pycom15g02700

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014678
Physical Location & Seq
Forward (+)
1649572 .. 1651034
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGAAGGACAACGATTCACCGACACTGATAACAATCCCATCATCAACGGTGATCAGGAAGGACACCTCGTCGTCGAAAGCCAGAGGCTTTCTCGACAAGAATCGCTACAAGTTCTCGGTTTTAGCTGCGATTCTCCTCCTCGCTTTCTGGTCAATGTTCACGGGGTGCGTCACCCTCAAATGGTCCGCCGGCAACTTATCCCACCTCTCCGACGACCTCGATATCCCGACCTTCGACGATCTCGACATTCTAGAGGTGGAGGAGAGAGAGAAGGTAGTGAGGCACATGTGGGATTTGTACACGCAGAGCAGCAGCAGCAGCAGCACGAGTCGACTGCCGCTGTTTTGGCAGGAGGCTTTCGAGGCGGCGTACGAGCATTTGAGCAGCGACGTTGCTGCCGTTCGAGACGCCGCCGTTTCGGAGATTGCCAAGATGTCCATCCGCTCCATCGTTCTTGATCCCATCCCACTTCAATCAACGAACATAAGAGAAATGCAGAAGAAGAGATCCAAGAAAGCGGAGGAAGGCAAGAAGGTGACTACATTACGAAGTAGTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

187

Amino Acids

20.91

Weight (kDa)

