 1277, 1844, 2552
SspI AATATT 3 cut(s) 1929, 2041, 2608
SspMI CTAG 4 cut(s) 654, 816, 1515, 2174
StyD4I CCNGG 4 cut(s) 302, 657, 1721, 2641
StyI CCWWGG 1 cut(s) 1521
TaaI ACNGT 9 cut(s) 16, 61, 217, 349, 719, 799, 1738, 2398, 2682
TaiI ACGT 6 cut(s) 378, 1004, 1106, 1424, 1803, 2618
TatI WGTACW 1 cut(s) 363
TauI GCSGC 7 cut(s) 33, 261, 420, 500, 507, 868, 2145
TfiI GAWTC 2 cut(s) 246, 1469
TscAI CASTG 3 cut(s) 1647, 1942, 2668
TseFI GTSAC 4 cut(s) 343, 459, 1369, 1979
Tsp45I GTSAC 4 cut(s) 343, 459, 1369, 1979
TspGWI ACGGA 3 cut(s) 1313, 1612, 2738
TspRI CASTG 3 cut(s) 1647, 1942, 2668
Van91I CCANNNNNTGG 1 cut(s) 664
VneI GTGCAC 1 cut(s) 2294
VpaK11BI GGWCC 3 cut(s) 319, 667, 1839
XapI RAATTY 5 cut(s) 740, 1087, 1228, 1479, 2529
XceI RCATGY 1 cut(s) 1495
XcmI CCANNNNNNNNNTGG 1 cut(s) 1739
XhoI CTCGAG 1 cut(s) 1043
XmnI GAANNNNTTC 1 cut(s) 2259
XspI CTAG 4 cut(s) 654, 816, 1515, 2174
ZraI GACGTC 1 cut(s) 376
Zsp2I ATGCAT 1 cut(s) 1654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.