pycom15g07550

Protein ROOT INITIATION DEFECTIVE 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007848
Physical Location & Seq
Forward (+)
4642483 .. 4645464
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1431 bp
ATGTATCCTTACTCTGTCCTAGATTCCAAAACACTCAATAAAAACCCTCGTGAGAACCCCCAATTCACCAATCCAATCCAGTCGAATCCATCTATACTCTCAATGGAGCTGGTGGTGGCCTCGTCGTCTGTCGAAGCCGGCATCGGCTGTTGGGACCTCCGCACCGGCGCCGAGCAGCTCCGCTACAAGCTCTGCGCTTCTCCGCCTCATGGCCTCATTTGCGTCGGCGAACGATTCCTCGCCTCCTCTCAGCTCCGTGAGCCTTCTGCCTCCTCTGGCTCTGTACAGTACTGGTCTTGGTCCAAGCCGCAGGTTGATGTGAAGAGCTTCCCAGAAGAACCGATCAATCCGCTCGCTGCGAACAGCGAAGGGACGTACATTGTTGGCGGTGGCTCATCGGGGAATATCTACTTGTGGGAGGTTAATACTGGTAGACTGCTTAAGAAATGGCATGCTCACTACAGAGGTGTTAGTTGCTTGGCATTTTCGGATGATGGCTCCCTGCTGATTTCGGGGTCGGAGGATGGAGGCATTAGAGTTTGGTCTCTTATAGGATTATTCGATGATTATCAAAGTCAACAGGAAAGCCATCTGTACATGCATAATTTTACGGAGCACACTCTTTGTGTAACGGATGTTGTCACAGGATATGGTGGATGCAAAGCCATTATCGTGTCAGCATCACAGGATCGCACATGTAAGGTTTGGAGTGTAGGAAGTGGGAGATTATTGAGAAATATTGTTTTCCCTACTATAATTGATGCGATTGCACTGGATCCTGGTGAAAATGTCTTCTTTGCCGGTGGTAGAGATGGCAAGATATACGTTGCTGCACTTAATGCTGAGAGCCCACTTAACAGCAAGTATGGGTTGCATATCATCGATTGTTTCTCGAATCACAGCAAAGCTGTTACTTGCCTAGCATATGGCATTACTGGAAATTATTTGCTTTCTGGATCAGAAGATGGCATCGTTCGTGTTTGGGATGCTAGAACTCATAACATTGTTCGTGTGTTCAAACATGCAAAAGGACCAGTGAATAATATTCTAGTCGTTCGACAGCATTTTTCTCAGATGAATTCGTACACGTCCTCGAGAAGGCATGGTTCATCATTACCACCTCCACTGGAAAAGTATACAACAGAGGAAGGCGGAGACATTAGAGCTTTCCTTGGAGGCCCGGCTACTTGCAGTAATTTGACAGAACCATTGTACATCACTGAACAAGTGATGAATAGGCAAATCAAAGAGCTTCAGCAACAAGGATCTGCCTCAGCCGAAATGGAGATTGAGAGACTGAAGCGCGAAAATGAAAGAGCCACACAAATGGCTCAGCAGTGGAGAAAAATGTATGACAACTTGCATCGGTTCTGTGTAAATGAGCTTTTGGATGGTGATCAAGGAGGAGTCTCAAATGGAAATGCAGCTTGA

Protein Analysis

477

Amino Acids

52.44

Weight (kDa)

6.24

Isoelectric Point (pI)

46.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 38 - 238 6.1e-06 WDR55
Beta-prop_THOC3 PF25174 39 - 183 8.7e-11 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 88 - 213 8.6e-08 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 92 - 182 2.2e-13 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 92 - 188 4.2e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 95 - 240 9e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 108 - 276 3.6e-19 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 115 - 246 6e-13 Prp19 WD40 domain
Beta-prop_EML PF23409 119 - 201 2.5e-08 Echinoderm microtubule-associated protein first beta-propeller
NBCH_WD40 PF20426 120 - 253 6.8e-10 Neurobeachin beta propeller domain
Beta-prop_CAF1B_HIR1 PF24105 126 - 265 9.1e-06 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 145 - 182 3.9e-10 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 152 - 342 6.5e-18 WDHD1 first WD40 domain
