pycom15g00050

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004467
Physical Location & Seq
Reverse (-)
36789 .. 39276
2488 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1929 bp
ATGGTGATCGATGGAGAGATCAGAGACGAAGGTGAAGGTGGAGGTGGCGGAGGCGGCGGAGGAGGAGGAGGAGAGATCAGAGATGAAACTGCGGAAGGTGTGATTGAATATGTTGCTCAATTCGGAGATTACCGAAGAACGCAGAGGAAAGAATGCTACAACCTGGTGCGGCGTATGAAGCTCTTGCTGCCGCTTTTTCAAGAGACTCGGGAGCTCGATGGGCCAATTCCGGAGAGGGGTGCTGCTTGGTTGGCAAATTTGAAGAAGGTGCTTCTCATGGCAAAGAAATTGTTGAAAACTTGTAATGAGGGGAGCAAAATTTATCTGGCACTTGAAAGTGAGGCAATCATGGTTAGATTTCATGCGGTTCATGAAAGACTCGGTCAGGCTTTAGAAGGTTTGCCTGATGAACTTTGCCTATCAGATGAAGTTGAGGAGCAGGTTGAACTGATGATCATGCAACTTAGACGAGCGAGGAAGCGAACAGATACACAGGACATAGAGCTCGCAATGGATATTATGGTGGTTTTGTCCAAAAAAGAAGATAGAAATGCTGATAGTGCCATTATAGAGAGGCTCGCCCAAAAGCTAGACCTGCAAAAATCCGAGGACCTTAAGATAGAGACAATAGCTGTGAGGAATCTGGTTAAGGATAAAGAGAGAGGAGGGCCAGGTGCAGAGACCATGCAGCAAATCATTGATCTTCTTAACAAATTCAGACAAATCGCAGGAATGGAAGCGACCAATGTCCTTGACAACCCTGTCATGCCTAAAATGCTTGAGAAGTGCCCGTCTTTGATGATCCCTCATGAGTTCCTCTGTCCAATCACGCTAGAAATAATGACGGATCCTGTTATTGTTGCAAGTGGACAGACGTATAACAGAGAAAGCATACAAAAGTGGCTCGACACCAATCATAGGACATGTCCCAAGACCAGGCAGACTCTGGCTCACTTGTCACTTGCACCAAATTATGCTCTAAAAAATCTAATCATGCAGTGGTGTGAGAAGAACAAATTCCAGCTCCTGAAAAAAGAAACTTCCACGGGCCAGGAAAGCTCCTCCACCGAACACAAAGAGGAGATCTCATCATTGGTTGACCAGCTGTTCTCAAGTCACTTAGAAGTGCAAAGGAATGCAGTCATGAAGATTCGTTTGATCTCTAAAGAGAACCCAGAGAACAGAATTTTGGTTGCCAAAAGTGGAGGAATCCCGCCACTGGTGCAACTTCTTTCCTACCCAGATTCTAAAGTTCAACAGCATGCAGTAACGGCTCTTTTGAACTTATCGATTGATGAAACAAACAAGAAATTGATAACCAGAGAAGAAGCCATTCCAGCTATTATAGAAGTTTTGCAGAATGGAAGCACAGAAGCTAGAGAGAACTCTGCAGCAGCTTTATTTAGCTTGTCGATGATCGATGAGAACAAAGTAATTGTGGGGGAATCAAATGGCATTCCGCCATTAGTAGACTTGTTGCAGGACGGGACGATTAGAGGTAGAAAAGACGCTGCCACGGCACTGTTTAACTTATCTCTGAACCAAGCAAACAAGGGGAGGGCCATTAGTGCTGGCATTGTACCGCCACTGCTCCAGCTACTGAAGGACAGAAACTTGGGCATGGTCGATGAAGCGCTCTCCATCTTCTTACTTCTTGCATCACATCCAGATGGACGACAAGAGATTGGACAGCTCTCATTCATTGAAACTCTGGTGGAATTTATCCGGGAAGGAACACCAAAGAACAAGGAATGTGCAACGTCAGTTCTTCTCGAGTTGGGATCAAGCAATTCCTCTTTCTTACTTGCCGCACTGCAGTTTGGAGTGTATGAGCATTTGGTAGAGATCACAAGGAGTGGAACCAACAGAGCTCAAAGGAAGGCAAATGCACTTCTGCAACTCATAAGCAAGAGTGAACAAATTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

