pycom15g10120

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010420
Physical Location & Seq
Forward (+)
6584925 .. 6586590
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGGATTTGGGAATGTCGTCGATGCAAGTAAATGATCCTCAAAGAGGGTTCAGTGTGCTTGCTAATGGACCTCTTGACATGAGGATGGATCCTCAGGCAAGTCTGAAAGCTGAAGACGTTTTGAACTCTTGGCCAGATAATGAGGTAGGCCGCATCCTGCGAGACTATGGGGAAGAAAGCAACTGGTTCTCTCTTCAAAATACAATTGTTAGAGCCCGATCACAGGGTGGATTGCATACCACTGGCGATTTAGTAGATCTTATCAAAAGTGCAACTCCACTGAGTCGAGAAGGACGGCAAGGTTGGATTAAAACAGCAACAAGAGTGTTTCAAGCTCTGAGGATAGCCGTGAACGATGAACTGAAGACTCTGGAGGATTCTCTTTATGCTTCTTTTGACTGCCTTGCACCGGGTGGTAGGTTGGCAGTCATCTCTTTCCACAGTTTGGAGGACAGAATTGTGAAACAAACGTTTCTAGACATCATCAACGTCAAAGACAGAGATGTTGACGTTGACATGATTGAAGAAGCAGCGGGGAAGAAAGATTTGAGAAAGATCATGAGCGACATTGATGAAAACGAAGCATGGATCAAACAAACGGTACAGGGCTTGAGGGGAATAATTCTCACTAAGAGACCAATTACACCATCTGAAACGGAAGAGAGCCTCAACAGCCGAGCTAGAAGTGCCAAGCTTAGGGTGATTCAGAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_5

Protein Analysis

239

Amino Acids

26.68

Weight (kDa)

