pycom15g13270

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007953
Physical Location & Seq
Forward (+)
9087641 .. 9090853
3213 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
ATGACAAACGACTTTAAGCAGATAATGTGTAAAATATCAAACAAGCACACCAACCGAACGAACCACACAGGGCGGAAGAAGAAGATGATGCCCAGATGCTCTGTTCAATCGACCGACGCTCAGCTCGTCCAGAGCATAAATGGCCTCCAACAGCTTGCCGAAACGCACCGTTTCAAGGCCTGGTTCTTGGATCAGTTTGGAGTTCTCCATGATGGGAAGCAACCTTACCCTGGTGCAATTTCAACATTGAAGAAGCTAGCAAGCAGTGGTGCAAAGATGGTTATTATAAGTAATTCATCAAGACGCTCGTCTACAACCATGGATAAATTGAAAAGCCTTGGGTTCGATCCTTCTCTTTTTGTAGGAGCCATCACTAGTGGGGAACTGACACATCAAAACTTACTAAGGAGAGATGATGCTTGGTTCGCTGCACTGGGAAAATCTTGTATTCATATGACTTGGAGTGCTCGTGGTACCGTATCTCTTGAGGGATTGGGATTACAAGTTGTGGAGAATGTTCAAGACGCCGAATTTATTTTGGCCCATGGCTCTGAAGCCTTGGGGAGTCCTTCTGGTGATGCGATTCCAATGAAACTTGAGGAACTTGAAAGTATATTGGAACAATGTGCTGCTAAACACATCCCTATGGTGGTAGCAAATCCAGATTTTGTGACTGTTGAGGCTAGAGCTTTGCGTGTAATGCCTGGGACTTTGGCTTCCAAGTATGAAAAGCTCGGGGGTGAAGTGAAATGGATGGGCAAGCCTGATAAGATCATCTACAAATCAGCTATGGACTTAGCTGGTGTAGAGCCTGCTGAATCTATCGCAGTTGGCGATTCTCTTCACCACGACATCAAGGGTGCAAATGCAGCTGCAATCCAATCAGTTTTTGTCACCTGTGGGATCCATGGAAATGAACTTGGACTCAATAGTTTCGCGGACGTTGCAGATTTATCTTCCGTGCAAGCTCTTGCTTCCAAATACGATGCATACCCGTCGTACGTGTTACCTGCATTCACATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_6 Hydrolase_like

Protein Analysis

342

Amino Acids

36.96

Weight (kDa)

7.7

Isoelectric Point (pI)