643

Amino Acids

71.29

Weight (kDa)

5.65

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB10_N PF25368 49 - 158 2.4e-34 PUB10-like, N-terminal domain
U-box PF04564 268 - 339 1.5e-24 U-box domain
ARM_PUB PF25598 361 - 637 6.4e-24 Plant U-box protein ARM repeat region
Arm PF00514 391 - 430 1.3e-11 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 392 - 584 9.2e-15 ARMC5
HEAT_2 PF13646 404 - 507 3.1e-06 HEAT repeats
Arm PF00514 474 - 512 5.8e-08 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 761
Acc36I ACCTGC 2 cut(s) 430, 603
AccI GTMKAC 1 cut(s) 1470
AccIII TCCGGA 1 cut(s) 229
AclWI GGATC 4 cut(s) 796, 842, 855, 1789
AcsI RAATTY 7 cut(s) 256, 318, 713, 1016, 1185, 1718, 1920
AcuI CTGAAG 1 cut(s) 1622
AfaI GTAC 1 cut(s) 1581
AfeI AGCGCT 1 cut(s) 1635
AfiI CCNNNNNNNGG 4 cut(s) 236, 918, 936, 1219
AflII CTTAAG 1 cut(s) 614
AflIII ACRYGT 1 cut(s) 923
AgsI TTSAA 9 cut(s) 107, 200, 262, 295, 335, 446, 1256, 1282, 1706
AjnI CCWGG 4 cut(s) 162, 670, 935, 1050
AjuI GAANNNNNNNTTGG 2 cut(s) 1172, 1204
Alw21I GWGCWC 3 cut(s) 216, 507, 1873
Alw26I GTCTC 4 cut(s) 18, 197, 617, 674
AlwI GGATC 4 cut(s) 796, 842, 855, 1789
AlwNI CAGNNNCTG 3 cut(s) 946, 1027, 1600
Ama87I CYCGRG 2 cut(s) 207, 1772
Aor13HI TCCGGA 1 cut(s) 229
Aor51HI AGCGCT 1 cut(s) 1635
AoxI GGCC 4 cut(s) 221, 668, 1048, 1560
ApeKI GCWGC 6 cut(s) 187, 242, 688, 1391, 1394, 1511
ApoI RAATTY 7 cut(s) 256, 318, 713, 1016, 1185, 1718, 1920
Asp700I GAANNNNTTC 3 cut(s) 1016, 1332, 1920
AspLEI GCGC 1 cut(s) 1636
AspS9I GGNCC 5 cut(s) 221, 610, 668, 1048, 1560
AsuC2I CCSGG 1 cut(s) 1727
AsuHPI GGTGA 2 cut(s) 16, 44
AvaI CYCGRG 2 cut(s) 207, 1772
AvaII GGWCC 1 cut(s) 610
BaeGI GKGCMC 1 cut(s) 791
BamHI GGATCC 1 cut(s) 847
BanII GRGCYC 3 cut(s) 216, 507, 1873
Bbv12I GWGCWC 3 cut(s) 216, 507, 1873
BbvI GCAGC 6 cut(s) 174, 229, 700, 1403, 1406, 1498
BccI CCATC 4 cut(s) 5, 212, 1649, 1664
BceAI ACGGC 2 cut(s) 1287, 1533
BciT130I CCWGG 4 cut(s) 164, 672, 937, 1052
BclI TGATCA 1 cut(s) 453
BcnI CCSGG 1 cut(s) 1727
BcoDI GTCTC 4 cut(s) 18, 197, 617, 674
BfaI CTAG 3 cut(s) 590, 833, 1377
BfmI CTRYAG 2 cut(s) 1389, 1814
BfoI RGCGCY 1 cut(s) 1637
