pycom15g24240

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008830
Physical Location & Seq
Reverse (-)
18441068 .. 18441949
882 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 882 bp
ATGTCGTCGTCGTCAAACTCTCCTTGCGCAGCGTGCAAATTTCTACGGCGGAAGTGCACGCAGGAGTGCGTCTTCGCGCCCTACTTCCCACCTGACAACCCCCATAAGTTTTCCAAAGTCCACAAGGTCTTCGGTGCCAGCAATGTCGCCAAGATCCTCAACGAACTCAGCGCTTCTCAGCGTGATGACGCCGTCAACTCTCTGGCCTATGAGGCGGAGGCCCGCCTTCGCGACCCCGTCTACGGCTGCGTTGGACTCATTTCCATCCTTCAGCAGCGGCTCAAGGAAGTCCAGGCCGACCTTTTCAATGCCAAGAAGGAGCTCGCCACTTACATGGGCCCACAAGCTATGCTCTCCATCCTCCAGCCGCCAACTTACATGGCTCAGCAGCTCCAAGGAAACGCTTTTTCCGCCGTTTCGCCGTACATGTCTCCGATGATTGGCATTCCAACCGGGGCTGCTTCCGGTCAGTTGATGATTCGAGAGCCACAGCAGCAGCAGATTTTTGAGGCGCAGCAGTTGGCGGTGGCCGTGGCAGCGAGGGAGCAGCAGGCGCAGGACCATGTGAGATTCAACAGTGGGTTTGACTCGTCCCCTGGCTCGGTCACTGTGGGCGGCTTCACTCAGATGGCCGGAGCGTTGGGCAACGCCGTTTCGCCGTCTTTGGCTCTGGGGTTTGATAGCGGTCCGTACCACCAGATTCAGCAGCAGCAGCCTCACCATAATCCCCACCTCCACCTGCAGCTTCAGTCTCAGCTGTTGCTCCAAACCCAACAGCCGCAAGGGCAGCATCAGGCTCAGCAGTCCCAGCAACCCCAGCTGCAGGCAAAATCCGATCCGGGAGAGGAAGGCGGTGGTAGGAGTGTGGGTCCCTCTTGCTGA

Protein Analysis

294

Amino Acids

31.7

Weight (kDa)

7.11

Isoelectric Point (pI)