32.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 61 - 137 3.6e-14 Haloacid dehalogenase-like hydrolase
Hydrolase PF00702 201 - 291 4e-07 haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 252 - 306 1.4e-12 HAD-hyrolase-like
HAD_2 PF13419 253 - 298 4.6e-06 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 1018
Acc65I GGTACC 1 cut(s) 473
AccB1I GGYRCC 1 cut(s) 473
AccI GTMKAC 1 cut(s) 311
AccII CGCG 1 cut(s) 938
AciI CCGC 2 cut(s) 73, 938
AclWI GGATC 4 cut(s) 198, 341, 898, 911
AcsI RAATTY 1 cut(s) 530
AcuI CTGAAG 1 cut(s) 573
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 2 cut(s) 475, 1001
AfiI CCNNNNNNNGG 3 cut(s) 175, 230, 649
AflIII ACRYGT 1 cut(s) 1002
AgsI TTSAA 7 cut(s) 107, 175, 243, 250, 331, 521, 608
AhlI ACTAGT 1 cut(s) 374
AjnI CCWGG 3 cut(s) 179, 229, 703
AluBI AGCT 9 cut(s) 124, 154, 256, 689, 733, 788, 800, 872, 968
AluI AGCT 9 cut(s) 124, 154, 256, 689, 733, 788, 800, 872, 968
Alw21I GWGCWC 1 cut(s) 469
AlwI GGATC 4 cut(s) 198, 341, 898, 911
Ama87I CYCGRG 1 cut(s) 734
AoxI GGCC 3 cut(s) 142, 177, 540
ApeKI GCWGC 4 cut(s) 428, 629, 869, 872
ApoI RAATTY 1 cut(s) 530
Asp718I GGTACC 1 cut(s) 473
AspS9I GGNCC 1 cut(s) 541
AsuHPI GGTGA 4 cut(s) 587, 752, 836, 886
AsuNHI GCTAGC 1 cut(s) 256
AvaI CYCGRG 1 cut(s) 734
BamHI GGATCC 1 cut(s) 903
BanI GGYRCC 1 cut(s) 473
BarI GAAGNNNNNNTAC 2 cut(s) 209, 241
BauI CACGAG 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 4 cut(s) 415, 616, 859, 881
BccI CCATC 4 cut(s) 206, 271, 377, 748
BcgI CGANNNNNNTGC 2 cut(s) 978, 1012
BciT130I CCWGG 3 cut(s) 181, 231, 705
BcuI ACTAGT 1 cut(s) 374
BfaI CTAG 3 cut(s) 257, 375, 684
BfuAI ACCTGC 1 cut(s) 1018
BisI GCNGC 4 cut(s) 429, 630, 870, 873
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 4 cut(s) 430, 631, 871, 874
Bme1390I CCNGG 3 cut(s) 181, 231, 705
BmeT110I CYCGRG 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 541
BmiI GGNNCC 3 cut(s) 367, 475, 905
BmrFI CCNGG 3 cut(s) 181, 231, 705
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 5 cut(s) 78, 86, 406, 568, 976
BmtI GCTAGC 1 cut(s) 260
BmuI ACTGGG 1 cut(s) 443
BoxI GACNNNNGTC 1 cut(s) 307
Bpu1102I GCTNAGC 1 cut(s) 120
BpuEI CTTGAG 2 cut(s) 506, 617
BsaAI YACGTR 1 cut(s) 1003
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 7 cut(s) 229, 318, 337, 544, 558, 704, 907
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 230, 649
Bse1I ACTGG 1 cut(s) 438
BseBI CCWGG 3 cut(s) 181, 231, 705
BseDI CCNNGG 7 cut(s) 229, 318, 337, 544, 558, 704, 907
BseGI GGATG 2 cut(s) 639, 759
BseLI CCNNNNNNNGG 3 cut(s) 175, 230, 649
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 438
BseXI GCAGC 4 cut(s) 415, 616, 859, 881
BsgI GTGCAG 1 cut(s) 414
Bsh1236I CGCG 1 cut(s) 938
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 3 cut(s) 144, 179, 542
BshNI GGYRCC 1 cut(s) 473
BsiEI CGRYCG 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 469
BsiHKCI CYCGRG 1 cut(s) 734
BsiWI CGTACG 1 cut(s) 999
BslFI GGGAC 1 cut(s) 721
BslI CCNNNNNNNGG 3 cut(s) 175, 230, 649
BsmFI GGGAC 1 cut(s) 721
BsmI GAATGC 1 cut(s) 1013
BsnI GGCC 3 cut(s) 144, 179, 542
BsoBI CYCGRG 1 cut(s) 734
Bsp1286I GDGCHC 1 cut(s) 469
Bsp143I GATC 4 cut(s) 190, 346, 771, 903
Bsp1720I GCTNAGC 1 cut(s) 120
