pycom15g21350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006081
Physical Location & Seq
Forward (+)
15343535 .. 15346384
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 822 bp
ATGTCTCGGTGTTTTCCGTACCCTCCTCCTGGGTACGTGAAGAAGGGGATCCGCGATGAGGCCCTGATCGATTCGATTAAGCTCCAAAGAGAAGATGAGAAGGCCAAGAAAGAAAAGAAGAGGGAGAAAAAACGAGAGAAGAAAGAAAAGAAGGCTCGAGAAAATGGGGAGCCTGAAAATAAGAAGCAGAGTCATAAAAAAAGGCACAAAGATGAAAGGTACCAAGAAGACGAGAAAGGTACAGACCATGGAAAGAAGAGAAAACATGAAACGGAAAACCTTGACAAGAGTGGCCTTACTGAAGAACACGAGCAACCAGTTGGTTCCCAGAACTCTTCTGATAGCACCGTTAACAGCAACAAAAGGCAGAAGCAGGACTCTCCCCCTGATGGCAGGCATAATTCTGCAAGCATTCTTCGGATCCGGTTGCCTCTTCAAAGGCACAAAGATCCTGAAATGCTACCCAGAGAGGAGCAGCCTTGCCAGTTGCCTTTAGAAACGCACAAAGATCCAGTGATGCTATCCAGAGAGAAACAACCTAGCCAGTTGCCTCTCCCAAAGCACAAGGATCCAGTAGTGCTATCAAAAGAGGAACAGCCTTACCGGTTGTCACTTCAAAGGAGCAAAGATCCAGAAGTGCTACTCCGTCTTGAACAGCCTTCCCGGTTGTCTCTCCAAAGGAAAAAAGATGCAGAAGTTCTACCCAGCCAGGAACAGCCTTCCCAGTTGTCTCTCCAAAGGCACAAAGGTCCAGAGGTGCTACCCAGGAACTGCCTTCCCGGTTGTCTCTCCAAAGGCCTAAAGGTCCAGAAGTGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

274

Amino Acids

31.8

Weight (kDa)

9.57

Isoelectric Point (pI)

