pycom15g11380

ATP synthase B/B' CF(0)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009777
Physical Location & Seq
Forward (+)
7700203 .. 7702225
2023 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 855 bp
ATGTCGTATAGCGCGCTGAGAATTGACGATGATGATTTTGTCCCAGTTGGGCAGTCGTCGATCGAACGGCCTCGCCGCTCCAATTCTCTGCCGTTTGTGCCGTTGCTGCTCGTCGATGGCTTCTCCCGCAAGAGCTTGTCGTACTCCGCGCTTCCTCAGGAGCCTCTCCGGCTCTCCGTTCTCAAATTGGATGGCTCTTGCTTCGATATTCAAGTTTCCATGACAGCCACTATTTTGGAATTAAAGCAGGCAGTCGAGGCTGTTTTCAGTCACATGCCACAGAGGGGACCAGGAAAAATTTCATGGCCACATGTGTGGGGTCATTTCTGCTTGTGCTACGATAATCAGAAGCTACTTGTTGAAACTGATCATATCAAACTTTATGGCATCAAAGACGGTGATCAGCTGCATTTTGTCCGGCATGTTTCAATCAACTACAGCATGACAAAGAGCCAATCATCAAAGAAGGGGTTCCTTGCTTTAAAACAAAACACGATGTCATTGTCGCGATCATTGAGCTTTCAAGAGGAAGACCCGACTGACATTGAGAAAGAAGAGAATGGCAAAGACGTAGAACAGAATGACGAGGTATGTTATGATTCTGATGACATCAAGAATCCAAAGTCCCCGCATTGTGATGATGAAGTTTCTATCCAACACAATGAGAGCACACTGCCCTTCTTATTGGGAGAATGGTTTCCATACTCCAAACTGTCAGCTGTTGGAACATCTAAAAGAACTTGTGCGCCAAGCATAGCCAGTGGGTTGTTGCTTGGGTTTAGGAAGATATTCGGGTTTTGTTTCGAAAAACGCGAAAAACGGTATAGTAGAAGGGATACCTGGAGAACAGATTAG

Protein Analysis

285

Amino Acids

32.35

Weight (kDa)

6.26

Isoelectric Point (pI)