5.4

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_5 PF01795 1 - 237 2.5e-63 MraW methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 445
AciI CCGC 2 cut(s) 151, 533
AclI AACGTT 1 cut(s) 470
AclWI GGATC 4 cut(s) 29, 83, 96, 596
AcoI YGGCCR 1 cut(s) 131
AcuI CTGAAG 2 cut(s) 132, 383
AdeI CACNNNGTG 2 cut(s) 227, 413
AfaI GTAC 1 cut(s) 603
AfiI CCNNNNNNNGG 5 cut(s) 44, 223, 409, 445, 696
AgsI TTSAA 4 cut(s) 124, 197, 332, 524
AluBI AGCT 5 cut(s) 110, 335, 680, 694, 712
AluI AGCT 5 cut(s) 110, 335, 680, 694, 712
Alw26I GTCTC 2 cut(s) 156, 628
AlwI GGATC 4 cut(s) 29, 83, 96, 596
AoxI GGCC 2 cut(s) 131, 148
ApeKI GCWGC 1 cut(s) 530
AspS9I GGNCC 1 cut(s) 68
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 1 cut(s) 712
AvaII GGWCC 1 cut(s) 68
AxyI CCTNAGG 1 cut(s) 93
BalI TGGCCA 1 cut(s) 133
BamHI GGATCC 1 cut(s) 88
BanII GRGCYC 1 cut(s) 217
BbsI GAAGAC 2 cut(s) 120, 371
BbvI GCAGC 1 cut(s) 542
BccI CCATC 2 cut(s) 79, 655
BceAI ACGGC 2 cut(s) 311, 332
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 2 cut(s) 156, 628
BfaI CTAG 2 cut(s) 476, 681
BglII AGATCT 1 cut(s) 256
BisI GCNGC 2 cut(s) 151, 531
BlsI GCNGC 2 cut(s) 152, 532
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 411
BmsI GCATC 2 cut(s) 12, 162
BpiI GAAGAC 2 cut(s) 120, 371
BpmI CTGGAG 1 cut(s) 392
Bpu10I CCTNAGC 1 cut(s) 695
BpuEI CTTGAG 1 cut(s) 631
BpuMI CCSGG 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 628
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 223, 409, 445, 696
Bse1I ACTGG 2 cut(s) 188, 247
Bse21I CCTNAGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 90, 153
BseLI CCNNNNNNNGG 5 cut(s) 44, 223, 409, 445, 696
BseMII CTCAG 3 cut(s) 107, 272, 329
BseNI ACTGG 2 cut(s) 188, 247
BseXI GCAGC 1 cut(s) 542
BshFI GGCC 2 cut(s) 133, 150
BsiSI CCGG 1 cut(s) 410
BslI CCNNNNNNNGG 5 cut(s) 44, 223, 409, 445, 696
BsmAI GTCTC 2 cut(s) 156, 628
BsnI GGCC 2 cut(s) 133, 150
Bso31I GGTCTC 1 cut(s) 628
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 6 cut(s) 34, 88, 218, 256, 555, 588
BspACI CCGC 2 cut(s) 151, 533
BspANI GGCC 2 cut(s) 133, 150
BspCNI CTCAG 3 cut(s) 106, 273, 330
BspHI TCATGA 1 cut(s) 558
BspLI GGNNCC 1 cut(s) 90
BspPI GGATC 4 cut(s) 29, 83, 96, 596
BspTNI GGTCTC 1 cut(s) 628
BsrI ACTGG 2 cut(s) 188, 247
BssMI GATC 6 cut(s) 34, 88, 218, 256, 555, 588
Bst4CI ACNGT 2 cut(s) 443, 601
Bst6I CTCTTC 2 cut(s) 198, 654
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 5 cut(s) 93, 281, 338, 630, 695
BstENI CCTNNNNNAGG 1 cut(s) 42
BstF5I GGATG 2 cut(s) 90, 153
BstKTI GATC 6 cut(s) 37, 91, 221, 259, 558, 591
BstMAI GTCTC 2 cut(s) 156, 628
BstMBI GATC 6 cut(s) 34, 88, 218, 256, 555, 588
BstMWI GCNNNNNNNGC 2 cut(s) 672, 686
BstSCI CCNGG 1 cut(s) 409
BstV1I GCAGC 1 cut(s) 542
BstV2I GAAGAC 2 cut(s) 120, 371
BstX2I RGATCY 2 cut(s) 88, 256
BstYI RGATCY 2 cut(s) 88, 256
Bsu36I CCTNAGG 1 cut(s) 93
BsuRI GGCC 2 cut(s) 133, 150
BtsCI GGATG 2 cut(s) 90, 153
BtsIMutI CAGTG 3 cut(s) 58, 240, 278
Cac8I GCNNGC 1 cut(s) 60
CciI TCATGA 1 cut(s) 558
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 1 cut(s) 602
CviAII CATG 4 cut(s) 79, 517, 559, 585
CviQI GTAC 1 cut(s) 602
DdeI CTNAG 5 cut(s) 93, 281, 338, 630, 695
DpnI GATC 6 cut(s) 36, 90, 220, 258, 557, 590
DpnII GATC 6 cut(s) 34, 88, 218, 256, 555, 588
DraIII CACNNNGTG 2 cut(s) 227, 413
EaeI YGGCCR 1 cut(s) 131
Eam1104I CTCTTC 2 cut(s) 198, 654
EarI CTCTTC 2 cut(s) 198, 654
Eco24I GRGCYC 1 cut(s) 217
Eco31I GGTCTC 1 cut(s) 628
Eco47I GGWCC 1 cut(s) 68
Eco57I CTGAAG 2 cut(s) 132, 383
Eco81I CCTNAGG 1 cut(s) 93
EcoNI CCTNNNNNAGG 1 cut(s) 42
EcoT38I GRGCYC 1 cut(s) 217
FaeI CATG 4 cut(s) 82, 520, 562, 588
