pycom15g01600

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012802
Physical Location & Seq
Reverse (-)
926851 .. 928459
1609 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1074 bp
ATGGCTGGCTTTTTTTGCTTAGGAGGGAGAGATCGAAGTCCGAACAGCCGCGGCAAACAAGAGGGAGAGAATCAGAGCGGAAGAGTGGGAGGAGTAGCAGCGGAAGCGGCGGCAGGAGGAGGAGGAGGGAACTTGTTTTTGTACAGATCAACAGGCGAGGCGCAGGCCGAGATCTACAACAAGGGGTTTGAGATATGGCCGTCACAATACCATCCACACCAAAACTTGAACTACTACTCGTTTGGAGTGGATCCTAGCTACAACCGTAGACACTTGGACAACAATAACGACGGCGTTTCGGCGGATGATCTATCGGCAGGGTTGAGGCTGACGGTGATGAGGGGAGGAGGCTTAGGAAGCAGCGGCGGTGGCATAAACTGTCAAGACTGCGGGAACCAAGCGAAGAAAGACTGTCCGCACCTTAGGTGCCGGACCTGTTGCAAGAGCCGAGGGTTTCAGTGTCAAACACACGTGAAGAGCACGTGGGTTCCCGCCGCGAAACGACGGGAGCGGCAGGAACAATTCTCGGCCTTACAACAAAACCGAAATCAGCAACAACAGCAGATACAGTTTCGAGGTGAAAACCCCAAAAGGCAGAGAGATAACTCTCTTGCCTGCGTTCGTATTCCTTCCGACACGTCAGGGTTGGAGTTGAATCCATTTCCGCCAGAAGTGAACTCACCGGCGGTGTTCCGCTGCGTAAAAGTAAGTGCAATGGACGATGTAGATGAGCAATTCGCGTATCAAACGGCTGTCAACATCGGAGGGCATGTGTTCAAGGGACTTCTGTATGATCAAGGCATCGATGGTCAGTATTACCCGAGTTCTGGAGGAGGAGGTGAGAGCTCCTCGGGAGGTCAAGGTACCAGTCACGATCATCACCAAGACGGAGGAGGAAGCGGAGGAGGAGGAGGTCATCCACATCATCATCAGCAACATAATCTTGTAACGGTCGCTGCTGCCGGCGGGACCTCTGGCAATCCGTCTACTACGTTGCTTGACCCTTCTCTCTTCCCAGCTCCACTCAATGCTTTCATGGCTGGTACGCAATACTTTCCGCCTCCCAGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

358

Amino Acids

38.18

Weight (kDa)

7.63

Isoelectric Point (pI)

51.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 124 - 269 1.4e-64 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19790 AT1G19790 AT1G75520
fragaria_vesca FvH4_2g38950
malus_domestica MD08G1021500.v1.1 MD15G1019200.v1.1
prunus_persica Prupe.1G371500_v2.0.a1 Prupe.1G371500_v2.0.a1
pyrus_communis pycom08g01700 pycom15g01600
rosa_chinensis RchiOBHm_Chr6g0302041
rosa_laevigata RLG00000011200
rosa_multiflora Rmu_ssc0000160.1_g000001
rosa_roxburghii Rroxscaffold_7G00166150
rosa_rugosa Rorug06G0311300
rosa_samantha Rh6AG424100 Rh6CG437100
rosa_wichuraiana Rw6G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 2 cut(s) 426, 863
AccBSI CCGCTC 2 cut(s) 78, 511
AccI GTMKAC 2 cut(s) 268, 986
AccII CGCG 3 cut(s) 51, 497, 740
AclWI GGATC 2 cut(s) 245, 258
AcoI YGGCCR 1 cut(s) 197
AcvI CACGTG 2 cut(s) 472, 483
AfaI GTAC 3 cut(s) 143, 865, 1045
AfiI CCNNNNNNNGG 1 cut(s) 827