Bsp19I CCATGG 3 cut(s) 318, 544, 907
BspACI CCGC 2 cut(s) 73, 938
BspANI GGCC 3 cut(s) 144, 179, 542
BspCNI CTCAG 1 cut(s) 133
BspFNI CGCG 1 cut(s) 938
BspLI GGNNCC 3 cut(s) 367, 475, 905
BspMI ACCTGC 1 cut(s) 1018
BspOI GCTAGC 1 cut(s) 260
BspPI GGATC 4 cut(s) 198, 341, 898, 911
BspT107I GGYRCC 1 cut(s) 473
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 7 cut(s) 229, 318, 337, 544, 558, 704, 907
BssMI GATC 4 cut(s) 190, 346, 771, 903
BssNI GRCGYC 1 cut(s) 525
BssSI CACGAG 1 cut(s) 468
BssT1I CCWWGG 5 cut(s) 318, 337, 544, 558, 907
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 3 cut(s) 181, 231, 705
Bst4CI ACNGT 3 cut(s) 170, 478, 676
Bst6I CTCTTC 1 cut(s) 846
BstACI GRCGYC 1 cut(s) 525
BstBAI YACGTR 1 cut(s) 1003
BstC8I GCNNGC 6 cut(s) 156, 258, 262, 761, 813, 966
BstDEI CTNAG 3 cut(s) 120, 404, 796
BstDSI CCRYGG 3 cut(s) 318, 544, 907
BstF5I GGATG 2 cut(s) 639, 759
BstFNI CGCG 1 cut(s) 938
BstKTI GATC 4 cut(s) 193, 349, 774, 906
BstMBI GATC 4 cut(s) 190, 346, 771, 903
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 5 cut(s) 141, 425, 700, 869, 944
BstNI CCWGG 3 cut(s) 181, 231, 705
BstPAI GACNNNNGTC 1 cut(s) 307
BstSCI CCNGG 3 cut(s) 179, 229, 703
BstUI CGCG 1 cut(s) 938
BstV1I GCAGC 4 cut(s) 415, 616, 859, 881
BstX2I RGATCY 1 cut(s) 903
BstYI RGATCY 1 cut(s) 903
BsuRI GGCC 3 cut(s) 144, 179, 542
BtgI CCRYGG 3 cut(s) 318, 544, 907
BtsCI GGATG 2 cut(s) 639, 759
BtsI GCAGTG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 271, 431
BveI ACCTGC 1 cut(s) 1018
Cac8I GCNNGC 6 cut(s) 156, 258, 262, 761, 813, 966
Cfr13I GGNCC 1 cut(s) 541
CseI GACGC 3 cut(s) 125, 312, 533
Csp6I GTAC 2 cut(s) 474, 1000
CviAII CATG 5 cut(s) 209, 319, 545, 908, 1020
CviQI GTAC 2 cut(s) 474, 1000
DdeI CTNAG 3 cut(s) 120, 404, 796
DpnI GATC 4 cut(s) 192, 348, 773, 905
DpnII GATC 4 cut(s) 190, 346, 771, 903
Eam1104I CTCTTC 1 cut(s) 846
EarI CTCTTC 1 cut(s) 846
EciI GGCGGA 1 cut(s) 88
Eco130I CCWWGG 5 cut(s) 318, 337, 544, 558, 907
Eco147I AGGCCT 1 cut(s) 179
Eco57I CTGAAG 1 cut(s) 573
Eco88I CYCGRG 1 cut(s) 734
EcoRII CCWGG 3 cut(s) 179, 229, 703
EcoT14I CCWWGG 5 cut(s) 318, 337, 544, 558, 907
EcoT22I ATGCAT 1 cut(s) 991
ErhI CCWWGG 5 cut(s) 318, 337, 544, 558, 907
FaeI CATG 5 cut(s) 212, 322, 548, 911, 1023
FaqI GGGAC 1 cut(s) 721
FatI CATG 5 cut(s) 208, 318, 544, 907, 1019
FauNDI CATATG 1 cut(s) 453
FblI GTMKAC 1 cut(s) 311
Fnu4HI GCNGC 4 cut(s) 429, 630, 870, 873
FokI GGATG 2 cut(s) 626, 766
Fsp4HI GCNGC 4 cut(s) 429, 630, 870, 873
FspBI CTAG 3 cut(s) 257, 375, 684
GluI GCNGC 4 cut(s) 429, 630, 870, 873
HaeIII GGCC 3 cut(s) 144, 179, 542
HgaI GACGC 3 cut(s) 125, 312, 533
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 5 cut(s) 212, 322, 548, 911, 1023
HinfI GANTC 5 cut(s) 565, 583, 818, 836, 924
HphI GGTGA 4 cut(s) 587, 752, 836, 886
Hpy166II GTNNAC 1 cut(s) 312
Hpy188I TCNGA 1 cut(s) 553
Hpy188III TCNNGA 5 cut(s) 130, 300, 485, 521, 662
Hpy8I GTNNAC 1 cut(s) 312
Hpy99I CGWCG 2 cut(s) 119, 1000
HpyAV CCTTC 2 cut(s) 360, 579
HpyCH4III ACNGT 3 cut(s) 170, 478, 676
HpyCH4IV ACGT 2 cut(s) 942, 1002
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 425, 700, 869, 944
HpyF3I CTNAG 3 cut(s) 120, 404, 796
