pycom14g18000

WD repeat and HMG-box DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005318
Physical Location & Seq
Forward (+)
19807044 .. 19812248
5205 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3063 bp
ATGATCATTGATTTTCCTATATCTTGCCAATATAGATTGAAGTTTACATTTCACCTCCCATTTCCATATCAATCATTGATTTTTCAAATATTTCAACTGAAATATCGACAATTTCATAGATATTTTAAACAGTGGTCGCAACAAAAACATATACCAATATTTACTGGTGGGCCTGTCACCCGAACTTTGAAGCTCCGAGAGGCCCACAAGCCCACGAGCAGCGGCGGGGGGCCGTCGTACTGCTCGGTGCTGTGGGACCAGCAGGCCTACCACGTCGTGACGGCCTCCTCCTCCGACCCCACAATCGCCATCCACGACTCTCTTATTCTGTCCAGCCCTCCCAAGCTCCTCCGCCACCACCGCGACGGCGTCACGGCTCTCGCTCTCAGCCCCAACTCCACCTGCCTCGCCTCCGGCTCCGTCGACCACTCCGTCAAGCTCTACAAGTTTCCAGGTGGAGAATTTCAGACAAATATAACCCGATTCACGCTGCCAATACGTACCCTGGCGTTTAACAAATCCGGGACACTGTTGGCAGCTGCTGGTGATGATGAAGGCATTAAGCTTATTAACACAGTTGATGGTTCAATTTCAAGAGTTCTTAAAGGACACAAAGGGCCAGTGACAGGTTTAGGTTTCGATCCCCACAGCGAATATTTGGCCTCTATTGATTCAACTGGGGCTGTTATATACTGGGAACTTTCATCTGGAACCACCTTGCATACCCTTAAAGGGGTAGCTCCTAACTCGGGTTTCGATCATTCCATAATGAATGTACTTAGCTGGAGTCCTGATGGAGACAAGTTAGCCGTGCCAGGGTTGAGAAATGATGTGGTGGTTTATGATAGAGACACAGCTGAAAAGCTGTTTTCGTTGAGAGGTGATCACACACAGCCTATTTGTTCCTTGGCTTGGTCACCTAACGGAAAATACATGGCTACTTCTGGTTTGGATAAGCAGGTTCTCATCTGGGATGTTGATCGGAAGCAGGACATTGACAGGCAGAACTTTGATGAGAGGATATGTTGCTTGGCGTGGAAGCCCATTGGCAATGCACTGGCTGTTATTGATTGTATGGGAAAGTATGGTGTGTGGGATTCAGTGACTCCTTCATCAATGAAGTCGCCCACTGAAGATGTTCCCAATCAACAATCTAAGAACAGCAATGGGCTGCTTTTGTTTGACGAAGAGGAGGGTCAGGAGCTTAGCACTTCTGGTAGTTTAAGTGATGTTGGTGAAGATAGTCTTGGGGAATCCAAACCGCCTAGCAGGAAGAGGCCACACAAGCACTATGCATATGAGGATGATTTGGAAGAGGATGGTTTTGATGGCTTTAGGTTGATACCAAATGTTGAGTTGGAGTCCCGAAAAAAAGCGCGTCGTAAACACAGTGAAAGTGTTGATAATGAGAATGAGGGAGTAAGCAGCAAAGTGACATCGATTAGATCAAAAATGCAGGTGGCATTCCAGCCTGGTGCTACTCCTTTGCAGCCTGGAAAAAGGCGCTTTCTGTGCTATAACATGCTTGGAGCCATAACTACAATTGAACATGACGGATTCTCCCATATTGAGATAGATTTTCATGATACAAGTCGCGGTCCAAGAGTTCCATCAATGAATGATCATTTTGGTTTCACAATGGCTTCACTTAATGAGAATGGAAGCGTTTTTGCAAATCCCTGCAAGGGAGAAAAGCACATGAGTACTCTCATGTATCGCCCATTCAGTAGTTGGGCAAATAATAGTGAGTGGTCTATGCGATTGGAGGGGGAAGAAGTGAAGGTTGTGGCACTTGGCACATCTTGGGTGGCAGCAATTACGAGTCTTAACTTCCTTCGCATCTTCACTGAAAGTGGTTTGCAGAAACATGTTGTCTCCCTTGATGGACCTGTCGTGACCGCATCAGGCTTCAAGGATGAACTTGCGATTGTCACTCATGCTTCCAATTGTCTTCCTTCAAATGAACAGATGCTTGAATTTAGAGTACTCAACATAACTAATGGAAGCCAGCCTCTTAGAGGACGGTTGCCTTTGACTCCTGGTTCACATCTAACATGGTTCGGGTTTAGTGAAGAAGGGCAATTAAGCTCCTACGATTCCAAGGGCGTGCTAAGAGTTTTTACAAGGCAATATGGTGGCAGTTGGCTCCCACTCTTCAGTGCTAGCAAAGAGAAGAAGTCAGGCGAAAACTATTGGGTGGTTGGGCTGAATGCAAGCAAATTGTTTTGCATAGTTTGCAAAAGTCCTGACTTGTACCCGCAGGTGATGCCGAAACCAGTCCTCACTCCGCTAAATTTTTCGTTCCCTCTAGCATCCTCTGATCTAGGGGCAGAAGCCCTTGAAAATGAGTTTATACTCAACAACACGCACCTCGCACAGATTCAAAAGAAAATAGAAGAACTGGATGCTGTCGGTTTGGACACCACTTCACTTGACGATGAAGCTTTCAATACAGAAGCAGCTCAAGACAGATGCATCTTAAAGCTTATTGCGTCCTGCTGCAACGGAGATAAGCTTGTGAGAGCAACTGAACTCGTGAAGCTTCTGTCCCTCGAAAAATCAGTGAGGGGTGCAATCAAGCTTGTCACTGTTCTGAAGCTCCCTAACTTGGCTGAAAGGTTCAACAGCATTTTGGAGGAAAGGCTGCTCAACGAGAGTAAAAGAGCTGTGGAAACCACCTTCCCTACCTCAAACCACAACGCATCTTTCATCACGGATGACATTGCAGCCGCAAAGAAAACAACCTCTACCGAGCCAAGTAAGTTGTCAGGAAACAACATTCCACTATCAACGCCAAAATTGTCGGCTCCTTTGTTTACGAAGAAAGCCAAAACACAAGAACCTAAAGTTGGAACAGAGAAAACAGACGAAAAACTGGCGGAGGATGATGTTGGGAAGGTAAAAACGACAGAGGCGAAAAATGCTACACAGGTGAGCAACAATGCCGGAGAGACCAAGAAGGTAGGGGAAGTGTCTGAGGTACCAAATGATCGAGAAAAAGTGAAGAAGGCAGAAGCTCCTCGACGAGCATCTAATCCATTCATGAAGAAATTAATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mcl1_mid WD40

Protein Analysis

1021

Amino Acids

112.67

Weight (kDa)

7.14

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 81 - 195 5.8e-14 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 82 - 364 5.4e-71 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 83 - 197 3.7e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 91 - 244 7.5e-23 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 97 - 241 1.7e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 115 - 148 6.3e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 116 - 245 1.4e-18 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 119 - 270 6.3e-11 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML PF23409 119 - 330 1.5e-09 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 121 - 356 8.9e-31 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 121 - 245 3.2e-09 WDR3 second beta-propeller domain
WDR55 PF24796 121 - 244 4.1e-08 WDR55
Beta-prop_WDR19_1st PF23389 173 - 308 1.6e-09 WDR19 first beta-propeller
