Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0009559

Physical Location & Seq

Genomic Coordinates Forward (+)

58830088 .. 58834150

Gene Structure 4063 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0462600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGCTCCTCCATCTCTCTACTCTCCTCCGCGCCATCCATTCTACCCGCCCAATGGCGCGTTACATCCCACGCGCCACCCTCATATCCATCACCCCCATTCCCATCCGAAACACCACCCCCAGACGGAATTCAATCGTCCCTCCCCAACCGCACCGTTTCGACGCCTCCAAAGCCTCGCTCTTCTTCGGCCGGAGATTCCACGCCCTCTGCGGCGGCGACTGGAGAGCTCGTCCGAGACCGTCGTTACGCATCTGTGCTGCGCAGAGAGACTACCGGAAAGTGAGGCGGCGAGCCCCGAAGAGAAAGGACAAGGAGCTCGAGCTCAACGTCAGCATTTGCATCGAAGAAGAGTTGCCTGACGATCCTGAAATCCTTGGGATAGCGGAGTTGCTCAGGATTAATGTTCCGATGGCAATGAAGCTAGCGTTTGATGGCTTGAAAGATTCGGAGTATAAAACCAGGGACGATGGCATCAGTGACGTCGGAGGGTTTCAGAGCGTGGAGCTTTCGGTGATGCTTTGCAATGACGAGTTTATCCGAAAGCTTAACAAAGAATGGAGAGATGAGGACCATGCTACTGATGTGCTTTCAATGTCACAACATGTCCCTGAGCTTAGGCTTCCCATTCTTATGTTGGGTGACGTTGTAATTTCTGTTGAGACTGCGGCGAGACAAGCAGAAGAAAGAGGACATACGATTCTTGACGAGATACGTATTCTCATGATACATGGATTGCTGCATCTACTAGGATTTGATCATGAGATTAGCGAAGAAGCCGAAGTGGAAATGGAGAAGGAGGAGGAGCTTCTCTTGAAGAGCCTTGGGTGGACGGGAAAAGGATTGATTCAGAGTGCGTCCAATGCTGAAATCAATGCAATTTCCCATGAGGAGAAACAGGATGACAGGAAGAGAGAAGGCAGCCTTCGATACTATAAACCAAAGTTCAAATATATATTCTGCGATATGGATGGCACCCTTCTCAACAGTAAAACTCAAATCAGTTCAACAACTGCAAAGGCTCTTAAAGAGGTCTCATCAAGGGGTGTGAAAATAGTGATAGCAACTGGAAAAACTCGCTCAGCTGTGATCAACATTTTTAAAATGGTAGACTTAGCAGGAAAAGATGGCATTGTTTCAGAATTTTCTCCTGGGGTTTTCTGCCAGGGATTGCTTGTTTATGGTAAGCAAGGTCGGGAAATCTTTAAAGGGAATTTAGATCCAAATGTCTGCAGAGAGGCGTGTCTTTACTCTTGGGAGAATAGGATACCTCTTATTGCTTACAGTGAGGATCGTTGCTTCACTCTATTTGACCACCCACTAGTTGACTCACTACATACTGTATATCATGAGCCAAAGGGGGAGATCATGCCTTCAGTTGAGCATCTCTTGGCTGCTGGTGACATACAGAAAGTAATCTTCATGGACACTGCCGAAGGAGTCGCAACTACTTTGAGGCCACACTGGTCAGGAGCAATTAGAGATCGTGCAGCAGTTGTTCAAGCTCAGCCGGACATGCTTGAAATTGTCCCTCCGGGGACCTCAAAAGGGAGTGGAGTAAAATTGCTGCTTGATCATTTGGGCATCACTCCAAAGGAGATCATGGCTATTGGCGATGGAGAAAATGACATTGAGATGCTTGAACTAGCTTCTTTAGGTGTTGCTCTTAGCAATGGTTCAGAGAAAACAAAAGCTGTGGCTAATTTAATTGGTGCCAGTAACGACGAAGATGGTGCAGCTGATGCGATCTACCGGTATGCATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009719 GO:0009987 GO:0010033 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050896 GO:0051716 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071559 GO:0071560 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