79.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 219
AccB1I GGYRCC 1 cut(s) 219
AccII CGCG 1 cut(s) 54
AciI CCGC 1 cut(s) 52
AclWI GGATC 9 cut(s) 43, 56, 415, 428, 443, 503, 563, 576, 623
AcuI CTGAAG 1 cut(s) 321
AfaI GTAC 4 cut(s) 20, 35, 221, 241
AfiI CCNNNNNNNGG 3 cut(s) 29, 58, 389
AgeI ACCGGT 1 cut(s) 603
AgsI TTSAA 3 cut(s) 437, 617, 653
AjnI CCWGG 3 cut(s) 28, 708, 764
AluBI AGCT 1 cut(s) 82
AluI AGCT 1 cut(s) 82
Alw26I GTCTC 4 cut(s) 9, 675, 735, 791
AlwI GGATC 9 cut(s) 43, 56, 415, 428, 443, 503, 563, 576, 623
AlwNI CAGNNNCTG 1 cut(s) 771
Ama87I CYCGRG 1 cut(s) 156
AoxI GGCC 4 cut(s) 60, 102, 292, 796
ApeKI GCWGC 1 cut(s) 475
AsiGI ACCGGT 1 cut(s) 603
Asp718I GGTACC 1 cut(s) 219
AspS9I GGNCC 3 cut(s) 61, 749, 805
AsuC2I CCSGG 2 cut(s) 664, 780
AvaI CYCGRG 1 cut(s) 156
AvaII GGWCC 2 cut(s) 749, 805
BamHI GGATCC 3 cut(s) 48, 420, 568
BanI GGYRCC 1 cut(s) 219
BauI CACGAG 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 487
BccI CCATC 1 cut(s) 383
BciT130I CCWGG 3 cut(s) 30, 710, 766
BcnI CCSGG 2 cut(s) 664, 780
BcoDI GTCTC 4 cut(s) 9, 675, 735, 791
BfaI CTAG 1 cut(s) 540
BisI GCNGC 1 cut(s) 476
BlsI GCNGC 1 cut(s) 477
Bme1390I CCNGG 5 cut(s) 30, 664, 710, 766, 780
Bme18I GGWCC 2 cut(s) 749, 805
BmeT110I CYCGRG 1 cut(s) 156
BmgT120I GGNCC 3 cut(s) 61, 749, 805
BmiI GGNNCC 6 cut(s) 50, 171, 221, 325, 422, 570
BmrFI CCNGG 5 cut(s) 30, 664, 710, 766, 780
BmrI ACTGGG 1 cut(s) 718
BmsI GCATC 2 cut(s) 507, 679
BmuI ACTGGG 1 cut(s) 718
BpiI GAAGAC 1 cut(s) 234
BpuMI CCSGG 2 cut(s) 664, 780
Bsa29I ATCGAT 1 cut(s) 69
BsaAI YACGTR 1 cut(s) 37
BsaJI CCNNGG 3 cut(s) 29, 247, 764
BsaWI WCCGGW 2 cut(s) 423, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 58, 389
Bse118I RCCGGY 1 cut(s) 603
Bse1I ACTGG 6 cut(s) 317, 484, 512, 544, 572, 724
BseBI CCWGG 3 cut(s) 30, 710, 766
BseCI ATCGAT 1 cut(s) 69
BseDI CCNNGG 3 cut(s) 29, 247, 764
BseLI CCNNNNNNNGG 3 cut(s) 29, 58, 389
BseNI ACTGG 6 cut(s) 317, 484, 512, 544, 572, 724
BseRI GAGGAG 2 cut(s) 15, 485
BseXI GCAGC 1 cut(s) 487
BseYI CCCAGC 1 cut(s) 704
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 4 cut(s) 62, 104, 294, 798
BshNI GGYRCC 1 cut(s) 219
BshTI ACCGGT 1 cut(s) 603
BshVI ATCGAT 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 4 cut(s) 424, 604, 664, 780
BslI CCNNNNNNNGG 3 cut(s) 29, 58, 389
BsmAI GTCTC 4 cut(s) 9, 675, 735, 791
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 4 cut(s) 62, 104, 294, 798
BsoBI CYCGRG 1 cut(s) 156
Bsp143I GATC 7 cut(s) 48, 66, 420, 448, 508, 568, 628
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 1 cut(s) 52
BspANI GGCC 4 cut(s) 62, 104, 294, 798
BspDI ATCGAT 1 cut(s) 69
BspFNI CGCG 1 cut(s) 54
BspLI GGNNCC 6 cut(s) 50, 171, 221, 325, 422, 570
BspPI GGATC 9 cut(s) 43, 56, 415, 428, 443, 503, 563, 576, 623