WD40_Prp19 PF24814 195 - 349 1.9e-13 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 199 - 345 3.4e-16 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 222 - 348 2e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 222 - 346 2.9e-09 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 223 - 349 1.5e-13 THOC3 beta-propeller domain
EIF3I PF24805 223 - 354 1.9e-09 EIF3I
Beta-prop_WDR90_POC16_2nd PF23393 224 - 339 4.1e-06 WDR90/POC16, second beta-propeller
WDR55 PF24796 246 - 356 1.6e-07 WDR55
Beta-prop_WDR3_1st PF25173 254 - 355 1.2e-09 WDR3 first beta-propeller domain
WD40 PF00400 296 - 329 9.5e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 167
AccBSI CCGCTC 1 cut(s) 352
AccI GTMKAC 2 cut(s) 433, 1136
AccII CGCG 1 cut(s) 1305
AciI CCGC 7 cut(s) 160, 181, 203, 308, 350, 387, 1152
AclWI GGATC 5 cut(s) 696, 770, 783, 964, 1273
AcsI RAATTY 1 cut(s) 1078
AcuI CTGAAG 2 cut(s) 1238, 1319
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 6 cut(s) 285, 290, 377, 596, 1085, 1214
AfiI CCNNNNNNNGG 2 cut(s) 209, 1098
AflII CTTAAG 1 cut(s) 440
AflIII ACRYGT 2 cut(s) 695, 1086
AgsI TTSAA 1 cut(s) 1018
AjiI CACGTC 1 cut(s) 1089
AjnI CCWGG 1 cut(s) 778
Alw21I GWGCWC 1 cut(s) 618
Alw26I GTCTC 4 cut(s) 549, 1149, 1288, 1414
AlwI GGATC 5 cut(s) 696, 770, 783, 964, 1273
Ama87I CYCGRG 1 cut(s) 1093
AoxI GGCC 3 cut(s) 117, 211, 1177
ApeKI GCWGC 4 cut(s) 175, 356, 830, 1424
ApoI RAATTY 1 cut(s) 1078
Asp700I GAANNNNTTC 1 cut(s) 326
AspLEI GCGC 3 cut(s) 170, 197, 1305
AspS9I GGNCC 4 cut(s) 154, 300, 1031, 1178
AsuC2I CCSGG 1 cut(s) 1181
AsuHPI GGTGA 3 cut(s) 58, 794, 1406
AvaI CYCGRG 1 cut(s) 1093
AvaII GGWCC 3 cut(s) 154, 300, 1031
BamHI GGATCC 1 cut(s) 775
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 851
BauI CACGAG 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 784
Bbv12I GWGCWC 1 cut(s) 618
BbvCI CCTCAGC 1 cut(s) 1273
BbvI GCAGC 3 cut(s) 187, 343, 817
BccI CCATC 7 cut(s) 97, 488, 518, 597, 806, 959, 1385
BciT130I CCWGG 1 cut(s) 780
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 1 cut(s) 1396
BcnI CCSGG 1 cut(s) 1181
BcoDI GTCTC 4 cut(s) 549, 1149, 1288, 1414
BfaI CTAG 4 cut(s) 20, 920, 990, 1049
BfmI CTRYAG 1 cut(s) 460
BfoI RGCGCY 1 cut(s) 171
BfrI CTTAAG 1 cut(s) 440
BfuAI ACCTGC 1 cut(s) 301
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 5 cut(s) 176, 308, 357, 831, 1425
BlpI GCTNAGC 1 cut(s) 1332
BlsI GCNGC 5 cut(s) 177, 309, 358, 832, 1426
BmcAI AGTACT 1 cut(s) 290
Bme1390I CCNGG 2 cut(s) 780, 1181
Bme18I GGWCC 3 cut(s) 154, 300, 1031
BmeT110I CYCGRG 1 cut(s) 1093
BmgBI CACGTC 1 cut(s) 1089
BmgT120I GGNCC 4 cut(s) 154, 300, 1031, 1178
BmiI GGNNCC 4 cut(s) 155, 169, 499, 777
BmrFI CCNGG 2 cut(s) 780, 1181