Beta-prop_WDR35_TULP_N PF24797 196 - 327 6.9e-06 WDR35/TULP4 N-terminal
Beta-prop_CAF1B_HIR1 PF24105 201 - 334 1.3e-07 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 206 - 350 5.5e-14 CDC20/Fizzy WD40 domain
Beta-prop_RIG_2nd PF23775 216 - 352 9e-06 RIG second beta-propeller
Beta-prop_IFT122_1st PF23381 256 - 370 1.8e-07 IFT122 first beta-propeller
Beta-prop_THOC3 PF25174 257 - 376 7.2e-16 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 257 - 333 6.1e-09 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 258 - 371 3.6e-11 WDR5 beta-propeller domain
ANAPC4_WD40 PF12894 260 - 347 2.4e-07 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 260 - 334 7.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 265 - 357 3.9e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_Aladin PF25460 283 - 366 1.9e-07 Aladin seven-bladed propeller
WD40 PF00400 295 - 325 8.5e-08 WD domain, G-beta repeat
Mcl1_mid PF12341 488 - 770 2.4e-95 Minichromosome loss protein, Mcl1, middle region
Ctf4_C PF20946 779 - 882 2.9e-12 DNA polymerase alpha-binding protein Ctf4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 410, 1447, 2251
AasI GACNNNNNNGTC 1 cut(s) 431
Acc36I ACCTGC 4 cut(s) 410, 949, 1447, 2251
Acc65I GGTACC 1 cut(s) 2980
AccB1I GGYRCC 1 cut(s) 2980
AccI GTMKAC 1 cut(s) 423
AccII CGCG 3 cut(s) 363, 1378, 1596
AclWI GGATC 1 cut(s) 635
AcsI RAATTY 3 cut(s) 461, 1976, 2293
AcuI CTGAAG 3 cut(s) 1152, 2140, 2615
AcyI GRCGYC 1 cut(s) 369
AdeI CACNNNGTG 1 cut(s) 277
AfaI GTAC 7 cut(s) 239, 502, 777, 1705, 1986, 2255, 2982
AflIII ACRYGT 1 cut(s) 1867
AjiI CACGTC 1 cut(s) 274
AjnI CCWGG 6 cut(s) 451, 504, 814, 1471, 1492, 2038
AjuI GAANNNNNNNTTGG 4 cut(s) 1230, 1262, 2832, 2864
Alw26I GTCTC 4 cut(s) 792, 843, 1879, 2945
AlwI GGATC 1 cut(s) 635
AlwNI CAGNNNCTG 1 cut(s) 542
Ama87I CYCGRG 1 cut(s) 748
AoxI GGCC 8 cut(s) 170, 201, 230, 264, 282, 617, 660, 1277
ApoI RAATTY 3 cut(s) 461, 1976, 2293
AseI ATTAAT 1 cut(s) 3053
Asp700I GAANNNNTTC 1 cut(s) 1137
Asp718I GGTACC 1 cut(s) 2980
AspLEI GCGC 2 cut(s) 1378, 1506
AspS9I GGNCC 7 cut(s) 170, 202, 230, 256, 617, 1598, 1886
AsuC2I CCSGG 1 cut(s) 523
AsuHPI GGTGA 8 cut(s) 44, 169, 557, 893, 909, 1247, 2275, 2944