BfrI CTTAAG 1 cut(s) 614
BfuAI ACCTGC 2 cut(s) 430, 603
BglII AGATCT 1 cut(s) 1083
Bme1390I CCNGG 5 cut(s) 164, 672, 937, 1052, 1727
Bme18I GGWCC 1 cut(s) 610
BmeT110I CYCGRG 2 cut(s) 207, 1772
BmgT120I GGNCC 5 cut(s) 221, 610, 668, 1048, 1560
BmiI GGNNCC 2 cut(s) 849, 1861
BmrFI CCNGG 5 cut(s) 164, 672, 937, 1052, 1727
BmsI GCATC 1 cut(s) 1667
BoxI GACNNNNGTC 1 cut(s) 746
BplI GAGNNNNNCTC 2 cut(s) 1070, 1102
BpmI CTGGAG 1 cut(s) 1577
BpuEI CTTGAG 2 cut(s) 800, 1096
BpuMI CCSGG 1 cut(s) 1727
Bsa29I ATCGAT 3 cut(s) 9, 1289, 1419
BsaI GGTCTC 1 cut(s) 674
BsaJI CCNNGG 3 cut(s) 606, 1044, 1515
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 4 cut(s) 236, 918, 936, 1219
Bse1I ACTGG 1 cut(s) 1224
Bse3DI GCAATG 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 4 cut(s) 164, 672, 937, 1052
BseCI ATCGAT 3 cut(s) 9, 1289, 1419
BseDI CCNNGG 3 cut(s) 606, 1044, 1515
BseGI GGATG 1 cut(s) 1663
BseLI CCNNNNNNNGG 4 cut(s) 236, 918, 936, 1219
BseMI GCAATG 1 cut(s) 516
BseNI ACTGG 1 cut(s) 1224
BseRI GAGGAG 8 cut(s) 75, 78, 81, 84, 449, 678, 1051, 1094
BseSI GKGCMC 1 cut(s) 791
BseXI GCAGC 6 cut(s) 174, 229, 700, 1403, 1406, 1498
BsgI GTGCAG 1 cut(s) 696
BshFI GGCC 4 cut(s) 223, 670, 1050, 1562
BshVI ATCGAT 3 cut(s) 9, 1289, 1419
BsiHKAI GWGCWC 3 cut(s) 216, 507, 1873
BsiHKCI CYCGRG 2 cut(s) 207, 1772
BsiSI CCGG 2 cut(s) 230, 1726
BslFI GGGAC 2 cut(s) 912, 1501
BslI CCNNNNNNNGG 4 cut(s) 236, 918, 936, 1219
BsmAI GTCTC 4 cut(s) 18, 197, 617, 674
BsmBI CGTCTC 1 cut(s) 18
BsmFI GGGAC 2 cut(s) 912, 1501
BsmI GAATGC 3 cut(s) 158, 1141, 1455
BsnI GGCC 4 cut(s) 223, 670, 1050, 1562
Bso31I GGTCTC 1 cut(s) 674
BsoBI CYCGRG 2 cut(s) 207, 1772
Bsp1286I GDGCHC 4 cut(s) 216, 507, 791, 1873
Bsp13I TCCGGA 1 cut(s) 229
BspANI GGCC 4 cut(s) 223, 670, 1050, 1562
BspDI ATCGAT 3 cut(s) 9, 1289, 1419
BspEI TCCGGA 1 cut(s) 229
BspHI TCATGA 3 cut(s) 370, 808, 1143
BspLI GGNNCC 2 cut(s) 849, 1861
BspMAI CTGCAG 2 cut(s) 1393, 1818
BspMI ACCTGC 2 cut(s) 430, 603
BspPI GGATC 4 cut(s) 796, 842, 855, 1789
BspTI CTTAAG 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 516
BsrI ACTGG 1 cut(s) 1224
BssECI CCNNGG 3 cut(s) 606, 1044, 1515