AflIII ACRYGT 2 cut(s) 469, 636
AgsI TTSAA 3 cut(s) 229, 655, 778
AjiI CACGTC 1 cut(s) 639
AjnI CCWGG 1 cut(s) 1064
AluBI AGCT 3 cut(s) 258, 846, 1019
AluI AGCT 3 cut(s) 258, 846, 1019
Alw21I GWGCWC 2 cut(s) 482, 848
AlwI GGATC 2 cut(s) 245, 258
Ama87I CYCGRG 2 cut(s) 820, 850
AoxI GGCC 3 cut(s) 165, 197, 528
ApeKI GCWGC 5 cut(s) 98, 360, 696, 956, 959
Asp718I GGTACC 1 cut(s) 863
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 2 cut(s) 432, 969
AsuHPI GGTGA 5 cut(s) 346, 590, 672, 851, 872
AvaI CYCGRG 2 cut(s) 820, 850
AvaII GGWCC 2 cut(s) 432, 969
AxyI CCTNAGG 1 cut(s) 422
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 2 cut(s) 426, 863
BanII GRGCYC 1 cut(s) 848
BbrPI CACGTG 2 cut(s) 472, 483
Bbv12I GWGCWC 2 cut(s) 482, 848
BbvI GCAGC 5 cut(s) 110, 372, 683, 943, 946
BccI CCATC 2 cut(s) 219, 800
BceAI ACGGC 3 cut(s) 184, 307, 765
BciT130I CCWGG 1 cut(s) 1066
BclI TGATCA 1 cut(s) 793
BfaI CTAG 1 cut(s) 255
BglII AGATCT 1 cut(s) 171
Bme1390I CCNGG 1 cut(s) 1066
Bme18I GGWCC 2 cut(s) 432, 969
BmeT110I CYCGRG 2 cut(s) 820, 850
BmgBI CACGTC 1 cut(s) 639
BmgT120I GGNCC 2 cut(s) 432, 969
BmiI GGNNCC 6 cut(s) 252, 395, 428, 489, 865, 970
BmrFI CCNGG 1 cut(s) 1066
BmsI GCATC 1 cut(s) 810
BplI GAGNNNNNCTC 2 cut(s) 833, 865
BpmI CTGGAG 1 cut(s) 849
Bpu10I CCTNAGC 2 cut(s) 19, 352
Bsa29I ATCGAT 1 cut(s) 804
BsaAI YACGTR 2 cut(s) 472, 483
BsaJI CCNNGG 4 cut(s) 49, 448, 849, 1064
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 1 cut(s) 827
Bse118I RCCGGY 2 cut(s) 682, 962
Bse1I ACTGG 1 cut(s) 867
Bse21I CCTNAGG 1 cut(s) 422
Bse3DI GCAATG 1 cut(s) 720
BseBI CCWGG 1 cut(s) 1066
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 4 cut(s) 49, 448, 849, 1064
BseGI GGATG 3 cut(s) 211, 310, 916
BseLI CCNNNNNNNGG 1 cut(s) 827
BseMI GCAATG 1 cut(s) 720
BseNI ACTGG 1 cut(s) 867
BseXI GCAGC 5 cut(s) 110, 372, 683, 943, 946
BseYI CCCAGC 1 cut(s) 1015
Bsh1236I CGCG 3 cut(s) 51, 497, 740
Bsh1285I CGRYCG 1 cut(s) 954
BshFI GGCC 3 cut(s) 167, 199, 530
BshNI GGYRCC 2 cut(s) 426, 863
BshVI ATCGAT 1 cut(s) 804
BsiEI CGRYCG 1 cut(s) 954
BsiHKAI GWGCWC 2 cut(s) 482, 848
BsiHKCI CYCGRG 2 cut(s) 820, 850
BsiSI CCGG 3 cut(s) 430, 683, 963
BslFI GGGAC 2 cut(s) 795, 982
BslI CCNNNNNNNGG 1 cut(s) 827
BsmFI GGGAC 2 cut(s) 795, 982
BsnI GGCC 3 cut(s) 167, 199, 530
