pycom15g17980

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006686
Physical Location & Seq
Forward (+)
12762735 .. 12765031
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
ATGGGCGACGAGGTCGATACTATTTTATCTTACTGCTTTCAGAAACAGCCCAAACAGCCCCTCACTTTGCATTGCTTCAGTCTCGTAGTCTTTCACTCTTCAGCTCCGTTCAGCTTACAGAGGCGCACGTTAACCATGCTTAATCTACACACCCGCTCACCTGTTCTTCAACCTCCCTGCACTCCCTTATCCATGTTTTCCCGCAACACAAACCGCTTCCCGCCATTTAGTACCGTTGGACTTCACTTTCAGGGCTTTTCTGCACAGCCGGGGGAGCACGGGACCTCCGTATTCGTTGCAAAAGCGGGTCGGGGCCTAAGGCTGAAGACCCAGAAAGCGAGTGACGGAGAAGAAGATAAAGACGGCGGCTTTGTGGATATAGATTTCGAAGTTGCAGCTGAATCCGAAGACGGGTTTCCGGGTCGGACCGGCTTCGGAGGGAGAGAGGAAGAAAAGGACTTTGATCGGGATCCCGAGTTTGCTGAAATTATGGGTGCTTGTCTTGATGACCCAGAAAAAGCTAGGTCCAAGATGGAAGATAGGTTGAGGAAGAAAAGGAACAAGATTTTGCAGACAAAGTCTGGTTCAGGGCTGCCCATGAATGTGACATTCAACAACTTTGGTTTCTCAAACTCGTATATATGGTTTGAATTCTACAACACTCCATTGGAGCAAGATGTTACCTTAATCTCTGATACCATTCGATCATGGCACATCATTGGGCGCATGGGTGGATGCAATTCCATGAATATGCAGTTGTCACAGTCTCCTTTCGATAAACGACCAAGTTATGATGCAATTCAGGGAGCAAATGTGACCCCAACTACTTTTTACAATATCGGAGATCTTGAGGTTCAGGACAACTTAGCACGTGTTTGGGTGGATATCGGCACCAGTGAACCATTGCTACTGGATGTTCTAATAAACGCATTGACTCAGATAAGCTCCGACTTTGTCGGAATCAAGCAAGTGGTGTTTGGTGGATCTGAATTTGAAAACTGGAAGGAGAACATGACATCAGAGGATGCAGGTTATAGCATCCACAACATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

351

Amino Acids

39.19

Weight (kDa)