BmsI GCATC 7 cut(s) 150, 647, 689, 751, 976, 978, 1372
BpiI GAAGAC 1 cut(s) 784
Bpu10I CCTNAGC 1 cut(s) 1273
Bpu1102I GCTNAGC 1 cut(s) 1332
BpuMI CCSGG 1 cut(s) 1181
Bsa29I ATCGAT 1 cut(s) 882
BsaBI GATNNNNATC 2 cut(s) 567, 1395
BsaHI GRCGYC 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 1 cut(s) 1171
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 1098
Bse118I RCCGGY 3 cut(s) 137, 164, 800
Bse1I ACTGG 7 cut(s) 79, 296, 433, 777, 940, 1034, 1131
Bse8I GATNNNNATC 2 cut(s) 567, 1395
BseBI CCWGG 1 cut(s) 780
BseCI ATCGAT 1 cut(s) 882
BseDI CCNNGG 1 cut(s) 1171
BseGI GGATG 6 cut(s) 496, 529, 640, 662, 991, 1396
BseJI GATNNNNATC 2 cut(s) 567, 1395
BseLI CCNNNNNNNGG 2 cut(s) 209, 1098
BseMII CTCAG 5 cut(s) 263, 834, 1085, 1287, 1346
BseNI ACTGG 7 cut(s) 79, 296, 433, 777, 940, 1034, 1131
BseRI GAGGAG 3 cut(s) 235, 262, 1419
BseXI GCAGC 3 cut(s) 187, 343, 817
BsgI GTGCAG 1 cut(s) 816
Bsh1236I CGCG 1 cut(s) 1305
BshFI GGCC 3 cut(s) 119, 213, 1179
BshNI GGYRCC 1 cut(s) 167
BshVI ATCGAT 1 cut(s) 882
BsiHKAI GWGCWC 1 cut(s) 618
BsiHKCI CYCGRG 1 cut(s) 1093
BsiSI CCGG 4 cut(s) 138, 165, 801, 1181
BslFI GGGAC 2 cut(s) 167, 385
BslI CCNNNNNNNGG 2 cut(s) 209, 1098
BsmAI GTCTC 4 cut(s) 549, 1149, 1288, 1414
BsmFI GGGAC 2 cut(s) 167, 385
BsnI GGCC 3 cut(s) 119, 213, 1179
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 1093
Bsp1286I GDGCHC 2 cut(s) 618, 851
Bsp1407I TGTACA 3 cut(s) 283, 594, 1212
Bsp143I GATC 6 cut(s) 342, 688, 775, 956, 1265, 1396
Bsp1720I GCTNAGC 1 cut(s) 1332
BspACI CCGC 7 cut(s) 160, 181, 203, 308, 350, 387, 1152
BspANI GGCC 3 cut(s) 119, 213, 1179
BspCNI CTCAG 5 cut(s) 262, 835, 1084, 1286, 1345
BspDI ATCGAT 1 cut(s) 882
BspFNI CGCG 1 cut(s) 1305
BspLI GGNNCC 4 cut(s) 155, 169, 499, 777
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 5 cut(s) 696, 770, 783, 964, 1273
BspQI GCTCTTC 1 cut(s) 317
BspT107I GGYRCC 1 cut(s) 167
BspTI CTTAAG 1 cut(s) 440
BspTNI GGTCTC 1 cut(s) 549
BsrBI CCGCTC 1 cut(s) 352
BsrFI RCCGGY 3 cut(s) 137, 164, 800
BsrGI TGTACA 3 cut(s) 283, 594, 1212
BsrI ACTGG 7 cut(s) 79, 296, 433, 777, 940, 1034, 1131
BssAI RCCGGY 3 cut(s) 137, 164, 800
BssECI CCNNGG 1 cut(s) 1171
BssMI GATC 6 cut(s) 342, 688, 775, 956, 1265, 1396
BssNAI GTATAC 1 cut(s) 1137
BssNI GRCGYC 1 cut(s) 168
BssSI CACGAG 1 cut(s) 48
BssT1I CCWWGG 1 cut(s) 1171
Bst1107I GTATAC 1 cut(s) 1137
Bst2BI CACGAG 1 cut(s) 48
Bst2UI CCWGG 1 cut(s) 780
Bst4CI ACNGT 1 cut(s) 288
Bst6I CTCTTC 1 cut(s) 317
BstACI GRCGYC 1 cut(s) 168
BstAFI CTTAAG 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 839
BstAUI TGTACA 3 cut(s) 283, 594, 1212
BstC8I GCNNGC 3 cut(s) 139, 354, 453
BstDEI CTNAG 5 cut(s) 249, 843, 1071, 1273, 1332
BstENI CCTNNNNNAGG 1 cut(s) 1096