BsoBI CYCGRG 2 cut(s) 820, 850
Bsp1286I GDGCHC 2 cut(s) 482, 848
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 7 cut(s) 31, 146, 171, 250, 307, 793, 874
BspANI GGCC 3 cut(s) 167, 199, 530
BspDI ATCGAT 1 cut(s) 804
BspFNI CGCG 3 cut(s) 51, 497, 740
BspLI GGNNCC 6 cut(s) 252, 395, 428, 489, 865, 970
BspPI GGATC 2 cut(s) 245, 258
BspQI GCTCTTC 1 cut(s) 470
BspT107I GGYRCC 2 cut(s) 426, 863
BsrBI CCGCTC 2 cut(s) 78, 511
BsrDI GCAATG 1 cut(s) 720
BsrFI RCCGGY 2 cut(s) 682, 962
BsrGI TGTACA 1 cut(s) 141
BsrI ACTGG 1 cut(s) 867
BssAI RCCGGY 2 cut(s) 682, 962
BssECI CCNNGG 4 cut(s) 49, 448, 849, 1064
BssMI GATC 7 cut(s) 31, 146, 171, 250, 307, 793, 874
Bst2UI CCWGG 1 cut(s) 1066
Bst4CI ACNGT 6 cut(s) 266, 334, 380, 413, 570, 952
Bst6I CTCTTC 3 cut(s) 76, 470, 1016
BstAUI TGTACA 1 cut(s) 141
BstBAI YACGTR 2 cut(s) 472, 483
BstC8I GCNNGC 4 cut(s) 7, 165, 616, 964
BstDEI CTNAG 3 cut(s) 19, 352, 422
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 3 cut(s) 211, 310, 916
BstFNI CGCG 3 cut(s) 51, 497, 740
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 7 cut(s) 34, 149, 174, 253, 310, 796, 877
BstMBI GATC 7 cut(s) 31, 146, 171, 250, 307, 793, 874
BstMCI CGRYCG 1 cut(s) 954
BstMWI GCNNNNNNNGC 7 cut(s) 15, 104, 107, 357, 369, 559, 1037
BstNI CCWGG 1 cut(s) 1066
BstNSI RCATGY 1 cut(s) 773
BstSCI CCNGG 1 cut(s) 1064
BstUI CGCG 3 cut(s) 51, 497, 740
BstV1I GCAGC 5 cut(s) 110, 372, 683, 943, 946
BstX2I RGATCY 2 cut(s) 171, 250
BstYI RGATCY 2 cut(s) 171, 250
Bsu15I ATCGAT 1 cut(s) 804
Bsu36I CCTNAGG 1 cut(s) 422
BsuRI GGCC 3 cut(s) 167, 199, 530
BsuTUI ATCGAT 1 cut(s) 804
BtgI CCRYGG 1 cut(s) 49
BtrI CACGTC 1 cut(s) 639
BtsCI GGATG 3 cut(s) 211, 310, 916
BtsIMutI CAGTG 1 cut(s) 464
Cac8I GCNNGC 4 cut(s) 7, 165, 616, 964
CfoI GCGC 1 cut(s) 163
Cfr10I RCCGGY 2 cut(s) 682, 962
Cfr13I GGNCC 2 cut(s) 432, 969
Cfr42I CCGCGG 1 cut(s) 52
ClaI ATCGAT 1 cut(s) 804
Csp6I GTAC 3 cut(s) 142, 864, 1044
CviAII CATG 2 cut(s) 770, 1036
CviQI GTAC 3 cut(s) 142, 864, 1044
DdeI CTNAG 3 cut(s) 19, 352, 422
DpnI GATC 7 cut(s) 33, 148, 173, 252, 309, 795, 876
DpnII GATC 7 cut(s) 31, 146, 171, 250, 307, 793, 874
EaeI YGGCCR 1 cut(s) 197
Eam1104I CTCTTC 3 cut(s) 76, 470, 1016
EarI CTCTTC 3 cut(s) 76, 470, 1016
EciI GGCGGA 3 cut(s) 317, 654, 1047