AsuNHI GCTAGC 1 cut(s) 2162
AvaI CYCGRG 1 cut(s) 748
AvaII GGWCC 3 cut(s) 256, 1598, 1886
BanI GGYRCC 1 cut(s) 2980
BauI CACGAG 2 cut(s) 214, 2534
BbsI GAAGAC 1 cut(s) 1943
BccI CCATC 7 cut(s) 317, 575, 788, 1313, 1322, 1618, 1877
BceAI ACGGC 5 cut(s) 217, 297, 382, 390, 794
BciT130I CCWGG 6 cut(s) 453, 506, 816, 1473, 1494, 2040
BclI TGATCA 3 cut(s) 3, 883, 1621
BcnI CCSGG 1 cut(s) 523
BcoDI GTCTC 4 cut(s) 792, 843, 1879, 2945
BfaI CTAG 4 cut(s) 1266, 2163, 2309, 2324
BfoI RGCGCY 1 cut(s) 1507
BfuAI ACCTGC 4 cut(s) 410, 949, 1447, 2251
BlpI GCTNAGC 1 cut(s) 1205
BmcAI AGTACT 2 cut(s) 1705, 1986
Bme1390I CCNGG 7 cut(s) 453, 506, 523, 816, 1473, 1494, 2040
Bme18I GGWCC 3 cut(s) 256, 1598, 1886
BmeT110I CYCGRG 1 cut(s) 748
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 7 cut(s) 170, 202, 230, 256, 617, 1598, 1886
BmiI GGNNCC 8 cut(s) 231, 257, 418, 712, 1531, 2147, 2808, 2982
BmrFI CCNGG 7 cut(s) 453, 506, 523, 816, 1473, 1494, 2040
BmrI ACTGGG 2 cut(s) 687, 703
BmtI GCTAGC 1 cut(s) 2166
BmuI ACTGGG 2 cut(s) 687, 703
BpiI GAAGAC 1 cut(s) 1943
BpmI CTGGAG 1 cut(s) 805
Bpu1102I GCTNAGC 1 cut(s) 1205
BpuEI CTTGAG 1 cut(s) 2448
BpuMI CCSGG 1 cut(s) 523
Bsa29I ATCGAT 1 cut(s) 1439
BsaAI YACGTR 1 cut(s) 500
BsaBI GATNNNNATC 1 cut(s) 978
BsaHI GRCGYC 1 cut(s) 369
BsaI GGTCTC 1 cut(s) 2945
BsaJI CCNNGG 4 cut(s) 504, 815, 906, 2100
BsaXI ACNNNNNCTCC 2 cut(s) 1544, 1574
Bse1I ACTGG 8 cut(s) 169, 620, 682, 698, 1062, 2276, 2406, 2880
Bse3DI GCAATG 3 cut(s) 1057, 1171, 2721
Bse8I GATNNNNATC 1 cut(s) 978
BseBI CCWGG 6 cut(s) 453, 506, 816, 1473, 1494, 2040
BseCI ATCGAT 1 cut(s) 1439
BseDI CCNNGG 4 cut(s) 504, 815, 906, 2100
BseGI GGATG 9 cut(s) 309, 979, 1309, 1324, 1921, 2312, 2410, 2722, 2890
BseJI GATNNNNATC 1 cut(s) 978
BseMI GCAATG 3 cut(s) 1057, 1171, 2721
BseMII CTCAG 2 cut(s) 400, 2967
BseNI ACTGG 8 cut(s) 169, 620, 682, 698, 1062, 2276, 2406, 2880
BseRI GAGGAG 5 cut(s) 277, 280, 338, 1205, 3009
Bsh1236I CGCG 3 cut(s) 363, 1378, 1596
BshFI GGCC 8 cut(s) 172, 203, 232, 266, 284, 619, 662, 1279
BshNI GGYRCC 1 cut(s) 2980
BshVI ATCGAT 1 cut(s) 1439
BsiHKCI CYCGRG 1 cut(s) 748
BsiSI CCGG 3 cut(s) 414, 522, 2946
