pycom15g05480

BAAT / Acyl-CoA thioester hydrolase C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009765
Physical Location & Seq
Forward (+)
3268489 .. 3270391
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
ATGTCGGTGACTCGGCCGACTCCGACTCAGAAGCAACCAGTTACTCAACAGCGCAGGATTGTTGTAGAAAACAAGCATGGGGAGAAGCTTGTCGGGATATTACATGAGAGGGGTTCGAAGGATCTCGTTGTGGTGTGCCATGGCATCCACTCATCAAAGGAACGCATTCCCATGGTGAACATTGCCGCTGTGTTAGAAAACGATGGTATAAGCGCCTTCCGCTTTGACTTCGCTGGAAACGGGGAAAGTGAAGGTTTGTTTCGATATGGTCACTATCGAAGAGAAGCTGATGATATACGCGCTGTAGTTCAATACTTTCGTGGGAAGGATTATGTAGTAACTGCAGTTGTTGGTCACAGTAAAGGGGGGAATGCAGTGATCTTGTATGCTGCAAAATACAGTGATGTTCGTGCAGTTGTAAATATTTCTGGCCGATTTGATCTGGAAAGAGGCATGGAAGGTCGCTTAGGTAAAGATTTTCGACAAAGAATCAAGCGAGATGGATTTATTGACGTTAAGAACAGAAGAGGTACATATCAGTATCGTGTCACCGAAGAAAGTTTGATAGAGCGTCTAACAACTGATATGAGTGCAGCCTGCCAATCGATTAATCAAAATTGCAGGGTTTTGACAGTTCATGGATCAAATGATAAAATTGTTCCTTTTGAAGATGCTTTTGCGTTTGACAAGATTATACCTAATCACAAAGTATCGATCGTGGAAGGAGCTGATCACGAATACACTTTACACCAAAACGAGCTGGCTTCGATCGTGTTGGACTTCATCAAGGAAGATTTTCACCAGGACATGGATCTGTCTAAGCAGTCACCATCTAGCACAAGAGCCGATAAATCTCTCGATTCACGATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Hydrolase_4 Ribosomal_S10

Protein Analysis

291

Amino Acids

32.77

Weight (kDa)