5.11

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 200 - 341 4e-53 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1019
AccB1I GGYRCC 1 cut(s) 890
AccBSI CCGCTC 1 cut(s) 156
AciI CCGC 6 cut(s) 154, 202, 214, 221, 305, 366
AclWI GGATC 3 cut(s) 464, 477, 991
AcsI RAATTY 2 cut(s) 650, 989
AcuI CTGAAG 3 cut(s) 61, 84, 344
AcvI CACGTG 1 cut(s) 872
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 1 cut(s) 411
AflIII ACRYGT 1 cut(s) 871
AgsI TTSAA 4 cut(s) 170, 613, 650, 995
AluBI AGCT 5 cut(s) 104, 114, 398, 521, 945
AluI AGCT 5 cut(s) 104, 114, 398, 521, 945
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 2 cut(s) 86, 771
AlwI GGATC 3 cut(s) 464, 477, 991
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 1 cut(s) 313
ApeKI GCWGC 2 cut(s) 395, 592
ApoI RAATTY 2 cut(s) 650, 989
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AspLEI GCGC 2 cut(s) 126, 726
AspS9I GGNCC 4 cut(s) 282, 313, 426, 525
AsuC2I CCSGG 2 cut(s) 270, 420
AsuHPI GGTGA 1 cut(s) 150
AsuII TTCGAA 1 cut(s) 387
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 3 cut(s) 282, 426, 525
AxyI CCTNAGG 1 cut(s) 317
BamHI GGATCC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 890
BbrPI CACGTG 1 cut(s) 872
BbsI GAAGAC 2 cut(s) 332, 414
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 407, 579
BccI CCATC 1 cut(s) 526
BceAI ACGGC 1 cut(s) 379
BcnI CCSGG 2 cut(s) 270, 420
BcoDI GTCTC 2 cut(s) 86, 771
BfaI CTAG 1 cut(s) 522
BfuAI ACCTGC 1 cut(s) 1019
BglII AGATCT 1 cut(s) 844
BisI GCNGC 3 cut(s) 367, 396, 593
BlsI GCNGC 3 cut(s) 368, 397, 594
Bme1390I CCNGG 2 cut(s) 270, 420
Bme18I GGWCC 3 cut(s) 282, 426, 525
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 4 cut(s) 282, 313, 426, 525
BmiI GGNNCC 4 cut(s) 283, 314, 471, 892
BmrFI CCNGG 2 cut(s) 270, 420
BmsI GCATC 4 cut(s) 725, 784, 1015, 1047
BpiI GAAGAC 2 cut(s) 332, 414
Bpu14I TTCGAA 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 869
BpuMI CCSGG 2 cut(s) 270, 420
BsaAI YACGTR 1 cut(s) 872
BsaBI GATNNNNATC 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 269
BsaXI ACNNNNNCTCC 4 cut(s) 269, 299, 798, 828
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse118I RCCGGY 1 cut(s) 428
Bse1I ACTGG 3 cut(s) 894, 915, 1004
Bse21I CCTNAGG 1 cut(s) 317
Bse3DI GCAATG 2 cut(s) 70, 902
Bse8I GATNNNNATC 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 269
BseGI GGATG 4 cut(s) 740, 919, 1030, 1038
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 1 cut(s) 411
BseMI GCAATG 2 cut(s) 70, 902
BseMII CTCAG 1 cut(s) 950
BseNI ACTGG 3 cut(s) 894, 915, 1004
BseXI GCAGC 2 cut(s) 407, 579
BsgI GTGCAG 2 cut(s) 163, 246
BshFI GGCC 1 cut(s) 315
BshNI GGYRCC 1 cut(s) 890
BsiHKAI GWGCWC 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 3 cut(s) 269, 419, 429
BslFI GGGAC 1 cut(s) 295
BslI CCNNNNNNNGG 1 cut(s) 411
BsmAI GTCTC 2 cut(s) 86, 771
BsmFI GGGAC 1 cut(s) 295
BsnI GGCC 1 cut(s) 315
BsoBI CYCGRG 1 cut(s) 473
Bsp119I TTCGAA 1 cut(s) 387
Bsp1286I GDGCHC 1 cut(s) 279
Bsp143I GATC 5 cut(s) 463, 469, 704, 844, 983
BspACI CCGC 6 cut(s) 154, 202, 214, 221, 305, 366