BslFI GGGAC 4 cut(s) 269, 538, 1348, 2533
BsmAI GTCTC 4 cut(s) 792, 843, 1879, 2945
BsmFI GGGAC 4 cut(s) 269, 538, 1348, 2533
BsmI GAATGC 2 cut(s) 1463, 2215
BsnI GGCC 8 cut(s) 172, 203, 232, 266, 284, 619, 662, 1279
Bso31I GGTCTC 1 cut(s) 2945
BsoBI CYCGRG 1 cut(s) 748
Bsp143I GATC 9 cut(s) 3, 640, 757, 883, 979, 1445, 1621, 2320, 2989
Bsp1720I GCTNAGC 1 cut(s) 1205
BspANI GGCC 8 cut(s) 172, 203, 232, 266, 284, 619, 662, 1279
BspCNI CTCAG 2 cut(s) 399, 2968
BspDI ATCGAT 1 cut(s) 1439
BspFNI CGCG 3 cut(s) 363, 1378, 1596
BspHI TCATGA 2 cut(s) 1582, 3042
BspLI GGNNCC 8 cut(s) 231, 257, 418, 712, 1531, 2147, 2808, 2982
BspMI ACCTGC 4 cut(s) 410, 949, 1447, 2251
BspOI GCTAGC 1 cut(s) 2166
BspPI GGATC 1 cut(s) 635
BspT107I GGYRCC 1 cut(s) 2980
BspTNI GGTCTC 1 cut(s) 2945
BsrDI GCAATG 3 cut(s) 1057, 1171, 2721
BsrI ACTGG 8 cut(s) 169, 620, 682, 698, 1062, 2276, 2406, 2880
BssECI CCNNGG 4 cut(s) 504, 815, 906, 2100
BssMI GATC 9 cut(s) 3, 640, 757, 883, 979, 1445, 1621, 2320, 2989
BssNI GRCGYC 1 cut(s) 369
BssSI CACGAG 2 cut(s) 214, 2534
BssT1I CCWWGG 2 cut(s) 906, 2100
Bst2BI CACGAG 2 cut(s) 214, 2534
Bst2UI CCWGG 6 cut(s) 453, 506, 816, 1473, 1494, 2040
Bst4CI ACNGT 6 cut(s) 132, 531, 577, 1391, 2025, 2590
Bst6I CTCTTC 4 cut(s) 1182, 1268, 1308, 2159
BstACI GRCGYC 1 cut(s) 369
BstAPI GCANNNNNTGC 2 cut(s) 2235, 2266
BstBAI YACGTR 1 cut(s) 500
BstC8I GCNNGC 5 cut(s) 264, 2009, 2108, 2164, 2215
BstDEI CTNAG 7 cut(s) 386, 779, 1155, 1205, 2015, 2111, 2976
BstEII GGTNACC 1 cut(s) 915
BstENI CCTNNNNNAGG 1 cut(s) 2016
BstF5I GGATG 9 cut(s) 309, 979, 1309, 1324, 1921, 2312, 2410, 2722, 2890
BstFNI CGCG 3 cut(s) 363, 1378, 1596
BstH2I RGCGCY 1 cut(s) 1507
BstHHI GCGC 2 cut(s) 1378, 1506
BstKTI GATC 9 cut(s) 6, 643, 760, 886, 982, 1448, 1624, 2323, 2992
BstMAI GTCTC 4 cut(s) 792, 843, 1879, 2945
BstMBI GATC 9 cut(s) 3, 640, 757, 883, 979, 1445, 1621, 2320, 2989
BstMWI GCNNNNNNNGC 6 cut(s) 360, 1285, 1512, 2235, 2266, 2921
BstNI CCWGG 6 cut(s) 453, 506, 816, 1473, 1494, 2040
BstNSI RCATGY 2 cut(s) 1525, 1871
BstPI GGTNACC 1 cut(s) 915
BstSCI CCNGG 7 cut(s) 451, 504, 521, 814, 1471, 1492, 2038
BstSNI TACGTA 1 cut(s) 500
BstUI CGCG 3 cut(s) 363, 1378, 1596