6.84

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 13 - 155 5.1e-62 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 444
AccI GTMKAC 1 cut(s) 331
AciI CCGC 6 cut(s) 186, 338, 365, 411, 442, 518
AclWI GGATC 2 cut(s) 452, 501
AcyI GRCGYC 1 cut(s) 408
AdeI CACNNNGTG 1 cut(s) 165
AfaI GTAC 2 cut(s) 299, 371
AfiI CCNNNNNNNGG 1 cut(s) 180
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 1 cut(s) 473
AhdI GACNNNNNGTC 1 cut(s) 67
AluBI AGCT 2 cut(s) 125, 558
AluI AGCT 2 cut(s) 125, 558
Alw26I GTCTC 1 cut(s) 399
AlwI GGATC 2 cut(s) 452, 501
ApeKI GCWGC 8 cut(s) 125, 309, 312, 315, 318, 321, 384, 395
AspS9I GGNCC 1 cut(s) 183
AsuHPI GGTGA 4 cut(s) 9, 61, 163, 547
AvaII GGWCC 1 cut(s) 183
BauI CACGAG 1 cut(s) 325
BbvI GCAGC 8 cut(s) 112, 321, 324, 327, 330, 333, 382, 396
BccI CCATC 4 cut(s) 46, 446, 455, 470
BceAI ACGGC 2 cut(s) 383, 398
BcgI CGANNNNNNTGC 4 cut(s) 316, 350, 377, 411
BclI TGATCA 1 cut(s) 51
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 2 cut(s) 251, 559
Bme18I GGWCC 1 cut(s) 183
BmeRI GACNNNNNGTC 1 cut(s) 67
BmgT120I GGNCC 1 cut(s) 183
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BsaBI GATNNNNATC 2 cut(s) 32, 437
BsaHI GRCGYC 1 cut(s) 408
Bsc4I CCNNNNNNNGG 1 cut(s) 180
Bse118I RCCGGY 1 cut(s) 188
Bse8I GATNNNNATC 2 cut(s) 32, 437
BseGI GGATG 2 cut(s) 438, 462
BseJI GATNNNNATC 2 cut(s) 32, 437
BseLI CCNNNNNNNGG 1 cut(s) 180
BseRI GAGGAG 3 cut(s) 125, 128, 275
BseXI GCAGC 8 cut(s) 112, 321, 324, 327, 330, 333, 382, 396
BsiSI CCGG 1 cut(s) 189
BsiWI CGTACG 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 180
BsmAI GTCTC 1 cut(s) 399
BsmBI CGTCTC 1 cut(s) 399
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 4 cut(s) 51, 238, 457, 506
BspACI CCGC 6 cut(s) 186, 338, 365, 411, 442, 518
BspPI GGATC 2 cut(s) 452, 501
BsrBI CCGCTC 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 188
BsrGI TGTACA 1 cut(s) 297
BssAI RCCGGY 1 cut(s) 188
BssMI GATC 4 cut(s) 51, 238, 457, 506
BssNI GRCGYC 1 cut(s) 408
BssSI CACGAG 1 cut(s) 325
Bst2BI CACGAG 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 1 cut(s) 497
BstACI GRCGYC 1 cut(s) 408
BstAUI TGTACA 1 cut(s) 297
BstC8I GCNNGC 1 cut(s) 190
BstF5I GGATG 2 cut(s) 438, 462
BstKTI GATC 4 cut(s) 54, 241, 460, 509
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 4 cut(s) 51, 238, 457, 506
BstMWI GCNNNNNNNGC 5 cut(s) 315, 318, 321, 346, 362
BstNSI RCATGY 1 cut(s) 289
BstV1I GCAGC 8 cut(s) 112, 321, 324, 327, 330, 333, 382, 396
BstX2I RGATCY 1 cut(s) 506
BstYI RGATCY 1 cut(s) 506
BtsCI GGATG 2 cut(s) 438, 462
BtsIMutI CAGTG 1 cut(s) 23
Cac8I GCNNGC 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 183
CseI GACGC 2 cut(s) 157, 416
Csp6I GTAC 2 cut(s) 298, 370
CviAII CATG 1 cut(s) 286
CviJI RGCY 5 cut(s) 80, 87, 125, 356, 558
CviKI_1 RGCY 5 cut(s) 80, 87, 125, 356, 558
CviQI GTAC 2 cut(s) 298, 370
DpnI GATC 4 cut(s) 53, 240, 459, 508
DpnII GATC 4 cut(s) 51, 238, 457, 506
DraIII CACNNNGTG 1 cut(s) 165
DriI GACNNNNNGTC 1 cut(s) 67
Eam1104I CTCTTC 1 cut(s) 497
Eam1105I GACNNNNNGTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 497
EciI GGCGGA 1 cut(s) 175
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 183
EcoRV GATATC 1 cut(s) 223
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 1 cut(s) 289
FaiI YATR 2 cut(s) 287, 485
FatI CATG 1 cut(s) 285
FbaI TGATCA 1 cut(s) 51
FblI GTMKAC 1 cut(s) 331
FokI GGATG 2 cut(s) 425, 449
FspBI CTAG 2 cut(s) 251, 559
HapII CCGG 1 cut(s) 189
HgaI GACGC 2 cut(s) 157, 416
Hin1I GRCGYC 1 cut(s) 408
Hin1II CATG 1 cut(s) 289
HincII GTYRAC 1 cut(s) 332
HindII GTYRAC 1 cut(s) 332
HinfI GANTC 4 cut(s) 14, 100, 130, 328
HpaII CCGG 1 cut(s) 189
HphI GGTGA 4 cut(s) 9, 61, 163, 547
Hpy166II GTNNAC 3 cut(s) 159, 300, 332
Hpy188I TCNGA 2 cut(s) 211, 421
Hpy188III TCNNGA 7 cut(s) 55, 92, 226, 242, 251, 404, 455
Hpy8I GTNNAC 3 cut(s) 159, 300, 332
Hpy99I CGWCG 5 cut(s) 73, 76, 215, 239, 392
HpyAV CCTTC 5 cut(s) 52, 241, 265, 518, 526
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 1 cut(s) 496
HpyF10VI GCNNNNNNNGC 5 cut(s) 315, 318, 321, 346, 362
HpySE526I ACGT 1 cut(s) 390
Hsp92I GRCGYC 1 cut(s) 408
Hsp92II CATG 1 cut(s) 289
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 51
Kzo9I GATC 4 cut(s) 51, 238, 457, 506
LmnI GCTCC 1 cut(s) 449
LpnPI CCDG 5 cut(s) 40, 94, 133, 202, 335
Lsp1109I GCAGC 8 cut(s) 112, 321, 324, 327, 330, 333, 382, 396
MaeI CTAG 2 cut(s) 251, 559
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 169, 535
MalI GATC 4 cut(s) 53, 240, 459, 508
MbiI CCGCTC 1 cut(s) 444
MboI GATC 4 cut(s) 51, 238, 457, 506
MboII GAAGA 2 cut(s) 511, 514
MflI RGATCY 1 cut(s) 506
MlyI GAGTC 1 cut(s) 337
MmeI TCCRAC 1 cut(s) 234
MroNI GCCGGC 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 340
MspI CCGG 1 cut(s) 189
MwoI GCNNNNNNNGC 5 cut(s) 315, 318, 321, 346, 362
NaeI GCCGGC 1 cut(s) 190
NdeII GATC 4 cut(s) 51, 238, 457, 506
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 1 cut(s) 289
NmuCI GTSAC 2 cut(s) 169, 535
NspI RCATGY 1 cut(s) 289
PciI ACATGT 1 cut(s) 285
PcsI WCGNNNNNNNCGW 2 cut(s) 240, 396
PdiI GCCGGC 1 cut(s) 190
PfeI GAWTC 3 cut(s) 14, 100, 130
Pfl23II CGTACG 1 cut(s) 369
PleI GAGTC 1 cut(s) 336
PpsI GAGTC 1 cut(s) 336
PscI ACATGT 1 cut(s) 285
PspLI CGTACG 1 cut(s) 369
PspPI GGNCC 1 cut(s) 183
PsuI RGATCY 1 cut(s) 506
RsaI GTAC 2 cut(s) 299, 371
RsaNI GTAC 2 cut(s) 298, 370
SalI GTCGAC 1 cut(s) 330
Sau3AI GATC 4 cut(s) 51, 238, 457, 506
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 1 cut(s) 337
SinI GGWCC 1 cut(s) 183
SsiI CCGC 6 cut(s) 186, 338, 365, 411, 442, 518
SspMI CTAG 2 cut(s) 251, 559
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 1 cut(s) 393
TaqI TCGA 8 cut(s) 74, 93, 219, 234, 243, 331, 360, 403
TatI WGTACW 1 cut(s) 297
TauI GCSGC 3 cut(s) 340, 368, 413
TfiI GAWTC 3 cut(s) 14, 100, 130
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 2 cut(s) 169, 535
TseI GCWGC 8 cut(s) 125, 309, 312, 315, 318, 321, 384, 395
Tsp45I GTSAC 2 cut(s) 169, 535
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 30
VpaK11BI GGWCC 1 cut(s) 183
XbaI TCTAGA 1 cut(s) 250
XceI RCATGY 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 331
XspI CTAG 2 cut(s) 251, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.