pycom14g18070

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009559
Physical Location & Seq
Reverse (-)
19841676 .. 19843084
1409 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGGGGTGCACATTCTCTGGTTTGGGCGCTCTGTACGACGCCGTGAACGGCGGAGGCGATGTGTGGATTAACGAGAATCGGTTCAAGATCGTGAGGCAGCTCGGGGAAGGTGGGTTCGCTTATGTGTTTTTGGTCAAGGAGGTGGTCGCCGACTCCTCCGCCTCCGGCGGTGGTGGTCTCGCCAAGAAATTCAAGGATCCCTCTCATGTCTCAGATGATGGAACTTATGCTATGAAAAAAGTTCTCATTCAGAATAGTGAGCAGTTGGAGTTGGTGAAGGAAGAGATCCGTGTTTCTTCGCTGTTCAGTCATCCCAATCTGCTTCCGCTTCTTGATCATGCCATTATTGCCGTTAAGACAACACAAGAACAATCTTGGAACCATGAAGCATACTTGTTATTTCCAGTTCATCTGGATGGAACTTTACTGGACAATGCCAAGACTATGAAAGCTAAAAAGGAGTTCTTTTCCACCTCAGATGTTCTTCAAATATTCCGGCAGCTTTGTGCAGGACTTAAGCATATGCACACTTTGGATCCACCAACTATGCACATAATGATATCAAACCTGGTAACGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

194

Amino Acids

21.3

Weight (kDa)