BstV2I GAAGAC 1 cut(s) 1943
Bsu15I ATCGAT 1 cut(s) 1439
BsuRI GGCC 8 cut(s) 172, 203, 232, 266, 284, 619, 662, 1279
BsuTUI ATCGAT 1 cut(s) 1439
BtrI CACGTC 1 cut(s) 274
BtsCI GGATG 9 cut(s) 309, 979, 1309, 1324, 1921, 2312, 2410, 2722, 2890
BveI ACCTGC 4 cut(s) 410, 949, 1447, 2251
Cac8I GCNNGC 5 cut(s) 264, 2009, 2108, 2164, 2215
CaiI CAGNNNCTG 1 cut(s) 542
CciI TCATGA 2 cut(s) 1582, 3042
CfoI GCGC 2 cut(s) 1378, 1506
Cfr13I GGNCC 7 cut(s) 170, 202, 230, 256, 617, 1598, 1886
ClaI ATCGAT 1 cut(s) 1439
CseI GACGC 3 cut(s) 358, 1367, 2481
Csp6I GTAC 7 cut(s) 238, 501, 776, 1704, 1985, 2254, 2981
CspCI CAANNNNNGTGG 2 cut(s) 2413, 2448
CviQI GTAC 7 cut(s) 238, 501, 776, 1704, 1985, 2254, 2981
DdeI CTNAG 7 cut(s) 386, 779, 1155, 1205, 2015, 2111, 2976
DpnI GATC 9 cut(s) 5, 642, 759, 885, 981, 1447, 1623, 2322, 2991
DpnII GATC 9 cut(s) 3, 640, 757, 883, 979, 1445, 1621, 2320, 2989
DraI TTTAAA 1 cut(s) 127
DraIII CACNNNGTG 1 cut(s) 277
DrdI GACNNNNNNGTC 1 cut(s) 431
DseDI GACNNNNNNGTC 1 cut(s) 431
Eam1104I CTCTTC 4 cut(s) 1182, 1268, 1308, 2159
EarI CTCTTC 4 cut(s) 1182, 1268, 1308, 2159
EciI GGCGGA 2 cut(s) 341, 2894
Eco105I TACGTA 1 cut(s) 500
Eco130I CCWWGG 2 cut(s) 906, 2100
Eco147I AGGCCT 1 cut(s) 266
Eco31I GGTCTC 1 cut(s) 2945
Eco47I GGWCC 3 cut(s) 256, 1598, 1886
Eco57I CTGAAG 3 cut(s) 1152, 2140, 2615
Eco88I CYCGRG 1 cut(s) 748
Eco91I GGTNACC 1 cut(s) 915
EcoNI CCTNNNNNAGG 1 cut(s) 2016
EcoO65I GGTNACC 1 cut(s) 915
EcoRII CCWGG 6 cut(s) 451, 504, 814, 1471, 1492, 2038
EcoT14I CCWWGG 2 cut(s) 906, 2100
EcoT22I ATGCAT 2 cut(s) 1297, 2477
ErhI CCWWGG 2 cut(s) 906, 2100
FalI AAGNNNNNCTT 2 cut(s) 1230, 1262
FaqI GGGAC 4 cut(s) 269, 538, 1348, 2533
FauI CCCGC 2 cut(s) 218, 2265
FauNDI CATATG 1 cut(s) 1297
FbaI TGATCA 3 cut(s) 3, 883, 1621
FblI GTMKAC 1 cut(s) 423
FokI GGATG 9 cut(s) 296, 986, 1316, 1331, 1928, 2299, 2417, 2729, 2897
FspBI CTAG 4 cut(s) 1266, 2163, 2309, 2324
GlaI GCGC 2 cut(s) 1377, 1505
GsuI CTGGAG 1 cut(s) 805
HaeII RGCGCY 1 cut(s) 1507
HaeIII GGCC 8 cut(s) 172, 203, 232, 266, 284, 619, 662, 1279
HapII CCGG 3 cut(s) 414, 522, 2946
HgaI GACGC 3 cut(s) 358, 1367, 2481
HhaI GCGC 2 cut(s) 1378, 1506