Ecl136II GAGCTC 1 cut(s) 846
Eco24I GRGCYC 1 cut(s) 848
Eco47I GGWCC 2 cut(s) 432, 969
Eco53kI GAGCTC 1 cut(s) 846
Eco72I CACGTG 2 cut(s) 472, 483
Eco81I CCTNAGG 1 cut(s) 422
Eco88I CYCGRG 2 cut(s) 820, 850
EcoICRI GAGCTC 1 cut(s) 846
EcoO109I RGGNCCY 1 cut(s) 969
EcoRII CCWGG 1 cut(s) 1064
EcoT38I GRGCYC 1 cut(s) 848
FaeI CATG 2 cut(s) 773, 1039
FaiI YATR 6 cut(s) 196, 374, 771, 792, 939, 1037
FaqI GGGAC 2 cut(s) 795, 982
FatI CATG 2 cut(s) 769, 1035
FauI CCCGC 3 cut(s) 383, 499, 959
FbaI TGATCA 1 cut(s) 793
FblI GTMKAC 2 cut(s) 268, 986
FokI GGATG 3 cut(s) 198, 317, 903
FriOI GRGCYC 1 cut(s) 848
FspBI CTAG 1 cut(s) 255
GlaI GCGC 1 cut(s) 162
GsaI CCCAGC 1 cut(s) 1019
GsuI CTGGAG 1 cut(s) 849
HaeIII GGCC 3 cut(s) 167, 199, 530
HapII CCGG 3 cut(s) 430, 683, 963
HhaI GCGC 1 cut(s) 163
Hin1II CATG 2 cut(s) 773, 1039
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HinfI GANTC 2 cut(s) 70, 655
HpaII CCGG 3 cut(s) 430, 683, 963
HphI GGTGA 5 cut(s) 346, 590, 672, 851, 872
Hpy166II GTNNAC 4 cut(s) 269, 676, 757, 987
Hpy188I TCNGA 4 cut(s) 42, 75, 634, 764
Hpy188III TCNNGA 4 cut(s) 383, 828, 852, 872
Hpy8I GTNNAC 4 cut(s) 269, 676, 757, 987
Hpy99I CGWCG 2 cut(s) 293, 507
HpyAV CCTTC 2 cut(s) 639, 1014
HpyCH4III ACNGT 6 cut(s) 266, 334, 380, 413, 570, 952
HpyCH4IV ACGT 4 cut(s) 471, 482, 638, 992
HpyCH4V TGCA 2 cut(s) 441, 713
HpyF10VI GCNNNNNNNGC 7 cut(s) 15, 104, 107, 357, 369, 559, 1037
HpyF3I CTNAG 3 cut(s) 19, 352, 422
HpySE526I ACGT 4 cut(s) 471, 482, 638, 992
Hsp92II CATG 2 cut(s) 773, 1039
HspAI GCGC 1 cut(s) 161
KpnI GGTACC 1 cut(s) 867
KroI GCCGGC 1 cut(s) 962
KroNI GCCGGC 1 cut(s) 964
Ksp22I TGATCA 1 cut(s) 793
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 7 cut(s) 31, 146, 171, 250, 307, 793, 874
LguI GCTCTTC 1 cut(s) 470
LmnI GCTCC 3 cut(s) 508, 851, 1024
Lsp1109I GCAGC 5 cut(s) 110, 372, 683, 943, 946
LweI GCATC 1 cut(s) 810
MaeI CTAG 1 cut(s) 255
MaeII ACGT 4 cut(s) 471, 482, 638, 992
MaeIII GTNAC 3 cut(s) 201, 869, 946
MalI GATC 7 cut(s) 33, 148, 173, 252, 309, 795, 876
MbiI CCGCTC 2 cut(s) 78, 511
MboI GATC 7 cut(s) 31, 146, 171, 250, 307, 793, 874
MboII GAAGA 4 cut(s) 93, 415, 487, 1003
MflI RGATCY 2 cut(s) 171, 250
MhlI GDGCHC 2 cut(s) 482, 848
MluCI AATT 2 cut(s) 521, 734
MmeI TCCRAC 2 cut(s) 627, 657