8.34

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 40 - 152 4.3e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 42 - 162 1.5e-07 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 43 - 250 4.5e-06 Alpha/beta hydrolase family
Peptidase_S9 PF00326 93 - 266 1e-05 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 808
AccII CGCG 1 cut(s) 300
AciI CCGC 2 cut(s) 186, 220
AclWI GGATC 3 cut(s) 129, 649, 819
AcoI YGGCCR 2 cut(s) 14, 430
AfaI GTAC 1 cut(s) 532
AfiI CCNNNNNNNGG 1 cut(s) 808
AgsI TTSAA 2 cut(s) 311, 668
AjnI CCWGG 1 cut(s) 801
AleI CACNNNNGTG 1 cut(s) 868
AluBI AGCT 4 cut(s) 88, 287, 728, 760
AluI AGCT 4 cut(s) 88, 287, 728, 760
AlwI GGATC 3 cut(s) 129, 649, 819
AoxI GGCC 2 cut(s) 14, 430
ApeKI GCWGC 2 cut(s) 389, 593
AseI ATTAAT 1 cut(s) 609
Asp700I GAANNNNTTC 2 cut(s) 165, 795
AspLEI GCGC 3 cut(s) 54, 215, 302
AsuHPI GGTGA 5 cut(s) 19, 187, 541, 791, 819
AsuII TTCGAA 1 cut(s) 116
BbvI GCAGC 2 cut(s) 376, 605
BccI CCATC 3 cut(s) 197, 494, 838
BciT130I CCWGG 1 cut(s) 803
BclI TGATCA 1 cut(s) 730
BfaI CTAG 1 cut(s) 834
BfmI CTRYAG 2 cut(s) 303, 342
BfoI RGCGCY 1 cut(s) 216
BisI GCNGC 3 cut(s) 186, 390, 594
BlsI GCNGC 3 cut(s) 187, 391, 595
Bme1390I CCNGG 1 cut(s) 803
BmrFI CCNGG 1 cut(s) 803
BmsI GCATC 2 cut(s) 153, 661
Bpu10I CCTNAGC 1 cut(s) 466
Bpu14I TTCGAA 1 cut(s) 116
Bsa29I ATCGAT 2 cut(s) 605, 713
BsaJI CCNNGG 2 cut(s) 139, 171
Bsc4I CCNNNNNNNGG 1 cut(s) 808
Bse1I ACTGG 1 cut(s) 38
Bse3DI GCAATG 1 cut(s) 180
BseBI CCWGG 1 cut(s) 803
BseCI ATCGAT 2 cut(s) 605, 713
BseDI CCNNGG 2 cut(s) 139, 171
BseGI GGATG 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 808
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 1 cut(s) 38
BseX3I CGGCCG 1 cut(s) 14
BseXI GCAGC 2 cut(s) 376, 605
BsgI GTGCAG 2 cut(s) 432, 612
Bsh1236I CGCG 1 cut(s) 300
Bsh1285I CGRYCG 3 cut(s) 17, 717, 771
BshFI GGCC 2 cut(s) 16, 432
BshVI ATCGAT 2 cut(s) 605, 713
BsiEI CGRYCG 3 cut(s) 17, 717, 771
BslI CCNNNNNNNGG 1 cut(s) 808
BsmI GAATGC 2 cut(s) 165, 376
BsnI GGCC 2 cut(s) 16, 432
Bsp119I TTCGAA 1 cut(s) 116
Bsp143I GATC 8 cut(s) 121, 378, 439, 641, 714, 730, 768, 811
Bsp19I CCATGG 2 cut(s) 139, 171
BspACI CCGC 2 cut(s) 186, 220
BspANI GGCC 2 cut(s) 16, 432
BspCNI CTCAG 1 cut(s) 40
BspDI ATCGAT 2 cut(s) 605, 713
BspFNI CGCG 1 cut(s) 300
BspMAI CTGCAG 1 cut(s) 346
BspPI GGATC 3 cut(s) 129, 649, 819
BspT104I TTCGAA 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 180
BsrI ACTGG 1 cut(s) 38
BssECI CCNNGG 2 cut(s) 139, 171
BssMI GATC 8 cut(s) 121, 378, 439, 641, 714, 730, 768, 811
BssT1I CCWWGG 2 cut(s) 139, 171
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 3 cut(s) 359, 401, 634
Bst6I CTCTTC 2 cut(s) 274, 520
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 598, 762
BstDEI CTNAG 3 cut(s) 27, 466, 819