Hin1I GRCGYC 1 cut(s) 369
Hin6I GCGC 2 cut(s) 1376, 1504
HinP1I GCGC 2 cut(s) 1376, 1504
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 6 cut(s) 563, 2442, 2483, 2513, 2540, 2579
HpaII CCGG 3 cut(s) 414, 522, 2946
HphI GGTGA 8 cut(s) 44, 169, 557, 893, 909, 1247, 2275, 2944
Hpy166II GTNNAC 5 cut(s) 45, 424, 1385, 2045, 2817
Hpy188I TCNGA 7 cut(s) 197, 295, 468, 984, 2320, 2595, 2977
Hpy8I GTNNAC 5 cut(s) 45, 424, 1385, 2045, 2817
Hpy99I CGWCG 6 cut(s) 238, 278, 368, 425, 1383, 3027
HpyCH4III ACNGT 6 cut(s) 132, 531, 577, 1391, 2025, 2590
HpyCH4IV ACGT 2 cut(s) 273, 499
HpyF10VI GCNNNNNNNGC 6 cut(s) 360, 1285, 1512, 2235, 2266, 2921
HpyF3I CTNAG 7 cut(s) 386, 779, 1155, 1205, 2015, 2111, 2976
HpySE526I ACGT 2 cut(s) 273, 499
Hsp92I GRCGYC 1 cut(s) 369
HspAI GCGC 2 cut(s) 1376, 1504
KpnI GGTACC 1 cut(s) 2984
Ksp22I TGATCA 3 cut(s) 3, 883, 1621
Kzo9I GATC 9 cut(s) 3, 640, 757, 883, 979, 1445, 1621, 2320, 2989
MaeI CTAG 4 cut(s) 1266, 2163, 2309, 2324
MaeII ACGT 2 cut(s) 273, 499
MalI GATC 9 cut(s) 5, 642, 759, 885, 981, 1447, 1623, 2322, 2991
MboI GATC 9 cut(s) 3, 640, 757, 883, 979, 1445, 1621, 2320, 2989
MfeI CAATTG 2 cut(s) 1542, 1945
MlyI GAGTC 6 cut(s) 311, 796, 1099, 1370, 1831, 2029
MmeI TCCRAC 3 cut(s) 318, 1338, 2830
Mph1103I ATGCAT 2 cut(s) 1297, 2477
MroXI GAANNNNTTC 1 cut(s) 1137
MspA1I CMGCKG 3 cut(s) 222, 539, 857
MspI CCGG 3 cut(s) 414, 522, 2946
MspR9I CCNGG 7 cut(s) 453, 506, 523, 816, 1473, 1494, 2040
MunI CAATTG 2 cut(s) 1542, 1945
Mva1269I GAATGC 2 cut(s) 1463, 2215
MvaI CCWGG 6 cut(s) 453, 506, 816, 1473, 1494, 2040
MvnI CGCG 3 cut(s) 363, 1378, 1596
MwoI GCNNNNNNNGC 6 cut(s) 360, 1285, 1512, 2235, 2266, 2921
NciI CCSGG 1 cut(s) 523
NdeI CATATG 1 cut(s) 1297
NdeII GATC 9 cut(s) 3, 640, 757, 883, 979, 1445, 1621, 2320, 2989
NheI GCTAGC 1 cut(s) 2162
NlaIV GGNNCC 8 cut(s) 231, 257, 418, 712, 1531, 2147, 2808, 2982
NsiI ATGCAT 2 cut(s) 1297, 2477
NspI RCATGY 2 cut(s) 1525, 1871
PacI TTAATTAA 1 cut(s) 3057
PagI TCATGA 2 cut(s) 1582, 3042
PaqCI CACCTGC 3 cut(s) 410, 1447, 2251
PceI AGGCCT 1 cut(s) 266
PciI ACATGT 1 cut(s) 1867
PcsI WCGNNNNNNNCGW 3 cut(s) 312, 429, 2912
PctI GAATGC 2 cut(s) 1463, 2215
PdmI GAANNNNTTC 1 cut(s) 1137