52.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_4 PF18036 56 - 142 3.4e-26 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 78
AccII CGCG 4 cut(s) 14, 149, 508, 813
AciI CCGC 4 cut(s) 76, 127, 147, 629
AcoI YGGCCR 1 cut(s) 305
AcsI RAATTY 1 cut(s) 297
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 310
AgsI TTSAA 4 cut(s) 212, 362, 429, 524
AjnI CCWGG 2 cut(s) 289, 839
AleI CACNNNNGTG 1 cut(s) 313
AluBI AGCT 5 cut(s) 135, 352, 406, 519, 719
AluI AGCT 5 cut(s) 135, 352, 406, 519, 719
Alw21I GWGCWC 1 cut(s) 671
AoxI GGCC 2 cut(s) 68, 305
ApeKI GCWGC 2 cut(s) 106, 406
ApoI RAATTY 1 cut(s) 297
Asp700I GAANNNNTTC 4 cut(s) 298, 470, 696, 788
AspLEI GCGC 4 cut(s) 14, 16, 151, 748
AspS9I GGNCC 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 410
AsuII TTCGAA 1 cut(s) 804
AvaII GGWCC 1 cut(s) 287
AxyI CCTNAGG 1 cut(s) 156
BalI TGGCCA 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 671
BbvI GCAGC 2 cut(s) 93, 393
BccI CCATC 2 cut(s) 110, 185
BceAI ACGGC 3 cut(s) 76, 83, 85
BciT130I CCWGG 2 cut(s) 291, 841
BciVI GTATCC 1 cut(s) 829
BclI TGATCA 2 cut(s) 367, 400
BfmI CTRYAG 1 cut(s) 436
BfuI GTATCC 1 cut(s) 829
BglI GCCNNNNNGGC 1 cut(s) 169
BisI GCNGC 3 cut(s) 76, 107, 407
BlsI GCNGC 3 cut(s) 77, 108, 408
Bme1390I CCNGG 2 cut(s) 291, 841
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 3 cut(s) 162, 288, 473
BmrFI CCNGG 2 cut(s) 291, 841
BmrI ACTGGG 1 cut(s) 38
BmsI GCATC 1 cut(s) 396
BmuI ACTGGG 1 cut(s) 38
BpiI GAAGAC 1 cut(s) 537
Bpu14I TTCGAA 1 cut(s) 804
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse1I ACTGG 2 cut(s) 44, 759
Bse21I CCTNAGG 1 cut(s) 156
BseBI CCWGG 2 cut(s) 291, 841
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMII CTCAG 2 cut(s) 8, 170
BseNI ACTGG 2 cut(s) 44, 759
BsePI GCGCGC 1 cut(s) 12
BseXI GCAGC 2 cut(s) 93, 393
Bsh1236I CGCG 4 cut(s) 14, 149, 508, 813
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 2 cut(s) 70, 307
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 671
BsiSI CCGG 2 cut(s) 169, 418
BslFI GGGAC 3 cut(s) 26, 300, 610
BslI CCNNNNNNNGG 1 cut(s) 284
BsmFI GGGAC 3 cut(s) 26, 300, 610
BsnI GGCC 2 cut(s) 70, 307
Bsp119I TTCGAA 1 cut(s) 804
Bsp1286I GDGCHC 1 cut(s) 671
Bsp143I GATC 4 cut(s) 60, 367, 400, 509
Bsp68I TCGCGA 1 cut(s) 508
BspACI CCGC 4 cut(s) 76, 127, 147, 629
BspANI GGCC 2 cut(s) 70, 307
BspCNI CTCAG 2 cut(s) 9, 169
BspFNI CGCG 4 cut(s) 14, 149, 508, 813
BspLI GGNNCC 3 cut(s) 162, 288, 473
BspT104I TTCGAA 1 cut(s) 804
BsrBI CCGCTC 1 cut(s) 78
BsrI ACTGG 2 cut(s) 44, 759
BssHII GCGCGC 1 cut(s) 12
BssMI GATC 4 cut(s) 60, 367, 400, 509
Bst2UI CCWGG 2 cut(s) 291, 841
Bst4CI ACNGT 3 cut(s) 398, 714, 822
Bst6I CTCTTC 1 cut(s) 549
BstBI TTCGAA 1 cut(s) 804
BstC8I GCNNGC 2 cut(s) 14, 249
BstDEI CTNAG 2 cut(s) 17, 156
BstF5I GGATG 1 cut(s) 196
BstFNI CGCG 4 cut(s) 14, 149, 508, 813
BstHHI GCGC 4 cut(s) 14, 16, 151, 748
BstKTI GATC 4 cut(s) 63, 370, 403, 512
BstMBI GATC 4 cut(s) 60, 367, 400, 509
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 5 cut(s) 97, 106, 126, 169, 257
BstNI CCWGG 2 cut(s) 291, 841
BstNSI RCATGY 3 cut(s) 277, 314, 425
BstSCI CCNGG 2 cut(s) 289, 839
BstSFI CTRYAG 1 cut(s) 436
BstUI CGCG 4 cut(s) 14, 149, 508, 813
BstV1I GCAGC 2 cut(s) 93, 393
BstV2I GAAGAC 1 cut(s) 537
BstXI CCANNNNNNTGG 2 cut(s) 235, 315
Bsu36I CCTNAGG 1 cut(s) 156
BsuI GTATCC 1 cut(s) 829
BsuRI GGCC 2 cut(s) 70, 307
BtsCI GGATG 1 cut(s) 196
BtsI GCAGTG 1 cut(s) 671
BtsIMutI CAGTG 2 cut(s) 671, 766
BtuMI TCGCGA 1 cut(s) 508
Cac8I GCNNGC 2 cut(s) 14, 249
CfoI GCGC 4 cut(s) 14, 16, 151, 748
Cfr13I GGNCC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 142
CviAII CATG 6 cut(s) 220, 274, 303, 311, 422, 442
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 2 cut(s) 17, 156
DpnI GATC 4 cut(s) 62, 369, 402, 511
DpnII GATC 4 cut(s) 60, 367, 400, 509
DraI TTTAAA 1 cut(s) 483
EaeI YGGCCR 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 287
Eco81I CCTNAGG 1 cut(s) 156
EcoRII CCWGG 2 cut(s) 289, 839
FaeI CATG 6 cut(s) 223, 277, 306, 314, 425, 445
FaqI GGGAC 3 cut(s) 26, 300, 610
FatI CATG 6 cut(s) 219, 273, 302, 310, 421, 441