BstF5I GGATG 6 cut(s) 496, 529, 640, 662, 991, 1396
BstFNI CGCG 1 cut(s) 1305
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 3 cut(s) 170, 197, 1305
BstKTI GATC 6 cut(s) 345, 691, 778, 959, 1268, 1399
BstMAI GTCTC 4 cut(s) 549, 1149, 1288, 1414
BstMBI GATC 6 cut(s) 342, 688, 775, 956, 1265, 1396
BstMWI GCNNNNNNNGC 3 cut(s) 219, 259, 839
BstNI CCWGG 1 cut(s) 780
BstNSI RCATGY 4 cut(s) 455, 601, 699, 1025
BstSCI CCNGG 2 cut(s) 778, 1179
BstSFI CTRYAG 1 cut(s) 460
BstUI CGCG 1 cut(s) 1305
BstV1I GCAGC 3 cut(s) 187, 343, 817
BstV2I GAAGAC 1 cut(s) 784
BstX2I RGATCY 2 cut(s) 775, 1265
BstXI CCANNNNNNTGG 1 cut(s) 1327
BstYI RGATCY 2 cut(s) 775, 1265
BstZ17I GTATAC 1 cut(s) 1137
Bsu15I ATCGAT 1 cut(s) 882
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 3 cut(s) 119, 213, 1179
BsuTUI ATCGAT 1 cut(s) 882
BtrI CACGTC 1 cut(s) 1089
BtsCI GGATG 6 cut(s) 496, 529, 640, 662, 991, 1396
BtsI GCAGTG 1 cut(s) 1343
BtsIMutI CAGTG 5 cut(s) 770, 1041, 1124, 1218, 1343
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 3 cut(s) 139, 354, 453
CfoI GCGC 3 cut(s) 170, 197, 1305
Cfr10I RCCGGY 3 cut(s) 137, 164, 800
Cfr13I GGNCC 4 cut(s) 154, 300, 1031, 1178
ClaI ATCGAT 1 cut(s) 882
CseI GACGC 1 cut(s) 211
Csp6I GTAC 6 cut(s) 284, 289, 376, 595, 1084, 1213
CviAII CATG 6 cut(s) 209, 452, 598, 696, 1022, 1103
CviQI GTAC 6 cut(s) 284, 289, 376, 595, 1084, 1213
DdeI CTNAG 5 cut(s) 249, 843, 1071, 1273, 1332
DinI GGCGCC 1 cut(s) 169
DpnI GATC 6 cut(s) 344, 690, 777, 958, 1267, 1398
DpnII GATC 6 cut(s) 342, 688, 775, 956, 1265, 1396
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
EciI GGCGGA 2 cut(s) 192, 1167
Eco130I CCWWGG 1 cut(s) 1171
Eco24I GRGCYC 1 cut(s) 851
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 3 cut(s) 154, 300, 1031
Eco57I CTGAAG 2 cut(s) 1238, 1319
Eco88I CYCGRG 1 cut(s) 1093
EcoNI CCTNNNNNAGG 1 cut(s) 1096
EcoO109I RGGNCCY 1 cut(s) 154
EcoRI GAATTC 1 cut(s) 1078
EcoRII CCWGG 1 cut(s) 778
EcoT14I CCWWGG 1 cut(s) 1171
EcoT22I ATGCAT 1 cut(s) 603
EcoT38I GRGCYC 1 cut(s) 851
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 1171
FaeI CATG 6 cut(s) 212, 455, 601, 699, 1025, 1106
FaqI GGGAC 2 cut(s) 167, 385
FatI CATG 6 cut(s) 208, 451, 597, 695, 1021, 1102
FauNDI CATATG 1 cut(s) 925
FbaI TGATCA 1 cut(s) 1396
FblI GTMKAC 2 cut(s) 433, 1136
Fnu4HI GCNGC 5 cut(s) 176, 308, 357, 831, 1425
FokI GGATG 6 cut(s) 503, 536, 647, 669, 998, 1403
FriOI GRGCYC 1 cut(s) 851
Fsp4HI GCNGC 5 cut(s) 176, 308, 357, 831, 1425
FspBI CTAG 4 cut(s) 20, 920, 990, 1049
GlaI GCGC 3 cut(s) 169, 196, 1304
GluI GCNGC 5 cut(s) 176, 308, 357, 831, 1425
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 3 cut(s) 119, 213, 1179