587

Amino Acids

65.5

Weight (kDa)

5.99

Isoelectric Point (pI)

38.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
YbeY	PF02130	165 - 273	3.8e-32	Endoribonuclease YbeY
Hydrolase_3	PF08282	318 - 582	6.5e-53	haloacid dehalogenase-like hydrolase
S6PP	PF05116	498 - 561	4.4e-09	Sucrose-6F-phosphate phosphohydrolase
Hydrolase	PF00702	507 - 549	2.2e-06	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009559)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08160 AT5G08160
fragaria_vesca	FvH4_5g13060
malus_domestica	MD06G1207400.v1.1 MD14G1217900.v1.1
prunus_persica	Prupe.5G211800_v2.0.a1 Prupe.5G211800_v2.0.a1 Prupe.5G211800_v2.0.a1
pyrus_communis	pycom06g18530 pycom14g18070
rosa_chinensis	RchiOBHm_Chr7g0184071
rosa_laevigata	RLG00000005003
rosa_multiflora	Rmu_sc0004712.1_g000009 Rmu_sc0007533.1_g000019
rosa_roxburghii	Rroxscaffold_3G00269910
rosa_rugosa	Rorug06G0462500 Rorug06G0462600
rosa_samantha	Rh7AG083100 Rh7BG063900 Rh7CG064600 Rh7DG064100
rosa_wichuraiana	Rw7G005250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	483
Acc16I	TGCGCA	1 cut(s)	261
AccB1I	GGYRCC	2 cut(s)	971, 1709
AccI	GTMKAC	1 cut(s)	1107
AccII	CGCG	3 cut(s)	30, 58, 72
AciI	CCGC	8 cut(s)	28, 46, 149, 210, 213, 286, 383, 665
AclWI	GGATC	3 cut(s)	356, 1211, 1296
AcoI	YGGCCR	1 cut(s)	187
AcsI	RAATTY	3 cut(s)	127, 1139, 1210
AcuI	CTGAAG	1 cut(s)	1356
AcyI	GRCGYC	2 cut(s)	162, 480
AfiI	CCNNNNNNNGG	3 cut(s)	52, 123, 1545
AflIII	ACRYGT	1 cut(s)	601
AgeI	ACCGGT	1 cut(s)	1749
AgsI	TTSAA	9 cut(s)	132, 439, 591, 814, 946, 1005, 1499, 1520, 1640
AhlI	ACTAGT	1 cut(s)	1318
AjnI	CCWGG	3 cut(s)	458, 1147, 1161
Alw21I	GWGCWC	3 cut(s)	229, 318, 324
Alw26I	GTCTC	5 cut(s)	229, 261, 653, 664, 1036
AlwI	GGATC	3 cut(s)	356, 1211, 1296
Ama87I	CYCGRG	1 cut(s)	317
AoxI	GGCC	2 cut(s)	187, 1454
ApeKI	GCWGC	7 cut(s)	257, 736, 918, 1391, 1487, 1564, 1733
ApoI	RAATTY	3 cut(s)	127, 1139, 1210
AseI	ATTAAT	1 cut(s)	399
AsiGI	ACCGGT	1 cut(s)	1749