FaiI YATR 7 cut(s) 80, 168, 237, 387, 518, 560, 586
FatI CATG 4 cut(s) 78, 516, 558, 584
FauI CCCGC 1 cut(s) 526
Fnu4HI GCNGC 2 cut(s) 151, 531
FokI GGATG 2 cut(s) 97, 140
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 2 cut(s) 151, 531
FspBI CTAG 2 cut(s) 476, 681
GluI GCNGC 2 cut(s) 151, 531
GsuI CTGGAG 1 cut(s) 392
HaeIII GGCC 2 cut(s) 133, 150
HapII CCGG 1 cut(s) 410
Hin1II CATG 4 cut(s) 82, 520, 562, 588
HincII GTYRAC 2 cut(s) 508, 514
HindII GTYRAC 2 cut(s) 508, 514
HindIII AAGCTT 2 cut(s) 692, 710
HinfI GANTC 4 cut(s) 283, 367, 377, 703
HpaII CCGG 1 cut(s) 410
HphI GGTGA 1 cut(s) 712
Hpy166II GTNNAC 3 cut(s) 352, 508, 514
Hpy188I TCNGA 4 cut(s) 105, 339, 652, 708
Hpy188III TCNNGA 5 cut(s) 74, 287, 371, 476, 559
Hpy8I GTNNAC 3 cut(s) 352, 508, 514
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 1 cut(s) 284
HpyCH4III ACNGT 2 cut(s) 443, 601
HpyCH4IV ACGT 4 cut(s) 117, 470, 489, 510
HpyCH4V TGCA 4 cut(s) 25, 235, 272, 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 672, 686
HpyF3I CTNAG 5 cut(s) 93, 281, 338, 630, 695
HpySE526I ACGT 4 cut(s) 117, 470, 489, 510
Hsp92II CATG 4 cut(s) 82, 520, 562, 588
Kzo9I GATC 6 cut(s) 34, 88, 218, 256, 555, 588
LpnPI CCDG 9 cut(s) 80, 147, 169, 170, 209, 228, 356, 423, 590
Lsp1109I GCAGC 1 cut(s) 542
LweI GCATC 2 cut(s) 12, 162
MaeI CTAG 2 cut(s) 476, 681
MaeII ACGT 4 cut(s) 117, 470, 489, 510
MalI GATC 6 cut(s) 36, 90, 220, 258, 557, 590
MboI GATC 6 cut(s) 34, 88, 218, 256, 555, 588
MboII GAAGA 7 cut(s) 125, 185, 185, 376, 536, 550, 671
MfeI CAATTG 1 cut(s) 204
MflI RGATCY 2 cut(s) 88, 256
MhlI GDGCHC 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 133
MluCI AATT 4 cut(s) 204, 456, 621, 639
MluNI TGGCCA 1 cut(s) 133
MlyI GAGTC 2 cut(s) 292, 361
MmeI TCCRAC 1 cut(s) 284
Mox20I TGGCCA 1 cut(s) 133
MscI TGGCCA 1 cut(s) 133
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 83
Msp20I TGGCCA 1 cut(s) 133
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 1 cut(s) 410
MspR9I CCNGG 1 cut(s) 411
MunI CAATTG 1 cut(s) 204
MwoI GCNNNNNNNGC 2 cut(s) 672, 686
NciI CCSGG 1 cut(s) 411
NdeII GATC 6 cut(s) 34, 88, 218, 256, 555, 588
NlaIII CATG 4 cut(s) 82, 520, 562, 588
NlaIV GGNNCC 1 cut(s) 90
NmeAIII GCCGAG 1 cut(s) 701
PagI TCATGA 1 cut(s) 558
PfeI GAWTC 2 cut(s) 377, 703
PflMI CCANNNNNTGG 1 cut(s) 445
PkrI GCNGC 2 cut(s) 152, 532
PleI GAGTC 2 cut(s) 291, 361
PpsI GAGTC 2 cut(s) 291, 361
Psp1406I AACGTT 1 cut(s) 470
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 1 cut(s) 68
PsuI RGATCY 2 cut(s) 88, 256
RsaI GTAC 1 cut(s) 603
RsaNI GTAC 1 cut(s) 602
RseI CAYNNNNRTG 1 cut(s) 83
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 2 cut(s) 151, 531
Sau3AI GATC 6 cut(s) 34, 88, 218, 256, 555, 588
Sau96I GGNCC 1 cut(s) 68
SchI GAGTC 2 cut(s) 292, 361
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 1 cut(s) 217
SfaNI GCATC 2 cut(s) 12, 162
SinI GGWCC 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
Sse9I AATT 4 cut(s) 204, 456, 621, 639
SsiI CCGC 2 cut(s) 151, 533
SspMI CTAG 2 cut(s) 476, 681
StyD4I CCNGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 443, 601
TaiI ACGT 4 cut(s) 120, 473, 492, 513
TaqI TCGA 2 cut(s) 20, 286
TasI AATT 4 cut(s) 204, 456, 621, 639
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 377, 703
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 3 cut(s) 58, 247, 285
TseI GCWGC 1 cut(s) 530
TspDTI ATGAA 2 cut(s) 372, 588
TspGWI ACGGA 1 cut(s) 671
TspRI CASTG 3 cut(s) 58, 247, 285
Van91I CCANNNNNTGG 1 cut(s) 445
VpaK11BI GGWCC 1 cut(s) 68
XagI CCTNNNNNAGG 1 cut(s) 42
XbaI TCTAGA 1 cut(s) 475
XspI CTAG 2 cut(s) 476, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.