73.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 8 - 105 5.4e-41 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 747
Acc16I TGCGCA 1 cut(s) 28
Acc36I ACCTGC 1 cut(s) 747
AccB1I GGYRCC 1 cut(s) 134
AccI GTMKAC 1 cut(s) 240
AccII CGCG 2 cut(s) 77, 231
AclWI GGATC 2 cut(s) 148, 830
AcoI YGGCCR 2 cut(s) 528, 630
AcsI RAATTY 1 cut(s) 38
AcuI CTGAAG 2 cut(s) 254, 731
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 2 cut(s) 425, 692
AfeI AGCGCT 1 cut(s) 172
AfiI CCNNNNNNNGG 4 cut(s) 242, 440, 601, 823
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 2 cut(s) 307, 574
AjnI CCWGG 2 cut(s) 291, 595
AluBI AGCT 6 cut(s) 322, 347, 391, 745, 757, 820
AluI AGCT 6 cut(s) 322, 347, 391, 745, 757, 820
Alw21I GWGCWC 2 cut(s) 59, 324
Alw26I GTCTC 2 cut(s) 435, 756
Alw44I GTGCAC 1 cut(s) 55
AlwI GGATC 2 cut(s) 148, 830
Aor51HI AGCGCT 1 cut(s) 172
AoxI GGCC 6 cut(s) 204, 219, 294, 337, 528, 630
ApaI GGGCCC 1 cut(s) 341
ApaLI GTGCAC 1 cut(s) 55
ApoI RAATTY 1 cut(s) 38
AspLEI GCGC 5 cut(s) 29, 79, 173, 514, 556
AspS9I GGNCC 6 cut(s) 220, 337, 338, 559, 686, 869
AsuC2I CCSGG 2 cut(s) 454, 840
AsuHPI GGTGA 1 cut(s) 710
AvaII GGWCC 3 cut(s) 559, 686, 869
BaeGI GKGCMC 2 cut(s) 59, 341
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 2 cut(s) 324, 341
BbsI GAAGAC 2 cut(s) 64, 121
Bbv12I GWGCWC 2 cut(s) 59, 324
BccI CCATC 3 cut(s) 272, 365, 622
BceAI ACGGC 8 cut(s) 62, 176, 259, 398, 406, 515, 635, 643
BciT130I CCWGG 2 cut(s) 293, 597
BcnI CCSGG 2 cut(s) 454, 840
BcoDI GTCTC 2 cut(s) 435, 756
BfmI CTRYAG 2 cut(s) 740, 821
BfoI RGCGCY 1 cut(s) 174
BfuAI ACCTGC 1 cut(s) 747
BglI GCCNNNNNGGC 2 cut(s) 212, 784
BlpI GCTNAGC 2 cut(s) 384, 798
Bme1390I CCNGG 4 cut(s) 293, 454, 597, 840
Bme18I GGWCC 3 cut(s) 559, 686, 869
BmgT120I GGNCC 6 cut(s) 220, 337, 338, 559, 686, 869
BmiI GGNNCC 4 cut(s) 136, 339, 870, 871
BmrFI CCNGG 4 cut(s) 293, 454, 597, 840
BmsI GCATC 1 cut(s) 799
BpiI GAAGAC 2 cut(s) 64, 121
BpmI CTGGAG 1 cut(s) 347
Bpu1102I GCTNAGC 2 cut(s) 384, 798
BpuEI CTTGAG 1 cut(s) 266
BpuMI CCSGG 2 cut(s) 454, 840
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 4 cut(s) 394, 453, 531, 595
BsaWI WCCGGW 1 cut(s) 464
BsaXI ACNNNNNCTCC 1 cut(s) 853
Bsc4I CCNNNNNNNGG 4 cut(s) 242, 440, 601, 823
Bse3DI GCAATG 1 cut(s) 148
BseBI CCWGG 2 cut(s) 293, 597
BseDI CCNNGG 4 cut(s) 394, 453, 531, 595
BseGI GGATG 2 cut(s) 264, 357
BseLI CCNNNNNNNGG 4 cut(s) 242, 440, 601, 823
BseMI GCAATG 1 cut(s) 148
BseMII CTCAG 6 cut(s) 181, 191, 398, 638, 767, 812
BseSI GKGCMC 2 cut(s) 59, 341
BseYI CCCAGC 2 cut(s) 807, 816
Bsh1236I CGCG 2 cut(s) 77, 231
BshFI GGCC 6 cut(s) 206, 221, 296, 339, 530, 632
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 59, 324
BsiSI CCGG 4 cut(s) 453, 465, 633, 839
BslFI GGGAC 3 cut(s) 577, 790, 855
BslI CCNNNNNNNGG 4 cut(s) 242, 440, 601, 823
BsmAI GTCTC 2 cut(s) 435, 756
BsmFI GGGAC 3 cut(s) 577, 790, 855
BsmI GAATGC 1 cut(s) 444
BsnI GGCC 6 cut(s) 206, 221, 296, 339, 530, 632
Bsp120I GGGCCC 1 cut(s) 337
Bsp1286I GDGCHC 3 cut(s) 59, 324, 341
Bsp143I GATC 2 cut(s) 153, 835
Bsp1720I GCTNAGC 2 cut(s) 384, 798
Bsp68I TCGCGA 1 cut(s) 231
BspANI GGCC 6 cut(s) 206, 221, 296, 339, 530, 632
BspCNI CTCAG 6 cut(s) 180, 190, 397, 637, 766, 811
BspFNI CGCG 2 cut(s) 77, 231