BstDSI CCRYGG 2 cut(s) 139, 171
BstF5I GGATG 1 cut(s) 144
BstFNI CGCG 1 cut(s) 300
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 3 cut(s) 54, 215, 302
BstKTI GATC 8 cut(s) 124, 381, 442, 644, 717, 733, 771, 814
BstMBI GATC 8 cut(s) 121, 378, 439, 641, 714, 730, 768, 811
BstMCI CGRYCG 3 cut(s) 17, 717, 771
BstMWI GCNNNNNNNGC 1 cut(s) 219
BstNI CCWGG 1 cut(s) 803
BstSCI CCNGG 1 cut(s) 801
BstSFI CTRYAG 2 cut(s) 303, 342
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 376, 605
BstX2I RGATCY 2 cut(s) 121, 811
BstYI RGATCY 2 cut(s) 121, 811
BstZI CGGCCG 1 cut(s) 14
Bsu15I ATCGAT 2 cut(s) 605, 713
BsuRI GGCC 2 cut(s) 16, 432
BsuTUI ATCGAT 2 cut(s) 605, 713
BtgI CCRYGG 2 cut(s) 139, 171
BtsCI GGATG 1 cut(s) 144
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 381, 406
Cac8I GCNNGC 2 cut(s) 598, 762
CfoI GCGC 3 cut(s) 54, 215, 302
ClaI ATCGAT 2 cut(s) 605, 713
CseI GACGC 1 cut(s) 560
Csp6I GTAC 1 cut(s) 531
CviAII CATG 7 cut(s) 77, 104, 140, 172, 454, 638, 808
CviJI RGCY 9 cut(s) 16, 88, 287, 432, 596, 728, 760, 764, 845
CviKI_1 RGCY 9 cut(s) 16, 88, 287, 432, 596, 728, 760, 764, 845
CviQI GTAC 1 cut(s) 531
DdeI CTNAG 3 cut(s) 27, 466, 819
DpnI GATC 8 cut(s) 123, 380, 441, 643, 716, 732, 770, 813
DpnII GATC 8 cut(s) 121, 378, 439, 641, 714, 730, 768, 811
EaeI YGGCCR 2 cut(s) 14, 430
EagI CGGCCG 1 cut(s) 14
Eam1104I CTCTTC 2 cut(s) 274, 520
EarI CTCTTC 2 cut(s) 274, 520
EclXI CGGCCG 1 cut(s) 14
Eco130I CCWWGG 2 cut(s) 139, 171
Eco52I CGGCCG 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 801
EcoT14I CCWWGG 2 cut(s) 139, 171
ErhI CCWWGG 2 cut(s) 139, 171
FaeI CATG 7 cut(s) 80, 107, 143, 175, 457, 641, 811
FatI CATG 7 cut(s) 76, 103, 139, 171, 453, 637, 807
FbaI TGATCA 1 cut(s) 730
Fnu4HI GCNGC 3 cut(s) 186, 390, 594
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 186, 390, 594
FspBI CTAG 1 cut(s) 834
GlaI GCGC 3 cut(s) 53, 214, 301
GluI GCNGC 3 cut(s) 186, 390, 594
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 2 cut(s) 16, 432
HgaI GACGC 1 cut(s) 560
HhaI GCGC 3 cut(s) 54, 215, 302
Hin1II CATG 7 cut(s) 80, 107, 143, 175, 457, 641, 811
Hin6I GCGC 3 cut(s) 52, 213, 300
HinP1I GCGC 3 cut(s) 52, 213, 300
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 5 cut(s) 10, 19, 25, 489, 860
HphI GGTGA 5 cut(s) 19, 187, 541, 791, 819
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 2 cut(s) 24, 30
Hpy188III TCNNGA 5 cut(s) 94, 443, 734, 857, 864
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 6 cut(s) 112, 226, 245, 319, 452, 716
HpyCH4III ACNGT 3 cut(s) 359, 401, 634
HpyCH4IV ACGT 1 cut(s) 513
HpyCH4V TGCA 6 cut(s) 344, 374, 392, 413, 593, 621
HpyF10VI GCNNNNNNNGC 1 cut(s) 219
HpyF3I CTNAG 3 cut(s) 27, 466, 819
HpySE526I ACGT 1 cut(s) 513