BspANI GGCC 1 cut(s) 315
BspCNI CTCAG 1 cut(s) 949
BspLI GGNNCC 4 cut(s) 283, 314, 471, 892
BspMI ACCTGC 1 cut(s) 1019
BspPI GGATC 3 cut(s) 464, 477, 991
BspT104I TTCGAA 1 cut(s) 387
BspT107I GGYRCC 1 cut(s) 890
BsrBI CCGCTC 1 cut(s) 156
BsrDI GCAATG 2 cut(s) 70, 902
BsrFI RCCGGY 1 cut(s) 428
BsrI ACTGG 3 cut(s) 894, 915, 1004
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 1 cut(s) 269
BssMI GATC 5 cut(s) 463, 469, 704, 844, 983
Bst4CI ACNGT 2 cut(s) 235, 765
Bst6I CTCTTC 1 cut(s) 103
BstBAI YACGTR 1 cut(s) 872
BstBI TTCGAA 1 cut(s) 387
BstDEI CTNAG 3 cut(s) 317, 865, 936
BstF5I GGATG 4 cut(s) 740, 919, 1030, 1038
BstHHI GCGC 2 cut(s) 126, 726
BstKTI GATC 5 cut(s) 466, 472, 707, 847, 986
BstMAI GTCTC 2 cut(s) 86, 771
BstMBI GATC 5 cut(s) 463, 469, 704, 844, 983
BstMWI GCNNNNNNNGC 2 cut(s) 55, 274
BstSCI CCNGG 2 cut(s) 268, 418
BstV1I GCAGC 2 cut(s) 407, 579
BstV2I GAAGAC 2 cut(s) 332, 414
BstX2I RGATCY 3 cut(s) 469, 844, 983
BstYI RGATCY 3 cut(s) 469, 844, 983
Bsu36I CCTNAGG 1 cut(s) 317
BsuRI GGCC 1 cut(s) 315
BtsCI GGATG 4 cut(s) 740, 919, 1030, 1038
BtsIMutI CAGTG 1 cut(s) 901
BveI ACCTGC 1 cut(s) 1019
CfoI GCGC 2 cut(s) 126, 726
Cfr10I RCCGGY 1 cut(s) 428
Cfr13I GGNCC 4 cut(s) 282, 313, 426, 525
CpoI CGGWCCG 1 cut(s) 426
Csp6I GTAC 1 cut(s) 231
CspI CGGWCCG 1 cut(s) 426
CviAII CATG 7 cut(s) 136, 193, 598, 708, 727, 745, 1012
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 3 cut(s) 317, 865, 936
DpnI GATC 5 cut(s) 465, 471, 706, 846, 985
DpnII GATC 5 cut(s) 463, 469, 704, 844, 983
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 3 cut(s) 282, 426, 525
Eco57I CTGAAG 3 cut(s) 61, 84, 344
Eco72I CACGTG 1 cut(s) 872
Eco81I CCTNAGG 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 473
EcoO109I RGGNCCY 2 cut(s) 282, 313
EcoRI GAATTC 1 cut(s) 650
EcoRV GATATC 1 cut(s) 886
FaeI CATG 7 cut(s) 139, 196, 601, 711, 730, 748, 1015
FaqI GGGAC 1 cut(s) 295
FatI CATG 7 cut(s) 135, 192, 597, 707, 726, 744, 1011
FauI CCCGC 4 cut(s) 161, 209, 228, 298
Fnu4HI GCNGC 3 cut(s) 367, 396, 593
FokI GGATG 4 cut(s) 747, 926, 1025, 1037
Fsp4HI GCNGC 3 cut(s) 367, 396, 593
FspBI CTAG 1 cut(s) 522
GlaI GCGC 2 cut(s) 125, 725
GluI GCNGC 3 cut(s) 367, 396, 593
HaeIII GGCC 1 cut(s) 315
HapII CCGG 3 cut(s) 269, 419, 429
HhaI GCGC 2 cut(s) 126, 726
Hin1II CATG 7 cut(s) 139, 196, 601, 711, 730, 748, 1015
Hin6I GCGC 2 cut(s) 124, 724
HinP1I GCGC 2 cut(s) 124, 724
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HinfI GANTC 3 cut(s) 401, 934, 960
HpaI GTTAAC 1 cut(s) 132
HpaII CCGG 3 cut(s) 269, 419, 429
HphI GGTGA 1 cut(s) 150
Hpy166II GTNNAC 2 cut(s) 132, 899
Hpy188III TCNNGA 5 cut(s) 467, 473, 503, 848, 857
Hpy8I GTNNAC 2 cut(s) 132, 899
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 1 cut(s) 997
HpyCH4III ACNGT 2 cut(s) 235, 765
HpyCH4IV ACGT 2 cut(s) 128, 871
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 274
HpyF3I CTNAG 3 cut(s) 317, 865, 936
HpySE526I ACGT 2 cut(s) 128, 871
Hsp92II CATG 7 cut(s) 139, 196, 601, 711, 730, 748, 1015
HspAI GCGC 2 cut(s) 124, 724