PfeI GAWTC 7 cut(s) 483, 671, 1097, 1253, 1557, 2096, 2380
PflFI GACNNNGTC 1 cut(s) 368
PfoI TCCNGGA 1 cut(s) 521
PleI GAGTC 6 cut(s) 311, 795, 1099, 1369, 1830, 2029
PpsI GAGTC 6 cut(s) 311, 795, 1099, 1369, 1830, 2029
Ppu21I YACGTR 1 cut(s) 500
PscI ACATGT 1 cut(s) 1867
PshBI ATTAAT 1 cut(s) 3053
Psp6I CCWGG 6 cut(s) 451, 504, 814, 1471, 1492, 2038
PspEI GGTNACC 1 cut(s) 915
PspGI CCWGG 6 cut(s) 451, 504, 814, 1471, 1492, 2038
PspN4I GGNNCC 8 cut(s) 231, 257, 418, 712, 1531, 2147, 2808, 2982
PspPI GGNCC 7 cut(s) 170, 202, 230, 256, 617, 1598, 1886
PstNI CAGNNNCTG 1 cut(s) 542
PsyI GACNNNGTC 1 cut(s) 368
PvuII CAGCTG 2 cut(s) 539, 857
RsaI GTAC 7 cut(s) 239, 502, 777, 1705, 1986, 2255, 2982
RsaNI GTAC 7 cut(s) 238, 501, 776, 1704, 1985, 2254, 2981
SalI GTCGAC 1 cut(s) 422
Sau3AI GATC 9 cut(s) 3, 640, 757, 883, 979, 1445, 1621, 2320, 2989
Sau96I GGNCC 7 cut(s) 170, 202, 230, 256, 617, 1598, 1886
ScaI AGTACT 2 cut(s) 1705, 1986
SchI GAGTC 6 cut(s) 311, 796, 1099, 1370, 1831, 2029
ScrFI CCNGG 7 cut(s) 453, 506, 523, 816, 1473, 1494, 2040
SinI GGWCC 3 cut(s) 256, 1598, 1886
SmlI CTYRAG 1 cut(s) 2463
SmoI CTYRAG 1 cut(s) 2463
SnaBI TACGTA 1 cut(s) 500
SseBI AGGCCT 1 cut(s) 266
SspI AATATT 3 cut(s) 90, 159, 656
SspMI CTAG 4 cut(s) 1266, 2163, 2309, 2324
StuI AGGCCT 1 cut(s) 266
StyD4I CCNGG 7 cut(s) 451, 504, 521, 814, 1471, 1492, 2038
StyI CCWWGG 2 cut(s) 906, 2100
TaaI ACNGT 6 cut(s) 132, 531, 577, 1391, 2025, 2590
TaiI ACGT 2 cut(s) 276, 502
TaqI TCGA 8 cut(s) 106, 423, 639, 756, 1439, 2553, 2992, 3022
TatI WGTACW 3 cut(s) 775, 1703, 1984
TauI GCSGC 2 cut(s) 225, 2732
TfiI GAWTC 7 cut(s) 483, 671, 1097, 1253, 1557, 2096, 2380
TspGWI ACGGA 6 cut(s) 409, 421, 939, 1569, 2520, 2729
Tth111I GACNNNGTC 1 cut(s) 368
VpaK11BI GGWCC 3 cut(s) 256, 1598, 1886
VspI ATTAAT 1 cut(s) 3053
XagI CCTNNNNNAGG 1 cut(s) 2016
XapI RAATTY 3 cut(s) 461, 1976, 2293
XceI RCATGY 2 cut(s) 1525, 1871
XcmI CCANNNNNNNNNTGG 1 cut(s) 1728
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 1137
XspI CTAG 4 cut(s) 1266, 2163, 2309, 2324
ZrmI AGTACT 2 cut(s) 1705, 1986
Zsp2I ATGCAT 2 cut(s) 1297, 2477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.