Hsp92II CATG 7 cut(s) 80, 107, 143, 175, 457, 641, 811
HspAI GCGC 3 cut(s) 52, 213, 300
Ksp22I TGATCA 1 cut(s) 730
Kzo9I GATC 8 cut(s) 121, 378, 439, 641, 714, 730, 768, 811
LmnI GCTCC 1 cut(s) 725
Lsp1109I GCAGC 2 cut(s) 376, 605
LweI GCATC 2 cut(s) 153, 661
MaeI CTAG 1 cut(s) 834
MaeII ACGT 1 cut(s) 513
MaeIII GTNAC 7 cut(s) 7, 40, 269, 337, 353, 547, 825
MalI GATC 8 cut(s) 123, 380, 441, 643, 716, 732, 770, 813
MboI GATC 8 cut(s) 121, 378, 439, 641, 714, 730, 768, 811
MboII GAAGA 5 cut(s) 291, 537, 566, 680, 803
MflI RGATCY 2 cut(s) 121, 811
MluCI AATT 2 cut(s) 616, 654
MlyI GAGTC 3 cut(s) 4, 13, 19
MmeI TCCRAC 2 cut(s) 47, 756
MnlI CCTC 3 cut(s) 102, 443, 521
MroXI GAANNNNTTC 2 cut(s) 165, 795
MseI TTAA 2 cut(s) 516, 609
MslI CAYNNNNRTG 2 cut(s) 170, 868
MspA1I CMGCKG 1 cut(s) 188
MspR9I CCNGG 1 cut(s) 803
Mva1269I GAATGC 2 cut(s) 165, 376
MvaI CCWGG 1 cut(s) 803
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 1 cut(s) 219
NcoI CCATGG 2 cut(s) 139, 171
NdeII GATC 8 cut(s) 121, 378, 439, 641, 714, 730, 768, 811
NlaIII CATG 7 cut(s) 80, 107, 143, 175, 457, 641, 811
NmuCI GTSAC 5 cut(s) 7, 269, 353, 547, 825
NspV TTCGAA 1 cut(s) 116
OliI CACNNNNGTG 1 cut(s) 868
PctI GAATGC 2 cut(s) 165, 376
PdmI GAANNNNTTC 2 cut(s) 165, 795
PfeI GAWTC 2 cut(s) 489, 860
PflMI CCANNNNNTGG 1 cut(s) 808
PkrI GCNGC 3 cut(s) 187, 391, 595
Ple19I CGATCG 2 cut(s) 717, 771
PleI GAGTC 3 cut(s) 4, 13, 19
PpsI GAGTC 3 cut(s) 4, 13, 19
PshBI ATTAAT 1 cut(s) 609
Psp6I CCWGG 1 cut(s) 801
PspGI CCWGG 1 cut(s) 801
PstI CTGCAG 1 cut(s) 346
PsuI RGATCY 2 cut(s) 121, 811
PvuI CGATCG 2 cut(s) 717, 771
RsaI GTAC 1 cut(s) 532
RsaNI GTAC 1 cut(s) 531
RseI CAYNNNNRTG 2 cut(s) 170, 868
SaqAI TTAA 2 cut(s) 516, 609
SatI GCNGC 3 cut(s) 186, 390, 594
Sau3AI GATC 8 cut(s) 121, 378, 439, 641, 714, 730, 768, 811
SchI GAGTC 3 cut(s) 4, 13, 19
ScrFI CCNGG 1 cut(s) 803
SfaNI GCATC 2 cut(s) 153, 661
SfcI CTRYAG 2 cut(s) 303, 342
SfuI TTCGAA 1 cut(s) 116
SmiMI CAYNNNNRTG 2 cut(s) 170, 868
Sse9I AATT 2 cut(s) 616, 654
SsiI CCGC 2 cut(s) 186, 220
SspI AATATT 1 cut(s) 424
SspMI CTAG 1 cut(s) 834
StyD4I CCNGG 1 cut(s) 801
StyI CCWWGG 2 cut(s) 139, 171
TaaI ACNGT 3 cut(s) 359, 401, 634
TaiI ACGT 1 cut(s) 516
TaqI TCGA 8 cut(s) 116, 262, 277, 481, 605, 713, 767, 858
TasI AATT 2 cut(s) 616, 654
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 2 cut(s) 489, 860
Tru1I TTAA 2 cut(s) 516, 609
Tru9I TTAA 2 cut(s) 516, 609
TscAI CASTG 2 cut(s) 381, 406
TseFI GTSAC 5 cut(s) 7, 269, 353, 547, 825
TseI GCWGC 2 cut(s) 389, 593
Tsp45I GTSAC 5 cut(s) 7, 269, 353, 547, 825
TspDTI ATGAA 2 cut(s) 626, 772
TspRI CASTG 2 cut(s) 381, 406
Van91I CCANNNNNTGG 1 cut(s) 808
VspI ATTAAT 1 cut(s) 609
XmnI GAANNNNTTC 2 cut(s) 165, 795
XspI CTAG 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.