BspLI GGNNCC 4 cut(s) 136, 339, 870, 871
BspMAI CTGCAG 2 cut(s) 744, 825
BspMI ACCTGC 1 cut(s) 747
BspPI GGATC 2 cut(s) 148, 830
BspT107I GGYRCC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 148
BssECI CCNNGG 4 cut(s) 394, 453, 531, 595
BssMI GATC 2 cut(s) 153, 835
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 2 cut(s) 293, 597
Bst4CI ACNGT 2 cut(s) 578, 610
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 7 cut(s) 34, 59, 139, 223, 324, 552, 825
BstDEI CTNAG 6 cut(s) 167, 177, 384, 624, 753, 798
BstDSI CCRYGG 1 cut(s) 531
BstF5I GGATG 2 cut(s) 264, 357
BstFNI CGCG 2 cut(s) 77, 231
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 5 cut(s) 29, 79, 173, 514, 556
BstKTI GATC 2 cut(s) 156, 838
BstMAI GTCTC 2 cut(s) 435, 756
BstMBI GATC 2 cut(s) 153, 835
BstNI CCWGG 2 cut(s) 293, 597
BstNSI RCATGY 1 cut(s) 430
BstSCI CCNGG 4 cut(s) 291, 452, 595, 838
BstSFI CTRYAG 2 cut(s) 740, 821
BstSLI GKGCMC 2 cut(s) 59, 341
BstUI CGCG 2 cut(s) 77, 231
BstV2I GAAGAC 2 cut(s) 64, 121
BstX2I RGATCY 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 334
BstYI RGATCY 1 cut(s) 153
BsuRI GGCC 6 cut(s) 206, 221, 296, 339, 530, 632
BtgI CCRYGG 1 cut(s) 531
BtsCI GGATG 2 cut(s) 264, 357
BtsIMutI CAGTG 2 cut(s) 583, 606
BtuMI TCGCGA 1 cut(s) 231
BveI ACCTGC 1 cut(s) 747
Cac8I GCNNGC 7 cut(s) 34, 59, 139, 223, 324, 552, 825
CfoI GCGC 5 cut(s) 29, 79, 173, 514, 556
Cfr13I GGNCC 6 cut(s) 220, 337, 338, 559, 686, 869
CpoI CGGWCCG 1 cut(s) 686
CseI GACGC 2 cut(s) 58, 197
Csp6I GTAC 2 cut(s) 424, 691
CspI CGGWCCG 1 cut(s) 686
CviAII CATG 4 cut(s) 334, 379, 427, 563
CviQI GTAC 2 cut(s) 424, 691
DdeI CTNAG 6 cut(s) 167, 177, 384, 624, 753, 798
DpnI GATC 2 cut(s) 155, 837
DpnII GATC 2 cut(s) 153, 835
EaeI YGGCCR 2 cut(s) 528, 630
EciI GGCGGA 3 cut(s) 64, 230, 400
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 394
Eco24I GRGCYC 2 cut(s) 324, 341
Eco47I GGWCC 3 cut(s) 559, 686, 869
Eco47III AGCGCT 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 2 cut(s) 254, 731
EcoICRI GAGCTC 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 869
EcoRII CCWGG 2 cut(s) 291, 595
EcoT14I CCWWGG 1 cut(s) 394
EcoT38I GRGCYC 2 cut(s) 324, 341
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 4 cut(s) 337, 382, 430, 566
FaiI YATR 8 cut(s) 105, 210, 335, 350, 380, 428, 564, 723
FaqI GGGAC 3 cut(s) 577, 790, 855
FatI CATG 4 cut(s) 333, 378, 426, 562
FauI CCCGC 1 cut(s) 230
FblI GTMKAC 1 cut(s) 240
FokI GGATG 2 cut(s) 251, 344
FriOI GRGCYC 2 cut(s) 324, 341
FspI TGCGCA 1 cut(s) 28
GlaI GCGC 5 cut(s) 28, 78, 172, 513, 555
GsaI CCCAGC 2 cut(s) 811, 820
GsuI CTGGAG 1 cut(s) 347
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 6 cut(s) 206, 221, 296, 339, 530, 632
HapII CCGG 4 cut(s) 453, 465, 633, 839
HgaI GACGC 2 cut(s) 58, 197
HhaI GCGC 5 cut(s) 29, 79, 173, 514, 556
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 4 cut(s) 337, 382, 430, 566
Hin6I GCGC 5 cut(s) 27, 77, 171, 512, 554
HinP1I GCGC 5 cut(s) 27, 77, 171, 512, 554
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 5 cut(s) 255, 478, 570, 587, 700
HpaII CCGG 4 cut(s) 453, 465, 633, 839
HphI GGTGA 1 cut(s) 710
Hpy166II GTNNAC 4 cut(s) 57, 121, 196, 241
Hpy188I TCNGA 3 cut(s) 435, 627, 835