AspLEI	GCGC	4 cut(s)	32, 58, 74, 262
AspS9I	GGNCC	2 cut(s)	568, 1536
AsuC2I	CCSGG	1 cut(s)	1533
AsuHPI	GGTGA	4 cut(s)	82, 523, 650, 1409
AsuNHI	GCTAGC	1 cut(s)	421
AvaI	CYCGRG	1 cut(s)	317
AvaII	GGWCC	2 cut(s)	568, 1536
BanI	GGYRCC	2 cut(s)	971, 1709
BanII	GRGCYC	4 cut(s)	229, 295, 318, 324
Bbv12I	GWGCWC	3 cut(s)	229, 318, 324
BbvI	GCAGC	7 cut(s)	244, 723, 930, 1378, 1499, 1551, 1745
BcgI	CGANNNNNNTGC	4 cut(s)	322, 356, 1712, 1746
BciT130I	CCWGG	3 cut(s)	460, 1149, 1163
BciVI	GTATCC	1 cut(s)	1257
BclI	TGATCA	3 cut(s)	754, 1086, 1570
BcnI	CCSGG	1 cut(s)	1533
BcoDI	GTCTC	5 cut(s)	229, 261, 653, 664, 1036
BcuI	ACTAGT	1 cut(s)	1318
BfaI	CTAG	4 cut(s)	422, 746, 1319, 1643
BfmI	CTRYAG	1 cut(s)	1228
BfuI	GTATCC	1 cut(s)	1257
BlpI	GCTNAGC	2 cut(s)	1078, 1503
Bme1390I	CCNGG	4 cut(s)	460, 1149, 1163, 1533
Bme18I	GGWCC	2 cut(s)	568, 1536
BmeT110I	CYCGRG	1 cut(s)	317
BmgT120I	GGNCC	2 cut(s)	568, 1536
BmiI	GGNNCC	3 cut(s)	973, 1537, 1711
BmrFI	CCNGG	4 cut(s)	460, 1149, 1163, 1533
BmsI	GCATC	9 cut(s)	258, 348, 480, 504, 748, 1390, 1590, 1623, 1729
BmtI	GCTAGC	1 cut(s)	425
BpmI	CTGGAG	1 cut(s)	241
Bpu10I	CCTNAGC	3 cut(s)	392, 609, 614
Bpu1102I	GCTNAGC	2 cut(s)	1078, 1503
BpuMI	CCSGG	1 cut(s)	1533
BsaAI	YACGTR	1 cut(s)	713
BsaHI	GRCGYC	2 cut(s)	162, 480
BsaI	GGTCTC	2 cut(s)	229, 1036
BsaJI	CCNNGG	6 cut(s)	373, 459, 820, 1148, 1162, 1532
BsaWI	WCCGGW	2 cut(s)	273, 1749
BsaXI	ACNNNNNCTCC	2 cut(s)	214, 244
Bsc4I	CCNNNNNNNGG	3 cut(s)	52, 123, 1545
Bse118I	RCCGGY	1 cut(s)	1749
Bse1I	ACTGG	4 cut(s)	224, 1069, 1466, 1713
Bse3DI	GCAATG	3 cut(s)	420, 529, 1675
BseBI	CCWGG	3 cut(s)	460, 1149, 1163
BseDI	CCNNGG	6 cut(s)	373, 459, 820, 1148, 1162, 1532
BseGI	GGATG	5 cut(s)	33, 63, 102, 904, 973
BseLI	CCNNNNNNNGG	3 cut(s)	52, 123, 1545
BseMI	GCAATG	3 cut(s)	420, 529, 1675
BseMII	CTCAG	4 cut(s)	406, 600, 1092, 1517
BseNI	ACTGG	4 cut(s)	224, 1069, 1466, 1713
BseRI	GAGGAG	4 cut(s)	14, 812, 815, 902
BseX3I	CGGCCG	1 cut(s)	187
BseXI	GCAGC	7 cut(s)	244, 723, 930, 1378, 1499, 1551, 1745
BsgI	GTGCAG	2 cut(s)	1506, 1752
Bsh1236I	CGCG	3 cut(s)	30, 58, 72
Bsh1285I	CGRYCG	1 cut(s)	190
BshFI	GGCC	2 cut(s)	189, 1456