Bst2UI CCWGG 4 cut(s) 164, 672, 937, 1052
Bst4CI ACNGT 1 cut(s) 1524
BstAFI CTTAAG 1 cut(s) 614
BstC8I GCNNGC 4 cut(s) 507, 579, 1263, 1573
BstDEI CTNAG 2 cut(s) 464, 1120
BstDSI CCRYGG 2 cut(s) 1044, 1515
BstF5I GGATG 1 cut(s) 1663
BstH2I RGCGCY 1 cut(s) 1637
BstHHI GCGC 1 cut(s) 1636
BstMAI GTCTC 4 cut(s) 18, 197, 617, 674
BstNI CCWGG 4 cut(s) 164, 672, 937, 1052
BstNSI RCATGY 2 cut(s) 927, 1265
BstPAI GACNNNNGTC 1 cut(s) 746
BstSCI CCNGG 5 cut(s) 162, 670, 935, 1050, 1725
BstSFI CTRYAG 2 cut(s) 1389, 1814
BstSLI GKGCMC 1 cut(s) 791
BstV1I GCAGC 6 cut(s) 174, 229, 700, 1403, 1406, 1498
BstX2I RGATCY 2 cut(s) 847, 1083
BstYI RGATCY 2 cut(s) 847, 1083
Bsu15I ATCGAT 3 cut(s) 9, 1289, 1419
BsuRI GGCC 4 cut(s) 223, 670, 1050, 1562
BsuTUI ATCGAT 3 cut(s) 9, 1289, 1419
BtgI CCRYGG 2 cut(s) 1044, 1515
BtsCI GGATG 1 cut(s) 1663
BtsI GCAGTG 3 cut(s) 1004, 1586, 1811
BtsIMutI CAGTG 5 cut(s) 1004, 1217, 1520, 1586, 1811
BveI ACCTGC 2 cut(s) 430, 603
Cac8I GCNNGC 4 cut(s) 507, 579, 1263, 1573
CaiI CAGNNNCTG 3 cut(s) 946, 1027, 1600
CciI TCATGA 3 cut(s) 370, 808, 1143
CfoI GCGC 1 cut(s) 1636
Cfr13I GGNCC 5 cut(s) 221, 610, 668, 1048, 1560
ClaI ATCGAT 3 cut(s) 9, 1289, 1419
CseI GACGC 1 cut(s) 1517
CsiI ACCWGGT 1 cut(s) 162
Csp6I GTAC 1 cut(s) 1580
CviQI GTAC 1 cut(s) 1580
DdeI CTNAG 2 cut(s) 464, 1120
DrdI GACNNNNNNGTC 1 cut(s) 761
DseDI GACNNNNNNGTC 1 cut(s) 761
EciI GGCGGA 3 cut(s) 63, 72, 1449
Ecl136II GAGCTC 3 cut(s) 214, 505, 1871
Eco24I GRGCYC 3 cut(s) 216, 507, 1873
Eco31I GGTCTC 1 cut(s) 674
Eco47I GGWCC 1 cut(s) 610
Eco47III AGCGCT 1 cut(s) 1635
Eco53kI GAGCTC 3 cut(s) 214, 505, 1871
Eco57I CTGAAG 1 cut(s) 1622
Eco88I CYCGRG 2 cut(s) 207, 1772
EcoICRI GAGCTC 3 cut(s) 214, 505, 1871
EcoO109I RGGNCCY 1 cut(s) 610
EcoRII CCWGG 4 cut(s) 162, 670, 935, 1050
EcoT38I GRGCYC 3 cut(s) 216, 507, 1873
Esp3I CGTCTC 1 cut(s) 18
FaqI GGGAC 2 cut(s) 912, 1501
FauI CCCGC 1 cut(s) 1221
FbaI TGATCA 1 cut(s) 453
FblI GTMKAC 1 cut(s) 1470
FokI GGATG 1 cut(s) 1650
FriOI GRGCYC 3 cut(s) 216, 507, 1873
FspBI CTAG 3 cut(s) 590, 833, 1377
GlaI GCGC 1 cut(s) 1635
GsuI CTGGAG 1 cut(s) 1577
HaeII RGCGCY 1 cut(s) 1637
HaeIII GGCC 4 cut(s) 223, 670, 1050, 1562