HapII CCGG 4 cut(s) 138, 165, 801, 1181
HgaI GACGC 1 cut(s) 211
HhaI GCGC 3 cut(s) 170, 197, 1305
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 6 cut(s) 212, 455, 601, 699, 1025, 1106
Hin6I GCGC 3 cut(s) 168, 195, 1303
HinP1I GCGC 3 cut(s) 168, 195, 1303
HincII GTYRAC 1 cut(s) 578
HindII GTYRAC 1 cut(s) 578
HinfI GANTC 5 cut(s) 23, 85, 234, 895, 1407
HpaII CCGG 4 cut(s) 138, 165, 801, 1181
HphI GGTGA 3 cut(s) 58, 794, 1406
Hpy166II GTNNAC 4 cut(s) 434, 578, 1086, 1137
Hpy188I TCNGA 4 cut(s) 490, 520, 961, 1074
Hpy188III TCNNGA 4 cut(s) 50, 892, 954, 1095
Hpy8I GTNNAC 4 cut(s) 434, 578, 1086, 1137
Hpy99I CGWCG 2 cut(s) 127, 227
HpyAV CCTTC 4 cut(s) 273, 362, 1092, 1142
HpyCH4III ACNGT 1 cut(s) 288
HpyCH4IV ACGT 3 cut(s) 374, 825, 1088
HpyCH4V TGCA 9 cut(s) 601, 660, 770, 833, 874, 1025, 1191, 1363, 1424
HpyF10VI GCNNNNNNNGC 3 cut(s) 219, 259, 839
HpyF3I CTNAG 5 cut(s) 249, 843, 1071, 1273, 1332
HpySE526I ACGT 3 cut(s) 374, 825, 1088
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 6 cut(s) 212, 455, 601, 699, 1025, 1106
HspAI GCGC 3 cut(s) 168, 195, 1303
KasI GGCGCC 1 cut(s) 167
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 1396
Kzo9I GATC 6 cut(s) 342, 688, 775, 956, 1265, 1396
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 5 cut(s) 106, 183, 258, 503, 613
Lsp1109I GCAGC 3 cut(s) 187, 343, 817
LweI GCATC 7 cut(s) 150, 647, 689, 751, 976, 978, 1372
MaeI CTAG 4 cut(s) 20, 920, 990, 1049
MaeII ACGT 3 cut(s) 374, 825, 1088
MaeIII GTNAC 3 cut(s) 628, 640, 910
MalI GATC 6 cut(s) 344, 690, 777, 958, 1267, 1398
MbiI CCGCTC 1 cut(s) 352
MboI GATC 6 cut(s) 342, 688, 775, 956, 1265, 1396
MboII GAAGA 4 cut(s) 334, 347, 784, 974
MflI RGATCY 2 cut(s) 775, 1265
MhlI GDGCHC 2 cut(s) 618, 851
MluCI AATT 6 cut(s) 62, 604, 756, 940, 1078, 1195
Mly113I GGCGCC 1 cut(s) 168
MlyI GAGTC 1 cut(s) 1416
MmeI TCCRAC 1 cut(s) 498
Mph1103I ATGCAT 1 cut(s) 603
MroNI GCCGGC 1 cut(s) 137
MroXI GAANNNNTTC 1 cut(s) 326
MseI TTAA 4 cut(s) 423, 441, 837, 855
MslI CAYNNNNRTG 2 cut(s) 671, 1325
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 4 cut(s) 138, 165, 801, 1181
MspR9I CCNGG 2 cut(s) 780, 1181
MvaI CCWGG 1 cut(s) 780
MvnI CGCG 1 cut(s) 1305
MwoI GCNNNNNNNGC 3 cut(s) 219, 259, 839
NaeI GCCGGC 1 cut(s) 139
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 1 cut(s) 1181
NdeI CATATG 1 cut(s) 925
NdeII GATC 6 cut(s) 342, 688, 775, 956, 1265, 1396
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 6 cut(s) 212, 455, 601, 699, 1025, 1106
NlaIV GGNNCC 4 cut(s) 155, 169, 499, 777
NmeAIII GCCGAG 1 cut(s) 196
NmuCI GTSAC 1 cut(s) 640
NsiI ATGCAT 1 cut(s) 603
NspI RCATGY 4 cut(s) 455, 601, 699, 1025
PaeI GCATGC 1 cut(s) 455
PaeR7I CTCGAG 1 cut(s) 1093