MroNI GCCGGC 1 cut(s) 962
MseI TTAA 1 cut(s) 1072
MspA1I CMGCKG 4 cut(s) 51, 101, 363, 696
MspI CCGG 3 cut(s) 430, 683, 963
MspR9I CCNGG 1 cut(s) 1066
MvaI CCWGG 1 cut(s) 1066
MvnI CGCG 3 cut(s) 51, 497, 740
MwoI GCNNNNNNNGC 7 cut(s) 15, 104, 107, 357, 369, 559, 1037
NaeI GCCGGC 1 cut(s) 964
NdeII GATC 7 cut(s) 31, 146, 171, 250, 307, 793, 874
NgoMIV GCCGGC 1 cut(s) 962
NlaIII CATG 2 cut(s) 773, 1039
NlaIV GGNNCC 6 cut(s) 252, 395, 428, 489, 865, 970
NmeAIII GCCGAG 3 cut(s) 193, 473, 506
NmuCI GTSAC 2 cut(s) 201, 869
NspI RCATGY 1 cut(s) 773
PciSI GCTCTTC 1 cut(s) 470
PdiI GCCGGC 1 cut(s) 964
PfeI GAWTC 2 cut(s) 70, 655
PmaCI CACGTG 2 cut(s) 472, 483
PmlI CACGTG 2 cut(s) 472, 483
Ppu21I YACGTR 2 cut(s) 472, 483
PpuMI RGGWCCY 1 cut(s) 969
Psp124BI GAGCTC 1 cut(s) 848
Psp5II RGGWCCY 1 cut(s) 969
Psp6I CCWGG 1 cut(s) 1064
PspCI CACGTG 2 cut(s) 472, 483
PspFI CCCAGC 1 cut(s) 1015
PspGI CCWGG 1 cut(s) 1064
PspN4I GGNNCC 6 cut(s) 252, 395, 428, 489, 865, 970
PspPI GGNCC 2 cut(s) 432, 969
PspPPI RGGWCCY 1 cut(s) 969
PsuI RGATCY 2 cut(s) 171, 250
RsaI GTAC 3 cut(s) 143, 865, 1045
RsaNI GTAC 3 cut(s) 142, 864, 1044
SacI GAGCTC 1 cut(s) 848
SacII CCGCGG 1 cut(s) 52
SapI GCTCTTC 1 cut(s) 470
SaqAI TTAA 1 cut(s) 1072
Sau3AI GATC 7 cut(s) 31, 146, 171, 250, 307, 793, 874
Sau96I GGNCC 2 cut(s) 432, 969
ScrFI CCNGG 1 cut(s) 1066
SduI GDGCHC 2 cut(s) 482, 848
SfaNI GCATC 1 cut(s) 810
Sfr303I CCGCGG 1 cut(s) 52
SgrAI CRCCGGYG 1 cut(s) 682
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 2 cut(s) 432, 969
Sse9I AATT 2 cut(s) 521, 734
SspMI CTAG 1 cut(s) 255
SstI GAGCTC 1 cut(s) 848
StyD4I CCNGG 1 cut(s) 1064
TaaI ACNGT 6 cut(s) 266, 334, 380, 413, 570, 952
TaiI ACGT 4 cut(s) 474, 485, 641, 995
TaqI TCGA 3 cut(s) 34, 574, 804
TasI AATT 2 cut(s) 521, 734
TatI WGTACW 1 cut(s) 141
TauI GCSGC 7 cut(s) 51, 54, 110, 113, 366, 497, 514
TfiI GAWTC 2 cut(s) 70, 655
Tru1I TTAA 1 cut(s) 1072
Tru9I TTAA 1 cut(s) 1072
TscAI CASTG 1 cut(s) 464
TseFI GTSAC 2 cut(s) 201, 869
TseI GCWGC 5 cut(s) 98, 360, 696, 956, 959
Tsp45I GTSAC 2 cut(s) 201, 869
TspDTI ATGAA 1 cut(s) 1024
TspGWI ACGGA 2 cut(s) 903, 972
TspRI CASTG 1 cut(s) 464
VpaK11BI GGWCC 2 cut(s) 432, 969
XceI RCATGY 1 cut(s) 773
XmiI GTMKAC 2 cut(s) 268, 986
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.