pycom15g05770

PITH domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008315
Physical Location & Seq
Reverse (-)
3497651 .. 3500879
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
ATGCGAATGGCGAAAAATGTAATTTCCCTTGAAAATGCAACGGTTCGCTCCTATTTGTGGTTGCGTCTATTTTCTTCCTTCATCTATTTGTCGTGTGAGAAATTCAGCGATCGTAGGGAAAGAGAGATGGCTTGCTTGCACGATCACAGCTGCGAAGATCACGATTGTTCGTCCGGTTGGACTCTCTACGAGCATATCGACCTCCCCAAGGTTTCTGCTTTGAATGAGGCGGTTCCAGGAAGCGTCAAGTCGGTTTTCAAAGCTTGGGAGCAACGGCTGAATTCTAGTGGGGGACACTTGGAGAGCAATGAGGGTGATCCTGAGTTACTTGTTTACATTCCATTTACATCAGATGTAAAGATCAAGAGCATATCAGTTGTAGGTGGTGTTGACGGAACAAGTCCTTCCAAGATGAGGGCGTTCATAAACAAAGAAGGGATTGACTTTTCAGATGCTCAAGGAATGCAAGCTGTTCAGGAGTGGGATCTGGTCGAAAATTTTCAAGGAACGTTGGAGTACCAGACAAGATATTCCAAATTTCAAAACGTGGCCAGCATCACATTGCATTTTCCCAATAGTTTTGGTGGTGATACAACTAAGATAGAGTACATTGGCTTTAAAGGCGAAGCTACAAAGTTGAAGAGAGATGTTGTCGCAACAATTGTTTACGAACTCAGGCCAAATCCTTCCGATCATAAGACAAAGGCCGAAGGTGGGGGTCTCTCACATATTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH

Protein Analysis

246

Amino Acids

27.51

Weight (kDa)