KspAI GTTAAC 1 cut(s) 132
Kzo9I GATC 5 cut(s) 463, 469, 704, 844, 983
LmnI GCTCC 5 cut(s) 109, 274, 670, 806, 950
Lsp1109I GCAGC 2 cut(s) 407, 579
LweI GCATC 4 cut(s) 725, 784, 1015, 1047
MaeI CTAG 1 cut(s) 522
MaeII ACGT 2 cut(s) 128, 871
MaeIII GTNAC 5 cut(s) 341, 604, 679, 759, 814
MalI GATC 5 cut(s) 465, 471, 706, 846, 985
MbiI CCGCTC 1 cut(s) 156
MboI GATC 5 cut(s) 463, 469, 704, 844, 983
MboII GAAGA 9 cut(s) 90, 158, 337, 362, 365, 419, 461, 548, 562
MflI RGATCY 3 cut(s) 469, 844, 983
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 5 cut(s) 486, 650, 739, 798, 989
MlyI GAGTC 1 cut(s) 928
MmeI TCCRAC 4 cut(s) 217, 404, 937, 972
MseI TTAA 3 cut(s) 131, 141, 686
MslI CAYNNNNRTG 2 cut(s) 602, 749
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 3 cut(s) 269, 419, 429
MspR9I CCNGG 2 cut(s) 270, 420
MwoI GCNNNNNNNGC 2 cut(s) 55, 274
NciI CCSGG 2 cut(s) 270, 420
NdeII GATC 5 cut(s) 463, 469, 704, 844, 983
NlaIII CATG 7 cut(s) 139, 196, 601, 711, 730, 748, 1015
NlaIV GGNNCC 4 cut(s) 283, 314, 471, 892
NmuCI GTSAC 4 cut(s) 341, 604, 759, 814
NspV TTCGAA 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PfeI GAWTC 2 cut(s) 401, 960
PflFI GACNNNGTC 3 cut(s) 11, 577, 953
PkrI GCNGC 3 cut(s) 368, 397, 594
PleI GAGTC 1 cut(s) 928
PmaCI CACGTG 1 cut(s) 872
PmlI CACGTG 1 cut(s) 872
PpsI GAGTC 1 cut(s) 928
Ppu21I YACGTR 1 cut(s) 872
PpuMI RGGWCCY 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 282
PspCI CACGTG 1 cut(s) 872
PspN4I GGNNCC 4 cut(s) 283, 314, 471, 892
PspPI GGNCC 4 cut(s) 282, 313, 426, 525
PspPPI RGGWCCY 1 cut(s) 282
PsrI GAACNNNNNNTAC 2 cut(s) 891, 923
PsuI RGATCY 3 cut(s) 469, 844, 983
PsyI GACNNNGTC 3 cut(s) 11, 577, 953
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
RseI CAYNNNNRTG 2 cut(s) 602, 749
Rsr2I CGGWCCG 1 cut(s) 426
RsrII CGGWCCG 1 cut(s) 426
SaqAI TTAA 3 cut(s) 131, 141, 686
SatI GCNGC 3 cut(s) 367, 396, 593
Sau3AI GATC 5 cut(s) 463, 469, 704, 844, 983
Sau96I GGNCC 4 cut(s) 282, 313, 426, 525
SchI GAGTC 1 cut(s) 928
ScrFI CCNGG 2 cut(s) 270, 420
SduI GDGCHC 1 cut(s) 279
SfaNI GCATC 4 cut(s) 725, 784, 1015, 1047
SfuI TTCGAA 1 cut(s) 387
SinI GGWCC 3 cut(s) 282, 426, 525
SmiMI CAYNNNNRTG 2 cut(s) 602, 749
SmlI CTYRAG 1 cut(s) 848
SmoI CTYRAG 1 cut(s) 848
Sse9I AATT 5 cut(s) 486, 650, 739, 798, 989
SsiI CCGC 6 cut(s) 154, 202, 214, 221, 305, 366
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 2 cut(s) 268, 418
TaaI ACNGT 2 cut(s) 235, 765
TaiI ACGT 2 cut(s) 131, 874
TaqI TCGA 4 cut(s) 15, 387, 703, 774
TasI AATT 5 cut(s) 486, 650, 739, 798, 989
TauI GCSGC 1 cut(s) 369
TfiI GAWTC 2 cut(s) 401, 960
Tru1I TTAA 3 cut(s) 131, 141, 686
Tru9I TTAA 3 cut(s) 131, 141, 686
TscAI CASTG 1 cut(s) 901
TseFI GTSAC 4 cut(s) 341, 604, 759, 814
TseI GCWGC 2 cut(s) 395, 592
Tsp45I GTSAC 4 cut(s) 341, 604, 759, 814
TspDTI ATGAA 2 cut(s) 614, 761
TspGWI ACGGA 3 cut(s) 96, 277, 360
TspRI CASTG 1 cut(s) 901
Tth111I GACNNNGTC 3 cut(s) 11, 577, 953
VpaK11BI GGWCC 3 cut(s) 282, 426, 525
XapI RAATTY 2 cut(s) 650, 989
XspI CTAG 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.