Hpy188III TCNNGA 2 cut(s) 230, 482
Hpy8I GTNNAC 4 cut(s) 57, 121, 196, 241
Hpy99I CGWCG 2 cut(s) 10, 13
HpyAV CCTTC 4 cut(s) 236, 278, 310, 842
HpyCH4III ACNGT 2 cut(s) 578, 610
HpyCH4V TGCA 4 cut(s) 36, 57, 742, 823
HpyF3I CTNAG 6 cut(s) 167, 177, 384, 624, 753, 798
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 4 cut(s) 337, 382, 430, 566
HspAI GCGC 5 cut(s) 27, 77, 171, 512, 554
KflI GGGWCCC 1 cut(s) 869
Kzo9I GATC 2 cut(s) 153, 835
LmnI GCTCC 5 cut(s) 319, 396, 544, 635, 768
LweI GCATC 1 cut(s) 799
MaeIII GTNAC 1 cut(s) 604
MalI GATC 2 cut(s) 155, 837
MboI GATC 2 cut(s) 153, 835
MboII GAAGA 2 cut(s) 64, 121
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 3 cut(s) 59, 324, 341
MluCI AATT 1 cut(s) 38
MlyI GAGTC 2 cut(s) 249, 581
MmeI TCCRAC 2 cut(s) 232, 473
MnlI CCTC 9 cut(s) 167, 205, 211, 371, 502, 534, 726, 743, 838
MslI CAYNNNNRTG 2 cut(s) 332, 626
MspA1I CMGCKG 3 cut(s) 277, 757, 820
MspI CCGG 4 cut(s) 453, 465, 633, 839
MspR9I CCNGG 4 cut(s) 293, 454, 597, 840
Mva1269I GAATGC 1 cut(s) 444
MvaI CCWGG 2 cut(s) 293, 597
MvnI CGCG 2 cut(s) 77, 231
NciI CCSGG 2 cut(s) 454, 840
NdeII GATC 2 cut(s) 153, 835
NlaIII CATG 4 cut(s) 337, 382, 430, 566
NlaIV GGNNCC 4 cut(s) 136, 339, 870, 871
NmuCI GTSAC 1 cut(s) 604
NruI TCGCGA 1 cut(s) 231
NsbI TGCGCA 1 cut(s) 28
NspI RCATGY 1 cut(s) 430
PaqCI CACCTGC 1 cut(s) 747
PciI ACATGT 1 cut(s) 426
PctI GAATGC 1 cut(s) 444
PfeI GAWTC 3 cut(s) 478, 570, 700
PflFI GACNNNGTC 2 cut(s) 191, 236
PfoI TCCNGGA 1 cut(s) 838
PleI GAGTC 2 cut(s) 249, 581
PpsI GAGTC 2 cut(s) 249, 581
PpuMI RGGWCCY 1 cut(s) 869
PscI ACATGT 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 2 cut(s) 291, 595
PspFI CCCAGC 2 cut(s) 807, 816
PspGI CCWGG 2 cut(s) 291, 595
PspN4I GGNNCC 4 cut(s) 136, 339, 870, 871
PspOMI GGGCCC 1 cut(s) 337
PspPI GGNCC 6 cut(s) 220, 337, 338, 559, 686, 869
PspPPI RGGWCCY 1 cut(s) 869
PstI CTGCAG 2 cut(s) 744, 825
PsuI RGATCY 1 cut(s) 153
PsyI GACNNNGTC 2 cut(s) 191, 236
PvuII CAGCTG 2 cut(s) 757, 820
RruI TCGCGA 1 cut(s) 231
RsaI GTAC 2 cut(s) 425, 692
RsaNI GTAC 2 cut(s) 424, 691
RseI CAYNNNNRTG 2 cut(s) 332, 626
Rsr2I CGGWCCG 1 cut(s) 686
RsrII CGGWCCG 1 cut(s) 686
SacI GAGCTC 1 cut(s) 324
Sau3AI GATC 2 cut(s) 153, 835
Sau96I GGNCC 6 cut(s) 220, 337, 338, 559, 686, 869
SchI GAGTC 2 cut(s) 249, 581
ScrFI CCNGG 4 cut(s) 293, 454, 597, 840
SduI GDGCHC 3 cut(s) 59, 324, 341
SfaNI GCATC 1 cut(s) 799
SfcI CTRYAG 2 cut(s) 740, 821
SinI GGWCC 3 cut(s) 559, 686, 869
SmiMI CAYNNNNRTG 2 cut(s) 332, 626
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 1 cut(s) 38
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 4 cut(s) 291, 452, 595, 838
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 2 cut(s) 578, 610
TaqI TCGA 1 cut(s) 481
TaqII GACCGA 1 cut(s) 592
TasI AATT 1 cut(s) 38
TauI GCSGC 4 cut(s) 280, 370, 618, 781
TfiI GAWTC 3 cut(s) 478, 570, 700
TscAI CASTG 2 cut(s) 583, 613
TseFI GTSAC 1 cut(s) 604
Tsp45I GTSAC 1 cut(s) 604
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 2 cut(s) 583, 613
Tth111I GACNNNGTC 2 cut(s) 191, 236
VneI GTGCAC 1 cut(s) 55
VpaK11BI GGWCC 3 cut(s) 559, 686, 869
XapI RAATTY 1 cut(s) 38
XceI RCATGY 1 cut(s) 430
XmiI GTMKAC 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.