BshNI	GGYRCC	2 cut(s)	971, 1709
BshTI	ACCGGT	1 cut(s)	1749
BsiEI	CGRYCG	1 cut(s)	190
BsiHKAI	GWGCWC	3 cut(s)	229, 318, 324
BsiHKCI	CYCGRG	1 cut(s)	317
BsiSI	CCGG	5 cut(s)	190, 274, 1508, 1532, 1750
BslFI	GGGAC	5 cut(s)	122, 476, 590, 1511, 1549
BslI	CCNNNNNNNGG	3 cut(s)	52, 123, 1545
BsmAI	GTCTC	5 cut(s)	229, 261, 653, 664, 1036
BsmFI	GGGAC	5 cut(s)	122, 476, 590, 1511, 1549
BsnI	GGCC	2 cut(s)	189, 1456
Bso31I	GGTCTC	2 cut(s)	229, 1036
BsoBI	CYCGRG	1 cut(s)	317
Bsp1286I	GDGCHC	4 cut(s)	229, 295, 318, 324
Bsp1720I	GCTNAGC	2 cut(s)	1078, 1503
BspACI	CCGC	8 cut(s)	28, 46, 149, 210, 213, 286, 383, 665
BspANI	GGCC	2 cut(s)	189, 1456
BspCNI	CTCAG	4 cut(s)	405, 601, 1091, 1516
BspFNI	CGCG	3 cut(s)	30, 58, 72
BspHI	TCATGA	3 cut(s)	720, 757, 1345
BspLI	GGNNCC	3 cut(s)	973, 1537, 1711
BspMAI	CTGCAG	1 cut(s)	1232
BspOI	GCTAGC	1 cut(s)	425
BspPI	GGATC	3 cut(s)	356, 1211, 1296
BspQI	GCTCTTC	2 cut(s)	185, 809
BspT107I	GGYRCC	2 cut(s)	971, 1709
BspTNI	GGTCTC	2 cut(s)	229, 1036
BsrDI	GCAATG	3 cut(s)	420, 529, 1675
BsrFI	RCCGGY	1 cut(s)	1749
BsrI	ACTGG	4 cut(s)	224, 1069, 1466, 1713
BssAI	RCCGGY	1 cut(s)	1749
BssECI	CCNNGG	6 cut(s)	373, 459, 820, 1148, 1162, 1532
BssNI	GRCGYC	2 cut(s)	162, 480
BssT1I	CCWWGG	2 cut(s)	373, 820
Bst2UI	CCWGG	3 cut(s)	460, 1149, 1163
Bst4CI	ACNGT	5 cut(s)	155, 240, 986, 1283, 1339
Bst6I	CTCTTC	5 cut(s)	185, 293, 342, 809, 902
BstACI	GRCGYC	2 cut(s)	162, 480
BstAPI	GCANNNNNTGC	1 cut(s)	1739
BstBAI	YACGTR	1 cut(s)	713
BstC8I	GCNNGC	2 cut(s)	291, 423
BstDEI	CTNAG	7 cut(s)	392, 609, 614, 1078, 1111, 1503, 1664
BstF5I	GGATG	5 cut(s)	33, 63, 102, 904, 973
BstFNI	CGCG	3 cut(s)	30, 58, 72
BstHHI	GCGC	4 cut(s)	32, 58, 74, 262
BstMAI	GTCTC	5 cut(s)	229, 261, 653, 664, 1036
BstMCI	CGRYCG	1 cut(s)	190
BstMWI	GCNNNNNNNGC	5 cut(s)	170, 674, 860, 1513, 1739
BstNI	CCWGG	3 cut(s)	460, 1149, 1163
BstNSI	RCATGY	2 cut(s)	605, 1516
BstSCI	CCNGG	4 cut(s)	458, 1147, 1161, 1531
BstSFI	CTRYAG	1 cut(s)	1228
BstSNI	TACGTA	1 cut(s)	713
BstUI	CGCG	3 cut(s)	30, 58, 72
BstV1I	GCAGC	7 cut(s)	244, 723, 930, 1378, 1499, 1551, 1745
BstX2I	RGATCY	1 cut(s)	1216
BstYI	RGATCY	1 cut(s)	1216
BstZI	CGGCCG	1 cut(s)	187