HpySE526I ACGT 2 cut(s) 942, 1002
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 5 cut(s) 212, 322, 548, 911, 1023
KpnI GGTACC 1 cut(s) 477
Kzo9I GATC 4 cut(s) 190, 346, 771, 903
LmnI GCTCC 1 cut(s) 365
Lsp1109I GCAGC 4 cut(s) 415, 616, 859, 881
LweI GCATC 5 cut(s) 78, 86, 406, 568, 976
MaeI CTAG 3 cut(s) 257, 375, 684
MaeII ACGT 2 cut(s) 942, 1002
MaeIII GTNAC 3 cut(s) 670, 892, 1005
MalI GATC 4 cut(s) 192, 348, 773, 905
MboI GATC 4 cut(s) 190, 346, 771, 903
MboII GAAGA 6 cut(s) 88, 91, 94, 262, 833, 948
MflI RGATCY 1 cut(s) 903
MhlI GDGCHC 1 cut(s) 469
MluCI AATT 4 cut(s) 237, 292, 326, 530
MlyI GAGTC 2 cut(s) 574, 918
MmeI TCCRAC 1 cut(s) 172
MnlI CCTC 4 cut(s) 155, 481, 592, 673
Mph1103I ATGCAT 1 cut(s) 991
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 3 cut(s) 644, 912, 1018
MspA1I CMGCKG 1 cut(s) 872
MspR9I CCNGG 3 cut(s) 181, 231, 705
Mva1269I GAATGC 1 cut(s) 1013
MvaI CCWGG 3 cut(s) 181, 231, 705
MvnI CGCG 1 cut(s) 938
MwoI GCNNNNNNNGC 5 cut(s) 141, 425, 700, 869, 944
NcoI CCATGG 3 cut(s) 318, 544, 907
NdeI CATATG 1 cut(s) 453
NdeII GATC 4 cut(s) 190, 346, 771, 903
NheI GCTAGC 1 cut(s) 256
NlaIII CATG 5 cut(s) 212, 322, 548, 911, 1023
NlaIV GGNNCC 3 cut(s) 367, 475, 905
NmuCI GTSAC 2 cut(s) 670, 892
NsiI ATGCAT 1 cut(s) 991
PceI AGGCCT 1 cut(s) 179
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 831
PctI GAATGC 1 cut(s) 1013
PfeI GAWTC 3 cut(s) 583, 818, 836
Pfl23II CGTACG 1 cut(s) 999
PkrI GCNGC 4 cut(s) 430, 631, 871, 874
PleI GAGTC 2 cut(s) 573, 918
PpsI GAGTC 2 cut(s) 573, 918
Ppu21I YACGTR 1 cut(s) 1003
PshAI GACNNNNGTC 1 cut(s) 307
PsiI TTATAA 1 cut(s) 287
Psp6I CCWGG 3 cut(s) 179, 229, 703
PspGI CCWGG 3 cut(s) 179, 229, 703
PspLI CGTACG 1 cut(s) 999
PspN4I GGNNCC 3 cut(s) 367, 475, 905
PspPI GGNCC 1 cut(s) 541
PsuI RGATCY 1 cut(s) 903
PvuII CAGCTG 1 cut(s) 872
RsaI GTAC 2 cut(s) 475, 1001
RsaNI GTAC 2 cut(s) 474, 1000
RseI CAYNNNNRTG 3 cut(s) 644, 912, 1018
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 4 cut(s) 429, 630, 870, 873
Sau3AI GATC 4 cut(s) 190, 346, 771, 903
Sau96I GGNCC 1 cut(s) 541
SchI GAGTC 2 cut(s) 574, 918
ScrFI CCNGG 3 cut(s) 181, 231, 705
SduI GDGCHC 1 cut(s) 469
SfaNI GCATC 5 cut(s) 78, 86, 406, 568, 976
SmiMI CAYNNNNRTG 3 cut(s) 644, 912, 1018
SmlI CTYRAG 2 cut(s) 485, 596
SmoI CTYRAG 2 cut(s) 485, 596
SpeI ACTAGT 1 cut(s) 374
Sse9I AATT 4 cut(s) 237, 292, 326, 530
SseBI AGGCCT 1 cut(s) 179
SsiI CCGC 2 cut(s) 73, 938
SspMI CTAG 3 cut(s) 257, 375, 684
StuI AGGCCT 1 cut(s) 179
StyD4I CCNGG 3 cut(s) 179, 229, 703
StyI CCWWGG 5 cut(s) 318, 337, 544, 558, 907
TaaI ACNGT 3 cut(s) 170, 478, 676
TaiI ACGT 2 cut(s) 945, 1005
TaqI TCGA 2 cut(s) 110, 345
TaqII GACCGA 1 cut(s) 128
TasI AATT 4 cut(s) 237, 292, 326, 530
TfiI GAWTC 3 cut(s) 583, 818, 836
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 2 cut(s) 271, 438
TseFI GTSAC 2 cut(s) 670, 892
TseI GCWGC 4 cut(s) 428, 629, 869, 872
Tsp45I GTSAC 2 cut(s) 670, 892
TspDTI ATGAA 5 cut(s) 285, 440, 605, 741, 930
TspGWI ACGGA 1 cut(s) 949
TspRI CASTG 2 cut(s) 271, 438
XapI RAATTY 1 cut(s) 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 311
XspI CTAG 3 cut(s) 257, 375, 684
Zsp2I ATGCAT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.