6.22

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 68 - 177 5.2e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 51, 159, 168, 326
AclI AACGTT 1 cut(s) 575
AclWI GGATC 5 cut(s) 191, 204, 280, 530, 543
AcsI RAATTY 1 cut(s) 188
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 1 cut(s) 35
AflII CTTAAG 1 cut(s) 515
AgsI TTSAA 3 cut(s) 85, 193, 488
AjnI CCWGG 1 cut(s) 567
AloI GAACNNNNNNTCC 2 cut(s) 98, 130
AluBI AGCT 3 cut(s) 100, 452, 502
AluI AGCT 3 cut(s) 100, 452, 502
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 2 cut(s) 182, 214
Alw44I GTGCAC 1 cut(s) 7
AlwI GGATC 5 cut(s) 191, 204, 280, 530, 543
Ama87I CYCGRG 1 cut(s) 101
ApaLI GTGCAC 1 cut(s) 7
ApeKI GCWGC 2 cut(s) 97, 499
ApoI RAATTY 1 cut(s) 188
Asp700I GAANNNNTTC 1 cut(s) 80
AspLEI GCGC 1 cut(s) 29
AsuHPI GGTGA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 101
BaeGI GKGCMC 1 cut(s) 11
BamHI GGATCC 2 cut(s) 196, 535
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 2 cut(s) 109, 511
BccI CCATC 2 cut(s) 212, 410
BceAI ACGGC 3 cut(s) 26, 64, 335
BciT130I CCWGG 1 cut(s) 569
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 182, 214
BfoI RGCGCY 1 cut(s) 30
BfrI CTTAAG 1 cut(s) 515
BisI GCNGC 2 cut(s) 98, 500
BlsI GCNGC 2 cut(s) 99, 501
Bme1390I CCNGG 1 cut(s) 569
BmeT110I CYCGRG 1 cut(s) 101
BmiI GGNNCC 3 cut(s) 198, 380, 537
BmrFI CCNGG 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bse1I ACTGG 2 cut(s) 404, 432
BseBI CCWGG 1 cut(s) 569
BseGI GGATG 2 cut(s) 310, 421
BseMII CTCAG 2 cut(s) 225, 489
BseNI ACTGG 2 cut(s) 404, 432
BseRI GAGGAG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 11
BseXI GCAGC 2 cut(s) 109, 511
BsgI GTGCAG 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 101
BsiSI CCGG 2 cut(s) 165, 496
BsmAI GTCTC 2 cut(s) 182, 214
Bso31I GGTCTC 1 cut(s) 182
BsoBI CYCGRG 1 cut(s) 101
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 5 cut(s) 87, 196, 285, 334, 535
BspACI CCGC 4 cut(s) 51, 159, 168, 326
BspCNI CTCAG 2 cut(s) 224, 488
BspLI GGNNCC 3 cut(s) 198, 380, 537
BspPI GGATC 5 cut(s) 191, 204, 280, 530, 543
BspTI CTTAAG 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 182
BsrI ACTGG 2 cut(s) 404, 432
BssMI GATC 5 cut(s) 87, 196, 285, 334, 535
BssNI GRCGYC 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 569
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 515
BstDEI CTNAG 2 cut(s) 211, 475
BstF5I GGATG 2 cut(s) 310, 421
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 5 cut(s) 90, 199, 288, 337, 538
BstMAI GTCTC 2 cut(s) 182, 214
BstMBI GATC 5 cut(s) 87, 196, 285, 334, 535
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNI CCWGG 1 cut(s) 569
BstSCI CCNGG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 109, 511
BstX2I RGATCY 3 cut(s) 196, 285, 535
BstYI RGATCY 3 cut(s) 196, 285, 535
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 310, 421
CfoI GCGC 1 cut(s) 29
CseI GACGC 1 cut(s) 47
CsiI ACCWGGT 1 cut(s) 567
Csp6I GTAC 1 cut(s) 34
CviAII CATG 3 cut(s) 206, 338, 383
CviJI RGCY 3 cut(s) 100, 452, 502
CviKI_1 RGCY 3 cut(s) 100, 452, 502
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 2 cut(s) 211, 475
DpnI GATC 5 cut(s) 89, 198, 287, 336, 537
DpnII GATC 5 cut(s) 87, 196, 285, 334, 535
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 2 cut(s) 66, 148
Eco31I GGTCTC 1 cut(s) 182
Eco32I GATATC 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 567
EcoRV GATATC 1 cut(s) 561
FaeI CATG 3 cut(s) 209, 341, 386
FalI AAGNNNNNCTT 2 cut(s) 449, 481
FatI CATG 3 cut(s) 205, 337, 382
FauNDI CATATG 1 cut(s) 522
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 98, 500
FokI GGATG 2 cut(s) 297, 428
Fsp4HI GCNGC 2 cut(s) 98, 500
GlaI GCGC 1 cut(s) 28
GluI GCNGC 2 cut(s) 98, 500
HaeII RGCGCY 1 cut(s) 30
HapII CCGG 2 cut(s) 165, 496
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 29
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 3 cut(s) 209, 341, 386
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HinfI GANTC 2 cut(s) 76, 152
HpaII CCGG 2 cut(s) 165, 496
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 2 cut(s) 9, 46
Hpy188I TCNGA 4 cut(s) 214, 252, 478, 581
Hpy188III TCNNGA 4 cut(s) 85, 91, 332, 413
Hpy8I GTNNAC 2 cut(s) 9, 46
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 2 cut(s) 101, 271
HpyCH4IV ACGT 1 cut(s) 575
HpyCH4V TGCA 4 cut(s) 9, 509, 526, 550
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 2 cut(s) 211, 475
HpySE526I ACGT 1 cut(s) 575
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 3 cut(s) 209, 341, 386
HspAI GCGC 1 cut(s) 27
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 5 cut(s) 87, 196, 285, 334, 535
LpnPI CCDG 8 cut(s) 3, 178, 398, 413, 417, 495, 509, 554
Lsp1109I GCAGC 2 cut(s) 109, 511
MabI ACCWGGT 1 cut(s) 567
MaeII ACGT 1 cut(s) 575
MaeIII GTNAC 1 cut(s) 571
MalI GATC 5 cut(s) 89, 198, 287, 336, 537
MboI GATC 5 cut(s) 87, 196, 285, 334, 535
MboII GAAGA 3 cut(s) 288, 293, 476
MflI RGATCY 3 cut(s) 196, 285, 535
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 1 cut(s) 188
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 7 cut(s) 47, 87, 133, 166, 172, 211, 484
MroXI GAANNNNTTC 1 cut(s) 80
MseI TTAA 3 cut(s) 69, 354, 516
MslI CAYNNNNRTG 1 cut(s) 414
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 2 cut(s) 165, 496
MspR9I CCNGG 1 cut(s) 569
MvaI CCWGG 1 cut(s) 569
MwoI GCNNNNNNNGC 1 cut(s) 347
NdeI CATATG 1 cut(s) 522
NdeII GATC 5 cut(s) 87, 196, 285, 334, 535
NlaIII CATG 3 cut(s) 209, 341, 386
NlaIV GGNNCC 3 cut(s) 198, 380, 537
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 76
PkrI GCNGC 2 cut(s) 99, 501
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
Psp1406I AACGTT 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 567
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 3 cut(s) 198, 380, 537
PsuI RGATCY 3 cut(s) 196, 285, 535
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 1 cut(s) 414
SaqAI TTAA 3 cut(s) 69, 354, 516
SatI GCNGC 2 cut(s) 98, 500
Sau3AI GATC 5 cut(s) 87, 196, 285, 334, 535
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 569
SduI GDGCHC 1 cut(s) 11
SetI ASST 8 cut(s) 102, 112, 144, 454, 476, 504, 570, 578
SexAI ACCWGGT 1 cut(s) 567
SmiMI CAYNNNNRTG 1 cut(s) 414
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 1 cut(s) 188
SsiI CCGC 4 cut(s) 51, 159, 168, 326
SspI AATATT 1 cut(s) 492
StyD4I CCNGG 1 cut(s) 567
TaiI ACGT 1 cut(s) 578
TasI AATT 1 cut(s) 188
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 3 cut(s) 69, 354, 516
Tru9I TTAA 3 cut(s) 69, 354, 516
TseI GCWGC 2 cut(s) 97, 499
TspDTI ATGAA 4 cut(s) 248, 398, 399, 461
TspGWI ACGGA 1 cut(s) 278
Vha464I CTTAAG 1 cut(s) 515
VneI GTGCAC 1 cut(s) 7
XapI RAATTY 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.