PciI ACATGT 1 cut(s) 695
PciSI GCTCTTC 1 cut(s) 317
PdiI GCCGGC 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 326
PfeI GAWTC 4 cut(s) 23, 85, 234, 895
PkrI GCNGC 5 cut(s) 177, 309, 358, 832, 1426
PleI GAGTC 1 cut(s) 1415
PluTI GGCGCC 1 cut(s) 171
PpsI GAGTC 1 cut(s) 1415
PpuMI RGGWCCY 1 cut(s) 154
PscI ACATGT 1 cut(s) 695
Psp5II RGGWCCY 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 778
PspGI CCWGG 1 cut(s) 778
PspN4I GGNNCC 4 cut(s) 155, 169, 499, 777
PspPI GGNCC 4 cut(s) 154, 300, 1031, 1178
PspPPI RGGWCCY 1 cut(s) 154
PsuI RGATCY 2 cut(s) 775, 1265
RsaI GTAC 6 cut(s) 285, 290, 377, 596, 1085, 1214
RsaNI GTAC 6 cut(s) 284, 289, 376, 595, 1084, 1213
RseI CAYNNNNRTG 2 cut(s) 671, 1325
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 4 cut(s) 423, 441, 837, 855
SatI GCNGC 5 cut(s) 176, 308, 357, 831, 1425
Sau3AI GATC 6 cut(s) 342, 688, 775, 956, 1265, 1396
Sau96I GGNCC 4 cut(s) 154, 300, 1031, 1178
ScaI AGTACT 1 cut(s) 290
SchI GAGTC 1 cut(s) 1416
ScrFI CCNGG 2 cut(s) 780, 1181
SduI GDGCHC 2 cut(s) 618, 851
SfaNI GCATC 7 cut(s) 150, 647, 689, 751, 976, 978, 1372
SfcI CTRYAG 1 cut(s) 460
SfoI GGCGCC 1 cut(s) 169
Sfr274I CTCGAG 1 cut(s) 1093
SgrAI CRCCGGYG 1 cut(s) 164
SinI GGWCC 3 cut(s) 154, 300, 1031
SlaI CTCGAG 1 cut(s) 1093
SmiMI CAYNNNNRTG 2 cut(s) 671, 1325
SmlI CTYRAG 2 cut(s) 440, 1093
SmoI CTYRAG 2 cut(s) 440, 1093
SphI GCATGC 1 cut(s) 455
Sse9I AATT 6 cut(s) 62, 604, 756, 940, 1078, 1195
SsiI CCGC 7 cut(s) 160, 181, 203, 308, 350, 387, 1152
SspDI GGCGCC 1 cut(s) 167
SspI AATATT 2 cut(s) 739, 1045
SspMI CTAG 4 cut(s) 20, 920, 990, 1049
StyD4I CCNGG 2 cut(s) 778, 1179
StyI CCWWGG 1 cut(s) 1171
TaaI ACNGT 1 cut(s) 288
TaiI ACGT 3 cut(s) 377, 828, 1091
TaqI TCGA 7 cut(s) 83, 132, 561, 882, 893, 1057, 1094
TasI AATT 6 cut(s) 62, 604, 756, 940, 1078, 1195
TatI WGTACW 4 cut(s) 283, 288, 594, 1212
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 4 cut(s) 23, 85, 234, 895
Tru1I TTAA 4 cut(s) 423, 441, 837, 855
Tru9I TTAA 4 cut(s) 423, 441, 837, 855
TscAI CASTG 5 cut(s) 777, 1041, 1131, 1225, 1343
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 4 cut(s) 175, 356, 830, 1424
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 4 cut(s) 1091, 1098, 1247, 1326
TspGWI ACGGA 3 cut(s) 245, 626, 647
TspRI CASTG 5 cut(s) 777, 1041, 1131, 1225, 1343
Vha464I CTTAAG 1 cut(s) 440
VpaK11BI GGWCC 3 cut(s) 154, 300, 1031
XagI CCTNNNNNAGG 1 cut(s) 1096
XapI RAATTY 1 cut(s) 1078
XceI RCATGY 4 cut(s) 455, 601, 699, 1025
XhoI CTCGAG 1 cut(s) 1093
XmiI GTMKAC 2 cut(s) 433, 1136
XmnI GAANNNNTTC 1 cut(s) 326
XspI CTAG 4 cut(s) 20, 920, 990, 1049
ZrmI AGTACT 1 cut(s) 290
Zsp2I ATGCAT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.