BspT107I GGYRCC 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 603
BsrI ACTGG 6 cut(s) 317, 484, 512, 544, 572, 724
BssAI RCCGGY 1 cut(s) 603
BssECI CCNNGG 3 cut(s) 29, 247, 764
BssMI GATC 7 cut(s) 48, 66, 420, 448, 508, 568, 628
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 247
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 3 cut(s) 30, 710, 766
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 4 cut(s) 113, 251, 340, 438
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 395, 409
BstDSI CCRYGG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 7 cut(s) 51, 69, 423, 451, 511, 571, 631
BstMAI GTCTC 4 cut(s) 9, 675, 735, 791
BstMBI GATC 7 cut(s) 48, 66, 420, 448, 508, 568, 628
BstNI CCWGG 3 cut(s) 30, 710, 766
BstSCI CCNGG 5 cut(s) 28, 662, 708, 764, 778
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 1 cut(s) 487
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 6 cut(s) 48, 420, 448, 508, 568, 628
BstYI RGATCY 6 cut(s) 48, 420, 448, 508, 568, 628
Bsu15I ATCGAT 1 cut(s) 69
BsuRI GGCC 4 cut(s) 62, 104, 294, 798
BsuTUI ATCGAT 1 cut(s) 69
BtgI CCRYGG 1 cut(s) 247
BtgZI GCGATG 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 2 cut(s) 395, 409
CaiI CAGNNNCTG 1 cut(s) 771
Cfr10I RCCGGY 1 cut(s) 603
Cfr13I GGNCC 3 cut(s) 61, 749, 805
ClaI ATCGAT 1 cut(s) 69
Csp6I GTAC 4 cut(s) 19, 34, 220, 240
CspAI ACCGGT 1 cut(s) 603
CviAII CATG 2 cut(s) 248, 266
CviQI GTAC 4 cut(s) 19, 34, 220, 240
DpnI GATC 7 cut(s) 50, 68, 422, 450, 510, 570, 630
DpnII GATC 7 cut(s) 48, 66, 420, 448, 508, 568, 628
Eam1104I CTCTTC 4 cut(s) 113, 251, 340, 438
EarI CTCTTC 4 cut(s) 113, 251, 340, 438
Eco130I CCWWGG 1 cut(s) 247
Eco147I AGGCCT 1 cut(s) 798
Eco47I GGWCC 2 cut(s) 749, 805
Eco57I CTGAAG 1 cut(s) 321
Eco88I CYCGRG 1 cut(s) 156
EcoO109I RGGNCCY 1 cut(s) 61
EcoRII CCWGG 3 cut(s) 28, 708, 764
EcoT14I CCWWGG 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 247
FaeI CATG 2 cut(s) 251, 269
FaiI YATR 4 cut(s) 195, 249, 267, 399
FatI CATG 2 cut(s) 247, 265
Fnu4HI GCNGC 1 cut(s) 476
Fsp4HI GCNGC 1 cut(s) 476
FspBI CTAG 1 cut(s) 540
GluI GCNGC 1 cut(s) 476
GsaI CCCAGC 1 cut(s) 708
HaeIII GGCC 4 cut(s) 62, 104, 294, 798
HapII CCGG 4 cut(s) 424, 604, 664, 780
Hin1II CATG 2 cut(s) 251, 269
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 3 cut(s) 71, 190, 377
HpaI GTTAAC 1 cut(s) 352
HpaII CCGG 4 cut(s) 424, 604, 664, 780
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 2 cut(s) 340, 420
Hpy188III TCNNGA 7 cut(s) 158, 452, 525, 632, 650, 752, 808
Hpy8I GTNNAC 1 cut(s) 352
HpyAV CCTTC 6 cut(s) 37, 94, 145, 669, 729, 785
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 2 cut(s) 407, 692
HpySE526I ACGT 1 cut(s) 36
Hsp92II CATG 2 cut(s) 251, 269
KpnI GGTACC 1 cut(s) 223
