pycom15g10610

protein phosphatase 2C 22

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008007
Physical Location & Seq
Forward (+)
7076185 .. 7077974
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 780 bp
ATGGAGGGAAGTAAAGTGGAGAATGGAAGCTCAAGCGAGGGTCGGCCTCCGAACCCTCTTTCGACCGCTGCGGCCTACTGGCAGTGTGGCTTTGGCCCCGCCGATGGCGTTCCAGTGCCGTGCAAGAAATCACTCGTTCGACACCCGTCTCTTATGAAGACGAAAACAAAAGAGATCAAAGTTGAGCCGGGAGTTCAAGCAGAGGACTTTGAATCTAATTTCATTCCAATTGTCCGCTCCGGAGCATGGGCTGACATTGGGTCTCGTCCAAACATGGAAGATGTGTATGTCTGCGTGGACAATTTTATTGACGATTATGGGCTCAAGGATCATACCGATGGCCCAAGCGCCTTCTATGGGGTGTTTGATGGACATGGAGGAAAGCATGCGGCTGATTTTGCTTCATCCCATCTGCCGAAGTTCATTTTGGAGAATGAAGACTTCCCTACAGATATTGAACGGGTTGTCACTTCAGCATTTCTGCATACTGACACTGCCTTTGAGGAAGCTTGCACCTTGGATGCCAAACTTGCTTCTGGTACCACTGCATTGACAGCTCTTCTCATTGGAAGGTTGTTGGTGGTTGCAAATGCTGGAGATTGTCGAGCAGTGCTGTGCCGCCGTGGAAAAGCAATTGAAATGTCAAGAGATCGAACAAACCAATTTGCAGCAAAGAGAAGCAACGGATCGAGGCATCTGGAGGACATGTGTATGATGGTTACCTTAATGGGCAACTTAACGTTGCTCGTGCCTTGGGAGACTGGCATATGGAAGGGATGA

Protein Analysis

260

Amino Acids

28.14

Weight (kDa)