BsuI	GTATCC	1 cut(s)	1257
BsuRI	GGCC	2 cut(s)	189, 1456
BtgZI	GCGATG	1 cut(s)	1626
BtsCI	GGATG	5 cut(s)	33, 63, 102, 904, 973
BtsI	GCAGTG	1 cut(s)	1425
BtsIMutI	CAGTG	4 cut(s)	481, 1288, 1425, 1459
Cac8I	GCNNGC	2 cut(s)	291, 423
CciI	TCATGA	3 cut(s)	720, 757, 1345
CfoI	GCGC	4 cut(s)	32, 58, 74, 262
Cfr10I	RCCGGY	1 cut(s)	1749
Cfr13I	GGNCC	2 cut(s)	568, 1536
CseI	GACGC	2 cut(s)	170, 843
CspAI	ACCGGT	1 cut(s)	1749
CspCI	CAANNNNNGTGG	2 cut(s)	1674, 1709
DdeI	CTNAG	7 cut(s)	392, 609, 614, 1078, 1111, 1503, 1664
DraI	TTTAAA	2 cut(s)	1099, 1204
EaeI	YGGCCR	1 cut(s)	187
EagI	CGGCCG	1 cut(s)	187
Eam1104I	CTCTTC	5 cut(s)	185, 293, 342, 809, 902
EarI	CTCTTC	5 cut(s)	185, 293, 342, 809, 902
Ecl136II	GAGCTC	3 cut(s)	227, 316, 322
EclXI	CGGCCG	1 cut(s)	187
Eco105I	TACGTA	1 cut(s)	713
Eco130I	CCWWGG	2 cut(s)	373, 820
Eco24I	GRGCYC	4 cut(s)	229, 295, 318, 324
Eco31I	GGTCTC	2 cut(s)	229, 1036
Eco47I	GGWCC	2 cut(s)	568, 1536
Eco52I	CGGCCG	1 cut(s)	187
Eco53kI	GAGCTC	3 cut(s)	227, 316, 322
Eco57I	CTGAAG	1 cut(s)	1356
Eco88I	CYCGRG	1 cut(s)	317
EcoICRI	GAGCTC	3 cut(s)	227, 316, 322
EcoO109I	RGGNCCY	1 cut(s)	1536
EcoRI	GAATTC	1 cut(s)	127
EcoRII	CCWGG	3 cut(s)	458, 1147, 1161
EcoT14I	CCWWGG	2 cut(s)	373, 820
EcoT22I	ATGCAT	1 cut(s)	1759
EcoT38I	GRGCYC	4 cut(s)	229, 295, 318, 324
ErhI	CCWWGG	2 cut(s)	373, 820
FalI	AAGNNNNNCTT	2 cut(s)	906, 938
FaqI	GGGAC	5 cut(s)	122, 476, 590, 1511, 1549
FauI	CCCGC	1 cut(s)	53
FbaI	TGATCA	3 cut(s)	754, 1086, 1570
FblI	GTMKAC	1 cut(s)	1107
FokI	GGATG	5 cut(s)	20, 50, 89, 911, 980
FriOI	GRGCYC	4 cut(s)	229, 295, 318, 324
FspBI	CTAG	4 cut(s)	422, 746, 1319, 1643
FspI	TGCGCA	1 cut(s)	261
GlaI	GCGC	4 cut(s)	31, 57, 73, 261
GsuI	CTGGAG	1 cut(s)	241
HaeIII	GGCC	2 cut(s)	189, 1456
HapII	CCGG	5 cut(s)	190, 274, 1508, 1532, 1750
HgaI	GACGC	2 cut(s)	170, 843
HhaI	GCGC	4 cut(s)	32, 58, 74, 262
Hin1I	GRCGYC	2 cut(s)	162, 480
Hin6I	GCGC	4 cut(s)	30, 56, 72, 260
HinP1I	GCGC	4 cut(s)	30, 56, 72, 260
HincII	GTYRAC	1 cut(s)	1324
HindII	GTYRAC	1 cut(s)	1324
HindIII	AAGCTT	1 cut(s)	542
HinfI	GANTC	6 cut(s)	195, 443, 697, 844, 1325, 1437
HpaII	CCGG	5 cut(s)	190, 274, 1508, 1532, 1750