HapII CCGG 2 cut(s) 230, 1726
HgaI GACGC 1 cut(s) 1517
HhaI GCGC 1 cut(s) 1636
Hin6I GCGC 1 cut(s) 1634
HinP1I GCGC 1 cut(s) 1634
HincII GTYRAC 1 cut(s) 1099
HindII GTYRAC 1 cut(s) 1099
HinfI GANTC 8 cut(s) 205, 378, 640, 943, 1150, 1209, 1244, 1445
HpaII CCGG 2 cut(s) 230, 1726
HphI GGTGA 2 cut(s) 16, 44
Hpy166II GTNNAC 4 cut(s) 869, 1099, 1471, 1916
Hpy188I TCNGA 7 cut(s) 23, 80, 125, 424, 607, 719, 1539
Hpy188III TCNNGA 9 cut(s) 200, 209, 230, 371, 809, 1027, 1144, 1667, 1772
Hpy8I GTNNAC 4 cut(s) 869, 1099, 1471, 1916
HpyAV CCTTC 8 cut(s) 23, 29, 89, 259, 389, 1597, 1724, 1873
HpyCH4III ACNGT 1 cut(s) 1524
HpyCH4IV ACGT 2 cut(s) 875, 1760
HpyF3I CTNAG 2 cut(s) 464, 1120
HpySE526I ACGT 2 cut(s) 875, 1760
HspAI GCGC 1 cut(s) 1634
Kpn2I TCCGGA 1 cut(s) 229
Ksp22I TGATCA 1 cut(s) 453
LmnI GCTCC 6 cut(s) 211, 312, 436, 1029, 1064, 1596
Lsp1109I GCAGC 6 cut(s) 174, 229, 700, 1403, 1406, 1498
LweI GCATC 1 cut(s) 1667
MabI ACCWGGT 1 cut(s) 162
MaeI CTAG 3 cut(s) 590, 833, 1377
MaeII ACGT 2 cut(s) 875, 1760
MaeIII GTNAC 3 cut(s) 957, 1115, 1267
MboII GAAGA 9 cut(s) 147, 274, 554, 695, 1021, 1159, 1337, 1636, 1760
MflI RGATCY 2 cut(s) 847, 1083
MhlI GDGCHC 4 cut(s) 216, 507, 791, 1873
MlyI GAGTC 3 cut(s) 199, 372, 937
MroI TCCGGA 1 cut(s) 229
MroXI GAANNNNTTC 3 cut(s) 1016, 1332, 1920
MseI TTAA 4 cut(s) 615, 648, 708, 1527
MslI CAYNNNNRTG 1 cut(s) 1668
MspA1I CMGCKG 1 cut(s) 1105
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 2 cut(s) 230, 1726
MspR9I CCNGG 5 cut(s) 164, 672, 937, 1052, 1727
Mva1269I GAATGC 3 cut(s) 158, 1141, 1455
MvaI CCWGG 4 cut(s) 164, 672, 937, 1052
NciI CCSGG 1 cut(s) 1727
NlaIV GGNNCC 2 cut(s) 849, 1861
NmuCI GTSAC 2 cut(s) 957, 1115
NspI RCATGY 2 cut(s) 927, 1265
PaeI GCATGC 1 cut(s) 1265
PaeR7I CTCGAG 1 cut(s) 1772
PagI TCATGA 3 cut(s) 370, 808, 1143
PciI ACATGT 1 cut(s) 923
PctI GAATGC 3 cut(s) 158, 1141, 1455
PdmI GAANNNNTTC 3 cut(s) 1016, 1332, 1920
PfeI GAWTC 5 cut(s) 640, 1150, 1209, 1244, 1445
PflFI GACNNNGTC 1 cut(s) 381
PfoI TCCNGGA 1 cut(s) 1725
PleI GAGTC 3 cut(s) 199, 372, 937
PpsI GAGTC 3 cut(s) 199, 372, 937
PpuMI RGGWCCY 1 cut(s) 610
PscI ACATGT 1 cut(s) 923