5.52

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 88 - 218 4.4e-24 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 539
AccB1I GGYRCC 1 cut(s) 539
AccBSI CCGCTC 1 cut(s) 237
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 6 cut(s) 66, 71, 99, 235, 389, 619
AclI AACGTT 1 cut(s) 740
AclWI GGATC 2 cut(s) 336, 694
AcuI CTGAAG 1 cut(s) 456
AfaI GTAC 1 cut(s) 541
AfiI CCNNNNNNNGG 3 cut(s) 104, 246, 357
AflIII ACRYGT 1 cut(s) 705
AgsI TTSAA 4 cut(s) 197, 212, 458, 638
AhdI GACNNNNNGTC 1 cut(s) 259
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 3 cut(s) 30, 509, 557
AluI AGCT 3 cut(s) 30, 509, 557
Alw26I GTCTC 3 cut(s) 153, 267, 752
AlwI GGATC 2 cut(s) 336, 694
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 4 cut(s) 44, 72, 94, 340
ApeKI GCWGC 2 cut(s) 68, 668
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
Asp718I GGTACC 1 cut(s) 539
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 2 cut(s) 95, 341
AsuC2I CCSGG 1 cut(s) 189
BanI GGYRCC 1 cut(s) 539
BanII GRGCYC 1 cut(s) 324
BauI CACGAG 1 cut(s) 746
BbsI GAAGAC 2 cut(s) 164, 444
BbvI GCAGC 2 cut(s) 55, 680
BccI CCATC 5 cut(s) 98, 332, 362, 417, 709
BceAI ACGGC 2 cut(s) 103, 606
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 3 cut(s) 153, 267, 752
BfmI CTRYAG 1 cut(s) 447
BfoI RGCGCY 1 cut(s) 351
BisI GCNGC 5 cut(s) 69, 72, 390, 619, 669
BlsI GCNGC 5 cut(s) 70, 73, 391, 620, 670
Bme1390I CCNGG 1 cut(s) 189
BmeRI GACNNNNNGTC 1 cut(s) 259
BmgT120I GGNCC 2 cut(s) 95, 341
BmiI GGNNCC 2 cut(s) 97, 541
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 2 cut(s) 511, 703
BoxI GACNNNNGTC 1 cut(s) 145
BpiI GAAGAC 2 cut(s) 164, 444
BpmI CTGGAG 2 cut(s) 615, 719
BpuEI CTTGAG 2 cut(s) 16, 308
BpuMI CCSGG 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 516, 622, 752
BsaWI WCCGGW 1 cut(s) 239
BsaXI ACNNNNNCTCC 2 cut(s) 692, 722
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 246, 357
Bse1I ACTGG 3 cut(s) 83, 113, 766
BseAI TCCGGA 1 cut(s) 239
BseDI CCNNGG 3 cut(s) 516, 622, 752
BseGI GGATG 2 cut(s) 404, 526
BseLI CCNNNNNNNGG 3 cut(s) 104, 246, 357
BseNI ACTGG 3 cut(s) 83, 113, 766
BseXI GCAGC 2 cut(s) 55, 680
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 4 cut(s) 46, 74, 96, 342
BshNI GGYRCC 1 cut(s) 539
BsiEI CGRYCG 1 cut(s) 66
BsiSI CCGG 2 cut(s) 188, 240
BslI CCNNNNNNNGG 3 cut(s) 104, 246, 357
BsmAI GTCTC 3 cut(s) 153, 267, 752
BsmBI CGTCTC 1 cut(s) 153
BsnI GGCC 4 cut(s) 46, 74, 96, 342
Bso31I GGTCTC 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 324
Bsp13I TCCGGA 1 cut(s) 239
Bsp143I GATC 4 cut(s) 174, 328, 649, 686
BspACI CCGC 6 cut(s) 66, 71, 99, 235, 389, 619
BspANI GGCC 4 cut(s) 46, 74, 96, 342
BspEI TCCGGA 1 cut(s) 239
BspLI GGNNCC 2 cut(s) 97, 541
BspPI GGATC 2 cut(s) 336, 694
BspQI GCTCTTC 1 cut(s) 564
BspT107I GGYRCC 1 cut(s) 539
BspTNI GGTCTC 1 cut(s) 267
BsrBI CCGCTC 1 cut(s) 237
BsrI ACTGG 3 cut(s) 83, 113, 766
BssECI CCNNGG 3 cut(s) 516, 622, 752
BssMI GATC 4 cut(s) 174, 328, 649, 686
BssSI CACGAG 1 cut(s) 746
BssT1I CCWWGG 2 cut(s) 516, 752
Bst2BI CACGAG 1 cut(s) 746
Bst6I CTCTTC 1 cut(s) 564
BstC8I GCNNGC 2 cut(s) 387, 511
BstDSI CCRYGG 1 cut(s) 622
BstEII GGTNACC 1 cut(s) 718
BstF5I GGATG 2 cut(s) 404, 526
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 4 cut(s) 177, 331, 652, 689
BstMAI GTCTC 3 cut(s) 153, 267, 752
BstMBI GATC 4 cut(s) 174, 328, 649, 686
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 3 cut(s) 398, 530, 554
BstNSI RCATGY 2 cut(s) 389, 709
BstPAI GACNNNNGTC 1 cut(s) 145
BstPI GGTNACC 1 cut(s) 718
BstSCI CCNGG 1 cut(s) 187
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 2 cut(s) 55, 680
BstV2I GAAGAC 2 cut(s) 164, 444
BsuRI GGCC 4 cut(s) 46, 74, 96, 342
BtgI CCRYGG 1 cut(s) 622
BtsCI GGATG 2 cut(s) 404, 526
BtsI GCAGTG 4 cut(s) 89, 492, 543, 615
BtsIMutI CAGTG 5 cut(s) 89, 120, 492, 543, 615
Cac8I GCNNGC 2 cut(s) 387, 511
CfoI GCGC 1 cut(s) 350
Cfr13I GGNCC 2 cut(s) 95, 341
Csp6I GTAC 1 cut(s) 540
CviAII CATG 5 cut(s) 246, 274, 374, 386, 706
CviQI GTAC 1 cut(s) 540
DpnI GATC 4 cut(s) 176, 330, 651, 688
DpnII GATC 4 cut(s) 174, 328, 649, 686
DriI GACNNNNNGTC 1 cut(s) 259
Eam1104I CTCTTC 1 cut(s) 564
Eam1105I GACNNNNNGTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 564