FauI CCCGC 2 cut(s) 134, 636
FbaI TGATCA 2 cut(s) 367, 400
Fnu4HI GCNGC 3 cut(s) 76, 107, 407
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 3 cut(s) 76, 107, 407
GlaI GCGC 4 cut(s) 13, 15, 150, 747
GluI GCNGC 3 cut(s) 76, 107, 407
HaeIII GGCC 2 cut(s) 70, 307
HapII CCGG 2 cut(s) 169, 418
HhaI GCGC 4 cut(s) 14, 16, 151, 748
Hin1II CATG 6 cut(s) 223, 277, 306, 314, 425, 445
Hin6I GCGC 4 cut(s) 12, 14, 149, 746
HinP1I GCGC 4 cut(s) 12, 14, 149, 746
HinfI GANTC 2 cut(s) 599, 616
HpaII CCGG 2 cut(s) 169, 418
HphI GGTGA 1 cut(s) 410
Hpy188I TCNGA 2 cut(s) 348, 604
Hpy188III TCNNGA 4 cut(s) 158, 507, 524, 613
Hpy99I CGWCG 2 cut(s) 61, 116
HpyAV CCTTC 3 cut(s) 460, 688, 825
HpyCH4III ACNGT 3 cut(s) 398, 714, 822
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 1 cut(s) 409
HpyF10VI GCNNNNNNNGC 5 cut(s) 97, 106, 126, 169, 257
HpyF3I CTNAG 2 cut(s) 17, 156
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 6 cut(s) 223, 277, 306, 314, 425, 445
HspAI GCGC 4 cut(s) 12, 14, 149, 746
Ksp22I TGATCA 2 cut(s) 367, 400
Kzo9I GATC 4 cut(s) 60, 367, 400, 509
LmnI GCTCC 2 cut(s) 83, 160
LpnPI CCDG 9 cut(s) 57, 143, 182, 233, 276, 303, 431, 772, 826
Lsp1109I GCAGC 2 cut(s) 93, 393
LweI GCATC 1 cut(s) 396
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 1 cut(s) 269
MalI GATC 4 cut(s) 62, 369, 402, 511
MbiI CCGCTC 1 cut(s) 78
MboI GATC 4 cut(s) 60, 367, 400, 509
MboII GAAGA 3 cut(s) 542, 566, 796
MhlI GDGCHC 1 cut(s) 671
MlsI TGGCCA 1 cut(s) 307
MluCI AATT 5 cut(s) 21, 82, 185, 239, 297
MluNI TGGCCA 1 cut(s) 307
MmeI TCCRAC 2 cut(s) 679, 703
MnlI CCTC 7 cut(s) 81, 165, 174, 250, 276, 520, 580
Mox20I TGGCCA 1 cut(s) 307
MroXI GAANNNNTTC 4 cut(s) 298, 470, 696, 788
MscI TGGCCA 1 cut(s) 307
MseI TTAA 2 cut(s) 242, 482
MslI CAYNNNNRTG 2 cut(s) 313, 636
Msp20I TGGCCA 1 cut(s) 307
MspA1I CMGCKG 2 cut(s) 406, 719
MspI CCGG 2 cut(s) 169, 418
MspR9I CCNGG 2 cut(s) 291, 841
MvaI CCWGG 2 cut(s) 291, 841
MvnI CGCG 4 cut(s) 14, 149, 508, 813
MwoI GCNNNNNNNGC 5 cut(s) 97, 106, 126, 169, 257
NdeII GATC 4 cut(s) 60, 367, 400, 509
NlaIII CATG 6 cut(s) 223, 277, 306, 314, 425, 445
NlaIV GGNNCC 3 cut(s) 162, 288, 473
NmuCI GTSAC 1 cut(s) 269
NruI TCGCGA 1 cut(s) 508
NspI RCATGY 3 cut(s) 277, 314, 425
NspV TTCGAA 1 cut(s) 804
OliI CACNNNNGTG 1 cut(s) 313
PauI GCGCGC 1 cut(s) 12
PciI ACATGT 1 cut(s) 310
PcsI WCGNNNNNNNCGW 1 cut(s) 810
PdmI GAANNNNTTC 4 cut(s) 298, 470, 696, 788
PfeI GAWTC 2 cut(s) 599, 616
PkrI GCNGC 3 cut(s) 77, 108, 408
Ple19I CGATCG 1 cut(s) 63
PscI ACATGT 1 cut(s) 310
Psp6I CCWGG 2 cut(s) 289, 839
PspGI CCWGG 2 cut(s) 289, 839
PspN4I GGNNCC 3 cut(s) 162, 288, 473
PspPI GGNCC 1 cut(s) 287
PteI GCGCGC 1 cut(s) 12
PvuI CGATCG 1 cut(s) 63
PvuII CAGCTG 2 cut(s) 406, 719
RruI TCGCGA 1 cut(s) 508
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
RseI CAYNNNNRTG 2 cut(s) 313, 636
SaqAI TTAA 2 cut(s) 242, 482
SatI GCNGC 3 cut(s) 76, 107, 407
Sau3AI GATC 4 cut(s) 60, 367, 400, 509
Sau96I GGNCC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 291, 841
SduI GDGCHC 1 cut(s) 671
SetI ASST 8 cut(s) 137, 354, 408, 521, 573, 591, 721, 842
SfaNI GCATC 1 cut(s) 396
SfcI CTRYAG 1 cut(s) 436
SfuI TTCGAA 1 cut(s) 804
SinI GGWCC 1 cut(s) 287
SmiMI CAYNNNNRTG 2 cut(s) 313, 636
Sse9I AATT 5 cut(s) 21, 82, 185, 239, 297
SsiI CCGC 4 cut(s) 76, 127, 147, 629
StyD4I CCNGG 2 cut(s) 289, 839
TaaI ACNGT 3 cut(s) 398, 714, 822
TaiI ACGT 1 cut(s) 573
TaqI TCGA 6 cut(s) 59, 63, 114, 204, 255, 804
TasI AATT 5 cut(s) 21, 82, 185, 239, 297
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 2 cut(s) 599, 616
Tru1I TTAA 2 cut(s) 242, 482
Tru9I TTAA 2 cut(s) 242, 482
TscAI CASTG 2 cut(s) 678, 766
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 2 cut(s) 106, 406
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 2 cut(s) 291, 657
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 2 cut(s) 678, 766
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 1 cut(s) 297
XceI RCATGY 3 cut(s) 277, 314, 425
XmnI GAANNNNTTC 4 cut(s) 298, 470, 696, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.