PshAI GACNNNNGTC 1 cut(s) 746
Psp124BI GAGCTC 3 cut(s) 216, 507, 1873
Psp5II RGGWCCY 1 cut(s) 610
Psp6I CCWGG 4 cut(s) 162, 670, 935, 1050
PspGI CCWGG 4 cut(s) 162, 670, 935, 1050
PspN4I GGNNCC 2 cut(s) 849, 1861
PspPI GGNCC 5 cut(s) 221, 610, 668, 1048, 1560
PspPPI RGGWCCY 1 cut(s) 610
PstI CTGCAG 2 cut(s) 1393, 1818
PstNI CAGNNNCTG 3 cut(s) 946, 1027, 1600
PsuI RGATCY 2 cut(s) 847, 1083
PsyI GACNNNGTC 1 cut(s) 381
PvuII CAGCTG 1 cut(s) 1105
RsaI GTAC 1 cut(s) 1581
RsaNI GTAC 1 cut(s) 1580
RseI CAYNNNNRTG 1 cut(s) 1668
SacI GAGCTC 3 cut(s) 216, 507, 1873
SaqAI TTAA 4 cut(s) 615, 648, 708, 1527
Sau96I GGNCC 5 cut(s) 221, 610, 668, 1048, 1560
SchI GAGTC 3 cut(s) 199, 372, 937
ScrFI CCNGG 5 cut(s) 164, 672, 937, 1052, 1727
SduI GDGCHC 4 cut(s) 216, 507, 791, 1873
SexAI ACCWGGT 1 cut(s) 162
SfaNI GCATC 1 cut(s) 1667
SfcI CTRYAG 2 cut(s) 1389, 1814
Sfr274I CTCGAG 1 cut(s) 1772
SinI GGWCC 1 cut(s) 610
SlaI CTCGAG 1 cut(s) 1772
SmiMI CAYNNNNRTG 1 cut(s) 1668
SmlI CTYRAG 4 cut(s) 614, 779, 1111, 1772
SmoI CTYRAG 4 cut(s) 614, 779, 1111, 1772
SphI GCATGC 1 cut(s) 1265
SspMI CTAG 3 cut(s) 590, 833, 1377
SstI GAGCTC 3 cut(s) 216, 507, 1873
StyD4I CCNGG 5 cut(s) 162, 670, 935, 1050, 1725
TaaI ACNGT 1 cut(s) 1524
TaiI ACGT 2 cut(s) 878, 1763
TaqI TCGA 8 cut(s) 9, 216, 906, 1289, 1412, 1419, 1626, 1773
TaqII GACCGA 1 cut(s) 371
TauI GCSGC 4 cut(s) 57, 172, 193, 1811
TfiI GAWTC 5 cut(s) 640, 1150, 1209, 1244, 1445
Tru1I TTAA 4 cut(s) 615, 648, 708, 1527
Tru9I TTAA 4 cut(s) 615, 648, 708, 1527
TscAI CASTG 5 cut(s) 1004, 1224, 1527, 1593, 1818
TseFI GTSAC 2 cut(s) 957, 1115
TseI GCWGC 6 cut(s) 187, 242, 688, 1391, 1394, 1511
Tsp45I GTSAC 2 cut(s) 957, 1115
TspGWI ACGGA 1 cut(s) 860
TspRI CASTG 5 cut(s) 1004, 1224, 1527, 1593, 1818
Tth111I GACNNNGTC 1 cut(s) 381
Vha464I CTTAAG 1 cut(s) 614
VpaK11BI GGWCC 1 cut(s) 610
XapI RAATTY 7 cut(s) 256, 318, 713, 1016, 1185, 1718, 1920
XceI RCATGY 2 cut(s) 927, 1265
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XhoI CTCGAG 1 cut(s) 1772
XmiI GTMKAC 1 cut(s) 1470
XmnI GAANNNNTTC 3 cut(s) 1016, 1332, 1920
XspI CTAG 3 cut(s) 590, 833, 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.