KspAI GTTAAC 1 cut(s) 352
Kzo9I GATC 7 cut(s) 48, 66, 420, 448, 508, 568, 628
LmnI GCTCC 4 cut(s) 87, 169, 472, 621
Lsp1109I GCAGC 1 cut(s) 487
LweI GCATC 2 cut(s) 507, 679
MaeI CTAG 1 cut(s) 540
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 1 cut(s) 609
MalI GATC 7 cut(s) 50, 68, 422, 450, 510, 570, 630
MboI GATC 7 cut(s) 48, 66, 420, 448, 508, 568, 628
MflI RGATCY 6 cut(s) 48, 420, 448, 508, 568, 628
MluCI AATT 1 cut(s) 400
MlyI GAGTC 2 cut(s) 199, 371
MnlI CCTC 9 cut(s) 33, 36, 52, 114, 441, 463, 561, 583, 748
MseI TTAA 2 cut(s) 78, 351
MslI CAYNNNNRTG 1 cut(s) 210
MspI CCGG 4 cut(s) 424, 604, 664, 780
MspR9I CCNGG 5 cut(s) 30, 664, 710, 766, 780
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 3 cut(s) 30, 710, 766
MvnI CGCG 1 cut(s) 54
NciI CCSGG 2 cut(s) 664, 780
NcoI CCATGG 1 cut(s) 247
NdeII GATC 7 cut(s) 48, 66, 420, 448, 508, 568, 628
NlaIII CATG 2 cut(s) 251, 269
NlaIV GGNNCC 6 cut(s) 50, 171, 221, 325, 422, 570
NmuCI GTSAC 1 cut(s) 609
PaeR7I CTCGAG 1 cut(s) 156
PceI AGGCCT 1 cut(s) 798
PctI GAATGC 1 cut(s) 411
PfeI GAWTC 1 cut(s) 71
PinAI ACCGGT 1 cut(s) 603
PkrI GCNGC 1 cut(s) 477
PleI GAGTC 2 cut(s) 198, 371
PpsI GAGTC 2 cut(s) 198, 371
Ppu21I YACGTR 1 cut(s) 37
Psp6I CCWGG 3 cut(s) 28, 708, 764
PspFI CCCAGC 1 cut(s) 704
PspGI CCWGG 3 cut(s) 28, 708, 764
PspN4I GGNNCC 6 cut(s) 50, 171, 221, 325, 422, 570
PspPI GGNCC 3 cut(s) 61, 749, 805
PstNI CAGNNNCTG 1 cut(s) 771
PsuI RGATCY 6 cut(s) 48, 420, 448, 508, 568, 628
RsaI GTAC 4 cut(s) 20, 35, 221, 241
RsaNI GTAC 4 cut(s) 19, 34, 220, 240
RseI CAYNNNNRTG 1 cut(s) 210
SaqAI TTAA 2 cut(s) 78, 351
SatI GCNGC 1 cut(s) 476
Sau3AI GATC 7 cut(s) 48, 66, 420, 448, 508, 568, 628
Sau96I GGNCC 3 cut(s) 61, 749, 805
SchI GAGTC 2 cut(s) 199, 371
ScrFI CCNGG 5 cut(s) 30, 664, 710, 766, 780
SetI ASST 9 cut(s) 39, 84, 221, 241, 282, 541, 751, 759, 807
SfaNI GCATC 2 cut(s) 507, 679
Sfr274I CTCGAG 1 cut(s) 156
SinI GGWCC 2 cut(s) 749, 805
SlaI CTCGAG 1 cut(s) 156
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 156
SmoI CTYRAG 1 cut(s) 156
Sse9I AATT 1 cut(s) 400
SseBI AGGCCT 1 cut(s) 798
SsiI CCGC 1 cut(s) 52
SspMI CTAG 1 cut(s) 540
StuI AGGCCT 1 cut(s) 798
StyD4I CCNGG 5 cut(s) 28, 662, 708, 764, 778
StyI CCWWGG 1 cut(s) 247
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 1 cut(s) 39
TaqI TCGA 3 cut(s) 69, 74, 157
TasI AATT 1 cut(s) 400
TfiI GAWTC 1 cut(s) 71
Tru1I TTAA 2 cut(s) 78, 351
Tru9I TTAA 2 cut(s) 78, 351
TscAI CASTG 1 cut(s) 519
TseFI GTSAC 1 cut(s) 609
TseI GCWGC 1 cut(s) 475
Tsp45I GTSAC 1 cut(s) 609
TspDTI ATGAA 2 cut(s) 228, 282
TspGWI ACGGA 3 cut(s) 6, 287, 635
TspRI CASTG 1 cut(s) 519
VpaK11BI GGWCC 2 cut(s) 749, 805
XhoI CTCGAG 1 cut(s) 156
XspI CTAG 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.