HphI	GGTGA	4 cut(s)	82, 523, 650, 1409
Hpy166II	GTNNAC	3 cut(s)	828, 1108, 1324
Hpy188III	TCNNGA	9 cut(s)	365, 394, 701, 721, 758, 811, 1193, 1346, 1467
Hpy8I	GTNNAC	3 cut(s)	828, 1108, 1324
Hpy99I	CGWCG	4 cut(s)	164, 244, 485, 1724
HpyAV	CCTTC	6 cut(s)	787, 908, 932, 986, 1380, 1427
HpyCH4III	ACNGT	5 cut(s)	155, 240, 986, 1283, 1339
HpyCH4IV	ACGT	4 cut(s)	327, 480, 642, 712
HpyCH4V	TGCA	9 cut(s)	339, 522, 739, 875, 1013, 1230, 1487, 1733, 1757
HpyF10VI	GCNNNNNNNGC	5 cut(s)	170, 674, 860, 1513, 1739
HpyF3I	CTNAG	7 cut(s)	392, 609, 614, 1078, 1111, 1503, 1664
HpySE526I	ACGT	4 cut(s)	327, 480, 642, 712
Hsp92I	GRCGYC	2 cut(s)	162, 480
HspAI	GCGC	4 cut(s)	30, 56, 72, 260
Ksp22I	TGATCA	3 cut(s)	754, 1086, 1570
LguI	GCTCTTC	2 cut(s)	185, 809
LmnI	GCTCC	5 cut(s)	9, 313, 502, 802, 1469
Lsp1109I	GCAGC	7 cut(s)	244, 723, 930, 1378, 1499, 1551, 1745
LweI	GCATC	9 cut(s)	258, 348, 480, 504, 748, 1390, 1590, 1623, 1729
MaeI	CTAG	4 cut(s)	422, 746, 1319, 1643
MaeII	ACGT	4 cut(s)	327, 480, 642, 712
MaeIII	GTNAC	7 cut(s)	59, 243, 476, 594, 638, 1397, 1715
MflI	RGATCY	1 cut(s)	1216
MhlI	GDGCHC	4 cut(s)	229, 295, 318, 324
MlyI	GAGTC	2 cut(s)	1319, 1446
MmeI	TCCRAC	1 cut(s)	463
Mph1103I	ATGCAT	1 cut(s)	1759
MseI	TTAA	6 cut(s)	399, 546, 1023, 1098, 1203, 1703
MslI	CAYNNNNRTG	2 cut(s)	629, 1631
MspA1I	CMGCKG	2 cut(s)	1082, 1736
MspI	CCGG	5 cut(s)	190, 274, 1508, 1532, 1750
MspR9I	CCNGG	4 cut(s)	460, 1149, 1163, 1533
MvaI	CCWGG	3 cut(s)	460, 1149, 1163
MvnI	CGCG	3 cut(s)	30, 58, 72
MwoI	GCNNNNNNNGC	5 cut(s)	170, 674, 860, 1513, 1739
NciI	CCSGG	1 cut(s)	1533
NheI	GCTAGC	1 cut(s)	421
NlaIV	GGNNCC	3 cut(s)	973, 1537, 1711
NmuCI	GTSAC	4 cut(s)	476, 594, 638, 1397
NsbI	TGCGCA	1 cut(s)	261
NsiI	ATGCAT	1 cut(s)	1759
NspI	RCATGY	2 cut(s)	605, 1516
PaeR7I	CTCGAG	1 cut(s)	317
PagI	TCATGA	3 cut(s)	720, 757, 1345
PciI	ACATGT	1 cut(s)	601
PciSI	GCTCTTC	2 cut(s)	185, 809
PcsI	WCGNNNNNNNCGW	1 cut(s)	324
PfeI	GAWTC	4 cut(s)	195, 443, 697, 844
PinAI	ACCGGT	1 cut(s)	1749
PleI	GAGTC	2 cut(s)	1319, 1445
PpsI	GAGTC	2 cut(s)	1319, 1445
Ppu21I	YACGTR	1 cut(s)	713
PpuMI	RGGWCCY	1 cut(s)	1536
PscI	ACATGT	1 cut(s)	601
PshBI	ATTAAT	1 cut(s)	399