5.66

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PITH PF06201 54 - 226 1.5e-53 PITH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 230
AclI AACGTT 1 cut(s) 509
AclWI GGATC 2 cut(s) 311, 492
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 4 cut(s) 101, 280, 496, 536
AfaI GTAC 2 cut(s) 518, 608
AfiI CCNNNNNNNGG 4 cut(s) 57, 208, 414, 714
AgsI TTSAA 7 cut(s) 32, 223, 259, 503, 542, 640, 734
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 4 cut(s) 150, 263, 470, 629
AluI AGCT 4 cut(s) 150, 263, 470, 629
Alw26I GTCTC 1 cut(s) 725
AlwI GGATC 2 cut(s) 311, 492
AoxI GGCC 3 cut(s) 549, 677, 705
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 4 cut(s) 101, 280, 496, 536
Asp700I GAANNNNTTC 1 cut(s) 498
AsuHPI GGTGA 2 cut(s) 326, 599
BalI TGGCCA 1 cut(s) 551
BbvI GCAGC 1 cut(s) 137
BccI CCATC 1 cut(s) 121
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 1 cut(s) 237
BcoDI GTCTC 1 cut(s) 725
BfaI CTAG 1 cut(s) 285
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 237
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 237
BmsI GCATC 2 cut(s) 442, 564
BpuEI CTTGAG 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 725
BsaJI CCNNGG 1 cut(s) 207
BsaWI WCCGGW 1 cut(s) 173
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 208, 414, 714
Bse3DI GCAATG 2 cut(s) 313, 560
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 207
BseLI CCNNNNNNNGG 4 cut(s) 57, 208, 414, 714
BseMI GCAATG 2 cut(s) 313, 560
BseMII CTCAG 2 cut(s) 312, 688
BseXI GCAGC 1 cut(s) 137
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 3 cut(s) 551, 679, 707
BsiEI CGRYCG 1 cut(s) 112
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 4 cut(s) 57, 208, 414, 714
BsmAI GTCTC 1 cut(s) 725
BsmFI GGGAC 1 cut(s) 306
BsmI GAATGC 1 cut(s) 468
BsnI GGCC 3 cut(s) 551, 679, 707
Bso31I GGTCTC 1 cut(s) 725
Bsp143I GATC 7 cut(s) 109, 142, 157, 316, 360, 484, 691
BspACI CCGC 1 cut(s) 230
BspANI GGCC 3 cut(s) 551, 679, 707
BspCNI CTCAG 2 cut(s) 313, 687
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 2 cut(s) 311, 492
BspTNI GGTCTC 1 cut(s) 725
BsrDI GCAATG 2 cut(s) 313, 560
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 7 cut(s) 109, 142, 157, 316, 360, 484, 691
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 43
Bst6I CTCTTC 1 cut(s) 635
BstC8I GCNNGC 4 cut(s) 133, 137, 468, 553
BstDEI CTNAG 3 cut(s) 321, 597, 674
BstENI CCTNNNNNAGG 1 cut(s) 206
BstKTI GATC 7 cut(s) 112, 145, 160, 319, 363, 487, 694
BstMAI GTCTC 1 cut(s) 725
BstMBI GATC 7 cut(s) 109, 142, 157, 316, 360, 484, 691
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 1 cut(s) 621
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 1 cut(s) 235
BstV1I GCAGC 1 cut(s) 137
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
BsuRI GGCC 3 cut(s) 551, 679, 707
Cac8I GCNNGC 4 cut(s) 133, 137, 468, 553
CseI GACGC 2 cut(s) 53, 232
Csp6I GTAC 2 cut(s) 517, 607
CviQI GTAC 2 cut(s) 517, 607
DdeI CTNAG 3 cut(s) 321, 597, 674
DpnI GATC 7 cut(s) 111, 144, 159, 318, 362, 486, 693
DpnII GATC 7 cut(s) 109, 142, 157, 316, 360, 484, 691
DraI TTTAAA 1 cut(s) 619
EaeI YGGCCR 1 cut(s) 549
Eam1104I CTCTTC 1 cut(s) 635
EarI CTCTTC 1 cut(s) 635
Eco130I CCWWGG 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 725
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoRI GAATTC 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FaiI YATR 5 cut(s) 195, 371, 425, 696, 729
FaqI GGGAC 1 cut(s) 306
Fnu4HI GCNGC 1 cut(s) 151
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 1 cut(s) 285
GluI GCNGC 1 cut(s) 151
HaeIII GGCC 3 cut(s) 551, 679, 707
HapII CCGG 1 cut(s) 174
HgaI GACGC 2 cut(s) 53, 232
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 1 cut(s) 181
HpaII CCGG 1 cut(s) 174
HphI GGTGA 2 cut(s) 326, 599
Hpy166II GTNNAC 3 cut(s) 334, 391, 667
Hpy188I TCNGA 3 cut(s) 352, 451, 691
Hpy188III TCNNGA 4 cut(s) 161, 320, 364, 476
Hpy8I GTNNAC 3 cut(s) 334, 391, 667
HpyAV CCTTC 5 cut(s) 88, 414, 428, 696, 704
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 2 cut(s) 509, 546
HpyCH4V TGCA 4 cut(s) 38, 139, 466, 565
HpyF10VI GCNNNNNNNGC 1 cut(s) 621
HpyF3I CTNAG 3 cut(s) 321, 597, 674
HpySE526I ACGT 2 cut(s) 509, 546
Kzo9I GATC 7 cut(s) 109, 142, 157, 316, 360, 484, 691
LmnI GCTCC 2 cut(s) 53, 268
LpnPI CCDG 9 cut(s) 187, 222, 249, 333, 461, 473, 533, 565, 661
Lsp1109I GCAGC 1 cut(s) 137
LweI GCATC 2 cut(s) 442, 564
MaeI CTAG 1 cut(s) 285
MaeII ACGT 2 cut(s) 509, 546
MaeIII GTNAC 1 cut(s) 324
MalI GATC 7 cut(s) 111, 144, 159, 318, 362, 486, 693
MboI GATC 7 cut(s) 109, 142, 157, 316, 360, 484, 691
MboII GAAGA 3 cut(s) 66, 167, 652
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 1 cut(s) 484
MlsI TGGCCA 1 cut(s) 551
MluCI AATT 6 cut(s) 21, 101, 280, 496, 536, 660
MluNI TGGCCA 1 cut(s) 551
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 2 cut(s) 158, 492
MnlI CCTC 4 cut(s) 212, 220, 304, 408
Mox20I TGGCCA 1 cut(s) 551
MroXI GAANNNNTTC 1 cut(s) 498
MscI TGGCCA 1 cut(s) 551
MseI TTAA 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 551
MspA1I CMGCKG 1 cut(s) 150
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 237
MunI CAATTG 1 cut(s) 660
Mva1269I GAATGC 1 cut(s) 468
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 621
NdeII GATC 7 cut(s) 109, 142, 157, 316, 360, 484, 691
NlaIV GGNNCC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 1 cut(s) 468
PdmI GAANNNNTTC 1 cut(s) 498
PfoI TCCNGGA 1 cut(s) 235
PkrI GCNGC 1 cut(s) 152
Ple19I CGATCG 1 cut(s) 112
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
Psp1406I AACGTT 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 234
PsuI RGATCY 1 cut(s) 484
PvuI CGATCG 1 cut(s) 112
PvuII CAGCTG 1 cut(s) 150
RsaI GTAC 2 cut(s) 518, 608
RsaNI GTAC 2 cut(s) 517, 607
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 7 cut(s) 109, 142, 157, 316, 360, 484, 691
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 1 cut(s) 237
SfaNI GCATC 2 cut(s) 442, 564
SmlI CTYRAG 1 cut(s) 456
SmoI CTYRAG 1 cut(s) 456
Sse9I AATT 6 cut(s) 21, 101, 280, 496, 536, 660
SsiI CCGC 1 cut(s) 230
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 235
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 2 cut(s) 512, 549
TaqI TCGA 2 cut(s) 198, 492
TasI AATT 6 cut(s) 21, 101, 280, 496, 536, 660
TatI WGTACW 1 cut(s) 606
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TseI GCWGC 1 cut(s) 150
TspDTI ATGAA 2 cut(s) 70, 412
TspGWI ACGGA 1 cut(s) 408
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 4 cut(s) 101, 280, 496, 536
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.