Eco130I CCWWGG 2 cut(s) 516, 752
Eco24I GRGCYC 1 cut(s) 324
Eco31I GGTCTC 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 456
Eco91I GGTNACC 1 cut(s) 718
EcoO65I GGTNACC 1 cut(s) 718
EcoT14I CCWWGG 2 cut(s) 516, 752
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 2 cut(s) 516, 752
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 5 cut(s) 249, 277, 377, 389, 709
FatI CATG 5 cut(s) 245, 273, 373, 385, 705
FauI CCCGC 1 cut(s) 106
FauNDI CATATG 1 cut(s) 767
Fnu4HI GCNGC 5 cut(s) 69, 72, 390, 619, 669
FokI GGATG 2 cut(s) 391, 533
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 5 cut(s) 69, 72, 390, 619, 669
GlaI GCGC 1 cut(s) 349
GluI GCNGC 5 cut(s) 69, 72, 390, 619, 669
GsuI CTGGAG 2 cut(s) 615, 719
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 4 cut(s) 46, 74, 96, 342
HapII CCGG 2 cut(s) 188, 240
HhaI GCGC 1 cut(s) 350
Hin1II CATG 5 cut(s) 249, 277, 377, 389, 709
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HindIII AAGCTT 1 cut(s) 507
HinfI GANTC 1 cut(s) 212
HpaII CCGG 2 cut(s) 188, 240
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 1 cut(s) 51
Hpy188III TCNNGA 3 cut(s) 240, 645, 698
Hpy8I GTNNAC 1 cut(s) 298
HpyAV CCTTC 3 cut(s) 361, 564, 766
HpyCH4IV ACGT 1 cut(s) 740
HpyCH4V TGCA 6 cut(s) 123, 484, 513, 548, 587, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 398, 530, 554
HpySE526I ACGT 1 cut(s) 740
Hsp92II CATG 5 cut(s) 249, 277, 377, 389, 709
HspAI GCGC 1 cut(s) 348
Kpn2I TCCGGA 1 cut(s) 239
KpnI GGTACC 1 cut(s) 543
Kzo9I GATC 4 cut(s) 174, 328, 649, 686
LguI GCTCTTC 1 cut(s) 564
LmnI GCTCC 2 cut(s) 242, 242
LpnPI CCDG 8 cut(s) 64, 126, 201, 253, 522, 579, 683, 747
Lsp1109I GCAGC 2 cut(s) 55, 680
LweI GCATC 2 cut(s) 511, 703
MaeII ACGT 1 cut(s) 740
MaeIII GTNAC 2 cut(s) 466, 718
MalI GATC 4 cut(s) 176, 330, 651, 688
MbiI CCGCTC 1 cut(s) 237
MboI GATC 4 cut(s) 174, 328, 649, 686
MboII GAAGA 4 cut(s) 169, 290, 449, 551
MfeI CAATTG 2 cut(s) 228, 633
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 5 cut(s) 217, 228, 301, 633, 662
MnlI CCTC 8 cut(s) 31, 57, 66, 196, 371, 496, 684, 694
MroI TCCGGA 1 cut(s) 239
MseI TTAA 2 cut(s) 725, 737
MslI CAYNNNNRTG 2 cut(s) 336, 710
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 2 cut(s) 188, 240
MspR9I CCNGG 1 cut(s) 189
MunI CAATTG 2 cut(s) 228, 633
MwoI GCNNNNNNNGC 3 cut(s) 398, 530, 554
NciI CCSGG 1 cut(s) 189
NdeI CATATG 1 cut(s) 767
NdeII GATC 4 cut(s) 174, 328, 649, 686
NlaIII CATG 5 cut(s) 249, 277, 377, 389, 709
NlaIV GGNNCC 2 cut(s) 97, 541
NmuCI GTSAC 1 cut(s) 466
NspI RCATGY 2 cut(s) 389, 709
PaeI GCATGC 1 cut(s) 389
PciI ACATGT 1 cut(s) 705
PciSI GCTCTTC 1 cut(s) 564
PfeI GAWTC 1 cut(s) 212
PkrI GCNGC 5 cut(s) 70, 73, 391, 620, 670
PscI ACATGT 1 cut(s) 705
PshAI GACNNNNGTC 1 cut(s) 145
Psp1406I AACGTT 1 cut(s) 740
PspEI GGTNACC 1 cut(s) 718
PspN4I GGNNCC 2 cut(s) 97, 541
PspPI GGNCC 2 cut(s) 95, 341
RsaI GTAC 1 cut(s) 541
RsaNI GTAC 1 cut(s) 540
RseI CAYNNNNRTG 2 cut(s) 336, 710
SapI GCTCTTC 1 cut(s) 564
SaqAI TTAA 2 cut(s) 725, 737
SatI GCNGC 5 cut(s) 69, 72, 390, 619, 669
Sau3AI GATC 4 cut(s) 174, 328, 649, 686
Sau96I GGNCC 2 cut(s) 95, 341
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 1 cut(s) 324
SetI ASST 7 cut(s) 32, 511, 518, 559, 575, 725, 743
SfaNI GCATC 2 cut(s) 511, 703
SfcI CTRYAG 1 cut(s) 447
SmiMI CAYNNNNRTG 2 cut(s) 336, 710
SmlI CTYRAG 2 cut(s) 31, 323
SmoI CTYRAG 2 cut(s) 31, 323
SphI GCATGC 1 cut(s) 389
Sse9I AATT 5 cut(s) 217, 228, 301, 633, 662
SsiI CCGC 6 cut(s) 66, 71, 99, 235, 389, 619
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 2 cut(s) 516, 752
TaiI ACGT 1 cut(s) 743
TaqI TCGA 5 cut(s) 62, 139, 604, 652, 689
TasI AATT 5 cut(s) 217, 228, 301, 633, 662
TauI GCSGC 3 cut(s) 74, 392, 621
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 2 cut(s) 725, 737
Tru9I TTAA 2 cut(s) 725, 737
TscAI CASTG 5 cut(s) 89, 120, 499, 550, 615
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 2 cut(s) 68, 668
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 5 cut(s) 170, 211, 393, 412, 450
TspGWI ACGGA 1 cut(s) 699
TspRI CASTG 5 cut(s) 89, 120, 499, 550, 615
XceI RCATGY 2 cut(s) 389, 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.