Psp124BI	GAGCTC	3 cut(s)	229, 318, 324
Psp5II	RGGWCCY	1 cut(s)	1536
Psp6I	CCWGG	3 cut(s)	458, 1147, 1161
PspGI	CCWGG	3 cut(s)	458, 1147, 1161
PspN4I	GGNNCC	3 cut(s)	973, 1537, 1711
PspPI	GGNCC	2 cut(s)	568, 1536
PspPPI	RGGWCCY	1 cut(s)	1536
PspXI	VCTCGAGB	1 cut(s)	317
PstI	CTGCAG	1 cut(s)	1232
PsuI	RGATCY	1 cut(s)	1216
PvuII	CAGCTG	2 cut(s)	1082, 1736
RseI	CAYNNNNRTG	2 cut(s)	629, 1631
SacI	GAGCTC	3 cut(s)	229, 318, 324
SapI	GCTCTTC	2 cut(s)	185, 809
SaqAI	TTAA	6 cut(s)	399, 546, 1023, 1098, 1203, 1703
Sau96I	GGNCC	2 cut(s)	568, 1536
SchI	GAGTC	2 cut(s)	1319, 1446
ScrFI	CCNGG	4 cut(s)	460, 1149, 1163, 1533
SduI	GDGCHC	4 cut(s)	229, 295, 318, 324
SfaNI	GCATC	9 cut(s)	258, 348, 480, 504, 748, 1390, 1590, 1623, 1729
SfcI	CTRYAG	1 cut(s)	1228
Sfr274I	CTCGAG	1 cut(s)	317
SinI	GGWCC	2 cut(s)	568, 1536
SlaI	CTCGAG	1 cut(s)	317
SmiMI	CAYNNNNRTG	2 cut(s)	629, 1631
SmlI	CTYRAG	1 cut(s)	317
SmoI	CTYRAG	1 cut(s)	317
SnaBI	TACGTA	1 cut(s)	713
SpeI	ACTAGT	1 cut(s)	1318
SsiI	CCGC	8 cut(s)	28, 46, 149, 210, 213, 286, 383, 665
SspMI	CTAG	4 cut(s)	422, 746, 1319, 1643
SstI	GAGCTC	3 cut(s)	229, 318, 324
StyD4I	CCNGG	4 cut(s)	458, 1147, 1161, 1531
StyI	CCWWGG	2 cut(s)	373, 820
TaaI	ACNGT	5 cut(s)	155, 240, 986, 1283, 1339
TaiI	ACGT	4 cut(s)	330, 483, 645, 715
TaqI	TCGA	4 cut(s)	159, 318, 342, 925
TauI	GCSGC	4 cut(s)	213, 216, 289, 668
TfiI	GAWTC	4 cut(s)	195, 443, 697, 844
Tru1I	TTAA	6 cut(s)	399, 546, 1023, 1098, 1203, 1703
Tru9I	TTAA	6 cut(s)	399, 546, 1023, 1098, 1203, 1703
TscAI	CASTG	4 cut(s)	481, 1288, 1432, 1466
TseFI	GTSAC	4 cut(s)	476, 594, 638, 1397
TseI	GCWGC	7 cut(s)	257, 736, 918, 1391, 1487, 1564, 1733
Tsp45I	GTSAC	4 cut(s)	476, 594, 638, 1397
TspDTI	ATGAA	2 cut(s)	431, 1408
TspGWI	ACGGA	1 cut(s)	139
TspRI	CASTG	4 cut(s)	481, 1288, 1432, 1466
VpaK11BI	GGWCC	2 cut(s)	568, 1536
VspI	ATTAAT	1 cut(s)	399
XapI	RAATTY	3 cut(s)	127, 1139, 1210
XceI	RCATGY	2 cut(s)	605, 1516
XcmI	CCANNNNNNNNNTGG	1 cut(s)	631
XhoI	CTCGAG	1 cut(s)	317
XmiI	GTMKAC	1 cut(s)	1107
XspI	CTAG	4 cut(s)	422, 746, 1319, 1643
ZraI	GACGTC	1 cut(s)	481
Zsp2I	ATGCAT	1 cut(s)	1759

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.